CN106258479A - A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers. - Google Patents
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers. Download PDFInfo
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- CN106258479A CN106258479A CN201610633689.0A CN201610633689A CN106258479A CN 106258479 A CN106258479 A CN 106258479A CN 201610633689 A CN201610633689 A CN 201610633689A CN 106258479 A CN106258479 A CN 106258479A
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- hericium erinaceus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention principally falls into Hericium erinaceus culture technical field, is specifically related to a kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers..Described method is the cultivation management of Hericium erinaceus (Bull. Ex Fr.) Pers. in hericium erinaceus fruiting body formation stages, described method includes prepared by culture medium for cultivating, sterilizing, cooling, inoculation and hericium erinaceus fruiting body incubation step, described culture medium for cultivating preparation process is: first with protease, bacterium glass is carried out enzymolysis, then in bacterium glass after enzymolysis, lignin regulator is added, a great number of elements supplement, exoenzyme derivant, nitrogen source supplementing agent and carbon source supplement, mix homogeneously obtains compound, calcium superphosphate is added to regulate pH to 5.5 6 in described compound, compound after reconciling pH carries out pile and becomes thoroughly decomposed, prepare the culture medium for cultivating for cultivating Hericium erinaceus (Bull. Ex Fr.) Pers..In the method for the invention, the nutritional labeling of the culture medium for cultivating prepared is to design according to the growth characteristics of Hericium erinaceus (Bull. Ex Fr.) Pers. specially, has specific aim, can improve the upgrowth situation of high Hericium erinaceus (Bull. Ex Fr.) Pers..
Description
Technical field
The present invention principally falls into Hericium erinaceus culture technical field, is specifically related to a kind of side utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers.
Method.
Background technology
Hericium erinaceus (Bull. Ex Fr.) Pers. is a kind of macro fungi, and Hydnum erinaceus sporophore is spherical in shape, be covered with above the same needle-like bacterium thorn of hair (also known as
Bacterium sends out).Like the head of little monkey, referred to as Hericium erinaceus (Bull. Ex Fr.) Pers..Hericium erinaceus (Bull. Ex Fr.) Pers. is the food of a kind of top grade, the dual-purpose bacterium of medicine.Hericium erinaceus (Bull. Ex Fr.) Pers. nutrition is rich
Richness, containing up to 17 kinds of aminoacid, wherein needed by human body account for 8 kinds.Every fatty 4.2 grams of hectogram Hericium erinaceus (Bull. Ex Fr.) Pers., is qualified
High protein, low-fat food, the most also rich in various vitamin and inorganic salt.Hericium erinaceus (Bull. Ex Fr.) Pers. has appetite stimulator, strengthens gastric mucosa
Barrier function, improves lymhocyte transformation rate, promotes the effects such as leukocyte.Therefore human body can be made to improve the immunity energy to disease
Power.Hedgehog hydnum or good nourishing food, have good efficacy to neurasthenia, digestive tract ulcer.In screening anticancer medicine, send out
Existing its has obvious anticancer function to skin, muscle cancerous protuberance.
According to the difference of cultivation needed raw material, edible fungi can be divided into straw rotting fungus and domestomycetes, rotten type Edible Fungi with
The wood flour of broad leaf tree is base stock, and Hericium erinaceus (Bull. Ex Fr.) Pers. belongs to the one of domestomycetes.In the prior art, the culture medium multiselect of Hericium erinaceus (Bull. Ex Fr.) Pers.
With Quercus acutissima, cork oak, the blue or green just seeds wood flour such as oak, live oak, and cotton seed hulls is as carbon source；Typically do not use wheat straw, Caulis et Folium Oryzae class
Herbaceous plant is as raw material, and a bacterium time of Hericium erinaceus (Bull. Ex Fr.) Pers. and fruiting time are longer；As forage, wheat straw, Caulis et Folium Oryzae class draft are planted
The composition of thing mostly is cellulose, and content of lignin is on the low side, it is difficult to is decomposed for a long time by mycelia and keeps basic purseful shape, therefore as far as possible
Straw rotting fungus class is produced with it.
Along with the fast development of Edible Fungi, the demand of hardwood sawdust is increased year by year.According to rough measuring and calculating, often
Produce 1000 bags of domestomycetes and about consume broad leaf tree timber 1m3.Edible Fungi consumes the forest reserves and in a large number to broad-leaf forest resource
Protection build-up of pressure, thus between bacterium industry, forestry, produced contradiction is i.e. " bacterium woods contradiction ".
Bacterium glass refers to the growth of microorganism needs such as nutrition edibility bacterium, medicinal fungus, and Solar use efficiency is high, has
The herbaceous plant that comprehensive development and utilization is worth, bacterium glass feature is the nutrition abundant containing protein, phosphorus, potassium, magnesium, calcium etc., is suitable for
The needs of the growth of food/medicinal fungus.
At present, utilize bacterium glass to prepare in the technology of culture medium of edible fungus, be not specifically designed for the growth characteristics of Hericium erinaceus (Bull. Ex Fr.) Pers.
It is prepared the technical scheme of culture medium.
Summary of the invention
For the problems referred to above, the present invention provides a kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., and described method is to cultivation training
Support base preparation process be optimized, utilize bacterium glass for primary raw material, and add lignin regulator, a great number of elements supplement,
Nitrogen source supplementing agent and carbon source supplement, the nutritional labeling of the culture medium for cultivating prepared is that the special growth according to Hericium erinaceus (Bull. Ex Fr.) Pers. is special
Property design, there is specific aim, the upgrowth situation of high Hericium erinaceus (Bull. Ex Fr.) Pers. can be improved.
The present invention is achieved by the following technical solutions:
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., described method is Hericium erinaceus (Bull. Ex Fr.) Pers. in hericium erinaceus fruiting body formation stages
Cultivation management, described method includes that culture medium for cultivating is prepared, sterilizing, cooled down, inoculates and hericium erinaceus fruiting body incubation step,
Described culture medium for cultivating preparation process is: first with protease bacterium glass carries out enzymolysis, then adds in bacterium glass after enzymolysis
Lignin regulator, a great number of elements supplement, exoenzyme derivant, nitrogen source supplementing agent and carbon source supplement, mix homogeneously obtains
Compound, in described compound, interpolation calcium superphosphate is to regulate pH to 5.5-6, and it is rotten that the compound after reconciling pH carries out pile
Ripe, prepare the culture medium for cultivating for cultivating Hericium erinaceus (Bull. Ex Fr.) Pers..
Further, by weight, the described culture medium for cultivating prepared includes the component of following weight portion: enzymolysis
Rear bacterium glass 50-60 part；Lignin regulator 20-30 part；A great number of elements supplement 2-3 part, nitrogen source supplementing agent 5-10 part, carbon source are mended
Fill agent 5-10 part, exoenzyme derivant 0.5-1 part, calcium superphosphate 3-5 part.
Further, bacterium glass being carried out enzymolysis particularly as follows: pulverized by bacterium glass, after controlling to pulverize, bacterium glass length range is 0.5-
2cm, is placed in bacterium glass in enzymatic vessel, then according to add the ratio of 200-300mL protein enzyme solution in per kilogram bacterium glass toward enzyme
Solving and add protein enzyme solution in tank, controlled enzymatic hydrolysis condition is: hydrolysis temperature 30-50 DEG C, enzymolysis time: 12-20h, enzyme addition:
500IU/g bacterium glass.Owing to the protein in bacterium glass can not directly be utilized by Hericium erinaceus (Bull. Ex Fr.) Pers., easily cause the waste in nitrogen source, at this
Utilizing protease that the protein digestion in bacterium glass is become aminoacid in invention, aminoacid is the utilizable a kind of nitrogen of Hericium erinaceus (Bull. Ex Fr.) Pers.
Source, solves the drawback that in bacterium glass, protein can not be fully utilized.Wherein bacterium glass select Dicranopteris dichotoma, Plantula Neyraudiae Reynaudianae, Radix sacchari arundinacei, Caulis Miscanthis floriduli,
Any one or two or more group in phragmites communis, Arundo donax, grassiness, Jujun grasses, Sorghum propinquum, alfalfa, arabian cron, Lay bamboo
Close.
Further, the carbon-nitrogen ratio controlling described compound is 35-45:1.
The concrete process that controls is by bacterium glass, lignin regulator, nitrogen source supplementing agent and carbon source supplement after regulation enzymolysis
Addition regulate and control, the carbon-nitrogen ratio finally making compound is 35-45:1.Why controlling compound carbon-nitrogen ratio is 35-45:
1, the optimum carbon nitrogen ratio in the fruit-body formation stage that reason is Hericium erinaceus (Bull. Ex Fr.) Pers. is 35-45:1, so preparing at culture medium for cultivating
Cheng Zhong, the carbon-nitrogen ratio controlling compound is 35-45:1, is to determine for the growth characteristics of Hericium erinaceus (Bull. Ex Fr.) Pers., time too high or too low,
All it is unfavorable for the growth of Hericium erinaceus (Bull. Ex Fr.) Pers..
Further, the preparation of described lignin regulator is particularly as follows: jade after processing with Pericarppium arachidis hypogaeae, cotton seed hulls and dry in the sun
Any one in rice core furfural dregs is raw material, grinds, and crosses 40 mesh sieves, i.e. prepares described lignin regulator；System
The content of lignin of the raw material that standby lignin regulator is used is above 25%, the wherein weight percent of lignin in Pericarppium arachidis hypogaeae
Ratio is 29-32% for the percentage by weight of lignin in 27-29%, cotton seed hulls, lignin in the furfural dregs after dry in the sun process
Percentage by weight is 28-30%.By the addition of lignin regulator, solve bacterium glass as content of lignin in herbaceous plant
The low drawback being not suitable for cultivating Hericium erinaceus (Bull. Ex Fr.) Pers., wherein, it is necessary to the strict addition controlling lignin regulator, if addition is too low,
In the culture medium for cultivating obtained, content of lignin is too low and cause Hericium erinaceus (Bull. Ex Fr.) Pers. growth conditions the best, it is impossible to enough keep basic purseful
Shape.
Further, a great number of elements supplement include potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium sulfate, magnesium sulfate.In a large number
Component extender is except can providing a great number of elements phosphorus, potassium, Calcium Magnesium Sulphur, additionally it is possible to as buffer agent, make the pH of compost
Value maintains suitable scope.
Further, any one in the mistake Testa Tritici of 60 mesh sieves, Testa oryzae and soybean stalk after nitrogen source supplementing agent is pulverizing；
The nitrogen content of Testa Tritici, Testa oryzae and soybean stalk is higher, prepares culture medium for cultivating again, it is possible to preferably by monkey after being pulverized and sieved
Mushroom utilizes head.
Further, carbon source supplement include any one in sucrose, white sugar and starch, in carbon source supplement
Sucrose, white sugar and starch are all quick-acting nutrients, quickly can be utilized by mycelium, so adding in culture medium for cultivating
Carbon source supplement, can promote that mycelia mushrooms out.
Further, the outer derivant of described born of the same parents is glucose, and glucose can induce exoenzyme as exoenzyme derivant
Generation, thus promote the decomposition of cellulose.
Further, compound is carried out pile and becomes thoroughly decomposed particularly as follows: the compound after reconciling pH carries out pile, control pile
The moisture of rear stockpile is 40-50%, plastic covering film above stockpile, carries out insulation and becomes thoroughly decomposed, turning one in every 5 days
Secondary, when the temperature in described stockpile reaches 45-65 DEG C, carry out turning, the pile time of becoming thoroughly decomposed is 15-20 days, controls pile rotten
The pH of the raw material after ripe is 5.0～5.5.During pile, the pH of compound can reduce about 0.5, so to mixing stockpile
Before code becomes thoroughly decomposed, need the pH of compound is controlled；In view of the optimum pH of hericium erinaceus fruiting body growth stage be 5.0～
5.5, when regulating compound pH, controlling compound pH is 5.5-6.
The Advantageous Effects of the present invention:
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers. that the present invention provides, utilizes in the preparation process of culture medium for cultivating
Bacterium glass is primary raw material, alleviates " bacterium woods contradiction "；And producing aminoacid by protein in bacterium glass being carried out enzymolysis, making monkey
Head mushroom can more make full use of rich in protein in bacterium glass.
