CN106359195A - Breeding method for fingerlings of Onychosotoma gerlachi - Google Patents
Breeding method for fingerlings of Onychosotoma gerlachi Download PDFInfo
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- 241001504946 Onychostoma gerlachi Species 0.000 title abstract 3
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- MJVAVZPDRWSRRC-UHFFFAOYSA-N Menadione Chemical compound C1=CC=C2C(=O)C(C)=CC(=O)C2=C1 MJVAVZPDRWSRRC-UHFFFAOYSA-N 0.000 claims description 3
- 229930182555 Penicillin Natural products 0.000 claims description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 3
- -1 SANHUANGFEN Chemical compound 0.000 claims description 3
- 239000011230 binding agent Substances 0.000 claims description 3
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 3
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- 241001247197 Cephalocarida Species 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a breeding method for fingerlings of Onychosotoma gerlachi. The method comprises the following steps: A, brood fish is fed; B, the brood fish is selected; C, the brood fish selected in the step B is put in a spawning pond to be fed; D, inducing agents are injected to the brood fish in the spawning pond; E, artificial incubation is carried out under a sun-shielding and overshadowing environment; F, fish eggs are kept in a hatching pond for 80h, then the fish can be hatched out of membranes, after absorption is carried out for 2 to 4 days, fish fries float upwards and flatly swim; G, the fish fries obtained in the step F are released, wherein the release intensity is 100000 fries per mu (a unit of area equal to 0.0667 hectares); the release time is morning or evening, the direct light is not required, the temperature difference of release water temperature is less than or equal to 2 DEG C, the number of rotifers in water of a release pond reaches the peak; the requirements of the release pond are that the water depth is 60 to 80cm, the dissolved oxygen is greater than or equal to 5mg/L, filtering facilities are configured in a water inlet and a drain outlet, and an aerator is configured in the pond; throwing feeding is performed; H, diseases are prevented and controlled. By adopting the method disclosed by the invention for breeding the fingerlings of the Onychosotoma gerlachi, the yield and the survival rate of the fries can be greatly improved to lower the breeding cost.
Description
Technical field
The invention belongs to onychostoma simus cultural technique field, especially a kind of offspring breeding method of vacricorhinus gerlachi.
Background technology
Vacricorhinus gerlachi, Cyprinidae, subfamily, onychostoma simus belongs to, and is distributed mainly on the Zhujiang River, Yuanjiang River, the Lancang River and Hainan Island each
Water system.Vacricorhinus gerlachi inhabits that current are more rapid mostly, in the section of substrate pebbly river, happiness is cruised in the bottom of water.Every year
Trace back in groups before and after rainwater section river upstream, before and after the beginning of autumn then downstream and under, winter survives the winter in riprap at the deep water in rivers mainstream.
Often with the algae on sharp cutin lower jaw shovel food rock, and the fragment of the chironomus larvas, oligochaetes and higher plant of lack of appetite amount.
Intensity of feeding maximum is in 3-4 month, and winter and breeding season are general all seldom or stop ingesting.3 ages in winter reached sexual maturity, produced
The ovum phase is longer, and the Yangtze river basin is the 4-6 month, and Pearl River Delta is the 2-3 month.Spawning ground mostly is at gravel and the torrent at sandy beach, and ovum adheres to
Water-bed gravel is hatched.
The fry of the vacricorhinus gerlachi of existing artificial cultivation is fished from local fisherman and fishes for, and fingerling source mode is single,
Fish body mechanical damage is more serious, easily infection fish moldss and generation stress, and survival rate is low.
Content of the invention
It is an object of the invention to provide a kind of offspring breeding method of vacricorhinus gerlachi, using artificial breeding seed, carry
The yield of high seed and survival rate.
The object of the present invention is achieved like this: a kind of offspring breeding method of vacricorhinus gerlachi, comprises the following steps:
A, fish will be planted put into kind of fishpond and feed, kinds fishpond will be rectangle pond, depth of water 60cm to 100cm, 17 DEG C to 22 DEG C of water temperature,
Dissolved oxygen is more than or equal to 5mg/l, and discharge meets water per hour and exchanges 2 times；
B, to mating period when select kind of a fish: on milter mouth pearl star substantially, appearance not damaged, no deformity, light pressure abdominal part can flow out
Milky seminal fluid, the long 12cm to 18cm of body, body weight 120g to 180g；Raun abdominal part expands, soft, body surface not damaged, and scale is complete
Whole, gonopore pinkiness, the long 17cm to 24cm of body, body weight is more than or equal to 200g；
C, the kind fish picking out step b are put into spawning inducing pool and feed, spawning inducing pool floor space 3 to 4, and discharge meets often little
Shi Chishui at least exchanges 2 times, 17 DEG C to 22 DEG C of water temperature, and dissolved oxygen is more than or equal to 5mg/l；
D, in spawning inducing pool kind fish injection odinagogue；
E, artificial incubation:
E1, when kind of a fish starts mutually to chase, milter is pulled out, dries the moisture on gonopore, then press abdominal part, allow it
Seminal fluid flows out, with syringe by seminal fluid collecting；After treating seminal fluid collecting well, then raun is pulled out, with towel by the moisture of body
Wipe, then press abdominal part, fish roe is clamp-oned in china bowl；
E2, seminal fluid is clamp-oned china bowl, the seminal fluid that the ratio of seminal fluid and fish roe meets 1 milter is mixed with the fish roe of 2 to 3 rauns
Close；Pour the normal saline 2ml that mass concentration is 0.9% again into, stirring, after mix homogeneously, add clear water, from being stirred to addition
Clear water is less than 30 seconds interval time, quick and graceful in the movements, the tenderness of stirring, adds the ovum of clear water to start to be fertilized, and imbibition continues
Filling clear water；
After e3, placement 1min to 2min in china bowl, then move in big basin, after fish roe absorbs water fertilization 1h in big basin, will be subject to
Smart ovum is evenly coated on the artificial fish nest in hatchery, and is passed through flowing water into hatchery and is hatched, the water temperature 17 of hatchery
DEG C -22 DEG C, dissolved oxygen is more than or equal to 5mg/l, and after eyed eggs, dissolved oxygen is more than or equal to 7mg/l；
This step is all carried out in the environment of lucifuge is sheltered from heat or light；
F, fish roe 80h in hatchery got final product incubation of membrane, and the fry after membrane is in the interior vegetative phase, through the suction of 2-4 days
Receive, fry floats and puts down trip；
G, the fry obtaining in step f is thrown in, thrown in density: 90000 tails/mu to 110000 tails/mu；Release time:
Morning or dusk, do not have direct light to shine, throw in the water temperature temperature difference and be less than or equal to 2 DEG C, the wheel animalcule quantity thrown in pond water reaches
To peak period；Throw in pond to require: depth of water 60cm-80cm, dissolved oxygen is more than or equal to 5mg/l, enters the configurating filtered facility of discharge outlet,
Aerator is configured in pond；Fed after input；
H, control and prevention of disease.
Further, in step d, the kind fish good to Maturity injects an odinagogue, and odinagogue adopts lrh-a2, hcg
And dom, the injection volume of every kilogram of raun is the dom of the hcg+2mg of lrh-a2+1000iu of 5ug, the injection volume of every kilogram of milter
Halve compared with raun；
To imperfect kind of fish injection odinagogue twice of Maturity, inject for the first time: every kilogram of raun injects the lrh-a2 of 10ug,
The injection volume of every kilogram of milter halves compared with raun；Inject for second: the hcg of the lrh-a2+1000iu of every kilogram of raun injection 5ug
The dom of+3mg, the injection volume of every kilogram of milter halves compared with raun；The time interval of double injection 12 hours.
Further, in step g, fed as bait using eel powder, bean milk, milk or egg yolk, every ten thousand tail fishes
Seedling feeds bait 5g every time, feeds 6-8 time daily, it then follows throw multiple principle less.
Further, after step e1, milter and raun are invaded bubble with the penicillin of 400 units, more separately put into shield in puerperal
The recuperation of reason pond.
