CN106673851A - Radix astragali seu hedysari edible mushroom culture medium and method for cultivating radix astragali seu hedysari edible mushrooms by utilizing culture medium - Google Patents
Radix astragali seu hedysari edible mushroom culture medium and method for cultivating radix astragali seu hedysari edible mushrooms by utilizing culture medium Download PDFInfo
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- CN106673851A CN106673851A CN201710004724.7A CN201710004724A CN106673851A CN 106673851 A CN106673851 A CN 106673851A CN 201710004724 A CN201710004724 A CN 201710004724A CN 106673851 A CN106673851 A CN 106673851A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a radix astragali seu hedysari edible mushroom culture medium and a method for cultivating the radix astragali seu hedysari edible mushrooms by utilizing the culture medium. The radix astragali seu hedysari edible mushroom culture medium is prepared from the following raw materials in parts by weight: 30 to 70 parts of radix astragali seu hedysari leftover, 280 to 350 parts of sawdust and/or radix astragali seu hedysari stem, 50 to 100 parts of wheat bran, 5 to 20 parts of soybean flour and 2 to 8 parts of lime. The fresh mushroom yield of the radix astragali seu hedysari edible mushrooms cultivated by the radix astragali seu hedysari edible mushroom culture medium is 1.60kg/kg to 2.04kg/kg of dry materials and is increased by 6.7 percent to 32.1 percent when being compared with that of a culture medium without radix astragali seu hedysari; the content of effective nutrient components is also obviously improved when being compared with that of common edible mushrooms.
Description
Technical field
The present invention relates to a kind of Radix Astragali culture medium of edible fungus with medical value and using culture medium cultivation Radix Astragali food
With the method for bacterium, belong to mushroom cultivation technical field.
Background technology
Mushroom is a kind of high protein, low fat and the nutraceutical containing several mineral materials, and in today's society health is focused on
On the premise of quality of the life, high-quality, the edible fungi of high nutrition are also more favored, and in mushroom culture medium Chinese herbal medicine is added, and make
The edible fungi that culture is obtained has higher nutritive value and medical value, reaches the effect of " integration of edible and medicinal herbs ".Accordingly, in mushroom
Add the Radix Astragali in culture medium, the Radix Astragali edible fungi of the rich in nutrition content such as the aminoacid of acquisition, protein and polysaccharide has very
Good medicinal curative effect.But, the membership that adds of the Radix Astragali suppresses mycelial growth, the corresponding growth rate for reducing edible fungi, in actual cultivation
During training, often due to the compositional selecting and each component burden control to culture medium is improper, the mushroom production cycle can be caused
The long, underproduction and the result with abundant medical value Radix Astragali edible fungi can not be obtained.
The content of the invention
For problem above, the invention provides a kind of production efficiency is of a relatively high, yield is high, medicinal and be of high nutritive value
Radix Astragali culture medium of edible fungus and and the method for cultivating Radix Astragali edible fungi using the culture medium.
For achieving the above object, the present invention is employed the following technical solutions：
Radix Astragali edible fungi plantation culture medium raw material includes that weight portion is 30-70 part Radix Astragali leftover bits and pieces, 280-350 part wood flours
And/or Radix Astragali stalk, 50-100 part Testa Tritici, 5-20 part Semen Glycines powderes, 2-8 part calcium lime powders.The Radix Astragali leftover bits and pieces are ground into 1-3mm's
Granule mixing addition.
Described wood flour is to squeeze one or more in wood flour, birch bits or basswood bits.It is all or part of using Radix Astragali stalk
Substitute wood flour to use, it is possible to reduce the usage amount of Radix Astragali leftover bits and pieces, reduce expenses.
Described Radix Astragali culture medium of edible fungus also includes 0.1-5 part Gypsum Fibrosum.
Described Radix Astragali culture medium of edible fungus also includes 80-180 part cotton seed hullss.
The method for carrying out Radix Astragali edible fungus culturing using described Radix Astragali culture medium of edible fungus, comprises the following steps：
(1) Radix Astragali edible fungi parent species are made：2-8 part Radix Astragali leftover bits and pieces, 20-30 part Semen Maydis powder, 2-20 part Fructus Vitis viniferaes are taken respectively
Sugar, 20-30 part agar, add 900-1000 part water, are sufficiently mixed uniform, load culture test tube, it is sterilized after, obtain the Radix Astragali female
Plant culture medium；
The edible fungi parent species of high-quality are seeded to into the Radix Astragali mother culture media in sterile purification room after sterilizing, humidity keeps
Piece of tissue surrounding starts to grow the new mycelia of radial white after 60-70%, 24-26 DEG C of culture 48-50 hour of constant temperature, afterwards
The Jing 10-15 days cultures at a temperature of 25 DEG C ± 1 DEG C obtain Radix Astragali edible fungi parent species.
(2) Radix Astragali edible fungi original seed is made：The Radix Astragali leftover bits and pieces, wood flour and/or Huang are taken respectively according to the weight portion
Stilbene stalk, Testa Tritici, Semen Glycines powder and calcium lime powder, are sufficiently mixed uniformly, obtain mixed dry material；Add water in the mixed dry material and mixed
Wet feed is closed, amount of water is the 55-75% of the mixing wet feed weight, and full and uniform mixing, regulation pH value is 7-8, will be described mixed
Wet feed sterilizing is closed, the Radix Astragali culture medium of edible fungus is obtained final product；
Step (1) Radix Astragali edible fungi parent species are inoculated in the Radix Astragali culture medium of edible fungus, are carried out afterwards aseptic
After culture 15-20 days, Radix Astragali edible fungi original seed is obtained；The humidity of the aseptic culture be 60-70%, the temperature of the aseptic culture
Degree control is as follows：At 25 DEG C, temperature is down to 23-24 DEG C to front 3 days temperature controls within 4-10 days, drops to 8-22 DEG C within 11-20 days；
(3) bacterium is gone out：Radix Astragali edible fungi described in Radix Astragali culture medium of edible fungus inoculation step (2) obtained in step (2) is taken again
Original seed, it is 25-32 DEG C to control to purify room temperature, carries out mycelia culture, and mycelia enters physiological maturity within 30-40 days, then moves into out
Bacterium room enters bacterium culture, and total can pluck 2-4 stubble Radix Astragali edible fungi.Calculate from long fruiting flower bud or Auricularia urge ear to complete, it is whole
The individual fruiting cycle is 30-80 days.
Step (1) the edible fungi parent species are the artificial culture starter kind not comprising Caulis Bambusae In Taeniam bacterium and Tricholoma matsutake (lto et lmai) Singer parent species.
The raw material components of step (1) Radix Astragali mother culture media also add 0.1-1 parts magnesium sulfate and 0.1-2 part di(2-ethylhexyl)phosphates
Hydrogen potassium.
In step (1), the pressure of the sterilizing is 0.15-0.18MPa, and the temperature of the sterilizing is 120-130 DEG C, described
The time of sterilizing is 30-60 minutes.
In step (2), the sterilizing adopts normal-pressure sterilization, and the temperature of the sterilizing is 100-107 DEG C, the sterilizing when
Between be 8-14 hours.
In step (3), it is described go out bacterium room temperature be 10-25 DEG C, air humidity is 85-95%, and intensity of illumination is 50-
3500Lx。
In step (3), carry out it is described go out bacterium training orientation light application time be controlled to 2-8 hours/day, control light intensity and light
According to the time using sunshade net or solar energy dark slide.
Beneficial effect acquired by the present invention is：
Radix Astragali culture medium of edible fungus of the present invention is by with Radix Astragali leftover bits and pieces, wood flour and/or Radix Astragali stalk, Testa Tritici, bean
Powder, calcium lime powder are raw material and carry out approrpiate wts proportioning, and the Radix Astragali culture medium of edible fungus for preparing is adapted for Radix Astragali food
With the cultivation of bacterium, the Radix Astragali edible fungi quality for carrying out Radix Astragali edible fungus culturing acquisition using the Radix Astragali culture medium of edible fungus is carried
Height, fresh bacterium yield is that 1.60~2.04kg/kg siccatives (siccative is water-free culture medium raw material mixture) are not relatively added
Yield increases 6.7-32.1% during the Radix Astragali, and the more general edible fungi of effective nutritional labeling is also obviously improved：By detecting aminoacid
Quantity, it was demonstrated that its total amino acid content and Available Amino Acids accounting are significantly increased, total amino acid content increases 9.2-31.3%, must
Aminoacid accounting is needed to increase 4.7-15.4%, the nutrition of substance Radix Astragali edible fungi and medical value are high, good in economic efficiency.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical scheme will be carried out below
Detailed description.Obviously, described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.Base
Embodiment in the present invention, those of ordinary skill in the art are resulting on the premise of creative work is not made to be owned
Other embodiment, belongs to the scope that the present invention is protected.
1g is represented with 1 weight portion in example below.
Embodiment 1
The present embodiment cultivates Radix Astragali Lentinus Edodess.
The present embodiment provides a kind of Radix Astragali culture medium of edible fungus, including the raw material of following weight portion：
30 parts of Radix Astragali leftover bits and pieces, 350 parts of squeezing wood flours, 50 parts of Testa Tritici, 20 parts of Semen Glycines powderes, 2 parts of calcium lime powders.
The method for carrying out Radix Astragali cultivating champignon using the Radix Astragali culture medium of edible fungus, comprises the following steps：
(1) Radix Astragali Lentinus Edodess parent species are made：2 parts of Radix Astragali leftover bits and pieces, 30 parts of Semen Maydis powder, 10 parts of glucoses, 20 parts of fine jades are taken respectively
Fat, adds 900 parts of water, is sufficiently mixed the uniform rear culture test tube that loads and enters pressure cooker sterilizing, and pressure 0.15MPa, temperature is 120 DEG C
Insulation 60 minutes, after the completion of sterilizing, obtains Radix Astragali mother culture media, treats that kettle temperature is down to 60 DEG C, by Radix Astragali parent species training
Foster base removes pressure cooker, and temperature is down to 28 DEG C, and the Radix Astragali mother culture media is moved into into sterile purification room；
Lentinus Edodess parent species are inoculated into into the Radix Astragali mother culture media in sterile purification room, are 60%, 24 DEG C of constant temperature in humidity
Piece of tissue surrounding starts to grow the new mycelia of radial white after cultivating 48 hours, continues culture at 25 DEG C afterwards and obtains for 10 days
Radix Astragali Lentinus Edodess parent species；
(2) Radix Astragali Lentinus Edodess original seed is made：The Radix Astragali is taken respectively according to the Radix Astragali mushroom culture medium raw material weight to get a foothold
Material, wood flour, Testa Tritici, Semen Glycines powder and calcium lime powder are dry-mixed 20 minutes, obtain mixed dry material；552 parts are added in the mixed dry material
Water (amount of water account for mixing wet feed gross weight 55%), wet mixing 30 minutes, adjust pH be 7.8 after, obtain mix wet feed, often cultivate
The packed mixing wet feed 2.2kg, then moves into autoclave by culture bag, and the insulation at 107 DEG C carries out normal-pressure sterilization in 8 hours,
The Radix Astragali culture medium of edible fungus is obtained, kettle temperature is down to 60 DEG C, Radix Astragali culture medium of edible fungus is removed into autoclave, temperature drop
To 20 DEG C, Radix Astragali culture medium of edible fungus is moved into into sterile purification room；
It is female per culture bag Radix Astragali culture medium of edible fungus inoculation 5mL steps (1) Radix Astragali Lentinus Edodess in sterile purification room
Kind, aseptic culture is carried out afterwards 15 days, obtain Radix Astragali Lentinus Edodess original seed；The humidity of the aseptic culture is 70%, the aseptic training
Foster temperature control is as follows：At 25 DEG C, temperature is down to 23 DEG C to front 3 days temperature controls within 4-10 days, the cooling with 2 DEG C/day in 11-13 days
Speed is cooled to 17 DEG C, and the rate of temperature fall with 3 DEG C/day is cooled to 11 DEG C within 14-15 days；
(3) fruiting：The obtained culture bag equipped with the Radix Astragali culture medium of edible fungus of step (2) is taken again, and is moved into aseptic
Clean room, the Radix Astragali Lentinus Edodess original seed described in inoculation 10mL steps (2) per culture bag is cultivated 20 hours strains at 28 DEG C and is sprouted,
Enter back into sterile purification and educate bacterium room and be maintained at 28 DEG C, aseptic culture after 30 days mycelia enter physiological maturity, subsequently into aseptic
Mushroom producing room, carries out mushroom producing culture management, and it is 25 DEG C to control cultivation temperature on daytime, and night temperatures are 12 DEG C, and humidity 85%, winter is used
Sunshade net controls intensity of illumination 50Lx, and daily light application time is 2 hours, and 75 days long fruiting flower buds continue to cultivate 5 days, you can pluck
First batch of Radix Astragali Lentinus Edodes, always collects four batches, and the whole mushroom producing culture cycle is 60 days.
