CN107410367A - A kind of paecilomyces fumosoroseus finish and preparation method thereof and a kind of insecticide - Google Patents
A kind of paecilomyces fumosoroseus finish and preparation method thereof and a kind of insecticide Download PDFInfo
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- CN107410367A CN107410367A CN201710778287.4A CN201710778287A CN107410367A CN 107410367 A CN107410367 A CN 107410367A CN 201710778287 A CN201710778287 A CN 201710778287A CN 107410367 A CN107410367 A CN 107410367A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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Abstract
The invention provides a kind of paecilomyces fumosoroseus finish, include the component of following weight/mass percentage composition：Paecilomyces fumosoroseus 30~50%；Solvent 15~40%；Emulsifying agent 2~20%；Adsorbent 2~40%；Ultraviolet absorber 1~10%；Antioxidant 1~10%；Dispersant 2~20%.Paecilomyces fumosoroseus finish prepared by the present invention is housed in 4 DEG C and 25 DEG C of environment respectively, its germination rate was determined every 6 months, the germination rate after 24 months is respectively 90.4% and 82.9%.The Revision insect recluced rate of paecilomyces fumosoroseus finish provided by the invention is 69.1%~86.0%.
Description
Technical field
The invention belongs to pesticide production technology field, more particularly to a kind of paecilomyces fumosoroseus finish and preparation method thereof and
A kind of insecticide.
Background technology
Paecilomyces fumosoroseus (Paecilomycesfumosoroseus) is a kind of wide disease fungus of host range, and it is posted
The main different worm states for including the various insects such as Lepidoptera, Diptera, coleoptera, Hymenoptera, Semiptera.After infecting host, the disease
Fungal pathogenses have pathogenic strong, and the term of validity is longer, and the bacterium shows stronger infecting potential to wide abdomen mantis and a variety of pine moths, has
Stronger killing aphids effect, but the desinsection active principle of paecilomyces fumosoroseus be fungi live spore, its requirement to environment compared with
Height, environment are not suitable for easily causing paecilomyces fumosoroseus natural death failure, cause the time of survival short.
The content of the invention
In view of this, in view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to and provide a kind of paecilomyces fumosoroseus oil
Agent, the time-to-live of paecilomyces fumosoroseus spore can be extended.
The invention provides a kind of paecilomyces fumosoroseus finish, include the component of following weight/mass percentage composition：
Preferably, spore content living is 100~20,000,000,000/gram in the paecilomyces fumosoroseus.
Preferably, the solvent includes the one or more in soybean salad oil, rapeseed oil, peanut oil and animal oil.
Preferably, the emulsifying agent includes fatty acid sorbitan, pungent certain herbaceous plants with big flowers acid glyceride, diacetyl tartarate list diglycerol
One or more in ester, acetylation list diglycerine fatty acid ester and lactic acid fatty glyceride.
Preferably, the adsorbent include diatomite, silica gel, activated alumina, activated carbon, molecular sieve, polyacrylamide,
One or more in silica, vermiculite and calcium silicates.
Preferably, the ultraviolet absorber includes phenylalanine, salicyl ester phenyl ester, ortho-nitraniline, 2,4- dihydroxy two
One or more in Benzophenone, ESCALOL 567 and HMPA.
Preferably, the antioxidant includes dibutyl hydroxy toluene, tert-butylhydroquinone, propylgallate and fourth
One or more in base BHA.
Preferably, the dispersant includes tween, calgon, sodium pyrophosphate, polyacrylic acid sodium salt and polyvinyl alcohol
In one or more.
Present invention also offers the preparation method of described paecilomyces fumosoroseus finish, comprise the following steps：
1) paecilomyces fumosoroseus, adsorbent, ultraviolet absorber and antioxidant are mixed, crushed, obtained containing rose dark brown
The mixture of Paecilomyces varioti；
2) by solvent, emulsifying agent and dispersant, the mixture containing dispersant is obtained；
3) mixture containing paecilomyces fumosoroseus for obtaining the step 1) contains dispersant with what step 2) obtained
Mixture mixing, obtain paecilomyces fumosoroseus finish.
Present invention also offers a kind of insecticide, including described paecilomyces fumosoroseus finish and Nitenpyram, the rose
The mass ratio of dark brown Paecilomyces varioti finish and Nitenpyram is (80~120):(1~20).
The invention provides a kind of paecilomyces fumosoroseus finish, include the component of following weight/mass percentage composition：Rose dark brown is intended
Mould 30~50%；Solvent 15~40%；Emulsifying agent 2~20%；Adsorbent 2~40%；Ultraviolet absorber 1~10%；Antioxygen
Agent 1~10%；Dispersant 2~20%.Paecilomyces fumosoroseus finish prepared by the present invention is housed in 4 DEG C and 25 DEG C of rings respectively
In border, its germination rate was determined every 6 months, the germination rate after 24 months is respectively 90.4% and 82.9%.
Result according to embodiments of the present invention understands that the Revision insect recluced rate of paecilomyces fumosoroseus finish provided by the invention exists
69.1%~86.0%, after being compounded with Nitenpyram, more than 90% was just reached in 3 days to the preventive effect of tea lesser leafhopper after administration,
7 days and 15 days preventive effects are even more to respectively reach 97.9% and 98.8% after dispenser, show that paecilomyces fumosoroseus compounds with Nitenpyram
Agent preventing and treating tea lesser leafhopper has extraordinary effect in deinsectization speed and duration of efficacy.
Biological deposits prove
Paecilomyces fumosoroseus (Paecilomycesfumosoroseus), the micro- life of China was deposited on 08 13rd, 2013
Thing culture presevation administration committee common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese section
Institute of microbiology of institute, biological deposits numbering is CGMCCNo.7993.
Embodiment
The invention provides a kind of paecilomyces fumosoroseus finish, include the component of following weight/mass percentage composition：Rose dark brown is intended
Mould 30~50%；Solvent 15~40%；Emulsifying agent 2~20%；Adsorbent 2~40%；Ultraviolet absorber 1~10%；Antioxygen
Agent 1~10%；Dispersant 2~20%.
Paecilomyces fumosoroseus finish provided by the invention includes the paecilomyces fumosoroseus that weight/mass percentage composition is 30~50%,
Preferably 35~45%, more preferably 40%.
In the present invention, spore content living is preferably 100~20,000,000,000/gram in the paecilomyces fumosoroseus, more preferably
120~18,000,000,000/gram, most preferably 140~16,000,000,000/gram.
In the present invention, the paecilomyces fumosoroseus is preferably selected from the strain that biological deposits numbering is CGMCCNo.7993
Activation culture obtains.
In the present invention, the preparation method of the paecilomyces fumosoroseus comprises the following steps：
1) Paecilomyces Fumosoroseus kind is activated；
2) paecilomyces fumosoroseus after the activation for obtaining the step 1) is inoculated in the training of paecilomyces fumosoroseus level liquid
Support and level liquid culture is carried out in base, obtain paecilomyces fumosoroseus primary seed solution；
3) primary seed solution that the step 2) obtains is inoculated in paecilomyces fumosoroseus secondary liquid culture medium and carried out
Secondary liquid culture, obtain paecilomyces fumosoroseus secondary seed solution；
4) secondary seed solution that the step 3) obtains is inoculated in paecilomyces fumosoroseus solid medium, carries out solid
Culture, obtains paecilomyces fumosoroseus.
In the present invention, the activation of the Paecilomyces Fumosoroseus kind is to be inoculated in the Paecilomyces Fumosoroseus kind of preservation
In eggplant type bottle equipped with nutrient agar, 25~30 DEG C of 10~14h of culture carry out activation culture.
The paecilomyces fumosoroseus of the activation is inoculated into rose dark brown and intends green grass or young crops by the paecilomyces fumosoroseus activated, the present invention
Paecilomyces fumosoroseus level liquid culture is carried out in mould level liquid culture medium, obtains paecilomyces fumosoroseus primary seed solution.
In the present invention, the inoculum concentration of the level liquid culture is preferably 0.5~1.5%, and more preferably 0.8~1.2%, be most preferably
1.0%.
In the present invention, the level liquid culture medium preferably includes the component of following mass content：Dehydrated potato powder 25~
40g/L, 15~25g/L of white granulated sugar, 0.5~1.5g/L of peptone, 0.05~0.15g/L of sodium nitrate, potassium chloride 0.20~
0.30g/L, 0.01~0.08g/L of magnesium sulfate, 0.01~0.08g/L of ferric sulfate, 0.01~0.08g/L of manganese sulfate, phosphoric acid hydrogen two
0.1~0.8g/L of potassium, surplus are water；More preferably：30~35g/L of dehydrated potato powder, 18~22g/L of white granulated sugar, peptone 0.8~
1.2g/L, 0.08~0.12g/L of sodium nitrate, 0.22~0.27g/L of potassium chloride, 0.03~0.07g/L of magnesium sulfate, ferric sulfate 0.03
~0.07g/L, 0.03~0.07g/L of manganese sulfate, 0.3~0.7g/L of dipotassium hydrogen phosphate, surplus are water；Most preferably：Potato
Powder 33g/L, white granulated sugar 20g/L, peptone 1.0g/L, sodium nitrate 0.1g/L, potassium chloride 0.25g/L, magnesium sulfate 0.05g/L, sulphur
Sour iron 0.05g/L, manganese sulfate 0.05g/L, dipotassium hydrogen phosphate 0.5g/L, surplus are water.The present invention does not have to the source of above-mentioned medicine
Have it is specifically limited, using medicine of the those skilled in the art conventionally used for preparing culture medium.
In the present invention, the time of the level liquid culture is preferably 25~40h, more preferably 30~35h, most preferably
For 32h.The temperature of the level liquid culture is preferably 20~30 DEG C, more preferably 23~28 DEG C, most preferably 25 DEG C.It is described
The initial pH value of level liquid culture preferably 6.0~7.0, more preferably 6.4~6.8, most preferably 6.5.
In the present invention, the level liquid culture needs to carry out under agitation, and the frequency of stirring is preferably 1~2 time/h,
More preferably 2 times/h；The time stirred every time is preferably 10~20min, more preferably 12~18min, most preferably 15min.
The present invention does not have special restriction to the equipment of the stirring, using mixing plant well known to those skilled in the art.
In the present invention, ventilated, be particularly preferred as in the process of level liquid culture：Into in culture 24h, ventilation
Measure as 10~20m3/ h, after cultivating 24h, throughput is 15~24m3/h；More preferably：Into in culture 24h, throughput 12
~18m3/ h, after cultivating 24h, throughput is 18~22m3/h；Most preferably：Into in culture 24h, throughput 15m3/ h, training
After supporting 24h, throughput 20m3/h.In the present invention, the air being passed through is particularly preferred as clean, dry, without miscellaneous bacteria
Pure air.The present invention is not particularly limited to the equipment for being passed through pure air, and use is well known to those skilled in the art
Aeration equipment, such as air compressor machine.
In the present invention, the level liquid culture is cultivated under illumination condition.The present invention is to providing illumination condition
Method be not particularly limited, using those skilled in the art routinely select offer illumination condition method.In this hair
In bright, the illumination condition is particularly preferred as using incandescent lighting.
After obtaining paecilomyces fumosoroseus primary seed solution, the present invention connects the one-level paecilomyces fumosoroseus primary seed solution
Kind carries out paecilomyces fumosoroseus secondary liquid culture in paecilomyces fumosoroseus secondary liquid culture medium, obtains paecilomyces fumosoroseus
Secondary seed solution.In the present invention, the inoculum concentration of the secondary liquid culture is preferably 5~15%, and more preferably 8~12%,
Most preferably 10%.
In the present invention, the secondary liquid culture medium preferably includes the component of following mass content：Analysis for soybean powder 20~
30g/L, 20~40g/L of white granulated sugar, 0.05~0.15g/L of sodium nitrate, 5~15g/L of corn flour, 0.1~0.8g/ of dipotassium hydrogen phosphate
L, 0.20~0.30g/L of potassium chloride, 0.01~0.08g/L of ferric sulfate, 0.01~0.08g/L of magnesium sulfate, manganese sulfate 0.01~
0.08g/L, surplus are water；More preferably：23~28g/L of analysis for soybean powder, 25~35g/L of white granulated sugar, 0.08~0.12g/ of sodium nitrate
L, 8~12g/L of corn flour, 0.3~0.7g/L of dipotassium hydrogen phosphate, 0.22~0.28g/L of potassium chloride, 0.03~0.07g/ of ferric sulfate
L, 0.03~0.07g/L of magnesium sulfate, 0.03~0.07g/L of manganese sulfate, surplus are water；Most preferably：Analysis for soybean powder 25g/L, white sand
Sugared 30g/L, sodium nitrate 0.1g/L, corn flour 10g/L, dipotassium hydrogen phosphate 0.5g/L, potassium chloride 0.25g/L, ferric sulfate 0.05g/
L, magnesium sulfate 0.05g/L, manganese sulfate 0.05g/L, surplus are water.The present invention is not particularly limited to the source of above-mentioned medicine, is adopted
With medicine of the those skilled in the art conventionally used for preparing culture medium.
In the present invention, the secondary liquid culture medium preferably uses after 120~130 DEG C sterilize 20~45min.At this
In invention, the sterilising temp is more preferably 121 DEG C；The sterilization time is more preferably 30min.The present invention is to the sterilizing
Equipment is not particularly limited, using sterilizing installation well known to those skilled in the art, such as seeding tank.
In the present invention, the carbon-nitrogen ratio in the paecilomyces fumosoroseus secondary liquid culture medium is suitable, trains level liquid
The strong thalline that foster basal growth goes out produces more paecilomyces fumosoroseus spores, while paecilomyces fumosoroseus spore can be in two level liquid
Sprouted in body culture medium, and then substantial amounts of thalline can be produced in the solid culture stage.
In the present invention, the time of the paecilomyces fumosoroseus secondary liquid culture is preferably 40~56h, and more preferably 45
~50h, most preferably 48h；The temperature of the secondary liquid culture is preferably 20~30 DEG C, more preferably 23~28 DEG C, optimal
Elect 25 DEG C as；The initial pH value of the secondary liquid culture is preferably 6.0~7.0, and more preferably 6.3~6.7, be most preferably
6.5。
In the present invention, air is passed through in secondary liquid incubation, is particularly preferred as entering in culture 24h, throughput
For 130~170m3/ h, after cultivating 24h, throughput is 200~260m3/h；More preferably enter in culture 24h, throughput is
140~160m3/ h, after cultivating 24h, throughput is 210~250m3/h；Most preferably enter in culture 24h, throughput is
150m3/ h, after cultivating 24h, throughput 230m3/h.In the present invention, the air being passed through is particularly preferred as clean, dry
Pure air dry, without miscellaneous bacteria.The present invention is not particularly limited to the equipment for being passed through pure air, using art technology
Aeration equipment known to personnel, such as air compressor machine.
In the present invention, the secondary liquid culture needs to carry out under agitation, and the frequency of stirring is preferably 1~2 time/h,
More preferably 2 times/h；The time stirred every time is preferably 10~20min, more preferably 12~18min, most preferably 15min.
The present invention does not have special restriction to the equipment of the stirring, using mixing plant well known to those skilled in the art.
In the present invention, the secondary liquid culture is cultivated under illumination condition.The present invention is to providing illumination condition
Method be not particularly limited, using those skilled in the art routinely select offer illumination condition method.In this hair
In bright, the illumination condition is particularly preferred as using incandescent lighting.
After obtaining paecilomyces fumosoroseus secondary seed solution, obtained paecilomyces fumosoroseus secondary seed solution is inoculated with by the present invention
In paecilomyces fumosoroseus solid medium, solid culture is carried out, obtains paecilomyces fumosoroseus spore.
In the present invention, the inoculum concentration of the paecilomyces fumosoroseus solid culture is preferably 10~20%, and more preferably 13
~18%, most preferably 15%.
In the present invention, the solid medium preferably includes the component of following mass content：Wheat bran 70~90%, corn
Powder 5~15%, husk 5~15%, ammonium sulfate 0.5~1.5%；More preferably：Wheat bran 75~85%, corn flour 8~12%, paddy
Shell 8~12%, ammonium sulfate 0.8~1.2%；Most preferably：Wheat bran 79%, corn flour 10%, husk 10%, ammonium sulfate 1.0%.
The present invention does not have special restriction to the source of above-mentioned raw materials, and the raw material of culture medium is routinely configured i.e. using those skilled in the art
Can.In the present invention, the initial water content of the paecilomyces fumosoroseus solid medium is preferably 30~70%, and more preferably 40
~60%, most preferably 55%.In the present invention, the culture medium thickness of the solid culture is preferably 1~6cm, and more preferably 2
~4cm, most preferably 3cm.
In the present invention, the control of the process temperature of the paecilomyces fumosoroseus solid culture and humidity, the temperature and
The control program of humidity is particularly preferred as：It is 80~99% in the inner control loop border humidity for entering 1~3d of solid culture, environment temperature
Spend for 24~30 DEG C, bacterium material temperature control is 24~30 DEG C, the inner control loop border humidity for entering 4~5d of solid culture for 40~
50%, environment temperature is 25~30 DEG C, and environment temperature is 28~38 DEG C within 6~7d；More preferably 30~35 DEG C：It is solid entering
1~3d of body culture inner control loop border humidity is 95%, and environment temperature is 28 DEG C, and bacterium material temperature control is 28 DEG C, solid entering
4~5d of body culture inner control loop border humidity be 45%, environment temperature be 28 DEG C, bacterium material temperature degree be natural conditions under temperature, 6~
Environment temperature is 35 DEG C within 7d, and ambient humidity and bacterium material temperature degree are the humidity and temperature under natural conditions.
In the present invention, the paecilomyces fumosoroseus solid culture preferably turns over radiating, more preferably 29 in 28~32h
~31h, most preferably 30h.
In the present invention, nutrient solution is preferably sprayed onto bacterium material by the paecilomyces fumosoroseus solid culture in 45~55h
On, more preferably 48~52h, most preferably 50h.The fountain height of the nutrient solution is preferably the 5~10% of bacterium material quality, more excellent
Elect 6~9%, most preferably 8% as.The present invention is not particularly limited to the equipment for spraying nutrient solution, using people in the art
Spray appliance known to member.In the present invention, the nutrient solution preferably includes the component of following mass content：5% grape
Sugar, 0.001% copper sulphate.The present invention is not particularly limited to the source of above-mentioned medicine, is routinely made using those skilled in the art
Medicine.
After the solid culture, preferred pair of the present invention obtains being collected for conidia powder, obtains paecilomyces fumosoroseus spore.
In embodiments of the present invention, the collection of the conidia powder specifically uses the instrument of microorganism solid fermentation fungal spore separation equipment
Carry out.The instrument of the microorganism solid fermentation fungal spore separation equipment is purchased from Jiangxi Tianren Ecology Co., Ltd..
Paecilomyces fumosoroseus finish provided by the invention includes weight/mass percentage composition for 15~40% solvent, and preferably 20
~35%, more preferably 25~30%.In the present invention, the solvent includes soybean salad oil, rapeseed oil, peanut oil and animal oil
In one or more, it is described it is several be two kinds, three kinds or four kinds.When the solvent is two kinds, three kinds or four kinds, each component
Deng mass mixing.The present invention is not particularly limited to the source of mentioned reagent, the city routinely selected using those skilled in the art
Sell product.In the present invention, the solvent is used for dissolving base-material as a component of finish and prevents gel from increasing
Bin stability.
Paecilomyces fumosoroseus finish provided by the invention includes weight/mass percentage composition for 2~20% emulsifying agent, and preferably 5
~15%, more preferably 8~12%.In the present invention, the emulsifying agent includes fatty acid sorbitan, pungent certain herbaceous plants with big flowers acid glyceride, double
One or more in the single double glyceride of acetyltartaric acid, acetylation list diglycerine fatty acid ester and lactic acid fatty glyceride,
It is described it is several be two kinds, three kinds, four kinds or five kinds.When the emulsifying agent is two kinds, three kinds, four kinds or five kinds, each group is graded
Mass mixing.The present invention is not particularly limited to the source of mentioned reagent, is routinely selected using those skilled in the art commercially available
Product.In the present invention, the emulsifying agent promotes two or more immiscible liquid to form stabilized emulsion.
Paecilomyces fumosoroseus finish provided by the invention includes weight/mass percentage composition for 2~40% adsorbent, and preferably 5
~35%, more preferably 10~30%.In the present invention, the adsorbent include diatomite, silica gel, activated alumina, activated carbon,
One or more in molecular sieve, polyacrylamide, silica, vermiculite and calcium silicates, it is described it is several be two kinds, three kinds or three
More than kind.When the adsorbent is two kinds, three kinds or more when, each group is graded mass mixing.The present invention is to mentioned reagent
Source be not particularly limited, the commercially available prod routinely selected using those skilled in the art.In the present invention, the suction
Attached dose makes active component i.e. paecilomyces fumosoroseus live Spore adhesion in its particle surface, is changed into liquid minor compound additive
Solid compounds, be advantageous to implement uniformly to mix.
Paecilomyces fumosoroseus finish provided by the invention is 1~10% ultraviolet absorber including weight/mass percentage composition, preferably
For 3~8%, more preferably 4~7%.In the present invention, the ultraviolet absorber includes phenylalanine, salicyl ester phenyl ester, adjacent nitre
One kind or several in base aniline, 2,4 dihydroxyl benzophenone, ESCALOL 567 and HMPA
Kind, it is described it is several be two kinds, three kinds, four kinds, five kinds or six kinds.When the ultraviolet absorber is two kinds, three kinds, four kinds, five kinds
Or at six kinds.Each group is graded mass mixing.The present invention is not particularly limited to the source of mentioned reagent, using people in the art
The commercially available prod that member routinely selects.In the present invention, the ultraviolet absorber absorbs the weatherability that ultraviolet improves product
And thermo-oxidative stability.
Paecilomyces fumosoroseus provided by the invention includes weight/mass percentage composition for 1~10% antioxidant, preferably 3~
8%, more preferably 4~7%.In the present invention, the antioxidant include dibutyl hydroxy toluene, tert-butylhydroquinone,
One or more in propylgallate and butylated hydroxy anisole, it is described it is several be two kinds, three kinds or four kinds.When described anti-
When oxidant is two kinds, three kinds or four kinds, each group is graded mass mixing.The present invention does not have special limit to the source of mentioned reagent
It is fixed, the commercially available prod routinely selected using those skilled in the art.In the present invention, the antioxidant improves product
Inoxidizability, extend the service life of product, keep the drug effect of product.
Paecilomyces fumosoroseus finish provided by the invention includes weight/mass percentage composition for 2~20% dispersant, and preferably 5
~15%, more preferably 8~12%.In the present invention, the dispersant includes tween, calgon, sodium pyrophosphate, poly- third
One or more in olefin(e) acid sodium salt and polyvinyl alcohol, it is described it is several be two kinds, three kinds, four kinds or five kinds.When the dispersant
For two kinds, three kinds, four kinds or five kinds when, each group is graded mass mixing.
The preparation method of the paecilomyces fumosoroseus finish obtained present invention also offers such scheme, comprises the following steps：
1) paecilomyces fumosoroseus, adsorbent, ultraviolet absorber and antioxidant are mixed, crushed, obtained containing rose dark brown
The mixture of Paecilomyces varioti；
2) by solvent, emulsifying agent and dispersant, the mixture containing dispersant is obtained；
3) mixture containing paecilomyces fumosoroseus for obtaining the step 1) contains dispersant with what step 2) obtained
Mixture mixing, obtain paecilomyces fumosoroseus finish.
In the present invention, the crushing in the step 1) preferably uses air-flow crushing, the feed pressure of the air-flow crushing
Preferably 0.3~1.2MPa, more preferably 0.5~1.0MPa, most preferably 0.8MPa；The air consumption of the air-flow crushing is excellent
Elect 0.5~1.5m as3/ min, more preferably 0.8~1.3m3/ min, most preferably 1.1m3/min。
In the present invention, the mixture containing paecilomyces fumosoroseus preferably is added to contain and divided by the mixing in the step 3)
In the mixture of powder, the speed of the addition is preferably 0.5~1.5kg/min, and more preferably 0.8~1.2kg/min is optimal
Elect 1kg/min as；The mixing preferably using being mixed by the way of stirring, it is 50 that the speed of the stirring, which is preferably ,~
150r/min, more preferably 80~120r/min, more preferably 100r/min.
In the present invention, the dosage of the paecilomyces fumosoroseus finish is preferably：Paecilomyces fumosoroseus finish is carried out
1000~3000 times of dilutions use, more preferably 1500~2500 times, most preferably 2000 times.The present invention intends the rose dark brown
The occupation mode of mould finish is not particularly limited, and preferably spraying uses.
In the present invention, the paecilomyces fumosoroseus finish is preferably used in mixed way with Nitenpyram, the method used
Preferably Nitenpyram is mixed with water, obtains Nitenpyram solution, then mix with paecilomyces fumosoroseus finish.In the present invention
In, the mass concentration of Nitenpyram is preferably 0.1~2% in the Nitenpyram solution, and more preferably 0.5~1.5%, it is optimal
Elect 1% as；The volume ratio of the Nitenpyram solution and paecilomyces fumosoroseus finish is preferably (1~8):(0.1~2), it is more excellent
Elect as (2~6):(0.5~1.5), most preferably 4:1.
Present invention also offers a kind of insecticide, the insecticide includes the paecilomyces fumosoroseus finish that such scheme obtains
And Nitenpyram, the mass ratio of the paecilomyces fumosoroseus finish and Nitenpyram is preferably (80~120):(1~20), it is more excellent
Elect as (90~110):(5~15), most preferably 100:10.
A kind of paecilomyces fumosoroseus finish provided by the invention and preparation method thereof and one kind are killed with reference to embodiment
Worm agent is described in detail, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
The preparation of paecilomyces fumosoroseus primary seed solution：The Paecilomyces Fumosoroseus kind of preservation is inoculated in equipped with nutrition fine jade
In the eggplant type bottle of fat culture medium, 28 DEG C of culture 12h carry out activation culture, complete actication of culture；Paecilomyces fumosoroseus after activation
Strain is inoculated in paecilomyces fumosoroseus level liquid culture medium according to 1% inoculum concentration, and level liquid culture medium includes following
The component of mass content：Dehydrated potato powder 33g/L, white granulated sugar 20g/L, peptone 1.0g/L, sodium nitrate 0.1g/L, potassium chloride
0.25g/L, magnesium sulfate 0.05g/L, ferric sulfate 0.05g/L, manganese sulfate 0.05g/L, dipotassium hydrogen phosphate 0.5g/L, surplus are water.
The temperature of paecilomyces fumosoroseus level liquid culture be 25 DEG C, initial pH value 6.5, into culture 24h according to 15m3/ h leads to
Tolerance carries out ventilation culture, according to 20m after Liquid Culture 24h3/ h throughput carries out ventilation culture, after illumination cultivation 32h, stops
Only cultivate, obtain paecilomyces fumosoroseus primary seed solution.
