CN106562894A - Skin-whitening, anti-aging and anti-allergic hesperidin composition, application and preparation - Google Patents
Skin-whitening, anti-aging and anti-allergic hesperidin composition, application and preparation Download PDFInfo
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- CN106562894A CN106562894A CN201610892623.3A CN201610892623A CN106562894A CN 106562894 A CN106562894 A CN 106562894A CN 201610892623 A CN201610892623 A CN 201610892623A CN 106562894 A CN106562894 A CN 106562894A
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- fectolinarin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/23—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/86—Polyethers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
Abstract
The invention belongs to the field of cosmetics and particularly relates to a skin-whitening, anti-aging and anti-allergic hesperidin composition, an application and a preparation. According to the composition containing hesperidin and pectolinarin, provided by the invention, the number of melanin particle-containing positive cells and the content of tyrosinase can be remarkably reduced, the SOD activity can be remarkably improved, the MDA content can be reduced, and the ear swelling and the licking frequency of an experimental animal can be remarkably reduced; and the hesperidin and the pectolinarin are compounded to achieve the effects of remarkably whitening skin, resisting aging and resisting allergy.
Description
Technical field
The invention belongs to cosmetic field, and in particular to whitening and anti-aging, antianaphylactic Pericarpium Citri junoriss glycoside composition and purposes and
Preparation.
Background technology
In recent years, with the aggravation of people's life, the quickening of work rhythm and environmental pollution, the skin that people face is asked
Topic is more and more, and especially obscure, pigmentation of the colour of skin etc. becomes common problem, whitening, anti aging effect cosmetics also because
This becomes a focus of skin care item development field.Increasingly mature with people's consumption idea, consumer is to such cosmetics
Focal point be not only only that its is both effectiveness, while also more focusing on its safety.Compare with such as zest security risk
Chemical whitening, antioxidant for, the natural whiting in Chinese medicine source, antidotal agent have safety, gentle, persistently, efficiently etc.
Advantage a, it has also become main trend of skin-lightening cosmetic research and development now.
As Chinese patent application 201110285018.7 discloses a kind of Chinese herbal medicine skin care whitening anti-aging wrinkle removing despeckle
Cosmetic compositions, it includes cosmetics emulsifying base and Chinese medicine extract, and the Chinese medicine extract is Stigma Croci, Ganoderma, white
Art, the Radix Paeoniae Alba, Poria, Radix Ampelopsis, Radix Glycyrrhizae, Cortex Fraxini, the Pseudobulbus Bletillae (Rhizoma Bletillae), Folium Pini, Folium Menthae extract, it not only can improve skin metabolism, promote
Enter cutaneous circulation, increase skin-nourishing, and can remove wrinkle and mottle that skin occurs, make skin fine and smooth, smooth, tender
White and springiness, is cosmetic composition product that is a kind of novel and showing unique characteristics.But said composition whitening Chinese medicament composition is numerous
It is many, complicated process of preparation, simultaneously because there are substantial amounts of oil-soluble Chinese medicinal components in said composition, it is under high and low temperature environment
Easily separate out, need the stability for adding more co-emulsifiers to keep compositionss.And more emulsion adjuvant is deposited
Zest of the compositionss to skin is then being increased, consumer easily causes skin allergy, and the combination using the based composition
Thing whitening and anti-aging effect is not notable.
Therefore, it is necessary to providing, a kind of composition is simple, stability is high, and its can whitening effect, defying age, antianaphylactic group
Compound.
Hesperidin, is flavanone glycoside compound, in being distributed widely in many plants, acts to have maintaining osmotic pressure, is increased
Strong blood capillary toughness, shortens the bleeding time, reduces the effect such as cholesterol, is usually used in the auxiliary treatment of cardiovascular system diseases.
Pectolinarin, also known as Fectolinarin, from the dry aerial parts or root of feverfew Ji, molecular formula C28H34O15；Molecule
Amount 622.56；Structural formula is as follows：
At present, Hesperidin and Fectolinarin are combined for preparing the cosmetics with whitening, antioxidation and antianaphylaxis function
Compositionss have no and have been reported that.
The content of the invention
In order to solve technical problem present in prior art, it is an object of the invention to provide a kind of have whitening, resist
Aging, the Pericarpium Citri junoriss glycoside composition of antianaphylaxis function and purposes and preparation, to solve disadvantages described above.
The invention provides a kind of Pericarpium Citri junoriss glycoside composition with whitening and anti-aging, antianaphylaxis function, including Hesperidin and
Fectolinarin, the weight ratio of Hesperidin and Fectolinarin is 1 in the compositionss:(0.6～1).
Another object of the present invention is to provide above-mentioned compositionss containing Hesperidin and Fectolinarin to have whitening, anti-ageing preparing
Always, the purposes in the cosmetics of antianaphylaxis function.
Preferably, content of the compositionss containing Hesperidin and Fectolinarin in cosmetics is 0.1～8%.
Preferably, content of the compositionss containing Hesperidin and Fectolinarin in cosmetics is 5%.
Preferably, the weight ratio of Hesperidin and Fectolinarin is 1 in the compositionss containing Hesperidin and Fectolinarin:(0.6～
1)。
Preferably, the weight ratio of Hesperidin and Fectolinarin is 1 in the compositionss containing Hesperidin and Fectolinarin:0.8.
Preferably, the cosmetics are made into unguentum, Emulsion, membrane, solution, gel or soap agent.
Wherein, the cosmetics include facial cream, emulsion, essence, facial milk cleanser, facial film, sunscreen cream, BB frosts etc..
Present invention also offers a kind of Hesperidin unguentum with whitening and anti-aging, antianaphylaxis function, including following weight
The preparing raw material of number：It is compositionss 0.1～8% containing Hesperidin and Fectolinarin, olive oil 1～5%, emulsifying agent 2～10%, sweet
Oil 10～25%, silicone oil 5～12%, cetearyl alcohol 3～6% and containing inorganic salt solution 40～60%, the emulsifying agent by
Hydrolecithin and Macrogol 4000 press 1：The weight of (0.02～0.06) is than composition.
Preferably, the weight ratio of Hesperidin and Fectolinarin is 1 in the compositionss containing Hesperidin and Fectolinarin:(0.6～
1)。
Preferably, the inorganic salt is calcium sulfate, and calcium sulfate content in aqueous is 0.1～5%.
Correspondingly, present invention also offers a kind of method for preparing above-mentioned unguentum, comprises the following steps：
A the compositionss containing Hesperidin and Fectolinarin, emulsifying agent, silicone oil and cetearyl alcohol) are taken to be dissolved in olive oil, is mixed
It is even, obtain oil phase；
B) take glycerol to be dissolved in containing inorganic salt solution, mix, obtain water phase；
C) oil phase and water are mutually stirred at room temperature after mix homogeneously and are warming up to 30～50 DEG C of 1～5min of melting, homogenizing 10
～30min, obtains final product.
Preferably, step C) in be warming up to 38 DEG C, melt 3min, homogenizing 20min.
Inventor has found before this Hesperidin and Radix Cirsii Japonici glycoside composition has obvious therapeutical effect to metabolism syndrome, and
Application, Application No. are proposed to State Intellectual Property Office：201610634618.2.And make inventor surprisingly,
In process of the test, inventor's accident ground finds Hesperidin and Radix Cirsii Japonici glycoside composition except effectively improving metabolism syndrome
Therapeutical effect, it can also substantially reduce the content of positive cell number containing melanin granule and tryrosinase, and can significantly improve
Mouse aging skin SOD activity and reduction MDA contents, this illustrates that it has significant whitening, oxidation-resisting and caducity effect.And enter
One step ground, Jing mice ear experimental result finds that Hesperidin has with the compositionss that Fectolinarin is constituted in the range of certain proportion
Play the role of to significantly reduce ear swelling degree and laboratory animal licks body number of times, and both compound the effects with Synergistic.
On the basis of the above, the invention provides it is a kind of containing electrolyte aqueous solution/hydrolecithin-Macrogol 4000/
Fructus Canarii albi oil systems, by the temperature for changing system, have been prepared high performance containing Hesperidin and big using the phase in version of system
The oil-in-water unguentum of Ji glycosides.Wherein, the emulsifying agent of both hydrolecithin-Macrogol 4000s compounding composition, can substantially carry
The stability of high unguentum.
The temperature for needing offer higher when oil-in-water unguentum is prepared can just be allowed to phase in version.Inventor's discovery, portion
The presence of point electrolyte can to a certain extent reduce the phase transition temperature of system.Therefore, inventor attempts adding various electrolysis
Matter, such as Sodium Chloride, calcium chloride, sodium sulfate and calcium sulfate, it is found that calcium sulfate can substantially reduce the phase transition temperature of system, and
The addition of Sodium Chloride can not reduce the phase transition temperature of system of the present invention.
Compared with prior art, the present invention has the advantage that：
1) compositionss containing Hesperidin and Fectolinarin that the present invention is provided can substantially reduce positive thin containing melanin granule
The content of born of the same parents' number and tryrosinase, can significantly improve SOD activity and reduce MDA contents, and can significantly reduce ear swelling degree and
The effect for licking body number of times of laboratory animal, both compound has significant whitening and anti-aging and antianaphylactic effect.
2) present invention also offers a kind of unguentum of oil-in-water containing Hesperidin, it has stability high, low production cost, energy consumption
Few advantage.
Specific embodiment：
Below by way of the description of specific embodiment, the invention will be further described, but this is not the limit to the present invention
System, those skilled in the art's basic thought of the invention, various modifications may be made or improves, but without departing from this
The basic thought of invention, within the scope of the present invention.
The preparation of embodiment 1, unguentum
Take compositionss 5kg containing Hesperidin and Fectolinarin, emulsifying agent 10kg, silicone oil 8kg and cetearyl alcohol 4kg and be dissolved in olive
In olive oil 3kg, mix, obtain oil phase；Take glycerol 10kg and be dissolved in sulfur acid calcium aqueous solution 40kg, mix, obtain water phase；By oil phase and water
Mutually it is stirred at room temperature after mix homogeneously and is warming up to 38 DEG C of meltings 3min, homogenizing 20min, obtains final product, wherein, the emulsifying agent is by hydrogen
Change lecithin and Macrogol 4000 presses 1：0.03 weight ratio composition, wherein, the compositionss containing Hesperidin and Fectolinarin
The weight ratio of middle Hesperidin and Fectolinarin is 1:0.8.
The preparation of embodiment 2, Emulsion
Take compositionss 8kg containing Hesperidin and Fectolinarin, emulsifying agent 8kg to be dissolved in olive oil 3kg, mix, obtain oil phase；Take
Glycerol 22kg is dissolved in sulfur acid calcium aqueous solution 50kg, mixes, and obtains water phase；Oil phase is mutually stirred at room temperature after mix homogeneously with water
38 DEG C of meltings 3min, homogenizing 20min are warming up to, are obtained final product, wherein, the emulsifying agent is by dodecyl sodium sulfate and Polyethylene Glycol
4000 press 1：0.02 weight than composition, wherein, Hesperidin and Fectolinarin in the compositionss containing Hesperidin and Fectolinarin
Weight ratio is 1:1.
The preparation of embodiment 3, membrane
Compositionss 4kg containing Hesperidin and Fectolinarin and the heating of 8kg polysorbate80s are allowed to dissolve, 0.3kg is added
Carbomer, adds 0.3kg triethanolamine to adjust pH to 7.0 after stirring evenly, add 65kg deionized waters, 10kg polyvinyl alcohol, heating
It is allowed to dissolve, obtains final product, wherein, the weight ratio of Hesperidin and Fectolinarin is 1 in the compositionss containing Hesperidin and Fectolinarin:
0.6。
The preparation of embodiment 4, gel
Take 6kg glycerol, 1kg carbomers to be dissolved in 68kg deionized waters, stir, add containing Hesperidin and Fectolinarin
Compositionss 2.5kg, 8kg polysorbate80 and ethanol 2kg, stirring is allowed to dissolve, and obtains final product, wherein, it is described containing Hesperidin and big
The weight ratio of Hesperidin and Fectolinarin is 1 in the compositionss of Ji glycosides:1.
Test example one, whitening is tested
1.1 laboratory animal
SPF levels male yellowish-brown Cavia porcelluss, weight 250-270g, credit number：SCXK (Guangdong) 2008-0002, by Guangzhou
University of TCM's Experimental Animal Center is provided.