After testing, the present embodiment cultivation obtains the mushroom lid diameter of Radix Astragali Lentinus Edodess：9-11cm, equal fresh mushroom production：1.63kg/kg
Siccative, increases by 8.7%, total amino acid content accounting compared with Radix Astragali yield is not added with culture medium：(detecting after drying) 20.58%, it is necessary to
Aminoacid accounts for total amino acidss amount：36.77%.
Embodiment 2
The present embodiment cultivates Radix Astragali Lentinus Edodess.
The present embodiment provides a kind of Radix Astragali culture medium of edible fungus, including the raw material of following weight portion：
70 parts of Radix Astragali leftover bits and pieces, 280 parts of basswood bits, 100 parts of Testa Tritici, 5 parts of Semen Glycines powderes, 8 parts of calcium lime powders.
The method for carrying out Radix Astragali cultivating champignon using the Radix Astragali culture medium of edible fungus, comprises the following steps：
(1) Radix Astragali Lentinus Edodess parent species are made：8 parts of Radix Astragali leftover bits and pieces, 20 parts of Semen Maydis powder, 20 parts of glucoses, 20 parts of fine jades are taken respectively
Fat, adds 1000 parts of water, is sufficiently mixed the uniform rear culture test tube that loads and enters pressure cooker sterilizing, and pressure 0.18MPa, temperature is 130
DEG C insulation 30 minutes, after the completion of sterilizing, obtain Radix Astragali mother culture media, treat that kettle temperature is down to 65 DEG C, by the Radix Astragali parent species
Culture medium removes pressure cooker, and temperature is down to 25 DEG C, and the mother culture media is moved into into sterile purification room；
Lentinus Edodess parent species are inoculated into into the Radix Astragali mother culture media in sterile purification room, are 70% in humidity, 25 DEG C of constant temperature
Piece of tissue surrounding starts to grow the new mycelia of radial white after cultivating 50 hours, continues to cultivate Jing cultures in 15 days afterwards at 25 DEG C
Obtain Radix Astragali Lentinus Edodess parent species；
(2) Radix Astragali Lentinus Edodess original seed is made：The Radix Astragali is taken respectively according to the Radix Astragali mushroom culture medium raw material weight to get a foothold
Material, wood flour, Testa Tritici, Semen Glycines powder and calcium lime powder are dry-mixed 30 minutes, obtain mixed dry material；1389 parts are added in the mixed dry material
Water (amount of water account for mixing wet feed gross weight 75%), wet mixing 20 minutes, adjust pH be 7.2 after, obtain mix wet feed, often cultivate
The packed mixing wet feed 2.2kg, then moves into autoclave by culture bag, and the insulation at 100 DEG C carries out normal-pressure sterilization in 14 hours,
The Radix Astragali culture medium of edible fungus is obtained, kettle temperature is down to 60 DEG C, Radix Astragali culture medium of edible fungus is removed into autoclave, temperature drop
To 24 DEG C, Radix Astragali culture medium of edible fungus is moved into into sterile purification room；
It is female per culture bag Radix Astragali culture medium of edible fungus inoculation 5mL steps (1) Radix Astragali Lentinus Edodess in sterile purification room
Kind, aseptic culture is carried out afterwards 20 days, obtain Radix Astragali Lentinus Edodess original seed；The humidity of the aseptic culture is 70%, the aseptic training
Foster temperature control is as follows：At 25 DEG C, temperature is down to 23 DEG C to front 3 days temperature controls within 4-10 days, and temperature is down to 22 DEG C within 11-14 days,
Rate of temperature fall with 2 DEG C/day is cooled to 10 DEG C within 15-20 days；
(3) fruiting：The obtained culture bag equipped with the Radix Astragali culture medium of edible fungus of step (2) is taken again, and is moved into aseptic
Clean room, the Radix Astragali Lentinus Edodess original seed described in inoculation 20mL steps (2) per culture bag is cultivated 24 hours strains at 28 DEG C and is sprouted,
Enter back into sterile purification and educate bacterium room and be maintained at 24 DEG C, aseptic culture after 40 days mycelia enter physiological maturity, into aseptic fruiting
Room, carries out mushroom producing culture management, and it is 24 DEG C to control cultivation temperature on daytime, and night temperatures are 14 DEG C, humidity 95%, intensity of illumination
3500Lx, summer controlled light application time per daily sunshade net for 4 hours, and 90 days long fruiting flower buds continue to cultivate 7 days, you can pluck
First batch of Radix Astragali Lentinus Edodes, always collects four batches, and the whole mushroom producing culture cycle is 80 days.
After testing, the present embodiment cultivation obtains the mushroom lid diameter of Radix Astragali Lentinus Edodess：9-10cm, equal fresh mushroom production：1.60kg/kg
Siccative, compared with Radix Astragali yield is not added with culture medium 6.7% is increased, and total amino acid content accounting (is detected) after drying：24.75%, it is necessary to
Aminoacid accounts for total amino acidss amount：40.52%.
Embodiment 3
The present embodiment cultivates Radix Astragali Lentinus Edodess.
The present embodiment provides a kind of Radix Astragali culture medium of edible fungus, including the raw material of following weight portion：
50 parts of Radix Astragali leftover bits and pieces, 300 parts of squeezing wood flours, 80 parts of Testa Tritici, 10 parts of Semen Glycines powderes, 2.5 parts of calcium lime powders, 2.5 parts of Gypsum Fibrosum.
The method for carrying out Radix Astragali cultivating champignon using the Radix Astragali culture medium of edible fungus, comprises the following steps：
(1) Radix Astragali Lentinus Edodess parent species are made：5 parts of Radix Astragali leftover bits and pieces, 20 parts of Semen Maydis powder, 20 parts of glucoses, 20 parts of fine jades are taken respectively
Fat, 0.1 part of magnesium sulfate, 2 parts of potassium dihydrogen phosphates, add 1000 parts of water, are sufficiently mixed the uniform rear culture test tube that loads and enter pressure cooker
Sterilizing, pressure 0.16MPa, temperature is incubated 30 minutes for 126 DEG C, after the completion of sterilizing, obtains Radix Astragali mother culture media, treats a pot interior temperature
Degree is down to 65 DEG C, and the Radix Astragali mother culture media is removed into pressure cooker, and temperature is down to 25 DEG C, and the Radix Astragali mother culture media is moved
Enter sterile purification room；
Lentinus Edodess parent species are inoculated into into the Radix Astragali mother culture media in sterile purification room, are 70% in humidity, 26 DEG C of constant temperature
Piece of tissue surrounding starts to grow the new mycelia of radial white after cultivating 48 hours, continues to cultivate at 25 DEG C afterwards and cultivates for 12 days
To Radix Astragali Lentinus Edodess parent species；
(2) Radix Astragali Lentinus Edodess original seed is made：The Radix Astragali is taken respectively according to the Radix Astragali culture medium of edible fungus parts by weight of raw materials
Leftover bits and pieces, wood flour, Testa Tritici, Semen Glycines powder and calcium lime powder are dry-mixed 30 minutes, obtain mixed dry material；Add in the mixed dry material
668 parts of water (amount of water accounts for the 60% of mixing wet feed gross weight), wet mixing 20 minutes, it is after 7.8, to obtain mixing wet feed to adjust pH,
The packed mixing wet feed 2.2kg is often cultivated, then culture bag autoclave is moved into into, the insulation at 102 DEG C is carried out often for 12 hours
Pressure sterilizing, obtains the Radix Astragali culture medium of edible fungus, and kettle temperature is down to 70 DEG C and Radix Astragali culture medium of edible fungus is removed into autoclave,
Temperature is down to 25 DEG C, and Radix Astragali culture medium of edible fungus is moved into into sterile purification room；
It is female per culture bag Radix Astragali culture medium of edible fungus inoculation 5mL steps (1) Radix Astragali Lentinus Edodess in sterile purification room
Kind, aseptic culture is carried out afterwards 18 days, obtain Radix Astragali Lentinus Edodess original seed；The humidity of the aseptic culture is 70%, the aseptic training
Foster temperature control is as follows：At 25 DEG C, temperature is down to 23 DEG C to front 3 days temperature controls within 4-10 days, and temperature is down to 20 DEG C within 11-14 days,
Rate of temperature fall with 3 DEG C/day is cooled to 8 DEG C within 15-18 days；
(3) fruiting：The obtained culture bag equipped with the Radix Astragali culture medium of edible fungus of step (2) is taken again, and is moved into aseptic
Clean room, the Radix Astragali Lentinus Edodess original seed described in inoculation 15mL steps (2) per culture bag is cultivated 23 hours strains at 28 DEG C and is sprouted,
Enter back into sterile purification and educate bacterium room and be maintained at 25 DEG C, aseptic culture after 33 days mycelia enter physiological maturity, subsequently into aseptic
Mushroom producing room, carries out mushroom producing culture management, and it is 25 DEG C to control cultivation temperature on daytime, and night temperatures are 12 DEG C, and humidity 90%, summer is used
Solar energy sunshade plate controls intensity of illumination 700Lx, and daily light application time is 3 hours, and 82 days long fruiting flower buds continue culture 5 days, i.e.,
First batch of Radix Astragali Lentinus Edodes can be plucked, four batches are always collected, the whole mushroom producing culture cycle is 68 days.
After testing, the present embodiment cultivation obtains the mushroom lid diameter of Radix Astragali Lentinus Edodess：11-12cm, equal fresh mushroom production：1.82kg/
Kg siccatives, compared with Radix Astragali yield is not added with culture medium 21.3% is increased, and total amino acid content accounting (is detected) after drying：23.46%, must
Aminoacid is needed to account for total amino acidss amount：39.84%.
Embodiment 4
The present embodiment cultivates Radix Astragali Auricularia.
The present embodiment provides a kind of Radix Astragali culture medium of edible fungus, including the raw material of following weight portion：
50 parts of Radix Astragali leftover bits and pieces, 330 parts of birch bits, 80 parts of Testa Tritici, 10 parts of Semen Glycines powderes, 2 parts of calcium lime powders, 150 parts of cotton seed hullss.
The method for carrying out Radix Astragali fungus cultivation using the Radix Astragali culture medium of edible fungus, comprises the following steps：
Make Radix Astragali Auricularia parent species：Take respectively 5 parts of Radix Astragali leftover bits and pieces, 25 parts of Semen Maydis powder, 2 parts of glucoses, 25 parts of agar, 1
Part magnesium sulfate, 0.1 part of potassium dihydrogen phosphate, add 1000 parts of water, and loading culture test tube enters pressure cooker and sterilizes after being sufficiently mixed uniformly,
Pressure 0.16MPa, temperature is incubated 40 minutes for 126 DEG C, after the completion of sterilizing, obtains Radix Astragali mother culture media, treats that kettle temperature drops
To 60 DEG C, the Radix Astragali mother culture media is removed into pressure cooker, temperature is down to 20 DEG C, the Radix Astragali mother culture media is moved into into nothing
Bacterium clean room；
Auricularia parent species are inoculated into into the Radix Astragali mother culture media in sterile purification room, are 65%, 24 DEG C of constant temperature in humidity
Culture 48 hours, culture culture in 12 days is continued afterwards at 25 DEG C and obtains Radix Astragali Auricularia parent species；
(2) Radix Astragali Auricularia original seed is made：The Radix Astragali is taken respectively according to the Radix Astragali culture medium of edible fungus parts by weight of raw materials
Leftover bits and pieces, wood flour (crushing 50 mesh sieves), Testa Tritici, Semen Glycines powder and calcium lime powder are dry-mixed 30 minutes, obtain mixed dry material；Mix to described
Close and 1155 parts of water (amount of water accounts for the 65% of mixing wet feed gross weight) are added in siccative, wet mixing 20 minutes, adjusting pH is after 7.6,
Obtain mixing wet feed, often cultivate the packed mixing wet feed 2.2kg, then culture bag is moved into into autoclave, be incubated at 100 DEG C
Carry out normal-pressure sterilization within 8 hours, obtain the Radix Astragali culture medium of edible fungus, kettle temperature is down to 70 DEG C, by Radix Astragali edible fungi culture
Base removes autoclave, and temperature is down to 26 DEG C, and Radix Astragali culture medium of edible fungus is moved into into sterile purification room；
It is female per culture bag Radix Astragali culture medium of edible fungus inoculation 4mL steps (1) Radix Astragali Auricularia in sterile purification room
Kind, aseptic culture is carried out afterwards 17 days, obtain Radix Astragali Auricularia original seed；The humidity of the aseptic culture is 70%, the aseptic training
Foster temperature control is as follows：At 25 DEG C, temperature is down to 24 DEG C to front 3 days temperature controls within 4-10 days, and temperature is down to 22 DEG C within 11-14 days,
15-17 is cooled to 10 DEG C with the rate of temperature fall of 4 DEG C/day；
(3) ear is gone out：The obtained culture bag equipped with the Radix Astragali culture medium of edible fungus of step (2) is taken again, and is moved into aseptic
Clean room, the Radix Astragali Auricularia original seed described in inoculation 20mL steps (2) per culture bag is cultivated 20 hours strains at 25 DEG C and is sprouted,
Enter back into sterile purification and educate bacterium room and be maintained at 25 DEG C, aseptic culture 30 days.Afterwards keeping temperature is 20 DEG C, and in good time water spray ensures wet
Spend for 85%, carry out urging ear, ear footpath is 1-2cm after 30 days, move to it is aseptic go out ear room carry out out ear training orientation, control training on daytime
Foster temperature is 25 DEG C, and night temperatures are 15 DEG C, and in good time water spray ensures that humidity is 90-95%, and winter is controlled with solar energy sunshade plate
Intensity of illumination 1500Lx, daily illumination 8 hours, it is ensured that 4 times/day of ventilation number, 30 minutes every time；I.e. when auricle is sufficiently spread out,
The basal part of the ear attenuates, and can harvest when about medium well, always collects three batches, goes out the ear cycle for 70 days.
After testing, the present embodiment cultivation obtains the sporophore diameter 6-15cm of Radix Astragali Auricularia, fresh ear yield：1.72kg/
Kg siccatives, compared with Radix Astragali yield is not added with culture medium 28.4% is increased, and total amino acid content accounting (is detected) after drying：12.98%, must
Aminoacid is needed to account for total amino acidss amount：38.2%.
Embodiment 5
The present embodiment cultivates Radix Astragali Hericium erinaceus (Bull. Ex Fr.) Pers..
The present embodiment provides a kind of Radix Astragali culture medium of edible fungus, including the raw material of following weight portion：
30 parts of Radix Astragali leftover bits and pieces, 220 parts squeeze wood flour, 100 parts of Radix Astragali stalks, 60 parts of Testa Tritici, 15 parts of Semen Glycines powderes, 2 parts of calcium lime powders,
5 parts of Gypsum Fibrosum, 80 parts of cotton seed hullss.
The method for carrying out Radix Astragali Hericium erinaceus culture using the Radix Astragali culture medium of edible fungus, comprises the following steps：
(3) Radix Astragali Hericium erinaceus (Bull. Ex Fr.) Pers. parent species are made：5 parts of Radix Astragali leftover bits and pieces, 25 parts of Semen Maydis powder, 3 parts of glucoses, 25 parts of fine jades are taken respectively
Fat, 1 part of potassium dihydrogen phosphate, add 1000 parts of water, are sufficiently mixed the uniform rear culture test tube that loads and enter pressure cooker sterilizing, pressure
0.16MPa, temperature is incubated 40 minutes for 126 DEG C, after the completion of sterilizing, obtains Radix Astragali mother culture media, treats that kettle temperature is down to 70
DEG C, the Radix Astragali mother culture media is removed into pressure cooker, temperature is down to 28 DEG C, the Radix Astragali mother culture media is moved into aseptic net
Change room；
Hericium erinaceus (Bull. Ex Fr.) Pers. parent species are inoculated into into the Radix Astragali mother culture media in sterile purification room, are 65% in humidity, constant temperature 24
Piece of tissue surrounding starts to grow the new mycelia of radial white after DEG C culture 48 hours, continues to cultivate 13 days at 25 DEG C afterwards and cultivates
Obtain Radix Astragali edible fungi parent species；
(2) Radix Astragali Hericium erinaceus (Bull. Ex Fr.) Pers. original seed is made：The Huang is taken respectively according to the Radix Astragali culture medium of edible fungus parts by weight of raw materials
Stilbene leftover bits and pieces, wood flour (crushing 50 mesh sieves), Testa Tritici, Semen Glycines powder and calcium lime powder are dry-mixed 30 minutes, obtain mixed dry material；To described
1088 parts of water (amount of water accounts for the 68% of mixing wet feed gross weight), wet mixing 20 minutes are added to adjust pH for 7.6 in mixed dry material
Afterwards, obtain mixing wet feed, often cultivate the packed mixing wet feed 2.2kg, then culture bag is moved into into autoclave, at 100 DEG C
Insulation carries out normal-pressure sterilization in 10 hours, obtains the Radix Astragali culture medium of edible fungus, and kettle temperature is down to 70 DEG C, by Radix Astragali edible fungi
Culture medium removes autoclave, and temperature is down to 28 DEG C, and Radix Astragali culture medium of edible fungus is moved into into sterile purification room；
It is female per culture bag Radix Astragali culture medium of edible fungus inoculation 5mL steps (1) Radix Astragali Hericium erinaceus (Bull. Ex Fr.) Pers. in sterile purification room
Kind, aseptic culture is carried out afterwards 17 days, obtain Radix Astragali Hericium erinaceus (Bull. Ex Fr.) Pers. original seed；The humidity of the aseptic culture is 70%, described aseptic
The temperature control of culture is as follows：At 25 DEG C, temperature is down to 24 DEG C to front 3 days temperature controls within 4-10 days, and temperature is down to 22 within 11-15 days
DEG C, the rate of temperature fall with 6 DEG C/day is cooled to 10 DEG C within 16-17 days；
(3) fruiting：The obtained culture bag equipped with the Radix Astragali culture medium of edible fungus of step (2) is taken again, and is moved into aseptic
Clean room, the Radix Astragali Hericium erinaceus (Bull. Ex Fr.) Pers. original seed described in inoculation 15mL steps (2) per culture bag, cultivates 24 hours strains and sprouts at 30 DEG C
Send out, enter back into sterile purification and educate bacterium room and be maintained at 26 DEG C, aseptic culture after 35 days mycelia enter physiological maturity, into it is aseptic go out
Mushroom room, carries out mushroom producing culture management, and it is 22 DEG C to control cultivation temperature on daytime, and night temperatures are 18 DEG C, humidity 85%, and winter is with too
Sun energy sunshading board control intensity of illumination 800Lx, daily light application time is 6 hours, and 13 days long fruiting flower buds continue culture 27 days, i.e.,
First batch of Radix Astragali Hericium erinaceus (Bull. Ex Fr.) Pers. can be harvested, two batches are always collected, the whole mushroom producing culture cycle is 60 days.
