CN106489929A - A kind of exhibition film oil-suspending agent and its application in terms of preventing and treating crops sucking pest - Google Patents
A kind of exhibition film oil-suspending agent and its application in terms of preventing and treating crops sucking pest Download PDFInfo
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- CN106489929A CN106489929A CN201610799878.5A CN201610799878A CN106489929A CN 106489929 A CN106489929 A CN 106489929A CN 201610799878 A CN201610799878 A CN 201610799878A CN 106489929 A CN106489929 A CN 106489929A
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- suspending agent
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
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- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
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Abstract
A kind of exhibition film oil-suspending agent and its application in terms of preventing and treating crops sucking pest.The invention provides a kind of exhibition film oil-suspending agent containing Paecilomyces lilacinus, including the component of following weight percentage：Effective ingredient 0.4～50%, the effective ingredient include Paecilomyces lilacinus；Surfactant 3.0～25%, film former 0.6～20%, balance of oiliness disperse medium.In the present invention, there is provided Paecilomyces lilacinus exhibition film oil-suspending agent to the prevention effect of sucking pest significantly, prevention effect is up to 83～85%.The exhibition film oil-suspending agent of the Paecilomyces lilacinus that the present invention is provided is compared tradition exhibition film oil-suspending agent and is worked well, and opens up spread excellent performance of the film oil-suspending agent in liquid level.
Description
Technical field
The present invention relates to biological pesticide technical field, more particularly to a kind of exhibition film oil-suspending agent and its in preventing and treating crops thorn
Application in terms of suction mouthpart insect.
Background technology
Film-spreading oil solution is to impose on the oil preparation that the water surface forms thin film, and with prevention effect outstanding feature, dart transfer is prevented and treated
Expense is low, and compared with other pesticide, consumption and cost are all relatively low.And in use, easy to operate, without plant protection device
Tool, directly instills medicine in water, improves 20 times of work efficiency than conventional dispenser, greatly mitigates dispenser labor intensity, is conducive to labour to turn
Change, change existing traditional heavy application method.In addition no matter the impact of the dosage form not climate, rain or cloudy in Control stage
It can directly drip medicine.The dosage form belongs to low toxicity dosage form, due to special insecticide-applying way so as to do not produce medicine in use
Liquid drift, free from environmental pollution, poisoning is not produced, little to natural enemy lethality, to person poultry safety.
And the dosage form product of film-spreading oil solution need not be watered dilution, directly spread
Open, form one layer of uniform medicine film, while in the presence of auxiliary agent, medicine film can be sprawled upwards along crop stem, concentrate on water
Plant stalk base portion, so that realize effective preventing and treating of aquatic crops disease pest and weed that conventional insecticide-applying way is difficult to.
However, the species of existing film-spreading oil solution is not good enough in water surface spreading property, the preventing and treating of the medicine film performance of formation to product
Effect has larger fluctuating.
Content of the invention
In view of this, present invention aims to the deficiencies in the prior art, and provide a kind of containing Paecilomyces lilacinus
Exhibition film oil-suspending agent.
In order to realize that foregoing invention purpose, the present invention provide technical scheme below：
The invention provides a kind of exhibition film oil-suspending agent, including the component of following weight/mass percentage composition：Active component 0.4～
50%, described active component includes Paecilomyces lilacinus；Surfactant 3～25%；Film former 0.6～25%；The oil of surplus
Property disperse medium.
Preferably, in the Paecilomyces lilacinus, spore content living is 1～5,000,000,000/gram.
Preferably, described active component also includes organic insecticide, and described organic insecticide is methylamino Avermectin
Element, MTI-446, Diacloden, avilamycin, thiacloprid, pleocidin, flonicamid, d ichlorbenzuron, Rynaxypyr, deinsectization
One or more in urea, effective cypermethrin, gamma cyhalothrin, decises and indoxacarb.
Preferably, the Paecilomyces lilacinus are (1～40) with the weight ratio of organic insecticide：(1～50).
Preferably, the film former include Lauryl Alcohol ester, octadecanol, Propylene Glycol phenylate, dibutyl phthalate and
One or more in monobutyl ether acetate.
Preferably, weight/mass percentage composition of the film former in exhibition film oil-suspending agent is 3～20%.
Preferably, the surfactant includes iso-octyl sodium succinate and the one kind in fatty alcohol-polyoxyethylene ether or two
Kind.
Preferably, the surfactant including 5～20%.
Preferably, the oiliness disperse medium is the one kind in white oil, dimethylbenzene, solvent naphtha, methyl naphthalene and ethyl acetate
Or it is several.
Further, the invention provides described exhibition film oil-suspending agent is in terms of preventing and treating crops sucking pest
Application.
A kind of exhibition film oil-suspending agent containing Paecilomyces lilacinus that the present invention is provided, including the group of following weight percentage
Point：Effective ingredient 0.4～50%, the effective ingredient include Paecilomyces lilacinus；Surfactant 3～25%；Film former 0.6～
25%；Balance of oiliness disperse medium.In the present invention, there is provided Paecilomyces lilacinus exhibition film oil-suspending agent to sucking mouth parts
Significantly, prevention effect is up to 83～85% for the prevention effect of insect.The exhibition film oil-suspending agent of the Paecilomyces lilacinus that the present invention is provided
Compare tradition exhibition film oil-suspending agent to work well, open up spread excellent performance of the film oil-suspending agent in liquid level.
Biological deposits are proved
Paecilomyces lilacinus (Paecilomyces lilacinus), were deposited in China Microbiological bacterium on 08 13rd, 2013
Kind of preservation administration committee common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the Chinese Academy of Sciences
Institute of microbiology, biological deposits numbering is CGMCC No.7994.
Specific embodiment
The invention provides a kind of exhibition film oil-suspending agent, including the component of following weight/mass percentage composition：Active component 0.4～
50%, described active component includes Paecilomyces lilacinus；Surfactant 3～25%；Film former 0.6～25%；The oil of surplus
Property disperse medium.
The mass content of active component described in the present invention is preferably 0.4～50%, more preferably 8～35%, most
Preferably 15～25%.Heretofore described active component includes Paecilomyces lilacinus, spore living in described Paecilomyces lilacinus
Content is preferably 1～5,000,000,000/gram, most preferably more preferably 2～2,000,000,000/gram, 500,000,000/gram.
In the present invention, the Paecilomyces lilacinus are preferably selected from the strain that biological deposits numbering is CGMCC No.7994
Activation culture is obtained.
In the present invention, the preparation method for carrying pale purple penicillium sp is comprised the following steps：
1) Paecilomyces lilacinus strain is activated；
2) by the step 1) Paecilomyces lilacinus after the activation that obtains are inoculated in Paecilomyces lilacinus level liquid culture medium
In carry out level liquid culture, obtain Paecilomyces lilacinus primary seed solution；
3) by the step 2) primary seed solution that obtains is inoculated in Paecilomyces lilacinus secondary liquid culture medium and carries out two
Level liquid culture, obtains Paecilomyces lilacinus secondary seed solution；
4) by the step 3) secondary seed solution that obtains is inoculated in Paecilomyces lilacinus solid medium, carries out solid training
Support, obtain Paecilomyces lilacinus.
In the present invention, the activation of Paecilomyces lilacinus strain is that the Paecilomyces lilacinus strain of preservation is inoculated in equipped with nutrition fine jade
In the eggplant type bottle of fat culture medium, 25～30 DEG C of 10～14h of culture carry out activation culture.
The Paecilomyces lilacinus for activating are obtained, the Paecilomyces lilacinus of the activation are inoculated into Paecilomyces lilacinus one-level by the present invention
Paecilomyces lilacinus level liquid culture is carried out in fluid medium, obtains Paecilomyces lilacinus primary seed solution.In the present invention, institute
The inoculum concentration for stating level liquid culture is preferably 0.5～1.5%, more preferably 0.8～1.2%, most preferably 1.0%.
In the present invention, the level liquid culture medium preferably includes the component of following mass content：Glucose 30～
50g/L, 5～15g/L of analysis for soybean powder, 5～15g/L of sodium nitrate, 1.8～2.2g/L of dipotassium hydrogen phosphate, 0.25～0.75g/ of potassium chloride
L, 0.18～0.30g/L of magnesium sulfate, 0.01～0.08g/L of iron sulfate, balance of water；More preferably 35～45g/L of glucose, yellow
8～12g/L of Semen Glycines powder, 8～12g/L of sodium nitrate, 1.9～2.15g/L of dipotassium hydrogen phosphate, 0.35～0.65g/L of potassium chloride, magnesium sulfate
0.20～0.26g/L, 0.03～0.07g/L of iron sulfate, balance of water；Most preferably glucose 40g/L, analysis for soybean powder 10g/L, nitre
Sour sodium 10g/L, dipotassium hydrogen phosphate 2.06g/L, potassium chloride 0.5g/L, magnesium sulfate 0.24g/L, iron sulfate 0.05g/L, balance of
Water.The present invention is not particularly limited to the source of above-mentioned medicine, is conventionally used for preparing culture medium using those skilled in the art
Medicine.
In the present invention, the time of the level liquid culture is preferably 35～50h, more preferably 40～48h, most preferably
For 43h.The temperature of the level liquid culture is preferably 25～30 DEG C, more preferably 26～29 DEG C, most preferably 28 DEG C.Described
The original ph of level liquid culture preferably 6.0～7.0, more preferably 6.4～6.8, most preferably 6.5.
In the present invention, the process in level liquid culture is ventilated, and is particularly preferred as：Enter in culture 24h, ventilation
Measure as 10～20m3/ h, after culture 24h, ventilation is 15～24m3/h；More preferably：Enter in culture 24h, ventilation is 12
～18m3/ h, after culture 24h, ventilation is 18～22m3/h；Most preferably：Enter in culture 24h, ventilation is 15m3/ h, training
After foster 24h, ventilation is 20m3/h.The present invention is not particularly limited to the equipment of the ventilation, using those skilled in the art
Well known aeration equipments, such as ventilation blower.
In the present invention, the level liquid culture is cultivated under dark condition.The present invention is to providing dark condition
Method be not particularly limited, the method for the offer dark condition that is routinely selected using those skilled in the art.
After obtaining Paecilomyces lilacinus primary seed solution, the one-level Paecilomyces lilacinus primary seed solution is inoculated in by the present invention
Paecilomyces lilacinus secondary liquid culture is carried out in Paecilomyces lilacinus secondary liquid culture medium, obtains Paecilomyces lilacinus secondary seed
Liquid.In the present invention, the inoculum concentration of the secondary liquid culture is preferably 5～15%, more preferably 8～12%, most preferably
10%
In the present invention, the secondary liquid culture medium preferably includes the component of following mass content：10～80g/ of sucrose
L, 1～30g/L of analysis for soybean powder, 0.1～20g/L of sodium nitrate, 0.05～5g/L of dipotassium hydrogen phosphate, 0.03～3g/L of zinc sulfate, sulphuric acid
0.01～1g/L of ferrum, balance of water；More preferably 25～60g/L of sucrose, 8～22g/L of analysis for soybean powder, 3～15g/L of sodium nitrate, phosphorus
0.5～3g/L of sour hydrogen dipotassium, 0.1～2g/L of zinc sulfate, 0.03～0.07g/L of iron sulfate, balance of water；Most preferably sucrose
40g/L, analysis for soybean powder 20g/L, sodium nitrate 5g/L, dipotassium hydrogen phosphate 1.5g/L, zinc sulfate 0.24g/L, iron sulfate 0.05g/L are remaining
Measure as water.The present invention is not particularly limited to the source of above-mentioned medicine, is conventionally used for preparing culture using those skilled in the art
The medicine of base.
In the present invention, the time of the Paecilomyces lilacinus secondary liquid culture preferably 55～75h, more preferably 60～
70h, most preferably 65h；The temperature of the secondary liquid culture is preferably 25～30 DEG C, more preferably 26～29 DEG C, most preferably
For 28 DEG C；The original ph of the secondary liquid culture is preferably 6.0～7.0, more preferably 6.3～6.7, most preferably 6.5.
In the present invention, air is passed through in secondary liquid incubation, be particularly preferred as entering in culture 24h, ventilation
For 130～170m3/ h, after culture 24h, ventilation is 200～260m3/h；More preferably enter in culture 24h, ventilation is
140～160m3/ h, after culture 24h, ventilation is 210～250m3/h；Most preferably enter in culture 24h, ventilation is
150m3/ h, after culture 24h, ventilation is 230m3/h.The present invention does not have special restriction to the equipment of the ventilation, using this
Aeration equipments known to art personnel, such as ventilation blower.
In the present invention, the secondary liquid culture needs to carry out under agitation, and the frequency of the stirring is preferably 1～2
Secondary/h, more preferably 2 times/h；The time of stirring is preferably 10～20min, more preferably 12～18min every time, most preferably
15min.The present invention does not have special restriction to the equipment of the stirring, using mixing plant well known to those skilled in the art is
Can.
In the present invention, the secondary liquid culture is cultivated under dark condition.The present invention is to providing dark condition
Method be not particularly limited, the method for the offer dark condition that is routinely selected using those skilled in the art.
After obtaining Paecilomyces lilacinus secondary seed solution, the Paecilomyces lilacinus secondary seed solution for obtaining is inoculated in light by the present invention
In purple Paecilomyces varioti solid medium, solid culture is carried out, Paecilomyces lilacinus are obtained.In the present invention, the Paecilomyces lilacinus are solid
The inoculum concentration of body culture is preferably 5～15%, more preferably 8～12%, most preferably 10%.
In the present invention, the Paecilomyces lilacinus solid medium preferably includes the component of following mass content：Testa Tritici 40
～60%, Semen Maydis powder 25～35%, analysis for soybean powder 5～15%, rice husk 5～13%, ammonium sulfate 0.5～1.5%；More preferably Testa Tritici
45～55%, Semen Maydis powder 27～32%, analysis for soybean powder 8～12%, rice husk 7～11%, ammonium sulfate 0.8～1.2%；Most preferably wheat
Bran 50%, Semen Maydis powder 30%, analysis for soybean powder 10%, rice husk 9%, ammonium sulfate 1%.The present invention is not special to the source of above-mentioned raw materials
Restriction, the raw material for routinely configuring culture medium using those skilled in the art.
In the present invention, the initial water content of the Paecilomyces lilacinus solid medium is preferably 30～70%, more preferably
For 40～60%, most preferably 50%.In the present invention, the culture medium thickness of the solid culture are preferably 1～6cm, more excellent
Elect 2～4cm, most preferably 3cm as.
In the present invention, the control of the process temperature of the Paecilomyces lilacinus solid culture and humidity, the temperature and wet
The control program of degree is particularly preferred as：It is 80～90% in the inner control loop border humidity for entering 1～3d of solid culture, ambient temperature
For 24～28 DEG C, bacterium material temperature degree is controlled to 24～28 DEG C, enter 4～5d of solid culture inner control loop border humidity be 40～
50%, ambient temperature is 26 DEG C, and within 6～7d, ambient temperature is 28～30 DEG C；More preferably：Entering 1～3d of solid culture
Inner control loop border humidity be 85%, ambient temperature be 26 DEG C, bacterium material temperature degree is controlled to 26 DEG C, enter 4～5d of solid culture
Inner control loop border humidity be 45%, ambient temperature be 26 DEG C, within 6～7d ambient temperature be 29 DEG C, ambient humidity is nature
Under the conditions of humidity.
In the present invention, the Paecilomyces lilacinus solid culture preferably turns in 28～32h radiating, more preferably 29～
31h, most preferably 30h.
In the present invention, the Paecilomyces lilacinus solid culture preferably in 45～55h is sprayed onto nutritional solution on bacterium material,
More preferably 48～52h, most preferably 50h.The fountain height of the nutritional solution is preferably the 5～10% of bacterium material volume, more preferably
For 6～9%, most preferably 8%.The present invention is not particularly limited to the equipment for spraying nutritional solution, using those skilled in the art
Well known spray appliance.
In the present invention, the nutritional solution preferably includes the component of following mass content：5% glucose, 0.002%
CaCl2And 0.003%ZnSO4.The present invention is not particularly limited to the source of above-mentioned medicine, using those skilled in the art's routine
The medicine for using.
After the solid culture, preferred pair of the present invention obtains being collected for spore powder, obtains Paecilomyces lilacinus spore.?
In the present invention, the collection of the spore powder is specifically carried out using the instrument of microorganism solid fermentation fungal spore separation equipment.Institute
The instrument for stating microorganism solid fermentation fungal spore separation equipment is purchased from Jiangxi Tianren Ecology Co., Ltd..
In the present invention, described active component preferably also includes organic insecticide；Specifically the organic insecticide is excellent
Elect as Affirm (Merck Co.), MTI-446, Diacloden, avilamycin, thiacloprid, pleocidin, flonicamid, d ichlorbenzuron,
One kind or several in Rynaxypyr, diflubenzuron, effective cypermethrin, gamma cyhalothrin, decises and indoxacarb
Kind.The present invention is not particularly limited to the source of above-mentioned organic insecticide, using commercial goods.
In the present invention, when described active component includes Paecilomyces lilacinus and organic insecticide simultaneously, pale purple plan is blue or green
The mould mass ratio with organic insecticide is preferably (1～40)：(1～50), more preferably (3～38)：(2～45), most preferably
(8～30)：(10～35).
The exhibition film oil-suspending agent that the present invention is provided includes that mass content is preferably 3～25% surfactant, more preferably
For 5～20%, most preferably 12～16%.In the present invention, described surfactant is not particularly limited, using ability
Commercial goods known to field technique personnel.Specific is preferably in iso-octyl sodium succinate and fatty alcohol-polyoxyethylene ether
One or two, more preferably iso-octyl sodium succinate and fatty alcohol-polyoxyethylene ether.When the surfactant is above-mentioned
During two kinds of reagents, the iso-octyl sodium succinate is preferably (1～10) with the mass ratio of fatty alcohol-polyoxyethylene ether：5, more preferably
For (3～7)：5, most preferably 5：5.In the present invention, the SURFACTANT ADSORPTION is formed different in pesticide microparticle surfaces
Dispersion, plays emulsifying, moistening, solubilising, froth breaking, stable effect.During Pesticide use, surfactant can change
Distribution of the kind medicinal liquid on plant leaf surface or controlling object surface, attachment, infiltration etc., improve the effective transfer of pesticide dosage, directly
Connect or improve indirectly the effective rate of utilization of pesticide.
