CN106306349A - Food-calling puffing fermented creep feed for piggies and preparation method thereof - Google Patents
Food-calling puffing fermented creep feed for piggies and preparation method thereof Download PDFInfo
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- CN106306349A CN106306349A CN201510378443.9A CN201510378443A CN106306349A CN 106306349 A CN106306349 A CN 106306349A CN 201510378443 A CN201510378443 A CN 201510378443A CN 106306349 A CN106306349 A CN 106306349A
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- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
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Abstract
The invention discloses a food-calling puffing fermented creep feed for piggies and a preparation method thereof, which belong to the technical fields of a feed, an enzyme preparation and microbial fermentation. The preparation method disclosed by the invention comprises the following steps of cleaning rice, corn, wheat and soybeans, soaking the materials, then mixing the raw materials with tomatoes, shelled eggs, whey powder, maltodextrin, brown sugar and glucose in proportion, grinding a mixture, boiling the mixture, boiling the mixture and disinfecting the mixture, then mixing the mixture with a composite bacterial strain liquid and a composite pre-blend feed in proportion for fermented enzymolysis, drying the material at low temperature, and puffing the material to prepare the food-calling puffing fermented creep feed. The food-calling puffing fermented creep feed is rich in yeast protein, small peptide, short-chain dextrin, glucose, oligosaccharide, lactic acid and probiotics, and has the characteristics of being excellent in palatability, balanced in amino acids, high in nutrition, easy to digest, low in content of anti-nutritional factors and the like.
Description
Technical field
The invention belongs to feedstuff, enzyme preparation and technical field of microbial fermentation, be specifically related to one
Plant piglet attractant expanded fermentation creep feed and preparation method thereof.
Background technology
China is populous nation, Ye Shi aquaculture big country.2013, the animal husbandry output value reached 2.5
Trillion yuan, meat, fowl egg, milk and output of aquatic products be respectively 84,820,000 tons, 28,320,000
Ton, 38,000,000 tons and 58,730,000 tons.Along with the development of aquaculture, feed industrial development speed
Spend surprising, nearly 1.93 hundred million tons of national commercial feed yield in 2013.Expect 12 ends,
Whole nation feedstuff production capacity is up to 3.5 hundred million tons, and yield is up to 2.5 hundred million tons.China's pig-breeding
Big country.Within 2013, China live pig delivers 7.19 hundred million for sale, sow livestock on hand more than 5,000 ten thousand, Carnis Sus domestica
Yield reaches 53,350,000 tons, needs nearly 2.0 hundred million tons of pig feed, wherein children poultry in age actual year
Feedstuff is up to 15,000,000 tons, and the feed for piglet is more than 6,000,000 tons.
It is known that: improve the palatability of the feed for piglet, feed intake and digestibility always
The target that feedstuff circle and cultivation industry are pursued, receives much concern in recent years.But, as feedstuff
Ingredient raw material inherently have some palatability problems, as the fishy smell material in Semen sojae atricolor,
Lectin in beans, the thioglycoside in rapeseed meal, the list in many corn
Rather, fat oxidative breakdown product etc., all the palatability of feedstuff can be impacted.
In order to improve palatability and the feed intake of the feed for piglet, for a long time people be all with
Flavouring agent and sweeting agent cover abnormal flavour therein and bad mouthfeel.Flavouring agent lures as one
Food agent, affects not quite substantially on the palatability of feedstuff and the feed intake of poultry.Sweeting agent is main
The palatability improving feedstuff, still based on saccharin sodium, is had certain effect, but has by composition
Metal bitterness, the most also has rear bitter taste, nonnutritive function, to a certain degree limits
Its effect and range are made.
At present, China's feed processing technology has been achieved for major progress.With regard to material shape
Speech, from peasant household's water from washing rice liquid feed to commercialization powder, develops into business from commercialization powder
Change pellet, sprayed particle feedstuff after developing deeply to commercialization liquid.It is known that it is newborn
One of feeding piglet key technology is to improve palatability and the feed intake of the feed for piglet.Research
Showing, suckling piglet more likes fermented feed, expanded pellet diet, cured feed and liquid feed.
Therefore, how to be improved by production technology and improve piglet feed intake and be one and be worth research
New problem.
Summary of the invention
It is contemplated that overcome, pig starter feed palatability is low, digestibility is low and the high difficulty of diarrhea rate
Topic, it is provided that one stops albumen, little peptide, short chain dextrin, oligosaccharide, Portugal rich in microbial bacteria
Grape sugar, lactic acid and probiotic bacteria, have that palatability is splendid, amino acid balance, nutrition high, easily
The novel attractant of the piglet expanded fermentation religion groove of the features such as digestion is low with antinutritional factor content
Material.
The technical solution used in the present invention is as follows:
The preparation method of a kind of piglet attractant expanded fermentation creep feed, comprises the steps:
1) by little for the 1-30 that is soaked in water at normal temperatures after rice, Semen Maydis, Semen Tritici aestivi, washing soybean
Time, prepare raw material soaking solidliquid mixture；
2) by raw material soaking solidliquid mixture, Fructus Lycopersici esculenti, egg, fish flour, whey powder, wheat
After the mixing of bud dextrin, brown sugar, glucose, grind pulping, be warmed up to 80-100 DEG C, under normal pressure
Steaming and decocting, ripening, sterilizing 20-60 minute, be cooled to 20-35 DEG C, prepares creep feed nutrition
Slurry；
3) after creep feed nutrient paste, composite bacteria liquid, compound premixed feed being mixed,
Solid fermentation enzymolysis 12-72 hour under the conditions of 15-35 DEG C, then low temperature is done under the conditions of 15-30 DEG C
Dry, be broken into attractant fermented feed；
4) by after expanded for attractant fermented feed, attractant expanded fermentation creep feed is prepared.
In one embodiment of the invention, by rice, Semen Maydis, Semen Tritici aestivi, Semen sojae atricolor and water with
After weight ratio is 100:100～500:100～200:100～400:400～800 mixing, often
Temperature is lower soaks 6-12 hour, prepares raw material soaking solidliquid mixture.
Wherein, the weight ratio of rice, Semen Maydis, Semen Tritici aestivi, Semen sojae atricolor and water is preferably:
100:150～350:100～150:150～300:500～650.
In another embodiment of the invention, by raw material soaking solidliquid mixture, western red
Fructus Kaki, egg, fish flour, whey powder, maltodextrin, brown sugar, glucose are by weight
After 100:10～30:5～20:1～5:1～5:0.5～2:1～4:0.1～2 mixing, grind pulping,
Atmospheric cooking under the conditions of being warmed up to 80-100 DEG C, ripening and sterilizing are cooled to after 20-60 minute
20-35 DEG C, prepare creep feed nutrient paste.
Wherein, raw material soaking solidliquid mixture, Fructus Lycopersici esculenti, egg, inlet steam fish flour,
Whey powder, maltodextrin, brown sugar, the weight ratio of glucose are preferably:
100:15～20:10～15:2～4:3～5:0.5～1:1～2:0.5～1.
In another embodiment of the invention, by creep feed nutrient paste, composite bacteria liquid,
After compound premixed feed is by weight mixing for 100:2～15:1～5,25-35 DEG C of condition
Lower solid fermentation enzymolysis is after 12-24 hour, then cold drying under the conditions of 15-30 DEG C, broken
Become attractant fermented feed.
Wherein, creep feed nutrient paste, composite bacteria liquid, the weight ratio of compound premixed feed
It is preferably: 100:5～12:2～3.
Wherein, described composite bacteria liquid be mainly composed of lactic acid bacteria, bacillus subtilis and
Yeast.
Preferably, described lactic acid bacteria is lactobacillus casei CGMCC No.8149, and described is withered
Grass bacillus cereus is bacillus subtilis CGMCC No.8148.
Wherein, described bacillus subtilis, lactobacillus casei have good stomach juice-resistant,
The anti-adversities such as resistance to intestinal juice, bile tolerance (bacillus cereus is high temperature resistant) and product acid, product enzyme, suppression
The prebiotic function such as pathogen, separates from healthy animal intestinal for applicant, selection-breeding obtains,
And it is the most micro-within 11st, to be preserved in China Committee for Culture Collection of Microorganisms in JIUYUE in 2013
Bio-Centers, is called for short CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Institute of Microorganism, Academia Sinica, deposit number be respectively as follows: CGMCC No.8148,
CGMCC No.8149。
The bacillus subtilis CGMCC No.8148 of selection-breeding of the present invention is at artificial simulation gastric juices
Processing 3h in (pH2.0～4.0), survival rate is more than 65.8%；In artificial simulation intestinal juice
Processing 3h, survival rate is 74.7%；24h is processed at Fel Sus domestica saline solution (concentration 0.3～3g/kg),
Survival rate is more than 51.2%；85～100 DEG C process 3min, and survival rate is more than 11.2%；Right
Streptomycin, polymyxin B, 3 kinds of antibiosis of lincomycin have drug resistance；Can produce certain
The protease of amount, lipase, amylase, cellulase, xylanase.
