A kind of chemical luminescent detecting system
Technical field
The present invention relates to a kind of chemical luminescent detecting systems.
Background technology
Existing chemical luminescent detecting system although automatic detection can be realized, there are problems, for example detects not
Enough accurate, structure is not compact enough, and occupied space is big, also unreasonable there are structure design, be easy to cause reactant and is contaminated, separately
Outside, existing automation equipment degree is not high enough, therefore, it is necessary to design a kind of more perfect chemical luminescent detecting system of function
System.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of chemical luminescent detecting system, the chemical luminescent detecting system
Compact-sized, operation is smooth, easy to implement.
The technical solution of invention is as follows:
A kind of chemical luminescent detecting system, including pedestal, rotating platform, liquid taking device, cleaning device, fluid injection/mixing dress
It puts, incubating device, reading plotter and lose cup device;
Rotating platform is arranged on the center of pedestal, liquid taking device, cleaning device, fluid injection/evenly mixing device, incubating device, reading
It counting apparatus and loses cup device and is arranged on around rotating platform on pedestal;
Rotating platform:Rotating platform includes the stepper motor and gear of stent, turntable, arc carrier and driving disc;
Turntable is arranged on stent, and turntable is endless member, and the inner ring side of turntable is equipped with what is be adapted to the gear
Double wedge;Gear is mounted in the shaft of stepper motor;
Arc carrier is fixed on by arc carrier vertical columns on turntable, can be rotated synchronously with turntable;On arc carrier
Equipped with for accommodating the fixture of reaction cup;
Arc carrier is equipped with the magnet fixed block with magnet;The first Hall sensor is fixed on stent, first suddenly
Your sensor is concordant with the height of magnet.
Stent is equipped with to detect the reaction cup detection sensor that reaction cup whether there is.
The Hall sensor is multiple, along being arranged circumferentially on stent for turntable.
The stent includes support ring and is used to support more support columns of support ring.
It supports column bottom and is equipped with firm banking.
Reaction cup rotating mechanism is also known as rotating platform, and for reaction cup to be driven to switch in each module, which has
The arc carrier of internal-gear type rotary turnplate mechanism, placing response cup;
The arc carrier of the placing response cup is fixed on turntable, and for fixture for clamping reaction cup, instrument is (i.e. chemical
Luminescence assays system) operation when, manually reaction cup place arc carrier on, clamp reaction cup ensures doing circumference fortune
Reaction cup is not rocked when dynamic;
The Hall sensor is used to detect the position that reaction cup reaches each station;The reaction cup detection sensing
Device is used to detect whether arc carrier has reaction cup.
The rotating platform is putting cup position, is taking liquid level, liquid blending position, is incubated position, cleaning position, reading position and loses cup position difference
Hall sensor is provided with, for sensing the position that reaction cup reaches each station, magnet follows rotary turnplate in the work of motor
It is circled under, when turntable rotation to corresponding position, magnet senses with Hall sensor, and turntable stops, will be at this
Position carries out corresponding detection operation.
Fluid injection/evenly mixing device:Including the second riser, cam horizontal sliding mechanism and for the mechanism for filling liquid for reaction cup fluid injection;
Cam horizontal sliding mechanism includes the second cam, the second actuated by cams motor for driving cam rotation, pushing block, second
Guide rod, second back-moving spring;
The mechanism for filling liquid includes liquid injection pipe fixed plate and the liquid injection pipe being vertically fixed in liquid injection pipe fixed plate;
Riser is equipped with pilot hole, and guide rod is inserted into pilot hole;Liquid injection pipe fixed plate and pushing block, which are separately positioned on, leads
To the front end and rear end portion of bar;Resetting spring is sleeved on guide rod;
Second cam is contacted with pushing block;When second cam rotates, the second cam can drive liquid injection pipe fixed plate and liquid injection pipe
It travels forward.
