CN107926483A - A kind of cultivation compost of radix astragali mushroom and the preparation method of radix astragali mushroom - Google Patents
A kind of cultivation compost of radix astragali mushroom and the preparation method of radix astragali mushroom Download PDFInfo
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- CN107926483A CN107926483A CN201711426896.XA CN201711426896A CN107926483A CN 107926483 A CN107926483 A CN 107926483A CN 201711426896 A CN201711426896 A CN 201711426896A CN 107926483 A CN107926483 A CN 107926483A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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Abstract
A kind of cultivation compost the present invention provides radix astragali mushroom and the radix astragali mushroom cultivating method using the compost.The present invention is used as the major ingredient of cultivation compost using radix astragali stalk and radix astragali stilbene head, with maize straw, corncob etc. for auxiliary material, cost can be effectively reduced by using discarded radix astragali stalk and stilbene head, and biological efficiency can be effectively improved, contain the materials such as astragalus polyose, saponin(e, flavones in radix astragali mushroom at the same time, the nutritive value of mushroom is improved, and makes peculiar taste of the mushroom with radix astragali, it is more fresh and tender tasty and refreshing.
Description
Technical field
The invention belongs to Edible Fungi field, and in particular to the cultivation compost and radix astragali mushroom of a kind of radix astragali mushroom
Preparation method.
Background technology
Gansu is the important Chinese medicine original producton location in China and main product, and cultivated area is big, and yield is high, the hair of Chinese herbal medicine industry
Exhibition have accumulated substantial amounts of radix astragali stalk and radix astragali stilbene head, and the processing mode to radix astragali stalk and radix astragali stilbene head is discarding or combustion at present
Burn, and environmental pollution and the wasting of resources can be caused by arbitrarily stacking and burning.
The content of the invention
It is an object of the invention to provide a kind of method using the first-born production radix astragali mushroom of radix astragali stalk and radix astragali stilbene, solve
Radix astragali stalk and radix astragali stilbene head is arbitrarily stacked and burning produces problem of environmental pollution and radix astragali stalk and radix astragali stilbene head recycling
Utilizing question.In order to realize foregoing invention purpose, the present invention provides following technical scheme：
The present invention provides a kind of cultivation compost of radix astragali mushroom, include the component of following parts by weight：
Radix astragali stalk and radix astragali stilbene is 30~40 parts first, maize straw and 20~30 parts of corncob, 10~20 parts of cotton seed hulls, wood
Bits 20~30 parts, 10~15 parts of wheat bran, 2~3 parts of corn flour, 2~3 parts of analysis for soybean powder, 0.5~1 part of gypsum, 0.5~1 part of lime,
0.05~0.15 part of dipotassium hydrogen phosphate, 0.05~0.15 part of potassium dihydrogen phosphate, 0.05~0.15 part of magnesium sulfate, material-water ratio 1:
1.25~1.50；
The weight ratio of radix astragali stalk and radix astragali the stilbene head is 1:0.9~1.1；The weight of the maize straw and corncob
Than for 1:0.9~1.1.
Present invention also offers it is a kind of containing described in above-mentioned technical proposal cultivate compost radix astragali mushroom cultivating method,
Comprise the following steps：
1) it is inoculated with when sterilizing by the material bag equipped with compost, be cooled to less than 25 DEG C, obtains lentinus edodes strain stick；
2) the lentinus edodes strain stick bacterium germination for obtaining the step 1), bag is taken off after mycelial growth purseful, and solid stacks to obtain annesl
Lentinus edodes strain stick；
3) by annesl lentinus edodes strain stick flower bud that the step 2) obtains, grow to obtain radix astragali mushroom.
Preferably, the preparation method of inoculation strain used comprises the following steps in the step 1)：
A. the dicaryon mycelium of mushroom is inoculated in PDA culture medium and cultivates to obtain mycelium, the mycelium is transferred to mixed
Synthase liquid digests, and obtains enzymolysis liquid；The mixed enzymolysis liquid includes lywallzyme, cellulase and glusulase；
B. the obtained enzymolysis liquids of the step a are diluted successively, filtered, centrifuged, taken middle level liquid to purify protoplast and hang
Liquid；
C. the purification protoplast suspension step b obtained is cultivated in PDA nutrient solutions, obtains Protoplast cuhnre
Liquid；
D. the Protoplast cuhnre liquid step c obtained uses gradient dilution method, after applying 8~12d of PDA plate culture
Tube culture, the mycelium that picking single bacterium colony is sprouted, carries out microexamination, obtains the monocaryon X of no clamp connection and have
The dicaryon Y of clamp connection, distinguishes tube culture by X, Y；
E. the obtained monocaryon X of the step d are only mated, that is, takes 2 monocaryon X, at a distance of 0.4~0.6cm
It is inoculated in comprehensive PDA culture medium and cultivates, the mycelium of two bacterium colony laps is taken after two bacterium colonies intersect 5~7d, is gone to comprehensive
Close and more than 3d is cultivated in PDA culture medium, obtain the nucleated mycelium with clamp connection, expand culture；
F. the nucleated mycelium step d obtained dicaryon Y or step e obtained is as strain.
Preferably, by mass percentage, the total concentration of mixed enzyme solution is 0.9~1.1% in the step a.
Preferably, lywallzyme in mixed enzyme solution in the step a：Cellulase：The weight ratio of glusulase is 1.9~2.1:
0.9~1.1:0.9~1.1.
Preferably, the step f is further included after obtaining strain：The strain liquid culture that will be obtained in step f, obtains liquid
Strain.
Preferably, the preparation method of the liquid spawn comprises the following steps：
(1) strain that the inoculation step f is obtained is inoculated into culture medium and carries out liquid fermentation culture, obtains mushroom liquid bacteria
Kind parent species；
(2) the mushroom liquid bacterial parent species that the step (1) obtains are connected in fermentation medium, carry out liquid fermentation training
Support to obtain liquid spawn.
Preferably, the culture medium of liquid fermentation and culture includes the component of following mass percentage in the step (1)：
Corn flour 1.0%~3.0%, analysis for soybean powder 1.0%~2.0%, yeast extract 0.1%~0.2%, dipotassium hydrogen phosphate
0.05%~0.15%, potassium dihydrogen phosphate 0.05%~0.15%, magnesium sulfate 0.05%~0.15% and surplus are water；
Fermentation medium includes the component of following mass percentage in the step (2)：
Wheat bran 2%~5%, corn flour 1%~3%, analysis for soybean powder 1~2%, dipotassium hydrogen phosphate 0.05%~0.15%, phosphorus
Acid dihydride potassium 0.05%~0.15%, magnesium sulfate 0.05%~0.10% and surplus are water.
Preferably, the condition in the step 2) during bacterium germination is：20~25 DEG C of temperature, humidity 65~75%, daily ventilation
0.5~1.5 it is small when, inoculation 5~7 days after, every turning in 8~10 days once.
Preferably, the condition of flower bud is in the step 3)：10~22 DEG C of temperature, 5~10 DEG C of day and night temperature, air humidity
85~95%；
The condition of growth is in the step 3)：8~25 DEG C of temperature, air humidity 85~90%, ventilation 1~5 is small daily
When.
The cultivation side of radix astragali mushroom the present invention provides a kind of cultivation compost of radix astragali mushroom and using the compost
Method, the present invention is using radix astragali stalk and radix astragali stilbene head as the major ingredient of cultivation compost, with maize straw, corncob etc. for auxiliary material,
The radix astragali mushroom for making to be prepared reaches following effect：
1. reduce cost：Cost, every 1.0 jin of siccatives can be effectively reduced by using discarded radix astragali stalk and stilbene head
Cost can be reduced by more than 0.36 yuan.
2. improve biological efficiency：Test result indicates that produce every bag of produce surviving of son entity 6~7 of mushroom using the method for the present invention
Two, 5~6 liang of the produce surviving of son entity of every bag of compost based on solid spawn cotton seed hulls, improves 10%~20%.
3. improve the nutritive value of mushroom：Contain the materials such as astragalus polyose, saponin(e, flavones in radix astragali mushroom.
4. mushroom flavor is more preferably, the mushroom cultivated with radix astragali stalk and stilbene head carries the peculiar taste of radix astragali, more fresh
It is tender tasty and refreshing.
Embodiment
The present invention provides a kind of cultivation compost of radix astragali mushroom, include the component of following parts by weight：
Radix astragali stalk and radix astragali stilbene is 30~40 parts first, maize straw and 20~30 parts of corncob, 10~20 parts of cotton seed hulls, wood
Bits 20~30 parts, 10~15 parts of wheat bran, 2~3 parts of corn flour, 2~3 parts of analysis for soybean powder, 0.5~1 part of gypsum, 0.5~1 part of lime,
0.05~0.15 part of dipotassium hydrogen phosphate, 0.05~0.15 part of potassium dihydrogen phosphate, 0.05~0.15 part of magnesium sulfate, material-water ratio 1:
1.25~1.50；
The weight ratio of radix astragali stalk and radix astragali the stilbene head is 1:0.9~1.1；The weight of the maize straw and corncob
Than for 1:0.9~1.1.
In the present invention, the cultivation compost of the radix astragali mushroom preferably includes the component of following parts by weight：Radix astragali stalk
23~27 parts of 33~37 parts first with radix astragali stilbene, maize straw and corncob, 13~17 parts of cotton seed hulls, 23~27 parts of sawdust, wheat bran
12~14 parts, 2.3~2.7 parts of corn flour, 2.3~2.7 parts of analysis for soybean powder, 0.6~0.8 part of gypsum, 0.6~0.8 part of lime, phosphoric acid
0.08~0.12 part of hydrogen dipotassium, 0.08~0.12 part of potassium dihydrogen phosphate, 0.08~0.12 part of magnesium sulfate, material-water ratio 1:1.35~
1.45；More preferably include：Radix astragali stalk and radix astragali stilbene is 35 parts first, maize straw and 25 parts of corncob, 15 parts of cotton seed hulls, sawdust 25
Part, 13 parts of wheat bran, 2.5 parts of corn flour, 2.5 parts of analysis for soybean powder, 0.7 part of gypsum, 0.7 part of lime, 0.10 part of dipotassium hydrogen phosphate, phosphoric acid
0.10 part of potassium dihydrogen, 0.10 part of magnesium sulfate, material-water ratio 1:1.4.
In the present invention, the weight ratio of radix astragali stalk and radix astragali the stilbene head is preferably 1:0.95~1.05, more preferably
1:1.The weight ratio of the maize straw and corncob is preferably 1:0.95~1.05, more preferably 1:1.
The source of the component of cultivation compost of the present invention to above-mentioned radix astragali mushroom is not particularly limited, using commercial goods
.
In the present invention, the preparation method of the cultivation compost is by the cultivation compost of the above-mentioned radix astragali mushroom provided
Raw material mixes in proportion.
Present invention also offers it is a kind of using described in above-mentioned technical proposal cultivate compost radix astragali mushroom cultivating method,
Comprise the following steps：
1) it is inoculated with when sterilizing by the material bag equipped with compost, be cooled to less than 25 DEG C, obtains lentinus edodes strain stick；
2) the lentinus edodes strain stick bacterium germination for obtaining the step 1), bag is taken off after mycelial growth purseful, and solid stacks to obtain annesl
Lentinus edodes strain stick；
3) by annesl lentinus edodes strain stick flower bud that the step 2) obtains, grow to obtain radix astragali mushroom.
In the present invention, the material bag equipped with compost is sterilized the material bag that must sterilize.Preferably, the sterilization method is normal pressure
Sterilize 24 it is small when.Preferably, the sterilising temp is makes in material bag temperature more than 90 DEG C.Preferably, the size of the material bag
It is most preferably 17cm × 48cm for 15~19cm × 45~50cm, more preferably 16~18cm × 46~49cm.Preferably, institute
The loadings for stating compost in material bag are 2.8Kg~3.2Kg, more preferably 2.9Kg~3.1Kg, are most preferably 3.0Kg.
After obtaining sterilizing material bag, the present invention is inoculated with when sterilizing material bag is cooled to less than 25 DEG C, obtains lentinus edodes strain stick.It is preferred that
, the type of cooling cools down for room temperature.Preferably, in cooling procedure, material bag is placed in turnover box, to reduce pollution
Rate.
In the present invention, it is preferred to, the strain used in the inoculation reaches 808 bacterial strain of edible mushroom research institute mushroom using day.
In the present invention, the inoculation preferably includes following steps with the preparation method of strain：
A. the dicaryon mycelium of mushroom is inoculated in PDA culture medium and cultivates to obtain mycelium, the mycelium is transferred to mixed
Synthase liquid digests, and obtains enzymolysis liquid；The mixed enzymolysis liquid includes lywallzyme, cellulase and glusulase；
B. the obtained enzymolysis liquids of the step a are diluted successively, filtered, centrifuged, taken middle level liquid to purify protoplast and hang
Liquid；
C. the purification protoplast suspension step b obtained is cultivated in PDA nutrient solutions, obtains Protoplast cuhnre
Liquid；
D. the Protoplast cuhnre liquid step c obtained uses gradient dilution method, after applying 8~12d of PDA plate culture
Tube culture, the mycelium that picking single bacterium colony is sprouted, carries out microexamination, obtains the monocaryon X of no clamp connection and have
The dicaryon Y of clamp connection, distinguishes tube culture by X, Y；
E. the monocaryon X that two step d are obtained only is mated, that is, takes 2 monocaryon X, at a distance of 0.4~0.6cm
It is inoculated on comprehensive PDA tablet and cultivates, the mycelium of two bacterium colony laps is taken after two bacterium colonies intersect 5~7d, goes to synthesis
More than 3d is cultivated in PDA plate, obtains the nucleated mycelium with clamp connection, expands culture；
F. the nucleated mycelium step d obtained dicaryon Y or step e obtained is as strain.
In the present invention, the dicaryon mycelium of mushroom is inoculated in PDA culture medium and cultivates to obtain mycelium.The PDA trainings
Foster base is：Peeled potatoes 200g, glucose 20g, agar 20g and water 1000ml.The condition of the culture is preferably：Temperature 20
~28 DEG C, 4~6 days time；More preferably 23~26 DEG C, 4.5~5.5 days time；Most preferably 25 DEG C, 5 days time.Culture knot
Mycelium covers with culture medium after beam.Preferably, the inoculation carries out in an aseptic environment.Preferably, the inoculum concentration of the inoculation
For the strain block of 5.0 × 5.0 × 2.0mm.
