CN106416756A - Cordyceps militaris illumination intermittent culture technology - Google Patents
Cordyceps militaris illumination intermittent culture technology Download PDFInfo
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- CN106416756A CN106416756A CN201611077540.5A CN201611077540A CN106416756A CN 106416756 A CN106416756 A CN 106416756A CN 201611077540 A CN201611077540 A CN 201611077540A CN 106416756 A CN106416756 A CN 106416756A
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- illumination
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
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Abstract
The invention discloses a cordyceps militaris illumination intermittent culture technology. The technology comprises the following steps of sterilization, inoculation, hyphae period culture, mycelium culture, sporocarp culture and harvesting; during sporocarp culture, 4-5 small holes are punctured in a plastic film, the temperature is controlled to be 22 DEG C, the relative air humidity is kept to be 80%, 50W incandescent lamps are used for performing illumination for 16 h per day and turned off for one hour every four hours, after sporocarp heads are started to expand and are not lengthened any more, illumination is performed for 18 hours per day, and the lamps are turned off for one hour every six hours, the temperature is kept to be 23 DEG C, and the relative air humidity is kept to be 90%. In this way, according to the cordyceps militaris illumination intermittent culture technology, in the mycelium culture later period and the sporocarp culture process, intermittent illumination at different periods of time is adopted, the adverse effect caused by continuous illumination on hyphae and sporocarp is avoided, the growth speed is higher, and the cordyceps militaris yield is higher.
Description
Technical field
The present invention relates to cultivating technique is interrupted in Cordyceps militaris (L.) Link. field, more particularly to a kind of Cordyceps militaris (L.) Link. illumination.
Background technology
Cordyceps militaris (L.) Link. is a kind of ascomycetess, carries out syngenesis by heterothallism.Its phorozoon is Cordyceps militaris (L.) Link. Paecilomyces varioti.Its son
Ascospore can be formed after entity maturation（Reproduction unit）, spore propagated after distributing with the wind, and spore falls on suitable polypide, just
Start sprouting and form mycelium.Mycelium one side is constantly developed, and simultaneously starts to spread into polypide, and then pupa worm will be true
Bacterium infects, and decomposes the tissue in pupal cell, is originated using the nutrition in pupal cell as the matter and energy of its growth promoter, finally by pupa
Internal portion decomposes completely.
Natural Cordyceps militaris (L.) Link. yields poorly, complicated component, and the Cordyceps militaris (L.) Link. yield of artificial culture is high, and composition is relatively pure.But
It is that lasting illumination is unfavorable for the growth of Cordyceps militaris (L.) Link. individuality during cultivation, needs to control illumination, improve cultivating technique.
Content of the invention
The present invention solves the technical problem of providing a kind of Cordyceps militaris (L.) Link. illumination to be interrupted cultivating technique, implement discontinuous photograph
Bright, reduce the unfavorable factor to Cordyceps militaris (L.) Link. individual growth.
For solving above-mentioned technical problem, one aspect of the present invention is：A kind of Cordyceps militaris (L.) Link. illumination interruption is provided
Cultivating technique, comprises the following steps：
Sterilizing：Vial culture medium is carried out sterilization, 110 DEG C of high temperature sterilize half an hour in pressure cooker is placed on, is then dropped to
Take out after room temperature, move to inoculation indoor；
Inoculation：Several living silkworm chrysalises are put into into vial culture medium, liquid spawn is then rapidly injected, then with transparent modeling
Material thin film moves into culturing room after sealing bottleneck；
Bacteria developing period culture：Sending out the indoor dark of bacterium initial stage holding culture and ventilation is being avoided, relative air humidity is remaining 68%, controls
Temperature processed is 17 DEG C, rises high-temperature to 20 DEG C after charge level covers with mycelia；
Mycelia culture：Mycelia culture early stage is 2 ~ 3 days, and early stage is cultivated indoor temperature and be controlled to 22 DEG C daytime, night temperatures be
DEG C, relative air humidity 65%, illumination is carried out using 45 watts of electric filament lamp, light application time is 10 hours；
The mycelia culture later stage is 3 ~ 6 days, and day temperature is that 25 DEG C, night temperatures are 10 DEG C, and relative air humidity is maintained at 85%,
And illumination is carried out using 45 watts of electric filament lamp, light application time is that 16 hours, illumination was turned off the light half an hour every 4 hours；
Mycelia gradually becomes crocus by white, is further cultured for 2 days after switching to crocus completely entering to go out the careless phase for 2 days, and culture is indoor
Temperature is maintained at 24 DEG C, air relative temperature 90%, and 50 watts of electric filament lamp carries out the illumination of daily 18 hours, and illumination was every 6 hours
Turn off the light 1 hour；
Sporophore culture：4 ~ 5 apertures are pierced on a plastic film, temperature control is at 22 DEG C, and relative air humidity is maintained at 80%,
50 watts of electric filament lamp carries out the illumination of daily 16 hours, and illumination was turned off the light 1 hour every 4 hours, treated that sporophore head starts to expand
And when no longer elongated, the illumination of daily 18 hours being changed to, illumination was turned off the light 1 hour every 6 hours, and temperature is kept for 23 DEG C, air phase
90% is kept to humidity；
Harvest：After sporophore head expand holding constant after, be further cultured for 4 ~ 6 days so that apical head produce ascospore when, table
Show Cordyceps militaris (L.) Link. maturation, culturing room is removed, take out Cordyceps militaris (L.) Link..
In a preferred embodiment of the present invention, in the supply line of the electric filament lamp, time switch is provided with.
In a preferred embodiment of the present invention, the quantity of the living silkworm chrysalises is 3 ~ 5, spaces between at least
For 2cm.
In a preferred embodiment of the present invention, the injection rate of the liquid spawn is 10ml.
The invention has the beneficial effects as follows：A kind of Cordyceps militaris (L.) Link. illumination interruption cultivating technique that the present invention is pointed out, in mycelia culture
In later stage and sporophore incubation, be respectively adopted the compartment illumination of different time, it is to avoid continuous light to mycelia and
The harmful effect of sporophore, faster, the yields of Cordyceps militaris (L.) Link. is higher for the speed of growth.
Specific embodiment
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described enforcement
Example is only a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, this area is common
All other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model of present invention protection
Enclose.
The embodiment of the present invention includes：
A kind of Cordyceps militaris (L.) Link. illumination is interrupted cultivating technique, including：Comprise the following steps：
Sterilizing：Vial culture medium is carried out sterilization, 110 DEG C of high temperature sterilize half an hour in pressure cooker is placed on, is then dropped to
Take out after room temperature, move to inoculation indoor；
Inoculation：Several living silkworm chrysalises are put into into vial culture medium, and the quantity of the living silkworm chrysalises is 3 ~ 5, each other
Interval be at least 2cm, be then rapidly injected 10ml liquid spawn, then with transparent plastic sheeting seal after bottleneck move into training
Foster room；
Bacteria developing period culture：Sending out the indoor dark of bacterium initial stage holding culture and ventilation is being avoided, relative air humidity is remaining 68%, controls
Temperature processed is 17 DEG C, rises high-temperature to 20 DEG C after charge level covers with mycelia；
Mycelia culture：Mycelia culture early stage is 2 ~ 3 days, and early stage is cultivated indoor temperature and be controlled to 22 DEG C daytime, night temperatures be
DEG C, relative air humidity 65%, illumination is carried out using 45 watts of electric filament lamp, light application time is 10 hours, keeps electric filament lamp in glass
The surface of bottle culture medium, reduces the oblique illumination to vial culture medium；
The mycelia culture later stage is 3 ~ 6 days, and day temperature is that 25 DEG C, night temperatures are 10 DEG C, and relative air humidity is maintained at 85%,
And illumination is carried out using 45 watts of electric filament lamp, light application time is that 16 hours, illumination was turned off the light half an hour every 4 hours；
Mycelia gradually becomes crocus by white, is further cultured for 2 days after switching to crocus completely entering to go out the careless phase for 2 days, and culture is indoor
Temperature is maintained at 24 DEG C, air relative temperature 90%, and 50 watts of electric filament lamp carries out the illumination of daily 18 hours, and illumination was every 6 hours
Turn off the light 1 hour, in the supply line of the electric filament lamp, be provided with time switch, lifting operation convenience and light interval control essence
Degree；
Sporophore culture：4 ~ 5 apertures are pierced on a plastic film, temperature control is at 22 DEG C, and relative air humidity is maintained at 80%,
50 watts of electric filament lamp carries out the illumination of daily 16 hours, and illumination was turned off the light 1 hour every 4 hours, treated that sporophore head starts to expand
And when no longer elongated, the illumination of daily 18 hours being changed to, illumination was turned off the light 1 hour every 6 hours, and temperature is kept for 23 DEG C, air phase
90% is kept to humidity；
Harvest：After sporophore head expand holding constant after, be further cultured for 4 ~ 6 days so that apical head produce ascospore when, table
Show Cordyceps militaris (L.) Link. maturation, culturing room is removed, take out Cordyceps militaris (L.) Link..
In sum, a kind of Cordyceps militaris (L.) Link. illumination interruption cultivating technique that the present invention is pointed out, using the phototaxis of sporophore, adopts
With the discontinuous illumination being positioned above, sporophore is more sturdy, reduces bending of the sporophore straight up in growth course, carries
High length and yield
Embodiments of the invention are the foregoing is only, the scope of the claims of the present invention is not thereby limited, every utilization present invention says
Equivalent structure or equivalent flow conversion that bright book content is made, or other related technical fields are directly or indirectly used in, all
It is included within the scope of the present invention in the same manner.
Claims (4)
1. a kind of Cordyceps militaris (L.) Link. illumination is interrupted cultivating technique, it is characterised in that comprise the following steps：
Sterilizing：Vial culture medium is carried out sterilization, 110 DEG C of high temperature sterilize half an hour in pressure cooker is placed on, is then dropped to
Take out after room temperature, move to inoculation indoor；
Inoculation：Several living silkworm chrysalises are put into into vial culture medium, liquid spawn is then rapidly injected, then with transparent modeling
Material thin film moves into culturing room after sealing bottleneck；
Bacteria developing period culture：Sending out the indoor dark of bacterium initial stage holding culture and ventilation is being avoided, relative air humidity is remaining 68%, controls
Temperature processed is 17 DEG C, rises high-temperature to 20 DEG C after charge level covers with mycelia；
Mycelia culture：Mycelia culture early stage is 2 ~ 3 days, and early stage is cultivated indoor temperature and be controlled to 22 DEG C daytime, night temperatures be
DEG C, relative air humidity 65%, illumination is carried out using 45 watts of electric filament lamp, light application time is 10 hours；
The mycelia culture later stage is 3 ~ 6 days, and day temperature is that 25 DEG C, night temperatures are 10 DEG C, and relative air humidity is maintained at 85%,
And illumination is carried out using 45 watts of electric filament lamp, light application time is that 16 hours, illumination was turned off the light half an hour every 4 hours；
Mycelia gradually becomes crocus by white, is further cultured for 2 days after switching to crocus completely entering to go out the careless phase for 2 days, and culture is indoor
Temperature is maintained at 24 DEG C, air relative temperature 90%, and 50 watts of electric filament lamp carries out the illumination of daily 18 hours, and illumination was every 6 hours
Turn off the light 1 hour；
Sporophore culture：4 ~ 5 apertures are pierced on a plastic film, temperature control is at 22 DEG C, and relative air humidity is maintained at 80%,
50 watts of electric filament lamp carries out the illumination of daily 16 hours, and illumination was turned off the light 1 hour every 4 hours, treated that sporophore head starts to expand
And when no longer elongated, the illumination of daily 18 hours being changed to, illumination was turned off the light 1 hour every 6 hours, and temperature is kept for 23 DEG C, air phase
90% is kept to humidity；
Harvest：After sporophore head expand holding constant after, be further cultured for 4 ~ 6 days so that apical head produce ascospore when, table
Show Cordyceps militaris (L.) Link. maturation, culturing room is removed, take out Cordyceps militaris (L.) Link..