Second guide rod is 2, and the pilot hole is 2.Fluid injection/evenly mixing device further includes detection machine in place
Structure, it is described to position detecting mechanism include the static inducting component that is arranged on riser and being arranged on push away it is movable on cup mechanism
Trigger unit.Movable trigger unit and static inducting component are respectively magnetic sheet and the second Hall sensor, alternatively, the work
Dynamic formula trigger unit and static inducting component are respectively light barrier and photoelectricity to penetrating detection module.
Baffle is additionally provided on second riser, baffle is located at the lower section of liquid injection pipe fixed plate, and baffle, which is equipped with, to be used for as fluid injection
The bar shaped guide hole of pipe guided in translation;
Fluid injection/evenly mixing device includes rabbling mechanism, and rabbling mechanism includes rabbling mechanism fixed plate, stirring motor and for covering
The firmly sleeve of reaction cup.
The rabbling mechanism is driven by Z axis driving mechanism.
Incubating device:Incubating device includes vertical plate and the incubation block being arranged on vertical plate;The outer wall for being incubated block is equipped with
Thermal insulation board;It is incubated in block and is equipped with heating unit;
The incubation block is connected by Z axis straight-line motion mechanism with vertical plate.
Z axis straight-line motion mechanism is lead screw transmission mechanism, including the 3rd motor, screw, screw link block and is vertically directed
Bar;
Upper plate and lower plate, the top and bottom for being vertically directed bar are respectively equipped at the top and bottom of vertical plate
It is separately fixed on upper plate and lower plate;Screw link block is mounted on and is vertically directed on bar, and driving motor is fixed on upper plate
On;The upper end of screw connects the 3rd motor, the lower end connection wire rod link block of leading screw;Block is incubated to be fixed on screw link block;
When 3rd motor rotates, driving is incubated block edge and is vertically directed bar elevating movement.Bar is vertically directed as 2.
Reading plotter:
Reading plotter includes bearing, lift drive mechanism, fluid injection/drain needle assemblies, darkroom and PMT reading modules;
The bearing is equipped with the guide rail of vertical direction;Lift drive mechanism includes the 4th motor, transmission mechanism and leads
Rail link block (95);Guide rail link block is mounted on guide rail, and PMT reading modules are fixed on guide rail link block, and darkroom is read with PMT
Digital-to-analogue block is connected;Motor drives PMT readings module and darkroom to make elevating movement along guide rail by transmission mechanism and guide rail link block;
Fluid injection/drain needle assemblies are mounted on bearing, and darkroom is located at the underface of fluid injection/drain needle assemblies;During detection, treat
The reaction cup of detection is located in darkroom.
Transmission mechanism is lead screw transmission mechanism.4th motor is stepper motor.Z-axis transmission machine is equipped on bearing
Structure, fluid injection/drain needle assemblies are mounted on by Z-axis transmission mechanism on bearing.
Lose cup device:Losing cup device includes the first riser, cam mechanism and pushes away cup mechanism;
Cam mechanism includes the first cam and the first actuated by cams motor for driving cam rotation;
The cup mechanism that pushes away includes push plate, the first guide rod, (the first back-moving spring and wedge-shaped pushing block;
Riser is equipped with the pilot hole for being used for pushing away cup mechanism guiding, and the first guide rod is inserted into pilot hole;Wedge shape pushes away
Block and push plate are separately positioned on the front end and rear end portion of the first guide rod;Resetting spring is sleeved on the first guide rod;
First cam is contacted with push plate;When first cam rotates, the first cam, which can drive, to be pushed away cup mechanism and travels forward, wedge shape
When pushing block advances, the fixture of clamping reaction cup can be pushed open so that reaction cup departs from fixture.
First guide rod is 2, and the pilot hole is 2.
It loses cup device and further includes Diu Bei testing agencies, the Diu Bei testing agencies include the static sense being arranged on riser
It answers component and is arranged on the movable trigger unit pushed away on cup mechanism;Movable trigger unit and static inducting component are respectively magnetic
Piece and Hall sensor, alternatively, the movable trigger unit and static inducting component is respectively light barrier and photoelectricity to penetrating
Detection module.
Detection process comprises the following steps:
Step 1:Placing response cup;
The turntable of rotating platform is in initial position, i.e. zero-bit (A), the fixture being at this time placed on reaction cup on arc panel
On;
Step 2:Take liquid step;
Turntable rotation causes reaction cup arrival to take liquid level (B), and reaction cup is injected liquid by Qu Ye mechanisms;The liquid
Including reagent and sample;The completion of Qu Ye mechanisms takes liquid and fluid injection is existing mature technology
Step 3:First time mixing step;
Turntable rotation causes reaction cup to reach mixing position (C), and the rabbling mechanism of fluid injection/evenly mixing device is to the liquid in reaction cup
Body is stirred;
Step 4:Incubation step
Turntable rotation causes reaction cup reaches to be incubated position (D), carries out heating to the liquid in reaction cup by incubating device and incubates
Educate operation;
Step 5:Cleaning step;
Turntable rotation causes reaction cup to reach cleaning position (E), using the magnetic bead in wiper mechanism cleaning reaction cup;
Step 6:Second of mixing step;
Turntable rotation causes reaction cup to reach mixing position, and the rabbling mechanism injection triggering agent A liquid of fluid injection/evenly mixing device is simultaneously
Liquid in reaction cup is stirred;
Step 7:Reading step;
Turntable rotation causes reaction cup to reach reading position (F), and injection triggering agent B liquid, reading plotter reads reaction result;
Take waste liquid after reading away;
Step 8:Lose a glass step;
Turntable rotation causes reaction cup arrival to lose cup position (G), pushes away by reaction cup by losing cup device and (into hopper and receive
Collection);
It completes once for the determination step of reaction cup liquid.
After step 8, turntable continues to be rotated back to zero-bit, chemical luminescent detecting system reset.
Triggering agent A and triggering agent B are also known as trgA and trgB, and ingredient is respectively sodium hydroxide and hydrogen peroxide, plays and urges
Change acts on.
PMT is photomultiplier, is a staple product in photon technology device, it is a kind of with high sensitivity
With the light-detecting device of ultrafast time response.It can be widely applied to photon counting, atomic weak light detection, chemiluminescence, biology hair
Light research, the detection of extremely low energy-ray, spectrophotometer, polarimeter, colorimeter, illumination photometer, konimeter, nephelometer, optical density
In the instrument and equipments such as meter, thermoluminescence amount instrument, radio heat meter, scanning electron microscope, Biochemical Analyzer.
Advantageous effect:
Rotating platform is arranged on middle part by the chemical luminescent detecting system of the present invention, other mechanisms are set around rotating platform
It puts, reasonable structural arrangement;
It (is measured moreover, the system is provided with liquid taking device, cleaning device, fluid injection/mixing (stirring) device, reading plotter
Device) and cup device is lost, it can realize the whole flow process of luminescence assays, only need manually by reaction cup as on rotating platform, just
It can realize complicated testing process, export measurement result, high degree of automation.
Where the advantage of each crucial submodule of following division:
(1) cup device is lost automatically, and pushing away cup mechanism realization using the first cam mechanism loses cup operation, compact-sized, knot
Structure is ingenious, and operation is smooth, can be with rotating platform slitless connection, tight fit action.
(2) reaction cup rotating mechanism integrates driving mechanism, testing agency (detection reaction cup whether there is), detent mechanism
It is compact-sized into an entirety, position detection is carried out using Hall sensor cooperation magnet, there is accurate positioning, adopt
With gear and headgear, by driving stepper motor, have the advantages that operation is smooth.
(3) fluid injection/evenly mixing device promotes mechanism for filling liquid to implement fluid injection using cam mechanism, immediately exits from noting after the completion of fluid injection
Liquid level avoids reactant from being contaminated;In short, mechanism is compact-sized, operation is smooth, can be with detection device slitless connection.
(4) incubating device can ensure that incubating device is transported using lead-screw drive mechanism and the guiding mechanism based on upright guide rail
Row is steady and smooth, is incubated on block and sets thermal insulation board, can effectively prevent heat and scatter and disappear, and is equipped with multiple holes in addition, being incubated in block, energy
Incubation operation is carried out to the sample in multiple reaction cups simultaneously, it is efficient.
(5) interpretation device (i.e. reading plotter) is in the light using darkroom, the accuracy of reading is ensured, using based on stepping
The elevating mechanism of motor, lead screw transmission mechanism and guide rail can ensure fluid injection/smooth operation of drain needle assemblies, using two sets of transmissions
Mechanism controls PMT modules and fluid injection/drain needle assemblies lifting respectively, can neatly control fluid injection, drain and detection, therefore, this
Kind interpretation device has the advantages that compact-sized, perfect in shape and function, reading accuracy is high.
In conclusion the chemical luminescent detecting system function of the present invention is enriched, each several part operation is smooth, can cooperate with work mutually
Make, structure design is compact and reasonable, common to cooperate, and can complete complicated measure flow, high degree of automation.
Description of the drawings
Fig. 1 is the overall layout chart of chemical luminescent detecting system;
Fig. 2 is the stereogram of chemical luminescent detecting system;
Fig. 3 is luminescence assays flow chart;
Fig. 4 is the automatic general structure schematic diagram (stereogram) for losing cup device of chemical luminescent detecting system;
Fig. 5 is the enlarged drawing of A1 parts in Fig. 1;
Fig. 6 is the automatic general structure schematic diagram (top view) for losing cup device of chemical luminescent detecting system.
Fig. 7 is the general structure schematic diagram (stereogram) of rotating platform;
Fig. 8 is the general structure schematic diagram (top view) of rotating platform;
Fig. 9 is the general structure schematic diagram (side view) of rotating platform.
Figure 10 is the structure diagram (stereogram) of incubating device;
Figure 11 is the structure diagram (top view) of incubating device;
Figure 12 is reading plotter structure diagram.
Label declaration:
46- firm bankings, 47- arc carrier vertical columns, 48- turntables, 49- support rings, 50- arc carriers, 521-
One Hall sensor, 531- reaction cup detection sensors, 541- support columns, 551- magnet fixed blocks, 561- gears, 571- steppings
Motor, 581- motor fixed racks.The first risers of 51-, 52- the first actuated by cams motors, the first cams of 53-, 54- push plates, 55-
(the first back-moving spring, 56- wedge shape pushing blocks, 57- fixtures, the first guide rods of 58-, 59- hoppers, 60- light barriers, 61- photoelectricity is to penetrating
Detection module.
The 3rd motors of 62-, 63- upper plates, 64- vertical plates, 65- screw link blocks, 66- are vertically directed bar, 67- bottoms
Plate, 68- screws, 69- are incubated block, 70- thermal insulation boards.
The second risers of 71-, 72- baffles, 73- liquid injection pipe fixed plates, 74- liquid injection pipes, 75- magnetic sheets, the second hall sensings of 76-
Device, 77- second back-moving springs, the second cams of 78-, 79- the second actuated by cams motors, the second guide rods of 80-, 81- stirring motors,
82- sleeves, 83- rabbling mechanism fixed plates.
The 4th motors of 89-, 90- motor link blocks;91- is in the light column, 92- darkrooms, 93-PMT modules, 94- guide rails, 95-
Guide rail link block, 105- fluid injections/drain needle assemblies.
A- zero-bits, B- take liquid level, C- mixings position, and D- is incubated position, E- cleaning positions, F- readings position, and G- loses cup position.