The exhibition film oil-suspending agent that the present invention is provided preferably includes the film former that mass content is 0.6～25%, and more preferably 3
～20%, most preferably 10～15%.In the present invention, the source of the film former is not particularly limited, using this area
The commercial goods that technical staff routinely selects.Specific is preferably Lauryl Alcohol ester, octadecanol, Propylene Glycol phenylate, neighbour
One or more in phthalic acid dibutyl ester and monobutyl ether acetate, more preferably 2～4 kinds, most preferably 5 kinds.When described into
When membrane is 2～4 kinds of mentioned reagent, can arbitrary proportion mixing.When 5 kinds that the film former is mentioned reagent, described ten
Two carbon alcohol esters and octadecanol, Propylene Glycol phenylate, dibutyl phthalate, the mass ratio of monobutyl ether acetate be preferably (0.5～
8)：(1～8)：(0.5～7)：(1～10)：(0.1～4.5), more preferably (1～7)：(2～7)：(1～6)：(3～8)：
(0.5～4), most elects (3～5) as：(3～4)：(4～5)：(4～5)：(1～2).In the present invention, the work of the film former
With for promoting exhibition film oil-suspending agent Plastic Flow and elastic deformation, can make exhibition film oil-suspending agent that there is more preferable spreading property.
The balance of oiliness disperse medium of exhibition film oil-suspending agent of the Paecilomyces lilacinus that the present invention is provided.In the present invention, right
The source of the oiliness disperse medium is not particularly limited, using conventional commercial commodity well known to those skilled in the art.
Specific is preferably one or more in white oil, dimethylbenzene, solvent naphtha, methyl naphthalene and ethyl acetate.Oiliness disperse medium
Specifically can be mentioned reagent in any several, can arbitrary proportion mixing.In the present invention, the oiliness disperse medium can
Effective ingredient is uniformly dispersed, makes that exhibition film oil-suspending agent is homogeneous, dispersive property is good.
The present invention does not have special restriction to the preparation method of the exhibition film oil-suspending agent of the Paecilomyces lilacinus, using ability
The preparation method of exhibition film oil-suspending agent known to field technique personnel.Specifically can be by described effective ingredient, surface activity
Prepare after the mixing of agent, film former and oiliness disperse medium.
Present invention also offers Paecilomyces lilacinus exhibition film oil-suspending agent is in terms of preventing and treating crops sucking pest
Application.Paecilomyces lilacinus of the present invention exhibition film oil-suspending agent can prevent and treat each class pest and evil mite, including Meloidogyne incognita,
Cyst roundworm, white ball cyst roundworm, puncture nematicide, hairworm, golden nematode, Margarita nematicide, aphid, wood louse, brown paddy plant hopper etc..
The present invention is not particularly limited to the application method of the exhibition film oil-suspending agent of the Paecilomyces lilacinus, using this area
The application method of exhibition film oil-suspending agent known to technical staff.
Embodiment in below in conjunction with the present invention, to the present invention in technical scheme be clearly and completely described.Aobvious
So, described embodiment is only a part of embodiment of the invention, rather than whole embodiment.Reality in based on the present invention
Example is applied, the every other embodiment obtained under the premise of creative work is not made by those of ordinary skill in the art all belongs to
In the scope of protection of the invention.
Following examples active component content is pressed after hundred kilograms of folding is calculated and is added, and wherein Paecilomyces lilacinus such as nothing is referred in particular to
Going out spore content, spore content is 500,000,000/gram.
Embodiment 1
The preparation of Paecilomyces lilacinus primary seed solution：The Paecilomyces lilacinus strain of preservation is inoculated in and is trained equipped with Nutrient agar
In the eggplant type bottle of foster base, 28 DEG C of culture 12h carry out activation culture, complete actication of culture；Paecilomyces lilacinus strain after activation is pressed
Inoculum concentration according to 1% is inoculated in Paecilomyces lilacinus level liquid culture medium, and level liquid culture medium includes following mass content
Component：Glucose 40g/L, analysis for soybean powder 10g/L, sodium nitrate 10g/L, dipotassium hydrogen phosphate 2.06g/L, potassium chloride 0.5g/L, sulfur
Sour magnesium 0.24g/L, iron sulfate 0.05g/L, balance of water.The temperature of Paecilomyces lilacinus level liquid culture is 28 DEG C, initial pH
It is worth for 6.8, enters in culture 24h according to 15m3/ h ventilations carry out aerobic culture, according to 20m after liquid culture 24h3/ h's is logical
Tolerance carries out aerobic culture, after dark culturing 43h, stops culture, obtains Paecilomyces lilacinus primary seed solution.
The preparation of Paecilomyces lilacinus secondary seed solution：The Paecilomyces lilacinus primary seed solution for obtaining is connect with 10% inoculum concentration
Plant in Paecilomyces lilacinus secondary liquid culture medium, secondary liquid culture medium includes the component of following mass content：Sucrose 40g/
L, analysis for soybean powder 20g/L, sodium nitrate 5g/L, dipotassium hydrogen phosphate 1.5g/L, zinc sulfate 0.24g/L, iron sulfate 0.05g/L are balance of
Water.The temperature of secondary liquid culture is 28 DEG C, and pH value is 6.5, enters in culture 24h, and ventilation is 150m3/ h, cultivates 24h
Afterwards, ventilation is 230m3/ h, is stirred 2 times per hour, and each mixing time is 15min, after dark culturing 65h, stops culture,
Obtain Paecilomyces lilacinus secondary seed solution.
