CN106386467A - Efficient stamen killing method for japonica rice - Google Patents
Efficient stamen killing method for japonica rice Download PDFInfo
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- CN106386467A CN106386467A CN201610886258.5A CN201610886258A CN106386467A CN 106386467 A CN106386467 A CN 106386467A CN 201610886258 A CN201610886258 A CN 201610886258A CN 106386467 A CN106386467 A CN 106386467A
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- stamen
- killing
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- rice
- gametocide
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to an efficient stamen killing method for japonica rice. The method includes: digging out female parents which need hybridization in the afternoon of the day before a blooming day or in the morning of the blooming day, shearing off excessively old and excessively tender tillers, selecting rice spikes which enter full blooming in 2-3 days, shearing open glumes along the middles of anthers with scissors, removing bloomed glumous flowers during glume shearing, and upwardly watering from the roots of the rice spikes with clean water to complete stamen killing; sleeving the female parents after the stamen killing with kraft bags, moving the female parents to places near male parents, and taking the male parents for pollination during full blooming. The clean water stamen killing method for the japonica rice has the advantages that convenient and fast stamen killing of the japonica rice is achieved, the method is low in glume shearing requirements and thorough in stamen killing, and hybridization efficiency is increased evidently; the problems that a hot-water stamen killing method kills pistils due to excessively high temperature and is not thorough in stamen killing due to excessively low temperature are solved, and the method is low in pistil damage, good in pollination effect, high in hybrid outcrossing seed-setting, and the like.
Description
Technical field
The invention belongs to the gametocide technical field during japonica rice cross-breeding, more particularly, to a kind of japonica rice of efficient quick
Gametocide method.
Background technology
During Oryza sativa L. scientific research breeding, when especially Rice Heading is bloomed, only more than the ten day time at florescence, the time is short,
The not only heading of intermediate materials to be recorded, observes Hua Shi etc., also wants Selection parent, carries out hybridizing combo, workload is very huge,
This time is again very crucial for whole breeding work --- the basis of Subsequent product breeding technique, hybridization combo kind work
Amount maximum should be the work of artificial gametocide, and this work is key of hybridization success or failure again, if can use during this period a kind of good
With simplest technological means, and good gametocidal effect can be obtained again, for scientific worker, can not only effectively subtract
The workload of light breeding scientific research personnel, additionally it is possible to improve crossing work efficiency well, reaches the effect got twice the result with half the effort.
Content of the invention
During the present invention is directed to current rice cultivating, artificial gametocide workload is big, and ineffective problem provides one
Rapidly and efficiently efficiently gametocide method in kind of japonica rice crossover process.
For solving above-mentioned technical problem, the technical solution used in the present invention comprises the following steps；
(1) bloom noon before that day by or morning on the same day need to do the maternal band soil hybridizing and dig out, cut off old and excessively tender
Tiller；
(2) pollination noon before that day or morning on the same day, choose the spike of rice in two or three days entering full blossom, with shears by clever shell edge
Flower pesticide middle and lower part breaks (flower pesticide must break), during cutting grain husk, rejects the grain husk flower opened, then uses clear water from fringe base
Watering upwards to fringe top in portion, that is, completes gametocide；
(3) female parent after gametocide is put kraft paper bag, the maternal concentration putting kraft paper bag is planted together；
(4) take male parent to pollinate one by one during full blossom, then the maternal kraft paper bag fold closure after pollination made regular check on,
Perform insect protected, mould proof work, cut receipts when cenospecies are ripe.
The present invention is to contain starch using in pollen grain using the cardinal principle of such scheme, pollen grain water suction after watering,
Pollen grain is burst, so that pollen is lost vigor, there is after gametocide following technique effect：
Achieve japonica rice gametocide convenient, fast, clever technical requirements are low to cutting, hybridizing rate more than 99%, gametocide is more thorough, miscellaneous
Hand over efficiency significantly improve, gynoecium is killed because temperature is too high compared with lukewarm water gametocide, or too low cause gametocide not thorough, to gynoecium damage
Little, Pollination Effect is good, the advantages of hybrid outcrossing is high.
Specific embodiment
Further illustrate the present invention with reference to embodiment, but not limited to this.