The preparation of paecilomyces fumosoroseus secondary seed solution：By obtained paecilomyces fumosoroseus primary seed solution with 10% inoculation
Amount is inoculated in paecilomyces fumosoroseus secondary liquid culture medium, and secondary liquid culture medium includes the component of following mass content：It is yellow
Bean powder 25g/L, white granulated sugar 30g/L, sodium nitrate 0.1g/L, corn flour 10g/L, dipotassium hydrogen phosphate 0.5g/L, potassium chloride 0.25g/
L, ferric sulfate 0.05g/L, magnesium sulfate 0.05g/L, manganese sulfate 0.05g/L, surplus are water.The temperature of secondary liquid culture is 25
DEG C, pH value 6.5, into culture 24h, throughput 150m3/ h, after cultivating 24h, throughput 230m3/ h, is stirred per hour
Mix 2 times, each mixing time is 15min, after illumination cultivation 48h, stops culture, obtains paecilomyces fumosoroseus secondary seed solution.
The solid culture of paecilomyces fumosoroseus：Obtained paecilomyces fumosoroseus secondary seed solution is connect with 15% inoculum concentration
For kind in paecilomyces fumosoroseus solid medium, solid medium includes the component of following mass content：Wheat bran 79%, corn flour
10%, husk 10%, ammonium sulfate 1.0%.The initial water content of paecilomyces fumosoroseus solid medium is 55%, solid culture the
1d environment temperatures are 25 DEG C, and bacterium material temperature degree is 25 DEG C, and ambient humidity is cultivated under 95%；Solid culture 2d environment temperatures
For 28 DEG C, bacterium material temperature degree is 28 DEG C, and ambient humidity is cultivated under 95%, is turned over when cultivating 30h；Solid culture
3d environment temperatures are 28 DEG C, and bacterium material temperature degree is 28 DEG C, and ambient humidity is cultivated under 80%, when cultivating 50h by 5% grape
Sugar, the nutrient solution of 0.001% copper sulphate are sprayed onto on bacterium material, and the fountain height of nutrient solution is the 5% of bacterium material quality；Solid culture
4d environment temperatures are 28 DEG C, and ambient humidity is cultivated under 50%, and bacterium material temperature degree is the temperature under natural environment；Solid culture
5d environment temperatures are 28 DEG C, and ambient humidity is cultivated under 40%, and bacterium material temperature degree is the temperature under natural environment；Solid is trained
It is 30 DEG C to support 6d environment temperatures, and ambient humidity and bacterium material temperature degree are the humidity and temperature under natural conditions；Solid culture 7d
Environment temperature is 35 DEG C, and ambient humidity and bacterium material temperature degree are the humidity and temperature under natural conditions, after solid culture 7d, stops training
Support, the obtained effective spore amount of paecilomyces fumosoroseus 200 spores/gram.
Bacterium material after solid culture is collected into paecilomyces fumosoroseus using microbe in solid state culture fungal spore separation equipment
Spore, the spore of collection is observed into spore shape under the microscope, to be defined as paecilomyces fumosoroseus spore.
Embodiment 2
The preparation of paecilomyces fumosoroseus finish：It is 30% by mass percent in terms of the total amount of paecilomyces fumosoroseus finish
The paecilomyces fumosoroseus that is obtained in embodiment 1,10% diatomite, 10% silica gel, 2% phenylalanine, 2% salicyl ester phenyl ester, 3%
Dibutyl hydroxy toluene, the mixing of 3% tert-butylhydroquinone, crush, obtain the mixture containing paecilomyces fumosoroseus；By 20%
Soybean salad oil, 5% fatty acid sorbitan, 5% pungent certain herbaceous plants with big flowers acid glyceride, 5% calgon, the mixing of 5% sodium pyrophosphate, are obtained
Mixture containing dispersant；Mixture containing paecilomyces fumosoroseus is mixed with the mixture containing dispersant, obtains rose
Dark brown Paecilomyces varioti finish.
Embodiment 3
The preparation of paecilomyces fumosoroseus finish：It is 35% by mass percent in terms of the total amount of paecilomyces fumosoroseus finish
The paecilomyces fumosoroseus that is obtained in embodiment 1,15% activated alumina, 3% ortho-nitraniline, 3%2,4- dihydroxy hexichol first
Ketone, 2% tert-butylhydroquinone, the mixing of 2% propylgallate, crushing, obtain the mixture containing paecilomyces fumosoroseus；Will
20% rapeseed oil, 5% pungent certain herbaceous plants with big flowers acid glyceride, 5% diacetyl tartarate list double glyceride, 5% tween, 5% calgon mix
Close, obtain the mixture containing dispersant；Mixture containing paecilomyces fumosoroseus is mixed with the mixture containing dispersant,
Obtain paecilomyces fumosoroseus finish.
Embodiment 4
The preparation of paecilomyces fumosoroseus finish：It is 40% by mass percent in terms of the total amount of paecilomyces fumosoroseus finish
Paecilomyces fumosoroseus, 10% molecular sieve, 10% polyacrylamide, 4% hexamethyl phosphinylidyne, the 6% butyl hydroxyl obtained in embodiment 1
The mixing of base anisole, crush, obtain the mixture containing paecilomyces fumosoroseus；By 20% peanut oil, 5% acetylation list diglycerol
Fatty acid ester, the mixing of 5% polyacrylic acid sodium salt, obtain the mixture containing dispersant；By the mixing containing paecilomyces fumosoroseus
Thing mixes with the mixture containing dispersant, obtains paecilomyces fumosoroseus finish.