The lactobacillus casei CGMCC No.8149 of institute of the present invention selection-breeding is at artificial simulation gastric juices
Processing 3h in (pH2.0～4.0), survival rate is more than 43.1%；In artificial simulation intestinal juice
Processing 3h, survival rate is 63.5%；24h is processed at Fel Sus domestica saline solution (concentration 0.3～3g/kg),
Survival rate is more than 8.6%；To cefepime, gentamycin, spectinomycin, streptomycin,
Polymyxin B, ciprofloxacin, 7 kinds of antibiosis of vancomycin have drug resistance；To escherichia coli
K88, K99 all have good inhibiting effect, antibacterial circle diameter be respectively 16.13mm,
14.09mm。
Wherein, described compound premixed feed, by commercially available piglet compound microelement, son
Pig compound vitamin, lysine hydrochloride, DL-methionine, L-threonine, phosphoric acid hydrogen
Calcium, calcium formate, sodium chloride, compound enzymic preparation, antioxidant are by weight
100:5～20:10～30:1～5:5～10:5～20:40～80:5～25:10～50:1～5 is mixed to prepare, excellent
Select 100:10～15:15～20:2～3:6～8:10～15:50～65:10～15:20～30:2～3.
The present invention also provides for a kind of piglet attractant expanded fermentation creep feed, and it is by institute of the present invention
The preparation method of the piglet attractant expanded fermentation creep feed stated prepares.
The present invention by after rice, Semen Maydis, Semen Tritici aestivi, washing soybean, immersion, with Fructus Lycopersici esculenti,
Egg, inlet steam fish flour, whey powder, maltodextrin, brown sugar, glucose mix in proportion
After conjunction, grinding, steaming and decocting, ripening and sterilizing, then with composite bacteria liquid, compound close feedstuff in advance
After being proportionally mixed for fermentation enzymolysis, cold drying, expanded after, prepare the expanded fermentation of attractant
Creep feed.
The novel attractant of the piglet expanded fermentation creep feed that the present invention prepares is stopped rich in microbial bacteria
Albumen, little peptide, short chain dextrin, glucose, oligosaccharide, lactic acid and probiotic bacteria, have suitable
The spies such as mouthful property is splendid, amino acid balance, nutrition are high, easy to digest and antinutritional factor content is low
Point.The attractant expanded fermentation creep feed of the present invention is substantially better than matched group feedstuff, reaches completely
Having arrived good palatability, feed intake is high, effect easy to digest, somatotrophic.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.
The resistance of embodiment 1 strain and biology performance measure
2 strain strains: bacillus subtilis CGMCC No.8148 and lactobacillus casei
The biology performance of CGMCC No.8149 and anti-adversity measure:
1) preparation of bacillus subtilis CGMCC No.8148 culture: by Storage in refrigerator
Slant strains be inoculated in LB seed culture medium activation, 37 DEG C, 100rpm cultivate 16h,
Obtain；
2) preparation of lactobacillus casei CGMCC No.8149 culture: by Storage in refrigerator
Slant strains is inoculated in MRS seed culture medium activation, 30 DEG C, 150rpm cultivate 16h,
Obtain；
3) acid resistance measures: be inoculated in respectively by 10% inoculum concentration by the culture of above-mentioned preparation
In the artificial simulation gastric juices of pH value 2.0,3.0,4.0, again to adjust pH value be 2.0,3.0,
4.0,0h countings compare, and 3h sampling, is entered by 10 times of serial dilutions with 0.85% normal saline
Row viable plate count, calculates survival rate.
2 strain strains of selection-breeding of the present invention: bacillus subtilis CGMCC No.8148 and cheese
Lactobacillus CGMCC No.8149 survival results in gastric acid is shown in Table 1.
The preparation of artificial simulation gastric juices: measure 9.5%～10.5% concentrated hydrochloric acid 16.4 milliliters, add steaming
Distilled water, to 1000 milliliters, does basis simulated gastric fluid, adjusts pH value with hydrochloric acid or sodium hydroxide
2.0,3.0,4.0, steam sterilization 15 minutes at 121 DEG C, add pepsin by 1% addition
Enzyme, by 0.22 μm sterilised membrane filter, makes simulated gastric fluid, takes 5mL (4.5mL) subpackage
In test tube.
Table 12 strain strain survival rate in artificial simulation gastric juices
4) resistance to intestinal juice measures: by 5% inoculum concentration, the culture of above-mentioned preparation is inoculated in people respectively
In work simulated intestinal fluid, 0h counting compares, and 3h sampling presses 10 times with the normal saline of 0.85%
Serial dilution, carries out viable plate count, calculates survival rate.
2 strain strains of selection-breeding of the present invention: bacillus subtilis CGMCC No.8148, cheese
Lactobacillus CGMCC No.8149 survival results in intestinal juice is shown in Table 2.
The preparation of artificial simulation intestinal juice: weigh 6.8g, adds distilled water to 1000 milliliters, does base
Plinth simulated intestinal fluid, steam sterilization 15 minutes at 121 DEG C, add Trypsin by 1% addition
Enzyme, by 0.22 μm sterilised membrane filter, makes simulated gastric fluid, takes 5mL (4.5mL) subpackage
In test tube.
Table 22 strain strain survival rate in artificial simulation intestinal juice
| Survival rate/% | |
| The process time | 3h |
| Bacillus subtilis CGMCC No.8148 | 74.7 |
| Lactobacillus casei CGMCC No.8149 | 63.5 |
5) bile tolerance measures: by the culture of 2 strain strains of above-mentioned preparation, by 10% inoculation
Amount is inoculated in the LB solution of 0.03%, 0.1%, 0.2%, 0.3% variable concentrations Fel Sus domestica salt respectively
In, 0h counting compares, and 24h samples with 0.85% normal saline based on 10 times of serial dilutions
Number, carries out viable plate count, calculates survival rate.
2 strain strains of institute of the present invention selection-breeding: bacillus subtilis CGMCC No.8148, dry
Lactobacillus paracasei CGMCC No.8149 survival results in cholate is shown in Table 3.
The preparation of cholate: in the LB fluid medium prepared add 0.03%, 0.1%,
0.2%, the Fel Sus domestica salt of 0.3% variable concentrations, steam sterilization 30 minutes at 121 DEG C, aseptic
Under the conditions of, take the Fel Sus domestica salt of 5mL 0.03%, 0.1%, 0.2%, 0.3% variable concentrations respectively
Solution is sub-packed in test tube.
Table 32 strain strain processes the survival rate of 24h in variable concentrations Fel Sus domestica salt
6) the high temperature resistant mensuration of bacillus cereus: by the training of the Bacillus subtilis strain of above-mentioned preparation
Support thing 3500rpm and be centrifuged 30min, go culture supernatant, with 0.85% normal saline by 10
Times serial dilution to certain multiple, respectively at 85 DEG C, 90 DEG C, 95 DEG C, at 100 DEG C of water-baths
Reason 3min, flowing water cools down, and without the strain culturing of water-bath as comparison under room temperature, uses
Colony counting method calculates the survival rate after water-bath.
Table 4 bacillus subtilis is at the survival rate/% of different high-temperature process 3min
7) drug: use quick paper disk method.Take the culture of appropriate tested antibacterial,
Being spread evenly across Nutrient agar surface, after bacterium solution absorbs, stick drug sensitive test paper, 37 cultivate
18h, observed result, and use vernier caliper measurement antibacterial circle diameter, judge according to diameter
The antibacterial sensitivity to medicine.
The drug result of table 52 strain strain
8) the product enzymatic determination of bacillus cereus:
Use flat board transparent circle method.Respectively cellulase culture medium, protease culture medium,
The spore that amylase culture medium, xylanase culture medium separate with dibbling in lipase culture medium
Bacillus, every kind of process sets 3 repetitions, cultivates 36h for 37 DEG C, and that observes flat board transparent circle has that it's too late
Size, if forming transparent bacterium colony or the circle of confusion in periphery of bacterial colonies, then proves bacillus cereus
Corresponding enzyme can be produced, measure transparent circle diameter (H) with slide gauge respectively and bacterium colony is straight
Footpath (C), calculates both ratios (H/C).
The product enzymatic determination result of table 6 Bacillus subtilis strain
9) acidity test is produced:
Use bromocresol purple plate that lactobacillus casei CGMCC No.8149 is carried out organic acid product
It is fixed to measure.It is then yellow by purple stain at periphery of bacterial colonies flat board that thalline produces acid, surveys respectively with slide gauge
Amount yellow loop diameter (H) and colony diameter (C), calculate both ratios (H/C).
The product acidity test result of table 7 species L. casei
10) bacteriostatic test: use agar punching diffusion method that common pathogen is carried out antibacterial survey
Fixed.
A, in the test tube equipped with 10mL nutrient culture medium, activate two strain pathogenic bacterium:
K88, K99,37 DEG C of constant temperature culture 20h；
B, the flat board of cut-off footpath 90mm, injecting 100uL concentration is 1*108CFU/ml indicates
Bacterium bacterium solution, is then injected into the LB solid medium of warm, after mixing, after agar cools down,
On agar, the hole of four a diameter of 3mm is made a call to card punch；
C, add sample: in each hole, add the fermented liquid supernatant of 20uL lactobacillus casei
Liquid, the repetition of 2, each sample.The flat board adding sample is placed in 37 DEG C of calorstats, cultivates
After 16～18h, take out and measure inhibition zone size.
The table 8 lactobacillus casei antibacterial result to common pathogen
The preparation of embodiment 2 lactobacillus casei fermentation liquid
1) rejuvenation cultivated by flat board: species L. casei is inoculated in MRS plating medium
On, cultivate 16h in 37 DEG C, make lactobacillus casei bacterium rejuvenation, and form single bacterium colony, picking
Single bacterium colony, on inoculation medium, cultivates 14h for 37 DEG C；
2) preparation of first order seed: by step 1) cultivate species L. casei switching dress
Having in 300ml MRS liquid culture medium 2L shaking flask, 37 DEG C of quiescent culture 18h, to logarithm
In the later stage, obtain first order seed；
3) preparation of secondary seed: by step 2) first order seed prepared is transferred to equipped with 75L
In the 100L seed tank of MRS seed culture medium, temperature 37 DEG C, rotating speed 80rpm, cultivates
18h, obtains secondary seed solution.
4) preparation of lactobacillus casei fermentation liquid: by step 3) secondary seed solution prepared by
Inoculum concentration according to 1.5% is inoculated into 0.75m3In the fermentation tank of fermentation medium, temperature
37 DEG C, rotating speed 80rpm, tank pressure 0.05Mpa, ventilating ratio: 1:0.2, cultivate 18h,
It is 4.6 × 10 to viable count9Cfu/ml lactobacillus casei fermentation liquid.
Described fermentation medium is: glucose 2%, soy peptone 1.8%, yeast extract
1.5%, ammonium sulfate 0.75%, dipotassium hydrogen phosphate 0.4%, sodium chloride 0.6%, magnesium sulfate
0.04%；
The preparation of embodiment 3 fermentation of bacillus subtilis liquid
1) rejuvenation cultivated by flat board: Bacillus subtilis strain is inoculated in BPY plating medium
On, cultivate 18h in 37 DEG C, make bacillus subtilis rejuvenation, and form single bacterium colony, picking
Single bacterium colony, on inoculation medium, cultivates 30h for 37 DEG C；
2) preparation of first order seed: by step 1) cultivate Bacillus subtilis strain switching
On Fructus Solani melongenae bottle BPY slant medium, cultivate 14h, make to be in late log phase, obtain one for 36 DEG C
Level seed；
3) preparation of secondary seed: by step 2) the first order seed sterilized water prepared makes
Bacteria suspension, is inoculated in the 100L seed tank equipped with 60L BPY seed culture medium, temperature
37 DEG C, rotating speed 300rpm, tank pressure 0.05MPa, ventilating ratio: 1: 0.8, cultivate 12h,
Obtain secondary seed solution.
4) preparation of fermentation of bacillus subtilis liquid: by step 3) secondary seed solution prepared
It is inoculated into 6m according to the inoculum concentration of 1%3In the fermentation tank of fermentation medium, temperature
30-40 DEG C, rotating speed 280rpm, tank pressure 0.05Mpa, ventilating ratio: 1: 0.8, cultivate
24h, obtains spore production rate more than 90%, and viable count is 5.48 × 109The hay of cfu/ml
Fermentation of bacillus liquid；
Described fermentation medium is: glucose 1.5%, soy peptone 1.5%, bean cake
1.5%, ammonium sulfate 0.75%, sodium chloride 0.6%, magnesium sulfate 0.05%；PH is natural.
The preparation of embodiment 4 saccharomyces cerevisiae CGMCC No.6560 fermentation liquid
Preparation saccharomyces cerevisiae CGMCC No.6560 (is disclosed in patent CN
201210430095.1) seed culture medium: 1.0% yeast leaching powder, 2.0% peptone, 2.0% Portugal
Grape sugar, 100ml distilled water.121 DEG C, 15min sterilizing.From cultured saccharomyces cerevisiae K1
On inclined-plane, picking one ring is linked in seed culture medium.Condition of culture: 28 DEG C, 180rpm,
36h。
Fermentation by saccharomyces cerevisiae culture medium: 1.5% casein peptone, 2.5% yeast leaching powder, 3.0% Portugal
Grape sugar, 0.24% potassium dihydrogen phosphate, 1.6% dipotassium hydrogen phosphate, add distilled water.121 DEG C,
15min sterilizing.By 5% inoculum concentration access seed culture medium, condition of culture: 28 DEG C,
150rpm, 72h, prepared viable count is 3.63 × 109The ferment brewer yeast fermentation liquid of cfu/ml.
The preparation of embodiment 5 composite bacteria liquid
The lactobacillus casei fermentation liquid of embodiment 2 preparation, embodiment 3 prepare bacillus subtilis
The ferment brewer yeast fermentation liquid of fermentation liquid and embodiment 4 preparation is mixed in the ratio of 2:1:1
Close, prepare composite bacteria fermentation liquid.
The preparation of embodiment 6 attractant expanded fermentation creep feed
Weigh commercially available rice 10kg, Semen Maydis 30kg, Semen Tritici aestivi 15kg, Semen sojae atricolor 25kg and water
After 65kg mixing, soak 8 hours under the conditions of 30 DEG C, prepare 140kg (loss 5kg) raw material
Soak solidliquid mixture.
Raw material soaking solidliquid mixture 140kg, new fresh tomato 25kg, egg (are gone
Shell) 15kg, inlet steam fish flour 3.5kg, whey powder 5.5kg, maltodextrin 1.5kg, brown sugar
After 1.5kg, glucose 1kg mixing, via ball mill grinding pulping, it is warmed up to 95-100 DEG C
Under the conditions of atmospheric cooking, ripening and sterilizing be cooled to 28-30 DEG C after 40 minutes, prepare 190kg
Creep feed nutrient paste (moisture and other loss 3kg).
By creep feed nutrient paste 190kg, composite bacteria liquid 20kg, compound premix feedstuff
5.25kg (wherein containing compound microelement 2.0kg, compound vitamin 0.3kg, 98.5% rely ammonia
Acid hydrochloride 0.4kg, DL-methionine 0.05kg, L-threonine 0.15kg, calcium hydrogen phosphate
0.3kg, calcium formate 1.2kg, sodium chloride 0.3kg, compound enzymic preparation 0.5kg, antioxidant
0.05kg) after mixing, under the conditions of 30-32 DEG C, solid fermentation enzymolysis is after 12 hours, then
Cold drying under the conditions of 25-35 DEG C, be broken into 103kg attractant fermentation creep feed (fermentation loss
112.25kg is added up) with loss of water.
By 103kg attractant fermented feed after conventional feed bulking machine is expanded, prepare
102.5kg attractant expanded fermentation creep feed (expanded loss adds up 0.5kg).
The preparation of embodiment 7 attractant expanded fermentation creep feed
Weigh commercially available rice 15kg, Semen Maydis 25kg, Semen Tritici aestivi 17kg, Semen sojae atricolor 23kg and clean
After water 60kg mixing, soak 10 hours under the conditions of 35 DEG C, prepare 135kg raw material soaking
Liquid (loss 5kg).
By raw material soaking liquid 135kg, new fresh tomato 21kg, egg (shelling) 19kg, import
Steam fish flour 4kg, whey powder 5kg, maltodextrin 1kg, sucrose (brown sugar) 2kg, Fructus Vitis viniferae
After sugar 1kg mixing, via ball mill grinding pulping, under the conditions of being warmed up to 90-95 DEG C, normal pressure steams
Boil, ripening and sterilizing are cooled to 28-30 DEG C after 60 minutes, prepare 185kg creep feed nutrient paste
(moisture and other loss 3kg).
By creep feed nutrient paste 185kg, composite bacteria liquid 10kg, compound premix feedstuff
5.05kg (wherein phosphoric acid hydrogen calcium 0.25kg, calcium formate 1kg, sodium chloride 0.25kg,
98.5% lysine hydrochloride 0.4kg, DL-methionine 0.04kg, L-threonine 0.16kg, multiple
Close trace element 2kg, compound enzymic preparation 0.6kg, compound vitamin 0.3kg, antioxidant
0.05kg) after mixing, under the conditions of 30-32 DEG C, solid fermentation enzymolysis is after 12 hours, then
Cold drying under the conditions of 25-35 DEG C, be broken into 102kg attractant fermentation creep feed (fermentation loss
98.05kg is added up) with loss of water.
By 102kg attractant fermented feed after conventional feed bulking machine is expanded, prepare
101.6kg attractant expanded fermentation creep feed (expanded loss adds up 0.4kg).
Ablactational baby pig feeding effect is tested by test example 1 attractant expanded fermentation creep feed
1 material and method
1.1 EXPERIMENTAL DESIGN and test feed
Taking controlled trial to design, each group sets three repetitions, each repetition 10 wean son
Pig, 10 days experimental periods.Test group ablactational baby pig feeds the food calling of the embodiment of the present invention 6 preparation
The expanded fermentation creep feed of property, the feedstuff that matched group ablactational baby pig feeds through 85-90 DEG C of granulation,
Raw material composition and ratio used by the feedstuff of control group fed are the most consistent with test group, difference
Essentially consist in without fermentation enzymolysis processing, matched group formula composition and trophic level such as following table
Shown in.