Further, in step h, including the prevention and control of onychostoma simus hemorrhagic disease, ichthyophthiriasiss and anchor head pulicosis,
Onychostoma simus hemorrhagic disease: florfenicol, SANHUANGFEN, vitamin k 3 and binding agent are mixed bait and feeds；
Ichthyophthiriasiss: with copper sulfate and ferrous sulfate mixed solution full pool spilling head；
Anchor head pulicosis: when the quantity of lernaean is few, removed with tweezers；When as more in quantity, embathed using potassium permanganate solution
Sick fish.
The invention has the beneficial effects as follows: according to the life habit of vacricorhinus gerlachi, provide good growth, growth for kind of fish
Environment is it is ensured that the quality and quantity of seminal fluid and fish roe；Fertilization is operated using artificial, provides suitable environment for fertilization, significantly
Improve the success rate of fertilization；The hatching of fry and throwing in all carry out in specific environment it is ensured that the success rate hatch with
The survival rate of fry.Carry out the seed breeding of vacricorhinus gerlachi using this method, the yield of seed can be increased substantially and deposit
Motility rate, thus reduce aquaculture cost.
Specific embodiment
With reference to embodiment, the present invention is further described.
A kind of vacricorhinus gerlachi offspring breeding method of the present invention, comprises the following steps:
A, fish will be planted put into kind of fishpond and feed, kinds fishpond will be rectangle pond, length 30m in pond, width 5m, and pool inner water is deeply
60cm to 100cm, 17 DEG C to 22 DEG C of water temperature, dissolved oxygen is more than or equal to 5mg/l, and discharge meets water exchange per hour 2 times,
Configure well shade net.Additionally, at the bottom of pond can suitably place some cobblestones etc. to allow it to shake down according to simulation wild environment.
Plant fish to fish for from local fisherman, kind of fishpond is recovered body constitution, carry out raising and train so as to adapting to and accepting artificial of artitificial food
Feed, feedstuff, using the high-quality kind fish bait being of high nutritive value, can allow wild fish be transformed into from the existence accumulation of wild environment
Existence accumulation+reproduction accumulation, to greatly speed up the growth of piscinity gland.
B, to mating period when select kind of a fish: on milter mouth pearl star substantially, appearance not damaged, no deformity, light pressure abdominal part energy
Flow out milky seminal fluid, the long 12cm to 18cm of body, the long 15cm of body weight 120g to 180g, preferably body, the kind fish of body weight 150g；Raun
Abdominal part expands, soft, body surface not damaged, and scale is complete, gonopore pinkiness, the long 17cm to 24cm of body, and body weight is more than or waits
In 200g, the preferably long 20cm of body, body weight is more than the kind fish of 200g；Raun individuality is bigger, and fish brood amount is more, therefore it should select
Female kind of big fish of build.
C, the kind fish picking out step b are put into spawning inducing pool and feed, spawning inducing pool floor space 3 to 4, and discharge meets
Water at least exchanges 2 times per hour, 17 DEG C to 22 DEG C of water temperature, and dissolved oxygen is more than or equal to 5mg/l；Pool area of hastening parturition is less, so that
In pulling kind of a fish out.
D, in spawning inducing pool kind fish injection odinagogue；
E, artificial incubation: before collecting semen and fish roe, various apparatus are accounted for the hundred of complete soln quality using 10ppm(Solute mass
Very much than) kmno4 solution disinfection 10min.
E1, when kind of a fish starts mutually to chase, milter is pulled out, dries the moisture on gonopore, then press abdominal part,
Its seminal fluid is allowed to flow out, with syringe by seminal fluid collecting；After treating seminal fluid collecting well, then raun is pulled out, with towel by body
Moisture wipes, then presses abdominal part, and fish roe is clamp-oned in china bowl；By the way of two people coordinate, a people squeezes seminal fluid collecting
Extrude seminal fluid, another uses syringe collecting.It should be noted that not contacting with water with before fish roe mixing in seminal fluid during collection.Male after collection
Fish and raun are put in different buckets.