The solid culture of Paecilomyces lilacinus：The Paecilomyces lilacinus secondary seed solution for obtaining is inoculated in 10% inoculum concentration
In Paecilomyces lilacinus solid medium, solid medium includes the component of following mass content：Testa Tritici 50%, Semen Maydis powder 30%,
Analysis for soybean powder 10%, rice husk 9%, ammonium sulfate 1%.The initial water content of Paecilomyces lilacinus solid medium is 50%, solid culture
1d ambient temperatures are 28 DEG C, and temperature of charge is 24 DEG C, and ambient humidity is cultivated under 90%；Solid culture 2d environment temperature
Spend for 24 DEG C, bacterium material temperature degree is 28 DEG C, and ambient humidity is cultivated under 85%, turned over when 30h is cultivated；Solid culture
3d ambient temperatures are 26 DEG C, and temperature of charge is 28 DEG C, and ambient humidity is cultivated under 80%, when 50h is cultivated by 5% Portugal
Grape sugar, 0.002%CaCl2And 0.003%ZnSO4Nutritional solution be sprayed onto on bacterium material；Solid culture 4d ambient temperatures are 26
DEG C, ambient humidity is cultivated under 50%；Solid culture 5d ambient temperatures are 26 DEG C, and ambient humidity is trained under 40%
Support；Solid culture 6d ambient temperatures are 28 DEG C, and ambient humidity is cultivated under field conditions (factors)；Solid culture 7d environment temperature
Spend for 30 DEG C, ambient humidity is cultivated under field conditions (factors), after solid culture 7d, stop culture, the Paecilomyces lilacinus for obtaining
Effectively spore amount is in 500,000,000/more than g.
Bacterium material after by solid culture collects Paecilomyces lilacinus spore using microbe in solid state culture fungal spore separation equipment
The spore of collection is examined under a microscope spore shape by son, to be defined as Paecilomyces lilacinus spore.
Embodiment 2
By weight percentage, the Paecilomyces lilacinus 20% for being prepared by embodiment 1, iso-octyl sodium succinate 7.5%, fat
Polyoxyethylenated alcohol 7.5%, Lauryl Alcohol ester 1%, octadecanol 7%, Propylene Glycol phenylate 6%, dibutyl phthalate 3%,
Monobutyl ether acetate 0.5%, solvent naphtha complements to 100%.
Preparation technology：In the mixing kettle of belt stirrer, first by Paecilomyces lilacinus, iso-octyl sodium succinate, poly alkyl alcohol
Oxygen vinyl Ether, Lauryl Alcohol ester, octadecanol, Propylene Glycol phenylate, dibutyl phthalate, monobutyl ether acetate, solvent naphtha, fully
After stirring mixing, then sand milling in sand mill is pumped into, after sand milling to granular size D90≤5 μm, obtained final product containing Paecilomyces lilacinus exhibition film oil
Suspending agent.
According to preparation technology same as Example 2, following examples are prepared.
Embodiment 3
By weight percentage, the Paecilomyces lilacinus 4% for being prepared by embodiment 1, Affirm (Merck Co.) 2%, iso-octyl
Sodium succinate 7.0%, fatty alcohol-polyoxyethylene ether 7.5%, Lauryl Alcohol ester 7%, octadecanol 3%, Propylene Glycol phenylate 1% are adjacent
Phthalic acid dibutyl ester 3%, monobutyl ether acetate 5%, white oil 22%, solvent naphtha complements to 100%；Obtain containing methylamino AVM hereinafter
The exhibition film oil-suspending agent of rhzomorph and Paecilomyces lilacinus.
Embodiment 4
By weight percentage, the Paecilomyces lilacinus 25% for being prepared by embodiment 1, MTI-446 15%, iso-octyl succinic acid
Sodium 4%, fatty alcohol-polyoxyethylene ether 12%, Lauryl Alcohol ester 2%, octadecanol 2%, Propylene Glycol phenylate 5%, phthalic acid two
Butyl ester 5%, monobutyl ether acetate 2%, ethyl acetate 8%, methyl naphthalene complements to 100%.Obtain blue or green containing MTI-446 and pale purple plan
Mould exhibition film oil-suspending agent..
Embodiment 5
By weight percentage, the Paecilomyces lilacinus 18% for being prepared by embodiment 1, Diacloden 14%, iso-octyl succinic acid
Sodium 8%, fatty alcohol-polyoxyethylene ether 4%, Lauryl Alcohol ester 5%, white oil complements to 100%, obtains containing Diacloden and pale purple
The exhibition film oil-suspending agent of Paecilomyces varioti.