Table 1 matched group formula composition and trophic level
1.2 animals select and feeding and management
Selecting the piglet 60 of wean about 25 ages in days, the same day was driven sow away in wean, and piglet stays
In original place, then according to ablactational baby pig body weight random packet, often organize 3 hurdle pigs, 10, every hurdle
Piglet, puts into a hopper in every hurdle, be respectively put into comparison according to EXPERIMENTAL DESIGN and test is raised
Material, the concrete condition such as table 2 of test piglet.During test, column home cleaning is kept to be dried and protect
Temperature, piglet freely drinks water and searches for food, feed intake and diarrhoea situation, off-test during record
Early morning on the same day weighs on an empty stomach.
Table 2 matched group and test group piglet initial situation
1.3 measure project
Ablactational baby pig original body mass, end of term body weight, period feed consumption total amount, calculating diarrhea rate,
Average daily gain and feedstuff-meat ratio.
Diarrhea rate=diarrhoea head number/overall test piglet head number * 100%
Average daily gain=(end of term body weight-original body mass)/feed natural law
Feedstuff-meat ratio=consumption forage volume/(end of term body weight-original body mass)
1.4 statistical analysiss utilize SPSS17.0 software that test data is carried out statistical analysis.
2. result of the test
The biological activity companion's pellet of table 3 present invention feeding effect to ablactational baby pig
3, conclusion (of pressure testing)
As shown in table 3, ablactational baby pig is raised by biological activity companion's pellet of the present invention
Feeding effect ideal, its good palatability, feed intake is high, and daily gain is fast.With matched group phase
Ratio, the average daily ingestion amount of test group ablactational baby pig reaches 277.3 grams, improves than matched group
14.2%, significant difference (p < 0.05)；Average daily gain is 269.2 grams/day, with matched group
Compare, improve 24%, significant difference (p < 0.05)；Feedstuff-meat ratio reduces than matched group
0.09, significant difference (p < 0.05)；Comparing matched group, diarrhoea is obviously improved, and says
The attractant expanded fermentation creep feed of the bright present invention is substantially better than matched group feedstuff, is fully achieved
Good palatability, feed intake is high, effect easy to digest, somatotrophic.
Above content is only citing made for the present invention and explanation, affiliated the art
Technical staff described specific embodiment is made various amendment supplement or use
Similar mode substitutes, without departing from the design of invention or surmount the claims institute
The scope of definition, all should belong to protection scope of the present invention.
The above is only the preferred embodiment of the present invention, it is noted that lead for this technology
For the those of ordinary skill in territory, on the premise of without departing from the technology of the present invention principle, it is also possible to
Making some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (10)
1. the preparation method of a piglet attractant expanded fermentation creep feed, it is characterised in that
Comprise the steps:
1) by little for the 1-30 that is soaked in water at normal temperatures after rice, Semen Maydis, Semen Tritici aestivi, washing soybean
Time, prepare raw material soaking solidliquid mixture；
2) by raw material soaking solidliquid mixture, Fructus Lycopersici esculenti, egg, fish flour, whey powder, wheat
After the mixing of bud dextrin, brown sugar, glucose, grind pulping, be warmed up to 80-100 DEG C, under normal pressure
Steaming and decocting, ripening, sterilizing 20-60 minute, be cooled to 20-35 DEG C, prepares creep feed nutrition
Slurry；
3) after creep feed nutrient paste, composite bacteria liquid, compound premixed feed being mixed,
Solid fermentation enzymolysis 12-72 hour under the conditions of 15-35 DEG C, then low temperature is done under the conditions of 15-30 DEG C
Dry, be broken into attractant fermented feed；
4) by after expanded for attractant fermented feed, attractant expanded fermentation creep feed is prepared.
2. the preparation side of piglet attractant expanded fermentation creep feed as claimed in claim 1
Method, it is characterised in that with weight ratio be by rice, Semen Maydis, Semen Tritici aestivi, Semen sojae atricolor and water
After 100:100～500:100～200:100～400:400～800 mixing, soak at normal temperatures
6-12 hour, prepare raw material soaking solidliquid mixture.
3. the preparation side of piglet attractant expanded fermentation creep feed as claimed in claim 2
Method, it is characterised in that the weight ratio of rice, Semen Maydis, Semen Tritici aestivi, Semen sojae atricolor and water is:
100:150～350:100～150:150～300:500～650.
4. the preparation side of piglet attractant expanded fermentation creep feed as claimed in claim 1
Method, it is characterised in that by raw material soaking solidliquid mixture, Fructus Lycopersici esculenti, egg, fish flour,
Whey powder, maltodextrin, brown sugar, glucose by weight for 100:10～30:5～20:1～
After 5:1～5:0.5～2:1～4:0.1～2 mixing, grind pulping, be warmed up to 80-100 DEG C, often
Pressure steaming and decocting, ripening and sterilizing 20-60 minute, be cooled to 20-35 DEG C, prepares creep feed nutrition
Slurry.
