CN103880521B - A kind of method of needle mushroom dreg culture base-material and cultivating bisporous mushroom thereof - Google Patents

A kind of method of needle mushroom dreg culture base-material and cultivating bisporous mushroom thereof Download PDF

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CN103880521B
CN103880521B CN201410058391.2A CN201410058391A CN103880521B CN 103880521 B CN103880521 B CN 103880521B CN 201410058391 A CN201410058391 A CN 201410058391A CN 103880521 B CN103880521 B CN 103880521B
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mushroom
culture base
needle
needle mushroom
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葛静波
陈春化
王浩东
沈业庭
季林章
孙晓亮
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SIYANG COUNTY INSTITUTE OF AGRICULTURAL SCIENCES
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Abstract

The invention discloses a kind of method of needle mushroom dreg culture base-material and cultivating bisporous mushroom thereof, by needle mushroom dreg 30-60 part, cow dung 35 parts, bagasse 0.5-1 part, silkworm excrement 0.5-1.5 part, composite fertilizer 1 part, lime 1-1.5 part, gypsum 0.5-1 part, calcium superphosphate 2 parts, the Twospore Mushroom fruiting phase of producing by building heap, fermentation, sowing, earthing, fruiting and series of steps of gathering shifts to an earlier date 7-10 days, adopts the mushroom phase to extend 10-15 days, needle mushroom dreg is recycled, decreases the pollution of bacterium slag to environment.

Description

A kind of method of needle mushroom dreg culture base-material and cultivating bisporous mushroom thereof
Technical field
The present invention relates to technical field of edible fungi production, particularly relate to a kind of method of needle mushroom dreg culture base-material and cultivating bisporous mushroom thereof.
Background technology
Needle mushroom formal name used at school hair handle money bacterium, also known as the little fiery mushroom of hair handle, structure bacterium, plain mushroom, dried mushroom, plain wild rice, freezes bacterium, golden mushroom, intelligence mushroom etc.; Because its stem is elongated, like Flos Hemerocallis, therefore claim pin gold mushroom, belonging to Agaricales Bai Mo section pin gold mushroom and belong to, is a kind of bacterium algae lichens.Needle mushroom has very high medicinal dietary function; Needle mushroom is not containing chlorophyll, not there is photosynthesis, carbohydrate can not be manufactured, but can grow in dark surrounds completely, must absorb ready-made organic substance from substratum, as the degradation product of carbohydrate, protein and fat, be saprophytic nutrition type, be a kind of heterotroph, belong to club fungi.Needle mushroom is a kind of timber saprophytic microorganism, and easily growth is on the withered trunk and stub of the deciduous trees such as willow, elm, poplar tree.
Accelerating development circular agriculture as propelling Construction of Modern Agriculture, is the Industry support of strengthening building Socialist New.And be that the circular agriculture pattern of tie effectively utilizes agricultural resource to be particularly a kind of best mode to the efficient disposal and utilization of agricultural crop straw and feces of livestock and poultry with planting edible mushroom.Along with the development of factory edible fungi industry, produce a large amount of bacterium slag also containing rich in protein and other nutritive ingredients after producing, agriculture production has higher utility value.But China is also very low to the utilization ratio of bacterium slag at present.Found by the continuous exploratory development of nearly 3 years, utilize edible fungi residue to can be used as cultivation of agaricus bisporus raw material feasible, not only there is joint production cost, shorten the fruiting phase, the object of increasing economic efficiency, meanwhile, for the efficient circulation utilization of bacterium slag walks out a new way, along with the development of social city, technicalization, hommization, design is a kind of cost-saving, the method utilizing needle mushroom dreg culture base-material and cultivating bisporous mushroom thereof of output increased, to meet the need of market, is very important.
Along with the production-scale continuous expansion of factory edible fungi, produce a large amount of bacterium slag, cause the huge waste of agriculture organic resource, what have also causes new environmental pollution.Therefore, how bacterium slag is handled in environmental protection effectively well, has become a more and more severeer problem.Containing a large amount of nutritive ingredients such as tropina, amino acid, Mierocrystalline cellulose, hemicellulose and nitrogen, phosphorus, potassium in needle mushroom dreg, detect according to the bacterium slag of Agricultural development quality safety verification center, academy of agricultural sciences of Jiangsu Province to needle mushroom factorial praluction enterprise, containing the crude fat of 2.25%, robust fibre, the total reducing sugar of 1.62%, nitrogen, the phosphorus of 0.61%, the potassium of 0.84% of 0.27% of 31.23% in bacterium slag, nutritious, be the ideal material of alternative straw.
