JP6216327B2 - 細胞培地および方法 - Google Patents

細胞培地および方法 Download PDF

Info

Publication number
JP6216327B2
JP6216327B2 JP2014548973A JP2014548973A JP6216327B2 JP 6216327 B2 JP6216327 B2 JP 6216327B2 JP 2014548973 A JP2014548973 A JP 2014548973A JP 2014548973 A JP2014548973 A JP 2014548973A JP 6216327 B2 JP6216327 B2 JP 6216327B2
Authority
JP
Japan
Prior art keywords
medium
feed
cell culture
cells
supplement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2014548973A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015503329A (ja
JP2015503329A5 (enExample
Inventor
リチャード ファイク
リチャード ファイク
ショーン バレット
ショーン バレット
チョウ チャン
チョウ チャン
デビッド ジャド
デビッド ジャド
Original Assignee
ライフ テクノロジーズ コーポレーション
ライフ テクノロジーズ コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ライフ テクノロジーズ コーポレーション, ライフ テクノロジーズ コーポレーション filed Critical ライフ テクノロジーズ コーポレーション
Publication of JP2015503329A publication Critical patent/JP2015503329A/ja
Publication of JP2015503329A5 publication Critical patent/JP2015503329A5/ja
Application granted granted Critical
Publication of JP6216327B2 publication Critical patent/JP6216327B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02—Making microcapsules or microballoons
    • B01J13/06—Making microcapsules or microballoons by phase separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02—Making microcapsules or microballoons
    • B01J13/20—After-treatment of capsule walls, e.g. hardening
    • B01J13/22—Coating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C67/00—Shaping techniques not covered by groups B29C39/00 - B29C65/00, B29C70/00 or B29C73/00
    • B29C67/24—Shaping techniques not covered by groups B29C39/00 - B29C65/00, B29C70/00 or B29C73/00 characterised by the choice of material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29L—INDEXING SCHEME ASSOCIATED WITH SUBCLASS B29C, RELATING TO PARTICULAR ARTICLES
    • B29L2009/00—Layered products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/32—Polylysine, polyornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/72—Chitin, chitosan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/76—Agarose, agar-agar
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/78—Cellulose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/80—Hyaluronan

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mechanical Engineering (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
JP2014548973A 2011-12-22 2012-12-21 細胞培地および方法 Active JP6216327B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161579432P 2011-12-22 2011-12-22
US61/579,432 2011-12-22
PCT/US2012/071411 WO2013096858A1 (en) 2011-12-22 2012-12-21 Cell culture media and methods

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2017181983A Division JP6457041B2 (ja) 2011-12-22 2017-09-22 細胞培地および方法

Publications (3)

Publication Number Publication Date
JP2015503329A JP2015503329A (ja) 2015-02-02
JP2015503329A5 JP2015503329A5 (enExample) 2016-01-28
JP6216327B2 true JP6216327B2 (ja) 2017-10-18

Family

ID=47557531

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2014548973A Active JP6216327B2 (ja) 2011-12-22 2012-12-21 細胞培地および方法
JP2017181983A Active JP6457041B2 (ja) 2011-12-22 2017-09-22 細胞培地および方法
JP2018236870A Active JP6843824B2 (ja) 2011-12-22 2018-12-19 細胞培地および方法
JP2021026972A Active JP7217765B2 (ja) 2011-12-22 2021-02-24 細胞培地および方法

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2017181983A Active JP6457041B2 (ja) 2011-12-22 2017-09-22 細胞培地および方法
JP2018236870A Active JP6843824B2 (ja) 2011-12-22 2018-12-19 細胞培地および方法
JP2021026972A Active JP7217765B2 (ja) 2011-12-22 2021-02-24 細胞培地および方法

Country Status (6)