After testing, the present embodiment cultivation obtains the mushroom lid diameter of Radix Astragali Hericium erinaceus (Bull. Ex Fr.) Pers.：16-20cm, equal fresh mushroom production：
1.79kg/kg siccatives, compared with Radix Astragali yield is not added with culture medium 14.0% is increased, and total amino acid content accounting (is detected) after drying：
22.24%, it is necessary to which aminoacid accounts for total amino acidss amount：38.72%.
Embodiment 6
The present embodiment cultivates Radix Astragali Pleurotus ostreatus.
The present embodiment provides a kind of Radix Astragali culture medium of edible fungus, including the raw material of following weight portion：
50 parts of Radix Astragali leftover bits and pieces, 300 parts of Radix Astragali stalks, 60 parts of Testa Tritici, 15 parts of Semen Glycines powderes, 2 parts of calcium lime powders, 0.1 part of Gypsum Fibrosum,
180 parts of cotton seed hullss.
The method for carrying out Radix Astragali mushroom cultivation using the Radix Astragali culture medium of edible fungus, comprises the following steps：
(1) Radix Astragali Pleurotus ostreatus parent species are made：5 parts of Radix Astragali leftover bits and pieces, 25 parts of Semen Maydis powder, 10 parts of glucoses, 20 parts of fine jades are taken respectively
Fat, 1 part of magnesium sulfate, 1 part of potassium dihydrogen phosphate, add 950 parts of water, and loading culture test tube enters pressure cooker and goes out after being sufficiently mixed uniformly
Bacterium, pressure 0.15MPa, temperature is incubated 50 minutes for 125 DEG C, after the completion of sterilizing, obtains Radix Astragali mother culture media, treats kettle temperature
70 DEG C are down to, the Radix Astragali mother culture media is removed into pressure cooker, temperature is down to 22 DEG C, the Radix Astragali mother culture media is moved into
Sterile purification room；
Pleurotus ostreatus parent species are inoculated into into the Radix Astragali mother culture media in sterile purification room, are 60% in humidity, 24 DEG C of constant temperature
Piece of tissue surrounding starts to grow the new mycelia of radial white after cultivating 48 hours, continues to cultivate at 25 DEG C afterwards and cultivates for 11 days
To Radix Astragali Pleurotus ostreatus parent species；
(2) Radix Astragali Pleurotus ostreatus original seed is made：The Radix Astragali is taken respectively according to the Radix Astragali culture medium of edible fungus parts by weight of raw materials
Leftover bits and pieces, wood flour, Testa Tritici, Semen Glycines powder and calcium lime powder are dry-mixed 25 minutes, obtain mixed dry material；Add in the mixed dry material
1416 parts of water (amount of water accounts for the 70% of mixing wet feed gross weight), wet mixing 25 minutes, it is after 7, to obtain mixing wet feed, often to adjust pH
The packed mixing wet feed 2.2kg is cultivated, then culture bag autoclave is moved into into, the insulation at 100 DEG C carries out normal pressure for 9 hours and goes out
Bacterium, obtains the Radix Astragali culture medium of edible fungus, and kettle temperature is down to 70 DEG C, and Radix Astragali culture medium of edible fungus is removed into autoclave, temperature
Degree is down to 22 DEG C, and Radix Astragali culture medium of edible fungus is moved into into sterile purification room；
In sterile purification room, per culture bag Radix Astragali culture medium of edible fungus inoculation 5mL steps (1) Radix Astragali Pleurotus ostreatus parent species
Inoculation, carries out afterwards aseptic culture 17 days, obtains Radix Astragali Lentinus Edodess original seed；The humidity of the aseptic culture is 70%, described aseptic
The temperature control of culture is as follows：At 25 DEG C, temperature is down to 24 DEG C to front 3 days temperature controls within 4-10 days, and temperature is down to 22 within 11-15 days
DEG C, 16-17 is cooled to 10 DEG C with the rate of temperature fall of 6 DEG C/day；
(3) fruiting：The obtained culture bag equipped with the Radix Astragali culture medium of edible fungus of step (2) is taken again, and is moved into aseptic
Clean room, the Radix Astragali Pleurotus ostreatus original seed described in inoculation 15mL steps (2) per culture bag is cultivated 24 hours strains at 28 DEG C and is sprouted,
Enter back into sterile purification and educate bacterium room and be maintained at 25 DEG C, aseptic culture after 35 days mycelia enter physiological maturity, into aseptic fruiting
Room, carries out mushroom producing culture management, and it is 24 DEG C to control cultivation temperature on daytime, and night temperatures are 14 DEG C, humidity 95%, the winter sun
Energy sunshading board control intensity of illumination 1000Lx, daily light application time is 2 hours, and 15 days long fruiting flower buds continue to cultivate 5 days, you can
First batch of Radix Astragali Pleurotus ostreatus is plucked, three batches are always collected, the whole mushroom producing culture cycle is 30 days.
