CN106258934A - The selection of disease-resistant blue seed Semen Tritici aestivi - Google Patents

The selection of disease-resistant blue seed Semen Tritici aestivi Download PDF

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Publication number
CN106258934A
CN106258934A CN201610644282.8A CN201610644282A CN106258934A CN 106258934 A CN106258934 A CN 106258934A CN 201610644282 A CN201610644282 A CN 201610644282A CN 106258934 A CN106258934 A CN 106258934A
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disease
selection
plant
fringe
resistant
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CN106258934B (en
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王伟
何庆才
陈天青
隋建枢
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Guizhou Upland Crop Research Institute
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Guizhou Upland Crop Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses the selection of a kind of disease-resistant blue seed Semen Tritici aestivi.The present invention uses disease-resistant parent to hybridize with blue seed Semen Tritici aestivi, plant in susceptible serious plot, employing modified method of mixing selection selects, the mixing of the lowest generation selects and mixed planting, in high generation, divides fringe system to select and identifies, manyly carry out stability again after being bred as stable disease-resistant strain simultaneously, yielding ability, Disease Resistance Identification, the utilizable blue seed new strain of wheat of final incubation, the blue seed new strain of wheat being bred as is with the Wheat Yield of popularization in Guizhou Province quite (average yield per mu 280 kilograms), more original kind of matter volume increase about 40%, high stripe rust resisting, powdery mildew, suitable most area plantation in Guizhou Province.