Embodiment 6
By weight percentage, the Paecilomyces lilacinus 26% for being prepared by embodiment 1, avilamycin 4%, iso-octyl succinic acid
Sodium 6.5%, fatty alcohol-polyoxyethylene ether 8.5%, octadecanol 4%, Propylene Glycol phenylate 5%, solvent naphtha complements to 100%.Obtain
Exhibition film oil-suspending agent containing avilamycin and Paecilomyces lilacinus.
Embodiment 7
By weight percentage, the Paecilomyces lilacinus 38% for being prepared by embodiment 1, thiacloprid 12%, iso-octyl succinic acid
Sodium 12%, fatty alcohol-polyoxyethylene ether 8%, monobutyl ether acetate 4%, Lauryl Alcohol ester 7%, white oil complements to 100%, is contained
There is the exhibition film oil-suspending agent of thiacloprid and Paecilomyces lilacinus.
Embodiment 8
By weight percentage, the Paecilomyces lilacinus 4% for being prepared by embodiment 1, flonicamid 15%, iso-octyl succinum
Sour sodium 5%, fatty alcohol-polyoxyethylene ether 14%, Lauryl Alcohol ester 6%, octadecanol 3%, Propylene Glycol phenylate 5%, phthalic acid
Dibutyl ester 4%, monobutyl ether acetate 2%, ethyl acetate complements to 100%, obtains containing flonicamid and Paecilomyces lilacinus
Exhibition film oil-suspending agent.
Embodiment 9
By weight percentage, the Paecilomyces lilacinus 3% for being prepared by embodiment 1, chloromethiuron 45%, iso-octyl sodium succinate
5%, fatty alcohol-polyoxyethylene ether 6%, Lauryl Alcohol ester 2%, octadecanol 4%, Propylene Glycol phenylate 3%, two fourth of phthalic acid
Ester 4%, monobutyl ether acetate 2%, dimethylbenzene complements to 100%, obtains the oil of the exhibition film containing d ichlorbenzuron and Paecilomyces lilacinus and suspends
Agent.
Embodiment 10
By weight percentage, the Paecilomyces lilacinus 18% for being prepared by embodiment 1, Rynaxypyr 22%, iso-octyl
Sodium succinate 6%, fatty alcohol-polyoxyethylene ether 12%, Lauryl Alcohol ester 5%, octadecanol 6%, Propylene Glycol phenylate 4%, adjacent benzene two
Formic acid dibutyl ester 5%, monobutyl ether acetate 1%, solvent naphtha complements to 100%, obtains blue or green containing Rynaxypyr and pale purple plan
Mould exhibition film oil-suspending agent.
Embodiment 11
By weight percentage, the Paecilomyces lilacinus 4% for being prepared by embodiment 1, diflubenzuron 32%, iso-octyl sodium succinate
8%, fatty alcohol-polyoxyethylene ether 8%, Lauryl Alcohol ester 2%, octadecanol 4%, Propylene Glycol phenylate 3%, two fourth of phthalic acid
Ester 4%, monobutyl ether acetate 2%, dimethylbenzene complements to 100%, obtains the oil of the exhibition film containing diflubenzuron and Paecilomyces lilacinus and suspends
Agent.
Embodiment 12
By weight percentage, the Paecilomyces lilacinus 10% for being prepared by embodiment 1, indoxacarb 20%, iso-octyl succinic acid
Sodium 8%, fatty alcohol-polyoxyethylene ether 14%, Lauryl Alcohol ester 4%, octadecanol 2%, Propylene Glycol phenylate 4%, phthalic acid two
Butyl ester 4%, monobutyl ether acetate 1%, white oil complements to 100%, obtains the oil of the exhibition film containing indoxacarb and Paecilomyces lilacinus and suspends
Agent.
Embodiment 13
By weight percentage, the Paecilomyces lilacinus 15% for being prepared by embodiment 1, Affirm (Merck Co.) 1%, thiacloprid
16%, fatty alcohol-polyoxyethylene ether 12%, Lauryl Alcohol ester 2%, octadecanol 2%, Propylene Glycol phenylate 3%, phthalic acid two
Butyl ester 8%, monobutyl ether acetate 1%, solvent naphtha complements to 100%, obtains containing Affirm (Merck Co.), thiacloprid and pale purple plan
The exhibition film oil-suspending agent of penicillium sp.
Embodiment 14
By weight percentage, the Paecilomyces lilacinus 15% for being prepared by embodiment 1, d ichlorbenzuron 12%, indoxacarb 2% are different
Octyl group sodium succinate 8%, Lauryl Alcohol ester 4%, octadecanol 6%, Propylene Glycol phenylate 2%, dibutyl phthalate 4%, fourth
Ether acetate 2%, methyl naphthalene complements to 100%, obtains the exhibition film containing Affirm (Merck Co.), thiacloprid and Paecilomyces lilacinus
Oil-suspending agent.
To embodiment 2～14 obtain the exhibition film oil-suspending agent containing Paecilomyces lilacinus carry out surface capability and to prevent and treat brown
The test of plant hopper effect, with 8% Buprofezin film-spreading oil solution as control, the results are shown in Table 1：
Exhibition film oil-suspending agent surface capability and prevention effect test result of the table 1 containing Paecilomyces lilacinus
As can be drawn from Table 1, adopt present invention offer Paecilomyces lilacinus exhibition film oil-suspending agent sprawl the time for 1.7～
2.2s, spreading rate are 67～73cm/s, and spreading area is 5.7～6.4m2, insecticidal effect is more than 83%.With matched group phase
Than the spreading property of the exhibition film oil-suspending agent of Paecilomyces lilacinus is good, and the insecticidal effect for reaching is stable and is higher than 8% Buprofezin exhibition
Film oil preparation.
As seen from the above embodiment, exhibition film oil-suspending agent of the present invention is compared with traditional film-spreading oil solution, of the invention
The spreading property of the exhibition film oil-suspending agent of offer is good, the effect stability of the pest control for reaching.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. one kind opens up film oil-suspending agent, including the component of following weight/mass percentage composition：
Active component 0.4～50%, described active component include Paecilomyces lilacinus；
Surfactant 3～25%；
Film former 0.6～25%；
The oiliness disperse medium of surplus.
2. exhibition film oil-suspending agent according to claim 1, it is characterised in that spore content living is in the Paecilomyces lilacinus
1～5,000,000,000/gram.
3. according to claim 1 exhibition film oil-suspending agent, it is characterised in that described active component also includes organic insecticidal
Agent, described organic insecticide are Affirm (Merck Co.), MTI-446, Diacloden, avilamycin, thiacloprid, pleocidin, fluorine
Pyridine insect amide, d ichlorbenzuron, Rynaxypyr, diflubenzuron, effective cypermethrin, gamma cyhalothrin, decises and indenes
One or more in worm prestige.
4. exhibition film oil-suspending agent according to claim 3, it is characterised in that the Paecilomyces lilacinus and organic insecticide
Weight ratio is (1～40)：(1～50).
5. according to claim 1 open up film oil-suspending agent, it is characterised in that the film former include Lauryl Alcohol ester, 18
One or more in alcohol, Propylene Glycol phenylate, dibutyl phthalate and monobutyl ether acetate.
6. film oil-suspending agent is opened up according to claim 1 or 5, it is characterised in that the film former is in exhibition film oil-suspending agent
Weight/mass percentage composition be 3～20%.
7. film oil-suspending agent is opened up according to claim 1, it is characterised in that the surfactant includes iso-octyl succinic acid
One or two in sodium and fatty alcohol-polyoxyethylene ether.
8. the exhibition film oil-suspending agent according to claim 1 or 7, it is characterised in that including 5～20% surfactant.
9. according to claim 1 exhibition film oil-suspending agent, it is characterised in that the oiliness disperse medium be white oil, diformazan
One or more in benzene, solvent naphtha, methyl naphthalene and ethyl acetate.
10. the exhibition film oil-suspending agent according to claim 1～9 any one is in preventing and treating crops sucking pest side
The application in face.
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| CN107624772A (en) * | 2017-10-12 | 2018-01-26 | 江西天祥通用航空股份有限公司 | Spiral shell worm ethyl ester opens up film oil-suspending agent |
| CN107624762A (en) * | 2017-10-12 | 2018-01-26 | 江西天祥通用航空股份有限公司 | Bromine cyanogen insect amide opens up film oil-suspending agent |
| CN108576068A (en) * | 2018-06-27 | 2018-09-28 | 河北昊阳化工有限公司 | A kind of mixture composition for preventing below pest and nematode |
| CN110628651A (en) * | 2019-10-21 | 2019-12-31 | 昆明理工大学 | Liquid submerged fermentation method for improving activity of paecilomyces lilacinus spores |
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| CN103004800A (en) * | 2012-12-13 | 2013-04-03 | 西安西诺农化有限责任公司 | Buprofezin spreading oil and preparation method thereof |
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| WO2001080642A1 (en) * | 2000-04-19 | 2001-11-01 | Cognis Deutschland Gmbh | Method for combating insects |
| CN102742607A (en) * | 2012-07-23 | 2012-10-24 | 中国农业大学 | Paecilomyces lilacinus fungal spore oil suspending agent and preparation method as well as application thereof |
| CN103004800A (en) * | 2012-12-13 | 2013-04-03 | 西安西诺农化有限责任公司 | Buprofezin spreading oil and preparation method thereof |
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| CN107624762A (en) * | 2017-10-12 | 2018-01-26 | 江西天祥通用航空股份有限公司 | Bromine cyanogen insect amide opens up film oil-suspending agent |
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Application publication date: 20170315 |