CN106361678A - Composition with skin anti-aging effect and application of composition to cosmetics - Google Patents
Composition with skin anti-aging effect and application of composition to cosmetics Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/56—Compounds, absorbed onto or entrapped into a solid carrier, e.g. encapsulated perfumes, inclusion compounds, sustained release forms
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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Abstract
The invention relates to a composition with a skin anti-aging effect and application of the composition to cosmetics. The composition uses common milfoil extracts, tea extracts, fullerene, bamboo fungus extracts and sturgeon caviar as major ingredients; by aiming at the main mechanism of skin aging, a complete solution is provided; all ingredients have the cooperative and synergistic effects; the efficacy of regulating the cell gene expression, resisting ultraviolet rays, clearing away free radicals, resisting inflammation, inhibiting MMPs expression, preserving moisture, supplementing the extracellular matrix and the like is achieved; the anti-aging effect is achieved. The composition is applied in cosmetics in forms of cream, emulsion, solutions, gels and aerosols.
Description
Technical field
The invention belongs to the technical field of cosmetics and in particular to a kind of compositionss of delay skin aging effect and its
Utilization in cosmetics.
Background technology
Skin aging is a unrestrained long and complex evolution process, scientists propose multiple with regard to skin aging mechanism
Theory, relatively more representational have: skin aging gene regulation theory, the free radical theory of skin aging, skin aging
Metabolic disorder theory, aging theory of photostimulation of skin aging etc..
Skin aging gene regulation theory thinks that skin aging is due to synthesis suppression in Skin Cell dna and mitochondrial dna
Thing gene expression processed increases, and many genes relevant with cytoactive are suppressed, and oxidative stress affects on the damage of dna
It replicates, transcribes and expresses, therefore gene regulation is the basic reason of skin aging.The free radical theory of skin aging thinks old and feeble
During degeneration change be that the illeffectss of free radical due to producing in cell normal metabolic processes cause.Organism
Aging course be body histiocyte constantly produce free radical accumulation result, excessive free radical can cause dna damage
Wound, thus leading to be mutated, can make the many kinds of substance in cell aoxidize, and infringement biomembrane is additionally it is possible to make protein, nucleic acid etc.
Macromolecules cross-linking, affects its normal function, leads to the cross-linked polymeric of collagen protein, can make collagen protein decreased solubility, elasticity
Reduce and be hydrated into decline, lead to that skin loses tension force and wrinkle increases, skin resistance declines, and produces the skins such as mottle
Aging phenomenon.Photoaging theory thinks that illumination is one of major reason of skin aging, can be caused skin by ultraviolet radioactive for a long time
Skin photoaging, its mechanism is about: first, ultraviolet irradiates and reduces immunity by the suppression approach of inducing antigen irritant reaction
Response, the keratinocyte in such as inducible epidermis, melanocyte, langerhans celles, fibroblast in corium, interior
Chrotoplast etc. produces cytokines as various in il-1, il-6, egf, tnf etc., and they pass through to suppress the propagation of keratinocyte
Reduce the reactivity that epidermis stimulates to external world.Second, ultraviolet irradiate can active cell surface associated receptor, start uv radiation phase
OFF signal conducts, and activates specific transcription factor, and induction produces cytokine profiles, adjusts expression (the mainly mmps of target gene
And collagen protein), show as promoting the synthesis of dermal matrix matrix metalloproteinase mmps.As mmps activity increases, can draw
Play the destruction of collagen net, the accumulation of abnormal elastic fibrous matter finally causes the tissue pathologies change of skin photoage, lead to
The generation of photoaging.3rd, ultraviolet (uv) radiation can produce highly reactive active oxygen (ros) i.e. free radical and various cells
Inner structure interacts and causes cell and the damage of tissue, shows as Secretion of Inflammatory Factors increase induction dna and damages and cell
The tune a plurality of approach such as die causes skin lesion.
