CN106258370A - Simple Fructus Fragariae Ananssae virus-elimination seedlings mating system - Google Patents
Simple Fructus Fragariae Ananssae virus-elimination seedlings mating system Download PDFInfo
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- CN106258370A CN106258370A CN201610659584.2A CN201610659584A CN106258370A CN 106258370 A CN106258370 A CN 106258370A CN 201610659584 A CN201610659584 A CN 201610659584A CN 106258370 A CN106258370 A CN 106258370A
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- seedling
- original seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention belongs to gardening strawberry seedling breeding technical field, disclosing a kind of simple Fructus Fragariae Ananssae virus-elimination seedlings mating system, the steps such as pot transplanting (4) promotes the arrangement of original seed Seedling stolon pumping (5) seedling fixing are changed in acquisition (2) nursery preparation (3) including (1) detoxification original seed Seedling.The present invention reduces Fructus Fragariae Ananssae virus-elimination seedlings raising technology, is effectively improved the safety of kind.The method is easy and simple to handle, easily grasps, and repeatability is strong, breaks through Routine Test Lab biology raising technology, reduces labour force, reduces material cost, avoids again open country to breed the risk that pest and disease damage brings.
Description
Technical field
The present invention relates to a kind of simple Fructus Fragariae Ananssae virus-elimination seedlings mating system, belong to strawberry seedling breeding technical field.
Background technology
Fructus Fragariae Ananssae is important berry tree, and fruit appearance is bright-coloured, and unique flavor is of high nutritive value, and purposes is wide, have
The good reputation of " fruit queen "；It is with short production cycle, instant effect, income high, is at home and abroad widely cultivated, and it cultivates face
Long-pending and yield occupy the first in world's small berries produces always.Show according to the World Food Programme (FAO) statistics: 2013
Whole world cultivated strawberry area is 542.49 ten thousand mu, and yield reaches 773.96 ten thousand tons；Wherein, the cultivated area of China Fructus Fragariae Ananssae is with total
Yield is respectively 164.91 ten thousand mu and 299.75 ten thousand tons, the Ge Zhan world Fructus Fragariae Ananssae gross area and 30.40% He of total output
38.73%, occupy the first in the world in pairs.
China needs more than detoxification original seed Seedling 1.6 hundred million strain every year, and the former seedling year of China's detoxification produces about according to incompletely statistics
0.15 hundred million strains, the market has openings of detoxification original seed Seedling reaches 98%.Current domestic Fructus Fragariae Ananssae detoxification original seed Seedling mainly utilizes biotechnology
Tissue-culturing rapid propagation, technology requires that difficulty is grasped, is difficult to popularize, limited amount system, can not meet far away the market demand.In addition Fructus Fragariae Ananssae is nothing
Sexual reproduction, breeds market confusion, and detoxification original seed Seedling, virus-elimination seedlings, common seedling and production Seedling are confused, and seedling is numerous in addition
Educating backward in technique, cause variety deterioration, pathogenic bacteria rate is high, of poor quality, therefore cultivates the virus-elimination seedlings of healthy and strong high-quality, cracks restriction grass
The countermeasure of certain kind of berries industry development bottleneck is penetration and promotion virus-elimination seedlings raising technology.
Summary of the invention
The object of the invention is for the bottleneck of restriction Strawberry industry development, and purpose provides a kind of simple Fructus Fragariae Ananssae virus-elimination seedlings to breed
Method.
For realizing above-mentioned target, the invention provides a kind of simple Fructus Fragariae Ananssae virus-elimination seedlings mating system, its step includes:
(1) acquisition of detoxification original seed Seedling: the stolon stem apex that April, clip from plant newly grew, flowing water rinses, disappears
Poison, inoculation, grow 25 ± 2 days and obtain aseptic seedling；Take above-mentioned aseptic seedling subculture multiplication, rooting and transplant promotees to live, and grows and obtains for 70 ± 8 days
Obtain detoxification original seed Seedling；
(2) nursery prepares:
2.1) addressing, whole: the address challenges of selection is away from Fructus Fragariae Ananssae, the paddy field of vegetable solanaceous vegetables, ground grading, and hole tray is filled out
Filling and tamp tiling earth's surface after substrate, make hole tray edge be slightly below surrounding soil, irrigation and drainage are convenient, by reserved detoxification original seed Seedling position
With management passage；
2.2) booth is built: on nursery, build booth, it is desirable to winter can be incubated, insect protected of taking shelter from rain summer, ventilation and penetrating light,
When illumination is strong, can add a cover sunshade net protection, simultaneously install automatic micro-sprinkling irrigation facility, can micro-spray repair water, can precision fertilizing；
2.3) first 20 days of field planting, substrate being watered foot water, to boil in a covered pot over a slow fire canopy high-temperature sterilization aeration-cooling to field planting front opening；
(3) pot transplanting field planting is changed: late July, by seedling exercising in the original seed rearmounted booth of Seedling detoxification, moves after 1 week of centralized management
Planting, detoxification original seed Seedling uses potted plant mode, and basin mouth edge is concordant with hole tray, and the most reserved hole tray breeds seedling, and Routine Management is fertile
Water, booth requires ventilation and penetrating light, when illumination is strong, adds a cover sunshade net protection；
(4) detoxification original seed Seedling stolon pumping is promoted: use suitable management mode to promote detoxification original seed Seedling stolon
Pumping；
(5) seedling arranges fixing: after detoxification original seed Seedling pumping stolon, and top seedling starts to take root to carry out arranging and fixes,
Seedling grows the independent seedling of formation in hole tray.
As currently preferred technical scheme, described detoxification original seed Seedling acquisition methods is: April, clip was new from plant
Longer stolon stem apex rinses 1.5-2h through flowing water, on the clean workbench of aseptic behaviour, first with 75% medical alcohol sterilization
30s, the most again with 0.1% mercuric chloride water sterilization 8min, peels off outer layer bag sheet after last aseptic water washing 3-4 time, is inoculated by stem apex
Culture medium one carries out inducing initial culture, within 25 ± 2 days, obtains aseptic seedling；Above-mentioned aseptic seedling subculture is enterprising in culture medium one
Row subculture multiplication expanding propagation, carries out root culture by the switching breeding independent seedling in culture medium two in 25 ± 2 days；Aforesaid operations be
25 ± 2 DEG C, intensity of illumination about 2400 ± 200lx, light application time every day 14～16h culturing room in carry out, culture medium one refers to
MS+0.5mg/L 6-BA+0.1mg/L NAA+30g/L sucrose+6.2g/L agar, the culture medium of pH=5.8, it is used for inducing just generation
And subculture multiplication；Culture medium two refers to 1/2MS+30g/L sucrose+6.2g/L agar, the culture medium of pH=5.8, is used for taking root；25
Within ± 2 days, will sprout the detoxification original seed Seedling removal group training room of complete root system, the sealed membrane opening tissue cultured seedling of taking root in indoor is tamed and dociled
Change, place 1～2 day under normal temperature condition, root culture medium is cleaned up, be transplanted to volume proportion peat: perlite: Vermiculitum is
3: 2: 1, in substrate, moisturizing promotees to live, and within about 18 ± 2 days, obtains detoxification original seed Seedling.
As currently preferred technical scheme, the hole tray edge of described filling substrate is slightly below surrounding soil, and insulation is protected
Water；Booth possesses insulation, takes shelter from rain, insect protected, ventilation function, substrate high-temperature sterilization.
As currently preferred technical scheme, described suitable management mode is: after potted plant field planting two weeks, is spaced 10
Spraying 2 ‰ water-soluble phosphoric acid potassium dihydrogens about it and nitrogenous fertilizer mixed liquor promotes pumping, once, substrate keeps half to the most each micro-water spray
Moistening, this schema management is to mid-October；Mercury dropped after November, closes canopy insulation and is not less than 12 DEG C, open canopy daytime and lead to night
Wind keeps 25-30 DEG C, and prevention plant pumping is slow, is spaced and within about 15 days, sprays 2 ‰ water-soluble phosphoric acid potassium dihydrogens and nitrogenous fertilizer mixing
Liquid, this schema management is to seedling outbound mid-March.
As currently preferred technical scheme, described original seed Seedling changes basin bore 20cm used, basin deep 20cm during pot transplanting.
Present invention process flow process simply, easily grasp, strong operability, have with state of the art knowledge and conventional means
Have the following advantages and effect:
The present invention obtains detoxification original seed Seedling by step (1) (2), isolates nursery, and sterilization is bred mediated matrix, effectively stopped
The source of pest and disease damage and infecting.
The present invention is taken root in step (3) (4) detoxification original seed seedling proliferation, after late July transplants, does not use growth regulator,
Ensure that maternal nutrition growth and variety, prolongation is bred time promotion increase and is bred coefficient again.
Method provided by the present invention is used for Fructus Fragariae Ananssae virus-elimination seedlings mating system, and it is raw that the method not only breaks through Routine Test Lab
Thing raising technology is difficult to the difficult problem popularized, and the kind sexual involution and the cross-contamination that cause after also preventing Routine Test Lab repeatedly subculture are drawn
Send out the security risk of hereditary variation, reduce heavy subculture work simultaneously, reduce labour force, reduce material cost.
Method provided by the present invention is used for Fructus Fragariae Ananssae virus-elimination seedlings mating system, Appropriate application booth insect protected, takes shelter from rain and protects
The function of temperature promotes parent growth and stolon pumping, avoids again open country to breed the risk that pest and disease damage brings.