Country Link
US (4) US9410118B2 (enExample)
EP (2) EP3702442B1 (enExample)
JP (4) JP6216327B2 (enExample)
KR (1) KR102039494B1 (enExample)
CN (2) CN106635949B (enExample)
WO (1) WO2013096858A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107699540A (zh) 2011-02-07 2018-02-16 生命技术公司 用于稳定敏感化合物的组合物和方法
JP6216327B2 (ja) * 2011-12-22 2017-10-18 ライフ テクノロジーズ コーポレーション 細胞培地および方法
EP3019594B1 (en) * 2013-07-11 2020-12-02 Merck Patent GmbH Cell culture media
KR102359148B1 (ko) * 2014-01-23 2022-02-08 닛산 가가쿠 가부시키가이샤 배양 배지 조성물
US20150275167A1 (en) * 2014-03-28 2015-10-01 Corning Incorporated Composition and method for cell culture sustained release
CN106459907B (zh) * 2014-04-10 2020-11-06 拜尔健康护理有限责任公司 复合培养基粉末制剂和制备用于细胞培养的液体培养基的方法
CN107750274A (zh) * 2015-06-08 2018-03-02 康宁股份有限公司 用于水凝胶微载体的干燥制剂
CA3005694A1 (en) * 2015-11-20 2017-05-26 Qiagen Gmbh Method of preparing sterilized compositions for stabilization of extracellular nucleic acids
KR20180091923A (ko) * 2015-12-17 2018-08-16 라이프 테크놀로지스 코포레이션 세포 배양에 이용되는 펠릿 및 이의 제조 방법
RU2762292C2 (ru) * 2015-12-30 2021-12-17 Лайф Текнолоджиз Корпорейшн Слоистые частицы клеточной культуры и способы их получения
CN106222236A (zh) * 2016-07-27 2016-12-14 郑州点石生物技术有限公司 血液中微生物检测试剂及其制备方法
CN109395095B (zh) * 2017-09-18 2021-09-24 武汉原生原代生物医药科技有限公司 体内用生物膜及其制备方法和用途
EP3831932A4 (en) * 2018-07-27 2021-09-15 Ajinomoto Co., Inc. SUSPENSION CULTURE ADDITIVE, SUSPENSION CULTURE MEDIA AND SUSPENSION CULTURE METHOD FOR ANIMAL CELLS
CN111849879A (zh) * 2020-08-07 2020-10-30 张娇 一种细胞培养基及细胞培养方法
CN112553116A (zh) * 2020-12-23 2021-03-26 邢箫 一种益生菌培养基及益生菌制剂
KR20250099169A (ko) * 2022-10-31 2025-07-01 삼성바이오에피스 주식회사 고농도 액체 배지 제조 방법