Anti-aging product in the market mainly for free radical resisting, mainly adopt vitamin e, vitamin c and other
Antioxidant and enzyme antioxidant, vitamin series raw material and other antioxidant existence and stabilities are poor, easy to change, clear
Except itself being also consumed while free radical, the shortcomings of persistency difference；And enzyme antioxidant has activity in cosmetics
Difference, molecular weight is big, is difficult the shortcomings of absorb.And cosmetics single function in the market, do not consider to lead to skin to decline
The old multiple factors producing and multiple biological respinses of free yl induction, therefore do not have good anti-aging effects.And delay skin
Skin aging is eternal the pursuing a goal of the mankind, and on current Cosmetic Market, anti-aging product accounts for the 50% of Cosmetic Market
More than, therefore, people have urgent demand to the anti-aging product of novel multi-effect.
Content of the invention
For being further elucidated with the present invention, technical term used in subject application is defined as follows, unless otherwise indicated.
Definition:
Extract: refer to a kind of by by the material to be extracted soak or be mixed in the solution obtained in solvent.Extraction
Take process can select be suitable for solvent with the help of carry out, such as: water, ethanol, ethanol/water mixture, methanol, propanol, butanol,
Chloroform, acetone or other organic solvents, and pass through isolation, diafiltration, decoction, counter-current extraction, ultrasound wave, microwave, supercritical fluid
The method of extraction obtains extract, extract can evaporation and concentration further, obtain extractum and the extract of drying.
Common milfoil extract: extract for raw material and obtain with (achillea millefolium) stem of common milfoil, leaf, flower,
The ratio of the wherein stem and leaf of common milfoil and common milfoil flower is 3:1.Its contain Millefolium element, α-Camphora, deacetylated matricin, equisetic acid,
α-furyl acid, succinic acid, fumaric acid, succinic acid, Fumaric acid, chlorogenic acid, inulin, cineole, myristic acid, sub- oil
Acid, sitosterol, stigmasterol, several amino acids (lysine, histidine, arginine, glutamic acid, Phenylalanine, arginine, cheese ammonia
Acid, leucine, aspartic acid, proline etc.), alkaloid, Coumarinses, flavonoid, sesquiterpenoidss, volatile oil etc..Have anti-
Inflammation, sterilization, the anti-ageing effect of waiting for a long time.
Tea extract: extracted for raw material with semi-fermented tea (TIEGUANYIN) and obtain.It contains polysaccharide, flavone, flavonoid, flavane
Alcohol, catechin, anthocyan composition and aroma volatile, have the work(such as antioxidation, defying age, sterilization antiinflammatory, uvioresistant
Effect.
Fullerene: fullerene (fullerene) is that a class is made up of carbon, and with spherical, ellipticity, or tubular structure is deposited
Material, it is referred to as " absorb free radical sponge ", and itself is difficult to be consumed when removing free radical.Due to fullerene
, so as to have special physics and chemical property, such as catalysis activity, carrier function, biological activity etc., in change for hollow cage-shape structure
The effects such as cosmetic aspect has free radical resisting, antioxidation, light speckles, beautification and white with suppression inflammation.Additionally, it also has substantially to mmps
Inhibitory action, the extracellular matrixs such as collagen protein, elastin laminin and laminin，LN can be protected not to be degraded.
Caulis Bambusae In Taeniam extract: extracted for raw material with the sporophore of Dictyophora rubrovalvata (dictyophora rubrovolvata)
Arrive, it is rich in dictyophora fungus polysaccharide and several amino acids, have regulation immunity of organisms, antioxidation, antiinflammatory antibacterial the effects such as.
Acipenser Sinensis caviar extract: it is the extract obtaining from the ovum from Acipenser Sinensis (acipenser sturio), main
Want composition to have the carbohydrate contents such as glucose, Fructose, sucrose and glycogen, form close lipid (unsaturated fatty acidss with human body skin
Acid, sterol, phospholipid etc.), substantial amounts of small molecule oligopeptide and polypeptide and several amino acids (isoleucine, lysine, Methionine,
Cystine, Phenylalanine, tyrosine, threonine, tryptophan, L-Valine, arginine, histidine, alanine, aspartic acid,
Glutamic acid, glycine, proline and serine) and vitamin a, d, b1, b2 and h.The amino acid and protein that it is rich in is permissible
There is provided nourishing for skin, the unsaturated fatty acidss of high-load can moisten the aging skin of drying, vitamin can recover skin and live
Power.