In the present invention, obtained mycelium is transferred in mixed enzyme solution and digested, obtain enzymolysis liquid.The mixed enzymolysis liquid bag
Include lywallzyme, cellulase and glusulase.Preferably, the volume ratio of the mycelia weight and mixed enzyme solution is 0.8~1.2g:
4.5~5.5ml, more preferably, 0.9~1.1g:4.8~5.2ml, is most preferably 1g:5.0ml.By weight percentage, it is described
The total concentration of mixed enzyme solution is preferably 0.9~1.1%, and more preferably 0.95~1.05%, it is most preferably 1.0%.Preferably, institute
State the MgSO that mixed enzyme solution uses 0.6M4.7H2O solution dilutes.Lywallzyme in the mixed enzyme solution：Cellulase：Glusulase
Weight ratio is preferably 1.9~2.1:0.9~1.1:0.9~1.1, more preferably 1.95~2.05:0.95~1.05:0.95~
1.05, it is most preferably 2:1:1.In the present invention, it is preferred to, the condition of the enzymolysis is：32~36 DEG C of temperature, 350~
450rpm shaking tables, 4~8h of time；More preferably：33~35 DEG C of temperature, 390~410rpm shaking tables, 5~7h of time；Most preferably
For：34 DEG C of temperature, 400rpm shaking tables, time 6h.
In the present invention, after obtaining enzymolysis liquid, the enzymolysis liquid is diluted successively, filter, is centrifuged, take middle level liquid to obtain
Purify protoplast suspension.Preferably, the diluent used that dilutes is MgSO4.7H2O solution, more preferably 0.5~
The MgSO of 0.7mol/L4.7H2O solution, is most preferably 0.6mol/L.Preferably, diluted concentration MgSO4.7H2The use of O solution
Measure as 1.5~2.5 times of enzymolysis liquid volume, more preferably 1.8~2.2 times, be most preferably 2 times.Preferably, it is described to take middle level liquid
Mode to be pipetted with sterilizing pipette.
In the present invention, the filtering preferably uses aperture G3The filtering of number sand core funnel.The condition of the centrifugation is preferably：
3000~4000r/min of rotating speed, 5~10min of time；More preferably 3300~3700r/min, 6~8min；Most preferably
3500r/min, 7min.
In the present invention, obtained purification protoplast suspension is added in PDA nutrient solutions and cultivated, obtain Protoplast cuhnre
Liquid.Preferably, the volume ratio of the purification protoplast suspension and PDA nutrient solutions is：1:3.5~4.5, more preferably 1:3.8
~4.2, it is most preferably 1:4.Preferably, the training method is quiescent culture.Preferably, the condition of culture is：Temperature 22
~25 DEG C, 46~50h of time；More preferably 22.5~24 DEG C, 47~49h；Most preferably 23 DEG C, 48h.
The source of the PDA culture medium and the component of PDA nutrient solutions is not particularly limited in the present invention, using commercially available business
Product.
In the present invention, the Protoplast cuhnre liquid is used into gradient dilution method, turned after applying 8~12d of PDA plate culture
Pipe culture, the mycelium that picking single bacterium colony is sprouted, carries out microexamination, obtains the monocaryon X of no clamp connection and with lock
The united dicaryon Y of shape, distinguishes tube culture by X, Y.During the tablet culture additive amount of Protoplast cuhnre liquid for 2~
10%, more preferably 4~6%, are most preferably 5%.The tablet condition of culture is preferably：22~25 DEG C, 8~12d；More preferably
For 22.5~24 DEG C, 9~11d；Most preferably 23 DEG C, 10d.Occur regeneration bacterium colony at this time, bacterium colony tube culture will be regenerated.It is described
Tube condition of culture is preferably 21~25 DEG C, 8~12d；More preferably 22~24 DEG C, 9~11d；Most preferably 23 DEG C, 10d.
In the present invention, obtain two monocaryon X are inoculated in the tablet of comprehensive PDA culture medium at a distance of 0.4~0.6cm
Upper culture.The comprehensive PDA culture medium is：Peeled potatoes 200g, glucose 20g, agar 20g, potassium dihydrogen phosphate 3g, sulfuric acid
Magnesium 1.5g, vitamin B1Content is the vitamin B of 10 ㎎/piece13 and water 1000ml.The training method is preferably dark is inverted
Culture.The condition of culture is preferably：22~25 DEG C, 5~7d；More preferably 22.5~24 DEG C, 5.5~6.5d；Most preferably
23 DEG C, 6d.Two bacterium are intersected at this time, and the mycelium of two bacterium colony laps is taken after two bacterium colonies intersect 5~7d, goes to comprehensive PDA
More than 3d is cultivated in tablet, obtains the nucleated mycelium with clamp connection, expands culture, is preserved.The expansion training method is excellent
Elect switching PDA Tube propagations as.The condition of culture is preferably 21~25 DEG C, 8~12d；More preferably 22~24 DEG C, 9~
11d；Most preferably 23 DEG C, 10d.
The source of the component of the comprehensive PDA culture medium is not particularly limited in the present invention, using commercial goods.
In the present invention, using obtained dicaryon Y and the obtained nucleated mycelium with clamp connection as strain.Obtain
Strain bio character stablize.
In the present invention, after strain being prepared, the present invention preferably by obtained strain be inoculated into fluid nutrient medium into
Row liquid fermentation and culture obtains mushroom liquid bacterial parent species.Preferably, the culture medium sterilizes before inoculation.The sterilizing
Condition is preferably pressure：0.13Mpa~0.14Mpa, time：25~30min.Preferably, the culture medium bag of the Liquid Culture
Include the component of following mass percentage：Corn flour 1.0%~3.0%, analysis for soybean powder 1.0%~2.0%, yeast extract 0.1%~
0.2%th, dipotassium hydrogen phosphate 0.05%~0.15%, potassium dihydrogen phosphate 0.05%~0.15%, magnesium sulfate 0.05%~0.15%
It is water with surplus, more preferably, corn flour 1.5%~2.5%, analysis for soybean powder 1.2%~1.8%, yeast extract 0.13%~
0.17%th, dipotassium hydrogen phosphate 0.08%~0.12%, potassium dihydrogen phosphate 0.08%~0.12%, magnesium sulfate 0.08%~0.12%
It is water with surplus, is most preferably corn flour 2.0%, analysis for soybean powder 1.5%, yeast extract 0.15%, dipotassium hydrogen phosphate 0.10%, phosphoric acid
Potassium dihydrogen 0.10%, magnesium sulfate 0.10% and surplus are water.The pH of herein described culture medium is natural.