1.2 experimental technique
Using yellowish-brown Cavia porcelluss 60, it is divided into 6 groups, respectively matched group, Hesperidin low dose group, Hesperidin high dose
Group, Fectolinarin group, test group and contrast groups, 10 per group.Each group percutaneous drug delivery concentration is as follows：Matched group：Dermal application equivalent
Normal saline；Hesperidin low dose group：The Hesperidin of 1.8g/kg；Hesperidin high dose group：The Hesperidin of 3.6g/kg；Radix Cirsii Japonici
Glycosides group：The Fectolinarin of 3.6g/kg；Test group：The Fectolinarin of the Hesperidin+2.88g/kg of 3.6g/kg；Contrast groups：1.2g/kg
The Fectolinarin of Hesperidin+3.6/kg.Administration group goes to hair-fields in back both sides shaving into some 1cm × 2cm sizes, is existed with swab stick
Left and right side skin will be administered and apply a circle.2 times a day.Blank group left and right side depilation skin is set to blank skin.
After 28 days, take Cavia porcelluss skin and take skin biopsies, use 10% formalin fix, paraffin embedding, section are seen
Examine.Histological observation:Cma staining, the Histological change of every part of specimen of light Microscopic observation, to containing melanin in basal cell
Granular cell is counted.The average of per group of 10 Cavia porcelluss is drawn, and carries out comparing between group.Will according to the quantity containing melanin granule cell
The dermal melanin content of test group of animals is divided into 4 grades (+～++++), and difference is as follows：
+：Less than 20 cell containing melanin granule/100 basal cells；++：The individual cells containing melanin granule of 21-30/
100 basal cells；+++：31-40 cell containing melanin granule/100 basal cell；++++：Contain melanin more than 40
Granulocyte/100 basal cell.
Cavia porcelluss blood is prepared, 3000r/min centrifugation 20min take supernatant, and Elisa methods determine each group animal serum L-Tyrosine
The content of enzyme.
1.3 statistical method
Statistical procedures are carried out with the softwares of SPSS17 0, is represented with average ± standard deviationRepresent, using Dan Yin
Plain variance analyses, P<0.05 is that difference is statistically significant.
1.4 experimental result
Table 1 is to impact of the guinea pig skin containing melanin granule positive cell number(n=10)
Compare with matched group：*p<0.05, * * p<0.01；Compared with contrast groups,P ＜ 0.05.
Impact of the table 2 to guinea pig serum tryrosinase content (n=10,))
| Group | Tryrosinase content (pg/ml) |
| Matched group | 281.6±46.3 |
| Hesperidin low dose group | 236.5±32.8* |
| Hesperidin high dose group | 212.4±28.1* |
| Fectolinarin group | 268..5±38.8 |
| Contrast groups | 210.6±26.7** |
| Test group | 150.2±11.4** |
Compared with matched group：* P ＜ 0.05, * * P ＜ 0.01；Compared with contrast groups,P ＜ 0.05.
From Tables 1 and 2, compared with matched group, Hesperidin low dose group, Hesperidin high dose group, contrast groups, test
Group animal positive cell number containing melanin granule and skin tryrosinase content are remarkably decreased；Compared with contrast groups, test group is moved
Thing positive cell number containing melanin granule and skin tryrosinase content pole substantially reduce.Illustrate the present invention offer Hesperidin with
Mutually coordinated of the compositionss that Fectolinarin is constituted in the range of certain proportion reduce positive cell number containing melanin granule and reduction
The effect of tryrosinase content, with significant whitening effect.
Test example two, anti-skin oxidative aging experiment
2.1 laboratory animal
SPF level NIH mices, weight 18-22g, male and female half and half, credit number：SCXK (Guangdong) 2008-0002, by Guangzhou
University of TCM's Experimental Animal Center is provided.
2.2 experiment material
Superoxide dismutase (SOD), total antioxidant capacity (T-AOC) determine reagent, and box, Nanjing is built up biological engineering and ground
Study carefully institute；D- galactose biochemical reagents, Chemical Reagent Co., Ltd., Sinopharm Group.
