CN106344630B - A kind of antiphlogistic and analgesic ointment and preparation method thereof containing chlorophyll - Google Patents
A kind of antiphlogistic and analgesic ointment and preparation method thereof containing chlorophyll Download PDFInfo
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Abstract
The present invention relates to a kind of antiphlogistic and analgesic ointment and preparation method thereof containing chlorophyll.The antiphlogistic and analgesic ointment containing chlorophyll is made of the following raw material for preparing: active constituent, stabilizer and matrix, wherein active constituent is made of the following raw material for preparing: chlorophyll, propolis extract, oblongleaf garcinia seed oil and the compound of glauber-salt and liquorice；Matrix is made of the following raw material for preparing: lanolin, crambe abyssinica seed oil, albolene, cetanol, isopropyl myristate, glycerol, polyglyceryl fatty acid ester, ethyl-para-hydroxybenzoate, surplus are purified water；Stabilizer is butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with (1~2): the weight ratio of (0.2~0.8) forms.Antiphlogistic and analgesic ointment of the present invention containing chlorophyll is safe and effective, to no skin irritation, will not cause allergic reaction.
Description
Technical field
The present invention relates to a kind of external preparations, and in particular to a kind of to contain the antiphlogistic and analgesic ointment of chlorophyll and its preparation side
Method.
Background technique
Antiphlogistic and analgesic ointment is the external patch topical application of drug, is mainly aided with common matrix by Chinese and Western pharmaceutically active ingredient and is prepared, tool
There are blood circulation and removing stasis, profit gas dissipating bind, clearing heat and detoxicating, swelling and pain relieving, cures mainly various arthralgias.Wherein common Chinese medicine at
Point there are olibanum, myrrh, blood to have a rest, Radix Angelicae Pubescentis, rheum officinale, catechu, saltcake, borneol etc., common western medicine composition has gaultherolin, to second
Acylamino- phenol, Indomethacin etc..Since antiphlogistic and analgesic ointment directly applies on Lesional Skin, also brought while working rapid
Some security risks, such as cause to stimulate to skin, easily cause allergic reaction.
Chlorophyll is that photosynthetic important pigment is participated in green plants chloroplaset, and structure and ferroheme are extremely similar,
It is easily absorbed by the body, plays the role of assigning living cells, have extensive use in medicine, food and daily chemical industry.At present it has proven convenient that
Chlorophyll has the function of to improve constipation, reduce cholesterol, anti-aging, toxin expelling anti-inflammatory, deodorization, anticancer, antimutation, anti-anemia action etc.,
There is palingenesis to skin histology, can be used for treating scald, chronic ulcer can inhibit staphylococcus and streptococcus growth, to tooth
Oulitis, halitosis, tympanitis etc. have certain curative effect.
Chinese patent application 201310295240.4 discloses a kind of activating microcirculation and removing stasis medicinal, antiphlogistic and analgesic ointment and preparation method thereof,
The activating microcirculation and removing stasis medicinal, antiphlogistic and analgesic ointment are made of pharmaceutical composition and matrix, wherein pharmaceutical composition is by Chinese medicine composition, celestial being
Slap extract and gaultherolin composition；Chinese medicine composition is made of following bulk pharmaceutical chemicals: Radix Angelicae Sinensis, radix paeoniae rubra, olibanum, does not have peach kernel
Medicine, pheretima, dragon's blood, Radix Notoginseng, monkshood and borneol, which effectively combines Chinese medicine composition and Western medicine, for living
Blood stagnation resolvation and anti-inflammatory analgetic.
Summary of the invention
In order to solve the problems existing in the prior art, the present invention provides a kind of antiphlogistic and analgesic ointment containing chlorophyll and its preparation
Method.Antiphlogistic and analgesic ointment of the present invention containing chlorophyll is safe and effective, to no skin irritation, allergy will not be caused anti-
It answers.
The present invention is by following technical solution to be attained in that
A kind of antiphlogistic and analgesic ointment containing chlorophyll prepares raw material by following weight percent meter and forms: active constituent 15
~25%, stabilizer 2~8% and matrix 70~80%；
Wherein, the active constituent is made of the raw material for preparing of following weight percent meter: chlorophyll 20~40%, propolis
Extract 20~30%, oblongleaf garcinia seed oil 20~30% and the compound of glauber-salt and liquorice 10~30%；
The matrix is made of the raw material for preparing of following weight percent meter: lanolin 5~15%, crambe abyssinica seed oil 1~
6%, albolene 10~20%, cetanol 5~10%, isopropyl myristate 4~8%, glycerol 10~20%, polyglycerol ester
Fat acid esters 4~8%, ethyl-para-hydroxybenzoate 0.1~0.3%, surplus are purified water；
The stabilizer is butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with (1~2): (0.2
~0.8) weight ratio composition.
Preferably, the antiphlogistic and analgesic ointment prepares raw material by following weight percent meter and forms: active constituent 25%, steady
Determine agent 5% and matrix 70%；
Preferably, the active constituent is made of the raw material for preparing of following weight percent meter: chlorophyll 30%, propolis mention
Take object 25%, oblongleaf garcinia seed oil 25% and the compound of glauber-salt and liquorice 20%；
Preferably, the matrix is made of the raw material for preparing of following weight percent meter: lanolin 12%, crambe abyssinica seed oil
4%, albolene 16%, cetanol 8%, isopropyl myristate 6%, glycerol 15%, polyglyceryl fatty acid ester 8%, to hydroxyl
Yl benzoic acid ethyl ester 0.2%, surplus are purified water；
Preferably, the stabilizer be butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with (1~
1.5): the weight ratio composition of (0.4~0.8).
It is highly preferred that the stabilizer is butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with 1:
0.5 weight ratio composition.
Further, the preparation method of the propolis extract includes the following steps:
(1) propolis paraffin removal is placed on 2~4h of pre-freeze in -10 DEG C of refrigerator, crushed, crossed 30 meshes, obtain propolis powder；
(2) ethanol solution that volume fraction is 95% is added into propolis powder for the ratio for being 1:12 in solid-liquid ratio, stirring
Sealing after uniformly impregnates 8~12min at 25 DEG C under conditions of 150~250MPa, after release, under room temperature, standing 2~
4h obtains propolis extract；
(3) propolis extract is placed in -50 DEG C of pre-freeze 3h, is then 50~60Pa in vacuum degree, is lyophilized 6 under the conditions of -5 DEG C
~8h keeps vacuum degree, continues 1~2h of freeze-drying at 20 DEG C to get propolis extract.
Further, the preparation method of the oblongleaf garcinia seed oil includes the following steps: to take garcinia multiflora seed, cleans, and drying is gone
Garcinia multiflora seed benevolence is crushed into powder shape by shell, crosses 50 meshes, is subsequently placed in supercritical extraction reactor and carries out 1~2h of extraction, CO is arranged2
Flow be 15~25L/h, extracting pressure be 30~40Ma, extraction temperature be 35~40 DEG C.
In addition, the present invention also provides the preparation method of the antiphlogistic and analgesic ointment containing chlorophyll described in one kind, including it is following
Step:
(1) chlorophyll is taken, the dehydrated alcohol that its weight 50% is added is dissolved, spare；
(2) oblongleaf garcinia seed oil, crambe abyssinica seed oil, lanolin, albolene, cetanol, isopropyl myristate, polyglycereol are taken
Aliphatic ester and butylated hydroxyarisol mix, and heating water bath heats while stirring to 80 DEG C, until material is completely molten
Solution obtains oily phase, keeps the temperature spare；
(3) compound of glauber-salt and liquorice, 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium, glycerol and purified water is taken to mix, heating water bath to 80 DEG C,
It heats while stirring, until material is completely dissolved, obtains water phase, keep the temperature spare；
(4) water phase is added in oily phase, 80 DEG C of insulated and stirred 5~10min, it is to be emulsified completely after, be cooled to 40 DEG C, so
After dissolved chlorophyll, propolis extract and ethyl-para-hydroxybenzoate is added, continue stirring to room temperature to get.
Further, for ease of use, antiphlogistic and analgesic ointment of the present invention can be further made to patch, every patch contains
Cream amount is 1~2g.
Chlorophyll is magnesium porphyrin compound, and structure is extremely similar to ferroheme, is easily absorbed by the body, and has and assigns living cells
Effect promotes regeneration, antibacterial anti-inflammatory；Propolis extract not only has effects that moisturizing myogenic, can also enhance chlorophyll
Anti-inflammatory analgetic effect, and cutin-softening tissue, promote other effective components such as chlorophyll to penetrate into skin, promote to absorb；Wood bamboo
Seed oil not only has effects that it is green to be conducive to alleviation leaf for anti-inflammatory analgetic, convergence myogenic, a large amount of unsaturated fatty acid contained
The oxygenolysis of element, improves the stability of chlorophyll；The main component of the compound of glauber-salt and liquorice be sodium sulphate, have it is clearing heat and detoxicating, Li Shui disappears
Swollen effect can enhance anti-inflammatory analgetic effect of chlorophyll, in addition, it contains the ingredients such as zinc sulfate, calcium sulfate toward contact, it is described
Zinc ion and chlorophyll formation complex compound, be conducive to improve chlorophyll stability.
Compared with prior art, present invention has an advantage that
(1) each active constituent is mutually coordinated in antiphlogistic and analgesic ointment of the present invention, mutually promotes, and has and preferably disappears
Scorching, analgesic efficacy, and it is safe and reliable, to no skin irritation, will not cause allergic reaction.
(2) antiphlogistic and analgesic ointment addition butylated hydroxyarisol and 2-Phenylbenzimidazole -5- sulphur of the present invention
The mixture of sour sodium is conducive to improve stability of the chlorophyll in preparation, storage and use process, enhancing is treated as stabilizer
Effect.
(3) antiphlogistic and analgesic ointment property of the present invention is stablized, and is easy to be coated with, curative for effect.
Specific embodiment
The present invention is further described below by way of specific embodiment, but the present invention is not limited only to following embodiment.
The preparation of 1 propolis extract of embodiment
Test the preparation of A group propolis extract:
(1) propolis paraffin removal is placed on pre-freeze 3h in -10 DEG C of refrigerator, crushed, crossed 30 meshes, obtain propolis powder；
(2) ethanol solution that volume fraction is 95% is added into propolis powder for the ratio for being 1:12 in solid-liquid ratio, stirring
Sealing, at 25 DEG C, impregnates 10min after uniformly under conditions of 200MPa, after release, under room temperature, stands 3h, obtains propolis extraction
Liquid；
(3) propolis extract is placed in -50 DEG C of pre-freeze 3h, is then 50Pa in vacuum degree, 8h is lyophilized under the conditions of -5 DEG C, protects
Vacuum degree is held, continues to be lyophilized 2h at 20 DEG C to get propolis extract.
The preparation method for testing B, C group propolis extract is essentially identical with test A group, and difference is dipping in step (2)
Pressure be respectively 250MPa and 150MPa.
The rate of extract (%) and general flavone content (%) of propolis extract made from measurement test A, B, C group respectively, as a result
It see the table below.
| Testing index | A group | B group | C group |
| The rate of extract (%) | 49.87 | 48.26 | 46.13 |
| General flavone content (%) | 57.38 | 55.87 | 54.38 |
As seen from the above table, the rate of extract and general flavone content highest of propolis extract made from test A group, therefore to test A
The conduct of propolis extract made from organizing prepares raw material and prepares antiphlogistic and analgesic ointment of the present invention.
The preparation of 2 oblongleaf garcinia seed oil of embodiment
Preparation method: taking garcinia multiflora seed, cleans, drying, and garcinia multiflora seed benevolence is crushed into powder shape by decladding, crosses 50 meshes,
It is subsequently placed in supercritical extraction reactor and carries out extraction 1.5h, CO is set2Flow be 25L/h, extracting pressure 35Ma, extraction temperature
It is 40 DEG C.
The oil yield of the oblongleaf garcinia seed oil is 92.5%.
The preparation of 3 antiphlogistic and analgesic ointment of embodiment
Antiphlogistic and analgesic ointment described in the embodiment of the present invention 3 prepares raw material by following weight percent meter and forms: active constituent
25%, stabilizer 5% and matrix 70%；
Wherein, the active constituent is made of the raw material for preparing of following weight percent meter: chlorophyll 30%, propolis extract
Object 25%, oblongleaf garcinia seed oil 25% and the compound of glauber-salt and liquorice 20%；
The matrix is made of the raw material for preparing of following weight percent meter: lanolin 12%, crambe abyssinica seed oil 4%, white
Vaseline 16%, cetanol 8%, isopropyl myristate 6%, glycerol 15%, polyglyceryl fatty acid ester 8%, para hydroxybenzene first
Acetoacetic ester 0.2%, surplus are purified water；
The stabilizer is butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with the weight of 1:0.5
Than composition.
Preparation method:
(1) chlorophyll is taken, the dehydrated alcohol that its weight 50% is added is dissolved, spare；
(2) oblongleaf garcinia seed oil, crambe abyssinica seed oil, lanolin, albolene, cetanol, isopropyl myristate, polyglycereol are taken
Aliphatic ester and butylated hydroxyarisol mix, and heating water bath heats while stirring to 80 DEG C, until material is completely molten
Solution obtains oily phase, keeps the temperature spare；
(3) compound of glauber-salt and liquorice, 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium, glycerol and purified water is taken to mix, heating water bath to 80 DEG C,
It heats while stirring, until material is completely dissolved, obtains water phase, keep the temperature spare；
(4) water phase is added in oily phase, 80 DEG C of insulated and stirred 10min, it is to be emulsified completely after, be cooled to 40 DEG C, then
Dissolved chlorophyll, propolis extract and ethyl-para-hydroxybenzoate is added, continues stirring to room temperature to get anti-inflammatory analgetic
Cream；
(4) using modern general preparation technique, patch is made in antiphlogistic and analgesic ointment, every patch paste containing amount is 1g.
The preparation of 4 antiphlogistic and analgesic ointment of embodiment
Antiphlogistic and analgesic ointment described in the embodiment of the present invention 4 prepares raw material by following weight percent meter and forms: active constituent
15%, stabilizer 5% and matrix 80%；
Wherein, the active constituent is made of the raw material for preparing of following weight percent meter: chlorophyll 40%, propolis extract
Object 20%, oblongleaf garcinia seed oil 20% and the compound of glauber-salt and liquorice 20%；
The matrix is made of the raw material for preparing of following weight percent meter: lanolin 12%, crambe abyssinica seed oil 6%, white
Vaseline 12%, cetanol 10%, isopropyl myristate 8%, glycerol 15%, polyglyceryl fatty acid ester 5%, para hydroxybenzene first
Acetoacetic ester 0.1%, surplus are purified water；
The stabilizer is butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with the weight of 1:0.8
Than composition.
Preparation method is the same as embodiment 3.
The preparation of 5 antiphlogistic and analgesic ointment of embodiment
Antiphlogistic and analgesic ointment described in the embodiment of the present invention 5 prepares raw material by following weight percent meter and forms: active constituent
20%, stabilizer 5% and matrix 75%；
Wherein, the active constituent is made of the raw material for preparing of following weight percent meter: chlorophyll 20%, propolis extract
25%, oblongleaf garcinia seed oil 30% and the compound of glauber-salt and liquorice 25%；
The matrix is made of the raw material for preparing of following weight percent meter: lanolin 10%, crambe abyssinica seed oil 2%, white
Vaseline 18%, cetanol 10%, isopropyl myristate 5%, glycerol 15%, polyglyceryl fatty acid ester 8%, para hydroxybenzene first
Acetoacetic ester 0.3%, surplus are purified water；
The stabilizer is butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with the weight of 1:0.4
Than composition.
Preparation method is the same as embodiment 3.
The preparation of 1 antiphlogistic and analgesic ointment of comparative example
Antiphlogistic and analgesic ointment described in comparative example 1 of the present invention prepares raw material by following weight percent meter and forms: active constituent
25% and matrix 75%；
Wherein, the active constituent is made of the raw material for preparing of following weight percent meter: chlorophyll 30%, propolis extract
Object 25%, oblongleaf garcinia seed oil 25% and the compound of glauber-salt and liquorice 20%；
The matrix is made of the raw material for preparing of following weight percent meter: lanolin 12%, crambe abyssinica seed oil 4%, white
Vaseline 16%, cetanol 8%, isopropyl myristate 6%, glycerol 15%, polyglyceryl fatty acid ester 8%, para hydroxybenzene first
Acetoacetic ester 0.2%, surplus are purified water；
Preparation method reference implementation example 3, essentially identical, the area for preparing raw material of this comparative example and 3 antiphlogistic and analgesic ointment of embodiment
It is not not add stabilizer, and increase accordingly the ratio of matrix.
The preparation of 2 antiphlogistic and analgesic ointment of comparative example
Antiphlogistic and analgesic ointment described in comparative example 2 of the present invention prepares raw material by following weight percent meter and forms: active constituent
25%, stabilizer 5% and matrix 70%；
Wherein, the active constituent is made of the raw material for preparing of following weight percent meter: chlorophyll 30%, propolis extract
Object 25%, oblongleaf garcinia seed oil 25% and the compound of glauber-salt and liquorice 20%；
The matrix is made of the raw material for preparing of following weight percent meter: lanolin 12%, crambe abyssinica seed oil 4%, white
Vaseline 16%, cetanol 8%, isopropyl myristate 6%, glycerol 15%, polyglyceryl fatty acid ester 8%, para hydroxybenzene first
Acetoacetic ester 0.2%, surplus are purified water；
The stabilizer is butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with the weight ratio of 1:1
Composition.
Preparation method reference implementation example 3, essentially identical, the area for preparing raw material of this comparative example and 3 antiphlogistic and analgesic ointment of embodiment
Be not the stabilizer be butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with etc. weight ratios form.
The preparation of 3 antiphlogistic and analgesic ointment of comparative example
Antiphlogistic and analgesic ointment described in comparative example 3 of the present invention prepares raw material by following weight percent meter and forms: active constituent
25%, stabilizer 5% and matrix 70%；
Wherein, the active constituent is made of the raw material for preparing of following weight percent meter: chlorophyll 40%, propolis extract
Object 30% and oblongleaf garcinia seed oil 30%；
The matrix is made of the raw material for preparing of following weight percent meter: lanolin 12%, crambe abyssinica seed oil 4%, white
Vaseline 16%, cetanol 8%, isopropyl myristate 6%, glycerol 15%, polyglyceryl fatty acid ester 8%, para hydroxybenzene first
Acetoacetic ester 0.2%, surplus are purified water；
The stabilizer is butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with the weight of 1:0.5
Than composition.
Preparation method reference implementation example 3, essentially identical, the area for preparing raw material of this comparative example and 3 antiphlogistic and analgesic ointment of embodiment
It is not that the active constituent does not add the compound of glauber-salt and liquorice, and increase accordingly the ratio of other compositions.
The preparation of 4 antiphlogistic and analgesic ointment of comparative example
Antiphlogistic and analgesic ointment described in comparative example 4 of the present invention prepares raw material by following weight percent meter and forms: active constituent
25%, stabilizer 5% and matrix 70%；
Wherein, the active constituent is made of the raw material for preparing of following weight percent meter: chlorophyll 40%, propolis extract
Object 30% and the compound of glauber-salt and liquorice 30%；
The matrix is made of the raw material for preparing of following weight percent meter: lanolin 12%, crambe abyssinica seed oil 4%, white
Vaseline 16%, cetanol 8%, isopropyl myristate 6%, glycerol 15%, polyglyceryl fatty acid ester 8%, para hydroxybenzene first
Acetoacetic ester 0.2%, surplus are purified water；
The stabilizer is butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with the weight of 1:0.5
Than composition.
Preparation method reference implementation example 3, essentially identical, the area for preparing raw material of this comparative example and 3 antiphlogistic and analgesic ointment of embodiment
It is not that the active constituent does not add oblongleaf garcinia seed oil, and increase accordingly the ratio of other compositions.
Test example one, antiphlogistic and analgesic ointment study on the stability of the present invention
Take antiphlogistic and analgesic ointment made from 3-5 of the embodiment of the present invention and comparative example 1-4, respectively carry out centrifugal test, resist cold it is resistance to
Heat test and accelerated test, to evaluate antiphlogistic and analgesic ointment stability, specifically:
Centrifugal test: taking 10g sample to be placed in centrifuge tube, is centrifuged 30min with the revolving speed of 2500rpm/min, observes sample
Whether lamination is occurred；
Cold-resistant heat resistant test: taking 10g sample to be fitted into closed plastic box, places respectively in -15 DEG C of refrigerators for 24 hours, at 55 DEG C
Insulating box places 6h, has seen whether demulsifying phenomenon；
Accelerated test: taking 10g, and being placed in temperature is 30 ± 2 DEG C, under 60 ± 5% environment of relative humidity, respectively 1,2,3,6
A the end of month takes whether sample investigation has phenomena such as rancid, discoloration, layering, and measures the changes of contents of chlorophyll.
Test result is shown in Tables 1 and 2.
The antiphlogistic and analgesic ointment study on the stability of the present invention of table 1
| Group | Centrifugal test | Heat resistant test | Resistance to jelly experiment | Accelerated test |
| Embodiment 3 | Water oil is not stratified | Without demulsification, it is not stratified | Without demulsification, it is not stratified | Without rancid, not stratified |
| Embodiment 4 | Water oil is not stratified | Without demulsification, it is not stratified | Without demulsification, it is not stratified | Without rancid, not stratified |
| Embodiment 5 | Water oil is not stratified | Without demulsification, it is not stratified | Without demulsification, it is not stratified | Without rancid, not stratified |
| Comparative example 1 | Water oil is not stratified | Without demulsification, it is not stratified | Without demulsification, it is not stratified | Rancid, layering |
| Comparative example 2 | Water oil is not stratified | Without demulsification, it is not stratified | Without demulsification, it is not stratified | Rancid, layering |
| Comparative example 3 | Water oil is not stratified | Without demulsification, it is not stratified | Without demulsification, it is not stratified | Without rancid, not stratified |
| Comparative example 4 | Water oil is not stratified | There are demulsification, permeability | There are demulsification, permeability | Without rancid, not stratified |
The antiphlogistic and analgesic ointment of the present invention of table 2 is investigated in accelerated test Determination of Chlorophyll changes of contents
The results show that antiphlogistic and analgesic ointment made from embodiment 3-5 is in centrifugal test, cold-resistant heat resistant test and accelerates examination
It tests and preferable stability is presented, demulsification, water oil layer and rancid phenomenon do not occur, and in accelerated test Determination of Chlorophyll content
Reduction is not significant, shows that the antiphlogistic and analgesic ointment property of the present invention containing chlorophyll is stablized.
By comparative example 1 and comparative example 2 it is found that do not add butylated hydroxyarisol in stabilizer or stabilizer and
2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with etc. weight ratios mixing, antiphlogistic and analgesic ointment obtained occur in accelerated test it is rancid, point
Layer phenomenon, and chlorophyll content reduction is more significant, is especially dropped with antiphlogistic and analgesic ointment Determination of Chlorophyll content made from comparative example 1
It is low the most significant, show that chlorophyll point can be effectively suppressed in butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium
Solution is used as stabilizer when the two mixes in the proper ratio, can significantly increase the stability of antiphlogistic and analgesic ointment, inhibits rancid, divides
Layer occurs.
By comparative example 3 and comparative example 4 it is found that the compound of glauber-salt and liquorice does not influence the appearance stability of antiphlogistic and analgesic ointment, but can promote leaf
Green element decomposes；Oblongleaf garcinia seed oil not only influences the appearance stability of antiphlogistic and analgesic ointment, may also suppress chlorophyll decomposition, when shortage wood bamboo
Seed oil can lead to antiphlogistic and analgesic ointment and have demulsification, permeability phenomenon, and chlorophyll decomposes and accelerates.
Test example two, antiphlogistic and analgesic ointment safety detection of the present invention
Anti-inflammatory made from embodiment 3-5 and comparative example 1-4 is stopped according to the requirement of " new drug toxicologic study guideline "
Pain cream carries out skin irritation, anaphylaxis evaluation, the results are shown in Table 3.
The antiphlogistic and analgesic ointment safety detection result of the present invention of table 3
| Group | Phenomenon | Conclusion |
| Embodiment 3 | Without erythema, without oedema | It is non-stimulated, without sensitization |
| Embodiment 4 | Without erythema, without oedema | It is non-stimulated, without sensitization |
| Embodiment 5 | Without erythema, without oedema | It is non-stimulated, without sensitization |
| Comparative example 1 | Without erythema, without oedema | It is non-stimulated, without sensitization |
| Comparative example 2 | Without erythema, without oedema | It is non-stimulated, without sensitization |
| Comparative example 3 | Without erythema, without oedema | It is non-stimulated, without sensitization |
| Comparative example 4 | Without erythema, without oedema | It is non-stimulated, without sensitization |
The results show that antiphlogistic and analgesic ointment made from 3-5 of the embodiment of the present invention and comparative example 1-4 is non-stimulated to skin, it will not
It causes allergic reaction, it is highly-safe.
Test example three, antiphlogistic and analgesic ointment of the present invention are to the evaluating drug effect of rheumatoid arthritis
The volunteer patients that 480 clinical definites are rheumatoid arthritis are screened, 6 groups is randomly divided into, every group 80, distinguishes
For positive controls, 3 groups of embodiment, comparative example 1-4 group.Wherein, positive controls volunteer patients use commercially available antiphlogistic and analgesic ointment
(Baiyunshan Pharmaceutics Stock-sharing Co., Ltd., Guangzhou City), 1~2 patch/joint, is subject to and covers partial gall area every time, for 24 hours rear dressing
1 time, 3 groups of embodiment and comparative example 1-4 group volunteer patients use antiphlogistic and analgesic ointment made from embodiment 3 and comparative example 1-4 respectively,
Administration mode same positive controls after each group continuous use 7 days, are deactivated 1 day, then are carried out after being used in conjunction 7 days using digit score method
(VAS) measurement pain scores variation, and evaluate arthritis improvement situation, wherein VAS pain scores standard are as follows: 0 point: painless；
3 points or less: having slight pain, can endure；4~6 points: patient pain simultaneously influences to sleep, and can still endure；7~10 points: Huan Zheyou
The pain of crescendo, it is in great pain, influence appetite and sleep.
It the results are shown in Table 4.
The antiphlogistic and analgesic ointment of the present invention of table 4 is to rheumatoid arthritis treatment effect
Note: compared with before treatment,*P < 0.05,**P<0.01；Compared with 3 groups of embodiment,#P < 0.05, with positive controls
Compare,△P<0.05
Commercially available antiphlogistic and analgesic ointment contain simultaneously in, western the effective elements of the medicine, specially menthol, camphor, borneol, thyme
Brain, belladonna liquid extract, bagodryl hydrochloride, gaultherolin have preferable anti-inflammatory analgetic effect, are mainly used for treatment nerve
Bitterly, rheumatalgia, shoulder pain are sprained, and arthralgia, myalgia, the present invention is using commercially available antiphlogistic and analgesic ointment as positive control drug to comment
Therapeutic effect of the antiphlogistic and analgesic ointment containing chlorophyll made from the valence embodiment of the present invention 3 to rheumatoid arthritis.
The results show that antiphlogistic and analgesic ointment made from embodiment 3 can significantly reduce the VAS of rheumatoid arthritis volunteer patients
Pain scores and arthritis total mark (P < 0.01), effect is suitable with the therapeutic effect of commercially available antiphlogistic and analgesic ointment, comparative example 2
Antiphlogistic and analgesic ointment obtained still has certain curative effect to the pain symptom for alleviating volunteer patients, but improves patient articular's inflammation effect
Fruit is not significant, and antiphlogistic and analgesic ointment made from comparative example 1,3 and 4 is equal to the pain symptom and arthritis effect of alleviating volunteer patients
It is not significant.
During test, volunteer patients have no any using antiphlogistic and analgesic ointment made from the embodiment of the present invention 3 and comparative example 1-4
Adverse reaction, but commercially available antiphlogistic and analgesic ointment has 2 volunteer patients local fash occur, shows anti-inflammatory analgetic provided by the invention
Cream safety is good.
The above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
Limitation of the invention, protection scope of the present invention should be defined by the scope defined by the claims..For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
It also should be regarded as protection scope of the present invention into retouching.
Claims (6)
1. a kind of antiphlogistic and analgesic ointment containing chlorophyll, which is characterized in that the antiphlogistic and analgesic ointment is by following weight percent
Meter prepares raw material composition: active constituent 15~25%, stabilizer 2~8% and matrix 70~80%；
The active constituent is made of the raw material for preparing of following weight percent meter: chlorophyll 20~40%, propolis extract 20
~30%, oblongleaf garcinia seed oil 20~30% and the compound of glauber-salt and liquorice 10~30%；
The matrix is made of the raw material for preparing of following weight percent meter: lanolin 5~15%, crambe abyssinica seed oil 1~6%,
Albolene 10~20%, cetanol 5~10%, isopropyl myristate 4~8%, glycerol 10~20%, polyglycerol fatty acid
Ester 4~8%, ethyl-para-hydroxybenzoate 0.1~0.3%, surplus are purified water；
The stabilizer is butylated hydroxyarisol and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium with (1~1.5): (0.4~
0.8) weight ratio composition；
The preparation method of the propolis extract includes the following steps:
(1) propolis paraffin removal is placed on 2~4h of pre-freeze in -10 DEG C of refrigerator, crushed, crossed 30 meshes, obtain propolis powder；
(2) ethanol solution that volume fraction is 95% is added into propolis powder for the ratio for being 1:12 in solid-liquid ratio, stirs evenly
After seal, at 25 DEG C, under conditions of 150~250MPa impregnate 8~12min, after release, under room temperature, stand 2~4h, obtain
Propolis extract；
(3) propolis extract is placed in -50 DEG C of pre-freeze 3h, is then 50~60Pa in vacuum degree, freeze-drying 6 under the conditions of -5 DEG C~
8h keeps vacuum degree, continues 1~2h of freeze-drying at 20 DEG C to get propolis extract.