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3870061D1 (de) * 1987-06-19 1992-05-21 Univ Temple Mikrokapseln mit gelenkter freigabe und verfahren zu deren herstellung.
JPS6413986A (en) * 1987-07-08 1989-01-18 Mitsuiwa Eng Kk Microcapsule for cultivating cell and production thereof
EP0626170A3 (en) * 1993-05-10 1996-03-27 Sandoz Ltd Stabilization of pharmacologically active compounds in delayed release agents.
US6281015B1 (en) * 1994-12-16 2001-08-28 Children's Medical Center Corp. Localized delivery of factors enhancing survival of transplanted cells
EP2243827B2 (en) * 1996-08-30 2017-11-22 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
US6383810B2 (en) 1997-02-14 2002-05-07 Invitrogen Corporation Dry powder cells and cell culture reagents and methods of production thereof
ES2169980B1 (es) * 1999-12-17 2003-11-01 Lipotec Sa Microcapsulas para la liberacion prolongada de farmacos.
JP2002114667A (ja) * 2000-10-11 2002-04-16 Ltt Institute Co Ltd 徐放性製剤用組成物、その製造法および徐放性マイクロカプセル製剤
AU2002232406C1 (en) * 2000-11-06 2009-03-05 Invitrogen Corporation Dry powder cells and cell culture reagents and methods of production thereof
EP1275377B1 (en) 2001-07-11 2003-06-18 Apr Applied Pharma Research S.A. Granulates containing liposoluble substances and process of preparation thereof
WO2003048313A2 (en) * 2001-11-30 2003-06-12 Invitrogen Corporation Cell culture media
US6974592B2 (en) * 2002-04-11 2005-12-13 Ocean Nutrition Canada Limited Encapsulated agglomeration of microcapsules and method for the preparation thereof
US20060034904A1 (en) 2002-12-31 2006-02-16 Ultra-Sonic Technologies, L.L.C. Transdermal delivery using emcapsulated agent activated by ultrasound and or heat
WO2005071060A2 (en) 2004-01-08 2005-08-04 North Carolina State University Methods and devices for microencapsulation of cells
US7740861B2 (en) 2004-06-16 2010-06-22 University Of Massachusetts Drug delivery product and methods
WO2007094000A2 (en) 2006-02-15 2007-08-23 Botanocap Ltd. Applications of microencapsulated essential oils
EP2490528A4 (en) * 2009-10-21 2013-06-12 Dow Pharmaceutical Sciences METHOD FOR ANCHORING A POWDER CONTAINING BENZOYL PEROXIDE
US20110262965A1 (en) 2010-04-23 2011-10-27 Life Technologies Corporation Cell culture medium comprising small peptides
JP5802047B2 (ja) * 2010-04-28 2015-10-28 株式会社フジクラ 光ファイバケーブル
WO2011137274A1 (en) 2010-04-30 2011-11-03 Bristol-Myers Squibb Company Pharmaceutical compositions comprising n-(4-(2-amino-3-chloropyridin-4-yloxy)-3-fluorophenyl)-4-ethoxy-l-(4-fluorophenyl)-2-oxo- 1,2-dihydropyridine-3 -carboxamide
CN107699540A (zh) * 2011-02-07 2018-02-16 生命技术公司 用于稳定敏感化合物的组合物和方法
JP6216327B2 (ja) * 2011-12-22 2017-10-18 ライフ テクノロジーズ コーポレーション 細胞培地および方法

Also Published As

Publication number Publication date
JP2019062901A (ja) 2019-04-25
CN104080905A (zh) 2014-10-01
CN104080905B (zh) 2016-11-16
EP3702442B1 (en) 2025-06-25
JP2021087446A (ja) 2021-06-10
US20200332250A1 (en) 2020-10-22
US20150064785A1 (en) 2015-03-05
US9410118B2 (en) 2016-08-09
WO2013096858A1 (en) 2013-06-27
EP2794853A1 (en) 2014-10-29
US20160376548A1 (en) 2016-12-29
JP6843824B2 (ja) 2021-03-17
JP7217765B2 (ja) 2023-02-03
JP2015503329A (ja) 2015-02-02
US20180346872A1 (en) 2018-12-06
EP2794853B1 (en) 2020-04-22
CN106635949B (zh) 2020-05-19
KR102039494B1 (ko) 2019-11-01
CN106635949A (zh) 2017-05-10
US10066200B2 (en) 2018-09-04
JP6457041B2 (ja) 2019-01-23
EP3702442A1 (en) 2020-09-02
KR20140113693A (ko) 2014-09-24
JP2018038404A (ja) 2018-03-15
US12203095B2 (en) 2025-01-21

Similar Documents

Publication Publication Date Title
JP6457041B2 (ja) 細胞培地および方法
US11028361B2 (en) Compositions and methods for stabilizing susceptible compounds
JP4233646B2 (ja) 種々の基質の安定性および／または貯蔵寿命を増加させる方法
GB2386905A (en) Carrier for cell culture and method for culturing cells
US20220380723A1 (en) Methods to produce defined, spherical, bio-degradable macroporous microcarrier/hydrogels for cellular agriculture
Ceaglio et al. 6.2 High Cell Density Cultivation Process

Legal Events

Date Code Title Description
RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20150312

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151201

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20151201

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20161021

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20161026

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170124

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170310

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20170828

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20170922

R150 Certificate of patent or registration of utility model

Ref document number: 6216327

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250