2.3 experimental technique
It is Kun Ming mice 70, respectively matched group, model group, Hesperidin low dose group, Hesperidin high dose group, big
Ji glycosides group, test group and contrast groups, 10 per group.The daily nape part injecting normal saline 0.3mL of matched group, remaining 6 groups are given
1g/kg galactose 0.3mL nape part subcutaneous injections, successive administration 28d is given to set up subacute aging model, while skin of back is applied
Medicine, each group percutaneous drug delivery dosage is as follows：Matched group：Smear the normal saline of equivalent；Model group：Smear the normal saline of equivalent；
Hesperidin low dose group：The Hesperidin of 1.8g/kg；Hesperidin high dose group：The Hesperidin of 3.6g/kg；Fectolinarin group：3.6g/
The Fectolinarin of kg；Test group：The Fectolinarin of the Hesperidin+2.88g/kg of 3.6g/kg；Contrast groups：The Hesperidin of 1.2g/kg+
The Fectolinarin of 3.6/kg.Following index in the homogenate of mouse back skin histology is determined after 6 weeks：Superoxide dismutase (SOD) is living
Property, oxidisability index malonaldehyde (MDA) content.
The detection of 2.4SOD and MDA enzymes
After test last dose 1h, cervical dislocation puts to death mice, and skin of unhairing is each one piece to take back.Use pre-cooling physiology
The impurity such as (4 degree) flushings of saline, cleared blood, filter paper wipes dry, cuts skin, liver respectively about 0.5g, and operating scissorss are shredded, with tissue
Dismembyator is homogenized, with 1:9 (W/V) add ice normal saline, make 10% tissue homogenate.Homogenate is with 3500rmin-1Centrifugation
15min, takes the OD values that supernatant determines SOD, MDA on ultraviolet spectrophotometer, calculates its SOD activity and MDA contents.
Impact of the table 3 to SOD, MDA of mouse aging skin
| Group | SOD activity (U/mg) | MDA contents (nmol/mg) |
| Matched group | 114.6±20.3 | 3.02±0.85 |
| Model group | 68.2±9.6** | 4.68±1.19** |
| Hesperidin low dose group | 92.3±11.4△△ | 4.01±0.93△ |
| Hesperidin high dose group | 98.7±13.5△△ | 3.96±0.96△ |
| Fectolinarin group | 71.6±15.2 | 4.13±1.18 |
| Test group | 110.5±16.7△△ | 3.08±0.72△△ |
| Contrast groups | 97..2±10.1△△ | 3.92±1.11△ |
Compared with matched group：* P ＜ 0.01；Compared with model group：△P ＜ 0.05,△△P ＜ 0.01；Compared with contrast groups,
P ＜ 0.05.
As shown in Table 3, compared with model group, in addition to Fectolinarin group, administration each group SOD activity pole is significantly higher than model group,
MDA contents are substantially less than model group；Compared with contrast groups, it is active that test group can significantly improve SOD, reduces MDA contents.Say
Mutually coordinated of the compositionss that the Hesperidin that the bright present invention is provided is constituted with Fectolinarin in the range of certain proportion improve SOD and live
Property, the effect of MDA contents is reduced, especially when Hesperidin and Fectolinarin press 1:1 ratio combination has significant defying age oxygen
Change is acted on.
Test example three, antiallergic research
3.1 mice ear are tested
3.1.1 laboratory animal
SPF level NIH mices, weight 18-22g, male and female half and half, credit number：SCXK (Guangdong) 2008-0002, by Guangzhou
University of TCM's Experimental Animal Center is provided.
3.1.2 experiment packet and method
60 mices are respectively into model group, Hesperidin low dose group, Hesperidin high dose group, Fectolinarin group, test group
And contrast groups, 10 per group, each group percutaneous drug delivery concentration is following such as test example two.Respectively two sides applies each group mice before and after auris dextra
Caused by dimethylbenzene xylene scorching (0.02mL/ is only), causes to embrocate reagent after 1h with the said medicine of same dose respectively in auris dextra after inflammation,
Model group applies normal saline.Mice cervical dislocation is put to death after 4h, cuts ears, it is identical in left and right ear with 8mm card punch
Round auricle is laid at position, is weighed in analytical balance, with two auricle weight differences as inflammation swelling, and calculates inhibitory rate of intumesce.