Summary of the invention
the technical problem solved:
The application, for cycle of mycelium growing period, spontaneous fermentation in the method for existing cultivating bisporous mushroom and the technical problem such as the Secondary Fermentation cycle is long, with high costs, provides a kind of method utilizing needle mushroom dreg culture base-material and cultivating bisporous mushroom thereof.
technical scheme:
A kind of needle mushroom dreg culture base-material, the raw materials by weight portion proportioning of needle mushroom dreg culture base-material is as follows: needle mushroom dreg 30-60 part, cow dung 35 parts, bagasse 0.5-1 part, silkworm excrement 0.5-1.5 part, composite fertilizer 1 part, lime 1-1.5 part, gypsum 0.5-1 part, calcium superphosphate 2 parts.
As a preferred technical solution of the present invention: the method steps of the cultivating bisporous mushroom of described needle mushroom dreg culture base-material is:
The first step: needle mushroom dreg is pulverized, dries rear for subsequent use;
Second step: prewet cow dung 2-3 days, Hou Jiandui is mixed with needle mushroom dreg, the turning when piling temperature and declining by 70-75 DEG C, composite fertilizer and bagasse add when building heap and first time turning, calcium superphosphate and gypsum add when second time turning, and lime and silkworm excrement add when third time turning, regulate pH at 7.8-8 after the 4th turning, water content 65%-67%, obtained needle mushroom dreg culture base-material;
3rd step: the needle mushroom dreg culture base-material that heap makes is transported in the mushroom room after sterilization and is also evenly put on mushroom bed, close mushroom room door window, mushroom room temperature is maintained at 62-65 DEG C by ventilating pit, 50-55 DEG C is cooled to after 8-10 hour, maintain and be cooled to 45-50 DEG C after 3-4 days, keep after 12 hours, opening door and window cooling;
4th step: pick the foreign material in needle mushroom dreg culture base-material, leveling charge level, makes bed thickness be 20-25cm, opens door and window and ammonia in mushroom room is left, and maintains mushroom room temperature at 22-28 DEG C;
5th step: first 2/3 bacterial classification is evenly sprinkling upon on needle mushroom dreg substratum charge level, after being covered with the needle mushroom dreg culture base-material of 0.5-1cm, remaining bacterial classification is sprinkled, with liming, sterilized in mushroom room, close the doors and windows 3-5 days, keep mushroom room temperature at 22-26 DEG C, relative air humidity remains on 75-85%;
After 6th step: 15-20 days, be watered with SD-1750 or derosal, sprinkling prevention is carried out to needle mushroom dreg culture base-material and kills disease and pest in material, 1% lime powder will be admixed in casingmaterial, adjustment casingmaterial potential of hydrogen is 7.5-8, water content reaches earthing after 40-45%, the thick 3-3.5cm of soil layer, keep mushroom room temperature at 22-26 DEG C, relative air humidity 85-90%, cool to 15-20 DEG C when mycelia grows to soil layer 2/3, spray knot mushroom water, kept atmospheric moisture at 85%-95% after continuous spraying 2-3 days;
7th step: control room temperature at 15-18 DEG C, every day ventilates 1-2 time, each 1-2 hour, can gather when cap grows to when 3-4cm, mycoderm not yet break.
As a preferred technical solution of the present invention: the quantity of described 5th step bacterial classification is every square metre of culture base-material 500ml/ bottle bacterial classification 1.5-2 bottle.
As a preferred technical solution of the present invention: will carry out disinfection to casingmaterial before described 6th step earthing, spray clear water simultaneously and keep casingmaterial water content 40%.
As a preferred technical solution of the present invention: after adopting mushroom in described 7th step lower batch mushroom grow to that soya bean is large, temperature 18 DEG C time, every square metre of culture base-material 0.5 kg of phosphoric acid potassium dihydrogen solution sprays, and potassium dihydrogen phosphate mass percentage concentration is 5 ‰.
beneficial effect:
Compared to the prior art the method for a kind of needle mushroom dreg culture base-material of the present invention and cultivating bisporous mushroom thereof adopts above technical scheme, there is following technique effect: 1, every 5000 square chi cost-saved 4540 yuan, standard mushroom room, building, the fruiting phase shifts to an earlier date 7-10 days, adopt mushroom phase prolongation 10-15 days, increase yield 200 kilograms, the output value 1400 yuan, accumulate benefit 5940 yuan; 2, needle mushroom dreg is recycled, decrease the pollution of bacterium slag to environment.
Embodiment
In following examples, bacterial classification selects the agaricus bisporus hybrid kind As2796 of Twospore Mushroom bacterial classification institute of Fujian Province seed selection, and all composite fertilizers are the ammonium potassium phosphate composite fertilizer that Stanley Fertilizer Co., Ltd produces.
embodiment 1:
Proportioning takes needle mushroom dreg 60 parts by weight, cow dung 35 parts, bagasse 0.5 part, silkworm excrement 1 part, composite fertilizer 1 part, 1 part, lime, 1 part, gypsum, calcium superphosphate 2 parts, cow dung is prewetted 3 days, Hou Jiandui is mixed with needle mushroom dreg, the turning when piling temperature and declining by 75 DEG C, add when composite fertilizer and bagasse first time turning, calcium superphosphate and gypsum add when second time turning, lime and silkworm excrement add when third time turning, after 4th turning, culture base-material is light coffee color, there is material fragrance, slightly ammonia odor, regulate pH 7.9, water content 66%, heap shape is conical.
Also evenly be put on mushroom bed in mushroom room after the culture base-material that heap makes was transported sterilization in one day, close mushroom room door window, mushroom room temperature is maintained at 64 DEG C, kill harmful microorganism and the larva remained in culture material, worm's ovum, pathogenic bacteria and miscellaneous bacteria after 9 hours by ventilating pit; Then top and the bottom ventilation hole is opened, be cooled to 50 DEG C, maintain 4 days, promote that the beneficial microorganisms such as high temperatures actinomycetes are fully bred and Nutrient Transformation, 45 DEG C are cooled to after forming the tropina and various VITAMIN and amino acid easily being absorbed by Twospore Mushroom mycelia in a large number and utilize, keep after 12 hours, opening door and window cooling, a large amount of Thermoactinomyces albus is throughout the whole substratum bed of material, culture base-material is dark brown, without ammonia stink, there is material fragrance, culture base-material is soft, good permeability, water content 63%.
Every square metre of culture base-material 500ml/ bottle bacterial classification 2 bottles, prepares enough bacterial classification.Pick the foreign material in culture base-material, leveling charge level, makes bed thickness be 25cm, opens door and window and ammonia in mushroom room is left, and maintains mushroom room temperature at 28 DEG C; First evenly be sprinkling upon on substratum charge level by 2/3 bacterial classification, sprinkled by remaining bacterial classification after being covered with the culture base-material of 1cm, with liming to the sterilization of mushroom room, close the doors and windows 4 days, keep mushroom room temperature at 24 DEG C, relative air humidity remains on 80%; If mycelia has started to sprout material feeding after 3d, ventilation should strengthen gradually.After 18 days, be watered with SD-1750 or derosal, sprinkling prevention is carried out to culture base-material and kills disease and pest in material, casingmaterial is carried out disinfection simultaneously, spray clear water and keep casingmaterial water content 40%, 1% lime powder will be admixed in casingmaterial, adjustment casingmaterial potential of hydrogen is 7.8, water content reaches 42% rear earthing, the thick 3.5cm of soil layer, keep mushroom room temperature at 24 DEG C, relative air humidity 87%, cool to 18 DEG C when mycelia grows to soil layer 2/3, spray knot mushroom water, continuous spraying keeps atmospheric moisture 90% for 3 days afterwards; Control room temperature at 16 DEG C, should strengthen more than 22 DEG C ventilation of shading, and earthward and space spray cooling; Winter climate is cold, should notice that insulation heats, and prevents cold wind from invading and causes wind spot mushroom and dead mushroom; Spring temperature gets warm again after a cold spell, and mainly takes cooling measure, extends and produces the mushroom phase, improves Twospore Mushroom output, ventilates every day 2 times, each 2 hours, during low temperature, ventilates when temperature is higher at noon; During high temperature, daytime suitably ventilates less, sooner or later many ventilations, can gather, when adopting mushroom, catch stem to turn round down gently, do not drive too much earthing when cap grows to when 4cm, mycoderm not yet break.Fresh mushroom will be handled with care, to prune mushroom handle base portion with pocket knife, after adopting mushroom, should will be retained in old root that is shrivelled on mushroom bed, that turn yellow in time and dead mushroom is rejected at every turn.After choosing root, with moistening fine earth, the earth taken away when adopting mushroom should be filled in time at every turn, in order to avoid during water spray, in water permeation to culture material, affect mycelial growth.Under criticize that mushroom grows to that soya bean is large, temperature 18 DEG C time, every square metre of culture base-material 0.5 kg of phosphoric acid potassium dihydrogen solution sprays, and potassium dihydrogen phosphate mass percentage concentration is 5 ‰.
Products material acquisition expenses 6937.5 yuan, labour cost 4000 yuan, sends out bacterium speed 1.16cm/ days, sends out 21 days bacterium used times, per unit area yield 1.12kg/m 2.
comparative example 1:
Proportioning takes straw 60 parts by weight, cow dung 35 parts, bagasse 0.5 part, silkworm excrement 1 part, composite fertilizer 1 part, 1 part, lime, 1 part, gypsum, calcium superphosphate 2 parts, cow dung is prewetted 2 days, mix Hou Jiandui with the straw after pulverizing, the turning when piling temperature and declining by 72 DEG C, add when composite fertilizer and bagasse first time turning, calcium superphosphate and gypsum add when second time turning, and lime and silkworm excrement add when third time turning, regulate pH 7.9 after the 4th turning, water content 66%, heap shape is conical.
Also evenly be put on mushroom bed in mushroom room after the culture base-material that heap makes was transported sterilization in one day, close mushroom room door window, mushroom room temperature is maintained at 63 DEG C, kill harmful microorganism and the larva remained in culture material, worm's ovum, pathogenic bacteria and miscellaneous bacteria after 9 hours by ventilating pit; Then top and the bottom ventilation hole is opened, be cooled to 53 DEG C, maintain 4 days, promote that the beneficial microorganisms such as high temperatures actinomycetes are fully bred and Nutrient Transformation, 47 DEG C are cooled to after forming the tropina and various VITAMIN and amino acid easily being absorbed by Twospore Mushroom mycelia in a large number and utilize, keep opening after 12 hours door and window cooling, a large amount of Thermoactinomyces albus throughout the whole substratum bed of material, water content 64%.
Every square metre of culture base-material 500ml/ bottle bacterial classification 2 bottles, prepares enough bacterial classification.Pick the foreign material in culture base-material, leveling charge level, makes bed thickness be 25cm, opens door and window and ammonia in mushroom room is left, and maintains mushroom room temperature at 28 DEG C; First evenly be sprinkling upon on substratum charge level by 2/3 bacterial classification, sprinkled by remaining bacterial classification after being covered with the culture base-material of 1cm, with liming to the sterilization of mushroom room, close the doors and windows 4 days, keep mushroom room temperature at 24 DEG C, relative air humidity remains on 80%; If mycelia has started to sprout material feeding after 3d, ventilation should strengthen gradually.After 18 days, be watered with SD-1750 or derosal, sprinkling prevention is carried out to culture base-material and kills disease and pest in material, casingmaterial is carried out disinfection simultaneously, spray clear water and keep casingmaterial water content 40%, 1% lime powder will be admixed in casingmaterial, adjustment casingmaterial potential of hydrogen is 7.8, water content reaches 42% rear earthing, the thick 3.5cm of soil layer, keep mushroom room temperature at 24 DEG C, relative air humidity 87%, cool to 18 DEG C when mycelia grows to soil layer 2/3, spray knot mushroom water, continuous spraying keeps atmospheric moisture 90% for 3 days afterwards; Control room temperature at 16 DEG C, should strengthen more than 22 DEG C ventilation of shading, and earthward and space spray cooling; Winter climate is cold, should notice that insulation heats, and prevents cold wind from invading and causes wind spot mushroom and dead mushroom; Spring temperature gets warm again after a cold spell, and mainly takes cooling measure, extends and produces the mushroom phase, improves Twospore Mushroom output, ventilates every day 2 times, each 2 hours, during low temperature, ventilates when temperature is higher at noon; During high temperature, daytime suitably ventilates less, sooner or later many ventilations, can gather, when adopting mushroom, catch stem to turn round down gently, do not drive too much earthing when cap grows to when 4cm, mycoderm not yet break.Fresh mushroom will be handled with care, to prune mushroom handle base portion with pocket knife, after adopting mushroom, should will be retained in old root that is shrivelled on mushroom bed, that turn yellow in time and dead mushroom is rejected at every turn.After choosing root, with moistening fine earth, the earth taken away when adopting mushroom should be filled in time at every turn, in order to avoid during water spray, in water permeation to culture material, affect mycelial growth.Under criticize that mushroom grows to that soya bean is large, temperature 18 DEG C time, every square metre of culture base-material 0.5 kg of phosphoric acid potassium dihydrogen solution sprays, and potassium dihydrogen phosphate mass percentage concentration is 5 ‰.
Products material acquisition expenses 8977.5 yuan, labour cost 6500 yuan, sends out bacterium speed 1.05cm/ days, sends out 23 days bacterium used times, per unit area yield 1.05kg/m 2.
comparative example 2:
Proportioning takes needle mushroom dreg 30 parts by weight, straw 30 parts, cow dung 35 parts, bagasse 0.5 part, silkworm excrement 1 part, composite fertilizer 1 part, 1 part, lime, 1 part, gypsum, calcium superphosphate 2 parts, by cow dung, straw is prewetted 3 days, Hou Jiandui is mixed with needle mushroom dreg, the turning when piling temperature and declining by 75 DEG C, add when composite fertilizer and bagasse first time turning, calcium superphosphate and gypsum add when second time turning, lime and silkworm excrement add when third time turning, after 4th turning, culture base-material is light coffee color, there is material fragrance, slightly ammonia odor, regulate pH 7.9, water content 66%, heap shape is conical.