Description

The selection of disease-resistant blue seed Semen Tritici aestivi
Technical field
The present invention relates to crops breeding technique field, the selection of a kind of disease-resistant blue seed Semen Tritici aestivi.
People increasingly pay attention to the food with natural pigment in recent years, and blue seed wheat seed epidermis is blueness, embryo Be blueness with endosperm fraction, after powder process powder because anthocyanidin content is more in green, so the food that blue seed Semen Tritici aestivi makes Product are liked by consumer very much.But owing in Guizhou Province's Wheat Production, stripe rust, powdery mildew occur serious, the initially blueness of initiative Seed Semen Tritici aestivi not stripe rust resisting, powdery mildew, and yield level is relatively low, it is difficult to directly apply in Wheat Production.
Summary of the invention
It is an object of the invention to: the selection of a kind of disease-resistant blue seed Semen Tritici aestivi, the new strain of wheat that it is bred as are provided Suitable with the Wheat Yield promoted in Guizhou Province, and high stripe rust resisting, powdery mildew, suitably most area in Guizhou Province Plantation, to overcome the deficiencies in the prior art.
The present invention is achieved in that the selection of disease-resistant blue seed Semen Tritici aestivi, uses the Semen Tritici aestivi conduct of your agriculture series Disease-resistant parent hybridizes with blue seed Semen Tritici aestivi, plants in susceptible serious plot, uses modified method of mixing selection choosing Selecting, the mixing of the lowest generation selects and mixed planting, and fringe system selected and identified high generation point, after being bred as stable disease-resistant strain, 4 with Upper different regions carry out stability, yielding ability, Disease Resistance Identification, the utilizable blue seed new strain of wheat of final incubation.
Described mixing of low generation selects to specifically refer to mixed planting,
1) planting density: 150~200 strains/m2
2) chosen period: be divided into Semen Tritici aestivi milk stage and dough stage, milk stage mainly to disease resistance, plant height, fringe type, plant type and Blade profile selects, and grain color, period of duration, ripe phase and later stage disease resistance are selected by dough stage;
3) selection principle: anti-above to stripe rust, powdery mildew, leaf rust height or in anti-above, below plant height 80cm, plant type Compact or half compact, heading stage compared with early, ear node compared with thin, well developed root system, width of blade are moderate;
4) individual plant determines: not having the combination of disease-resistant strain directly to eliminate, elected individual plant is listed by milk stage, selection Strain number, depending on colony's disease resistance, selects 10~15 strains to colony anti-and above in stripe rust of wheat, powdery mildew and leaf rust, Satisfactory individual plant is all listed by the bad colony of disease resistance, and the individual plant that milk stage is listed by dough stage checks, right Blue grain is less, late onset, period of duration too late, ripe mutually bad individual plant eliminated.
5) harvest-seed extraction: individual plant subassembly is gathered in the crops by listing, mixing threshing is provided as follow-on seed.For portion Divide F5 for the unstabilized advanced clones of above disease resistance, be also adopted by hybrid selection stable to disease resistance.
Described height generation point fringe system selects to specifically refer to qualification,
1) single-strain planting: more than 10 and dissimilar the most characteristic for separating type separation type in colony Combination, uses individual plant individually to plant and is remained by different types of material, and the individual plant blueness seed of selection is more than 60%, so After in different types of individual plant colony, select single fringe again, to accelerate the stabilized speed of different kind of material;
2) single fringe plantation: for separating the type combination less than 5 in colony, then use menu fringe individually to plant and add Selecting pure lines, each combination selection 80-100 tassel soon, 1 tassel is only selected in 1 strain, and next year broadcasts 1-2 row, eliminates indigo plant before sowing The color seed fringe system less than 80%, tassel is only selected and remain blue seed, great majority so can be avoided not stablize individual plant and account in a large number The problem on ground;The fringe system of next year plantation, identifies with economical character the stability of each fringe system at Wheat in Grain Filling Stage, right The head progeny row that economical character is good and character is stable is listed, and the stable head progeny row of same type selects 2-3, and selected fringe system is blue Seed ratio will more than 98%, for the most stable, economical character is good and type and select the different individual plant of head progeny row, continue The single fringe of continuous selection carries out next year plantation, till being bred as stable fringe system, reflects economical character again with disease resistance during results Determine once, eliminate the bad head progeny row selected.
Owing to have employed technique scheme, the present invention uses disease-resistant parent (your agriculture series) to carry out with blue seed Semen Tritici aestivi Hybridization, plants in susceptible serious plot, uses modified method of mixing selection to select, and the mixing of the lowest generation selects and hybrid Planting, fringe system selected and identified high generation point, manyly carried out stability, yielding ability, disease-resistant after being bred as stable disease-resistant strain more simultaneously Property identify, final be bred as utilizable blue seed new strain of wheat, the blue seed new strain of wheat being bred as and Guizhou Quite (average yield per mu 280 kilograms), more original kind of matter increases production about 40% to the Wheat Yield promoted inside the province, high Rust resistance Sick, powdery mildew, suitably most area plantation in Guizhou Province.
