CN106614777A - Composition and application thereof in preventing and treating diseases in grafted citrus branches - Google Patents
Composition and application thereof in preventing and treating diseases in grafted citrus branches Download PDFInfo
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- CN106614777A CN106614777A CN201611178781.9A CN201611178781A CN106614777A CN 106614777 A CN106614777 A CN 106614777A CN 201611178781 A CN201611178781 A CN 201611178781A CN 106614777 A CN106614777 A CN 106614777A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
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- Plant Pathology (AREA)
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Abstract
The invention provides a composition, prepared from 25-35 parts by weight of herb and seed of foxtail-like sophora, 15-25 parts by weight of castor bean leaf and root, 25-35 parts by weight of bitter skin cane, 8-12 parts by weight of herb and seed of Asiatic plantain, and 8-12 parts by weight of Chinese stellera root or fischer euphorbia root; application of the composition is also provided; a product for preventing and treating diseases in grafted citrus branches is provided. By adding the above herbs and controlling their contents, disease reduction for grafted citrus branches is increased. Experimental results show that the extract of the composition provided herein provides a decrease of 85% in citrus blue mold, a decrease of 87% in citrus brown blotch, a decrease of 65% in citrus canker and a decrease of 75% in citrus anthracnose as compared to a control.
Description
Technical field
The present invention relates to fruit tree disease technical field, more particularly to a kind of compositionss and its in preventing and treating Citrus grafting twig disease
Application in evil.
Background technology
Grafting is the important means of Citrus seedling-wood breeding, and the good and the bad of nursery stock directly affects Citrus Industry health benign development.
Currently, China's Citrus nursery stock production is still more chaotic, in order to cost-effective, shortens young plant cultivation period, simplifies program, so as to
Cause seedling quality inferior.
Need to prevent and treat multiple diseases after Citrus grafting, will otherwise affect the growth of Citrus nursery stock, and then affect Citrus
Yield.Citrus grafting twig disease mainly has Penicillium italicum, brown spot, green mould, citrus ulcer, anthrax, certainly also
There are black heart, shot hole etc..At present, the method that Citrus grafting twig typically takes chemical prevention in production, does not even make diseases prevention
Process, so can not effectively prevent and treat the disease of Citrus grafting twig.
The content of the invention
Present invention solves the technical problem that being to provide a kind of compositionss, the compositionss that the application is provided acquire compositionss
Extract can effectively prevent and treat Citrus grafting twig disease.
In view of this, this application provides a kind of compositionss, are prepared by following raw material：
Preferably, the content of Herba Sophorae alopecuroidiss is 30 weight portions, and the content of Semen Ricini is 20 weight portions, and the content of Celastrus angulatus is 30 weights
Amount part, the content of Herba Plantaginis is 10 weight portions, and the content of the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) is 10 weight portions.
Present invention also provides the preparation method of the compositionss described in such scheme, including：
Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, Herba Plantaginis and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) are crushed, then sequentially passes through sterilizing and drying, then with ethanol water
Solution is that extracting solution is soaking, distills, and collects distillate, obtains compositionss.
Preferably, the fineness of the crushing is 100 mesh.
Preferably, the temperature of the drying is 50～70 DEG C, and the time is 2～3h.
Preferably, the volume fraction of ethanol is 40% in the ethanol water；The Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, car
The ratio of the quality of front gross mass and the ethanol water with the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) is 1:25.
Present invention also provides the group prepared by the preparation method described in the compositionss or such scheme described in such scheme
Application of the compound in the product for preparing preventing and treating Citrus grafting twig disease.
Preferably, the Citrus grafting twig disease includes Penicillium italicum, Citrus brown spot, Citrus brown spot and Citrus
One or more in anthrax.
Present invention also provides it is a kind of preventing and treating Citrus grafting twig disease product, including described in such scheme or it is above-mentioned
The compositionss prepared by preparation method and ethanol solution described in scheme；The compositionss are 1 with the volume ratio of ethanol solution:2,
The ethanol solution is the food-grade ethanol of 50vol%.
This application provides a kind of compositionss, including：The Herba Sophorae alopecuroidiss of 25～35 weight portions, the Semen Ricini of 15～25 weight portions,
The Celastrus angulatus of 25～35 weight portions, the Herba Plantaginis of 8～12 weight portions, the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) of 8～12 weight portions；The system of above-mentioned composition is also provided
Preparation Method and its application；And there is provided a kind of product of preventing and treating Citrus grafting twig disease.The application passes through the above-mentioned medical herbs of addition,
And its content is controlled, make preventing and treating Citrus grafting twig disease effect preferable.Result of the test shows, the compositionss that the present invention is provided
Extract makes Penicillium italicum reduce 85% than control, and Citrus brown spot reduces 87% than control, and citrus ulcer subtracts than control
Few 65%, citrus anthracnose reduces 75% than control.
Specific embodiment
For a further understanding of the present invention, the preferred embodiment of the invention is described with reference to embodiment, but
It should be appreciated that these descriptions are simply to further illustrate the features and advantages of the present invention, rather than to the claims in the present invention
Limit.
The embodiment of the invention discloses a kind of compositionss, are prepared by following raw material：
The application discloses a kind of compositionss with Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, Herba Plantaginis and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) as component, the combination
Thing is used to prevent and treat the disease of Citrus grafting twig and has preferable effect.
Herba Sophorae alopecuroidiss be pulse family Sophora Chinese scholartree kind, scientific name Sophora alopecuroides L.Herba Sophorae alopecuroidiss described herein
Content is 25～35 weight portions, and in certain embodiments, the content of the Herba Sophorae alopecuroidiss is 28～32 weight portions, in some embodiments
In, the content of the Herba Sophorae alopecuroidiss is 30 weight portions.
Semen Ricini (Ricinus communis L.), is Euphorbiaceae Ricinuses.The content of Semen Ricini described herein be 15～
25 weight portions, in certain embodiments, the content of the Semen Ricini is 18～23 weight portions, in certain embodiments, the Semen Ricini
Content be 20 weight portions.
Herba Plantaginis (Plantago asiatica L) are Plantaginaceae Plantago.The content of Herba Plantaginis described herein is 8～12
Weight portion, in certain embodiments, the content of the Herba Plantaginis is 10 weight portions.
Celastrus angulatus is Celastraceae Celastrus, and the content of Celastrus angulatus described herein is 25～35 weight portions, at some
In embodiment, the content of the Celastrus angulatus is 28～32 weight portions, and in certain embodiments, the content of the Celastrus angulatus is 30 weights
Amount part.
The content of the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) described herein is 8～12 weight portions, and in certain embodiments, the content of the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) is 10
Weight portion.
Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, Herba Plantaginis and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) are made compositionss by the application, and mass parts are described in compositionss
The mass parts of Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, Spica Prunellae and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) after drying.Experiment shows, obtains after said composition is extracted
Extract can effectively prevent and treat Citrus grafting twig disease, especially for Penicillium italicum, Citrus brown spot, citrus bacterial canker disease with
Citrus anthracnose.
Present invention also offers the preparation method of above-mentioned composition, the i.e. preparation method of compositionss extract, preparation method
For the way of distillation.Specifically, the preparation method of compositionss of the present invention, including：
After Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, Herba Plantaginis and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) are crushed, then sterilizing and drying are sequentially passed through, then with ethanol
Aqueous solution is that extracting solution is soaking, distills, and collects distillate, obtains compositionss.
During above-mentioned preparation compositionss, the application is first by Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, Herba Plantaginis and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) point
Do not clean, be crushed into 100 mesh post-dryings.The method of the sterilizing is preferably sterilized using irradiation method.
After sterilization, the application dries in the medical herbs after sterilizing.In embodiment, the temperature of the drying is preferably 50
～70 DEG C, the time of the drying is preferably 2～3h.The volume fraction of ethanol is 40% in the ethanol water；The hardship
The ratio of the quality of the gross mass of bean or pea, Semen Ricini, Celastrus angulatus, Herba Plantaginis and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) and the ethanol water is preferably 1:25.This
In the ethanol water that application is adopted, ethanol is food-grade ethanol.The temperature of the distillation be 80～120 DEG C, the time be 2～
5h.The time of the immersion is 2～4h, and in certain embodiments, the time of the immersion is 3h.
Present invention also provides the compositionss or prepared compositionss described in such scheme are preparing preventing and treating Citrus grafting
Application in the product of branch disease.
The disease of herein described Citrus grafting twig is primarily directed to Penicillium italicum, Citrus brown spot, Citrus and bursts
One or more of infectionss disease and citrus anthracnose.
Present invention also provides a kind of product of sterilization Citrus grafting twig, including the compositionss and second described in such scheme
Alcoholic solution；The compositionss are 1 with the volume ratio of ethanol solution:2, the ethanol solution is the food-grade ethanol of 50vol%.
According to the present invention, using the said goods sterilization Citrus grafting twig, specifically, the said goods are mixed with distilled water
Close, the said goods are 1 with the volume ratio of distilled water:10；Citrus grafting scion is processed into 10min using the said goods, while adopting
The said goods are stained with disposable cotton swab process stock otch.
