CN106613814A - Rapid propagation method of pteridophyte spores - Google Patents
Rapid propagation method of pteridophyte spores Download PDFInfo
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- CN106613814A CN106613814A CN201610869204.8A CN201610869204A CN106613814A CN 106613814 A CN106613814 A CN 106613814A CN 201610869204 A CN201610869204 A CN 201610869204A CN 106613814 A CN106613814 A CN 106613814A
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- spore
- spores
- pteridophyte
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Environmental Sciences (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of pteridophyte spore propagation, and discloses a rapid propagation method of pteridophyte spores. The method comprises the steps of S1, collecting mature spores of a pteridophyte, and removing impurities; S2, preparing MS macroelements, gibberellin, carbendazim and sodium alginate into a gel liquid, and then stirring the gel liquid with the spores evenly to prepare a spore gel liquid; S3, extracting the spore gel liquid, dropwise adding the spore gel liquid into a CaCl2 solution for curing reaction to form spore balls, and taking out and cleaning the spore balls; S4, using peat soil and perlite as a matrix, watering the matrix sufficiently one day before sowing, and a tray is stacked at the bottom for water retention; S5, sowing the spore ball on the surface of the matrix of a plug, wherein one ball is sowed in every plug, there is no need to cover the soil, and a moisture retention cover is covered; S6, maintenance management. According to the rapid propagation method of the pteridophyte spores, fine spores are prepared into artificial seeds, by covering nutrient substances and a bacteriostatic agent, the growth of the spores is accelerated, and thus the spores are grown into mature gametophytes in a short period; meanwhile, the spore density is effectively controlled, transplanting is not needed, and the spores is directly cultivated into seedlings.
Description
Technical field
The invention belongs to pteridophyte raising technology field, specifically a kind of pteridophyta spore quick-breeding method.
Background technology
Pteridophyte is also referred to as pteridophyte, is original vascular plant, is between bryophyte and seed plant
A taxon.It is high cryptogam, with obvious replacement of generation and dominant with heterogenetic sporinite.
The sporogenesis of pteridophyte is a kind of shortcut of fast breeding seedling, it have simple, breeding amount it is big, into
Originally many advantages such as cheap, simple, are the main modes of fern breeding.Common practice is uniformly to sow spore
In matrix, transplanted into after cardioid gametophyte after spore development.But, this way is generally suffered from the drawback that：（1）Spore
Consumption is more, and seedling is few；（2）Spore development is slow；（3）It is excessive that artificial broadcast often results in density, is unfavorable for prothallus development；（4）One
Denier bacterium or fungal infection, then will spread rapidly, be easily caused original foliage death, terminate its development seedling.
Artificial seed is by the somatic embryo produced in Vitro Plant culture or the tissue that can develop into whole plant
It is included in the inside the shell containing nutriment and with defencive function, and the granule of seedling can be developed under optimum conditions,
Apply in the tissue cultures of multiple kinds of crops, industrial crops and rareness species, and breed with regard to pteridophyte artificial seed
Technology have no report.
Therefore it provides a kind of simple, survival rate is high, develop fast sporogenesis method, it is that pteridophyte production is anxious
The technical barrier that need to be solved.
The content of the invention
It is an object of the invention to provide it is a kind of without the need for transplanting, spore consumption is few, planting percent is high pteridophyta spore it is numerous soon
Method.
To reach above-mentioned purpose, the present invention is employed the following technical solutions：
A kind of pteridophyta spore quick-breeding method, comprises the following steps：
S1, collection pteridophyte maturation spore, go the removal of impurity；
S2, a great number of elements of MS, gibberellin, carbendazim and sodium alginate are made into coagulant liquid, then are stirred with spore, made
Spore coagulant liquid；
S3, absorption spore coagulant liquid, instill CaCl2Curing reaction is carried out in solution, spore ball is formed, then taken out, cleaned；
S4, with peat soil and perlite as matrix, sowing the previous day irrigated with carbendazim, one pallet water conservation of bottom pad；
S5, the stromal surface that spore ball is seeded in hole tray, per one, cave, without the need for earthing, cover moisturizing cover；
S6, maintenance management.