Detailed description of the invention
Embodiments of the invention: the selection of disease-resistant blue seed Semen Tritici aestivi,
Test site: Kweiyang (in the institute of Guizhou Academy of Agricultural Science), Affected It In Qianxinan Xingyi City, prestige Ning County of Bijie Prefecture.
Test period: calendar year 2001 rises and all prepares blue-grain wheat cross combination every year and breeding intermediate materials is carried out selection-breeding, Started in 2010 to be bred as stable, high yield, disease-resistant blue-grain wheat new lines, be bred as 10 to 2015 and stablized new lines, Wherein 0147 is higher with 09,197 two strain yield potentialities
Parent's preparation program: with blue-grain wheat breeding resources " the green wheat in Kweiyang " for making male parent, with to stripe rust, powdery mildew height Anti-above self-fertile or draw outward Xia Fan 22,9639, interior wheat 836, continuous wheat 367, joint swallow 970012,9504 etc. hybridize as female parent
Low generation material uses and selects Single-plant selection mixed planting, selection-breeding
Too many for low generation (F5 is for following) material separation type, and disease resistance material need to be early for strictly selecting, just having can The feature of the good advanced clones of disease resistance can be bred as, so the selection-breeding of low generation material is mainly based on disease resistance, in conjunction with need The economical characters such as plant height, fringe type, plant type, blade profile, period of duration select.
(1) planting density: try one's best thin planting in field, 150~200 strains/m2It is advisable, to facilitate later observations, to identify, select.
(2) chosen period: be divided into Semen Tritici aestivi milk stage and dough stage, milk stage mainly to disease resistance, plant height, fringe type, plant type, The economical characters such as blade profile select, and grain color, period of duration, ripe phase and later stage disease resistance are selected by dough stage.
(3) selection principle: be to resist above, plant height 80cm more than stripe rust, powdery mildew, leaf rust height are resisted and in leaf rust Below, plant type is compact or half compact, heading stage compared with early, ear node compared with thin, well developed root system, width of blade are moderate.
(4) individual plant determines: not having the combination of disease-resistant strain directly to eliminate, elected individual plant is listed by milk stage, selects Strain number depending on colony's disease resistance, the colony that general disease resistance is good is advisable in 10~15 strains, and the bad colony of disease resistance is to symbol Closing the individual plant required all to list, the individual plant that milk stage is listed by dough stage checks, late onset less to blue grain, life Educate the phase too late, ripe mutually bad individual plant eliminated.
(5) harvest-seed extraction: individual plant subassembly is gathered in the crops by listing, mixing threshing is provided as follow-on seed.For Part F5, for the unstabilized advanced clones of above disease resistance, is also adopted by hybrid selection stable to disease resistance.
Advanced clones uses and selects individual plant, single independent planting method of fringe
Tend towards stability for colony's disease resistance of later most of advanced clones for F5, if continuing to use mixing to select Then it is unfavorable for the separation of different wheat types, has slowed down and be bred as stablizing the paces of strain, the emphasis that the most this kind of material selects It is that different types of material separates in colony, therefore just uses menu strain, single independent planting method of fringe.
(1) single-strain planting: for separating Class1 more than 0 kind and the most characteristic dissimilar combination in colony, use single Strain is individually planted and can be remained by different types of material, but the individual plant blueness seed selected is more than 60%, the most again In different types of individual plant colony, select single fringe, the stabilized speed of different kind of material can be accelerated, but use this selection Method material should be little, otherwise can strengthen breeding work difficulty and workload.
(2) single fringe plantation: group types is less than to the combination of 5, then use menu fringe individually to plant and accelerate to select Being sheerly, each combination selection 80~100 tassels, 1 tassel is only selected in 1 strain, and next year broadcasts 1-2 row, eliminates blue seed before sowing The grain fringe system less than 80%, the blue seed so tassel is only selected and remain, great majority so can be avoided not stablize individual plant and to account in a large number The problem on ground.The fringe system of next year plantation, identifies with economical character the stability of each fringe system at Wheat in Grain Filling Stage, right The head progeny row that economical character is good and character is stable is listed, and the stable head progeny row of same general type selects 2~3 and is advisable, selected Fringe system blueness seed ratio will more than 98%, for the most stable, economical character is good and type differs with selecting head progeny row The individual plant of sample, continues to select single fringe to carry out next year plantation, till being bred as stable fringe system, during results to economical character with Disease resistance is identified once again, eliminates the bad head progeny row that selected, the combination through the general good stability of this method can choose 3~ 5 stable head progeny rows become new lines.
(3) stability and Appraisal of The High Yield Property;To the blue seed new strain of wheat being bred as mainly to its stability, disease-resistant Property, yielding ability identifies, and using local spread kind as comparison, is important to enter the adaptability of planted in different ecological areas Row is investigated, and qualification result can make reference as participating in regional testing in different regions, which adds different blue Semen Tritici aestivi The new lines probability by Regional Trial of Wheat Cultivars.