This application provides compositionss, its preparation method including Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, Herba Plantaginis and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) with should
With；A kind of product of preventing and treating Citrus grafting twig disease is provided simultaneously.Result of the test shows that Penicillium italicum is reduced than control
85%, Citrus brown spot reduces 87% than control, and citrus ulcer reduces 65% than control, and citrus anthracnose is reduced than control
75%.
For a further understanding of the present invention, the compositionss provided the present invention with reference to embodiment and its for preventing and treating Citrus chachiensis Hort.
Fructus Citri tangerinae grafting twig disease is described in detail, and protection scope of the present invention is not limited by the following examples.
Raw material in following examples is commercially available prod.
The preparation of the extract of embodiment 1
Get the raw materials ready：Fresh Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, Herba Plantaginis and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) are respectively washed, 100 mesh after bake are crushed into
It is dry standby；
Proportioning：By the Herba Sophorae alopecuroidiss 300g after drying, Semen Ricini 200g, Celastrus angulatus 300g, Herba Plantaginis 100g, Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) 100g, mix；
Sterilizing：Irradiation method sterilizes；
Batch mixing：Heating, drying 2～3 hours at 50～70 DEG C, compositionss；
Distillation：The compositionss are placed in distilling apparatus, the ethanol for adding 25kg volume fraction of ethanol to be 40% is water-soluble
Liquid, soaks 3 hours, then distills 2～5h in 80～120 DEG C, collects distillate, is extract.
The preparation of the extract of embodiment 2
Get the raw materials ready：Fresh Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, Herba Plantaginis and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) are respectively washed, 100 mesh after bake are crushed into
It is dry standby；
Proportioning：By the Herba Sophorae alopecuroidiss 350g after drying, Semen Ricini 150g, Celastrus angulatus 250g, Herba Plantaginis 120g, Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) 80g, mix；
Sterilizing：Irradiation method sterilizes；
Batch mixing：Heating, drying 2～3 hours at 50～70 DEG C, compositionss；
Distillation：The compositionss are placed in distilling apparatus, the ethanol water that 23.75kg volume fraction of ethanol is 40% is added
Solution, soaks 3 hours, then distills 2～5h in 80～120 DEG C, collects distillate, is extract.
The preparation of the extract of embodiment 3
Get the raw materials ready：Fresh Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, Herba Plantaginis and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) are respectively washed, 100 mesh after bake are crushed into
It is dry standby；
Proportioning：By the Herba Sophorae alopecuroidiss 250g after drying, Semen Ricini 250g, Celastrus angulatus 350g, Herba Plantaginis 80g, Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) 120g, mix.
Sterilizing：Irradiation method sterilizes；
Batch mixing：Heating, drying 2～3 hours at 50～70 DEG C, compositionss；
Distillation：The compositionss are placed in distilling apparatus, the ethanol water that 26.26kg volume fraction of ethanol is 40% is added
Solution, soaks 3 hours, then distills 2～5h in 80～120 DEG C, collects distillate, is extract.
Embodiment 4 prevents and treats the product of Citrus grafting twig disease
Extract 1L obtained in Example 1, with the ethanol solution that 2L volume fraction of ethanol is 50% preventing and treating is mixed to prepare
The product of Citrus grafting twig disease.
The product 1L for taking above-mentioned preparation mixes with 10L distilled waters, and the finished product of preventing and treating Citrus grafting twig disease is obtained, convenient
Use.
Embodiment 5 prevents and treats the product of Citrus grafting twig disease
Extract 1L obtained in Example 2, with the ethanol solution that 2L volume fraction of ethanol is 50% preventing and treating is mixed to prepare
The product of Citrus grafting twig disease.
The product 1L for taking above-mentioned preparation mixes with 10L distilled waters, and the finished product of preventing and treating Citrus grafting twig disease is obtained, convenient
Use.
Embodiment 6 prevents and treats the product of Citrus grafting twig disease
Extract 1L obtained in Example 3, with the ethanol solution that 2L volume fraction of ethanol is 50% preventing and treating is mixed to prepare
The product of Citrus grafting twig disease.
The product 1L for taking above-mentioned preparation mixes with 10L distilled waters, and the finished product of preventing and treating Citrus grafting twig disease is obtained, convenient
Use.
Embodiment 7
Late March-mid-April, chooses 2 pieces consistent of field of warm light liquid manure soil, and area is 0.1 mu；It is identical
Scion and identical stock；1 piece of Citrus grafting scion is processed 10 minutes with the disinfectant solution, while being stained with sterilization with disposable cotton swab
Liquid processes stock otch, and 1 piece of Citrus scion in addition is processed 10 minutes with weight distilled water, while being stained with double steamings with disposable cotton swab
Water process stock otch.Experiment sets experimental group and matched group and blank group.Wherein：
The product of preventing and treating Citrus grafting twig disease prepared by the spraying embodiment 4 of experimental group 1；
The product of preventing and treating Citrus grafting twig disease prepared by the spraying embodiment 5 of experimental group 2；
The product of preventing and treating Citrus grafting twig disease prepared by the spraying embodiment 6 of experimental group 3；
Matched group is same weight distilled water；
Each group test is repeated 3 times.Experimental result is as shown in table 1～3.
The experimental group of table 1 prevents and treats the tables of data of Penicillium italicum with matched group
The experimental group of table 2 prevents and treats the tables of data of Citrus brown spot with matched group
The experimental group of table 3 prevents and treats the tables of data of Citrus chachiensis Hort. ulcer pinta with matched group
The experimental group of table 4 prevents and treats the tables of data of citrus anthracnose with matched group
The experimental group of table 5 prevents and treats the tables of data of Penicillium italicum with matched group
The experimental group of table 6 prevents and treats the tables of data of Citrus brown spot with matched group
The experimental group of table 7 prevents and treats the tables of data of citrus bacterial canker disease with matched group
The experimental group of table 8 prevents and treats the tables of data of citrus anthracnose with matched group
The experimental group of table 9 prevents and treats the tables of data of Penicillium italicum with matched group
The experimental group of table 10 prevents and treats the tables of data of Citrus brown spot with matched group
The experimental group of table 11 prevents and treats the tables of data of citrus bacterial canker disease with matched group
The experimental group of table 12 prevents and treats the tables of data of citrus anthracnose with matched group
The explanation of above example is only intended to help and understands the method for the present invention and its core concept.It should be pointed out that right
For those skilled in the art, under the premise without departing from the principles of the invention, the present invention can also be carried out
Some improvement and modification, these are improved and modification is also fallen in the protection domain of the claims in the present invention.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or using the present invention.
Various modifications to these embodiments will be apparent for those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, the present invention
The embodiments shown herein is not intended to be limited to, and is to fit to and principles disclosed herein and features of novelty phase one
The most wide scope for causing.
Claims (9)
1. a kind of compositionss, it is characterised in that prepared by following raw material：
2. compositionss according to claim 1, it is characterised in that the content of Herba Sophorae alopecuroidiss is 30 weight portions, the content of Semen Ricini
For 20 weight portions, the content of Celastrus angulatus is 30 weight portions, and the content of Herba Plantaginis is 10 weight portions, and the content of the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) is 10 weight portions.
3. the preparation method of the compositionss described in claim 1, it is characterised in that include：
Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, Herba Plantaginis and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) are crushed, then sequentially passes through sterilizing and drying, then with ethanol water
For extracting solution it is soaking, distillation, collect distillate, obtain compositionss.
4. preparation method according to claim 3, it is characterised in that the fineness of the crushing is 100 mesh.
5. preparation method according to claim 3, it is characterised in that the temperature of the drying is 50～70 DEG C, and the time is 2
～3h.
6. preparation method according to claim 3, it is characterised in that the volume fraction of ethanol is in the ethanol water
40%；The ratio of the quality of the gross mass of the Herba Sophorae alopecuroidiss, Semen Ricini, Celastrus angulatus, Herba Plantaginis and the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) and the ethanol water is
1:25。
7. prepared by the preparation method described in compositionss described in any one of claim 1～2 or any one of claim 3～6
Compositionss prepare preventing and treating Citrus grafting twig disease product in application.
8. application according to claim 7, it is characterised in that the Citrus grafting twig disease include Penicillium italicum,
One or more in Citrus brown spot, Citrus brown spot and citrus anthracnose.
9. it is a kind of preventing and treating Citrus grafting twig disease product, it is characterised in that including described in any one of claim 1～2
Or the compositionss and ethanol solution prepared by the preparation method described in any one of claim 3～6；The compositionss are molten with ethanol
The volume ratio of liquid is 1:2, the ethanol solution is the food-grade ethanol of 50vol%.
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| 中国土农药志编辑委员会: "《中国土农药志》", 31 May 1959, 科学出版社 * |
| 西南大学柑橘研究所主编: "《柑橘主要病虫害简明识别手册》", 31 January 2013, 中国农业出版社 * |
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