E2, seminal fluid is clamp-oned china bowl, the ratio of seminal fluid and fish roe meets the seminal fluid of 1 milter and the fish roe of 2 to 3 rauns
Mixing；Pour the normal saline 2ml that mass concentration is 0.9% again into, using feather stirring, after mix homogeneously, add clear water, from
It is stirred to addition clear water to be less than 30 seconds interval time, quick and graceful in the movements, the tenderness of stirring, add the ovum of clear water to start to be fertilized, inhale
Water expands, and continues filling clear water；
After e3, placement 1min to 2min in china bowl, then move in big basin, after fish roe absorbs water fertilization 1h in big basin, will be subject to
Smart ovum is evenly coated on the artificial fish nest in hatchery, and is passed through flowing water into hatchery and is hatched, the water temperature 17 of hatchery
DEG C -22 DEG C, dissolved oxygen is more than or equal to 5mg/l, and after eyed eggs, dissolved oxygen is more than or equal to 7mg/l；Hatchery should near spawning inducing pool,
Should be furnished with complete water filtering system, build and be relatively suitable in booth, shading, insulation.After fertilization completes 10h, due to embryo
Fetal hair (Crinis Carbonisatus) is educated and is carried out it is desirable to spawning inducing pool current are unsuitable excessive.
This step is all carried out in the environment of lucifuge is sheltered from heat or light.
F, fish roe 80h in hatchery got final product incubation of membrane, and the fry after membrane is in the interior vegetative phase, through 2-4 days
Absorption, fry floats flat trip.This when just can feed the bait of less egg yolk etc, and gets out the standard on the pool under fry
Standby work, can manage fry by the way of net cage hanging culture.
G, the fry obtaining in step f is thrown in, thrown in density: 90000 tails/mu to 110000 tails/mu, preferably
100000 tails/mu；Release time: morning or dusk, do not have direct light to shine, throw in the water temperature temperature difference and be less than or equal to 2 DEG C, throw in
Wheel animalcule quantity in pond water peaks the phase；Throw in pond to require: depth of water 60cm-80cm, dissolved oxygen is more than or equal to 5mg/l,
Enter the configurating filtered facility of discharge outlet, in pond, configure aerator；Fed after input.Throw in pond water quality requirement alive, tender, fertile, refreshing.
Enter discharge outlet should configurating filtered facility, prevent large-scale wild fisheses and cladocera etc. harm fry safety.Before fry feed
Stage tries one's best based on the feedstuff of high nutritive value, and fry ingests a part, and remaining a part of rich water is conducive to fry to live bait
The supply of material.The nursery nurturing period is it should conscientiously pay close attention to Changes in weather, and nursery pond dissolved oxygen situation, and nursery pond is equipped with emergent increasing
Oxygen machine, to prevent fry anoxia.Conscientiously carry out daily water temperature record and management notes, should adhere to daily more than 3 times patrolling the pool, and
When clean out the debris in pond.
H, control and prevention of disease
Onychostoma simus hemorrhagic disease, disease: the body surface fish gill is intact, and whole body is rubescent, particularly fin ray base portion, anus is red and swollen, cuts body cavity open,
Intestinal takes on a red color.Therapeutic scheme: florfenicol, SANHUANGFEN, vitamin k 3 and binding agent are mixed bait and feeds.
Ichthyophthiriasiss, Ichthyophthirius protozoa, Ciliata, recess section, Ichthyophthirius, main parasitic is in Fish
The positions such as skin, fin, the gill, head, oral cavity and eye, the white small point-like of variance in form, visually visible.When serious, fish body is visible from head to foot
Small particles, therefore claim ichthyophthiriasises.It causes body surface respectively to organize hyperemia, can not look for food after the little melonworm of infection of marine fishes, in addition secondary bacterial,
Virus infection, can cause large quantities of fishes dead, its mortality rate is up to 60%～70%, or even is annihilated, and breeding production is brought sternly
Threaten again.Therapeutic Method: the copper sulfate being 0.7ppm with concentration and ferrous sulfate mixed solution full pool spilling head.Should observe at ordinary times
Prevent overweighting the principle controlled.Remove the excessive mud of at the bottom of pond, cement pool wall will be scrubbed, and is disappeared with quick lime or bleaching powder
Poison.For pond, seedling rearing room Cultivated water, increase quantity of exchanged water, improve water quality, periodically sterilize.Seedling rearing room apparatus is through conventional Fu Er
Malin or potassium permanganate solution carry out disinfection, and accomplish that special pond is special.Should be sampled checking before the pool or entrance net cage under fish, if
Find that Ichthyophthirius multifiliis should adopt said method to treat in time.Breeding density is unsuitable excessive, daily should strengthen nutrition, improve fish body
Resistance.
Anchor head pulicosis: when the quantity of lernaean is few, removed with tweezers；When as more in quantity, using potassium permanganate solution
Embathe disease fish.Additionally, when water temperature is 15 DEG C to 20 DEG C, embathing 1-2 hour with the concentration of 10-20ppm, washing 1 time daily, 3 days
Lernaean is all dead afterwards.If water temperature is more than 30 DEG C, concentration to be reduced.Prevention and controls: 1. cleaned up the pond with quick lime band water, consumption
For 200～250 mg/litre.Potassium permanganate liquid dipping bath is used, consumption is 10～20 mg/litre, the time 15～30 during fingerling stocking
Minute.In this worm mating period, full pool spilling head mass concentration is 90% metrifonate, and consumption is 0.5 mg/litre.1 time every two weeks,
It is used in conjunction 2～3 times.
Due to the scarcity of Wild fish resources, severe along with field survivorship environment, the raun typically collected is ripe
Degree is not high, and milter Maturity is good, but individual little, in order to preferably be hastened parturition, in step d, good to Maturity
Plant fish and inject an odinagogue, odinagogue adopts lrh-a2, hcg and dom, the injection volume of every kilogram of raun is the lrh-a2+ of 5ug
The dom of the hcg+2mg of 1000iu, the injection volume of every kilogram of milter halves compared with raun；
To imperfect kind of fish injection odinagogue twice of Maturity, inject for the first time: every kilogram of raun injects the lrh-a2 of 10ug,
The injection volume of every kilogram of milter halves compared with raun；Inject for second: the hcg of the lrh-a2+1000iu of every kilogram of raun injection 5ug
The dom of+3mg, the injection volume of every kilogram of milter halves compared with raun；The time interval of double injection 12 hours.Lrh-a2, hcg and
Dom is existing conventional odinagogue.
In step g, fed as bait using eel powder, bean milk, milk or egg yolk, every ten thousand tail fries feed every time
Bait 5g, feeds 6-8 time daily, it then follows throw multiple principle less.The protein content that eel powder, bean milk, milk or egg yolk are
Height, is conducive to the growth of fry.
After step e1, milter and raun are invaded bubble with the penicillin of 400 units, kind of a fish is carried out disinfection, more separately puts into
Recuperate in postpartum care pond.
Embodiment:
13 kilograms of the vacricorhinus gerlachi that fisherman salvages is bought in certain fish farm, puts into kind of fishpond and feeds.Plant the length that fishpond is 30m*5m
Square pond, depth of water 80cm, 17 DEG C to 22 DEG C of water temperature, dissolved oxygen 6mg/l, discharge meets water per hour and exchanges 2 times.To breeding
In season, meet 5 kilograms of the kind fish that seed selection requires, the kind fish meeting requirement is put into spawning inducing pool and feeds, spawning inducing pool floor space 4
, water exchanges 2.5 times discharge satisfaction per hour, 17 DEG C to 22 DEG C of water temperature, dissolved oxygen 6mg/l；Maturity according to kind of fish to
Kind fish injection odinagogue in spawning inducing pool.When kind of a fish starts mutually to chase, milter is pulled out, dry the water on gonopore
Point, then press abdominal part, allow its seminal fluid to flow out, with syringe by seminal fluid collecting；After treating seminal fluid collecting well, then raun is pulled out,
With towel, the moisture of body is wiped, then press abdominal part, fish roe is clamp-oned in china bowl；Seminal fluid is clamp-oned china bowl, every milter
Seminal fluid mix with the fish roe of 2 rauns；Pour the normal saline 2ml that mass concentration is 0.9% again into, with chicken feather stirring, mixing is all
After even, add clear water, add the ovum of clear water to start to be fertilized, imbibition, continues filling clear water.2min is placed in china bowl
Afterwards, then move in big basin, after fish roe absorbs water fertilization 1h in big basin, germ cell is evenly coated in the artificial fish nest in hatchery
On, and be passed through flowing water into hatchery and hatched, 17 DEG C -22 DEG C of the water temperature of hatchery, dissolved oxygen 6mg/l, after eyed eggs, dissolved oxygen