Claims (3)

1. the selection of a disease-resistant blue seed Semen Tritici aestivi, it is characterised in that: use the Semen Tritici aestivi of your agriculture series as disease-resistant parent This hybridizes with blue seed Semen Tritici aestivi, plants in susceptible serious plot, uses modified method of mixing selection to select, the lowest Generation mixing selects and mixed planting, and height generation point fringe system selects and qualification, after being bred as stable disease-resistant strain, different more than 4 Area carries out stability, yielding ability, Disease Resistance Identification, the utilizable blue seed new strain of wheat of final incubation.
The selection of disease-resistant blue seed Semen Tritici aestivi the most according to claim 1, it is characterised in that: described mixing of low generation Select to specifically refer to mixed planting,
1) planting density: 150~200 strains/m2
2) chosen period: be divided into Semen Tritici aestivi milk stage and dough stage, milk stage, is mainly to disease resistance, plant height, fringe type, plant type and blade profile Selecting, grain color, period of duration, ripe phase and later stage disease resistance are selected by dough stage;
3) selection principle: anti-above to stripe rust, powdery mildew, leaf rust height or in anti-above, below plant height 80cm, plant type are compact Or half compact, heading stage compared with early, ear node compared with thin, well developed root system, width of blade are moderate;
4) individual plant determines: not having the combination of disease-resistant strain directly to eliminate, elected individual plant is listed by milk stage, the strain number of selection Depending on colony's disease resistance, colony anti-and above in stripe rust of wheat, powdery mildew and leaf rust is selected 10~15 strains, disease-resistant Property bad colony satisfactory individual plant is all listed, the individual plant that milk stage is listed by dough stage checks, to blue grain Less, late onset, period of duration are too late, ripe mutually bad individual plant eliminated.
5) harvest-seed extraction: individual plant subassembly is gathered in the crops by listing, mixing threshing is provided as follow-on seed.For part F5 For the unstabilized advanced clones of above disease resistance, it is also adopted by hybrid selection stable to disease resistance.
The selection of disease-resistant blue seed Semen Tritici aestivi the most according to claim 1, it is characterised in that: described height generation point fringe System selects to specifically refer to qualification,
1) single-strain planting: for separation type separation type in colony more than 10, and the most characteristic dissimilar combination, Using individual plant individually to plant to be remained by different types of material, the individual plant blueness seed of selection is more than 60%, the most again Single fringe is selected, to accelerate the stabilized speed of different kind of material in different types of individual plant colony;
2) single fringe plantation: for separating the type combination less than 5 in colony, then use menu fringe individually to plant and accelerate choosing Going out pure lines, each combination selection 80-100 tassel, 1 tassel is only selected in 1 strain, and next year broadcasts 1-2 row, eliminates blue seed before sowing The grain fringe system less than 80%, tassel is only selected and remain blue seed, and great majority so can be avoided not stablize what individual plant took up an area in a large number Problem;The fringe system of next year plantation, identifies with economical character, to agronomy the stability of each fringe system at Wheat in Grain Filling Stage The head progeny row that character is good and character is stable is listed, and the stable head progeny row of same type selects 2-3, selected fringe system blueness seed Ratio will more than 98%, for the most stable, economical character is good and type with selected the different individual plant of head progeny row, continue to select Select single fringe and carry out next year plantation, till being bred as stable fringe system, during results, economical character and disease resistance are identified one again Secondary, eliminate the bad head progeny row selected.
CN201610644282.8A 2016-08-09 2016-08-09 The selection of disease-resistant blue seed wheat Active CN106258934B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106901172A (en) * 2017-03-16 2017-06-30 贵州省旱粮研究所 Fresh root of kudzu vine selenium-rich green noodle and preparation method thereof
CN110150140A (en) * 2019-06-27 2019-08-23 重庆市农业科学院 A kind of Waxy wheat Rapid identification and selection
CN114365688A (en) * 2022-01-25 2022-04-19 四川省农业科学院作物研究所 Breeding method for new wheat variety

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Publication number Priority date Publication date Assignee Title
WO2002059269A2 (en) * 2001-01-04 2002-08-01 Yeda Research And Development Co. Ltd. Maintenance of genic male-sterile female parental lines using a maintainer line with a stable engineered chromosome
CN1826875A (en) * 2006-04-06 2006-09-06 西北农林科技大学 Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character
CN101755668A (en) * 2008-12-24 2010-06-30 周扬家 Breeding method of purplish black waxy wheat

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002059269A2 (en) * 2001-01-04 2002-08-01 Yeda Research And Development Co. Ltd. Maintenance of genic male-sterile female parental lines using a maintainer line with a stable engineered chromosome
CN1826875A (en) * 2006-04-06 2006-09-06 西北农林科技大学 Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character
CN101755668A (en) * 2008-12-24 2010-06-30 周扬家 Breeding method of purplish black waxy wheat

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106901172A (en) * 2017-03-16 2017-06-30 贵州省旱粮研究所 Fresh root of kudzu vine selenium-rich green noodle and preparation method thereof
CN110150140A (en) * 2019-06-27 2019-08-23 重庆市农业科学院 A kind of Waxy wheat Rapid identification and selection
CN110150140B (en) * 2019-06-27 2022-03-01 重庆市农业科学院 Method for quickly identifying and breeding waxy wheat
CN114365688A (en) * 2022-01-25 2022-04-19 四川省农业科学院作物研究所 Breeding method for new wheat variety

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