Further, the coagulant liquid is consisted of：NH4NO31650mg/L, KNO3 1900mg/L, KH2PO4 170mg/
L, MgSO4·7H2O 370mg/L, CaCl2·2H2O 440mg/L, 50 ~ 100mg/L of gibberellin, 0.1 ~ 0.2wt% of carbendazim, sea
Mosanom 1.5wt%.
Further, the ratio of the coagulant liquid and spore is every 100mL coagulant liquids addition 10mg spores.
Further, the CaCl2The concentration of solution is 1 ~ 3wt%, and the reaction time is 15 ~ 20min.
Further, the spore ball per contains 20 ~ 40 spores.
Further, the peat soil and perlitic volume ratio are 1：1.
Further, the maintenance management is：Environment temperature is 25 ~ 28 DEG C, and humid control is 80 ~ 90%；In 30d or so
Gametophyte clump is developed into, moisturizing cover is opened, ground moistening is kept, while being sprayed 1 ~ 2 time using 0.1% carbendazim solution daily.
The invention has the advantages that：
1st, the inventive method is adapted to annual sowing, without season limit.
2nd, growing-seedling period is shortened, planting percent is high.Gibberellin and nutrient are wrapped in artificial seed, effectively extend it
The rush effect of sprouting to spore, and maintain spore from sprouting to ripe gametophytic nutritional need is developed into, spore germination rate can be carried
High by 20 ~ 30%, spore artificial seed is formed from sowing to juvenile sporophyte clump and only needs 60d.
3rd, spore consumption is few.Fern seed artificial seed diameter is about 4mm, and 250 are can be made into per 10mg spores manually
Seed, develops into individual plant seedling to calculate with every artificial seed, and 10mg spores just can cultivate 250 plants of fern seedlings, greatly reduce spore
Sub- consumption, improves productivity effect.
4th, without the need for transplanting, seedling is once cultivated.Artificial seed is produced on the density that the nursery initial stage controls spore, solution
Traditional fern seed breeding of having determined is difficult to control to the problem of density.
5th, the present invention is the innovation to existing artificial seed.Existing artificial seed is mainly used in tissue training
Support in, aseptically cultivated, and fern artificial seed be under field conditions (factors), that is, have in collarium border train
Support, sugar is removed in embedding substance and fern artificial seed is developed under field conditions (factors) by bacteriostatic agent, additionally, manually
It is have its special significance that seed is used for fern seed breeding, you can accurately controls the spore quantity in artificial seed, reaches
The problem of control fern population densities.
In sum, tiny spore is made artificial seed by the present invention, is accelerated by wrapping up nutriment and bacteriostatic agent
The development of spore so as to develop into ripe gametophyte in a short time, while effective control spore density, without the need for transplanting, directly trains
It is bred as seedling.
Specific embodiment
With reference to specific embodiment, the present invention is described further：
Embodiment 1
According to the fast numerous pteridophyte of following steps：
S1, collection pteridophyte maturation spore, go the removal of impurity, it is impossible to 4 DEG C of refrigerations need to be placed in during sowing in time；
S2, fern artificial seed make：The a great number of elements of MS, gibberellin, carbendazim and sodium alginate are made into in proportion gel
Liquid：NH4NO31650mg/L, KNO3 1900mg/L, KH2PO4170mg/L, MgSO4·7H2O 370mg/L, CaCl2·2H2O
440mg/L, gibberellin 60mg/L, carbendazim 0.1wt%, sodium alginate 1.5wt%, pour 10mg spores into 100mL coagulant liquids, and
Stir, make spore coagulant liquid；
S3, it is that the dropper of 4mm draws spore coagulant liquid with bore, instills 2wt%CaCl2Curing reaction, 15min are carried out in solution
After form spore ball, then take out, in distilled water clean 1 ~ 2 time；The spore ball per of formation contains 20 ~ 40 spores, solidification
Calcium alginate Artificial Seed Coats are formed after reaction, by artificial endosperm（MS a great number of elements and gibberellin）Orbicule is rolled into spore,
The quick division growth needs of spore germination cell can be met；
S4, sowing media prepare：From peat soil and perlite with 1：1 volume ratio mixes, and sows the previous day with many bacterium of 0.1wt%
Spirit is irrigated, one pallet water conservation of bottom pad；
S5, the stromal surface that spore ball is seeded in hole tray, per one, cave, without the need for earthing, cover moisturizing cover；
S6, maintenance management：Environment temperature is 25 ~ 28 DEG C, and humid control is 80 ~ 90%；Spore ball culture 12d germination rates can reach
Gametophyte clump is developed into when 100%, 30d, moisturizing cover can be now opened, ground moistening is kept, while adopting bacterium more than 0.1% daily
Clever solution is sprayed 1 ~ 2 time, makes gametophyte clump have water membrane, and the amphigamy for not only improving spore egg cell and sperm has again
There is fungistatic effect, gametophyte Cong Jun initially forms juvenile sporophyte during 60d, planting percent obtains 250 plants of fern seedlings up to 100%.