Meanwhile, in the present invention by adding lignin regulator, a great number of elements supplement, exoenzyme derivant, nitrogen source
Supplement and carbon source supplement, the nutritional labeling of the culture medium for cultivating prepared is to set according to the growth characteristics of Hericium erinaceus (Bull. Ex Fr.) Pers. specially
Meter, has specific aim, can improve the upgrowth situation of high Hericium erinaceus (Bull. Ex Fr.) Pers..
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, to the present invention
It is explained in further detail.Should be appreciated that specific embodiment described herein is used only for explaining the present invention, be not used to
Limit the present invention.
On the contrary, the present invention contain any be defined by the claims the replacement done in the spirit and scope of the present invention, repair
Change, equivalent method and scheme.Further, in order to make the public that the present invention to be had a better understanding, thin to the present invention below
During joint describes, detailed describe some specific detail sections.There is no these detail sections for a person skilled in the art
Description can also understand the present invention completely.
Embodiment 1
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., described method is Hericium erinaceus (Bull. Ex Fr.) Pers. in hericium erinaceus fruiting body formation stages
Cultivation management, described method includes that culture medium for cultivating is prepared, sterilizing, cooled down, inoculates and hericium erinaceus fruiting body incubation step,
Described culture medium for cultivating preparation process is:
(1) utilize protease that bacterium glass carries out enzymolysis:
(2) toward adding lignin regulator in bacterium glass after enzymolysis, a great number of elements supplement, exoenzyme derivant, nitrogen source are mended
Filling agent and carbon source supplement, mix homogeneously obtains compound,
(3) in described compound, interpolation calcium superphosphate is to regulate pH to 6, and it is rotten that the compound after reconciling pH carries out pile
Ripe, prepare the culture medium for cultivating for cultivating Hericium erinaceus (Bull. Ex Fr.) Pers..
By weight, the described culture medium for cultivating prepared includes the component of following weight portion: bacterium glass 50 after enzymolysis
Part；Lignin regulator 20 parts；A great number of elements supplement 2 parts, nitrogen source supplementing agent 5 parts, carbon source supplement 5 parts, exoenzyme are induced
Agent 0.5 part, calcium superphosphate 3 parts.
In step (1), bacterium glass being carried out enzymolysis particularly as follows: pulverized by bacterium glass, after controlling to pulverize, bacterium glass length range is
0.5cm, is placed in bacterium glass in enzymatic vessel, then according to add the ratio of 200mL protein enzyme solution in per kilogram bacterium glass toward enzymolysis
Adding protein enzyme solution in tank, controlled enzymatic hydrolysis condition is: hydrolysis temperature 40 DEG C, enzymolysis time: 18h, enzyme addition: 500IU/g
Bacterium glass.
The carbon-nitrogen ratio controlling described compound is 45:1.
The preparation of described lignin regulator, particularly as follows: with Pericarppium arachidis hypogaeae as raw material, grind, is crossed 40 mesh sieves, is i.e. prepared
Obtain described lignin regulator；
A great number of elements supplement include potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium sulfate, magnesium sulfate.Potassium dihydrogen phosphate, phosphorus
Acid hydrogen dipotassium, calcium sulfate, the ratio of magnesium sulfate are 1:1:1:1.
Nitrogen source supplementing agent be pulverize after cross in the Testa Tritici of 60 mesh sieves, Testa oryzae and soybean stalk any one.
Carbon source supplement are sucrose；The outer derivant of described born of the same parents is glucose.
Compound carries out pile become thoroughly decomposed particularly as follows: the compound after reconciling pH carries out pile, control stockpile after pile
Moisture be 50%, plastic covering film above stockpile, carry out insulation and become thoroughly decomposed, turning in every 5 days once, when described former
When temperature in stockpile reaches 45-65 DEG C, carrying out turning, the pile time of becoming thoroughly decomposed is 15 days, controls the pH of the raw material after pile becomes thoroughly decomposed
5.5.
Embodiment 2
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., described method is Hericium erinaceus (Bull. Ex Fr.) Pers. in hericium erinaceus fruiting body formation stages
Cultivation management, described method includes that culture medium for cultivating is prepared, sterilizing, cooled down, inoculates and hericium erinaceus fruiting body incubation step,
Described culture medium for cultivating preparation process is:
(1) utilize protease that bacterium glass carries out enzymolysis:
(2) toward adding lignin regulator in bacterium glass after enzymolysis, a great number of elements supplement, exoenzyme derivant, nitrogen source are mended
Filling agent and carbon source supplement, mix homogeneously obtains compound,
(3) in described compound, interpolation calcium superphosphate is to regulate pH to 5.5, and the compound after reconciling pH carries out pile
Become thoroughly decomposed, prepare the culture medium for cultivating for cultivating Hericium erinaceus (Bull. Ex Fr.) Pers..
By weight, the described culture medium for cultivating prepared includes the component of following weight portion: bacterium glass 55 after enzymolysis
Part；Lignin regulator 25 parts；A great number of elements supplement 2.5 parts, nitrogen source supplementing agent 8 parts, carbon source supplement 9 parts, exoenzyme lure
Lead agent 0.7 part, calcium superphosphate 4 parts.
Bacterium glass being carried out enzymolysis particularly as follows: pulverized by bacterium glass, after controlling to pulverize, bacterium glass length range is 0.5-2cm, by bacterium
Grass is placed in enzymatic vessel, then according to the ratio adding 250mL protein enzyme solution in per kilogram bacterium glass adds egg in enzymatic vessel
White enzymatic solution, controlled enzymatic hydrolysis condition is: hydrolysis temperature 35 DEG C, enzymolysis time: 18h, enzyme addition: 500IU/g bacterium glass.
The carbon-nitrogen ratio controlling described compound is 35-45:1.
Described lignin regulator preparation particularly as follows: with dry in the sun process after corn cob furfural dregs raw material, grind,
Cross 40 mesh sieves, i.e. prepare described lignin regulator；
A great number of elements supplement include potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium sulfate, magnesium sulfate.Potassium dihydrogen phosphate, phosphorus
Acid hydrogen dipotassium, calcium sulfate, the ratio of magnesium sulfate are 1:1:1:1.
Nitrogen source supplementing agent be pulverize after cross 60 mesh sieves Testa Tritici；Carbon source supplement are white sugar；The outer derivant of described born of the same parents is Portugal
Grape sugar.
Compound carries out pile become thoroughly decomposed particularly as follows: the compound after reconciling pH carries out pile, control stockpile after pile
Moisture be 40%, plastic covering film above stockpile, carry out insulation and become thoroughly decomposed, turning in every 5 days once, when described former
When temperature in stockpile reaches 45 DEG C, carrying out turning, the pile time of becoming thoroughly decomposed is 15 days, and the pH controlling the raw material after pile becomes thoroughly decomposed exists
5.0。
Claims (10)
1. the method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., described method is Hericium erinaceus (Bull. Ex Fr.) Pers. in hericium erinaceus fruiting body formation stages
Cultivation management, described method includes that culture medium for cultivating is prepared, sterilizing, cooled down, inoculates and hericium erinaceus fruiting body incubation step, its
Being characterised by, described culture medium for cultivating preparation process is: first with protease bacterium glass carries out enzymolysis, then toward bacterium after enzymolysis
Grass adds lignin regulator, a great number of elements supplement, exoenzyme derivant, nitrogen source supplementing agent and carbon source supplement, mixing
Uniformly obtaining compound, in described compound, interpolation calcium superphosphate is to regulate pH to 5.5-6, and the compound after reconciling pH enters
Windrow code becomes thoroughly decomposed, and prepares the culture medium for cultivating for cultivating Hericium erinaceus (Bull. Ex Fr.) Pers..
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., it is characterised in that by weight, system
The standby described culture medium for cultivating obtained includes the component of following weight portion: bacterium glass 50-60 part after enzymolysis；Lignin regulator 20-
30 parts；A great number of elements supplement 2-3 part, nitrogen source supplementing agent 5-10 part, carbon source supplement 5-10 part, exoenzyme derivant 0.5-1
Part, calcium superphosphate 3-5 part.