The present invention provides a kind of cosmetic industry of new delay skin aging to become subassembly, to overcome prior art to exist
Defect.The purpose of the present invention is the mechanism being formed from skin aging, from regulation and control aging gene expression, prevents ultraviolet pair
The damage of skin, removes harmful free radical, strengthens cell-proliferation activity, reconstructs extracellular matrix, reduces mmps albuminoid enzyme
Activity prevents, the extracellular matrix such as multiple collagen protein, proteoglycan, elastin laminin and laminin，LN such as type dropped
Solution, reduces the inflammatory factor level inducing because of skin aging, the required several amino acids of replenishing collagen synthesis, promotes glue
Former albumen synthesis, adjusts the metabolism of Skin Cell, activated cell vigor.Additionally, strengthening skin in skin outer layer
Barrier function, improves the ability that Skin Cell resists environmental damage, improves the water content of skin, recovers elasticity of skin, realizes complete
Orientation delay skin aging.Not only make each composition give full play to self-acting, and also achieve the effect of Synergistic.
The technical scheme is that
A kind of compositionss with skin anti-aging effect: be made up of following parts by weight of component:
Functional component: 0.01～100 part of common milfoil extract, 0.01～20 part of tea extract, Caulis Bambusae In Taeniam extract 0.001～
100 parts, fuller olefinic carbon 60 .0001～10 part, 0.01～50 part of Acipenser Sinensis caviar extract；And c in fullerene60: c70=0.01～
0.5:1
Its processing technology is: weighs Caulis Bambusae In Taeniam extract, fullerene, Acipenser Sinensis caviar extract loading ball milling according to the above ratio
In tank, add zirconium pearl, ball milling 0.5～2h.Add common milfoil extract, tea extract, ball milling 5～10min.
Preferably it is combined as:
Functional component: 0.1～80 part of common milfoil extract, 0.05～20 part of tea extract, Caulis Bambusae In Taeniam extract 0.01～100
Part, fuller olefinic carbon 60 .001～10 part, 0.1～50 part of Acipenser Sinensis caviar extract；And c in fullerene60: c70=0.01～0.5:1
Be more highly preferred to is combined as:
Functional component: 1～50 part of common milfoil extract, 1～10 part of tea extract, 10～50 parts of Caulis Bambusae In Taeniam extract, fowler
0.001～5 part of alkene, 0.5～30 part of Acipenser Sinensis caviar extract；And c in fullerene60: c70=0.01～0.5:1
Another object of the present invention is a kind of application in cosmetics for compositionss of skin anti-aging effect:
Described cosmetics are cream agent, emulsion, solution, gel, aerosol form.
A kind of ratio 0.001%～60% of the cosmetics gross weight shared by compositionss of described skin anti-aging effect.
Preferably ratio accounts for the 0.01～20% of cosmetics gross weight for said composition.
Preferred ratio accounts for the 0.1～10% of cosmetics gross weight for said composition.
The present invention develops a kind of new compositionss with skin anti-aging effect, and it is directed to the many of skin aging generation
Kind of mechanism is compounded, and compounding composition is innovated, obtain having synergistic function formula combination, this combination
Anti-aging effects are much better than each single component sum in formula.Simple production process of the present invention, suitable large-scale production, and
The dissolubility of fullerene can be lifted, strengthen senile-resistant efficacy, improve product stability；Functional component is promoted to form intermolecular friendship
Connection, improves molecular weight, strengthens moisturizing activity；Promote to interact between functional component, produce the effect of Synergistic；Promote
Functional component is embedded in macromolecular complex, plays the effect of slow release.And adopt all kinds of cosmetics that said composition is prepared equal
There is good senile-resistant efficacy.