After testing, the present embodiment cultivation obtains the mushroom lid diameter 7-11cm of Radix Astragali Pleurotus ostreatus, equal fresh mushroom production：1.84kg/kg
Siccative, compared with Radix Astragali yield is not added with culture medium 22.7% is increased, and total amino acid content accounting (is detected) after drying：24.03%, it is necessary to
Aminoacid accounts for total amino acidss amount：39.83%.
Embodiment 7
The present embodiment cultivates Radix Astragali Pleurotus eryngii.
The present embodiment provides a kind of Radix Astragali culture medium of edible fungus, including the raw material of following weight portion：
50 parts of Radix Astragali leftover bits and pieces, 180 parts squeeze wood flour, 150 parts of birch bits, 80 parts of Testa Tritici, 15 parts of Semen Glycines powderes, 2 parts of calcium lime powders,
2 parts of Gypsum Fibrosum.
The method for carrying out Radix Astragali planting almond abalone mushroom using the Radix Astragali culture medium of edible fungus, comprises the following steps：(1) make
Radix Astragali Pleurotus eryngii parent species：Take respectively 5 parts of Radix Astragali leftover bits and pieces, 25 parts of Semen Maydis powder, 10 parts of glucoses, 20 parts of agar, 1 part of magnesium sulfate, 2
Part potassium dihydrogen phosphate, adds 920 parts of water, and loading culture test tube enters pressure cooker and sterilizes after being sufficiently mixed uniformly, pressure 0.15MPa,
Temperature is incubated 50 minutes for 126 DEG C, after the completion of sterilizing, obtains Radix Astragali mother culture media, treats that kettle temperature is down to 70 DEG C, will be described
Radix Astragali mother culture media removes pressure cooker, and temperature is down to 24 DEG C, and the Radix Astragali mother culture media is moved into into sterile purification room；
Pleurotus eryngii parent species are inoculated into into the Radix Astragali mother culture media in sterile purification room, are 70% in humidity, constant temperature 24
Piece of tissue surrounding starts to grow the new mycelia of radial white after DEG C culture 48 hours, continues to cultivate 12 days at 25 DEG C afterwards and cultivates
Obtain Radix Astragali Pleurotus eryngii parent species；
(2) Radix Astragali Pleurotus eryngii original seed is made：The Huang is taken respectively according to the Radix Astragali culture medium of edible fungus parts by weight of raw materials
Stilbene leftover bits and pieces, wood flour, Testa Tritici, Semen Glycines powder and calcium lime powder are dry-mixed 25 minutes, obtain mixed dry material；Add in the mixed dry material
720 parts of water (amount of water accounts for the 60% of mixing wet feed gross weight), wet mixing 25 minutes, it is after 7.2, to obtain mixing wet feed to adjust pH,
The packed mixing wet feed 2.2kg is often cultivated, then culture bag autoclave is moved into into, the insulation at 102 DEG C carries out normal pressure in 8 hours
Sterilizing, obtains the Radix Astragali culture medium of edible fungus, and kettle temperature is down to 70 DEG C, and Radix Astragali culture medium of edible fungus is removed into autoclave,
Temperature is down to 24 DEG C, and Radix Astragali culture medium of edible fungus is moved into into sterile purification room；
It is female per culture bag Radix Astragali culture medium of edible fungus inoculation 4mL steps (1) Radix Astragali Pleurotus eryngii in sterile purification room
Kind, aseptic culture is carried out afterwards 15 days, obtain Radix Astragali Pleurotus eryngii original seed；The humidity of the aseptic culture is 70%, described aseptic
The temperature control of culture is as follows：At 25 DEG C, temperature is down to 24 DEG C to front 3 days temperature controls within 4-10 days, the 11-15 days drops with 3 DEG C/day
Warm speed is cooled to 9 DEG C；
(3) fruiting：The obtained culture bag equipped with the Radix Astragali culture medium of edible fungus of step (2) is taken again, and is moved into aseptic
Clean room, the Radix Astragali Pleurotus eryngii original seed described in inoculation 20mL steps (2) per culture bag, cultivates 24 hours strains and sprouts at 30 DEG C
Send out, enter back into sterile purification and educate bacterium room and be maintained at 25 DEG C, aseptic culture after 33 days mycelia enter physiological maturity, into it is aseptic go out
Mushroom room, carries out mushroom producing culture management, and round the clock cultivation temperature is 10 DEG C for control, humidity 92%, and winter is with solar energy sunshade plate control
Intensity of illumination 500Lx processed, daily light application time is 4 hours, 60 days long fruiting flower buds, continues to cultivate 6 days, you can pluck first batch of Huang
Stilbene Pleurotus eryngii, always collects three batches, and the whole mushroom producing culture cycle is 65 days.
After testing, the mushroom lid diameter of the Radix Astragali Pleurotus eryngii that the present embodiment cultivation is obtained：5-8cm, equal fresh mushroom production：
1.85kg/kg siccatives, compared with Radix Astragali yield is not added with culture medium 32.1% is increased, and total amino acid content accounting (is detected) after drying：
23.10%, it is necessary to which aminoacid accounts for total amino acidss amount：39.23%.
The above, the only specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, all should contain
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be defined by the scope of the claims.
Claims (10)
1. a kind of Radix Astragali culture medium of edible fungus, it is characterised in that including the raw material of following weight portion：30-70 part Radix Astragali leftover bits and pieces,
280-350 parts wood flour and/or Radix Astragali stalk, 50-100 part Testa Tritici, 5-20 part Semen Glycines powderes, 2-8 part calcium lime powders.
2. Radix Astragali culture medium of edible fungus according to claim 1, it is characterised in that the wood flour is to squeeze wood flour, birch bits
Or one or more in basswood bits.
3. Radix Astragali culture medium of edible fungus according to claim 1, it is characterised in that also including 0.1-5 part Gypsum Fibrosum.
4. Radix Astragali culture medium of edible fungus according to claim 1, it is characterised in that also including 80-180 part cotton seed hullss.
