CN106687585B - 用于修饰基因组dna的方法和组合物 - Google Patents

用于修饰基因组dna的方法和组合物 Download PDF

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CN106687585B
CN106687585B CN201580025136.0A CN201580025136A CN106687585B CN 106687585 B CN106687585 B CN 106687585B CN 201580025136 A CN201580025136 A CN 201580025136A CN 106687585 B CN106687585 B CN 106687585B
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electroporation
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CN106687585A (zh
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李林鸿
马杜苏登·佩什瓦
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Maxcyte Inc
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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CN201580025136.0A 2014-04-14 2015-04-13 用于修饰基因组dna的方法和组合物 Active CN106687585B (zh)

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US201461979178P 2014-04-14 2014-04-14
US61/979,178 2014-04-14
US201462078706P 2014-11-12 2014-11-12
US62/078,706 2014-11-12
PCT/US2015/025523 WO2015160683A1 (en) 2014-04-14 2015-04-13 Methods and compositions for modifying genomic dna

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US20170029805A1 (en) 2017-02-02
EP3132025B1 (en) 2023-08-30
EP3132025A1 (en) 2017-02-22
CN106687585A (zh) 2017-05-17
SG11201608503UA (en) 2016-11-29
KR20170026332A (ko) 2017-03-08
ES2962509T3 (es) 2024-03-19
WO2015160683A1 (en) 2015-10-22
JP7209671B2 (ja) 2023-01-20
KR102406585B1 (ko) 2022-06-07
JP2021010366A (ja) 2021-02-04
EP3132025A4 (en) 2017-10-18

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