CN102598990B - Method for increasing transplanting survival rate of tissue culture seedlings of ginseng - Google Patents
Method for increasing transplanting survival rate of tissue culture seedlings of ginseng Download PDFInfo
- Publication number
- CN102598990B CN102598990B CN 201210087256 CN201210087256A CN102598990B CN 102598990 B CN102598990 B CN 102598990B CN 201210087256 CN201210087256 CN 201210087256 CN 201210087256 A CN201210087256 A CN 201210087256A CN 102598990 B CN102598990 B CN 102598990B
- Authority
- CN
- China
- Prior art keywords
- matrix
- film
- survival rate
- seedling
- genseng
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for increasing transplanting survival rate of tissue culture seedlings of ginseng, which mainly includes medium preparation, mulching and seedling exercising and transplanting. The medium prepared is suitable for rooting and growth of the tissue culture seedlings of ginseng and contains humus soil under woods and black soil in northeast China. In the simulated wild environment, beneficial bacteria and organic matters in the humus soil guarantee nutrition feed for the tissue culture seedlings. The transplanting survival rate of the tissue culture seedlings cultured by the steps is up to 99%.
Description
Technical field
The present invention relates to a kind of method that improves genseng tissue cultivating seedling transplanting survival rate.
Background technology
Genseng is the traditional rare traditional Chinese medicine of China, and its active ingredient is ginsenoside, has effects such as tonifying Qi, beneficial lung, strong, nourishing, antifatigue.Along with the development of society, the genseng market demand increases day by day.At present, can artificially regulate the imbalance between supply and demand that genseng growth of cell culture amount and saponin(e output are alleviated genseng by cell engineering.The wild ginseng self-sow is in the virgin forest of original gloomy mountain, and what have reaches a century, through cultivation, there is not the composition of chemical fertilizer, it is very arduous to gather, and medical value is high, is a kind of rare traditional Chinese medicine, also be the high green rare generation treasure of value for preservation, very worthy.All the more minimizing in excavation for many years, the disappearance of most of northeastward area, at present in the Changbai mountain, Jilin national natural reserves or mountain area on every side exist.
Research for the genseng proliferation at present mainly concentrates on seminal propagation and tissue culture propagation, however seminal propagation aberration rate height, and the time is very long, and by tissue culture propagation, though weak point consuming time, the reproductive efficiency height, yet, had a strong impact on its marketing and used because transplanting survival rate can not get assurance.The present invention particularly simulates the environment of its self-sow by changing the transplanting environment of tissue cultivating seedling, has improved the transplanting survival rate of genseng greatly.
Summary of the invention
The technical problem that the present invention mainly solves provides a kind of method that improves genseng tissue cultivating seedling transplanting survival rate.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of method that improves genseng tissue cultivating seedling transplanting survival rate comprises:
(1) preparation matrix: the composition by following weight portion is formed: high-quality leaf mould 50-70 part, loam 30-50 part, vermiculite 3-5 part, the rice chaff 10-15 part that fully ferments, tpn 0.3-0.6 part, spray matrix, every square metre of soil formalin 45m1 with behind 40～80 times of the formalin dilute with waters;
(2) above-mentioned treated matrix shop is sprinkled upon in the furrow of putting in order, the thick 50-80cm of matrix, film covered 5 days then, and airing can be transplanted the genseng tissue cultivating seedling in 3 days again;
Described film covers the one deck of the covering earlier white plastic film that is meant at soil surface, cover the second layer black plastic film that is used for sealing the ground floor film then on the ground floor film, the distance between wherein said ground floor film and the second layer film is 30-40 centimetre.
(3) acclimatization and transplants: earlier blake bottle is moved to the greenhouse, natural conditions lower refining seedling 4d unties then and seals film, hardening 6d, suitably shading during the hardening, making intensity of illumination is 35% of natural daylight, takes out seedling after hardening is finished and cleans the medium that root adheres to, and is transplanted in the described matrix through sterilizing of step (2), temperature is controlled at 22-24 ℃, preceding 7d humidity is controlled at about 70%, reduces humidity later on gradually, until transplanted seedling is placed under the natural conditions.
The described matrix of step (1) preferably is made up of the composition of following weight portion: 60 parts of sylvan life leaf moulds, 40 parts in northeast black earth, 4 parts of vermiculites, 13 parts of rice chaffs that fully ferment, 0.4 part of tpn.
The invention has the beneficial effects as follows:
(1) utilizes method of the present invention, owing to one deck black plastic film is set at skin, airtight for a long time by black plastic film, solar radiation heat energy is constantly accumulated wherein, make temperature rising in the soil, keep higher inhibition for a long time or kill temperature, effectively suppress or kill multiple germ in the soil, pest such as insect and weeds, so can allow the temperature of soil be up to 80 ℃ within a certain period of time, under black plastic film, also have simultaneously one deck white film, white film the high humility that can keep soil is set, this hot and humid down, at first, fertilizer is fermentation maturity fully, in addition, can effectively suppress or kill the damage by disease and insect in the soil, and can not cause environmental pollution, save production cost;
(2) institute of the present invention matrix prepared is fit to taking root and grow of genseng tissue cultivating seedling, and also has sylvan life leaf mould and northeast black earth in the matrix, in this simulation wild environment, and the nutrition supply that abundant beneficial bacterium and organic matter have guaranteed tissue cultivating seedling in the leaf mould.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby protection scope of the present invention is made more explicit defining so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that.
Embodiment one
A kind of method that improves genseng tissue cultivating seedling transplanting survival rate is characterized in that, comprising:
(1) preparation matrix: the composition by following weight portion is formed: 50 parts of high-quality leaf moulds, 30 parts in loam, 3 parts of vermiculites, 10 parts of rice chaffs that fully ferment, 0.3 part of tpn, spray matrix, every square metre of soil formalin 45m1 with behind 40 times of the formalin dilute with waters;
(2) above-mentioned treated matrix shop is sprinkled upon in the furrow of putting in order, the thick 50cm of matrix, film covered 5 days then, and airing can be transplanted the genseng tissue cultivating seedling in 3 days again;
Described film covers the one deck of the covering earlier white plastic film that is meant at soil surface, cover the second layer black plastic film that is used for sealing the ground floor film then on the ground floor film, the distance between wherein said ground floor film and the second layer film is 30-40 centimetre.
(3) acclimatization and transplants: earlier blake bottle is moved to the greenhouse, natural conditions lower refining seedling 4d unties then and seals film, hardening 6d, suitably shading during the hardening, making intensity of illumination is 35% of natural daylight, takes out seedling after hardening is finished and cleans the medium that root adheres to, and is transplanted in the described matrix through sterilizing of step (2), temperature is controlled at 22-24 ℃, preceding 7d humidity is controlled at about 70%, reduces humidity later on gradually, until transplanted seedling is placed under the natural conditions.
Embodiment two
A kind of method that improves genseng tissue cultivating seedling transplanting survival rate comprises:
(1) preparation matrix: the composition by following weight portion is formed: 70 parts of high-quality leaf moulds, 50 parts in loam, 5 parts of vermiculites, 15 parts of rice chaffs that fully ferment, 0.6 part of tpn, spray matrix, every square metre of soil formalin 45m1 with behind 80 times of the formalin dilute with waters;
(2) above-mentioned treated matrix shop is sprinkled upon in the furrow of putting in order, the thick 80cm of matrix, film covered 5 days then, and airing can be transplanted the genseng tissue cultivating seedling in 3 days again;
Described film covers the one deck of the covering earlier white plastic film that is meant at soil surface, cover the second layer black plastic film that is used for sealing the ground floor film then on the ground floor film, the distance between wherein said ground floor film and the second layer film is 30-40 centimetre.
(3) acclimatization and transplants: earlier blake bottle is moved to the greenhouse, natural conditions lower refining seedling 4d unties then and seals film, hardening 6d, suitably shading during the hardening, making intensity of illumination is 35% of natural daylight, takes out seedling after hardening is finished and cleans the medium that root adheres to, and is transplanted in the described matrix through sterilizing of step (2), temperature is controlled at 22-24 ℃, preceding 7d humidity is controlled at about 70%, reduces humidity later on gradually, until transplanted seedling is placed under the natural conditions.