5. the method for carrying out Radix Astragali edible fungus culturing using the Radix Astragali culture medium of edible fungus described in any one of claim 1-4, its
It is characterised by, comprises the following steps：
(1) Radix Astragali edible fungi parent species are made：Take respectively 2-8 part Radix Astragali leftover bits and pieces, 20-30 part Semen Maydis powder, 2-20 part glucoses,
20-30 part agar, adds 900-1000 part water, be sufficiently mixed it is uniform, it is sterilized after, obtain Radix Astragali mother culture media；
Take parent edible fungus kind and be inoculated into the Radix Astragali mother culture media, afterwards temperature be 24-26 DEG C, humidity be 60-70%
Under the conditions of cultivated, obtain Radix Astragali edible fungi parent species；
(2) Radix Astragali edible fungi original seed is made：According to the weight portion take respectively the Radix Astragali leftover bits and pieces, wood flour and/or the Radix Astragali stalk,
Testa Tritici, Semen Glycines powder and calcium lime powder, are sufficiently mixed uniformly, obtain mixed dry material；Adding water in the mixed dry material, it is wet to obtain mixing
Material, amount of water is the 55-75% of the mixing wet feed weight, and regulation pH value is 7-8, by the mixing wet feed sterilizing, obtains final product institute
State Radix Astragali culture medium of edible fungus；
Step (1) Radix Astragali edible fungi parent species are inoculated in the Radix Astragali culture medium of edible fungus, aseptic culture is carried out afterwards
After 15-20 days, Radix Astragali edible fungi original seed is obtained；The humidity of the aseptic culture be 60-70%, the temperature control of the aseptic culture
System is as follows：At 25 DEG C, temperature is down to 23-24 DEG C to front 3 days temperature controls within 4-10 days, drops to 8-22 DEG C within 11-20 days；
(3) bacterium is gone out：Radix Astragali edible fungi original seed described in Radix Astragali culture medium of edible fungus inoculation step (2) obtained in step (2) is taken again,
Carry out mycelia after mycelia culture 30-40 days and enter physiological maturity, then carry out out bacterium training orientation, that is, complete the Radix Astragali food
With the cultivation of bacterium.
6. the method for Radix Astragali edible fungus culturing according to claim 5, it is characterised in that in step (1), the Radix Astragali is female
The raw material components for planting culture medium also add 0.1-1 parts magnesium sulfate and 0.1-2 part potassium dihydrogen phosphates.
7. the method for Radix Astragali edible fungus culturing according to claim 5, it is characterised in that in step (1), the sterilizing
Pressure is 0.15-0.18MPa, and the temperature of the sterilizing is 120-130 DEG C, and the time of the sterilizing is 30-60 minutes.
8. the method for Radix Astragali edible fungus culturing according to claim 5, it is characterised in that in step (2), the sterilizing is adopted
With normal-pressure sterilization, the temperature of the sterilizing is 100-107 DEG C, and the time of the sterilizing is 8-14 hours.
9. the method for Radix Astragali edible fungus culturing according to claim 5, it is characterised in that in step (3), the mycelia training
Foster temperature be 25-32 DEG C, it is described go out bacterium culture temperature be 10-25 DEG C, air humidity is 85-95%, and intensity of illumination is 50-
3500Lx。
10. the method for Radix Astragali edible fungus culturing according to claim 5, it is characterised in that in step (3), is carried out described
The light application time for going out bacterium training orientation is controlled to 2-8 hours/day.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107509529A (en) * | 2017-08-23 | 2017-12-26 | 佛山推启农业研究院（普通合伙） | A kind of artificial cultivation method for improving hickory chick amino acid content |
| CN107805098A (en) * | 2017-10-31 | 2018-03-16 | 贵州省印江自治县梵净山生态菌业有限公司 | A kind of culture matrix of mushroom and preparation method thereof |
| CN107926483A (en) * | 2017-12-26 | 2018-04-20 | 张掖金泰现代农业科技有限责任公司 | A kind of cultivation compost of radix astragali mushroom and the preparation method of radix astragali mushroom |
| CN108307928A (en) * | 2018-02-27 | 2018-07-24 | 尤溪九牧林生物科技有限公司 | A kind of linden clinker cultivation mushroom method |
| CN111052983A (en) * | 2018-10-16 | 2020-04-24 | 郭立潮 | Astragalus membranaceus mushroom culture medium |
| CN111802174A (en) * | 2020-07-07 | 2020-10-23 | 南京晓庄学院 | Compost for improving amino acid content of hericium erinaceus, preparation method and application |
| CN113875494A (en) * | 2021-10-19 | 2022-01-04 | 山西裕隆祥农业发展有限公司 | Method for culturing functional shiitake mushrooms capable of resisting cancer cell proliferation and regulating tumor immunity |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078345A (en) * | 1993-03-18 | 1993-11-17 | 陈玉春 | The breeding method of membranous milk vetch mushroom |
-
2017
- 2017-01-04 CN CN201710004724.7A patent/CN106673851A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078345A (en) * | 1993-03-18 | 1993-11-17 | 陈玉春 | The breeding method of membranous milk vetch mushroom |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107509529A (en) * | 2017-08-23 | 2017-12-26 | 佛山推启农业研究院（普通合伙） | A kind of artificial cultivation method for improving hickory chick amino acid content |
| CN107805098A (en) * | 2017-10-31 | 2018-03-16 | 贵州省印江自治县梵净山生态菌业有限公司 | A kind of culture matrix of mushroom and preparation method thereof |
| CN107926483A (en) * | 2017-12-26 | 2018-04-20 | 张掖金泰现代农业科技有限责任公司 | A kind of cultivation compost of radix astragali mushroom and the preparation method of radix astragali mushroom |
| CN108307928A (en) * | 2018-02-27 | 2018-07-24 | 尤溪九牧林生物科技有限公司 | A kind of linden clinker cultivation mushroom method |
| CN111052983A (en) * | 2018-10-16 | 2020-04-24 | 郭立潮 | Astragalus membranaceus mushroom culture medium |
| CN111802174A (en) * | 2020-07-07 | 2020-10-23 | 南京晓庄学院 | Compost for improving amino acid content of hericium erinaceus, preparation method and application |
| CN113875494A (en) * | 2021-10-19 | 2022-01-04 | 山西裕隆祥农业发展有限公司 | Method for culturing functional shiitake mushrooms capable of resisting cancer cell proliferation and regulating tumor immunity |
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