Also evenly be put on mushroom bed in mushroom room after the culture base-material that heap makes was transported sterilization in one day, close mushroom room door window, mushroom room temperature is maintained at 64 DEG C, kill harmful microorganism and the larva remained in culture material, worm's ovum, pathogenic bacteria and miscellaneous bacteria after 9 hours by ventilating pit; Then top and the bottom ventilation hole is opened, be cooled to 50 DEG C, maintain 4 days, promote that the beneficial microorganisms such as high temperatures actinomycetes are fully bred and Nutrient Transformation, 45 DEG C are cooled to after forming the tropina and various VITAMIN and amino acid easily being absorbed by Twospore Mushroom mycelia in a large number and utilize, keep after 12 hours, opening door and window cooling, a large amount of Thermoactinomyces albus is throughout the whole substratum bed of material, culture base-material is dark brown, without ammonia stink, there is material fragrance, culture base-material is soft, good permeability, water content 63%.
Every square metre of culture base-material 500ml/ bottle bacterial classification 2 bottles, prepares enough bacterial classification.Pick the foreign material in culture base-material, leveling charge level, makes bed thickness be 25cm, opens door and window and ammonia in mushroom room is left, and maintains mushroom room temperature at 28 DEG C; First evenly be sprinkling upon on substratum charge level by 2/3 bacterial classification, sprinkled by remaining bacterial classification after being covered with the culture base-material of 1cm, with liming to the sterilization of mushroom room, close the doors and windows 4 days, keep mushroom room temperature at 24 DEG C, relative air humidity remains on 80%; If mycelia has started to sprout material feeding after 3d, ventilation should strengthen gradually.After 18 days, be watered with SD-1750 or derosal, sprinkling prevention is carried out to culture base-material and kills disease and pest in material, casingmaterial is carried out disinfection simultaneously, spray clear water and keep casingmaterial water content 40%, 1% lime powder will be admixed in casingmaterial, adjustment casingmaterial potential of hydrogen is 7.8, water content reaches 42% rear earthing, the thick 3.5cm of soil layer, keep mushroom room temperature at 24 DEG C, relative air humidity 87%, cool to 18 DEG C when mycelia grows to soil layer 2/3, spray knot mushroom water, continuous spraying keeps atmospheric moisture 90% for 3 days afterwards; Control room temperature at 16 DEG C, should strengthen more than 22 DEG C ventilation of shading, and earthward and space spray cooling; Winter climate is cold, should notice that insulation heats, and prevents cold wind from invading and causes wind spot mushroom and dead mushroom; Spring temperature gets warm again after a cold spell, and mainly takes cooling measure, extends and produces the mushroom phase, improves Twospore Mushroom output, ventilates every day 2 times, each 2 hours, during low temperature, ventilates when temperature is higher at noon; During high temperature, daytime suitably ventilates less, sooner or later many ventilations, can gather, when adopting mushroom, catch stem to turn round down gently, do not drive too much earthing when cap grows to when 4cm, mycoderm not yet break.Fresh mushroom will be handled with care, to prune mushroom handle base portion with pocket knife, after adopting mushroom, should will be retained in old root that is shrivelled on mushroom bed, that turn yellow in time and dead mushroom is rejected at every turn.After choosing root, with moistening fine earth, the earth taken away when adopting mushroom should be filled in time at every turn, in order to avoid during water spray, in water permeation to culture material, affect mycelial growth.Under criticize that mushroom grows to that soya bean is large, temperature 18 DEG C time, every square metre of culture base-material 0.5 kg of phosphoric acid potassium dihydrogen solution sprays, and potassium dihydrogen phosphate mass percentage concentration is 5 ‰.
Products material acquisition expenses 7939.5 yuan, labour cost 5970 yuan, sends out bacterium speed 1.1cm/ days, sends out 22 days bacterium used times, per unit area yield 1.08kg/m 2.
comparative example 3:
Proportioning takes needle mushroom dreg 40 parts by weight, straw 20 parts, cow dung 35 parts, bagasse 0.5 part, silkworm excrement 1 part, composite fertilizer 1 part, 1 part, lime, 1 part, gypsum, calcium superphosphate 2 parts, by cow dung, straw is prewetted 3 days, Hou Jiandui is mixed with needle mushroom dreg, the turning when piling temperature and declining by 75 DEG C, add when composite fertilizer and bagasse first time turning, calcium superphosphate and gypsum add when second time turning, lime and silkworm excrement add when third time turning, after 4th turning, culture base-material is light coffee color, there is material fragrance, slightly ammonia odor, regulate pH 7.9, water content 66%, heap shape is conical.