Specific embodiment
The present invention is described in further details below with reference to the drawings and specific embodiments:
Embodiment 1:
Such as Fig. 1-12, a kind of chemical luminescent detecting system, including pedestal, rotating platform, liquid taking device, cleaning device, note
Liquid/evenly mixing device, incubating device, reading plotter and lose cup device;
Rotating platform is arranged on the center of pedestal, liquid taking device, cleaning device, fluid injection/evenly mixing device, incubating device, reading
It counting apparatus and loses cup device and is arranged on around rotating platform on pedestal;
Rotating platform is putting cup position, is taking liquid level, liquid blending position, is incubated position, cleaning position, reading position and loses cup position and set respectively
Hall sensor is equipped with, for sensing the position that reaction cup reaches each station, magnet follows effect of the rotary turnplate in motor
Under circle, when turntable rotation to corresponding position, magnet senses with Hall sensor, turntable stop, will be in the position
Put the corresponding detection operation of progress.
Losing cup device includes the first riser 51, the first cam mechanism and pushes away cup mechanism;
First cam mechanism includes the first cam 53 and the first actuated by cams motor 52 for driving the rotation of the first cam;
The cup mechanism that pushes away includes push plate 54, the first guide rod 58, (the first back-moving spring 55 and wedge-shaped pushing block 56:
First riser is equipped with the pilot hole for being used for pushing away cup mechanism guiding, and the first guide rod is inserted into pilot hole;Wedge
Shape pushing block and push plate are separately positioned on the front end and rear end portion of the first guide rod;(the first back-moving spring is sleeved on the first guide rod
On;
First cam is contacted with push plate;When first cam rotates, the first cam, which can drive, to be pushed away cup mechanism and travels forward, wedge shape
When pushing block advances, the fixture 57 of clamping reaction cup can be pushed open so that reaction cup departs from fixture, and cup is lost in realization.
First guide rod is 2, and the pilot hole is 2.
Automatic cup device of losing further includes Diu Bei testing agencies, and the Diu Bei testing agencies include being arranged on the first riser
Static inducting component and be arranged on the movable trigger unit pushed away on cup mechanism.
Movable trigger unit and static inducting component are respectively magnetic sheet and Hall sensor, alternatively, described is movable
Trigger unit and static inducting component are respectively light barrier 60 and photoelectricity to penetrating detection module 61.
Automatic cup device of losing further includes rotating platform for installs fixture, and the fixture is multiple, mounted on rotation
On platform.
Lose a glass process:It loses before a glass process starts, loses cup device and be in original state, wedge-shaped pushing block resets bullet first at this time
In retraction position, the initial position of the wedge-shaped pushing block in retraction position position under the action of spring;
A glass process is lost to comprise the following steps:
Step 1:Alignment procedures:The wedge-shaped pushing block for pushing away cup mechanism alignment is clamped with to the fixture of reaction cup;
Step 2:Push away a glass step:Starting the first actuated by cams motor, the first actuated by cams motor drives the rotation of the first cam,
First cam promotes push plate to advance, and wedge-shaped pushing block links with push plate, and wedge-shaped pushing block pushes fixture arrival open and pushes away cup position so that reaction cup
Depart from the clamping of fixture, cup is lost in completion;
Step 3:Reset process:The rear half-turn that first actuated by cams motor shaft rotates a circle acts for the first cam return,
Wedge-shaped pushing block returns to initial position under the action of (the first back-moving spring, and the sensing chip being fixed at this time on pushing block is passed with optocoupler
Sensor senses, motor stalls;Cup is lost to terminate;Wait Orders initiation is waited to push away a glass step next time;
The position of wedge-shaped pushing block is detected using Diu Bei testing agencies;
When wedge-shaped pushing block is in initial position, the static inducting component that is arranged on the first riser, which can sense, to be arranged on
Push away the movable trigger unit on cup mechanism;
When wedge-shaped pushing block, which is in, pushes away glass position, the static inducting component cannot sense movable trigger unit.
Movable trigger unit and static inducting component are respectively magnetic sheet and Hall sensor, alternatively, described is movable
Trigger unit and static inducting component are respectively light barrier 60 and photoelectricity to penetrating detection module 61.
Fixture to be multiple, be arranged on rotating platform and along rotating platform circumferentially, by controlling rotary flat
The rotation angle of platform causes wedge-shaped pushing block and grip alignment.