Embodiment three
A kind of method that improves genseng tissue cultivating seedling transplanting survival rate comprises:
(1) preparation matrix: the composition by following weight portion is formed: 60 parts of sylvan life leaf moulds, 40 parts in northeast black earth, 4 parts of vermiculites, 13 parts of rice chaffs that fully ferment, 0.4 part of tpn, spray matrix, every square metre of soil formalin 45m1 with behind 60 times of the formalin dilute with waters;
(2) above-mentioned treated matrix shop is sprinkled upon in the furrow of putting in order, the thick 60cm of matrix, film covered 5 days then, and airing can be transplanted the genseng tissue cultivating seedling in 3 days again;
Described film covers the one deck of the covering earlier white plastic film that is meant at soil surface, cover the second layer black plastic film that is used for sealing the ground floor film then on the ground floor film, the distance between wherein said ground floor film and the second layer film is 30-40 centimetre.
(3) acclimatization and transplants: earlier blake bottle is moved to the greenhouse, natural conditions lower refining seedling 4d unties then and seals film, hardening 6d, suitably shading during the hardening, making intensity of illumination is 35% of natural daylight, takes out seedling after hardening is finished and cleans the medium that root adheres to, and is transplanted in the described matrix through sterilizing of step (2), temperature is controlled at 22-24 ℃, preceding 7d humidity is controlled at about 70%, reduces humidity later on gradually, until transplanted seedling is placed under the natural conditions.
Among above-mentioned three embodiment, the transplanting survival rate of genseng tissue cultivating seedling has reached 96%, 98%, 99% respectively.
The above only is embodiments of the invention; be not so limit claim of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.
Claims (2)
1. a method that improves genseng tissue cultivating seedling transplanting survival rate is characterized in that, comprising:
(1) preparation matrix: the composition by following weight portion is formed: high-quality leaf mould 50-70 part, loam 30-50 part, vermiculite 3-5 part, the rice chaff 10-15 part that fully ferments, tpn 0.3-0.6 part, to spray matrix behind 40～80 times of the formalin dilute with waters to the matrix disinfection, every square metre of matrix soil formalin 45m1;
(2) above-mentioned treated matrix shop is sprinkled upon in the furrow of putting in order, the thick 50-80cm of matrix, film covered 5 days then, and airing can be transplanted the genseng tissue cultivating seedling in 3 days again;
Described film covers the surface that is meant at matrix soil and covers one deck white plastic film earlier, cover the second layer black plastic film that is used for sealing the ground floor film then on the ground floor film, the distance between wherein said ground floor film and the second layer film is 30-40 centimetre.
(3) acclimatization and transplants: earlier blake bottle is moved to the greenhouse, natural conditions lower refining seedling 4d unties then and seals film, hardening 6d, suitably shading during the hardening, making intensity of illumination is 35% of natural daylight, takes out seedling after hardening is finished and cleans the medium that root adheres to, and is transplanted in the described matrix through sterilizing of step (2), temperature is controlled at 22-24 ℃, preceding 7d humidity is controlled at 70%, reduces humidity later on gradually, until transplanted seedling is placed under the natural conditions.