2. Cordyceps militaris (L.) Link. illumination according to claim 1 is interrupted cultivating technique, it is characterised in that the supply lines of the electric filament lamp
Time switch is provided with road.
3. Cordyceps militaris (L.) Link. illumination according to claim 1 is interrupted cultivating technique, it is characterised in that the quantity of the living silkworm chrysalises
For 3 ~ 5, at least 2cm is spaced between.
4. Cordyceps militaris (L.) Link. illumination according to claim 1 is interrupted cultivating technique, it is characterised in that the injection of the liquid spawn
Measure as 10ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611077540.5A CN106416756A (en) | 2016-11-30 | 2016-11-30 | Cordyceps militaris illumination intermittent culture technology |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611077540.5A CN106416756A (en) | 2016-11-30 | 2016-11-30 | Cordyceps militaris illumination intermittent culture technology |
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| CN106416756A true CN106416756A (en) | 2017-02-22 |
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ID=58222444
Family Applications (1)
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| CN201611077540.5A Pending CN106416756A (en) | 2016-11-30 | 2016-11-30 | Cordyceps militaris illumination intermittent culture technology |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108707527A (en) * | 2018-03-16 | 2018-10-26 | 蒋丽霞 | High conidia powder cordyceps wine enjoys assembly production method and its culture assembly |
| CN108925365A (en) * | 2018-06-22 | 2018-12-04 | 江苏省农业科学院 | A kind of Chinese caterpillar fungus culture medium and the Cordyceps militaris inoculation method based on the culture medium |
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| CN102668884A (en) * | 2012-05-30 | 2012-09-19 | 无锡诺达克生物照明科技有限公司 | Method for promoting growth of edible mushrooms by utilizing LED (Light-Emitting Diode) light source |
| CN103283478A (en) * | 2012-02-24 | 2013-09-11 | 北京市弘科农场 | Cordyceps militaris producing method |
| CN104303820A (en) * | 2014-09-09 | 2015-01-28 | 山东健方生物科技有限公司 | Large-scale production method of living silkworm cordyceps militaris |
| CN105532263A (en) * | 2015-12-23 | 2016-05-04 | 遵义鸿霖生物技术有限公司 | Cultivation method for Cordyceps militaris through alternate illumination with blue and white lights of LED |
-
2016
- 2016-11-30 CN CN201611077540.5A patent/CN106416756A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283478A (en) * | 2012-02-24 | 2013-09-11 | 北京市弘科农场 | Cordyceps militaris producing method |
| CN102668884A (en) * | 2012-05-30 | 2012-09-19 | 无锡诺达克生物照明科技有限公司 | Method for promoting growth of edible mushrooms by utilizing LED (Light-Emitting Diode) light source |
| CN104303820A (en) * | 2014-09-09 | 2015-01-28 | 山东健方生物科技有限公司 | Large-scale production method of living silkworm cordyceps militaris |
| CN105532263A (en) * | 2015-12-23 | 2016-05-04 | 遵义鸿霖生物技术有限公司 | Cultivation method for Cordyceps militaris through alternate illumination with blue and white lights of LED |
Non-Patent Citations (1)
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| 孟繁宇: "蛹虫草高产菌株人工栽培条件的优化", 《食用菌》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108707527A (en) * | 2018-03-16 | 2018-10-26 | 蒋丽霞 | High conidia powder cordyceps wine enjoys assembly production method and its culture assembly |
| CN108925365A (en) * | 2018-06-22 | 2018-12-04 | 江苏省农业科学院 | A kind of Chinese caterpillar fungus culture medium and the Cordyceps militaris inoculation method based on the culture medium |
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Application publication date: 20170222 |