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., it is characterised in that bacterium glass is carried out enzymolysis
Particularly as follows: pulverized by bacterium glass, after controlling to pulverize, bacterium glass length range is 0.5-2cm, is placed in enzymatic vessel by bacterium glass, then according to
The ratio adding 200-300mL protein enzyme solution in per kilogram bacterium glass adds protein enzyme solution, controlled enzymatic hydrolysis bar in enzymatic vessel
Part is: hydrolysis temperature 30-50 DEG C, enzymolysis time: 12-20h, enzyme addition: 500IU/g bacterium glass.
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., it is characterised in that control described compound
Carbon-nitrogen ratio be 35-45:1.
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., it is characterised in that described lignin regulates
The preparation of agent is particularly as follows: with any one in the corn cob furfural dregs after Pericarppium arachidis hypogaeae, cotton seed hulls and dry in the sun process as raw material, grind
Pulverizing is broken, crosses 40 mesh sieves, i.e. prepares described lignin regulator；The percent mass of lignin in described lignin regulator
Ratio is higher than 25%.
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., it is characterised in that a great number of elements supplement
Including potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium sulfate, magnesium sulfate.
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., it is characterised in that nitrogen source supplementing agent is powder
Any one in the mistake Testa Tritici of 60 mesh sieves, Testa oryzae and soybean stalk after broken.
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., it is characterised in that carbon source supplement include
Any one in sucrose, white sugar and starch.
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., it is characterised in that the outer derivant of described born of the same parents
For glucose.
A kind of method utilizing bacterium glass cultivation Hericium erinaceus (Bull. Ex Fr.) Pers., it is characterised in that compound is carried out
Pile becomes thoroughly decomposed particularly as follows: the compound after reconciling pH carries out pile, and after control pile, the moisture of stockpile is 40-50%,
Plastic covering film above stockpile, carries out insulation and becomes thoroughly decomposed, turning in every 5 days once, when the temperature in described stockpile reaches 45-
When 65 DEG C, carrying out turning, the pile time of becoming thoroughly decomposed is 15-20 days, controls the pH of the raw material after pile becomes thoroughly decomposed 5.0～5.5.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108012758A (en) * | 2017-12-05 | 2018-05-11 | 罗成喜 | A kind of cultural method of Hericium erinaceus |
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| CN1081059A (en) * | 1992-07-07 | 1994-01-26 | 福建农学院食用菌实验场 | Improve the method for cultivating adible mushroom of fungus grass culturing material nutrient |
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| CN102329171A (en) * | 2011-07-15 | 2012-01-25 | 安徽省庆元堂徽菊有限公司 | Culture base material for cultivating shiitake by using chrysanthemum straws and method for cultivating shiitake by using culture base material |
| WO2015180624A1 (en) * | 2014-05-27 | 2015-12-03 | Novozymes A/S | Methods for mushroom cultivation |
| CN105237126A (en) * | 2015-08-27 | 2016-01-13 | 马鞍山市安康菌业有限公司 | Pennisetum giganteum and mushroom bran dictyophora high-effective culture medium and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1081059A (en) * | 1992-07-07 | 1994-01-26 | 福建农学院食用菌实验场 | Improve the method for cultivating adible mushroom of fungus grass culturing material nutrient |
| CN101665373A (en) * | 2009-09-28 | 2010-03-10 | 王光文 | Safe and high-efficiency preparation method of master culture medium material of edible fungi |
| CN102329171A (en) * | 2011-07-15 | 2012-01-25 | 安徽省庆元堂徽菊有限公司 | Culture base material for cultivating shiitake by using chrysanthemum straws and method for cultivating shiitake by using culture base material |
| WO2015180624A1 (en) * | 2014-05-27 | 2015-12-03 | Novozymes A/S | Methods for mushroom cultivation |
| CN105237126A (en) * | 2015-08-27 | 2016-01-13 | 马鞍山市安康菌业有限公司 | Pennisetum giganteum and mushroom bran dictyophora high-effective culture medium and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108012758A (en) * | 2017-12-05 | 2018-05-11 | 罗成喜 | A kind of cultural method of Hericium erinaceus |
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Application publication date: 20170104 |