2. the method for raising genseng tissue cultivating seedling transplanting survival rate according to claim 1, it is characterized in that the described matrix of step (1) is made up of the composition of following weight portion: 60 parts of sylvan life leaf moulds, 40 parts in northeast black earth, 4 parts of vermiculites, 13 parts of rice chaffs that fully ferment, 0.4 part of tpn.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210087256 CN102598990B (en) | 2012-03-29 | 2012-03-29 | Method for increasing transplanting survival rate of tissue culture seedlings of ginseng |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210087256 CN102598990B (en) | 2012-03-29 | 2012-03-29 | Method for increasing transplanting survival rate of tissue culture seedlings of ginseng |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102598990A CN102598990A (en) | 2012-07-25 |
| CN102598990B true CN102598990B (en) | 2013-07-24 |
Family
ID=46517074
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210087256 Active CN102598990B (en) | 2012-03-29 | 2012-03-29 | Method for increasing transplanting survival rate of tissue culture seedlings of ginseng |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102598990B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108605799A (en) * | 2016-11-30 | 2018-10-02 | 丹阳市大丰收苗木专业合作社 | A kind of ginkgo tissue-cultured seedling transplanting medium |
| CN108260488B (en) * | 2018-01-29 | 2020-05-15 | 吉林紫鑫红石种养殖有限公司 | Technical method for high-yield under-forest ginseng tending |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1774988A (en) * | 2005-11-23 | 2006-05-24 | 魏本胜 | Special-shaped handicraft ginseng cultivating and processing method |
| CN101563983A (en) * | 2009-06-06 | 2009-10-28 | 刘文龙 | Artificial cultivation technology for cultivating panicled fameflower |
-
2012
- 2012-03-29 CN CN 201210087256 patent/CN102598990B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1774988A (en) * | 2005-11-23 | 2006-05-24 | 魏本胜 | Special-shaped handicraft ginseng cultivating and processing method |
| CN101563983A (en) * | 2009-06-06 | 2009-10-28 | 刘文龙 | Artificial cultivation technology for cultivating panicled fameflower |
Non-Patent Citations (5)
| Title |
|---|
| 农田地人参栽培技术要点;崔东河等;《人参研究》;20101231;第28-29页 * |
| 崔东河等.农田地人参栽培技术要点.《人参研究》.2010,第28-29页. |
| 崔德深.美国人参及其栽培技术概况.《特产研究》.1982,第14-17页. |
| 美国人参及其栽培技术概况;崔德深;《特产研究》;19820331;第14-17页 * |
| 钟国农.人参栽培技术(一).《农村实用技术》.2006,第26-28页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102598990A (en) | 2012-07-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103947460B (en) | A kind of field production method of Stropharia rugoso-annulata | |
| CN102523881B (en) | Industrial cultivation method for dendrobium candidum | |
| CN105993590A (en) | Culturing method for sporocarp of Morchella | |
| CN103988649B (en) | A kind of large leaf tea tree rapid seedling cultivation method | |
| CN102919107B (en) | Propagating method of orchids in Xinjiang region | |
| CN103880521B (en) | A kind of method of needle mushroom dreg culture base-material and cultivating bisporous mushroom thereof | |
| CN102715090B (en) | Subprostrate sophora root tissue culture seedling industrialization production method | |
| CN105766385B (en) | A kind of plant protection method improving virus-free basic potato seed yield | |
| CN102763599B (en) | Podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method | |
| CN102119655A (en) | Natural light rapid breeding method for dendrobium officinale | |
| CN103039262A (en) | Ginseng cultivation method with nutrient blocks in greenhouse | |
| CN105660181A (en) | Method for cultivating nostoc commune outdoors | |
| CN104429951A (en) | Pollution-free large-scale cultivation method for wild dendrobium officinale | |
| CN102960226A (en) | Dendrobium officinale Kimura et Migo tissue culture seedling acclimatization method | |
| CN103988743A (en) | Banana planting method | |
| CN108925363A (en) | A kind of method of the imitative wild cultivating ganoderma of hayashishita | |
| CN106941928A (en) | A kind of implantation methods of Phellinus | |
| CN102598990B (en) | Method for increasing transplanting survival rate of tissue culture seedlings of ginseng | |
| CN105103943A (en) | Bionics wild cultivation method for lepista glaucocana | |
| CN112088717A (en) | Method for cultivating cordyceps sinensis in field | |
| CN106717819A (en) | A kind of implantation methods of dendrobium candidum | |
| CN104303966B (en) | A kind of cultural method of wild gold lotus | |
| CN103621291A (en) | Low tunnel reflective film white celery growing method | |
| CN106172002A (en) | A kind of production method of Folium Artemisiae Argyi tissue cultured seedling | |
| CN106258370A (en) | Simple Fructus Fragariae Ananssae virus-elimination seedlings mating system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |