CN106631450B - Plant fertilizer and preparation method thereof - Google Patents
Plant fertilizer and preparation method thereof Download PDFInfo
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- CN106631450B CN106631450B CN201611185843.9A CN201611185843A CN106631450B CN 106631450 B CN106631450 B CN 106631450B CN 201611185843 A CN201611185843 A CN 201611185843A CN 106631450 B CN106631450 B CN 106631450B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a plant fertilizer which is prepared from the following raw materials in parts by weight: 40-60 parts of aspergillus oryzae culture, 20-30 parts of shell, 40-60 parts of lettuce leaf, 10-20 parts of hawthorn, 5-10 parts of bone meal, 5-10 parts of honey, 0.5-1 part of yeast culture and 0.05-0.1 part of growth regulator; the Aspergillus oryzae culture is prepared by inoculating Aspergillus oryzae into mixture of cooked semen glycines and wheat flour, and fermenting for 2-3 days; the yeast culture is obtained by inoculating yeast into liquid wort culture medium, and culturing for 2-3 days. The plant fertilizer provided by the invention takes the extract of natural materials as the raw material, contains rich nutrient substances, can promote the growth of plants, avoids environmental pollution, is particularly suitable for newly transplanted plants, and helps the newly transplanted plants to adapt to the new environment quickly. In addition, the invention also provides a preparation method of the plant fertilizer.
Description
Technical Field
The invention belongs to the technical field of agricultural fertilizers, and particularly relates to a plant fertilizer and a manufacturing method thereof.
Background
With the increasing demand of people for indoor green plant decoration, various plants cultivated in soil or cultivated in soilless culture gradually appear. Due to the limitation of the planting area of indoor plants, before the plants are trimmed into bonsais suitable for indoor placement, large-area centralized cultivation is generally performed in a greenhouse. The application of plant fertilizers is particularly important in order to enable plants to grow rapidly.
The existing plant fertilizer generally adopts urea, potash fertilizer and other chemical fertilizers, and long-time application of the chemical fertilizers can cause soil hardening, accelerate soil acidification and increase of toxic substances in the soil, and lead to soil impoverishment in severe cases, environmental pollution and inconvenience for long-term use. Therefore, it is necessary to develop a plant fertilizer using an extract of a natural material as a raw material so as to promote the growth of plants while avoiding environmental pollution.
Disclosure of Invention
According to the plant fertilizer and the preparation method thereof provided by the invention, the extract of natural materials is used as a raw material, so that the plant growth can be promoted, and the environmental pollution can be avoided.
The invention provides a plant fertilizer which is prepared from the following raw materials in parts by weight: 40-60 parts of aspergillus oryzae culture, 20-30 parts of shell, 40-60 parts of lettuce leaf, 10-20 parts of hawthorn, 5-10 parts of bone meal, 5-10 parts of honey, 0.5-1 part of yeast culture and 0.05-0.1 part of growth regulator;
the Aspergillus oryzae culture is prepared by inoculating Aspergillus oryzae into mixture of cooked semen glycines and wheat flour, and fermenting for 2-3 days;
the yeast culture is obtained by inoculating yeast into liquid wort culture medium, and culturing for 2-3 days.
Preferably, in the plant fertilizer, the aspergillus oryzae is aspergillus oryzae Hu brewing 3.042 or aspergillus oryzae Hu brewing 1.08.
Preferably, in the plant fertilizer, the yeast is saccharomyces cerevisiae.
Preferably, in the plant fertilizer, the growth regulator is one or more of gibberellin, ethephon, naphthylacetic acid and 0.6g/100ml sodium chloride solution.
Preferably, the plant fertilizer is prepared from the following raw materials in parts by weight: 50 parts of aspergillus oryzae culture, 25 parts of shell, 50 parts of lettuce leaf, 15 parts of hawthorn, 10 parts of bone meal, 10 parts of honey, 0.5 part of saccharomycete culture and 0.05 part of growth regulator.