Culture medium temperature after subject to sterilization is inoculated with again after being down to less than 30 DEG C.Preferably, the inoculum concentration of the inoculation
It is most preferably 5% for 2~10%, more preferably 4~6%.Preferably, the inoculation aseptically carries out.Preferably,
The culture is 45~50h of quiescent culture at 24~26 DEG C, is sprouted on mycelia liquid medium within, then with 135~145r/min
3.5~4.5d of constant-temperature shaking culture；Quiescent culture 48h at more preferably 25 DEG C, then with 140r/min constant-temperature shaking cultures 4d.
The present invention is not particularly limited the source of the component of the culture medium of aforesaid liquid culture, using people in the art
The medicine of culture medium is formulated conventionally in member.
After obtaining mushroom liquid bacterial parent species, obtained mushroom liquid bacterial parent species are connected to fermentation medium by the present invention
In, fermented and cultured obtains liquid spawn.Preferably, the fermentation medium includes the component of following mass percentage：Wheat bran 2%
~5%, corn flour 1%~3%, analysis for soybean powder 1~2%, dipotassium hydrogen phosphate 0.05%~0.15%, potassium dihydrogen phosphate 0.05%~
0.15%th, magnesium sulfate 0.05%~0.10% and surplus are water, more preferably wheat bran 3%~4%, corn flour 1.5%~
2.5%th, analysis for soybean powder 1.2~1.8%, dipotassium hydrogen phosphate 0.08%~0.12%, potassium dihydrogen phosphate 0.08%~0.12%, sulfuric acid
Magnesium 00.08%~0.12% and surplus are water, are most preferably wheat bran 3.5%, corn flour 2%, analysis for soybean powder 1.5%, phosphoric acid hydrogen two
Potassium 0.1%, potassium dihydrogen phosphate 0.10%, magnesium sulfate 0.07% and surplus are water.Preferably, the granularity of the wheat bran for 0.4~
0.8mm, more preferably 0.5~0.7mm, are most preferably 0.6mm.
Preferably, the inoculum concentration is that the volume ratio of strain and fermentation medium is 0.9~1.1:50, more preferably 0.95
~1.05:50, it is most preferably 1:50.Preferably the condition of the fermented and cultured is：23~27 DEG C of temperature, pressure 0.015~
0.025Mpa, 3~5d of time；More preferably：24~26 DEG C, 0.017~0.022Mpa of pressure, 3.5~4.5d of time of temperature；Most
Preferably：25 DEG C, pressure 0.02Mpa, time 4d of temperature.
Preferably, the present invention adds the defoamer that mass content is 0.1~0.2% in fermented and cultured, more preferably,
0.13~0.17%, it is most preferably 0.15%.
Being not particularly limited from kind to above-mentioned defoamer of the invention, using conventional commercial product.It is preferred that
, using 1,001 board formulated food additive bean product defoamers.
The present invention is not particularly limited the source of the component of above-mentioned fermentation medium, conventional using those skilled in the art
Prepare the medicine of culture medium.
It is inoculated with when sterilizing by the material bag equipped with compost, be cooled to less than 25 DEG C, obtains lentinus edodes strain stick.Preferably, it is described to connect
Kind carries out in the transfer room after sterilizing according to a conventional method.Preferably, the vaccination ways are in bacterium bag homonymy to make a call to three, with connecing
Kind rifle is inoculated with vaccination, is sealed after inoculation.Preferably, the inoculum concentration of the inoculation is inoculated with mushroom liquid bacteria for each vaccination
5~10ml of kind, more preferably 7~9ml, are most preferably 8ml.
After inoculation, the lentinus edodes strain stick bacterium germination that will obtain of the present invention, takes off bag after mycelial growth purseful, solid stacks to obtain annesl
Mushroom.Mycelium full maturity at this time, have accumulated abundant nutrition, and thalline mycoderma is formed, surrounding bulge, it is preferred that the hair
Bacterium carries out in culturing room.Preferably, the condition during bacterium germination is：20~25 DEG C of temperature, humidity 65~75%, daily ventilation
0.5~1.5 it is small when, inoculation 5~7 days after, every turning in 8~10 days once；More preferably 22~24 DEG C of temperature, humidity 68~
72%, when ventilation 0.8~1.3 is small daily, after being inoculated with 5.5~6.5 days, every turning in 8.5~9.5 days once；It is most preferably warm
23 DEG C, humidity 70% are spent, when ventilation 1 is small daily, after being inoculated with 6 days, every turning in 9 days once.Preferably, the turning be will be upper
Under, bacteria stick inside and outside exchanges mutually.Preferably, contaminated bacteria stick is removed during turning.
In the present invention, bag is taken off after the mycelial growth purseful, it is the annesl stage that solid, which stacks the stage,.Preferably, institute
Condition when stating annesl is：18~22 DEG C of temperature, humidity 55~75%；More preferably：19~21 DEG C of temperature, humidity 60~
70%；Most preferably 20 DEG C of temperature, humidity 65%.Preferably, direct sunlight is avoided during the annesl and reduces time stirred
Number.
After obtaining annesl mushroom, the present invention to obtain annesl mushroom flower bud, grow to obtain radix astragali mushroom.Preferably, it is described
The condition of flower bud is：10~22 DEG C of temperature, 5~10 DEG C of day and night temperature, air humidity 85~95%；More preferably it is：Temperature 12
~20 DEG C, 6~8 DEG C of day and night temperature, air humidity 88~92%.There is white and splits in the mycoderm of flower bud 3~4d bacterium rod surface brown
Line, afterwards long fruiting flower bud.Preferably, the growth conditions is：8~25 DEG C of temperature, air humidity 85~90% divulge information 1 daily
~5h；More preferably：15~20 DEG C of temperature, air humidity 87~89%, divulge information 2~4h daily, is most preferably：18 DEG C of temperature,
Air humidity 88%, divulge information 3h daily.
The present invention provides a kind of cultivation compost of radix astragali mushroom and the cultivation side of radix astragali mushroom including the compost
Method, the present invention, with maize straw, corncob etc. for auxiliary material, make system using radix astragali stalk and stilbene head as the major ingredient of cultivation compost
Standby obtained radix astragali mushroom has reached following effect：
1. reduce cost：Cost, every 1.0 jin of siccatives can be effectively reduced by using discarded radix astragali stalk and stilbene head
Cost can be reduced by more than 0.36 yuan.
2. shorten 8~10d of the Mycelium culture time：The addition of radix astragali stalk promotes the life of mycelia to a certain extent
It is long, be 35~40d using the mushroom liquid bacterial cultivation mycelia purseful time, and the solid spawn inoculation mycelia purseful time be 40~
48d。
3. improve biological efficiency 10%~20%：6~7 liang of produce surviving of son entity of every bag of mushroom is produced using the method for the present invention,
5~6 liang of produce surviving of son entity of every bag of compost based on solid spawn cotton seed hulls.