Embodiment 2
According to the fast numerous pteridophyte of following steps：
S1, collection pteridophyte maturation spore, go the removal of impurity；
S2, fern artificial seed make：The a great number of elements of MS, gibberellin, carbendazim and sodium alginate are made into in proportion gel
Liquid：NH4NO31650mg/L, KNO3 1900mg/L, KH2PO4170mg/L, MgSO4·7H2O 370mg/L, CaCl2·2H2O
440mg/L, gibberellin 90mg/L, carbendazim 0.2wt%, sodium alginate 1.5wt%, pour 20mg spores into 200mL coagulant liquids, and
Stir, make spore coagulant liquid；
S3, it is that the dropper of 4mm draws spore coagulant liquid with bore, instills 3wt%CaCl2Curing reaction, 20min are carried out in solution
After form spore ball, then take out, in distilled water clean 1 ~ 2 time；The spore ball per of formation contains 20 ~ 40 spores, solidification
Calcium alginate Artificial Seed Coats are formed after reaction, by artificial endosperm（MS a great number of elements and gibberellin）Orbicule is rolled into spore,
The quick division growth needs of spore germination cell can be met；
S4, sowing media prepare：From peat soil and perlite with 1：1 volume ratio mixes, and sows the previous day with many bacterium of 0.1wt%
Spirit is irrigated, one pallet water conservation of bottom pad；
S5, the stromal surface that spore ball is seeded in hole tray, per one, cave, without the need for earthing, cover moisturizing cover；
S6, maintenance management：Environment temperature is 25 ~ 28 DEG C, and humid control is 80 ~ 90%；Spore ball culture 13d germination rates can reach
Gametophyte clump is developed into when 100%, 32d, moisturizing cover can be now opened, ground moistening is kept, while adopting bacterium more than 0.1% daily
Clever solution is sprayed 1 ~ 2 time, makes gametophyte clump have water membrane, and the amphigamy for not only improving spore egg cell and sperm has again
There is fungistatic effect, gametophyte Cong Jun initially forms juvenile sporophyte during 62d, planting percent obtains 487 plants of fern seedlings up to 100%.
The above, the only specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, any
Belong to those skilled in the art the invention discloses technical scope in, the change or replacement that can be readily occurred in, all should
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be defined by scope of the claims.
Claims (7)
1. a kind of pteridophyta spore quick-breeding method, it is characterised in that comprise the following steps：
S1, collection pteridophyte maturation spore, go the removal of impurity；
S2, a great number of elements of MS, gibberellin, carbendazim and sodium alginate are made into coagulant liquid, then are stirred with spore, made
Spore coagulant liquid；
S3, absorption spore coagulant liquid, instill CaCl2Curing reaction is carried out in solution, spore ball is formed, then taken out, cleaned；
S4, with peat soil and perlite as matrix, sowing the previous day irrigated with carbendazim, one pallet water conservation of bottom pad；
S5, the stromal surface that spore ball is seeded in hole tray, per one, cave, without the need for earthing, cover moisturizing cover；
S6, maintenance management.
2. pteridophyta spore quick-breeding method according to claim 1, it is characterised in that the coagulant liquid is consisted of：
NH4NO31650mg/L, KNO3 1900mg/L, KH2PO4170mg/L, MgSO4·7H2O 370mg/L, CaCl2·2H2O
440mg/L, 50 ~ 100mg/L of gibberellin, 0.1 ~ 0.2wt% of carbendazim, sodium alginate 1.5wt%.
3. pteridophyta spore quick-breeding method according to claim 1, it is characterised in that the ratio of the coagulant liquid and spore
Example is every 100mL coagulant liquids addition 10mg spores.
4. pteridophyta spore quick-breeding method according to claim 1, it is characterised in that the CaCl2The concentration of solution is
1 ~ 3wt%, the reaction time is 15 ~ 20min.
5. pteridophyta spore quick-breeding method according to claim 1, it is characterised in that the spore ball per containing 20 ~
40 spores.
6. pteridophyta spore quick-breeding method according to claim 1, it is characterised in that the peat soil and perlitic
Volume ratio is 1：1.
7. pteridophyta spore quick-breeding method according to claim 1, it is characterised in that the maintenance management is：Environment
Temperature is 25 ~ 28 DEG C, and humid control is 80 ~ 90%；Gametophyte clump is developed in 30d or so, moisturizing cover is opened, keeps soil wet
Profit, while being sprayed 1 ~ 2 time using 0.1% carbendazim solution daily.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610869204.8A CN106613814A (en) | 2016-09-30 | 2016-09-30 | Rapid propagation method of pteridophyte spores |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610869204.8A CN106613814A (en) | 2016-09-30 | 2016-09-30 | Rapid propagation method of pteridophyte spores |
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| CN106613814A true CN106613814A (en) | 2017-05-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610869204.8A Pending CN106613814A (en) | 2016-09-30 | 2016-09-30 | Rapid propagation method of pteridophyte spores |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110226385A (en) * | 2019-06-11 | 2019-09-13 | 深圳市铁汉生态环境股份有限公司 | A kind of type of seeding of pteridophyte |
| CN116584385A (en) * | 2022-09-01 | 2023-08-15 | 毕节市中药研究所 | Culture medium and method for inducing germination of pteris animalis spores and effectively culturing protophylls |
| CN118203021A (en) * | 2024-05-15 | 2024-06-18 | 中国医学科学院药用植物研究所 | Pteridium spore pill and preparation method thereof |
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| CN103651095A (en) * | 2013-12-26 | 2014-03-26 | 安徽理工大学 | Large-scale seaweed seedling culturing and transplanting method |
| CN104663200A (en) * | 2015-02-03 | 2015-06-03 | 深圳市铁汉生态环境股份有限公司 | Mass pteridophyta reproduction method |
| CN104754932A (en) * | 2012-06-01 | 2015-07-01 | 维多利亚农业服务控股公司 | Method for large scale generation of artificial seeds comprising symbiota |
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2016
- 2016-09-30 CN CN201610869204.8A patent/CN106613814A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104754932A (en) * | 2012-06-01 | 2015-07-01 | 维多利亚农业服务控股公司 | Method for large scale generation of artificial seeds comprising symbiota |
| CN103651095A (en) * | 2013-12-26 | 2014-03-26 | 安徽理工大学 | Large-scale seaweed seedling culturing and transplanting method |
| CN104663200A (en) * | 2015-02-03 | 2015-06-03 | 深圳市铁汉生态环境股份有限公司 | Mass pteridophyta reproduction method |
Non-Patent Citations (1)
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| 迟玉杰: "《食品添加剂》", 30 April 2013, 中国轻工业出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110226385A (en) * | 2019-06-11 | 2019-09-13 | 深圳市铁汉生态环境股份有限公司 | A kind of type of seeding of pteridophyte |
| CN116584385A (en) * | 2022-09-01 | 2023-08-15 | 毕节市中药研究所 | Culture medium and method for inducing germination of pteris animalis spores and effectively culturing protophylls |
| CN118203021A (en) * | 2024-05-15 | 2024-06-18 | 中国医学科学院药用植物研究所 | Pteridium spore pill and preparation method thereof |
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Application publication date: 20170510 |