Method provided by the present invention is used for Fructus Fragariae Ananssae virus-elimination seedlings mating system, and the method both obtained to outbound mid-March
Virus-elimination seedlings quantity, breeds coefficient and reaches 42～53, strawberry seedling resident time before and after linking produces upper Clear and Bright very well again.
Detailed description of the invention
Embodiment 1
(1) acquisition of detoxification original seed Seedling: the stolon stem apex flowing water punching that April 20 in 2015, clip from plant newly grew
Washing, sterilize, inoculate, May 14 obtained aseptic seedling；Take above-mentioned aseptic seedling subculture multiplication, rooting and transplant promotees to live, and July 23 obtained
Original seed Seedling；
(2) nursery prepares: 1) select, away from Fructus Fragariae Ananssae, the paddy field of vegetable solanaceous vegetables, ground grading, to be filled also by 32 hole dishes
Tiling earth's surface after compacting Fructus Fragariae Ananssae substrate, makes hole tray edge be slightly below surrounding soil, and irrigation and drainage are convenient, reserved detoxification original seed Seedling and management
Passage；2) building booth on nursery, canopy film uses four pieces of polyethylene films, and top is two pieces of crimping, and another two pieces is both sides skirt films,
Opening ventilation, Guan Bi is taken shelter from rain, and surrounding installs fly net and automatic micro-sprinkling irrigation facility；3) June 26, substrate is watered foot water and boil in a covered pot over a slow fire canopy
High-temperature sterilization, July 17 opened aeration-cooling；
(3) change pot transplanting: detoxification original seed Seedling is put in booth by July 27, transplant after 1 week of centralized management, use basin
Cultivation mode, basin bore 20cm, the deep 20cm of basin, basin mouth edge is concordant with hole tray, and seedling, conventional pipe are bred in the most reserved hole tray 48 hole
Reason rich water, booth is opened ventilation, and is added a cover sunshade net protection to early September；
(4) detoxification original seed Seedling pumping is promoted: after field planting two weeks, be spaced and within about 10 days, spray 2 ‰ water-soluble phosphorus acid dihydrides
Potassium and nitrogenous fertilizer mixed liquor promote pumping, and once, substrate keeps semi-moist to the most each micro-water spray, and this schema management is to mid-October；
Mercury dropped after November, closes canopy insulation and is not less than 12 DEG C night, opens canopy ventilation daytime and keeps 25-30 DEG C, prevent plant pumping
Slowly, being spaced and within about 15 days, spray 2 ‰ water-soluble phosphoric acid potassium dihydrogens and nitrogenous fertilizer mixed liquor, this schema management is to mid-March；
(5) seedling arranges fixing: after detoxification original seed Seedling pumping stolon, and top seedling starts to take root to carry out arranging and fixes,
Seedling grows the independent seedling of formation in hole tray.
That adds up seedling breeds coefficient: the coefficient of averagely breeding of tested variety is: red cheek 36.68, Zijin the reddest 53.16.
Cultivar origin is: red cheek is the Japan cultivars introduced in 1999, and Zijin is the reddest is 2015 to be identified by Jiangsu Province, is Jiangsu Province's agriculture
The new varieties of the autonomous selection-breeding of industry academy of science.
Claims (5)
1. a simple Fructus Fragariae Ananssae virus-elimination seedlings mating system, its step includes:
(1) acquisition of detoxification original seed Seedling: the stolon stem apex that April, clip from plant newly grew, flowing water rinse, sterilization,
Inoculation, growth obtains aseptic seedling；Take above-mentioned aseptic seedling subculture multiplication, rooting and transplant promotees to live, and growth obtains detoxification original seed Seedling；
(2) nursery prepares:
2.1) addressing, whole: the address challenges of selection is filled also away from Fructus Fragariae Ananssae, the paddy field of vegetable solanaceous vegetables, ground grading, hole tray
Tiling earth's surface after compacting substrate, makes hole tray edge be slightly below surrounding soil, and irrigation and drainage are convenient, by reserved detoxification original seed Seedling position and pipe
Reason passage；
2.2) booth is built: on nursery, build booth, it is desirable to winter can be incubated, insect protected of taking shelter from rain summer, ventilation and penetrating light, illumination
Time strong, sunshade net protection can be added a cover, automatic micro-sprinkling irrigation facility is installed simultaneously, can micro-spray repair water, can precision fertilizing；
2.3) substrate is watered before field planting foot water, and to boil in a covered pot over a slow fire canopy high-temperature sterilization aeration-cooling to field planting front opening；
(3) pot transplanting field planting is changed: late July, by seedling exercising in the original seed rearmounted booth of Seedling detoxification, transplants after 1 week of centralized management,
Detoxification original seed Seedling uses potted plant mode, and basin mouth edge is concordant with hole tray, around reserves hole tray and breeds seedling, Routine Management rich water,
Booth requires ventilation and penetrating light, when illumination is strong, adds a cover sunshade net protection；
(4) detoxification original seed Seedling stolon pumping is promoted: use suitable management mode to promote detoxification original seed Seedling stolon pumping；
(5) seedling arranges fixing: after detoxification original seed Seedling pumping stolon, and top seedling starts to take root to carry out arranging and fixes, seedling
In hole tray, growth forms independent seedling.
Simple Fructus Fragariae Ananssae virus-elimination seedlings mating system the most according to claim 1, it is characterised in that: described detoxification original seed Seedling obtains
Access method is: the stolon stem apex that April, clip from plant newly grew rinses 1.5-2h through flowing water, only works aseptic behaviour
On platform, first with 75% medical alcohol sterilization 30s, the most again with 0.1% mercuric chloride water sterilization 8min, last aseptic water washing 3-4 time
After peel off outer layer bag sheet, stem apex is seeded in culture medium one carry out induce initial culture, 25 ± 2 days obtain aseptic seedling；By upper
State aseptic seedling subculture in culture medium one, carry out subculture multiplication expanding propagation, within 25 ± 2 days, the switching of independent seedling will be bred in culture medium
Two carry out root culture；Aforesaid operations be 25 ± 2 DEG C, intensity of illumination about 2400 ± 200lx, light application time every day 14～16h
Culturing room in carry out, culture medium one refers to MS+0.5mg/L 6-BA+0.1mg/L NAA+30g/L sucrose+6.2g/L agar,
The culture medium of pH=5.8, is used for inducing just generation and subculture multiplication；Culture medium two refers to 1/2MS+30g/L sucrose+6.2g/L fine jade
Fat, the culture medium of pH=5.8, it is used for taking root；Within 25 ± 2 days, the detoxification original seed Seedling removal group training room of complete root system will be sprouted, in room
The sealed membrane inside opening tissue cultured seedling of taking root is tamed, and places 1～2 day, root culture medium cleaned up under normal temperature condition, moves
Planting to volume proportion peat: perlite: Vermiculitum is 3: 2: 1, in substrate, moisturizing promotees to live, and within 18 ± 2 days, obtains detoxification original seed Seedling.
Simple Fructus Fragariae Ananssae virus-elimination seedlings mating system the most according to claim 2, it is characterised in that: the cave of described filling substrate
Plate edge is slightly below surrounding soil, is incubated water conservation；Booth possesses insulation, takes shelter from rain, insect protected, ventilation function, and substrate is through high-temperature sterilization.
Simple Fructus Fragariae Ananssae virus-elimination seedlings mating system the most according to claim 3, it is characterised in that: described suitable management mould
Formula is: after potted plant field planting two weeks, is spaced and within about 10 days, sprays 2 ‰ water-soluble phosphoric acid potassium dihydrogens and nitrogenous fertilizer mixed liquor and promote to take out
Raw, once, substrate keeps semi-moist to the most each micro-water spray, and this schema management is to mid-October；Mercury dropped after November, closes canopy
Insulation is not less than 12 DEG C night, opens canopy ventilation daytime and keeps 25-30 DEG C, and prevention plant pumping is slow, is spaced and sprays for about 15 days
2 ‰ water-soluble phosphoric acid potassium dihydrogens and nitrogenous fertilizer mixed liquor, this schema management is to seedling outbound mid-March.
Simple Fructus Fragariae Ananssae virus-elimination seedlings mating system the most according to claim 4, it is characterised in that: described original seed Seedling changes basin and moves
Basin bore 20cm used, basin deep 20cm during cultivation.
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| CN201610659584.2A CN106258370A (en) | 2016-08-11 | 2016-08-11 | Simple Fructus Fragariae Ananssae virus-elimination seedlings mating system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112167061A (en) * | 2020-10-21 | 2021-01-05 | 安顺欣丞农业科技发展有限公司 | Propagation method for cultivating plateau strawberry virus-free seedlings |
| CN115715523A (en) * | 2022-11-29 | 2023-02-28 | 云南省农业科学院花卉研究所 | Method for rapidly constructing and propagating virus-free seed source of strawberries in Yunnan province |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112167061A (en) * | 2020-10-21 | 2021-01-05 | 安顺欣丞农业科技发展有限公司 | Propagation method for cultivating plateau strawberry virus-free seedlings |
| CN115715523A (en) * | 2022-11-29 | 2023-02-28 | 云南省农业科学院花卉研究所 | Method for rapidly constructing and propagating virus-free seed source of strawberries in Yunnan province |
| CN115715523B (en) * | 2022-11-29 | 2023-07-28 | 云南省农业科学院花卉研究所 | Rapid construction and propagation method for strawberry virus-free seed source in Yunnan middle region |
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