Hopper 59 is additionally provided with below wedge-shaped pushing block, the reaction cup for that will abandon is oriented to collecting box.
Rotating platform includes stent, turntable 48, arc carrier 50 and the stepper motor 571 of driving disc and gear 561;
Turntable is arranged on stent, and turntable is endless member, and the inner ring side of turntable is equipped with what is be adapted to the gear
Double wedge;Gear is mounted in the shaft of stepper motor;
Arc carrier is fixed on by arc carrier vertical columns on turntable, can be rotated synchronously with turntable;On arc carrier
Equipped with for accommodating the fixture 57 of reaction cup;
Arc carrier is equipped with the magnet fixed block 551 with magnet;The first Hall sensor 521 is fixed on stent,
Hall sensor is concordant with the height of magnet.
Stent is equipped with to detect the reaction cup detection sensor 531 that reaction cup whether there is.
The Hall sensor is multiple, along being arranged circumferentially on stent for turntable.
The stent includes support ring and is used to support more support columns 541 of support ring.
It supports column bottom and is equipped with firm banking 46.
Reaction cup rotating mechanism is also known as rotating platform, and for reaction cup to be driven to switch in each module, which has
The arc carrier of internal-gear type rotary turnplate mechanism, placing response cup;
The arc carrier of the placing response cup is fixed on turntable, and for fixture for clamping reaction cup, instrument is (i.e. chemical
Luminescence assays system) operation when, manually reaction cup place arc carrier on, clamp reaction cup ensures doing circumference fortune
Reaction cup is not rocked when dynamic;
The Hall sensor is used to detect the position that reaction cup reaches each station;The reaction cup detection sensing
Device is used to detect whether arc carrier has reaction cup.
Fluid injection/evenly mixing device (evenly mixing device is also known as agitating device) includes the second riser 71, cam horizontal sliding mechanism and is used for
For fluid injection/evenly mixing device of reaction cup fluid injection;
Cam horizontal sliding mechanism include the second cam 78, for driving cam rotation the second actuated by cams motor 79, pushing block,
Second guide rod 80, second back-moving spring 77;
Fluid injection/the evenly mixing device includes liquid injection pipe fixed plate 73 and the fluid injection being vertically fixed in liquid injection pipe fixed plate
Pipe 74;
Second riser is equipped with pilot hole, and guide rod is inserted into pilot hole;Liquid injection pipe fixed plate and pushing block are set respectively
In the front end and rear end portion of the second guide rod;Resetting spring is sleeved on the second guide rod;
Second cam is contacted with pushing block;When second cam rotates, the second cam can drive liquid injection pipe fixed plate and liquid injection pipe
It travels forward, when reaching front end, starts fluid injection, after the completion of fluid injection, liquid injection pipe fixed plate and liquid injection pipe retract reset.
Second guide rod is 2, and the pilot hole is 2.
Fluid injection/evenly mixing device of the chemical luminescent detecting system further includes position detecting mechanism, the inspection in place
Surveying mechanism includes the static inducting component being arranged on riser and is arranged on the movable trigger unit pushed away on cup mechanism.
Movable trigger unit and static inducting component are respectively 75 and second Hall sensor 76 of magnetic sheet, alternatively, described
Movable trigger unit and static inducting component be respectively light barrier and photoelectricity to penetrating detection module.
Baffle 72 is additionally provided on riser, baffle is located at the lower section of liquid injection pipe fixed plate, and baffle, which is equipped with, to be used for as liquid injection pipe
The bar shaped guide hole of guided in translation.
Fluid injection/evenly mixing device of chemical luminescent detecting system further includes the rabbling mechanism below baffle, rabbling mechanism
Including rabbling mechanism fixed plate 83, stirring motor 81 and sleeve 82 for entangling reaction cup.
The rabbling mechanism is driven by Z axis driving mechanism.