Embodiment 5
The preparation of paecilomyces fumosoroseus finish：It is 45% by mass percent in terms of the total amount of paecilomyces fumosoroseus finish
The paecilomyces fumosoroseus that is obtained in embodiment 1,5% polyacrylamide, 5% silica, 5% vermiculite, 3% phenylalanine, 3%
Ortho-nitraniline, the mixing of 4% dibutyl hydroxy toluene, crush, obtain the mixture containing paecilomyces fumosoroseus；By 20% bone
Oil, 5% lactic acid fatty glyceride, 5% polyvinyl alcohol, obtain the mixture containing dispersant；Rose dark brown will be contained to intend
The mixture of mould mixes with the mixture containing dispersant, obtains paecilomyces fumosoroseus finish.
Embodiment 6
The preparation of paecilomyces fumosoroseus finish：It is 50% by mass percent in terms of the total amount of paecilomyces fumosoroseus finish
The paecilomyces fumosoroseus spore that is obtained in embodiment 1,10% calcium silicates, 4% salicyl ester phenyl ester, 3% tert-butylhydroquinone,
The mixing of 3% propylgallate, crush, obtain the mixture containing paecilomyces fumosoroseus；By 10% soybean salad oil, 10% dish
Seed oil, the mixing of 5% fatty acid sorbitan, 5% tween, obtain the mixture containing dispersant；Paecilomyces fumosoroseus will be contained
Mixture mixes with the mixture containing dispersant, obtains paecilomyces fumosoroseus finish.
Embodiment 7
Paecilomyces fumosoroseus work spore prepared by embodiment 1 and paecilomyces fumosoroseus finish prepared by embodiment 4 are distinguished
It is housed in 4 DEG C and 25 DEG C of environment, its germination rate was determined every 6 months, the results are shown in Table 1.
The paecilomyces fumosoroseus finish of table 1 and paecilomyces fumosoroseus work spore store germination rate in 2 years
As can be drawn from Table 1, the storage of paecilomyces fumosoroseus finish lives spore than pure paecilomyces fumosoroseus work spore in two years
Sub- survival benefit is good.
Embodiment 8
The test of pesticide effectiveness：This experiment is carried out in Jiangxi Province, and preventing and treating kind is cotton, and management is good, and bollworm occurs medium
Lay particular stress on.Experimental field is divided into six regions by this experiment, and region 1 is control group, a spray clear water；Region 2 sprays embodiment 2
2000 times of dilutions of paecilomyces fumosoroseus finish of middle preparation；Region 3 sprays the paecilomyces fumosoroseus finish prepared in embodiment 3
2000 times of dilutions；Region 4 sprays the 2000 times of dilutions of paecilomyces fumosoroseus finish prepared in embodiment 4；Region 5 sprays reality
Apply the 2000 times of dilutions of paecilomyces fumosoroseus finish prepared in example 5；Region 6 sprays the rose dark brown prepared in embodiment 6 and intends green grass or young crops
Mould 2000 times of dilutions of finish；The dosage in six regions is identical, respectively investigates borer population of once living within 3 days, 7 days and 15 days after dispenser,
Calculate Revision insect recluced rate.It the results are shown in Table 2.
Desinsection (bollworm) effect of the paecilomyces fumosoroseus finish of table 2
It can be drawn by the data of table 2, except control group region, the Revision insect recluced rate in other five regions is all higher,
All reach more than 80%.Show that the insecticidal effect of paecilomyces fumosoroseus finish prepared by the present invention is relatively good, especially, four, five,
Six regions are high compared with the Revision insect recluced rate in other regions, and comprehensive real economy benefit show that the paecilomyces fumosoroseus spore of addition is kept
It is best in mass percent 40%.Meanwhile after dispenser 15 days, each dispenser region all maintains the high Revision insect recluced rate of comparison, table
Paecilomyces fumosoroseus finish prepared by the bright present invention has the preferable lasting property of medicine.
Embodiment 9
The preparation of paecilomyces fumosoroseus and Nitenpyram built agent：Nitenpyram is mixed with water, it is molten to obtain Nitenpyram
Liquid so that the mass concentration of Nitenpyram is 1% in Nitenpyram solution, then the rose cigarette that will be prepared in this solution and embodiment 4
Color Paecilomyces varioti finish is with volume ratio 4:1 mixing, obtains paecilomyces fumosoroseus and Nitenpyram built agent.
Embodiment 10
Built agent Toxicity Determination：Make test plant with cucumber, Potted orchard is put into insect cage, and access Bemisia tabaci
Adult, drives its whole out of after it is laid eggs one day, and 26 ± 1 DEG C of temperature and 14 is kept in cage:10 illumination, treats cucumber leaves
On Bemisia tabaci when being developed to 2 age nymph, virulence test is carried out using infusion process, 3 repetitions, investigates the effect of 10 days after medicine,
Corrected mortality is calculated, asks the regression equation of the death rate niqueMin of drug concentration logarithm one, LC50, calculated altogether with reference to the abundant methods of Sun Yun
Malicious coefficient (CTC), CTC ＜ 100 are antagonism, and CTC is summation action close to 100, and CTC ＞ 100 are synergistic effect, and it is tested
As a result it is as shown in table 3.
Toxicity Determination of the built agent of table 3 to Bemisia tabaci
Note：Ratio is volume ratio in table
As can be seen from Table 3, paecilomyces fumosoroseus and the co-toxicity coefficient of each ratio of Nitenpyram built agent are all greater than 100
, show that paecilomyces fumosoroseus finish and Nitenpyram compounding are synergistic effects, especially, when paecilomyces fumosoroseus finish with
The volume ratio of Nitenpyram solution is 1:Synergistic effect is most obvious when 4, corresponding with the result in embodiment 8, illustrates this knot
Fruit is rational.