5. the preparation side of piglet attractant expanded fermentation creep feed as claimed in claim 4
Method, it is characterised in that raw material soaking solidliquid mixture, Fructus Lycopersici esculenti, egg, inlet steam
Fish flour, whey powder, maltodextrin, brown sugar, the weight ratio of glucose be:
100:15～20:10～15:2～4:3～5:0.5～1:1～2:0.5～1.
6. the preparation side of piglet attractant expanded fermentation creep feed as claimed in claim 1
Method, it is characterised in that by creep feed nutrient paste, composite bacteria liquid, compound premixed feed
After for 100:2～15:1～5 mixing, solid fermentation enzymolysis under the conditions of 25-35 DEG C
After 12-24 hour, then cold drying under the conditions of 15-30 DEG C, be broken into attractant fermentation raise
Material.
7. the preparation side of piglet attractant expanded fermentation creep feed as claimed in claim 6
Method, it is characterised in that creep feed nutrient paste, composite bacteria liquid, compound premixed feed
Weight ratio is: 100:5～12:2～3.
8. piglet attractant expanded fermentation creep feed as described in any one of claim 1～7
Preparation method, it is characterised in that described composite bacteria liquid is mainly composed of lactic acid bacteria, withered
Grass bacillus cereus and yeast, described lactic acid bacteria is lactobacillus casei CGMCC
No.8149, described bacillus subtilis is bacillus subtilis CGMCC No.8148.
9. piglet attractant expanded fermentation creep feed as described in any one of claim 1～7
Preparation method, it is characterised in that it is characterized in that, described compound premixed feed is by multiple
Close trace element, compound vitamin, lysine hydrochloride, DL-methionine, L-threonine,
Calcium hydrogen phosphate, calcium formate, sodium chloride, compound enzymic preparation, antioxidant are by weight
100:5～20:10～30:1～5:5～10:5～20:40～80:5～25:10～50:1～5, preferably
100:10～15:15～20:2～3:6～8:10～15:50～65:10～15:20～30:2～3 is mixed to prepare.
10. according to the piglet attractant expanded fermentation creep feed described in any one of claim 1-9
The piglet attractant expanded fermentation creep feed prepared of preparation method.
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Cited By (6)
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| CN106819481A (en) * | 2017-02-15 | 2017-06-13 | 山东碧蓝生物科技有限公司 | A kind of full price fermented feed for piglet religion groove and preparation method thereof |
| CN107048040A (en) * | 2017-06-05 | 2017-08-18 | 广东大广生物科技有限公司 | A kind of expanded fermentation raw material of weanling pig and preparation method thereof |
| CN107156428A (en) * | 2017-05-16 | 2017-09-15 | 溧阳正昌饲料科技有限公司 | A kind of sucking pig curing activated feed and preparation method thereof |
| CN107373157A (en) * | 2017-09-25 | 2017-11-24 | 安顺市平坝区鸿黔养殖场 | The production technology of the cold granulation fermented feed of one boar growth promotion |
| CN109393155A (en) * | 2018-11-20 | 2019-03-01 | 合肥五粮泰生物科技有限公司 | A kind of preparation method of injection curing beans egg feed |
| CN111264686A (en) * | 2020-03-19 | 2020-06-12 | 湖南粒丰生物科技有限公司 | Preparation method of antibiotic-free fermented soft-particle creep feed for suckling piglets |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106819481A (en) * | 2017-02-15 | 2017-06-13 | 山东碧蓝生物科技有限公司 | A kind of full price fermented feed for piglet religion groove and preparation method thereof |
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| CN107156428A (en) * | 2017-05-16 | 2017-09-15 | 溧阳正昌饲料科技有限公司 | A kind of sucking pig curing activated feed and preparation method thereof |
| CN107048040A (en) * | 2017-06-05 | 2017-08-18 | 广东大广生物科技有限公司 | A kind of expanded fermentation raw material of weanling pig and preparation method thereof |
| CN107373157A (en) * | 2017-09-25 | 2017-11-24 | 安顺市平坝区鸿黔养殖场 | The production technology of the cold granulation fermented feed of one boar growth promotion |
| CN109393155A (en) * | 2018-11-20 | 2019-03-01 | 合肥五粮泰生物科技有限公司 | A kind of preparation method of injection curing beans egg feed |
| CN111264686A (en) * | 2020-03-19 | 2020-06-12 | 湖南粒丰生物科技有限公司 | Preparation method of antibiotic-free fermented soft-particle creep feed for suckling piglets |
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