Average swelling * 100% of the average swelling/matched group of the average swelling-administration group of suppression ratio (%)=matched group
3.1.3 experimental result
Impact of the table 4 to ear swelling degree
| Group | Swelling (mg) | Inhibitory rate of intumesce (%) |
| Model group | 2.28±0.27 | - |
| Hesperidin low dose group | 1.76±0.25△△ | 22.8 |
| Hesperidin high dose group | 1.68±0.21△△ | 26.3 |
| Fectolinarin group | 2.04±0.29 | 10.5 |
| Test group | 1.18±0.20△△ | 48.2 |
| Contrast groups | 1.62±0.24△△ | 29.2 |
Compared with model group：△△P ＜ 0.01；Compared with contrast groups,P ＜ 0.01.
As shown in Table 4, compared with model group, in addition to Fectolinarin group, administration each group ear swelling degree is substantially less than model group；With
Contrast groups are compared, and test group extremely can significantly reduce ear swelling degree.Illustrate the Hesperidin and Fectolinarin of present invention offer one
The compositionss of composition are mutually coordinated in the range of certainty ratio plays a part of to reduce ear swelling degree, especially when Hesperidin is pressed with Fectolinarin
1:There are extremely significant anti-allergic effects when 1 ratio is combined.
3.2 antipruritic experiments
3.2.1 laboratory animal
SPF level NIH mices, weight 18-22g, male and female half and half, credit number：SCXK (Guangdong) 2008-0002, by Guangzhou
University of TCM's Experimental Animal Center is provided.
3.2.2 experiment packet and method
70 mices are divided into into matched group, model group, Hesperidin low dose group, Hesperidin high dose group, Fectolinarin group, are tried
Group and contrast groups are tested, 10 per group, each group percutaneous drug delivery concentration is following such as test example two.It is de- at back with 8% sodium sulfide solution
Hair (2cm × 3cm), by reagent is applied on hair removal section skin, once a day, successive administration 7d, model group and matched group are smeared
Normal saline.Subcutaneous injection 4-aminopyridines of the last dose 40min after nape part coating position (match somebody with somebody by normal saline
Into 0.02%) solution 0.01mL/g, matched group injecting normal saline.Record each group licks body number of times (continuously to lick in 10min
Body licks 1 calculating of body to there is minibreak).
3.2.3 experimental result
Impact test (n=10) that 5 pairs, table licks body number of times
| Group | 10min licks body number of times |
| Matched group | 0 |
| Model group | 46.3±7.6 |
| Hesperidin low dose group | 29.5±6.8△△ |
| Hesperidin high dose group | 24.1±6.3△△ |
| Fectolinarin group | 43.8±8.6 |
| Test group | 22.1±6.1△△ |
| Contrast groups | 29.2±6.2△△ |
Compared with model group：△△P ＜ 0.01；Compared with contrast groups,P ＜ 0.05.
As shown in Table 5, compared with model group, in addition to Fectolinarin group, administration each group animal licks body number of times considerably less than model
Group；Compared with contrast groups, test group extremely can be reduced significantly licks body number of times.The Hesperidin and Fectolinarin of present invention offer are provided
The compositionss of composition are mutually coordinated in the range of certain proportion plays antianaphylactic effect, especially when Hesperidin and Fectolinarin press 1:
There is extremely significant antipruritic effect when 1 ratio is combined.
Test example four, stability test
Sample described in Example 1, and (emulsifying agent is hydrolecithin, remaining composition such as embodiment to arrange matched group 1
1), matched group 2 (emulsifying agent is Polyethylene Glycol, remaining composition such as embodiment 1), by 3 groups in 40～50 DEG C of electro-heating standing-temperature cultivators
It is middle to place 30～50 days, recover to be observed after room temperature, as a result show, sample is at 40～50 DEG C described in the embodiment of the present invention 1
After placing 30～50 days, without layering, precipitation phenomenon, with excellent stability.And matched group 1 and matched group 2 are occurred in that not
With the layering of degree, precipitation phenomenon.
Claims (10)
1. a kind of Pericarpium Citri junoriss glycoside composition with whitening and anti-aging, antianaphylaxis function, it is characterised in that including Hesperidin and big
Ji glycosides, the weight ratio of Hesperidin and Fectolinarin is 1 in the compositionss:(0.6～1).
2. use of the compositionss containing Hesperidin and Fectolinarin in preparing with whitening and anti-aging, the cosmetics of antianaphylaxis function
On the way.
3. purposes as claimed in claim 2, it is characterised in that the compositionss containing Hesperidin and Fectolinarin are in cosmetics
Content be 0.1～8%.