Embodiment 11
Field test of the built agent to tea lesser leafhopper：This experiment is carried out in Jiangxi Province, and tea shoot grows fine, the tea
Fertile soil, sandy loam, micelle grain, level land, fertility level is higher, pH value 6.9, is covered in tea ground without weeds, sunny, should
Ground is the Chang Faqu of tea lesser leafhopper, and tea tree breed is to meet frost.The ground is divided into four regions by this experiment, and region 1 is pair
According to group, a spray clear water；Region 2 sprays the 2000 times of dilutions of paecilomyces fumosoroseus finish prepared in embodiment 4；Spray in region 3
Apply 4% Nitenpyram solution, 2000 times of dilutions；Region 4 sprays the 2000 times of dilutions of built agent prepared in embodiment 9；Four
The dosage in region is identical, respectively investigates borer population of once living within 1 day, 3 days, 7 days and 15 days after dispenser, calculates Revision insect recluced rate and prevent
Effect.It the results are shown in Table 4.
Field test of the built agent of table 4 to tea lesser leafhopper
It can be drawn by the data of table 4, the preventive effect that paecilomyces fumosoroseus ULV Eliminating tea lesser leafhopper is used alone is being applied
Reach up to 73.3% within 3 days after medicine, and preventive effect gradually reduces over time；Nitenpyram solution is used alone to prevent
The preventive effect for controlling tea lesser leafhopper respectively reaches 92.2% and 94.8% in 7 days and 15 days after administration, illustrates that Nitenpyram solution is prevented
Control that the duration of efficacy of tea lesser leafhopper is longer, and effect is also preferable；Use paecilomyces fumosoroseus prepared by the present invention and alkene pyridine
The preventive effect of worm amine built agent preventing and treating tea lesser leafhopper just reaches more than 90% in 3 days after administration, and 7 days and 15 days preventive effects be more after dispenser
It is to respectively reach 97.9% and 98.8%, shows that paecilomyces fumosoroseus prevents and treats tea lesser leafhopper in deinsectization with Nitenpyram built agent
There is extraordinary effect in speed and duration of efficacy.
It can be seen from above example, the Revision insect recluced rate of paecilomyces fumosoroseus finish provided by the invention 69.1%~
86.0%, after being compounded with Nitenpyram, more than 90% was just reached in 3 days to the preventive effect of tea lesser leafhopper after administration, 7 days after dispenser
It is even more to respectively reach 97.9% and 98.8% with 15 days preventive effects, shows that paecilomyces fumosoroseus and Nitenpyram built agent preventing and treating tea are small
Greenery cicada has extraordinary effect in deinsectization speed and duration of efficacy.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of paecilomyces fumosoroseus finish, include the component of following weight/mass percentage composition：
2. paecilomyces fumosoroseus finish according to claim 1, it is characterised in that：Spore living in the paecilomyces fumosoroseus
Content is 100~20,000,000,000/gram.
3. paecilomyces fumosoroseus finish according to claim 1, it is characterised in that：The solvent include soybean salad oil,
One or more in rapeseed oil, peanut oil and animal oil.
4. paecilomyces fumosoroseus finish according to claim 1, it is characterised in that：The emulsifying agent includes aliphatic acid sorb
Smooth, pungent certain herbaceous plants with big flowers acid glyceride, diacetyl tartarate list double glyceride, acetylation list diglycerine fatty acid ester and lactic acid fatty acid glycerine
One or more in ester.
5. paecilomyces fumosoroseus finish according to claim 1, it is characterised in that：The adsorbent includes diatomite, silicon
One or more in glue, activated alumina, activated carbon, molecular sieve, polyacrylamide, silica, vermiculite and calcium silicates.
6. paecilomyces fumosoroseus finish according to claim 1, it is characterised in that：The ultraviolet absorber includes phenylpropyl alcohol ammonia
Acid, salicyl ester phenyl ester, ortho-nitraniline, 2,4 dihydroxyl benzophenone, ESCALOL 567 and hempa
One or more in acyl triamine.
7. paecilomyces fumosoroseus finish according to claim 1, it is characterised in that：The antioxidant includes dibutyl hydroxyl
One or more in base toluene, tert-butylhydroquinone, propylgallate and butylated hydroxy anisole.
8. paecilomyces fumosoroseus finish according to claim 1, it is characterised in that：The dispersant includes tween, six inclined
One or more in sodium phosphate, sodium pyrophosphate, polyacrylic acid sodium salt and polyvinyl alcohol.
9. the preparation method of the paecilomyces fumosoroseus finish described in claim 1~8 any one, comprises the following steps：
1) paecilomyces fumosoroseus, adsorbent, ultraviolet absorber and antioxidant are mixed, crushed, obtain intending green grass or young crops containing rose dark brown
Mould mixture；
2) by solvent, emulsifying agent and dispersant, the mixture containing dispersant is obtained；
3) mixture containing paecilomyces fumosoroseus for obtaining the step 1) obtains mixed containing dispersant with step 2)
Compound mixes, and obtains paecilomyces fumosoroseus finish；
There is no time sequencing restriction between the step 1) and step 2).
10. a kind of insecticide, including paecilomyces fumosoroseus finish described in claim 1~8 any one or claim 9 are made
Standby obtained paecilomyces fumosoroseus finish, Nitenpyram, the mass ratio of the paecilomyces fumosoroseus finish and Nitenpyram is (80
~120):(1~20).
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