Specific embodiment
Example below is described in detail to the new method of the present invention, but is not intended to limit the scope of the present invention.
Embodiment 1:
A kind of compositionss with skin anti-aging effect, are made up of following parts by weight of component:
Functional component: 10 parts of common milfoil extract, 1 part of tea extract, 10 parts of Caulis Bambusae In Taeniam extract, fuller olefinic carbon 60 .001 part, sturgeon
0.5 part of fish caviar extract；And c in fullerene60: c70=0.05:1.
Its processing technology is: weighs Caulis Bambusae In Taeniam extract, fullerene, Acipenser Sinensis caviar extract loading ball milling according to the above ratio
In tank, add zirconium pearl, ball milling 0.5h.Add common milfoil extract, tea extract, ball milling 5min.
Above-described embodiment preparation method is simple, with low cost, suitable large-scale promotion, prepared anti-apolexis composition, tool
There is the features such as anti-aging effects are good, safe, gentle, effect is comprehensive.
Embodiment 2:
A kind of compositionss with skin anti-aging effect, are made up of following parts by weight of component:
Functional component: 20 parts of common milfoil extract, 10 parts of tea extract, 20 parts of Caulis Bambusae In Taeniam extract, fuller olefinic carbon 60 .1 part, sturgeon
5 parts of fish caviar extract；And c in fullerene60: c70=0.5:1.
Its processing technology is: weighs Caulis Bambusae In Taeniam extract, fullerene, Acipenser Sinensis caviar extract loading ball milling according to the above ratio
In tank, add zirconium pearl, ball milling 1.5h.Add common milfoil extract, tea extract, ball milling 10min.
Above-described embodiment preparation method is simple, with low cost, suitable large-scale promotion, prepared anti-apolexis composition, tool
There is the features such as anti-aging effects are good, safe, gentle, effect is comprehensive.
Embodiment 3:
A kind of compositionss with skin anti-aging effect, are made up of following parts by weight of component:
Functional component: 40 parts of common milfoil extract, 5 parts of tea extract, 10 parts of Caulis Bambusae In Taeniam extract, 1 part of fullerene, Acipenser Sinensis fish
10 parts of seed beans extract；And c in fullerene60: c70=0.01:1.
Its processing technology is: weighs Caulis Bambusae In Taeniam extract, fullerene, Acipenser Sinensis caviar extract loading ball milling according to the above ratio
In tank, add zirconium pearl, ball milling 2h.Add common milfoil extract, tea extract, ball milling 10min.
Above-described embodiment preparation method is simple, with low cost, suitable large-scale promotion, prepared anti-apolexis composition, tool
There is the features such as anti-aging effects are good, safe, gentle, effect is comprehensive.
Embodiment 4:
A kind of compositionss with skin anti-aging effect, are made up of following parts by weight of component:
Functional component: 10 parts of common milfoil extract, 5 parts of tea extract, 10 parts of Caulis Bambusae In Taeniam extract, fuller olefinic carbon 60 .1 part, Acipenser Sinensis
5 parts of caviar extract；And c in fullerene60: c70=0.05:1.
Its processing technology is: weighs Caulis Bambusae In Taeniam extract, fullerene, Acipenser Sinensis caviar extract loading ball milling according to the above ratio
In tank, add zirconium pearl, ball milling 2h.Add common milfoil extract, tea extract, ball milling 10min.
Above-described embodiment preparation method is simple, with low cost, suitable large-scale promotion, prepared anti-apolexis composition, tool
There is the features such as anti-aging effects are good, safe, gentle, effect is comprehensive.
Embodiment 5:
A kind of compositionss with skin anti-aging effect, are made up of following parts by weight of component:
Functional component: 8 parts of common milfoil extract, 5 parts of tea extract, 10 parts of Caulis Bambusae In Taeniam extract, fuller olefinic carbon 60 .5 part, Acipenser Sinensis
3 parts of caviar extract；And c in fullerene60: c70=0.1:1.
Its processing technology is: weighs Caulis Bambusae In Taeniam extract, fullerene, Acipenser Sinensis caviar extract loading ball milling according to the above ratio
In tank, add zirconium pearl, ball milling 1h.Add common milfoil extract, tea extract, ball milling 8min.
Above-described embodiment preparation method is simple, with low cost, suitable large-scale promotion, prepared anti-apolexis composition, tool
There is the features such as anti-aging effects are good, safe, gentle, effect is comprehensive.
With regard to the application in cosmetics for the compositionss of the present invention, carried out using embodiments of the invention 4 as example below
Describe in detail, but be not intended to limit the interest field of the present composition.Embodiment 6:
A kind of facial cream containing said composition:
Embodiment 7:
A kind of emulsion containing said composition
Embodiment 8:
A kind of gel containing said composition:
Embodiment 9:
A kind of astringent containing said composition:
Embodiment 10:
A kind of essence containing said composition:
Embodiment 11:
A kind of spraying containing said composition:
Wherein, the cosmetics described in embodiment 6-11 taking embodiment 4 as a example illustrate, such as by the compositionss of embodiment 4
It is replaced by the combination in any in the claims in the present invention and content, then the other compositions in cosmetic formulations can be protected in claim
Make the appropriate adjustments in the range of shield.
Enter for measuring difference between the effect in terms of defying age and single functional component for the anti-apolexis composition of the present invention
Go following experimentation:
Following experiments are all using following laboratory sample: be substituted for 2% embodiment 4 in embodiment 11 formula single respectively
Functional component (i.e. this single functional component content is identical with the component content of the same race in 2% embodiment 4), that is, contained
0.66% spraying of common milfoil extract, the spraying containing 0.33% tea extract, the spray containing 0.66% Caulis Bambusae In Taeniam extract
The spraying of mist and the spraying containing 0.33% Acipenser Sinensis caviar extract and 0.0066% fullerene is (because fullerene is molten in water
Solution property is bad, therefore its less stable).
1st, the suppression ratio to mmp-1 expression
The one of the main reasons of skin aging is that the structure of skin corium there occurs change.Due to the effect of some extraneous factors,
Swash intravital matrix metalloproteinase (mmps), may result in the collagen protein supporting skin texture in dermis of skin and elastic egg
It is excessively degraded in vain, so that shrinkage, the senile symptom such as nonelastic in skin.Mmp-1 also known as collagenase -1, degradable,
, and x Collagen Type VI gelatin and proteoglycan.By testing these samples, these samples are assessed to the suppression ratio of mmp-1
Anti-aging effects.
Fibroblast is inoculated in 12 porocyte culture plates, each hole contains 0.75 × 105Individual cell, and no
Hungry culture 24 hours in blood serum medium.Rinse the cell through hungry culture with pbs, and with treatment with ultraviolet light (40mj).
Then, in 48 hours, addition test sample 2 times in cell.With in test kit (biotrak, rpn2610) measurement culture medium
Detached mmp-1.Assess the power of senile-resistant efficacy by the expression inhibiting rate calculating mmp-1.Computing formula is as follows:
Suppression ratio=(a-b)/a*100%
A: be not added with test sample, but after ultra-vioket radiation mmp-1 expression
B: add test sample and after ultra-vioket radiation mmp-1 expression
Shown in result table 1.
The suppression ratio that the different test sample of table 1 is expressed to mmp-1
| Test sample | Volume (μ l) | Suppression ratio (%) |
| 0.66% common milfoil extract spraying | 50 | 8.4 |
| 0.33% tea extract spraying | 50 | 6.7 |
| 0.66% Caulis Bambusae In Taeniam extract spraying | 50 | 10.9 |
| 0.33% Acipenser Sinensis caviar extract spraying | 50 | 8.7 |
| 0.0066% fullerene spraying | 50 | 2.4 |
| Embodiment 11 | 5 | 89.4 |
As can be seen from the above table, the suppression ratio that 6 kinds of testing samples are expressed to mmp-1 is different, but embodiment 11 sample
Apparently higher than other 5 testing samples,, under the concentration of 10 μ l, suppression ratio reaches 89.4%, far for it for the suppression that mmp-1 is expressed
It is sprayed at the suppression ratio under 50 μ l concentration and this 5 kinds suppression ratio sums sprayed far above other 5 kinds, illustrate that embodiment 11 is right
Mmp-1 expression has significant inhibitory action, and wherein each component has good syneryistic effect.