Rear stirrer structure moves upwards reaction cup in place, and reaction cup is pressed into stirring sleeve under the stop of baffle, needs at this time
Will be to reaction cup fluid injection, the second actuated by cams motor action, driving fluid injection/evenly mixing device advances, in place after, noted for reaction cup
Liquid.After the completion of fluid injection, the second actuated by cams motor does not reoperate, and under the action of resetting spring, fluid injection/evenly mixing device resets.
After liquid liquid enters, stirring motor, which starts, drives stirring sleeve to carry out high-speed motion, to achieve the effect that liquid blending.
Incubating device includes vertical plate 64 and the incubation block 69 being arranged on vertical plate;The outer wall for being incubated block is equipped with thermal insulation board
70;It is incubated in block and is equipped with heating unit.
The incubation block is connected by Z axis straight-line motion mechanism with vertical plate.
Z axis straight-line motion mechanism is lead screw transmission mechanism, including the 3rd motor 62, screw 68, screw link block 65 and is erected
Straight guide rod 66;
Upper plate 63 and lower plate 67 are respectively equipped at the top and bottom of vertical plate, is vertically directed bar as 2 so that be incubated
Block operation is more steady.
The top and bottom for being vertically directed bar are separately fixed on upper plate and lower plate;Screw link block is mounted on vertical
On guide rod, the 3rd motor is fixed on upper plate;The upper end connection driving motor of screw, the lower end connection wire rod connection of leading screw
Block;Block is incubated to be fixed on screw link block;When 3rd motor rotates, driving is incubated block edge and is vertically directed bar elevating movement.
When needing to be incubated the sample in reaction cup, incubation block is risen to come, after the completion of incubation, block will be incubated
Lower resigning.It is incubated and multiple holes for being used to accommodate reaction cup is equipped at the top of block.
Reading plotter includes bearing, lift drive mechanism, fluid injection/drain needle assemblies 105, darkroom 92 and PMT reading modules
93;
The bearing is equipped with the guide rail 94 of vertical direction;Lift drive mechanism includes the 4th motor 89, transmission mechanism
With guide rail link block 95;Guide rail link block is mounted on guide rail, and PMT reading modules are fixed on guide rail link block, darkroom and PMT
Reading module is connected;4th motor drives PMT readings module and darkroom to be risen along guide rail by transmission mechanism and guide rail link block
Drop movement;
Fluid injection/drain needle assemblies are mounted on bearing, and darkroom is located at the underface of fluid injection/drain needle assemblies;During detection, treat
The reaction cup of detection is located in darkroom, and the column 91 that is in the light is used to be in the light;
Z-axis transmission mechanism is equipped on bearing, fluid injection/drain needle assemblies are mounted on by Z-axis transmission mechanism on bearing.
Transmission mechanism is lead screw transmission mechanism.
4th motor is stepper motor.Motor link block 90 is used to coordinate with leading screw.During detection, reaction cup is placed in
In darkroom, the liquid in reaction cup is detected PMT modules and reading.Fluid injection/drain needle assemblies add reaction cup after declining
Liquid and drain operation.PMT modules are docked with darkroom, and the two is located at sustained height (i.e. positioned at the same horizontal position).
When reactant reaches the system, fluid injection/drain needle assemblies decline the injection substrate in reaction cup and generate signal, PMT
Reading module, which rises to close up again, carries out signal detection and output data, and in the process, light-baffling device (darkroom) provides for system
Dark room conditions.
Darkroom module is fixed in PMT reading modules, and PMT readings module is used to carry out reading detection, darkroom to reactant
The ambient of module isolation reactant ensures the accuracy of reading;Fluid injection/drain needle assemblies include liquid injection pipe fixed plate, fluid injection
Pipe, drain pipe, influidic pipe fixed block, which is used to generate signal to reactant injection substrate, after drain pipe is used for reading
Reaction cup carries out waste liquid exclusion;Fluid injection realizes accurate liquid feeding by plunger pump, and plunger pump is a kind of energy liquid with precise control sample-adding
The device of amount;The component is existing mature modules.