The invention also provides a preparation method of the plant fertilizer, which comprises the following steps:
step 1, preparation of Aspergillus oryzae culture
Step 1.1, activating aspergillus oryzae strains, and eluting aspergillus oryzae spores by using distilled water to obtain an aspergillus oryzae spore suspension;
step 1.2, soaking soybeans in water for 4-6h, draining, steaming, and cooling to 30-40 ℃ to obtain cooked soybeans; grinding wheat into powder, and sieving with 40 mesh sieve to obtain wheat flour; uniformly mixing the cooked soybean and the wheat flour according to the mass ratio of 1:1 to obtain an aspergillus oryzae culture medium;
step 1.3, inoculating an aspergillus oryzae spore suspension into an aspergillus oryzae culture medium, wherein 5-10ml of the aspergillus oryzae spore suspension is inoculated into each kilogram of aspergillus oryzae culture medium, uniformly mixing, and then culturing at 28-32 ℃ for 2-3 days to obtain an aspergillus oryzae culture;
step 2, preparation of Yeast culture
Inoculating yeast into liquid malt extract culture medium, and culturing at 28-30 deg.C for 2-3d to obtain yeast culture;
step 3, weighing the following components in parts by weight: 40-60 parts of aspergillus oryzae culture, 20-30 parts of shell, 40-60 parts of lettuce leaf, 10-20 parts of hawthorn, 5-10 parts of bone meal, 5-10 parts of honey, 0.5-1 part of yeast culture and 0.05-0.1 part of growth regulator;
step 4, respectively crushing the shells and the hawthorns weighed in the step 3 to obtain shell powder and hawthorn powder; the lettuce She Jiancheng fragments weighed in the step 3 are crushed to obtain lettuce leaf fragments;
uniformly mixing shell powder, hawthorn powder, lettuce leaf fragments, the bone meal weighed in the step 3, honey, an aspergillus oryzae culture and a yeast culture, and fermenting for 2d at the temperature of 30 ℃ to obtain a composite fermentation product;
step 5, adding distilled water into the composite fermentation product, continuously stirring for 30min at 50 ℃, filtering, respectively collecting filtrate and filter residue, and evaporating the obtained filtrate to dryness to obtain a water extract;
adding a mixture of dichloromethane and diethyl ether into the obtained filter residue, wherein the volume ratio of dichloromethane to diethyl ether is 1;
step 6, adding 1mol/L disodium hydrogen phosphate solution into the water extract, wherein the ratio of the aspergillus oryzae culture to the disodium hydrogen phosphate solution is 100g;
adding 1mol/L sodium dihydrogen phosphate solution into the organic solvent extract, wherein the volume of the sodium dihydrogen phosphate solution is the same as that of the disodium hydrogen phosphate solution, and obtaining organic fertilizer solution;
and (3) uniformly mixing the water-soluble fertilizer solution and the organic fertilizer solution, and finally adding the growth regulator weighed in the step (3) to obtain the plant fertilizer.
Compared with the prior art, the plant fertilizer provided by the invention has the following beneficial effects:
1. aspergillus oryzae culture, shell, lettuce leaf, fructus crataegi, bone meal, and Mel as raw materials to provide abundant amino acids, minerals, etc.; the Aspergillus oryzae culture contains abundant amino acids, the shell and bone meal contain abundant calcium elements, the lettuce leaves contain abundant folic acid and vitamins, the hawthorn and the honey contain a large amount of trace elements, and under the combined action of the Aspergillus oryzae and the yeast, nutrient substances in the raw material substances are fully metabolized and decomposed into small molecular substances which are easily absorbed and utilized by plants, so that abundant nutrition is provided for the plants.
2. The effective ingredients in the raw materials such as shells and the like are prepared into concentrated plant fertilizer by water extraction and organic solvent extraction, and the concentrated plant fertilizer is diluted by a proper multiple and then sprayed near the root system of a plant as liquid fertilizer, so that the plant can be promoted to grow quickly, and the concentrated plant fertilizer is particularly suitable for the plant which is just transplanted, and helps the plant to adapt to a new environment quickly.
3. The plant fertilizer prepared by the method does not directly contain chemical fertilizers such as potassium fertilizer and the like, is prepared by taking extracts of natural materials as raw materials, can promote plant growth, avoids environmental pollution, and has a simple preparation method and good application prospect.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments. Test methods in which specific conditions are not noted in the following examples are generally performed under conventional conditions or conditions recommended by each manufacturer.
When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In addition to the specific methods, devices, and materials used in the examples, any methods, devices, and materials similar or equivalent to those described in the examples may be used in the practice of the invention in addition to the specific methods, devices, and materials used in the examples, in keeping with the knowledge of one skilled in the art and with the description of the invention.
All the microorganisms used in the examples described below, such as Aspergillus oryzae Hu brewing 3.042, aspergillus oryzae Hu brewing 1.08, and Saccharomyces cerevisiae, were purchased from the microorganism culture Collection, and the Saccharomyces cerevisiae used in the food field for brewing beer was prepared by a conventional method using a wort medium for culturing the yeast.