4. improve the nutritive value of mushroom：Contain the materials such as astragalus polyose, saponin(e, flavones in radix astragali mushroom.
5. mushroom flavor is more preferably, the mushroom cultivated with radix astragali stalk and stilbene head carries the peculiar taste of radix astragali, more fresh
It is tender tasty and refreshing.
Below in conjunction with the embodiment in the present invention, the technical solution in the present invention is clearly and completely described.It is aobvious
So, described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Based on the reality in the present invention
Apply example, those of ordinary skill in the art's all other embodiments obtained without making creative work, all belong to
In the scope of protection of the invention.
Embodiment 1
The mushroom dicaryon mycelium strain block of 5.0 × 5.0 × 2.0mm is inoculated in PDA culture medium and cultivates 6 at 20 DEG C
It obtains mycelium, and mycelium is transferred to lywallzyme：Cellulase：The mass ratio of glusulase is 1.9:1.1:1.1 total concentration is
In 0.9% mixed enzyme solution, at 32 DEG C, 410rpm shaking tables enzymolysis 7h, obtains the body of mycelia quality and mixed enzyme solution described in enzymolysis liquid
Product ratio is 0.8g:5.5ml.
MgSO by obtained enzymolysis liquid using the 0.5mol/L of 2.5 times of enzymolysis liquid volumes4.7H2O solution, which dilutes, to be diluted
Liquid；By dilution G3The filtrate of number sand core funnel filtering；Filtrate is centrifuged into 10min under the conditions of 3000r/min, is moved with sterilizing
Liquid pipe, which pipettes middle level liquid, must purify protoplast suspension；PDA of the obtained purification protoplast suspension in 3.5 times of volumes is cultivated
In liquid at 22 DEG C quiescent culture 46h, obtain Protoplast cuhnre liquid；Obtained Protoplast cuhnre liquid is used into gradient dilution
Method, applies PDA plate and tube culture after 12d is cultivated at 23 DEG C, obtains the monocaryon X of no clamp connection and pair with clamp connection
Nucleome Y.
Two monocaryon X are inoculated on comprehensive PDA tablet at 22 DEG C dark be inverted at a distance of 0.4cm and cultivate 6d, at this time
Two bacterium are intersected, and the mycelium of two bacterium colony laps is taken after two bacterium colonies intersect 5d, goes in comprehensive PDA tablet and cultivates 4d, obtain
To the nucleated mycelium with clamp connection.
Using obtained dicaryon Y or nucleated mycelium as strain.
Obtained strain is aseptically inoculated into fluid nutrient medium by 10% inoculum concentration and is stood at 24 DEG C
50h is cultivated, is sprouted on mycelia liquid medium within, then mushroom liquid bacterial mother is obtained with 135r/min constant-temperature shaking cultures 4.5d
Kind.The fluid nutrient medium includes the component of following mass percentage：Corn flour 1.0%%, analysis for soybean powder 2.0%, yeast extract
0.2%th, dipotassium hydrogen phosphate 0.05%, potassium dihydrogen phosphate 0.10%, magnesium sulfate 0.15% and surplus are water, and the ph of culture medium is certainly
So；The fluid nutrient medium sterilizes under 0.13Mpa 30min and is cooled to less than 30 DEG C.
It is 0.9 that mushroom liquid bacterial parent species, which will be obtained, by the volume ratio of strain and fermentation medium:50 are inoculated into fermentation training
Support in base, the 4.5d that ferments at 24 DEG C of temperature, pressure 0.017Mpa obtains liquid spawn.The fermentation medium includes following quality
The component of percentage composition：Granularity is wheat bran 2%, corn flour 3%, analysis for soybean powder 1%, dipotassium hydrogen phosphate 0.10%, the phosphoric acid of 0.8mm
Potassium dihydrogen 0.15%, magnesium sulfate 0.10%, defoamer 0.2% and surplus are water.
Material bag equipped with compost is sterilized, transfer room when being cooled to less than 25 DEG C after sterilization is interior by sterile working side
Method is inoculated with, and is made a call to three (i.e. three vaccinations) in bacterium bag homonymy, and each vaccination is inoculated with mushroom liquid bacterial 5ml with inoculating gun,
Sealing.The compost includes the component of following parts by weight：Radix astragali stalk and radix astragali stilbene is 30 parts first, maize straw and corncob 30
Part, 17 parts of cotton seed hulls, 20 parts of sawdust, 15 parts of wheat bran, 2 parts of corn flour, 3 parts of analysis for soybean powder, 1 part of gypsum, 0.5 part of lime, phosphoric acid hydrogen
0.10 part of dipotassium, 0.05 part of potassium dihydrogen phosphate, 0.05 part of magnesium sulfate, material-water ratio 1:1.50；Radix astragali stalk and radix astragali stilbene head
Weight ratio is 1:1.1, the weight ratio of maize straw and corncob is 1:0.9.
By the compost after inoculation 20 DEG C of temperature, humidity 75% culturing room in bacterium germination, ventilation 0.8 daily during bacterium germination
Hour, after being inoculated with 7 days, every turning in 8 days once, while remove pollution bacterium bag.Bag is taken off after the long purseful of mycelium, solid stacks,
In 21 DEG C of temperature, 75% time annesl of humidity obtains annesl mushroom, and direct sunlight is avoided during annesl and reduces the number stirred.
After obtaining annesl mushroom, in 10~22 DEG C of temperature, 5~10 DEG C of day and night temperature, flower bud under the conditions of air humidity 85%,
There is white crackle in the mycoderm of flower bud 3d bacterium rod surface brown, afterwards long fruiting flower bud.Mushroom flower bud is in 25 DEG C of temperature, air humidity
Radix astragali mushroom is grown to obtain under conditions of 90%, the 1h that divulges information daily.
Embodiment 2
The mushroom dicaryon mycelium strain block of 5.0 × 5.0 × 2.0mm is inoculated in PDA culture medium and cultivates 4 at 26 DEG C
It obtains mycelium, and mycelium is transferred to lywallzyme：Cellulase：The mass ratio of glusulase is 2.1:1.1:0.9 total concentration is
In 1.1% mixed enzyme solution, at 35 DEG C, 350rpm shaking tables enzymolysis 6h, obtains enzymolysis liquid；The body of the mycelia quality and mixed enzyme solution
Product ratio is 1.2g:5.5ml.