2nd, the synthesis promotion rate of collagen type:
The human fibroblasts of culture in the dmem culture medium containing 10%fbs are seeded in 24 orifice plates, after cell attachment,
Carry out culture medium exchange with the dmem culture medium containing 0.25%fbs and 250 μm of magnesium ascorbyl phosphate, be separately added into above-mentioned 6
Plant testing sample.After culture three days, reclaim culture medium supernatant, centrifugation, measure the collagen type in gained supernatant.
Evaluation methodology with regard to the biosynthesis ability of collagen type in cell is using " ria-gnost p p (p
P) measure test kit " measure supernatant in secretion collagen type terminal peptide (procollagen type-
Content peptide: referred to as p p).By the synthesis promotion rate of collagen type and then assess the anti-ageing of these samples
The power of old effect.Computing formula is as follows:
Promotion rate=(a-b)/b*100%
A: add the collagen type growing amount after test sample
B: be not added with the collagen type growing amount of test sample
The results are shown in Table 2:
The different synthesis promotion rate to collagen type for the test sample of table 2
| Test sample | Concentration (μ l) | Promotion rate (%) |
| 0.66% common milfoil extract spraying | 50 | 10.5 |
| 0.33% tea extract spraying | 50 | 12.1 |
| 0.66% Caulis Bambusae In Taeniam extract spraying | 50 | 15.3 |
| 0.33% Acipenser Sinensis caviar extract spraying | 50 | 16.3 |
| 0.0066% fullerene spraying | 50 | 1.7 |
| Embodiment 11 | 10 | 178.2 |
As can be seen from Table 2, the spraying of various single functional components is respectively provided with necessarily to collagen type growing amount
Promotion activity but all relatively low, be below 20% at the concentration tested.If the promotion of total five kinds of each single functional component sprayings
If rate, its promotion rate is 59.9%, and the promotion rate of embodiment 2 is 178.2%, is about the former 3 times, and this explanation is passed through
After compounding, there is synergism in the functional component in five kinds of extracts, mutually strengthen, and promotes cell that collagen type is divided
Secrete, improve collagen type growing amount, thicken extracellular matrix, and then realize anti-aging effects.
3rd, antioxidant activity
Measuring principle: dpph (1,1- diphenyl -2- trinitrophenyl-hydrazine) be a kind of stable proton centered on nitrogen from
By base, its ethanol solution is in purple, and has strong absorption at 517nm, and radical scavenging activity with the light absorption value at 517nm is in
Linear correlation, is the typical method of the in vitro anti-oxidation of assessment antioxidant content.
Do blank with 300 μ l 50% ethanol, draw the sample to be tested of different volumes to 120 μm of ol/l of 250 μ l
In dpph solution (dpph reagent is now with the current), supplied with 50% ethanol less than 300 μ l, react 30min at room temperature, in
517nm measures light absorption value (a), replaces sample to add in the dpph solution of same volume with 50% ethanol of same dose, measures
Dpph maximum light absorption value (a0).Equivalent prepare liquid is separately taken to be added in 50% ethanol to eliminate the impact to light absorption value for the sample itself
(aSample).It is repeated 3 times, the power of Scavenging activity is represented with clearance rate, is calculated as follows:
Experimental result is shown in Table 3:
The different scavenging capacity to dpph for the test sample of table 3
| Test sample | Concentration (μ l) | Clearance rate (%) |
| 0.66% common milfoil extract spraying | 50 | 20.5 |
| 0.33% tea extract spraying | 50 | 21.1 |
| 0.66% Caulis Bambusae In Taeniam extract spraying | 50 | 10.3 |
| 0.33% Acipenser Sinensis caviar extract spraying | 50 | 9.5 |
| 0.0066% fullerene spraying | 50 | 5.7 |
| Embodiment 11 | 5 | 95.1 |
As can be seen from Table 3, the spraying of various single functional components all has certain scavenging capacity to dpph, if total
Five kinds of each single functional components are sprayed at and add the clearance rate under 50 μ l to be 67.1%, and embodiment 11 is in the situation adding 5 μ l
Under, its clearance rate has just reached 95.1%, and after compounding, the functional component in five kinds of extracts occurs collaborative work for this explanation
With mutually strengthening, improve the scavenging capacity of dpph that is to say, that its antioxidation, senile-resistant efficacy are strengthened.