8mg/l.Incubation of membrane after fish roe 80h in hatchery, the fry after membrane is in the interior vegetative phase, through the absorption of 2-4 days,
Fry floats and puts down trip.Fry is thrown in, is thrown in density: 100000 tails/mu；Release time: morning or dusk, not straight
Penetrate illumination, throw in the water temperature temperature difference and be less than or equal to 2 DEG C, the wheel animalcule quantity thrown in pond water peaks the phase；Throwing in pond will
Ask: depth of water 70cm, dissolved oxygen 6mg/l.Fed as bait using eel powder, bean milk, milk or egg yolk, every ten thousand tail fries are every
Secondary feed bait 5g, feed 7 times daily.The survival rate of fry 50 percent, forms big specification fry 35000 tail.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, made any repair
Change, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (5)
1. a kind of offspring breeding method of vacricorhinus gerlachi is it is characterised in that comprise the following steps:
A, fish will be planted put into kind of fishpond and feed, kinds fishpond will be rectangle pond, depth of water 60cm to 100cm, 17 DEG C to 22 DEG C of water temperature,
Dissolved oxygen is more than or equal to 5mg/l, and discharge meets water per hour and exchanges 2 times；
B, to mating period when select kind of a fish: on milter mouth pearl star substantially, appearance not damaged, no deformity, light pressure abdominal part can flow out
Milky seminal fluid, the long 12cm to 18cm of body, body weight 120g to 180g；Raun abdominal part expands, soft, body surface not damaged, and scale is complete
Whole, gonopore pinkiness, the long 17cm to 24cm of body, body weight is more than or equal to 200g；
C, the kind fish picking out step b are put into spawning inducing pool and feed, spawning inducing pool floor space 3 to 4, and discharge meets often little
Shi Chishui at least exchanges 2 times, 17 DEG C to 22 DEG C of water temperature, and dissolved oxygen is more than or equal to 5mg/l；
D, in spawning inducing pool kind fish injection odinagogue；
E, artificial incubation:
E1, when kind of a fish starts mutually to chase, milter is pulled out, dries the moisture on gonopore, then press abdominal part, allow it
Seminal fluid flows out, with syringe by seminal fluid collecting；After treating seminal fluid collecting well, then raun is pulled out, with towel by the moisture of body
Wipe, then press abdominal part, fish roe is clamp-oned in china bowl；
E2, seminal fluid is clamp-oned china bowl, the seminal fluid that the ratio of seminal fluid and fish roe meets 1 milter is mixed with the fish roe of 2 to 3 rauns
Close；Pour the normal saline 2ml that mass concentration is 0.9% again into, stirring, after mix homogeneously, add clear water, from being stirred to addition
Clear water is less than 30 seconds interval time, quick and graceful in the movements, the tenderness of stirring, adds the ovum of clear water to start to be fertilized, and imbibition continues
Filling clear water；
After e3, placement 1min to 2min in china bowl, then move in big basin, after fish roe absorbs water fertilization 1h in big basin, will be subject to
Smart ovum is evenly coated on the artificial fish nest in hatchery, and is passed through flowing water into hatchery and is hatched, the water temperature 17 of hatchery
DEG C -22 DEG C, dissolved oxygen is more than or equal to 5mg/l, and after eyed eggs, dissolved oxygen is more than or equal to 7mg/l；
This step is all carried out in the environment of lucifuge is sheltered from heat or light；
F, fish roe 80h in hatchery got final product incubation of membrane, and the fry after membrane is in the interior vegetative phase, through the suction of 2-4 days
Receive, fry floats and puts down trip；
G, the fry obtaining in step f is thrown in, thrown in density: 90000 tails/mu to 110000 tails/mu；Release time:
Morning or dusk, do not have direct light to shine, throw in the water temperature temperature difference and be less than or equal to 2 DEG C, the wheel animalcule quantity thrown in pond water reaches
To peak period；Throw in pond to require: depth of water 60cm-80cm, dissolved oxygen is more than or equal to 5mg/l, enters the configurating filtered facility of discharge outlet,
Aerator is configured in pond；Fed after input；
H, control and prevention of disease.