MgSO by obtained enzymolysis liquid using the 0.7mol/L of 1.5 times of enzymolysis liquid volumes4.7H2O solution, which dilutes, to be diluted
Liquid；By dilution G3The filtrate of number sand core funnel filtering；Filtrate is centrifuged into 5min under the conditions of 3500r/min, is moved with sterilizing
Liquid pipe, which pipettes middle level liquid, must purify protoplast suspension；PDA of the obtained purification protoplast suspension in 4.5 times of volumes is cultivated
In liquid at 25 DEG C quiescent culture 50h, obtain Protoplast cuhnre liquid；Obtained Protoplast cuhnre liquid is used into gradient dilution
Method, applies PDA plate and tube culture after 12d is cultivated at 22 DEG C, obtains the monocaryon X of no clamp connection and pair with clamp connection
Nucleome Y.
Two monocaryon X are inoculated on comprehensive PDA tablet at 25 DEG C dark be inverted at a distance of 0.5cm and cultivate 7d, at this time
Two bacterium are intersected, and the mycelium of two bacterium colony laps is taken after two bacterium colonies intersect 7d, goes in comprehensive PDA tablet and cultivates 3d, obtain
To the nucleated mycelium with clamp connection.
Using obtained dicaryon Y or nucleated mycelium as strain.
Obtained strain aseptically is inoculated into fluid nutrient medium to stand at 25 DEG C by 4% inoculum concentration and is trained
45h is supported, is sprouted on mycelia liquid medium within, then mushroom liquid bacterial parent species are obtained with 145r/min constant-temperature shaking cultures 4d.
The fluid nutrient medium includes the component of following mass percentage：Corn flour 2.5%, analysis for soybean powder 1.2%, yeast extract
0.17%th, dipotassium hydrogen phosphate 0.15%, potassium dihydrogen phosphate 0.10%, magnesium sulfate 0.05% and surplus are water, and the pH of culture medium is certainly
So；The fluid nutrient medium sterilizes under 0.14Mpa 30min and is cooled to less than 30 DEG C.
It is 1.1 that mushroom liquid bacterial parent species, which will be obtained, by the volume ratio of strain and fermentation medium:50 are inoculated into fermentation training
Support in base, the 3d that ferments at 27 DEG C of temperature, pressure 0.025Mpa obtains liquid spawn.The fermentation medium includes following quality hundred
Divide the component of content：Granularity is wheat bran 4%, corn flour 1%, analysis for soybean powder 2%, dipotassium hydrogen phosphate 0.15%, the di(2-ethylhexyl)phosphate of 0.4mm
Hydrogen potassium 0.05%, magnesium sulfate 0.07%, defoamer 0.1% and surplus are water.
Material bag equipped with compost is sterilized, transfer room when being cooled to less than 25 DEG C after sterilization is interior by sterile working side
Method is inoculated with, and is made a call to three (i.e. three vaccinations) in bacterium bag homonymy, and each vaccination is inoculated with mushroom liquid bacterial 10ml with inoculating gun,
Sealing.The compost includes the component of following parts by weight：Radix astragali stalk and radix astragali stilbene is 40 parts first, maize straw and corncob 20
Part, 10 parts of cotton seed hulls, 30 parts of sawdust, 12 parts of wheat bran, 3 parts of corn flour, 2 parts of analysis for soybean powder, 0.5 part of gypsum, 1 part of lime, phosphoric acid hydrogen
0.15 part of dipotassium, 0.15 part of potassium dihydrogen phosphate, 0.10 part of magnesium sulfate, material-water ratio 1.25:1.50；Radix astragali stalk and radix astragali stilbene head
Weight ratio be 1:0.9, the weight ratio of maize straw and corncob is 1:1.1.
By the compost after inoculation 25 DEG C of temperature, humidity 68% culturing room in bacterium germination, ventilation 0.5 daily during bacterium germination
Hour, after being inoculated with 5 days, every turning in 10 days once, while remove pollution bacterium bag.Bag is taken off after the long purseful of mycelium, solid stacks,
In 18 DEG C of temperature, 55% time annesl of humidity obtains annesl mushroom, and direct sunlight is avoided during annesl and reduces the number stirred.
After obtaining annesl mushroom, in 12~20 DEG C of temperature, 6~8 DEG C of day and night temperature, flower bud under the conditions of air humidity 95%,
There is white crackle in the mycoderm of flower bud 34d bacterium rod surface brown, afterwards long fruiting flower bud.Mushroom flower bud is in 8 DEG C of temperature, air humidity
Radix astragali mushroom is grown to obtain under conditions of 85%, the 5h that divulges information daily.
Embodiment 3
The mushroom dicaryon mycelium strain block of 5.0 × 5.0 × 2.0mm is inoculated in PDA culture medium to be cultivated 5 days at 28 DEG C
Mycelium is obtained, mycelium is transferred to lywallzyme：Cellulase：The mass ratio of glusulase is 2.1:0.9:0.9 total concentration is
In 1.0% mixed enzyme solution, at 33 DEG C, 450rpm shaking tables enzymolysis 5h, obtains enzymolysis liquid；The body of the mycelia quality and mixed enzyme solution
Product ratio is 1.2g:4.5ml.
MgSO by obtained enzymolysis liquid using the 0.7mol/L of 2 times of enzymolysis liquid volumes4.7H2O solution dilutes to obtain dilution；
By dilution G3The filtrate of number sand core funnel filtering；Filtrate is centrifuged into 8min under the conditions of 3700r/min, with sterilizing pipette
Protoplast suspension must be purified by pipetting middle level liquid；By obtained purification protoplast suspension in the PDA nutrient solutions of 4 times of volumes in
Quiescent culture 48h at 25 DEG C, obtains Protoplast cuhnre liquid；Obtained Protoplast cuhnre liquid is used into gradient dilution method, applies PDA
Tablet cultivates tube culture after 11d at 25 DEG C, obtains the monocaryon X of no clamp connection and the dicaryon Y with clamp connection.
Two monocaryon X are inoculated on comprehensive PDA tablet at 25 DEG C dark be inverted at a distance of 0.6cm and cultivate 5d, at this time
Two bacterium are intersected, and the mycelium of two bacterium colony laps is taken after two bacterium colonies intersect 6d, goes in comprehensive PDA tablet and cultivates 5d, obtain
To the nucleated mycelium with clamp connection.
Using obtained dicaryon Y or nucleated mycelium as strain.
Obtained strain aseptically is inoculated into fluid nutrient medium to stand at 26 DEG C by 2% inoculum concentration and is trained
48h is supported, is sprouted on mycelia liquid medium within, then mushroom liquid bacterial parent species are obtained with 145r/min constant-temperature shaking cultures 4d.
The fluid nutrient medium includes the component of following mass percentage：Corn flour 3.0%, analysis for soybean powder 1.0%, yeast extract 0.1%,
Dipotassium hydrogen phosphate 0.13%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.12% and surplus are water, and the ph of culture medium is natural；It is described
Fluid nutrient medium sterilizes under 0.14Mpa 30min and is cooled to less than 30 DEG C.
It is 1.05 that mushroom liquid bacterial parent species, which will be obtained, by the volume ratio of strain and fermentation medium:50 are inoculated into fermentation training
Support in base, the 5d that ferments at 23 DEG C of temperature, pressure 0.022Mpa obtains liquid spawn.The fermentation medium includes following quality hundred
Divide the component of content：Granularity is wheat bran 5%, corn flour 2.5%, analysis for soybean powder 1.3%, dipotassium hydrogen phosphate 0.05%, the phosphorus of 0.7mm
Acid dihydride potassium 0.12%, magnesium sulfate 0.05% and surplus are water.
Material bag equipped with compost is sterilized, transfer room when being cooled to less than 25 DEG C after sterilization is interior by sterile working side
Method is inoculated with, and is made a call to three (i.e. three vaccinations) in bacterium bag homonymy, and each vaccination is inoculated with mushroom liquid bacterial 7ml with inoculating gun,
Sealing.The compost includes the component of following parts by weight：Radix astragali stalk and radix astragali stilbene is 37 parts first, maize straw and corncob 23
Part, 20 parts of cotton seed hulls, 23 parts of sawdust, 10 parts of wheat bran, 1.5 parts of corn flour, 1.5 parts of analysis for soybean powder, 0.8 part of gypsum, 0.6 part of lime,
0.12 part of dipotassium hydrogen phosphate, 0.08 part of potassium dihydrogen phosphate, 0.0.15 parts of magnesium sulfate, material-water ratio 1:1.35；Radix astragali stalk and Huang
The weight ratio of stilbene stilbene head is 1:0.92, the weight ratio of maize straw and corncob is 1:1.05.
By the compost after inoculation 22 DEG C of temperature, humidity 65% culturing room in bacterium germination, ventilation 1.3 daily during bacterium germination
Hour, after being inoculated with 5 days, every turning in 8.5 days once, while remove pollution bacterium bag.Bag is taken off after the long purseful of mycelium, solid is folded
Put, in 22 DEG C of temperature, 60% time annesl of humidity obtains annesl mushroom, and direct sunlight is avoided during annesl and reduces the number stirred.
After obtaining annesl mushroom, in 12~22 DEG C of temperature, 6~8 DEG C of day and night temperature, flower bud under the conditions of air humidity 92%,
There is white crackle in the mycoderm of flower bud 3d bacterium rod surface brown, afterwards long fruiting flower bud.Mushroom flower bud is in 15 DEG C of temperature, air humidity
Radix astragali mushroom is grown to obtain under conditions of 87%, the 3h that divulges information daily.
Embodiment 4
The mushroom dicaryon mycelium strain block of 5.0 × 5.0 × 2.0mm is inoculated in PDA culture medium and cultivates 5 at 23 DEG C
It obtains mycelium, and mycelium is transferred to lywallzyme：Cellulase：The mass ratio of glusulase is 2:1:1 total concentration is 1.0%
In mixed enzyme solution, at 34 DEG C, 400rpm shaking tables enzymolysis 6h, obtains enzymolysis liquid；The mycelia quality and the volume ratio of mixed enzyme solution are
1.0g:5.0ml。
MgSO by obtained enzymolysis liquid using the 0.6mol/L of 2 times of enzymolysis liquid volumes4.7H2O solution dilutes to obtain dilution；
By dilution G3The filtrate of number sand core funnel filtering；Filtrate is centrifuged into 7min under the conditions of 3500r/min, with sterilizing pipette
Protoplast suspension must be purified by pipetting middle level liquid；By obtained purification protoplast suspension in the PDA nutrient solutions of 4 times of volumes in
Quiescent culture 48h at 23 DEG C, obtains Protoplast cuhnre liquid；Obtained Protoplast cuhnre liquid is used into gradient dilution method, applies PDA
Tablet cultivates tube culture after 10d at 23 DEG C, obtains the monocaryon X of no clamp connection and the dicaryon Y with clamp connection.
Two monocaryon X are inoculated on comprehensive PDA tablet at 23 DEG C dark be inverted at a distance of 0.5cm and cultivate 6d, at this time
Two bacterium are intersected, and the mycelium of two bacterium colony laps is taken after two bacterium colonies intersect 5d, goes in comprehensive PDA tablet and cultivates 3d, obtain
To the nucleated mycelium with clamp connection.
Using obtained dicaryon Y or nucleated mycelium as strain.
Obtained strain aseptically is inoculated into fluid nutrient medium to stand at 25 DEG C by 5% inoculum concentration and is trained
48h is supported, is sprouted on mycelia liquid medium within, then mushroom liquid bacterial parent species are obtained with 140r/min constant-temperature shaking cultures 4d.
The fluid nutrient medium includes the component of following mass percentage：Corn flour 2.0%, analysis for soybean powder 1.5%, yeast extract
0.15%th, dipotassium hydrogen phosphate 0.10%, potassium dihydrogen phosphate 0.10%, magnesium sulfate 0.10% and surplus are water；The Liquid Culture
Base sterilizes under 0.14Mpa 30min and is cooled to less than 30 DEG C.
It is 1. that mushroom liquid bacterial parent species, which will be obtained, by the volume ratio of strain and fermentation medium:50 are inoculated into fermented and cultured
In base, the 4d that ferments at 25 DEG C of temperature, pressure 0.02Mpa obtains liquid spawn.The fermentation medium includes following quality percentage
The component of content：Granularity is wheat bran 3.5%, corn flour 2%, analysis for soybean powder 1.5%, dipotassium hydrogen phosphate 0.1%, the phosphoric acid of 0.6mm
Potassium dihydrogen 0.10%, magnesium sulfate 0.07%, defoamer 0.15% and surplus are water.
Material bag equipped with compost is sterilized, transfer room when being cooled to less than 25 DEG C after sterilization is interior by sterile working side
Method is inoculated with, and is made a call to three (i.e. three vaccinations) in bacterium bag homonymy, and each vaccination is inoculated with mushroom liquid bacterial 8ml with inoculating gun,
Sealing.The compost includes the component of following parts by weight：Radix astragali stalk and radix astragali stilbene is 35 parts first, maize straw and corncob 25
Part, 15 parts of cotton seed hulls, 25 parts of sawdust, 13 parts of wheat bran, 2.5 parts of corn flour, 2.5 parts of analysis for soybean powder, 0.7 part of gypsum, 0.7 part of lime,
0.10 part of dipotassium hydrogen phosphate, 0.10 part of potassium dihydrogen phosphate, 0.10 part of magnesium sulfate, material-water ratio 1:1.4；Radix astragali stalk and radix astragali stilbene
The weight ratio of head is 1:1, the weight ratio of maize straw and corncob is 1:1.
By the compost after inoculation 23 DEG C of temperature, humidity 70% culturing room in bacterium germination, ventilation 1 is small daily during bacterium germination
When, after being inoculated with 6 days, every turning in 9 days once, while remove pollution bacterium bag.Bag is taken off after the long purseful of mycelium, solid stacks,
20 DEG C of temperature, 65% time annesl of humidity obtain annesl mushroom, and direct sunlight is avoided during annesl and reduces the number stirred.
