CN107373157A - The production technology of the cold granulation fermented feed of one boar growth promotion - Google Patents

The production technology of the cold granulation fermented feed of one boar growth promotion Download PDF

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Publication number
CN107373157A
CN107373157A CN201710872788.9A CN201710872788A CN107373157A CN 107373157 A CN107373157 A CN 107373157A CN 201710872788 A CN201710872788 A CN 201710872788A CN 107373157 A CN107373157 A CN 107373157A
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China
Prior art keywords
fermentation
fermented feed
production technology
growth promotion
zymotic fluid
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CN201710872788.9A
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Chinese (zh)
Inventor
严国萍
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Anshun City Guizhou Pingba District Hung Farms
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Anshun City Guizhou Pingba District Hung Farms
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Priority to CN201710872788.9A priority Critical patent/CN107373157A/en
Publication of CN107373157A publication Critical patent/CN107373157A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/30Feeding-stuffs specially adapted for particular animals for swines
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14Pretreatment of feeding-stuffs with enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P39/00Processes involving microorganisms of different genera in the same process, simultaneously
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/065Azotobacter
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225Lactobacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/32Mycobacterium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/42Salmonella

Abstract

The invention discloses the production technology of the cold granulation fermented feed of a boar growth promotion, comprise the following steps:The raw material for needing to ferment are crushed first,The softening of immersion immersion afterwards,Choose medium component,And enough honey and polypeptide are added into culture medium,And it is dissolved in glucose solution,Emulsification treatment is carried out after stirring,By incubated formation zymotic fluid,After biomass ferment enzyme being added after fermentation substrate and fermentation broth agitation are well mixed,The fermentation of closed constant temperature is carried out again,Filtered,Filter out and carry out drying after zymotic fluid therein and be dried to obtain fermented feed,Individually configuration and activation,The change of otherness can be carried out according to different demands,Fermentation substrate is enabled to obtain the processing of more preferable otherness,It can promote to decompose,Advantageously form albumen for being easy to animal to absorb etc.,And entirely technique is simple,It is workable,It is and not high for equipment requirement,Use can be widely popularized.

Description

The production technology of the cold granulation fermented feed of one boar growth promotion
Technical field
The present invention relates to breeding feed technical field, the production work of the cold granulation fermented feed of a specially boar growth promotion Skill.
Background technology
With the continuous improvement of people's living standards, requirement of the people for food sanitation safe is also higher and higher, Therefore, for as the edible extensive pork the most normal of people, people have also progressively paid attention to its breeding process.And with The popularization of fermented feed concept, its application are also constantly being generalized.
Fermented feed refer to it is artificial it is controllable under the conditions of, with vegetalitas agricultural byproducts or the disused dreg of food processing factory For primary raw material, by the metabolism of microorganism, the ANFs in raw material is decomposed, synthesis, generation is more easy to by livestock and poultry Feeding, absorb nutrient and the biological feedstuff or feedstuff of nonhazardous effect.Various enzymes and decomposition in fermented feed Efficiency of feed utilization can be effectively improved;Secondly, fermented feed can with feed change albumen form and amino acid ratio, by nothing Machine nitrogen, vegetable protein are converted into high-quality mycoprotein;In addition, during feed fermentation, a large amount of microbial cells breedings, production Raw abundant vitamin and UGF, after livestock and poultry eat fermented feed, can form strong beneficial micro- life in enteron aisle Species group advantage, so as to suppress pathogenic flora, serve the effect that general antibiotic is unable to reach, avoid abuse of antibiotics to The harm that food security is brought.
But fermentation operation is complicated in existing preparation technology, and nutritional ingredient is than relatively low, also very big one Split-off is supported and is not released, and in the operation of reality, zymotechnique is more complicated, it is difficult to large batch of to realize industry Metaplasia is produced.
The content of the invention
To achieve the above object, the present invention provides following technical scheme:The life of the cold granulation fermented feed of one boar growth promotion Production. art, comprise the following steps:
(1)The pretreatment of fermentation substrate, it would be desirable to which the raw material of fermentation are crushed first, the softening of immersion immersion afterwards 2-4 days;
(2)The configuration and activation of zymotic fluid, medium component is chosen, and enough honey and polypeptide are added into culture medium, and And be dissolved in glucose solution, emulsification treatment is carried out after stirring, zymocyte liquid is added thereto after emulsification, By incubated formation zymotic fluid;
(3)Fermenting raw materials, by step(1)In fermentation substrate and step(2)In fermentation broth agitation it is well mixed after add life After fermentation of materials enzyme, clear water is added thereto and causes water content in 30-40%, then carry out the fermentation of closed 30-40 DEG C of constant temperature, send out The ferment time is 5-10 days;
(4)Dehydrate, step will be passed through(3)Product after fermentation is filtered, and is dried after filtering out zymotic fluid therein It is dry to be dried to obtain fermented feed.
As a preferred technical solution of the present invention, in step(2)In, zymocyte liquid is by nitrogen-fixing bacteria, Bacillus acidi lactici, bright Beading bacterium, mycobacteria, red spirillum and salmonella composition.
As a preferred technical solution of the present invention, in step(3)In, the biomass ferment enzyme is by lactic dehydrogenase In enzyme, cellulase, hemicellulose enzyme, glutathione peroxidase, mannonase zytase, b- dextranases extremely Few three kinds are formed with waiting mass ratio to be well mixed
As a preferred technical solution of the present invention, in step(3)In, detection specific method of the water content in 30-40%:With Hand pinch it is agglomerating do not drip, one touch i.e. dissipate for standard.
Compared with prior art, the beneficial effects of the invention are as follows:Pre-processed by the way that fermentation substrate is passed through, it is possible to increase base The softening degree of matter, preferably strain processing fermentation it can decompose, it is possible to increase the conversion ratio of material, and zymotic fluid is carried out Individually configuration and activation, the change of otherness can be carried out according to different demands, enables to fermentation substrate to obtain more preferably The processing of otherness, and can promote to decompose by using various enzymes during fermentation, advantageously form and be easy to animal to inhale Albumen of receipts etc., and whole technique is simple, workable and not high for equipment requirement, can be made by being widely popularized With.
Brief description of the drawings
Fig. 1 is preparation method schematic flow sheet of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made Embodiment, belong to the scope of protection of the invention.
Embodiment 1
The production technology of the cold granulation fermented feed of one boar growth promotion, comprises the following steps:
(1)The pretreatment of fermentation substrate, it would be desirable to which the raw material of fermentation are crushed first, the softening of immersion immersion afterwards 2 days;
(2)The configuration and activation of zymotic fluid, medium component is chosen, and enough honey and polypeptide are added into culture medium, and And be dissolved in glucose solution, emulsification treatment is carried out after stirring, zymocyte liquid is added thereto after emulsification, Zymocyte liquid is made up of nitrogen-fixing bacteria, Bacillus acidi lactici, leukonid, mycobacteria, red spirillum and salmonella, and process is incubated Form zymotic fluid;
(3)Fermenting raw materials, by step(1)In fermentation substrate and step(2)In fermentation broth agitation it is well mixed after add life After fermentation of materials enzyme, the biomass ferment enzyme is by lactic dehydrogenase, cellulase, hemicellulose enzyme, glutathione peroxidase At least three kinds in enzyme, mannonase zytase, b- dextranases with etc. mass ratio is well mixed forms, add thereto Clear water is added to cause water content 30%, detection specific method of the water content 30%:With hand pinch it is agglomerating do not drip, one touch i.e. dissipate for mark Standard, then the fermentation of closed 30 DEG C of constant temperature is carried out, fermentation time is 5 days;
(4)Dehydrate, step will be passed through(3)Product after fermentation is filtered, and is dried after filtering out zymotic fluid therein It is dry to be dried to obtain fermented feed.
Embodiment 2
The production technology of the cold granulation fermented feed of one boar growth promotion, comprises the following steps:
(1)The pretreatment of fermentation substrate, it would be desirable to which the raw material of fermentation are crushed first, the softening of immersion immersion afterwards 3 days;
(2)The configuration and activation of zymotic fluid, medium component is chosen, and enough honey and polypeptide are added into culture medium, and And be dissolved in glucose solution, emulsification treatment is carried out after stirring, zymocyte liquid is added thereto after emulsification, Zymocyte liquid is made up of nitrogen-fixing bacteria, Bacillus acidi lactici, leukonid, mycobacteria, red spirillum and salmonella, and process is incubated Form zymotic fluid;
(3)Fermenting raw materials, by step(1)In fermentation substrate and step(2)In fermentation broth agitation it is well mixed after add life After fermentation of materials enzyme, the biomass ferment enzyme is by lactic dehydrogenase, cellulase, hemicellulose enzyme, glutathione peroxidase At least three kinds in enzyme, mannonase zytase, b- dextranases with etc. mass ratio is well mixed forms, add thereto Clear water is added to cause water content 35%, detection specific method of the water content 35%:With hand pinch it is agglomerating do not drip, one touch i.e. dissipate for mark Standard, then the fermentation of closed 35 DEG C of constant temperature is carried out, fermentation time is 7 days;
(4)Dehydrate, step will be passed through(3)Product after fermentation is filtered, and is dried after filtering out zymotic fluid therein It is dry to be dried to obtain fermented feed.
Embodiment 3
The production technology of the cold granulation fermented feed of one boar growth promotion, comprises the following steps:
(1)The pretreatment of fermentation substrate, it would be desirable to which the raw material of fermentation are crushed first, the softening of immersion immersion afterwards 4 days;
(2)The configuration and activation of zymotic fluid, medium component is chosen, and enough honey and polypeptide are added into culture medium, and And be dissolved in glucose solution, emulsification treatment is carried out after stirring, zymocyte liquid is added thereto after emulsification, Zymocyte liquid is made up of nitrogen-fixing bacteria, Bacillus acidi lactici, leukonid, mycobacteria, red spirillum and salmonella, and process is incubated Form zymotic fluid;
(3)Fermenting raw materials, by step(1)In fermentation substrate and step(2)In fermentation broth agitation it is well mixed after add life After fermentation of materials enzyme, the biomass ferment enzyme is by lactic dehydrogenase, cellulase, hemicellulose enzyme, glutathione peroxidase At least three kinds in enzyme, mannonase zytase, b- dextranases with etc. mass ratio is well mixed forms, add thereto Clear water is added to cause water content 40%, detection specific method of the water content 40%:With hand pinch it is agglomerating do not drip, one touch i.e. dissipate for mark Standard, then the fermentation of closed 40 DEG C of constant temperature is carried out, fermentation time is 10 days;
(4)Dehydrate, step will be passed through(3)Product after fermentation is filtered, and is dried after filtering out zymotic fluid therein It is dry to be dried to obtain fermented feed.
The advantage of the invention is that:Pre-processed by the way that fermentation substrate is passed through, it is possible to increase the softening degree of matrix, can More preferable strain processing fermentation is decomposed, it is possible to increase the conversion ratio of material, and zymotic fluid individually configure and activate, The change of otherness can be carried out according to different demand, enables to fermentation substrate to obtain the processing of more preferable otherness, and It by using various enzymes during fermentation, can promote to decompose, advantageously form albumen for being easy to animal to absorb etc., and it is whole Individual technique is simple, workable, and not high for equipment requirement, can be widely popularized use.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling Change is included in the present invention.Any reference in claim should not be considered as to the involved claim of limitation.

Claims (4)

1. the production technology of the cold granulation fermented feed of a boar growth promotion, it is characterised in that comprise the following steps:
(1)The pretreatment of fermentation substrate, it would be desirable to which the raw material of fermentation are crushed first, the softening of immersion immersion afterwards 2-4 days;
(2)The configuration and activation of zymotic fluid, medium component is chosen, and enough honey and polypeptide are added into culture medium, and And be dissolved in glucose solution, emulsification treatment is carried out after stirring, zymocyte liquid is added thereto after emulsification, By incubated formation zymotic fluid;
(3)Fermenting raw materials, by step(1)In fermentation substrate and step(2)In fermentation broth agitation it is well mixed after add life After fermentation of materials enzyme, clear water is added thereto and causes water content in 30-40%, then carry out the fermentation of closed 30-40 DEG C of constant temperature, send out The ferment time is 5-10 days;
(4)Dehydrate, step will be passed through(3)Product after fermentation is filtered, and is dried after filtering out zymotic fluid therein It is dry to be dried to obtain fermented feed.
2. the production technology of the cold granulation fermented feed of boar growth promotion according to claim 1, it is characterised in that in step Suddenly(2)In, zymocyte liquid is made up of nitrogen-fixing bacteria, Bacillus acidi lactici, leukonid, mycobacteria, red spirillum and salmonella.
3. the production technology of the cold granulation fermented feed of boar growth promotion according to claim 1, it is characterised in that in step Suddenly(3)In, the biomass ferment enzyme is by lactic dehydrogenase, cellulase, hemicellulose enzyme, glutathione peroxidase, sweet Dew dextranase, zytase, at least three kinds in b- dextranases with etc. mass ratio is well mixed forms.
4. the production technology of the cold granulation fermented feed of boar growth promotion according to claim 1, it is characterised in that in step Suddenly(3)In, detection specific method of the water content in 30-40%:With hand pinch it is agglomerating do not drip, one touch i.e. dissipate for standard.
CN201710872788.9A 2017-09-25 2017-09-25 The production technology of the cold granulation fermented feed of one boar growth promotion Pending CN107373157A (en)

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Publication number Priority date Publication date Assignee Title
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CN106107064A (en) * 2016-06-27 2016-11-16 浙江绿谷牧业有限公司 The production technology of one boar growth promotion cold granulation fermented feed
CN106306349A (en) * 2015-07-01 2017-01-11 北京伟农生物科技有限公司 Food-calling puffing fermented creep feed for piggies and preparation method thereof
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CN106107064A (en) * 2016-06-27 2016-11-16 浙江绿谷牧业有限公司 The production technology of one boar growth promotion cold granulation fermented feed
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