2. a kind of offspring breeding method of vacricorhinus gerlachi according to claim 1 is it is characterised in that in step d, to one-tenth
The ripe kind fish spent injects an odinagogue, and odinagogue adopts lrh-a2, hcg and dom, and the injection volume of every kilogram of raun is 5ug
The hcg+2mg of lrh-a2+1000iu dom, the injection volume of every kilogram of milter halves compared with raun；
To imperfect kind of fish injection odinagogue twice of Maturity, inject for the first time: every kilogram of raun injects the lrh-a2 of 10ug,
The injection volume of every kilogram of milter halves compared with raun；Inject for second: the hcg of the lrh-a2+1000iu of every kilogram of raun injection 5ug
The dom of+3mg, the injection volume of every kilogram of milter halves compared with raun；The time interval of double injection 12 hours.
3. a kind of offspring breeding method of vacricorhinus gerlachi according to claim 1 is it is characterised in that in step g, adopt
Eel powder, bean milk, milk or egg yolk are fed as bait, and every ten thousand tail fries feed bait 5g every time, feed 6-8 daily
Secondary, it then follows to throw multiple principle less.
4. a kind of offspring breeding method of vacricorhinus gerlachi according to claim 1 is it is characterised in that after step e1, incite somebody to action
Milter and raun are soaked with the penicillin of 400 units, more separately put into the recuperation of postpartum care pond.
5. a kind of offspring breeding method of vacricorhinus gerlachi according to claim 1 is it is characterised in that in step h, including
The prevention and control of onychostoma simus hemorrhagic disease, ichthyophthiriasiss and anchor head pulicosis,
Onychostoma simus hemorrhagic disease: florfenicol, SANHUANGFEN, vitamin k 3 and binding agent are mixed bait and feeds；
Ichthyophthiriasiss: with copper sulfate and ferrous sulfate mixed solution full pool spilling head；
Anchor head pulicosis: when the quantity of lernaean is few, removed with tweezers；When as more in quantity, embathed using potassium permanganate solution
Sick fish.
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| CN112293411A (en) * | 2020-11-27 | 2021-02-02 | 大连海洋大学 | Low-temperature preservation liquid and preservation method for sperms of echinococcus intermedius |
| CN112616729A (en) * | 2020-12-30 | 2021-04-09 | 铜仁市渔业技术推广站 | Artificial propagation method of small-mouth white soft-shelled turtles |
| CN112616730A (en) * | 2020-12-30 | 2021-04-09 | 铜仁市渔业技术推广站 | Method for cultivating small-mouth white soft-shelled turtle seedlings |
| CN112616731A (en) * | 2020-12-30 | 2021-04-09 | 铜仁市渔业技术推广站 | Small white soft-shelled turtle bionic breeding pond and breeding method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110384059A (en) * | 2019-09-06 | 2019-10-29 | 西南大学 | A kind of control method of fresh-water fishes ich |
| CN112293411A (en) * | 2020-11-27 | 2021-02-02 | 大连海洋大学 | Low-temperature preservation liquid and preservation method for sperms of echinococcus intermedius |
| CN112616729A (en) * | 2020-12-30 | 2021-04-09 | 铜仁市渔业技术推广站 | Artificial propagation method of small-mouth white soft-shelled turtles |
| CN112616730A (en) * | 2020-12-30 | 2021-04-09 | 铜仁市渔业技术推广站 | Method for cultivating small-mouth white soft-shelled turtle seedlings |
| CN112616731A (en) * | 2020-12-30 | 2021-04-09 | 铜仁市渔业技术推广站 | Small white soft-shelled turtle bionic breeding pond and breeding method |
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