Also evenly be put on mushroom bed in mushroom room after the culture base-material that heap makes was transported sterilization in one day, close mushroom room door window, mushroom room temperature is maintained at 64 DEG C, kill harmful microorganism and the larva remained in culture material, worm's ovum, pathogenic bacteria and miscellaneous bacteria after 9 hours by ventilating pit; Then top and the bottom ventilation hole is opened, be cooled to 50 DEG C, maintain 4 days, promote that the beneficial microorganisms such as high temperatures actinomycetes are fully bred and Nutrient Transformation, 45 DEG C are cooled to after forming the tropina and various VITAMIN and amino acid easily being absorbed by Twospore Mushroom mycelia in a large number and utilize, keep after 12 hours, opening door and window cooling, a large amount of Thermoactinomyces albus is throughout the whole substratum bed of material, culture base-material is dark brown, without ammonia stink, there is material fragrance, culture base-material is soft, good permeability, water content 63%.
Every square metre of culture base-material 500ml/ bottle bacterial classification 2 bottles, prepares enough bacterial classification.Pick the foreign material in culture base-material, leveling charge level, makes bed thickness be 25cm, opens door and window and ammonia in mushroom room is left, and maintains mushroom room temperature at 28 DEG C; First evenly be sprinkling upon on substratum charge level by 2/3 bacterial classification, sprinkled by remaining bacterial classification after being covered with the culture base-material of 1cm, with liming to the sterilization of mushroom room, close the doors and windows 4 days, keep mushroom room temperature at 24 DEG C, relative air humidity remains on 80%; If mycelia has started to sprout material feeding after 3d, ventilation should strengthen gradually.After 18 days, be watered with SD-1750 or derosal, sprinkling prevention is carried out to culture base-material and kills disease and pest in material, casingmaterial is carried out disinfection simultaneously, spray clear water and keep casingmaterial water content 40%, 1% lime powder will be admixed in casingmaterial, adjustment casingmaterial potential of hydrogen is 7.8, water content reaches 42% rear earthing, the thick 3.5cm of soil layer, keep mushroom room temperature at 24 DEG C, relative air humidity 87%, cool to 18 DEG C when mycelia grows to soil layer 2/3, spray knot mushroom water, continuous spraying keeps atmospheric moisture 90% for 3 days afterwards; Control room temperature at 16 DEG C, should strengthen more than 22 DEG C ventilation of shading, and earthward and space spray cooling; Winter climate is cold, should notice that insulation heats, and prevents cold wind from invading and causes wind spot mushroom and dead mushroom; Spring temperature gets warm again after a cold spell, and mainly takes cooling measure, extends and produces the mushroom phase, improves Twospore Mushroom output, ventilates every day 2 times, each 2 hours, during low temperature, ventilates when temperature is higher at noon; During high temperature, daytime suitably ventilates less, sooner or later many ventilations, can gather, when adopting mushroom, catch stem to turn round down gently, do not drive too much earthing when cap grows to when 4cm, mycoderm not yet break.Fresh mushroom will be handled with care, to prune mushroom handle base portion with pocket knife, after adopting mushroom, should will be retained in old root that is shrivelled on mushroom bed, that turn yellow in time and dead mushroom is rejected at every turn.After choosing root, with moistening fine earth, the earth taken away when adopting mushroom should be filled in time at every turn, in order to avoid during water spray, in water permeation to culture material, affect mycelial growth.Under criticize that mushroom grows to that soya bean is large, temperature 18 DEG C time, every square metre of culture base-material 0.5 kg of phosphoric acid potassium dihydrogen solution sprays, and potassium dihydrogen phosphate mass percentage concentration is 5 ‰.
Products material acquisition expenses 7507.5 yuan, labour cost 5550 yuan, sends out bacterium speed 1.12cm/ days, sends out 22 days bacterium used times, per unit area yield 1.09kg/m 2.
comparative example 4:
Proportioning ratio takes needle mushroom dreg 50 parts by weight, straw 10 parts, cow dung 35 parts, bagasse 0.5 part, silkworm excrement 1 part, composite fertilizer 1 part, 1 part, lime, 1 part, gypsum, calcium superphosphate 2 parts, by cow dung, straw is prewetted 3 days, Hou Jiandui is mixed with needle mushroom dreg, the turning when piling temperature and declining by 75 DEG C, add when composite fertilizer and bagasse first time turning, calcium superphosphate and gypsum add when second time turning, lime and silkworm excrement add when third time turning, after 4th turning, culture base-material is light coffee color, there is material fragrance, slightly ammonia odor, regulate pH 7.9, water content 66%, heap shape is conical.