After obtaining annesl mushroom, in 12~20 DEG C of temperature, 6~8 DEG C of day and night temperature, flower bud under the conditions of air humidity 90%,
There is white crackle in the mycoderm of flower bud 3d bacterium rod surface brown, afterwards long fruiting flower bud.Mushroom flower bud is in 18 DEG C of temperature, air humidity
Radix astragali mushroom is grown to obtain under conditions of 88%, the 3h that divulges information daily.
Embodiment 5
With common commercially available cultivation compost cultivation, other conditions are identical with the embodiment of the present application 4, as a control group, with this
The radix astragali mushroom that application embodiment 1-4 is prepared is compared, and concrete outcome is as shown in table 1.
The radix astragali cultivating champignon compost that the application provides can be effective by using discarded radix astragali stalk and stilbene head
Cost is reduced, compared with control group, every 1.0 jin of siccatives can reduce cost more than 0.36 yuan.
The addition of radix astragali stalk promotes the growth of mycelia to a certain extent, is expired using mushroom liquid bacterial cultivation mycelia
The bag time is 35~40d, and the control group solid spawn inoculation mycelia purseful time is 40~48d.
6~7 liang of produce surviving of son entity of every bag of mushroom is produced using the method for the present invention, based on the solid spawn cotton seed hulls of control group
5~6 liang of produce surviving of son entity of every bag of compost, compared with control group, biological efficiency improves 10%~20%.
The mushroom cultivated with radix astragali stalk and stilbene head carries the peculiar taste of radix astragali, more fresh and tender tasty and refreshing, radix astragali mushroom
In containing the material such as astragalus polyose, saponin(e, flavones, improve the nutritive value of mushroom.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of cultivation compost of radix astragali mushroom, includes the component of following parts by weight：
Radix astragali stalk and radix astragali stilbene is 30~40 parts first, maize straw and 20~30 parts of corncob, 10~20 parts of cotton seed hulls, sawdust 20
~30 parts, 10~15 parts of wheat bran, 2~3 parts of corn flour, 2~3 parts of analysis for soybean powder, 0.5~1 part of gypsum, 0.5~1 part of lime, phosphoric acid
0.05~0.15 part of hydrogen dipotassium, 0.05~0.15 part of potassium dihydrogen phosphate, 0.05~0.15 part of magnesium sulfate, material-water ratio 1:1.25~
1.50；
The weight ratio of radix astragali stalk and radix astragali the stilbene head is 1:0.9~1.1；The weight ratio of the maize straw and corncob is
1:0.9~1.1.
2. a kind of radix astragali mushroom cultivating method, comprises the following steps：
1) it will be inoculated with when sterilizing equipped with the material bag of compost described in claim 1, be cooled to less than 25 DEG C, obtain lentinus edodes strain stick；
2) the lentinus edodes strain stick bacterium germination for obtaining the step 1), bag is taken off after mycelial growth purseful, and solid stacks to obtain annesl mushroom
Bacteria stick；
3) by annesl lentinus edodes strain stick flower bud that the step 2) obtains, grow to obtain radix astragali mushroom.
3. cultural method according to claim 2, it is characterised in that the preparation side of strain used in inoculation in the step 1)
Method comprises the following steps：
A. the dicaryon mycelium of mushroom is inoculated in PDA culture medium and cultivates to obtain mycelium, the mycelium is transferred to mixed enzyme
Liquid digests, and obtains enzymolysis liquid；The mixed enzymolysis liquid includes lywallzyme, cellulase and glusulase；
B. the obtained enzymolysis liquids of the step a are diluted, filtered and centrifuged successively, take middle level liquid to purify protoplast suspension；
C. the purification protoplast suspension step b obtained is cultivated in PDA nutrient solutions, obtains Protoplast cuhnre liquid；
D. the Protoplast cuhnre liquid step c obtained uses gradient dilution method, applies tube after 8~12d of PDA plate culture
Culture, the mycelium that picking single bacterium colony is sprouted, carries out microexamination, obtains the monocaryon X of no clamp connection and with lock shape
United dicaryon Y, distinguishes tube culture by X, Y；
E. the obtained monocaryon X of the step d are only mated, that is, takes 2 monocaryon X, be inoculated with a distance of 0.4~0.6cm
In being cultivated in comprehensive PDA culture medium, the mycelium of two bacterium colony laps is taken after two bacterium colonies intersect 5~7d, goes to comprehensive PDA
More than 3d is cultivated in culture medium, obtains the nucleated mycelium with clamp connection, expands culture；
F. the nucleated mycelium step d obtained dicaryon Y or step e obtained is as strain.
4. cultural method according to claim 3, it is characterised in that by mass percentage, mixed enzyme in the step a
The total concentration of liquid is 0.9~1.1%.
5. cultural method according to claim 3, it is characterised in that lywallzyme in mixed enzyme solution in the step a：Fiber
Plain enzyme：The weight ratio of glusulase is 1.9~2.1:0.9~1.1:0.9~1.1.
6. according to the cultural method described in claim 3~5 any one, it is characterised in that the step f is gone back after obtaining strain
Including：The strain liquid culture that will be obtained in step f, obtains liquid spawn.
7. cultural method according to claim 6, it is characterised in that the preparation method of the liquid spawn includes following step
Suddenly：
(1) strain that the inoculation step f is obtained is inoculated into fluid nutrient medium and carries out liquid fermentation culture, obtains mushroom liquid bacteria
Kind parent species；
(2) the mushroom liquid bacterial parent species that the step (1) obtains are connected in fermentation medium, carry out liquid fermentation and cultivate
Liquid spawn.
8. cultural method according to claim 7, it is characterised in that the culture of liquid fermentation and culture in the step (1)
Base includes the component of following mass percentage：
Corn flour 1.0%~3.0%, analysis for soybean powder 1.0%~2.0%, yeast extract 0.1%~0.2%, dipotassium hydrogen phosphate 0.05%
~0.15%, potassium dihydrogen phosphate 0.05%~0.15%, magnesium sulfate 0.05%~0.15% and surplus are water；
Fermentation medium includes the component of following mass percentage in the step (2)：
Wheat bran 2%~5%, corn flour 1%~3%, analysis for soybean powder 1~2%, dipotassium hydrogen phosphate 0.05%~0.15%, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.05%~0.15%, magnesium sulfate 0.05%~0.10% and surplus are water.
9. cultural method according to claim 2, it is characterised in that the condition in the step 2) during bacterium germination is：Temperature
20~25 DEG C, humidity 65~75%, when ventilation 0.5~1.5 is small daily, after being inoculated with 5~7 days, every turning in 8~10 days once.
10. cultural method according to claim 2, it is characterised in that the condition of flower bud is in the step 3)：Temperature 10
~22 DEG C, 5~10 DEG C of day and night temperature, air humidity 85~95%；
The condition of growth is in the step 3)：8~25 DEG C of temperature, air humidity 85~90%, when ventilation 1~5 is small daily.
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| 李小轩: "玉米秸秆黄芪茎叶栽培姬菇技术", 《食用菌》 * |
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Application publication date: 20180420 |