4. as claimed in claim 2 or claim 3 purposes, it is characterised in that Pericarpium Citri junoriss in the compositionss containing Hesperidin and Fectolinarin
The weight ratio of glycosides and Fectolinarin is 1:(0.6～1).
5. purposes as claimed in claim 4, it is characterised in that in the compositionss containing Hesperidin and Fectolinarin Hesperidin and
The weight ratio of Fectolinarin is 1:0.8.
6. purposes as claimed in claim 2, it is characterised in that the cosmetics be made into unguentum, Emulsion, membrane, solution,
Gel or soap agent.
7. a kind of Hesperidin unguentum with whitening and anti-aging, antianaphylaxis function, it is characterised in that including following parts by weight
Preparing raw material：Compositionss 0.1～8% containing Hesperidin and Fectolinarin, olive oil 1～5%, emulsifying agent 2～10%, glycerol 10
～25%, silicone oil 5～12%, cetearyl alcohol 3～6% and containing inorganic salt solution 40～60%, the emulsifying agent is by hydrogenating
Lecithin and Macrogol 4000 press 1：The weight of (0.02～0.06) is than composition.
8. unguentum as claimed in claim 7, it is characterised in that in the compositionss containing Hesperidin and Fectolinarin Hesperidin and
The weight ratio of Fectolinarin is 1:(0.6～1).
9. unguentum as claimed in claim 7, it is characterised in that the inorganic salt is calcium sulfate, and the calcium sulfate is in aqueous solution
In content be 0.1～5%.
10. a kind of method for preparing the unguentum as described in claim 7～9 is arbitrary, it is characterised in that comprise the following steps：
A the compositionss containing Hesperidin and Fectolinarin, emulsifying agent, silicone oil and cetearyl alcohol) are taken to be dissolved in olive oil, is mixed, obtained
Oil phase；
B) take glycerol to be dissolved in containing inorganic salt solution, mix, obtain water phase；
C) oil phase and water are mutually stirred at room temperature after mix homogeneously and are warming up to 30～50 DEG C of 1～5min of melting, homogenizing 10～
30min, obtains final product.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610892623.3A CN106562894B (en) | 2016-10-12 | 2016-10-12 | Whitening and anti-aging, antianaphylactic orange peel glycoside composition and purposes and preparation |
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| CN201610892623.3A CN106562894B (en) | 2016-10-12 | 2016-10-12 | Whitening and anti-aging, antianaphylactic orange peel glycoside composition and purposes and preparation |
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| CN106562894A true CN106562894A (en) | 2017-04-19 |
| CN106562894B CN106562894B (en) | 2019-06-11 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107737059A (en) * | 2017-12-04 | 2018-02-27 | 刘晓冰 | A kind of centella whitening lotion |
| CN108158884A (en) * | 2018-02-01 | 2018-06-15 | 百朗德生物化学(海门)有限公司 | With the cosmetic material composition and its application in cosmetics for inhibiting allergy, function of delaying senility |
| CN111110583A (en) * | 2020-01-19 | 2020-05-08 | 广东药科大学 | Composition for whitening skin, removing acne, removing blackheads and resisting aging as well as application and preparation thereof |
-
2016
- 2016-10-12 CN CN201610892623.3A patent/CN106562894B/en active Active
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107737059A (en) * | 2017-12-04 | 2018-02-27 | 刘晓冰 | A kind of centella whitening lotion |
| CN108158884A (en) * | 2018-02-01 | 2018-06-15 | 百朗德生物化学(海门)有限公司 | With the cosmetic material composition and its application in cosmetics for inhibiting allergy, function of delaying senility |
| CN111110583A (en) * | 2020-01-19 | 2020-05-08 | 广东药科大学 | Composition for whitening skin, removing acne, removing blackheads and resisting aging as well as application and preparation thereof |
| WO2021143096A1 (en) * | 2020-01-19 | 2021-07-22 | 广东药科大学 | Whitening, acne-removing, blackhead-removing and anti-aging composition, and use and preparation thereof |
| CN111110583B (en) * | 2020-01-19 | 2022-01-14 | 广东药科大学 | Composition for whitening skin, removing acne, removing blackheads and resisting aging as well as application and preparation thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106562894B (en) | 2019-06-11 |
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