4th, moisturizing activity
The aging of skin is in close relations with the moisture of skin, and skin hydropenia can accelerate skin aging, and moisture of skin
Content abundance can make skin tender flexible, slow down aging.Test 30 using universally acknowledged corneometer- capacitance method
The change of volunteer's moisture content of skin, to assess the performance of keeping humidity of testing sample.
30 experimenters are the age in the female volunteers of 20～40 years old health, no skin medical history.Volunteer before testing
The test environment (temperature is 20 DEG C ± 1 DEG C, and relative humidity is 40%～60%) of constant temperature and humidity, rest 30min need to be entered in advance.
Define several area identical points in left forearm, as using region.The point of right forearm same position is tested as blank
When, the corresponding region of left and right forearm is tested simultaneously.The consumption of each point is 5mg/cm2.After 2h is smeared in observation, moisture content of skin
Change, to represent the performance of keeping humidity of testing sample.
Moisturizing rate=(a-b)/b*100%
A: add skin moisture content after test sample.
B: be not added with test sample skin moisture content.
The results are shown in Table 4:
The moisturizing activity of the different test sample of table 4
| Test sample | Moisturizing rate (%, 2h) | Moisturizing rate (%, 3h) |
| 0.66% common milfoil extract spraying | 1.5 | 0.5 |
| 0.33% tea extract spraying | 1.8 | 1.1 |
| 0.66% Caulis Bambusae In Taeniam extract spraying | 10.2 | 4.3 |
| 0.33% Acipenser Sinensis caviar extract spraying | 6.7 | 3.5 |
| 0.0066% fullerene spraying | 1.2 | 0.7 |
| Embodiment 11 | 32.7 | 30.1 |
As can be seen from Table 4, the moistening effect of the spraying of five kinds of single components is different, wherein Caulis Bambusae In Taeniam extract
Effect preferably, can reach 10%, others are weaker.And embodiment 11 moisturizing rate after 2h reaches 32.7%, it is approximately various single
1.5 times of the moisturizing rate sum of functional component spraying, and the as shown by data after 3h, the moisturizing of various single functional component sprayings
Rate all significantly decreases, and the moisturizing rate of embodiment 11 declines and inconspicuous (dropping to 30.1% from 32.7%), is approximately each
3 times of single component moisturizing rate sum.After compounding, there is synergism in the functional component in five kinds of extracts for this explanation,
Mutually strengthen, improve the moisturizing rate of product that is to say, that its senile-resistant efficacy is strengthened.
Described above illustrate and describes the preferred embodiments of the present invention, as previously mentioned it should be understood that the present invention not office
Be limited to form disclosed herein, be not to be taken as the exclusion to other embodiment, and can be used for various other combinations, modification and
Environment, and can be changed by the technology or knowledge of above-mentioned teaching or association area in invention contemplated scope described herein
Dynamic.And the change that those skilled in the art are carried out and change without departing from the spirit and scope of the present invention, then all should be appended by the present invention
In scope of the claims.
Claims (9)
1. a kind of compositionss with skin anti-aging effect are it is characterised in that described compositionss: by following parts by weight of component
Composition: 0.01～100 part of common milfoil extract, 0.01～20 part of tea extract, 0.001～100 part of Caulis Bambusae In Taeniam extract, fullerene
0.0001～10 part, 0.01～50 part of Acipenser Sinensis caviar extract.