Embodiment 1
(1) bloom noon before that day by or morning on the same day need to do the maternal band soil hybridizing and dig out, cut off old and excessively tender
Tiller；
(2) pollination noon before that day or morning on the same day, choose the spike of rice in two or three days entering full blossom, with shears by clever shell edge
Flower pesticide middle and lower part breaks (flower pesticide must break), during cutting grain husk, rejects the grain husk flower opened, then uses clear water from fringe base
Watering upwards to fringe top in portion, that is, completes gametocide；
(3) female parent after gametocide is put kraft paper bag, the maternal concentration putting kraft paper bag is planted together；
(4) take male parent to pollinate one by one during full blossom, then the maternal kraft paper bag fold closure after pollination made regular check on,
Perform insect protected, mould proof work, cut receipts when cenospecies are ripe.
With the desirable embodiment of the present invention for enlightenment, by above-mentioned description, relevant staff completely can be
In the range of this invention technological thought, carry out various change and modification.The technical scope of this invention is simultaneously
The content being not limited in description it is necessary to determine its technical scope according to right.
Claims (1)
1. a kind of efficient japonica rice gametocide method is it is characterised in that the method comprises the following steps：
(1) bloom noon before that day by or morning on the same day need to do the maternal band soil hybridizing and dig out, cut off old and excessively tender point
Tiller；
(2) pollination noon before that day or morning on the same day, choose the spike of rice in two or three days entering full blossom, with shears by clever shell along flower pesticide
Middle and lower part breaks, and during cutting grain husk, rejects the grain husk flower opened, is then watered upwards to fringe top from fringe base portion with clear water, that is,
Complete gametocide；
(3) female parent after gametocide is put kraft paper bag, the maternal concentration putting kraft paper bag is planted together；
(4) take male parent to pollinate one by one during full blossom, then the maternal kraft paper bag fold closure after pollination is made regular check on, performed
Insect protected, mould proof work, cut receipts when cenospecies are ripe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610886258.5A CN106386467A (en) | 2016-10-11 | 2016-10-11 | Efficient stamen killing method for japonica rice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610886258.5A CN106386467A (en) | 2016-10-11 | 2016-10-11 | Efficient stamen killing method for japonica rice |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106386467A true CN106386467A (en) | 2017-02-15 |
Family
ID=59228996
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610886258.5A Pending CN106386467A (en) | 2016-10-11 | 2016-10-11 | Efficient stamen killing method for japonica rice |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115474547A (en) * | 2022-11-01 | 2022-12-16 | 吉林省农业科学院 | Method for improving hybridization rate of millet |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430833A (en) * | 2013-08-23 | 2013-12-11 | 江西省农业科学院水稻研究所 | Novel male-killing method for sexual hybridization of rice |
| CN103733984A (en) * | 2014-01-22 | 2014-04-23 | 泗洪县原种场丰收种业有限公司 | Castration method for rice hyberdization |
| CN104145758A (en) * | 2014-07-23 | 2014-11-19 | 陈益楷 | Hybrid rice cultivation method |
| CN104885928A (en) * | 2015-06-14 | 2015-09-09 | 安徽徽大农业有限公司 | Rice hybridization and castration method |
-
2016
- 2016-10-11 CN CN201610886258.5A patent/CN106386467A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430833A (en) * | 2013-08-23 | 2013-12-11 | 江西省农业科学院水稻研究所 | Novel male-killing method for sexual hybridization of rice |
| CN103733984A (en) * | 2014-01-22 | 2014-04-23 | 泗洪县原种场丰收种业有限公司 | Castration method for rice hyberdization |
| CN104145758A (en) * | 2014-07-23 | 2014-11-19 | 陈益楷 | Hybrid rice cultivation method |
| CN104885928A (en) * | 2015-06-14 | 2015-09-09 | 安徽徽大农业有限公司 | Rice hybridization and castration method |
Non-Patent Citations (1)
| Title |
|---|
| 余飞宇等: "水稻杂交新方法-剪颖喷水去雄法的研究", 《上海农业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115474547A (en) * | 2022-11-01 | 2022-12-16 | 吉林省农业科学院 | Method for improving hybridization rate of millet |
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Application publication date: 20170215 |
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