2. the antiphlogistic and analgesic ointment according to claim 1 containing chlorophyll, which is characterized in that the antiphlogistic and analgesic ointment by
Following weight percent meter prepares raw material composition: active constituent 25%, stabilizer 5% and matrix 70%；
The active constituent is made of the raw material for preparing of following weight percent meter: chlorophyll 30%, propolis extract 25%, wood
Bamboo oil 25% and the compound of glauber-salt and liquorice 20%；
The matrix is made of the raw material for preparing of following weight percent meter: lanolin 12%, crambe abyssinica seed oil 4%, Bai Fanshi
Woods 16%, cetanol 8%, isopropyl myristate 6%, glycerol 15%, polyglyceryl fatty acid ester 8%, P-hydroxybenzoic acid second
Ester 0.2%, surplus are purified water.
3. the antiphlogistic and analgesic ointment according to claim 1 containing chlorophyll, which is characterized in that the stabilizer is tert-butyl
P-hydroxyanisole and 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium are formed with the weight ratio of 1:0.5.
4. the antiphlogistic and analgesic ointment according to claim 1 or 2 containing chlorophyll, which is characterized in that the oblongleaf garcinia seed oil
Preparation method includes the following steps: to take garcinia multiflora seed, cleans, drying, decladding, and garcinia multiflora seed benevolence is crushed into powder shape, crosses 50
Mesh is subsequently placed in supercritical extraction reactor and carries out 1~2h of extraction, CO is arranged2Flow be 15~25L/h, extracting pressure 30
~40MPa, extraction temperature are 35~40 DEG C.
5. a kind of method for preparing the antiphlogistic and analgesic ointment containing chlorophyll as described in Claims 1 to 4 is any, feature exist
In, comprising the following steps:
(1) chlorophyll is taken, the dehydrated alcohol that its weight 50% is added is dissolved, spare；
(2) oblongleaf garcinia seed oil, crambe abyssinica seed oil, lanolin, albolene, cetanol, isopropyl myristate, polyglycereol fat are taken
Acid esters and butylated hydroxyarisol mix, and heating water bath heats while stirring to 80 DEG C, until material is completely dissolved, obtain
Oily phase is kept the temperature spare；
(3) compound of glauber-salt and liquorice, 2-PHENYLBENZIMIDAZOLE-5-SULFONIC ACID sodium, glycerol and purified water is taken to mix, heating water bath is to 80 DEG C, Bian Jia
Hot side stirring obtains water phase until material is completely dissolved, and keeps the temperature spare；
(4) water phase is added in oily phase, 80 DEG C of insulated and stirred 5~10min, it is to be emulsified completely after, be cooled to 40 DEG C, then plus
Enter dissolved chlorophyll, propolis extract and ethyl-para-hydroxybenzoate, continue stirring to room temperature to get.
6. the preparation method of the antiphlogistic and analgesic ointment according to claim 5 containing chlorophyll, which is characterized in that the wood bamboo
The preparation method of seed oil includes the following steps: to take garcinia multiflora seed, clean, and dries, and garcinia multiflora seed benevolence is crushed into powder by decladding
Shape crosses 50 meshes, is subsequently placed in supercritical extraction reactor and carries out 1~2h of extraction, CO is arranged2Flow be 15~25L/h, extraction pressure
Power is 30~40MPa, and extraction temperature is 35~40 DEG C.
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| GB444276A (en) * | 1935-04-08 | 1936-03-18 | Paul Reinhold Grueter | An improved process for the manufacture of medicinal preparations containing chlorophyll or chlorophylline |
| CN1102090A (en) * | 1993-10-23 | 1995-05-03 | 中国科学院大连化学物理研究所 | Cosmetics prepared by using oenothera oil as raw material |
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