Example 1
The plant fertilizer is prepared from the following raw materials in parts by weight: 50 parts of aspergillus oryzae culture, 25 parts of shell, 50 parts of lettuce leaf, 15 parts of hawthorn, 10 parts of bone meal, 10 parts of honey, 0.5 part of yeast culture and 0.05 part of 0.6g/100ml sodium chloride solution; the preparation method specifically comprises the following steps:
step 1, preparation of Aspergillus oryzae culture
Step 1.1, activating Aspergillus oryzae strain Huniang 3.042, and eluting Aspergillus oryzae spores by using distilled water to obtain an Aspergillus oryzae spore suspension;
step 1.2, soaking soybeans in water for 4 hours, draining water, then steaming (30-40 min), and cooling to 30-40 ℃ to obtain cooked soybeans; grinding wheat into powder, and sieving with 40 mesh sieve to obtain wheat flour; uniformly mixing the cooked soybean and the wheat flour according to the mass ratio of 1:1 to obtain an aspergillus oryzae culture medium;
step 1.3, inoculating an aspergillus oryzae spore suspension into an aspergillus oryzae culture matrix, wherein 5ml of the aspergillus oryzae spore suspension is inoculated into each kilogram of aspergillus oryzae culture matrix, uniformly mixing, and then culturing at 28-32 ℃ for 2-3 days to obtain an aspergillus oryzae culture;
step 2, preparation of Yeast culture
Inoculating yeast into liquid malt extract culture medium, and culturing at 28-30 deg.C for 2-3d to obtain yeast culture;
step 3, weighing the following components in parts by weight: 50 parts of aspergillus oryzae culture, 25 parts of shell, 50 parts of lettuce leaf, 15 parts of hawthorn, 10 parts of bone meal, 10 parts of honey, 0.5 part of yeast culture and 0.05 part of sodium chloride solution with the concentration of 0.6g/100 ml;
step 4, respectively crushing the shell and the hawthorn weighed in the step 3 to obtain shell powder and hawthorn powder, wherein the shell powder and the hawthorn powder are only required to be crushed into particles, and the particle volume is less than 0.3mm multiplied by 0.3mm; the lettuce She Jiancheng fragments weighed in the step 3 are crushed to obtain lettuce leaf fragments with the area of less than 0.3mm multiplied by 0.3mm;
uniformly mixing shell powder, hawthorn powder, lettuce leaf fragments, the bone meal weighed in the step 3, honey, an aspergillus oryzae culture and a yeast culture, and fermenting for 2d at the temperature of 30 ℃ to obtain a composite fermentation product;
step 5, adding distilled water into the composite fermentation product, enabling the distilled water to permeate the upper surface of the composite fermentation product, continuously stirring for 30min at 50 ℃, filtering, respectively collecting filtrate and filter residue, and evaporating the obtained filtrate to dryness to obtain a water extract;
adding a mixture of dichloromethane and diethyl ether into the obtained filter residue, allowing the mixture of dichloromethane and diethyl ether to permeate the upper surface of the filter residue, wherein the volume ratio of dichloromethane to diethyl ether is 1;
step 6, adding 1mol/L disodium hydrogen phosphate solution into the water extract, wherein the ratio of the aspergillus oryzae culture to the disodium hydrogen phosphate solution is 100g;
adding 1mol/L sodium dihydrogen phosphate solution into the organic solvent extract, wherein the volume of the sodium dihydrogen phosphate solution is the same as that of the disodium hydrogen phosphate solution, and obtaining organic fertilizer solution;
and (4) uniformly mixing the water-soluble fertilizer solution and the organic fertilizer solution, and finally adding 0.6g/100ml of sodium chloride solution weighed in the step (3) to obtain the plant fertilizer.
The content of reducing sugar in the plant fertilizer of example 1 is 4.23g/100ml, the content of total nitrogen is 1.07g/100ml, the content of total free amino acid is more than 1500mg/100ml, wherein the content of aspartic acid, glutamic acid, tyrosine and threonine is more than 100mg/100ml, the content of folic acid is more than 10mg/100ml, and the content of calcium ion is more than 5000mg/100ml.
Example 2
A plant fertilizer is prepared from the following raw materials in parts by weight: 40 parts of aspergillus oryzae culture, 20 parts of shell, 40 parts of lettuce leaf, 10 parts of hawthorn, 8 parts of bone meal, 8 parts of honey, 0.8 part of yeast culture and 0.08 part of gibberellin; the preparation method specifically comprises the following steps:
step 1, preparation of Aspergillus oryzae culture
Step 1.1, activating Aspergillus oryzae strain Huniang 1.08, and eluting Aspergillus oryzae spores by using distilled water to obtain Aspergillus oryzae spore suspension;
step 1.2, soaking soybeans in water for 6 hours, draining water, then steaming (30-40 min), and cooling to 30-40 ℃ to obtain cooked soybeans; grinding wheat into powder, and sieving with 40 mesh sieve to obtain wheat flour; then uniformly mixing the cooked soybeans and the wheat flour according to the mass ratio of 1:1 to obtain an aspergillus oryzae culture medium;
step 1.3, inoculating Aspergillus oryzae spore suspension into an Aspergillus oryzae culture medium, wherein 10ml of Aspergillus oryzae spore suspension is inoculated into each kilogram of Aspergillus oryzae culture medium, uniformly mixing, and then culturing at 28-32 ℃ for 2-3d to obtain an Aspergillus oryzae culture;
step 2, preparation of Yeast culture
Inoculating yeast into liquid malt extract culture medium, and culturing at 28-30 deg.C for 2-3d to obtain yeast culture;
step 3, weighing the following components in parts by weight: 40 parts of aspergillus oryzae culture, 20 parts of shell, 40 parts of lettuce leaf, 10 parts of hawthorn, 8 parts of bone meal, 8 parts of honey, 0.8 part of yeast culture and 0.08 part of gibberellin;
step 4, respectively crushing the shell and the hawthorn weighed in the step 3 to obtain shell powder and hawthorn powder, wherein the shell powder and the hawthorn powder are only required to be crushed into particles, and the particle volume is less than 0.3mm multiplied by 0.3mm; the lettuce She Jiancheng fragments weighed in the step 3 are crushed to obtain lettuce leaf fragments with the area smaller than 0.3mm multiplied by 0.3mm;
uniformly mixing shell powder, hawthorn powder, lettuce leaf fragments, the bone meal, honey, an aspergillus oryzae culture and a yeast culture weighed in the step (3), and fermenting for 2d at the temperature of 30 ℃ to obtain a composite fermented product;
step 5, adding distilled water into the composite fermentation product, enabling the distilled water to flow over the upper surface of the composite fermentation product, continuously stirring for 30min at 50 ℃, filtering, respectively collecting filtrate and filter residue, and evaporating the obtained filtrate to dryness to obtain a water extract;
adding a mixture of dichloromethane and diethyl ether into the obtained filter residue, allowing the mixture of dichloromethane and diethyl ether to permeate the upper surface of the filter residue, wherein the volume ratio of dichloromethane to diethyl ether is 1;
step 6, adding 1mol/L disodium hydrogen phosphate solution into the water extract, wherein the ratio of the aspergillus oryzae culture to the disodium hydrogen phosphate solution is 100g;
adding 1mol/L sodium dihydrogen phosphate solution into the organic solvent extract, wherein the volume of the sodium dihydrogen phosphate solution is the same as that of the disodium hydrogen phosphate solution, and obtaining organic fertilizer solution;
and (3) uniformly mixing the water-soluble fertilizer solution and the organic fertilizer solution, and finally adding the gibberellin weighed in the step (3) to obtain the plant fertilizer.
The content of reducing sugar in the plant fertilizer of the embodiment 2 is 4.60g/100ml, the content of total nitrogen is 1.12g/100ml, the content of total free amino acid is more than 1500mg/100ml, wherein the content of aspartic acid, glutamic acid, tyrosine and threonine is more than 100mg/100ml, the content of folic acid is more than 10mg/100ml, and the content of calcium ion is more than 5000mg/100ml.
Example 3
A plant fertilizer is prepared from the following raw materials in parts by weight: 60g of aspergillus oryzae culture, 30g of shell, 60g of lettuce leaf, 20g of hawthorn, 5g of bone meal, 5g of honey, 1g of yeast culture, 0.05g of ethephon and 0.05g of naphthylacetic acid; the preparation method specifically comprises the following steps:
step 1, preparation of Aspergillus oryzae culture
Step 1.1, activating Aspergillus oryzae strain Huniang 3.042, and eluting Aspergillus oryzae spores by using distilled water to obtain an Aspergillus oryzae spore suspension;
step 1.2, soaking soybeans in water for 5 hours, draining water, then steaming (30-40 min), and cooling to 30-40 ℃ to obtain cooked soybeans; grinding wheat into powder, and sieving with 40 mesh sieve to obtain wheat flour; then uniformly mixing the cooked soybeans and the wheat flour according to the mass ratio of 1:1 to obtain an aspergillus oryzae culture medium;
step 1.3, inoculating an Aspergillus oryzae spore suspension into an Aspergillus oryzae culture medium, wherein 8ml of the Aspergillus oryzae spore suspension is inoculated into each kilogram of Aspergillus oryzae culture medium, uniformly mixing, and then culturing at 28-32 ℃ for 2-3d to obtain an Aspergillus oryzae culture;
step 2, preparation of Yeast culture
Inoculating yeast into liquid wort culture medium, and culturing at 28-30 deg.C for 2-3d to obtain yeast culture;
step 3, weighing the following components in parts by weight: 60g of aspergillus oryzae culture, 30g of shell, 60g of lettuce leaf, 20g of hawthorn, 5g of bone meal, 5g of honey, 1g of saccharomycete culture, 0.05g of ethephon and 0.05g of naphthylacetic acid;
step 4, respectively crushing the shell and the hawthorn weighed in the step 3 to obtain shell powder and hawthorn powder, wherein the shell powder and the hawthorn powder are only required to be crushed into particles, and the particle volume is less than 0.3mm multiplied by 0.3mm; the lettuce She Jiancheng fragments weighed in the step 3 are crushed to obtain lettuce leaf fragments with the area smaller than 0.3mm multiplied by 0.3mm;
uniformly mixing shell powder, hawthorn powder, lettuce leaf fragments, the bone meal weighed in the step 3, honey, an aspergillus oryzae culture and a yeast culture, and fermenting for 2d at the temperature of 30 ℃ to obtain a composite fermentation product;
step 5, adding distilled water into the composite fermentation product, enabling the distilled water to permeate the upper surface of the composite fermentation product, continuously stirring for 30min at 50 ℃, filtering, respectively collecting filtrate and filter residue, and evaporating the obtained filtrate to dryness to obtain a water extract;
adding a mixture of dichloromethane and diethyl ether into the obtained filter residue, allowing the mixture of dichloromethane and diethyl ether to permeate the upper surface of the filter residue, wherein the volume ratio of dichloromethane to diethyl ether is 1;
step 6, adding 3ml of 1mol/L disodium hydrogen phosphate solution into the water extract to obtain an aqueous fertilizer solution;
adding 3ml of 1mol/L sodium dihydrogen phosphate solution into the organic solvent extract to obtain organic fertilizer solution;
and (3) uniformly mixing the water-soluble fertilizer solution and the organic fertilizer solution, and finally adding the ethephon and the naphthylacetic acid weighed in the step (3) to obtain the plant fertilizer.
The content of reducing sugar in the plant fertilizer of the embodiment 3 is 4.45g/100ml, the content of total nitrogen is 1.03g/100ml, the content of total free amino acid is more than 1500mg/100ml, wherein the content of aspartic acid, glutamic acid, tyrosine and threonine is more than 100mg/100ml, the content of folic acid is more than 10mg/100ml, and the content of calcium ion is more than 5000mg/100ml.
The plant fertilizer disclosed by the invention is diluted by 100-1000 times according to actual needs for use, can be used as a liquid fertilizer to be sprayed near a plant root system, can promote the rapid growth of plants, is suitable for the plants which are just transplanted, and helps the plants to adapt to a new environment rapidly.
Diluting the plant fertilizer of the embodiment 1-3 by 200 times, and spraying the diluted plant fertilizer into newly transplanted flowers such as narcissus and peony, wherein the plants can grow normally in 1 day; chemical fertilizers such as urea, ammonium bicarbonate and the like are applied for 3 days, and then the growth can be performed normally; and the normal growth can be recovered after 5 days without using any fertilizer.
The plant fertilizer of the embodiment 1-3 is diluted by 300 times and sprayed in the newly-cut Chinese rose, the plant fertilizer of the embodiment 1 can germinate in 8 days, the plant fertilizer of the embodiment 2 can germinate in 7 days, and the plant fertilizer of the embodiment 3 can germinate in 7 days; under the same conditions, the Chinese roses without the plant fertilizer can germinate in 15 days, which shows that the plant fertilizer can effectively promote the growth of plants and shorten the growth and germination time of the plants.
The plant fertilizer of the embodiment 1-3 is diluted by 400 times and then sprayed to the vicinity of root systems of plants with poor growth vigor, such as rohdea japonica, pachira macrocarpa, peony, asparagus fern, narcissus, and the like, and the normal growth state can be recovered within 2 days, the vigorous growth vigor is ensured, and the rapid growth of the plants can be promoted.
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (4)
1. The manufacturing method of the plant fertilizer is characterized by comprising the following steps:
step 1, preparation of Aspergillus oryzae culture
Step 1.1, activating aspergillus oryzae strains, and eluting aspergillus oryzae spores by using distilled water to obtain an aspergillus oryzae spore suspension;
step 1.2, soaking soybeans in water for 4-6h, draining, steaming, and cooling to 30-40 ℃ to obtain cooked soybeans; grinding wheat into powder, and sieving with 40 mesh sieve to obtain wheat flour; then uniformly mixing the cooked soybeans and the wheat flour according to the mass ratio of 1:1 to obtain an aspergillus oryzae culture medium;
step 1.3, inoculating an Aspergillus oryzae spore suspension into an Aspergillus oryzae culture medium, wherein 5-10ml of the Aspergillus oryzae spore suspension is inoculated to each kilogram of Aspergillus oryzae culture medium, uniformly mixing, and then culturing at 28-32 ℃ for 2-3d to obtain an Aspergillus oryzae culture;
step 2, preparation of Yeast culture
Inoculating yeast into liquid malt extract culture medium, and culturing at 28-30 deg.C for 2-3d to obtain yeast culture;
step 3, weighing the following components in parts by weight: 40-60 parts of aspergillus oryzae culture, 20-30 parts of shell, 40-60 parts of lettuce leaf, 10-20 parts of hawthorn, 5-10 parts of bone meal, 5-10 parts of honey, 0.5-1 part of yeast culture and 0.05-0.1 part of growth regulator;
step 4, respectively crushing the shell and the hawthorn weighed in the step 3 to obtain shell powder and hawthorn powder; the lettuce She Jiancheng fragments weighed in the step 3 are crushed to obtain lettuce leaf fragments;
uniformly mixing shell powder, hawthorn powder, lettuce leaf fragments, the bone meal weighed in the step 3, honey, an aspergillus oryzae culture and a yeast culture, and fermenting for 2d at the temperature of 30 ℃ to obtain a composite fermentation product;
step 5, adding distilled water into the composite fermentation product, continuously stirring for 30min at 50 ℃, filtering, respectively collecting filtrate and filter residue, and evaporating the obtained filtrate to dryness to obtain a water extract;
adding a mixture of dichloromethane and diethyl ether into the obtained filter residue, wherein the volume ratio of dichloromethane to diethyl ether is 1;
step 6, adding 1mol/L disodium hydrogen phosphate solution into the water extract, wherein the ratio of the aspergillus oryzae culture to the disodium hydrogen phosphate solution is 100g;
adding 1mol/L sodium dihydrogen phosphate solution into the organic solvent extract, wherein the volume of the sodium dihydrogen phosphate solution is the same as that of the disodium hydrogen phosphate solution, and obtaining organic fertilizer solution;
uniformly mixing the water-soluble fertilizer solution and the organic fertilizer solution, and finally adding the growth regulator weighed in the step (3) to obtain a plant fertilizer;
the growth regulator is one or more of gibberellin, ethephon, naphthylacetic acid, and 0.6g/100ml sodium chloride solution.
2. The method as claimed in claim 1, wherein the Aspergillus oryzae is Aspergillus oryzae Hu brewing 3.042 or Aspergillus oryzae Hu brewing 1.08.
3. The method for preparing a plant fertilizer as claimed in claim 1, wherein the yeast is Saccharomyces cerevisiae.
4. The method for preparing the plant fertilizer according to claim 1, which is characterized by being prepared from the following raw materials in parts by weight: 50 parts of aspergillus oryzae culture, 25 parts of shell, 50 parts of lettuce leaf, 15 parts of hawthorn, 10 parts of bone meal, 10 parts of honey, 0.5 part of yeast culture and 0.05 part of growth regulator.
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| CN1243816A (en) * | 1999-08-10 | 2000-02-09 | 山西省农业科学院棉花研究所 | Rapid fermenting process for preparing multi-element liquid fertilizer of amino acids |
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| CN1243816A (en) * | 1999-08-10 | 2000-02-09 | 山西省农业科学院棉花研究所 | Rapid fermenting process for preparing multi-element liquid fertilizer of amino acids |
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