CN106635949B - 细胞培养基和方法 - Google Patents

细胞培养基和方法 Download PDF

Info

Publication number
CN106635949B
CN106635949B CN201611042079.XA CN201611042079A CN106635949B CN 106635949 B CN106635949 B CN 106635949B CN 201611042079 A CN201611042079 A CN 201611042079A CN 106635949 B CN106635949 B CN 106635949B
Authority
CN
China
Prior art keywords
cells
medium
beads
feed
microsuspension
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201611042079.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN106635949A (zh
Inventor
R·菲克
S·巴雷特
江舟
D·贾德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of CN106635949A publication Critical patent/CN106635949A/zh
Application granted granted Critical
Publication of CN106635949B publication Critical patent/CN106635949B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02—Making microcapsules or microballoons
    • B01J13/06—Making microcapsules or microballoons by phase separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02—Making microcapsules or microballoons
    • B01J13/20—After-treatment of capsule walls, e.g. hardening
    • B01J13/22—Coating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C67/00—Shaping techniques not covered by groups B29C39/00 - B29C65/00, B29C70/00 or B29C73/00
    • B29C67/24—Shaping techniques not covered by groups B29C39/00 - B29C65/00, B29C70/00 or B29C73/00 characterised by the choice of material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29L—INDEXING SCHEME ASSOCIATED WITH SUBCLASS B29C, RELATING TO PARTICULAR ARTICLES
    • B29L2009/00—Layered products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/32—Polylysine, polyornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/72—Chitin, chitosan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/76—Agarose, agar-agar
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/78—Cellulose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/80—Hyaluronan

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201611042079.XA 2011-12-22 2012-12-21 细胞培养基和方法 Active CN106635949B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161579432P 2011-12-22 2011-12-22
US61/579,432 2011-12-22
CN201280068225.XA CN104080905B (zh) 2011-12-22 2012-12-21 细胞培养基和方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201280068225.XA Division CN104080905B (zh) 2011-12-22 2012-12-21 细胞培养基和方法

Publications (2)

Publication Number Publication Date
CN106635949A CN106635949A (zh) 2017-05-10
CN106635949B true CN106635949B (zh) 2020-05-19

Family

ID=47557531

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201611042079.XA Active CN106635949B (zh) 2011-12-22 2012-12-21 细胞培养基和方法
CN201280068225.XA Active CN104080905B (zh) 2011-12-22 2012-12-21 细胞培养基和方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201280068225.XA Active CN104080905B (zh) 2011-12-22 2012-12-21 细胞培养基和方法

Country Status (6)

Country Link
US (4) US9410118B2 (enExample)
EP (2) EP2794853B1 (enExample)
JP (4) JP6216327B2 (enExample)
KR (1) KR102039494B1 (enExample)
CN (2) CN106635949B (enExample)
WO (1) WO2013096858A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10669522B2 (en) 2011-02-07 2020-06-02 Life Technologies Corporation Compositions and methods for stabilizing susceptible compounds
WO2013096858A1 (en) * 2011-12-22 2013-06-27 Life Technologies Corporation Cell culture media and methods
WO2015003773A1 (en) * 2013-07-11 2015-01-15 Merck Patent Gmbh Cell culture media
US10487308B2 (en) * 2014-01-23 2019-11-26 Nissan Chemical Corporation Culture medium composition
US20150275167A1 (en) * 2014-03-28 2015-10-01 Corning Incorporated Composition and method for cell culture sustained release
AU2015243961A1 (en) * 2014-04-10 2016-10-27 Bayer Healthcare Llc Compounded media powder formulation and method of preparation of liquid medium for cell culture
PL3303562T3 (pl) * 2015-06-08 2023-05-08 Corning Incorporated Formulacja susząca dla mikronośników hydrożelowych
JP7166169B2 (ja) * 2015-11-20 2022-11-07 キアゲン ゲーエムベーハー 細胞外核酸の滅菌のための滅菌組成物を調製する方法
US10822584B2 (en) * 2015-12-17 2020-11-03 Life Technologies Corporation Pellets used in cell culture and methods of making thereof
US20190292515A1 (en) * 2015-12-30 2019-09-26 Life Technologies Corporation Layered cell culture media particles and methods of making thereof
CN106222236A (zh) * 2016-07-27 2016-12-14 郑州点石生物技术有限公司 血液中微生物检测试剂及其制备方法
CN109395095B (zh) * 2017-09-18 2021-09-24 武汉原生原代生物医药科技有限公司 体内用生物膜及其制备方法和用途
KR102853452B1 (ko) * 2018-07-27 2025-09-03 아지노모토 가부시키가이샤 동물 세포의 부유 배양용 첨가물, 부유 배양용 배지 및 부유 배양 방법
CN111849879A (zh) * 2020-08-07 2020-10-30 张娇 一种细胞培养基及细胞培养方法
CN112553116A (zh) * 2020-12-23 2021-03-26 邢箫 一种益生菌培养基及益生菌制剂
AU2023374004A1 (en) * 2022-10-31 2025-05-01 Samsung Bioepis Co., Ltd. Method for preparing high-concentration liquid medium

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3870061D1 (de) * 1987-06-19 1992-05-21 Univ Temple Mikrokapseln mit gelenkter freigabe und verfahren zu deren herstellung.
JPS6413986A (en) * 1987-07-08 1989-01-18 Mitsuiwa Eng Kk Microcapsule for cultivating cell and production thereof
JP2944419B2 (ja) * 1993-05-10 1999-09-06 ノバルティス アクチエンゲゼルシャフト 持続性遊離組成物中の薬理学的活性成分の安定
US6281015B1 (en) * 1994-12-16 2001-08-28 Children's Medical Center Corp. Localized delivery of factors enhancing survival of transplanted cells
EP0953041A4 (en) * 1996-08-30 2003-01-29 Life Technologies Inc SERUM-FREE CULTURAL MEDIUM FOR MAMMAL CELLS AND THEIR USE
US6383810B2 (en) * 1997-02-14 2002-05-07 Invitrogen Corporation Dry powder cells and cell culture reagents and methods of production thereof
ES2169980B1 (es) * 1999-12-17 2003-11-01 Lipotec Sa Microcapsulas para la liberacion prolongada de farmacos.
JP2002114667A (ja) * 2000-10-11 2002-04-16 Ltt Institute Co Ltd 徐放性製剤用組成物、その製造法および徐放性マイクロカプセル製剤
AU2002232406C1 (en) * 2000-11-06 2009-03-05 Invitrogen Corporation Dry powder cells and cell culture reagents and methods of production thereof
ATE243028T1 (de) * 2001-07-11 2003-07-15 Applied Pharma Res Fettlösliche substanzen enthaltende granulate und verfahren zu ihrer herstellung
DE60235835D1 (de) * 2001-11-30 2010-05-12 Life Technologies Corp Zellkulturmedien
US6974592B2 (en) * 2002-04-11 2005-12-13 Ocean Nutrition Canada Limited Encapsulated agglomeration of microcapsules and method for the preparation thereof
CA2552690C (en) 2002-12-31 2014-12-09 Ultra-Sonic Technologies, L.L.C. Transdermal delivery using encapsulated agent activated by ultrasound and/or heat
ES2389441T3 (es) 2004-01-08 2012-10-26 North Carolina State University Métodos y dispositivos para el microencapsulamiento de células
US7740861B2 (en) 2004-06-16 2010-06-22 University Of Massachusetts Drug delivery product and methods
WO2007094000A2 (en) 2006-02-15 2007-08-23 Botanocap Ltd. Applications of microencapsulated essential oils
BR112012009644A2 (pt) * 2009-10-21 2015-09-29 Dow Pharmaceutical Sciences método para umedecer um pó contendo o peróxido de benzoíla
US20110262965A1 (en) 2010-04-23 2011-10-27 Life Technologies Corporation Cell culture medium comprising small peptides
WO2011136274A1 (ja) * 2010-04-28 2011-11-03 株式会社フジクラ 光ファイバケーブル
CN102971296B (zh) * 2010-04-30 2015-11-25 百时美施贵宝公司 包含n-(4-(2-氨基-3-氯吡啶-4-基氧基)-3-氟苯基)-4-乙氧基-1-(4-氟苯基)-2-氧代-1，2-二氢吡啶-3-羧酰胺的药物组合物
US10669522B2 (en) * 2011-02-07 2020-06-02 Life Technologies Corporation Compositions and methods for stabilizing susceptible compounds
WO2013096858A1 (en) * 2011-12-22 2013-06-27 Life Technologies Corporation Cell culture media and methods

Also Published As

Publication number Publication date
JP2015503329A (ja) 2015-02-02
JP2021087446A (ja) 2021-06-10
JP6457041B2 (ja) 2019-01-23
KR102039494B1 (ko) 2019-11-01
EP3702442A1 (en) 2020-09-02
CN104080905A (zh) 2014-10-01
US12203095B2 (en) 2025-01-21
WO2013096858A1 (en) 2013-06-27
US20160376548A1 (en) 2016-12-29
JP2019062901A (ja) 2019-04-25
CN104080905B (zh) 2016-11-16
EP2794853A1 (en) 2014-10-29
JP6216327B2 (ja) 2017-10-18
US9410118B2 (en) 2016-08-09
CN106635949A (zh) 2017-05-10
EP3702442B1 (en) 2025-06-25
JP6843824B2 (ja) 2021-03-17
US20150064785A1 (en) 2015-03-05
JP2018038404A (ja) 2018-03-15
US10066200B2 (en) 2018-09-04
JP7217765B2 (ja) 2023-02-03
US20200332250A1 (en) 2020-10-22
EP2794853B1 (en) 2020-04-22
US20180346872A1 (en) 2018-12-06
KR20140113693A (ko) 2014-09-24

Similar Documents

Publication Publication Date Title
CN106635949B (zh) 细胞培养基和方法
EP2089511B1 (en) In vitro expansion of postpartum-derived cells using microcarriers
US11028361B2 (en) Compositions and methods for stabilizing susceptible compounds
SE445116B (sv) Sett att odla celler pa mikroberare med fibronektinytskikt
JP4233646B2 (ja) 種々の基質の安定性および／または貯蔵寿命を増加させる方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant