CN106511151B

CN106511151B - A kind of chlorophyll is sun-proof and after-sun skin care item

Info

Publication number: CN106511151B
Application number: CN201610930673.6A
Authority: CN
Inventors: 陈松彬; 易萍; 倪彦艳; 刘杰森; 张爱萍; 李小翠
Original assignee: Guangdong Kewei Biological Technology Co Ltd
Current assignee: Guangdong Huasang Lixi Biotechnology Co ltd
Priority date: 2016-10-31
Filing date: 2016-10-31
Publication date: 2017-08-15
Anticipated expiration: 2036-10-31
Also published as: CN106511151A

Abstract

The invention belongs to cosmetic technical field, it is related to that a kind of chlorophyll is sun-proof and after-sun skin care item.Chlorophyll of the present invention is sun-proof and after-sun skin care item are by chlorophyll complex liposome, hydrogenated jojoba oil, amygdalus pedunculata pall oil, saualane, ring five dimethyl silicone polymer, cetostearyl alcohol, the stearates of PEG 100, tocopherol acetate, bisabolol, butanediol, beta glucan, Sodium Hyaluronate, carbomer, trehalose, cetearyl glucoside, VCE, moringa oleifera leaf extractive, triethanolamine, preservative, essence and water are prepared, property is stable, it is safe, comfort is good, the ultraviolet of different-waveband can be defendd effectively, also there is good anti-light aging function simultaneously, skin aging caused by Exposure to Sunlight can be prevented, spot, the symptoms such as decortication, promote the after-sun of skin.

Description

A kind of chlorophyll is sun-proof and after-sun skin care item

Technical field

The invention belongs to cosmetic technical field, it is related to that a kind of chlorophyll is sun-proof and after-sun skin care item.

Background technology

As people deepen continuously to ultraviolet protection importance and necessity understanding, the demand in sun care preparations market Also it is being continuously increased, safe efficient, wide spectrum sunscreen product is increasingly becoming focus of concern.Sun-screening agent is sun-proof cosmetic The key component of anti-sunlight function is played in product, sun-screening agent on the market is broadly divided into physical ultraviolet light screener and chemistry at present The ultraviolet absorber of property.Physical sunscreen agent is to no skin irritation, but absorbability, the transparency and poor air permeability, anti-for preparing Cosmetics are shone, product comfort has much room for improvement.Chemical sun has certain stimulation to skin, may cause sensitivity The allergy and stimulate the reaction of skin, meanwhile, part chemical sun light resistance and photostability be not high, easily by light degradation, is used for Sun care preparations is prepared, the safety and stability of product has much room for improvement.

Research shows that the natural plant composition such as rutin, scutelloside, barbaloin, aloe polysaccharide, LBP-X, Quercetin can Play a part of preventing ultraviolet injury, reparation skin, anti-aging by absorbing ultraviolet or removing free radical etc..Meanwhile, Chemical composition of Chinese materia medica property with anti-sunlight function is gentle, no skin irritation, and decapacitation is absorbed outside ultraviolet, and also having concurrently for having is controlled Treat solar dermatitis, the lustrous and transparent face of skin care and freckle removing and whitening effect.As chemical sun is continuous to side effect such as allergy of skin etc. Occur, and people go deep into its toxic side effect understanding, and the uvioresistant material of safe and nontoxic evil is found from natural products One of focus of sun care preparations research and development is become.The usage amount of chemical sun is reduced, improves sun care preparations Performance, improves the content of natural component in Product Safety, increase product, is always the effort side of the sun care preparations producers To.

The B of Chinese patent CN 103405363 disclose a kind of sun-proof repair combination, are extracted by aloe extract, radix scutellariae Thing, green-tea extract, green stone lotus extract, Nano titanium dioxide, vitamin E, SP, isomery hexadecane, jojoba oil, angle Shark alkane, anhydrous sorbitol laurate, sorbitan monooleate, glycerine, xanthans, essence, preservative and water prepare and Into, the ultraviolet of different-waveband can effectively be absorbed and Isolated Shield, and with the effect for repairing skin.

The B of Chinese patent CN 103340798 disclose a kind of natural plant sun cream, different pungent by water, glycerine, palmitic acid Ester, extract solution from aloe, radix scutellariae extract solution, red bayberry extract solution, fatty alcohol, bisabolol, dimethyl silicone polymer, the poly- second of cetyl Glycol/polypropylene glycol -10/1- dimethyl silscones, methyl hydroxybenzoate, Nipasol and daily essence is prepared from, and can effectively be obstructed With absorption ultraviolet, resistivity of the human skin cell to ultraviolet is improved, anti-treating disorders in muscles and skin is tanned, tan severely and aging, whitening Nti-freckle.

Chlorophyll belongs to porphyrins, is that most one of maximum natural pigment of wide, yield is distributed in nature, has Antibacterial, anti-inflammatory, antitumor and antimutagenesis.Therefore, chlorophyll and its derivative are obtained in food, medicine and filed of daily-use chemical industry To extensive use.

Now there are some researches show chlorophyll, which acts on skin, to be had antipollution, radioresistance, removes free radical, anti-light aging, prolongs Slow aging, protection skin environment, promotion wound healing, deodorization and other effects, have broad application prospects in cosmetic field.Mesh Preceding chlorophyll is mainly used as colouring agent and deodorant in cosmetic field.Chlorophyll is natural, safe, nontoxic without sensitivity, can Absorb simultaneously and scatters ultraviolet, safe for preparing sun care preparations with multiple defense ultraviolet effect, sun-proof effect Fruit is significantly.It is sensitive to factors such as light, acid, alkali and oxygen but chlorophyll stability is poor, easily decompose inactivation.

Therefore, chlorophyll is applied to during sun care preparations suitable system need to be selected to enable chlorophyll retention properties stable, The leaf that research and develop that a kind of property is stable, safe, comfort is good, can effectively defend the ultraviolet of different-waveband is green Plain sun-proof and after-sun skin care item have wide market prospects.

The content of the invention

It is an object of the invention to overcome existing technological deficiency, there is provided a kind of chlorophyll is sun-proof and after-sun skin care Product.Chlorophyll of the present invention is sun-proof and after-sun skin care item property is stable, and safe, comfort is good, to different-waveband Ultraviolet can effectively defend, while also have good anti-light aging function, can prevent skin aging caused by Exposure to Sunlight, The symptoms such as spot, decortication, promote the after-sun of skin.

Technical scheme is as follows：

A kind of chlorophyll is sun-proof and preparation methods of after-sun skin care item, it is characterised in that comprise the following steps：

1) hydrogenated jojoba oil 2-6%, amygdalus pedunculata pall oil 2-6%, saualane 2-5%, ring five by weight percentage, are taken Dimethyl silicone polymer 2-5%, cetostearyl alcohol 1-3%, PEG-100 stearate 1-2%, tocopherol acetate 0.5-1%, Bisabolol 0.1-0.3% and cetearyl glucoside 2-4% is added in oil cauldron, is heated to 80-85 DEG C, constant temperature stirring To being completely dissolved, material A is obtained；

2) by weight percentage, take butanediol 3-10%, Sodium Hyaluronate 0.05-0.2%, carbomer 0.1-0.4%, Trehalose 0.5-3%, VCE 0.2-0.8% and water 38-75% are added in aqueous phase pot, are heated to 80-85 DEG C, constant temperature, which is stirred to after being completely dissolved, to be pumped into warmed-up emulsifying pot, opens stirring, material A is slowly pumped into emulsification In pot, emulsifying is carried out, speed of agitator is 2500-3000r/min, and the emulsifying time is 5-10min, and vacuum defoamation is opened Begin to cool；

3) when being cooled to 40-45 DEG C, by weight percentage, chlorophyll complex liposome 5-10%, beta glucan are added 0.2-0.5%, preservative 0.6-1.5%, moringa oleifera leaf extractive 1-4%, triethanolamine 0.1-0.4% and essence 0.01- 0.1%, second of emulsifying is carried out, speed of agitator is 500-700r/min, and the emulsifying time is 2-5min, and vacuum takes off Bubble, is down to room temperature, sampling Detection, qualified discharging is produced；

Wherein, the chlorophyll complex liposome is main is carried by lecithin, cholesterol, Tween-80, chlorophyll and fragrant camphor tree leaf Thing is taken to prepare.

Preferably, the chlorophyll complex liposome is counted by weight, including following component：60-80 parts of lecithin, courage 12-16 parts of sterol, 18-25 parts of Tween-80,4-8 parts of chlorophyll and fragrant 5-10 parts of camphor tree leaf extract.

Preferably, the preparation method of the chlorophyll complex liposome comprises the following steps：

S1：Lecithin, cholesterol, Tween-80, chlorophyll and fragrant camphor tree leaf extract are taken, is added by solid-to-liquid ratio 15-20mg/mL Enter absolute ethyl alcohol, stir it is molten make fully dissolving, be then 38-42 DEG C, under vacuum condition in temperature, rotary evaporation removes ethanol, Form film；

S2：PBS solution is added by solid-to-liquid ratio 0.07-0.1g/mL, normal pressure rotation 40-50min carries out washing film, obtains liposome Solution；

S3：Liposome solutions are taken, 3-5 is handled under conditions of pressure is 130-150MPa with dynamic high-pressure microjet instrument It is secondary, obtain chlorophyll composite liposome body.

Preferably, in the preparation method of the chlorophyll complex liposome, PBS pH described in step S2 is 7.2-8.0, dense Spend for 0.04-0.06mol/L.

Preferably, the preservative is glycerol caprylate, 1,2- hexylene glycols and fragrant camphor tree leaf extract in mass ratio 1:2-6: The compound preservative of 0.1-0.3 compositions.

Preferably, the preparation method of the fragrant camphor tree leaf extract comprises the following steps：Purebred fragrant camphor tree fresh leaf is taken, impurity elimination is washed Only, shredded after being dried under normal temperature, be 0.2-0.5MPa in steam pressure, temperature is steam treatment 10- under conditions of 105-110 DEG C 15s, cooling；It is 85-95% ethanol immersion 2-4h to add the volume fraction that 18-25 times of its weight measures, and is 35-45 DEG C in temperature Under conditions of ultrasound extraction 30-45min, filtering, filtrate decompression be distilled to recover ethanol, being concentrated under reduced pressure into relative density at 60 DEG C is 1.15-1.20, obtains fragrant camphor tree leaf extract.

Preferably, in the preparation method of the fragrant camphor tree leaf extract, the volume fraction of ethanol is 90%.

A kind of chlorophyll is sun-proof and after-sun skin care item, is prepared by the above method.

Compared with prior art, the beneficial effects of the invention are as follows：

1) chlorophyll that the present invention is provided is sun-proof and after-sun skin care item can be effective to the ultraviolet of different-waveband Defence, while also having good anti-light aging function, can prevent the symptoms such as skin aging caused by Exposure to Sunlight, spot, decortication, promote Enter the after-sun of skin；

2) chlorophyll that the present invention is provided is sun-proof and after-sun skin care item formula is reasonable, and the product property prepared is steady It is fixed, action temperature and, no skin irritation, comfort is good；

3) present invention provide containing chlorophyll of the present invention is sun-proof and after-sun skin care item preparation method simply, technique is steady It is fixed, it is convenient to carry out, can industrialized production, be conducive to popularization and application.

Embodiment

The present invention is further described below by way of embodiment, but the present invention is not limited only to following examples.This In inventive embodiments unless otherwise instructed, raw materials used is conventional commercial product, and such as chlorophyll is purchased from Haining Feng Ming chlorophyll Co., Ltd's (article No. is 65963-40-8), moringa oleifera leaf extractive is purchased from Xi'an Bioisystech Co., Ltd of Tianrui.

The preparation of the fragrant camphor tree leaf extract of embodiment 1

Purebred fragrant camphor tree fresh leaf is taken, impurity elimination is cleaned, and is shredded after being dried under normal temperature, be 0.2MPa in steam pressure, temperature is 105 Steam treatment 15s under conditions of DEG C, cooling；It is 90% ethanol immersion 3h to add the volume fraction of 20 times of amounts of its weight, in temperature Spend and extract 45min for ultrasound under conditions of 40 DEG C, filtering, filtrate decompression is distilled to recover ethanol, be concentrated under reduced pressure at 60 DEG C relative Density is 1.20, obtains fragrant camphor tree leaf extract.

The preparation of the chlorophyll complex liposome of embodiment 2

Chlorophyll complex liposome described in the present embodiment, is counted by weight, including following component：70 parts of lecithin, courage 14 parts of sterol, 21 parts of Tween-80,6 parts of chlorophyll and fragrant 8 parts of camphor tree leaf extract.

Preparation method includes：

S1：Lecithin, cholesterol, Tween-80, chlorophyll and fragrant camphor tree leaf extract are taken, nothing is added by solid-to-liquid ratio 18mg/mL Water-ethanol, stir it is molten make fully dissolving, be then 40 DEG C, under vacuum condition in temperature, rotary evaporation removes ethanol, is formed thin Film；

S2：It is 7.5 to add pH by solid-to-liquid ratio 0.08g/mL, and concentration is 0.05mol/L PBS solution, normal pressure rotation 45min Film is washed in progress, obtains liposome solutions；

S3：Liposome solutions are taken, is handled 4 times under conditions of pressure is 140MPa with dynamic high-pressure microjet instrument, obtains leaf Green plain composite liposome body.

The preparation of the chlorophyll complex liposome of embodiment 3

The present embodiment chlorophyll complex liposome, is counted by weight, including following component：60 parts of lecithin, cholesterol 12 parts, 18 parts of Tween-80,8 parts of chlorophyll and fragrant 10 parts of camphor tree leaf extract.

Preparation method：

S1：Lecithin, cholesterol, Tween-80, chlorophyll and fragrant camphor tree leaf extract are taken, nothing is added by solid-to-liquid ratio 20mg/mL Water-ethanol, stir it is molten make fully dissolving, be then 40 DEG C, under vacuum condition in temperature, rotary evaporation removes ethanol, is formed thin Film；

S2：It is 7.4 to add pH by solid-to-liquid ratio 0.1g/mL, and concentration is 0.04mol/LPBS solution, and normal pressure rotation 50min enters Row washes film, obtains liposome solutions；

S3：Liposome solutions are taken, is handled 5 times under conditions of pressure is 130MPa with dynamic high-pressure microjet instrument, obtains leaf Green plain composite liposome body.

The chlorophyll of the present invention of embodiment 4 is sun-proof and preparations of after-sun skin care item

1) hydrogenated jojoba oil 4%, amygdalus pedunculata pall oil 4%, saualane 3%, the poly- diformazan of ring five by weight percentage, are taken Radical siloxane 3%, cetostearyl alcohol 2%, PEG-100 stearates 1.5%, tocopherol acetate 0.8%, bisabolol 0.2% and cetearyl glucoside 3% add oil cauldron in, be heated to 80 DEG C, constant temperature is stirred to being completely dissolved, and obtains material A；

2) by weight percentage, take butanediol 6%, Sodium Hyaluronate 0.05%, carbomer 0.2%, trehalose 2%, VCE 0.6% and water 57.94% are added in aqueous phase pot, are heated to 80 DEG C, constant temperature is stirred to after being completely dissolved It is pumped into warmed-up emulsifying pot, opens stirring, material A is slowly pumped into emulsification pot, carry out emulsifying, stirring turns Speed is 2500r/min, and the emulsifying time is 7min, and vacuum defoamation starts cooling；

3) when being cooled to 42 DEG C, by weight percentage, add embodiment 2 prepare chlorophyll complex liposome 7%, β- Glucan 0.3%, preservative 1.2%, moringa oleifera leaf extractive 3%, triethanolamine 0.2% and essence 0.01%, are carried out second Emulsifying, speed of agitator is 600r/min, and the emulsifying time is 3min, and room temperature is down in vacuum defoamation, and sampling Detection is closed Lattice discharge, and produce.

Wherein, the preservative is glycerol caprylate, 1,2- hexylene glycols and fragrant camphor tree leaf extract in mass ratio 1:3:0.2 group Into compound preservative.

The chlorophyll of the present invention of embodiment 5 is sun-proof and preparations of after-sun skin care item

1) hydrogenated jojoba oil 2%, amygdalus pedunculata pall oil 2%, saualane 5%, the poly- diformazan of ring five by weight percentage, are taken Radical siloxane 3%, cetostearyl alcohol 1%, PEG-100 stearates 2%, tocopherol acetate 0.5%, bisabolol 0.3% Added with cetearyl glucoside 3% in oil cauldron, be heated to 80 DEG C, constant temperature is stirred to being completely dissolved, and obtains material A；

2) butanediol 5%, Sodium Hyaluronate 0.05%, carbomer 0.4%, trehalose by weight percentage, are taken 0.5%th, VCE 0.2% and water 67.89% are added in aqueous phase pot, are heated to 80 DEG C, constant temperature is stirred to complete It is pumped into after dissolving in warmed-up emulsifying pot, opens stirring, material A is slowly pumped into emulsification pot, carries out emulsifying, Speed of agitator is 3000r/min, and the emulsifying time is 5min, and vacuum defoamation starts cooling；

3) when being cooled to 40 DEG C, by weight percentage, add embodiment 2 prepare chlorophyll complex liposome 5%, β- Glucan 0.4%, preservative 0.6%, moringa oleifera leaf extractive 1%, triethanolamine 0.15% and essence 0.01%, are carried out second Emulsifying, speed of agitator is 500r/min, and the emulsifying time is 4min, and room temperature is down in vacuum defoamation, and sampling Detection is closed Lattice discharge, and produce.

Wherein, the preservative is glycerol caprylate, 1,2- hexylene glycols and fragrant camphor tree leaf extract in mass ratio 1:5:0.1 group Into compound preservative.

The chlorophyll of the present invention of embodiment 6 is sun-proof and preparations of after-sun skin care item

1) hydrogenated jojoba oil 6%, amygdalus pedunculata pall oil 6%, saualane 5%, the poly- diformazan of ring five by weight percentage, are taken Radical siloxane 3%, cetostearyl alcohol 1.5%, PEG-100 stearates 1.5%, tocopherol acetate 1%, bisabolol 0.1% and cetearyl glucoside 4% add oil cauldron in, be heated to 80 DEG C, constant temperature is stirred to being completely dissolved, and obtains material A；

2) butanediol 7%, Sodium Hyaluronate 0.2%, carbomer 0.1%, trehalose 3%, dimension by weight percentage, are taken Raw element C ethylethers 0.6% and water 44.69% are added in aqueous phase pot, are heated to 80 DEG C, constant temperature is stirred to after being completely dissolved and taken out Enter in warmed-up emulsifying pot, open stirring, material A is slowly pumped into emulsification pot, carries out emulsifying, speed of agitator For 3000r/min, the emulsifying time is 8min, and vacuum defoamation starts cooling；

3) when being cooled to 40 DEG C, by weight percentage, the chlorophyll complex liposome 10% of the addition preparation of embodiment 3, Beta glucan 0.5%, preservative 1.5%, moringa oleifera leaf extractive 4%, triethanolamine 0.3% and essence 0.01%, carry out second Secondary emulsifying, speed of agitator is 700r/min, and the emulsifying time is 2min, vacuum defoamation, is down to room temperature, sampling Detection, Qualified discharging, is produced.

Wherein, the preservative is glycerol caprylate, 1,2- hexylene glycols and fragrant camphor tree leaf extract in mass ratio 1:2:0.3 group Into compound preservative.

Comparative example 1

1) chlorophyll complex liposome is prepared：Count by weight, including following component：70 parts of lecithin, cholesterol 14 Part, 21 parts of Tween-80,6 parts of chlorophyll.

Preparation method be the same as Example 2 is similar.

2) prepare that chlorophyll is sun-proof and after-sun skin care item：Step 3) in addition chlorophyll complex liposome for this is right The chlorophyll complex liposome that ratio is prepared, remaining step be the same as Example 4.

The difference of comparative example 1 and embodiment 4 is, without fragrant camphor tree leaf extract in the chlorophyll complex liposome.

Comparative example 2

The chlorophyll of comparative example 2 is sun-proof and preparation methods of after-sun skin care item, step 2) in, by weight percentage, The addition of water be 60.94%, step 3) in without moringa oleifera leaf extractive, remaining be the same as Example 4.

Comparative example 3

The chlorophyll of comparative example 3 is sun-proof and preparation methods of after-sun skin care item, step 2) in, by weight percentage, The addition of water is 58.54%, without VCE, remaining be the same as Example 4.

Comparative example 4

This comparative example chlorophyll is sun-proof and preparation methods of after-sun skin care item：

2) by weight percentage, take butanediol 6%, Sodium Hyaluronate 0.05%, carbomer 0.2%, trehalose 2%, VCE 0.6% and water 64.09% are added in aqueous phase pot, are heated to 80 DEG C, constant temperature is stirred to after being completely dissolved It is pumped into warmed-up emulsifying pot, opens stirring, material A is slowly pumped into emulsification pot, carry out emulsifying, stirring turns Speed is 2500r/min, and the emulsifying time is 7min, and vacuum defoamation starts cooling；

3) when being cooled to 42 DEG C, by weight percentage, chlorophyll 0.35%, fragrant camphor tree leaf extract 0.5%, β-Portugal are added Glycan 0.3%, preservative 1.2%, moringa oleifera leaf extractive 3%, triethanolamine 0.2% and essence 0.01%, are carried out equal for the second time Matter is emulsified, and speed of agitator is 600r/min, and the emulsifying time is 3min, and room temperature is down in vacuum defoamation, and sampling Detection is qualified Discharging, is produced.

The chlorophyll of the present invention of test example one is sun-proof and after-sun skin care item estimation of stability

1st, test specimen：The chlorophyll that embodiment 4-6, comparative example 1-4 are prepared is sun-proof and after-sun skin care item.

2nd, test method：

1) heat-resistant stability：Sample is placed under (40 ± 1 DEG C) temperature conditionss, respectively at after 24h, 7d, 30d, recovery room The change of temperature observation sample is with the heat-resistant stability of judgement sample.

2) resist cold stability：Sample is placed under (- 8 ± 2 DEG C) temperature conditionss, respectively at after 24h, 7d, 30d, recovery room The change of temperature observation sample is with the cold-resistant stability of judgement sample.

3) light durability：Sample is placed in sunlight irradiation position, sample is observed respectively at after 24h, 7d, 30d, recovering room temperature Change with the light durability of judgement sample.

3rd, result of the test：It is shown in Table 1.

The chlorophyll of table 1 is sun-proof and after-sun skin care item stability test result

From the result of the test of table 1：

(1) chlorophyll that 4-6 of the embodiment of the present invention is prepared is sun-proof and after-sun skin care item pass through heat-resistant stability After experiment, cold-resistant stability test and light durability experiment, its tissue morphology does not occur obvious change, illustrates the present invention The chlorophyll of offer is sun-proof and after-sun skin care item stability is good.

(2) chlorophyll that comparative example 1 and comparative example 4 are prepared is sun-proof and less stables of after-sun skin care item, Explanation prepares chlorophyll liposome and not only contributes to improve dissolubility of the chlorophyll in cosmetics, while also helping chlorophyll Sun-proof and after-sun skin care item stable systems and the stability for improving chlorophyll, and chlorophyll is answered with fragrant camphor tree leaf extract With using, be conducive to further improving the stability of chlorophyll.

(3) chlorophyll that comparative example 2 and comparative example 3 are prepared is sun-proof and stability test knots of after-sun skin care item Fruit explanation chlorophyll is sun-proof and after-sun skin care item system in addition moringa oleifera leaf extractive and VCE it is favourable In the stability and the stability of chlorophyll of the system of raising.

The chlorophyll of the present invention of test example two is sun-proof and after-sun skin care item safety evaluatio

With reference to the Ministry of Public Health《Cosmetics health specification》In (version in 2007) related request of " human skin patch " and Assay method is sun-proof to the chlorophyll that the present invention implements 4-6, comparative example 1-4 is prepared and after-sun skin care item are surveyed Examination, the experimental result of all trial volunteers is negative findings, illustrates that the present invention implements 4-6, comparative example 1-4 and prepared Chlorophyll is sun-proof and after-sun skin care item are without skin adverse reaction.The chlorophyll that the present invention is provided is sun-proof and after-sun skin care Product action temperature and, no skin irritation is safe.

The chlorophyll of the present invention of test example three is sun-proof and after-sun skin care item SPF is determined

With reference to the Ministry of Public Health《Cosmetics health specification》" sun care preparations SPF (spf value) is determined in (version in 2007) The related request and assay method of method " and " sun care preparations long wave ultraviolet protection index (PFA values) assay method " are carried out. Given the test agent is that the chlorophyll for preparing of 4-6 of the embodiment of the present invention, comparative example 1-4 is sun-proof and after-sun skin care item.Twice Experiment 105 trial volunteers of each selection are tested, and are randomly divided into 7 groups, every group of 15 people.Result of the test is shown in Table 2.

The chlorophyll of the present invention of table 2 is sun-proof and the sun-proof the performance test results of after-sun skin care item

Group	Embodiment 4	Embodiment 5	Embodiment 6	Comparative example 1	Comparative example 2	Comparative example 3	Comparative example 4
								Spf value	30	27	28	14	18	20	22
PFA values	10	9	10	3	5	5	6
								PA grades	PA+++	PA+++	PA+++	PA+	PA++	PA++	PA++

From the test result of table 2：

(1) chlorophyll that 4-6 of the embodiment of the present invention is prepared is sun-proof and after-sun skin care item spf value is more than 25, PA grades reach PA+++, illustrate that the chlorophyll of the invention provided is sun-proof and after-sun skin care item are to the ultraviolet of different-waveband Line can be defendd effectively, and sun-proof scope is wide, and sun-proof result is notable.

(2) chlorophyll that comparative example 1 is prepared is sun-proof and after-sun skin care item, and sun-proof performance is compared with Example 4 With significant difference, illustrate that chlorophyll can effectively improve the sun-proof performance of sunlight screening skin-protecting product with fragrant camphor tree leaf extract compounding use. Containing compositions such as camphor tree lignanoid, camphorwood flavones, camphorwood polyphenol, Quercetins in fragrant camphor tree leaf extract, with certain uvioresistant Effect, while also having significant anti-oxidant, promoting blood circulation, anti-inflammation and sterilization, moisturizing and the effect of skin nutrition, can prevent acne, sparrow The appearance of spot and melanin deposition, accelerates the recovery of skin histology, is conducive to the after-sun of skin.

(3) the test result explanation of comparative example 2 and comparative example 3, moringa oleifera leaf extractive and VCE with other into Point synergy is sun-proof to chlorophyll of the present invention and sun-proof performances of after-sun skin care item have and certain improve effect.This Outside, moringa oleifera leaf extractive is rich in several mineral materials, vitamin and amino acid, with it is anti-oxidant, alleviate pruitus, allergy and wet Effect of rash, can effectively facilitate the after-sun of skin.VCE (No. CAS：86404-04-8) it is a kind of vitamin C derivatives, are usually used in whitening and freckle-removing product, with significant whitening effect, meanwhile, it also there is stronger anti-inflammation to make With, applied to chlorophyll of the present invention is sun-proof and after-sun skin care item in can prevent inflammation caused by skin tanning and antisum.

The chlorophyll of the present invention of test example four is sun-proof and after-sun skin care item sensory evaluation

1st, given the test agent：The chlorophyll that 4-6 of the embodiment of the present invention is prepared is sun-proof and after-sun skin care item.

2nd, test method：Choose 90 women trial volunteers and participate in experiment, age 20-45 Sui, volunteer's exclusion condition Meet《Cosmetics contact dermatitis diagnostic criteria and treatment principle》Include, exclusion standard.All trial volunteers are divided at random Into 3 groups of embodiment 4-6 groups, every group of 30 people.Then each group trial volunteer uses corresponding given the test agent respectively, continuously using 4 Week.Ask each trial volunteer sun-proof to chlorophyll of the present invention after off-test and after-sun skin care item common sensation, absorb Effect, stretchability, moisturizing effect, exquisiteness, glossiness, viscosity, glutinous soapy feeling, salubrious degree and pick do sensory evaluation, often Item, which is evaluated, carries out scoring mechanism, and 9-10 points are fine, and 7-8 points are preferable, and it is general that 5-6, which divides, and 3-4 points are poor, and 1-2 points is very Difference.Result of the test is shown in Table 3.

The chlorophyll of the present invention of table 3 is sun-proof and after-sun skin care item Analyses Methods for Sensory Evaluation Results

As shown in Table 3, the chlorophyll that the present invention is provided is sun-proof and the sensory evaluation of after-sun skin care item is high, uses Comfortableness is good.

Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to assert The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention, On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's Protection domain.

Claims

1. a kind of chlorophyll is sun-proof and preparation methods of after-sun skin care item, it is characterised in that comprise the following steps：

1) hydrogenated jojoba oil 2-6%, amygdalus pedunculata pall oil 2-6%, saualane 2-5%, ring five poly- two by weight percentage, are taken Methylsiloxane 2-5%, cetostearyl alcohol 1-3%, PEG-100 stearate 1-2%, tocopherol acetate 0.5-1%, red do not have Medicine alcohol 0.1-0.3% and cetearyl glucoside 2-4% is added in oil cauldron, is heated to 80-85 DEG C, constant temperature is stirred to complete Fully dissolved, obtains material A；

2) butanediol 3-10%, Sodium Hyaluronate 0.05-0.2%, carbomer 0.1-0.4%, marine alga by weight percentage, are taken Sugared 0.5-3%, VCE 0.2-0.8% and water 38-75% are added in aqueous phase pot, are heated to 80-85 DEG C, perseverance Temperature stirring is pumped into after being completely dissolved in warmed-up emulsifying pot, opens stirring, material A is slowly pumped into emulsification pot, Emulsifying is carried out, speed of agitator is 2500-3000r/min, and the emulsifying time is 5-10min, and vacuum defoamation starts drop Temperature；

3) when being cooled to 40-45 DEG C, by weight percentage, chlorophyll complex liposome 5-10%, beta glucan 0.2- are added 0.5%th, preservative 0.6-1.5%, moringa oleifera leaf extractive 1-4%, triethanolamine 0.1-0.4% and essence 0.01-0.1%, enter Second of emulsifying of row, speed of agitator is 500-700r/min, and the emulsifying time is 2-5min, and room is down in vacuum defoamation Temperature, sampling Detection, qualified discharging is produced；

Wherein, the chlorophyll complex liposome is counted by weight, including following component：60-80 parts of lecithin, cholesterol 12-16 parts, 18-25 parts of Tween-80,4-8 parts of chlorophyll and fragrant 5-10 parts of camphor tree leaf extract；The system of the fragrant camphor tree leaf extract Preparation Method comprises the following steps：Purebred fragrant camphor tree fresh leaf is taken, impurity elimination is cleaned, and is shredded after being dried under normal temperature, be 0.2- in steam pressure 0.5MPa, temperature is steam treatment 10-15s under conditions of 105-110 DEG C, cooling；Add the volume integral that 18-25 times of its weight is measured Number soaks 2-4h for 85-95% ethanol, and the ultrasound extraction 30-45min under conditions of temperature is 35-45 DEG C, filtering, filtrate subtracts Pressure is distilled to recover ethanol, and it is 1.15-1.20 to be concentrated under reduced pressure into relative density at 60 DEG C, obtains fragrant camphor tree leaf extract；

The preparation method of the chlorophyll complex liposome comprises the following steps：

S1：Lecithin, cholesterol, Tween-80, chlorophyll and fragrant camphor tree leaf extract are taken, nothing is added by solid-to-liquid ratio 15-20mg/mL Water-ethanol, stir it is molten make fully dissolving, be then 38-42 DEG C, under vacuum condition in temperature, rotary evaporation removes ethanol, is formed Film；

S2：PBS solution is added by solid-to-liquid ratio 0.07-0.1g/mL, normal pressure rotation 40-50min carries out washing film, obtains liposome solutions；

S3：Liposome solutions are taken, is handled 3-5 times, obtained under conditions of pressure is 130-150MPa with dynamic high-pressure microjet instrument Chlorophyll composite liposome body.

2. chlorophyll as claimed in claim 1 is sun-proof and preparation methods of after-sun skin care item, it is characterised in that the leaf In the preparation method of green plain complex liposome, PBS pH described in step S2 is 7.2-8.0, and concentration is 0.04-0.06mol/L.

3. chlorophyll as claimed in claim 1 is sun-proof and preparation methods of after-sun skin care item, it is characterised in that step 3) Described in preservative be glycerol caprylate, 1,2- hexylene glycols and fragrant camphor tree leaf extract in mass ratio 1:2-6:0.1-0.3 compositions Compound preservative.

4. chlorophyll as claimed in claim 1 is sun-proof and preparation methods of after-sun skin care item, it is characterised in that the virtue In the preparation method of camphor tree leaf extract, the volume fraction of ethanol is 90%.

The leaf that 5. chlorophyll as described in claim 1-4 is any is sun-proof and preparation methods of after-sun skin care item are prepared Green element is sun-proof and after-sun skin care item.