CN106614187A - Straw kneaded filament roll providing shelter for shrimps and crabs and capable of being eaten for same, and manufacturing method for straw kneaded row - Google Patents

Straw kneaded filament roll providing shelter for shrimps and crabs and capable of being eaten for same, and manufacturing method for straw kneaded row Download PDF

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CN106614187A
CN106614187A CN201611180878.3A CN201611180878A CN106614187A CN 106614187 A CN106614187 A CN 106614187A CN 201611180878 A CN201611180878 A CN 201611180878A CN 106614187 A CN106614187 A CN 106614187A
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straw
clusters
kneaded
white
rapeseed cake
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蒋杰
陈开健
唐英
张琴
刘小燕
蒋常青
戴振炎
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Xiangyin Crane Longhu Jie Sheng Ecological Agriculture Development Co Ltd
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Xiangyin Crane Longhu Jie Sheng Ecological Agriculture Development Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a straw kneaded filament roll providing a shelter for shrimps and crabs and capable of being eaten for the same, and a manufacturing method for the straw kneaded roll. The straw kneaded filament roll is composed of the following components according to a mass percentage: 76.5% to 85.5% of straw kneaded filamenets, 8.5% to 9.5% of vegetable cakes and 5% to 15% of white-rot fungi and green trichoderma fungi mixed fungi solution. The straws are fresh crop straws; kneaded filaments are fully mixed with the vegetable cakes, and then fermented for 10 to 15 days via the white-rot fungi and green trichoderma fungi mixed fungi solution; the mass proportion of the straw kneaded filaments to the vegetable cakes is 9 to 1; the straw kneaded filaments can work as a shelter for the shrimps and the crabs, and can also work as nutrients eaten by the shrimps and the crabs; and the straw kneaded filaments fermented via the white-rot fungi and green trichoderma fungi mixed fungi solution having great decomposing effect on fibers can be easily digested and absorbed, so nutrition effect can be played.

Description

Straw Kneading clusters that a kind of confession shrimp crab is hidden and can be ingested and preparation method thereof
Technical field
The present invention relates to technical field of aquaculture, the straw rubbing that more particularly to a kind of confession shrimp crab is hidden and can be ingested Group and preparation method thereof.
Background technology
River crab and freshwater crayfish are name spy's aquatic products of China's breeding prospect great in the last few years, wherein paddy field aquaculture mould Formula and pond culture pattern are all relatively universal and ripe patterns.This both of which emphasizes the importance of a kind grass i.e.:" shrimp (crab) is how many, sees pasture and water ", " support shrimp (crab) and first support grass ".Hide and breathe out to be taken the photograph by it because grass plantation road grows into offer shrimp crab enough Food, need the regular hour, in shrimp crab puts into rice field or pond after cannot can be enough to for some time hide for it Where and the food ingested and cause it larger death occur.
According to relevant report, in the world annual about 2,000,000,000 tons stalk resource from plantation various cereal crops, but The part being wherein utilized only accounts for 10% or so, it may be said that most of also undressed utilization.Shi Ge large agricultural countries of China, possess big All kinds of agricultural straw resources of amount, its occupancy volume occupies first place in the world.According to estimates, all kinds of agricultural crop straw gross annual output amounts of China are just Up to more than 600,000,000 tons, wherein, hundred million tons of straw 2-3 occupies first place.And China's straw utilization rate is about 33%, wherein most without Working process, what is utilized after technical finesse only accounts for 2.6%.The stalk resources such as rural area paddy rice are completely in high consumption, height Pollution, the situation of low output, major part is incinerated in field, does not obtain rational exploitation and utilization.Simultaneously stalk is in combustion process In can produce the pollutants such as volume of smoke, particulate matter and carbon dioxide, carbon monoxide, and hinder the traveling that highway gets on the car With the landing of aircraft.Agricultural crop straw is converted into into feed, fertilizer, biological products and energy etc. with biotechnology can have The resource that effect is utilized, not only can alleviate resource scarcity pressure, and can avoid because the improper process of agricultural crop straw is made Into problem of environmental pollution, therefore to agricultural crop straw exploitation have important strategic importance and economic benefit.
It can be seen that, there is provided one kind can make full use of stalk material, and hide demand and the desire of ingesting of shrimp crab can be met again Method, it will solve pollution problem of the stalk to environment, but can provide it is cheap hide and material of ingesting, protection to environment and The industry development of shrimp crab is respectively provided with significance.
The content of the invention
For extensive development river crab and the needs of freshwater lobster aquaculture, the present invention provides a kind of hiding for shrimp crab and energy What is be ingested rubs clusters and preparation method thereof.
To solve above-mentioned technical problem, the invention provides a kind of confession shrimp crab hide and can be ingested rub clusters, it is described The each component mass percent of Straw Kneading clusters is respectively:Straw rubbing 76.5-85.5%, rapeseed cake 8.5-9.5%, white-rot fungi With trichoderma viride mixed bacteria liquid 5-15%;The Straw Kneading clusters are that fresh agricultural crop straw is abundant with rapeseed cake Jing after rubbing silk Mixing, then Jing white-rot fungis and the fermentation of trichoderma viride mixed bacteria liquid is made for 10-15 days.
In order that rubbing clusters possesses more preferable form and using effect of ingesting, the proportioning of straw rubbing and rapeseed cake is entered The optimization of one step, further, straw rubbing is 9: 1 with the mass ratio of rapeseed cake.
Wherein, in white-rot fungi and trichoderma viride mixed bacteria liquid, both ratios are 1: 1.
In view of shrimp crab cultivation optimum density, it is ensured that rub making full use of for clusters, it is preferable that the soft clusters of stalk it is concrete Put-on method is:It is rendered to the shoal in rice field and pond according to 0.5-1 kg/acre of ratio, or by its fraction Be placed at the deep water in rice field or pond in mud.
Present invention also offers the preparation method for rubbing clusters that a kind of above-mentioned confession shrimp crab is hidden and can be ingested, under it includes Row step:
1), the stalk of fresh harvesting is removed after silt, debris, Jing wire kneading machines are processed into straw rubbing;
2), rapeseed cake is equably crushed, then according to certain mass ratio takes straw rubbing and broken rapeseed cake is placed in The ground of one cleaning, is sufficiently stirred for, and is well mixed standby;
3), the white-rot fungi and trichoderma viride bacterial classification of 4 DEG C Celsius refrigeration are inoculated in into respectively fresh PDA slant medium On, after 28 DEG C of incubated 5d;Take by mass percentage wheat bran 2%, glucose 2%, beancake powder 2%, sucrose 1%, (NH4)2SO40.5%th, KH2PO40.2%th, MgSO4·7H2O 0.1%, dusty yeast 0.2%, balance of water, pH natures, are configured to Seed liquid culture medium;With oese from PDA slant mediums first picking 1ml white-rot fungis spore inoculating to equipped with 100mL kinds In the 250mL shaking flasks of sub- liquid culture medium, 4d is cultivated on 28 DEG C, the constant-temperature table of 150r/min, then with oese from PDA inclined-planes Picking 1ml trichoderma virides spore continues common training in the shaking flask on 28 DEG C, the constant-temperature table of 150r/min on culture medium Foster 4d, obtains final product white-rot fungi and trichoderma viride mixed bacteria liquid;
4), step 2 is taken by mass percentage) straw rubbing that obtains and rapeseed cake mixture 95%, glucose 2.5%, beans Cake powder 2%, KH2PO40.4%th, MgSO4.7H2O 0.1% is hybridly prepared into solid-state fermentation culture medium, and by step 3) obtain White-rot fungi and trichoderma viride mixed bacteria liquid are inoculated in solid-state fermentation culture medium, and inoculum concentration is straw in solid-state fermentation culture medium Stalk rubs the 1/19-3/17 of silk and rapeseed cake mixture quality, while add water adjusting to solid-to-liquid ratio 1: 1.2-1.4, straw is obtained after shaking up Stalk rubs silk, rapeseed cake and bacterium solution mixture, and mixes thoroughly per 3 days and overturn once, and fermentation temperature is 28-30 DEG C, fermentation time 5-10 My god, obtain final product Straw Kneading clusters.
As a preferred version of Straw Kneading clusters preparation method of the present invention, step 2) according to 9: 1 mass ratio will Straw rubbing is mixed with rapeseed cake.
The present invention rubs clusters using what straw rubbing was fabricated for that shrimp crab hides and can be ingested, and straw rubbing both can conduct Shrimp crab is hidden place, it is also possible to as nutriment be to be ingested by shrimp crab, and Jing has the white rot of fine degradation true to fiber Straw rubbing after bacterium and the fermentation of trichoderma viride mixed bacteria liquid is more easy to digested and is ingested so as to play trophism.Cause This, both good place of hiding, and energy were provided using straw rubbing as the clusters of rubbing hidden for shrimp crab and can be ingested as shrimp crab Safety utilizes stalk resource ecologically, reduces the pollution to environment and becomes pollution into resource.
Specific embodiment
Following examples illustrate in greater detail the present invention, but following embodiments the invention is not limited in any way.
Embodiment 1:
The each component mass percent of Straw Kneading clusters is respectively:Straw rubbing 81%, rapeseed cake 9%, white-rot fungi and green Color Trichoderma mixed bacteria liquid 10%;
1), the stalk of fresh harvesting is removed after silt, debris, Jing wire kneading machines are processed into straw rubbing;
2), rapeseed cake is equably crushed, then according to 9: 1 mass ratios take straw rubbing and broken rapeseed cake is placed in one Clean ground, is sufficiently stirred for, and is well mixed standby;
3), the white-rot fungi and trichoderma viride bacterial classification of 4 DEG C Celsius refrigeration are inoculated in into respectively fresh PDA slant medium On, after 28 DEG C of incubated 5d;Take by mass percentage wheat bran 2%, glucose 2%, beancake powder 2%, sucrose 1%, (NH4)2SO40.5%th, KH2PO40.2%th, MgSO4·7H2O 0.1%, dusty yeast 0.2%, balance of water, pH natures, are configured to Seed liquid culture medium;With oese from PDA slant mediums first picking 1ml white-rot fungis spore inoculating to equipped with 100mL kinds In the 250mL shaking flasks of sub- liquid culture medium, 4d is cultivated on 28 DEG C, the constant-temperature table of 150r/min, then with oese from PDA inclined-planes Picking 1ml trichoderma virides spore continues common training in the shaking flask on 28 DEG C, the constant-temperature table of 150r/min on culture medium Foster 4d, obtains final product white-rot fungi and trichoderma viride mixed bacteria liquid;
4), step 2 is taken by mass percentage) straw rubbing that obtains and rapeseed cake mixture 95%, glucose 2.5%, beans Cake powder 2%, KH2PO40.4%th, MgSO4.7H2O 0.1% is hybridly prepared into solid-state fermentation culture medium, and by step 3) obtain White-rot fungi and trichoderma viride mixed bacteria liquid are inoculated in solid-state fermentation culture medium, and inoculum concentration is straw in solid-state fermentation culture medium Stalk rubs the 1/9 of silk and rapeseed cake mixture quality, while add water adjusting to solid-to-liquid ratio 1: 1.3, straw rubbing, rapeseed cake is obtained after shaking up And bacterium solution mixture, and mixed thoroughly per 3 days and overturn once, fermentation temperature is 28-30 DEG C, and fermentation time 5-10 days obtains final product stalk Rub clusters.
Embodiment 2:
The each component mass percent of Straw Kneading clusters is respectively:Straw rubbing 76.5%, rapeseed cake 8.5%, white-rot fungi With trichoderma viride mixed bacteria liquid 15%;
1) straw rubbing:By the herbage stalk of fresh harvesting except again Jing wire kneading machines are processed into straw rubbing after silt, debris.
2) straw rubbing uniformly mixes with rapeseed cake:Rapeseed cake is equably crushed, then according to 9: 1 mass ratio takes stalk Rub silk and rapeseed cake is placed in the ground of a cleaning, be thoroughly mixed straw rubbing and rapeseed cake with shovel uniform standby.
3), the white-rot fungi and trichoderma viride bacterial classification of 4 DEG C Celsius refrigeration are inoculated in into respectively fresh PDA slant medium On, after 28 DEG C of incubated 5d;Take by mass percentage wheat bran 2%, glucose 2%, beancake powder 2%, sucrose 1%, (NH4)2SO40.5%th, KH2PO40.2%th, MgSO4·7H2O 0.1%, dusty yeast 0.2%, balance of water, pH natures, are configured to Seed liquid culture medium;With oese from PDA slant mediums first picking 1ml white-rot fungis spore inoculating to equipped with 100mL kinds In the 250mL shaking flasks of sub- liquid culture medium, 4d is cultivated on 28 DEG C, the constant-temperature table of 150r/min, then with oese from PDA inclined-planes Picking 1ml trichoderma virides spore continues common training in the shaking flask on 28 DEG C, the constant-temperature table of 150r/min on culture medium Foster 4d, obtains final product white-rot fungi and trichoderma viride mixed bacteria liquid;
4), step 3 is taken by mass percentage) straw rubbing that obtains and rapeseed cake mixture 95%, glucose 2.5%, beans Cake powder 2%, KH2PO40.4%th, MgSO4.7H2O 0.1% is hybridly prepared into solid-state fermentation culture medium, and by step 3) obtain White-rot fungi and trichoderma viride mixed bacteria liquid are inoculated in solid-state fermentation culture medium, and inoculum concentration is straw in solid-state fermentation culture medium Stalk rubs the 3/17 of silk and rapeseed cake mixture quality, while add water adjusting to solid-to-liquid ratio 1: 1.4, straw rubbing, dish is obtained after shaking up Cake and bacterium solution mixture, and mixed thoroughly per 3 days and overturn once, fermentation temperature is 28-30 DEG C, and fermentation time 5-10 days obtains final product straw Stalk rubs clusters.
Embodiment 3
The each component mass percent of Straw Kneading clusters is respectively:Straw rubbing 85.5%, rapeseed cake 9.5%, white-rot fungi With trichoderma viride mixed bacteria liquid 5%;
1) straw rubbing:By the herbage stalk of fresh harvesting except again Jing wire kneading machines are processed into straw rubbing after silt, debris.
2) straw rubbing uniformly mixes with rapeseed cake:Rapeseed cake is equably crushed, then according to 9: 1 mass ratio takes stalk Rub silk and rapeseed cake is placed in the ground of a cleaning, be thoroughly mixed straw rubbing and rapeseed cake with shovel uniform standby.
3), the white-rot fungi and trichoderma viride bacterial classification of 4 DEG C Celsius refrigeration are inoculated in into respectively fresh PDA slant medium On, after 28 DEG C of incubated 5d;Take by mass percentage wheat bran 2%, glucose 2%, beancake powder 2%, sucrose 1%, (NH4)2SO40.5%th, KH2PO40.2%th, MgSO4·7H2O 0.1%, dusty yeast 0.2%, balance of water, pH natures, are configured to Seed liquid culture medium;With oese from PDA slant mediums first picking 1ml white-rot fungis spore inoculating to equipped with 100mL kinds In the 250mL shaking flasks of sub- liquid culture medium, 4d is cultivated on 28 DEG C, the constant-temperature table of 150r/min, then with oese from PDA inclined-planes Picking 1ml trichoderma virides spore continues common training in the shaking flask on 28 DEG C, the constant-temperature table of 150r/min on culture medium Foster 4d, obtains final product white-rot fungi and trichoderma viride mixed bacteria liquid;
4), step 3 is taken by mass percentage) straw rubbing that obtains and rapeseed cake mixture 95%, glucose 2.5%, beans Cake powder 2%, KH2PO40.4%th, MgSO4.7H2O 0.1% is hybridly prepared into solid-state fermentation culture medium, and by step 3) obtain White-rot fungi and trichoderma viride mixed bacteria liquid are inoculated in solid-state fermentation culture medium, and inoculum concentration is straw in solid-state fermentation culture medium Stalk rubs the 1/19 of silk and rapeseed cake mixture quality, while add water adjusting to solid-to-liquid ratio 1: 1.2, straw rubbing, dish is obtained after shaking up Cake and bacterium solution mixture, and mixed thoroughly per 3 days and overturn once, fermentation temperature is 28-30 DEG C, and fermentation time 5-10 days obtains final product straw Stalk rubs clusters.

Claims (6)

1. a kind of Straw Kneading clusters that confession shrimp crab is hidden and can be ingested, it is characterised in that each component of the Straw Kneading clusters Mass percent is respectively:Straw rubbing 76.5-85.5%, rapeseed cake 8.5-9.5%, white-rot fungi and trichoderma viride Mixed Microbes Liquid 5-15%;The Straw Kneading clusters are to be sufficiently mixed fresh agricultural crop straw with rapeseed cake Jing after rubbing silk, and then Jing white rots are true Bacterium and the fermentation of trichoderma viride mixed bacteria liquid are made for 10-15 days.
2. the Straw Kneading clusters that a kind of confession shrimp crab according to claim 1 is hidden and can be ingested, it is characterised in that described Straw rubbing is 9: 1 with the mass ratio of rapeseed cake.
3. the Straw Kneading clusters that a kind of confession shrimp crab according to claim 1 is hidden and can be ingested, it is characterised in that described In white-rot fungi and trichoderma viride mixed bacteria liquid, both ratios are 1: 1.
4. the Straw Kneading clusters that a kind of confession shrimp crab according to claim 1 is hidden and can be ingested, it is characterised in that described The concrete put-on method of the soft clusters of stalk is:It is rendered to into the shallow water in rice field and pond according to 0.5-1 kg/acre of ratio Place, either its fraction is placed at the deep water in rice field or pond in mud.
5. a kind of preparation method of the Straw Kneading clusters hidden and can be ingested for shrimp crab as described in any one of claim 1-4, It is characterized in that comprising the following steps:
1), the stalk of fresh harvesting is removed after silt, debris, Jing wire kneading machines are processed into straw rubbing;
2), rapeseed cake is equably crushed, then according to certain mass ratio takes straw rubbing and broken rapeseed cake is placed in one Clean ground, is sufficiently stirred for, and is well mixed standby;
3), the white-rot fungi and trichoderma viride bacterial classification of 4 DEG C Celsius refrigeration are inoculated in respectively on fresh PDA slant medium, After 28 DEG C of incubated 5d;Wheat bran 2%, glucose 2%, beancake powder 2%, sucrose 1%, (NH4) are taken by mass percentage2SO40.5%th, KH2PO40.2%th, MgSO4·7H2O 0.1%, dusty yeast 0.2%, balance of water, pH natures, are configured to seed Liquid culture medium;With oese from PDA slant mediums first picking 1ml white-rot fungis spore inoculating to equipped with 100mL seed liquors In the 250mL shaking flasks of culture medium, 4d is cultivated on 28 DEG C, the constant-temperature table of 150r/min, then with oese from PDA inclined-plane cultures Picking 1ml trichoderma virides spore continues co-incubation in the shaking flask on 28 DEG C, the constant-temperature table of 150r/min on base 4d, obtains final product white-rot fungi and trichoderma viride mixed bacteria liquid;
4), step 2 is taken by mass percentage) straw rubbing that obtains and rapeseed cake mixture 95%, glucose 2.5%, beancake powder 2%th, KH2PO40.4%th, MgSO4.7H2O 0.1% is hybridly prepared into solid-state fermentation culture medium, and by step 3) white rot that obtains Fungi and trichoderma viride mixed bacteria liquid are inoculated in solid-state fermentation culture medium, and inoculum concentration is Straw Kneading in solid-state fermentation culture medium The 1/19-3/17 of silk and rapeseed cake mixture quality, while add water adjusting to solid-to-liquid ratio 1: 1.2-1.4, obtains Straw Kneading after shaking up Silk, rapeseed cake and bacterium solution mixture, and mixed thoroughly per 3 days and overturn once, fermentation temperature is 28-30 DEG C, fermentation time 5-10 days, i.e., Obtain Straw Kneading clusters.
6. the preparation method of Straw Kneading clusters according to claim 6, it is characterised in that step 2) according to 9: 1 matter Amount ratio is mixed straw rubbing with rapeseed cake.
CN201611180878.3A 2016-12-20 2016-12-20 Straw kneaded filament roll providing shelter for shrimps and crabs and capable of being eaten for same, and manufacturing method for straw kneaded row Pending CN106614187A (en)

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CN110859146A (en) * 2019-09-17 2020-03-06 毛健 Crayfish seedling raising method

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CN103960100A (en) * 2014-05-23 2014-08-06 湖北虾乡食品有限公司 Three-dimensional composite duck and shrimp culture method carried out in rice field
CN104509464A (en) * 2014-12-02 2015-04-15 邱汪洋 Paddy field adult crab breeding technical method
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110859146A (en) * 2019-09-17 2020-03-06 毛健 Crayfish seedling raising method

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