2. as claimed in claim 1 a kind of compositionss with skin anti-aging effect it is characterised in that described combination
Thing: be made up of following parts by weight of component: 0.1～80 part of common milfoil extract, 0.05～20 part of tea extract, Caulis Bambusae In Taeniam extract
0.01～100 part, fuller olefinic carbon 60 .001～10 part, 0.1～50 part of Acipenser Sinensis caviar extract.
3. there are the compositionss of skin anti-aging effect it is characterised in that described compositionss as claim 2 is a kind of: by with
Lower parts by weight of component composition: 1～50 part of common milfoil extract, 1～10 part of tea extract, 10～50 parts of Caulis Bambusae In Taeniam extract, fowler
0.001～5 part of alkene, 0.5～30 part of Acipenser Sinensis caviar extract.
4. a kind of compositionss with skin anti-aging effect as described in claim 1,2 or 3 are it is characterised in that fullerene
In, c60: c70=0.01～0.5:1.
5. application in cosmetics for a kind of compositionss with skin anti-aging effect as claimed in claim 4, its feature
It is: described cosmetics are cream agent, emulsion, solution, gel, aerosol form.
6. application in cosmetics for a kind of compositionss with skin anti-aging effect as claimed in claim 5, its feature
It is: the 0.001%～60% of the cosmetics gross weight shared by compositionss of described a kind of skin anti-aging effect.
7. application in cosmetics for a kind of compositionss with skin anti-aging effect as claimed in claim 6, its feature
It is: the 0.01～20% of the cosmetics gross weight shared by compositionss of described a kind of skin anti-aging effect.
8. application in cosmetics for a kind of compositionss with skin anti-aging effect as claimed in claim 7, its feature
It is: the 0.1～10% of the cosmetics gross weight shared by compositionss of described a kind of skin anti-aging effect.
9. a kind of processing technology of the compositionss with skin anti-aging effect it is characterised in that: processing technology is: claims in proportion
Take Caulis Bambusae In Taeniam extract, fullerene, Acipenser Sinensis caviar extract to load in ball grinder, add zirconium pearl, ball milling 0.5～2h, add Europe
Achillea millefolium extract, tea extract, ball milling 5～10min.
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| CN107375043A (en) * | 2017-07-14 | 2017-11-24 | 高飞 | A kind of application of composition containing small-micelle water and said composition in cosmetics |
| CN107929131A (en) * | 2017-12-08 | 2018-04-20 | 广州智媛生物科技有限公司 | Anti-apolexis composition and application thereof |
| CN110403890A (en) * | 2018-04-30 | 2019-11-05 | 科丝美诗株式会社 | The composition for beautifying skin including sturgeon Egg extracts and sturgeon amniotic fluid extract |
| CN110934787A (en) * | 2019-11-27 | 2020-03-31 | 陈少威 | Anti-aging composition and preparation method thereof |
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| CN107375043A (en) * | 2017-07-14 | 2017-11-24 | 高飞 | A kind of application of composition containing small-micelle water and said composition in cosmetics |
| CN107929131A (en) * | 2017-12-08 | 2018-04-20 | 广州智媛生物科技有限公司 | Anti-apolexis composition and application thereof |
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| CN110934787B (en) * | 2019-11-27 | 2022-04-19 | 陈少威 | Anti-aging composition and preparation method thereof |
| CN111728901A (en) * | 2020-07-13 | 2020-10-02 | 植物医生(广东)生物科技有限公司 | Anti-aging composition and preparation method and application thereof |
| CN113197785A (en) * | 2021-06-22 | 2021-08-03 | 杭州友丽医疗科技(集团)有限公司 | Anti-wrinkle and anti-redness regeneration composition, essence and preparation method thereof |
| CN113262188A (en) * | 2021-06-23 | 2021-08-17 | 上海林清轩生物科技有限公司 | Plant extract for relieving eczema and preparation method and application thereof |
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