Also evenly be put on mushroom bed in mushroom room after the culture base-material that heap makes was transported sterilization in one day, close mushroom room door window, mushroom room temperature is maintained at 64 DEG C, kill harmful microorganism and the larva remained in culture material, worm's ovum, pathogenic bacteria and miscellaneous bacteria after 9 hours by ventilating pit; Then top and the bottom ventilation hole is opened, be cooled to 50 DEG C, maintain 4 days, promote that the beneficial microorganisms such as high temperatures actinomycetes are fully bred and Nutrient Transformation, 45 DEG C are cooled to after forming the tropina and various VITAMIN and amino acid easily being absorbed by Twospore Mushroom mycelia in a large number and utilize, keep after 12 hours, opening door and window cooling, a large amount of Thermoactinomyces albus is throughout the whole substratum bed of material, culture base-material is dark brown, without ammonia stink, there is material fragrance, culture base-material is soft, good permeability, water content 63%.
Every square metre of culture base-material 500ml/ bottle bacterial classification 2 bottles, prepares enough bacterial classification.Pick the foreign material in culture base-material, leveling charge level, makes bed thickness be 25cm, opens door and window and ammonia in mushroom room is left, and maintains mushroom room temperature at 28 DEG C; First evenly be sprinkling upon on substratum charge level by 2/3 bacterial classification, sprinkled by remaining bacterial classification after being covered with the culture base-material of 1cm, with liming to the sterilization of mushroom room, close the doors and windows 4 days, keep mushroom room temperature at 24 DEG C, relative air humidity remains on 80%; If mycelia has started to sprout material feeding after 3d, ventilation should strengthen gradually.After 18 days, be watered with SD-1750 or derosal, sprinkling prevention is carried out to culture base-material and kills disease and pest in material, casingmaterial is carried out disinfection simultaneously, spray clear water and keep casingmaterial water content 40%, 1% lime powder will be admixed in casingmaterial, adjustment casingmaterial potential of hydrogen is 7.8, water content reaches 42% rear earthing, the thick 3.5cm of soil layer, keep mushroom room temperature at 24 DEG C, relative air humidity 87%, cool to 18 DEG C when mycelia grows to soil layer 2/3, spray knot mushroom water, continuous spraying keeps atmospheric moisture 90% for 3 days afterwards; Control room temperature at 16 DEG C, should strengthen more than 22 DEG C ventilation of shading, and earthward and space spray cooling; Winter climate is cold, should notice that insulation heats, and prevents cold wind from invading and causes wind spot mushroom and dead mushroom; Spring temperature gets warm again after a cold spell, and mainly takes cooling measure, extends and produces the mushroom phase, improves Twospore Mushroom output, ventilates every day 2 times, each 2 hours, during low temperature, ventilates when temperature is higher at noon; During high temperature, daytime suitably ventilates less, sooner or later many ventilations, can gather, when adopting mushroom, catch stem to turn round down gently, do not drive too much earthing when cap grows to when 4cm, mycoderm not yet break.Fresh mushroom will be handled with care, to prune mushroom handle base portion with pocket knife, after adopting mushroom, should will be retained in old root that is shrivelled on mushroom bed, that turn yellow in time and dead mushroom is rejected at every turn.After choosing root, with moistening fine earth, the earth taken away when adopting mushroom should be filled in time at every turn, in order to avoid during water spray, in water permeation to culture material, affect mycelial growth.Under criticize that mushroom grows to that soya bean is large, temperature 18 DEG C time, every square metre of culture base-material 0.5 kg of phosphoric acid potassium dihydrogen solution sprays, and potassium dihydrogen phosphate mass percentage concentration is 5 ‰.
Products material acquisition expenses 7297.5 yuan, labour cost 4300 yuan, sends out bacterium speed 1.13cm/ days, sends out 21 days bacterium used times, per unit area yield 1.14kg/m 2.
Learnt by test: 1, every standard canopy room can economize in raw materials and purchase production cost 1038-2040 unit, and wherein embodiment 1 compares with comparative example 1, cost-savingly can reach 22.72%;
2, after utilizing bacterium slag to substitute forage, send out compared with the bacterium time cultivates with forage with in fruiting density at Twospore Mushroom, mycelia material feeding sprout time in advance, send out that bacterium speed is fast, mycelial growth is healthy and strong, budding and fruiting density high;
3, after utilizing bacterium slag to substitute, different culture material proportionings fruiting time of buddingging, winter stop growing time and output have certain difference, fruiting time that can make to budding does sth. in advance 3-5 days, be conducive to Twospore Mushroom product to go on the market in advance, and extend fruiting in winter time 7-10 days, increase the output 0.03-0.09 kg/m of Twospore Mushroom 2, improve the biological transformation ratio of culturing raw material and the economic benefit of cultivation of agaricus bisporus.
All components in above embodiment all can business be bought.
Above-described embodiment is just for setting forth content of the present invention, instead of restriction, and any change therefore in the implication suitable with claims of the present invention and scope, all should think to be included in the scope of claims.

Claims (5)

1. a needle mushroom dreg culture base-material, it is characterized in that, the raw materials by weight portion proportioning of described needle mushroom dreg culture base-material is as follows: needle mushroom dreg 30-60 part, cow dung 35 parts, bagasse 0.5-1 part, silkworm excrement 0.5-1.5 part, composite fertilizer 1 part, lime 1-1.5 part, gypsum 0.5-1 part, calcium superphosphate 2 parts.
2. utilize a method for the cultivating bisporous mushroom of needle mushroom dreg culture base-material described in claim 1, it is characterized in that, comprise the steps:
The first step: needle mushroom dreg is pulverized, dries rear for subsequent use;
Second step: prewet cow dung 2-3 days, Hou Jiandui is mixed with needle mushroom dreg, the turning when piling temperature and declining by 70-75 DEG C, add when composite fertilizer and bagasse first time turning, calcium superphosphate and gypsum add when second time turning, and lime and silkworm excrement add when third time turning, regulate pH at 7.8-8 after the 4th turning, water content 65%-67%, obtained needle mushroom dreg culture base-material;
3rd step: the needle mushroom dreg culture base-material that heap makes is transported in the mushroom room after sterilization and is also evenly put on mushroom bed, close mushroom room door window, mushroom room temperature is maintained at 62-65 DEG C by ventilating pit, 50-55 DEG C is cooled to after 8-10 hour, maintain and be cooled to 45-50 DEG C after 3-4 days, keep after 12 hours, opening door and window cooling;
4th step: pick the foreign material in needle mushroom dreg culture base-material, leveling charge level, makes bed thickness be 20-25cm, opens door and window and ammonia in mushroom room is left, and maintains mushroom room temperature at 22-28 DEG C;
5th step: first 2/3 bacterial classification is evenly sprinkling upon on needle mushroom dreg substratum charge level, after being covered with the needle mushroom dreg culture base-material of 0.5-1cm, remaining bacterial classification is sprinkled, with liming, sterilized in mushroom room, close the doors and windows 3-5 days, keep mushroom room temperature at 22-26 DEG C, relative air humidity remains on 75-85%;
After 6th step: 15-20 days, be watered with SD-1750 or derosal, sprinkling prevention is carried out to needle mushroom dreg culture base-material and kills disease and pest in material, 1% lime powder will be admixed in casingmaterial, adjustment casingmaterial potential of hydrogen is 7.5-8, water content reaches earthing after 40-45%, the thick 3-3.5cm of soil layer, keep mushroom room temperature at 22-26 DEG C, relative air humidity 85-90%, cool to 15-20 DEG C when mycelia grows to soil layer 2/3, spray knot mushroom water, kept atmospheric moisture at 85%-95% after continuous spraying 2-3 days;
7th step: control room temperature at 15-18 DEG C, every day ventilates 1-2 time, each 1-2 hour, can gather when cap grows to when 3-4cm, mycoderm not yet break.
3. the method for the cultivating bisporous mushroom of a kind of needle mushroom dreg culture base-material according to claim 2, is characterized in that: the quantity of described 5th step bacterial classification is every square metre of culture base-material 500ml/ bottle bacterial classification 1.5-2 bottle.
4. the method for the cultivating bisporous mushroom of a kind of needle mushroom dreg culture base-material according to claim 2, is characterized in that: will carry out disinfection to casingmaterial before described 6th step earthing, sprays clear water simultaneously and keeps casingmaterial water content 40%.
5. the method for the cultivating bisporous mushroom of a kind of needle mushroom dreg culture base-material according to claim 2, it is characterized in that: after adopting mushroom in described 7th step lower batch mushroom grow to that soya bean is large, temperature 18 DEG C time, every square metre of culture base-material 0.5 kg of phosphoric acid potassium dihydrogen solution sprays, and potassium dihydrogen phosphate mass percentage concentration is 5 ‰.
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