CN106172554A - A kind of plant extract for evoking tobacco generation to powder mildew resistance - Google Patents
A kind of plant extract for evoking tobacco generation to powder mildew resistance Download PDFInfo
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- CN106172554A CN106172554A CN201610544509.1A CN201610544509A CN106172554A CN 106172554 A CN106172554 A CN 106172554A CN 201610544509 A CN201610544509 A CN 201610544509A CN 106172554 A CN106172554 A CN 106172554A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
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Abstract
The invention discloses and a kind of produce the preparation method to the plant extract of powder mildew resistance for evoking tobacco: the preparation of (1) Rhizoma Zingiberis Recens extract；(2) preparation of soapberry extract；(3) preparation of sea coconut extractive；(4) preparation of Folium Eriobotryae extract；(5) according to parts by weight, take Rhizoma Zingiberis Recens extract 5 10 parts, soapberry extract 35 parts, sea coconut extractive 23 parts, ground Folium Eriobotryae extract 35 parts, pure water 120 parts, be mixed to get and produce the plant extract to powder mildew resistance for evoking tobacco.The using method of above-mentioned plant extract is: the plant extract aerosol apparatus of the present invention is sprayed in the tobacco plant of 13 leaves in field, the effect of the " Invest, Then Investigate " induction of resistance of 10 days.Result: induction of resistance active effect is 70.2 92.3%.
Description
Technical field
The invention belongs to the present invention relates to plant pest green ecological Prevention Technique field, particularly one is used for inducing
Nicotiana tabacum L. produces the plant extract to powder mildew resistance.
Background technology
Powdery Mildew in Tobacco is commonly called as " upper ash ", " frost occurs ", " upper nitre ".This disease causes harm ripe Lao Ye more, develops from bottom to top.Leaf
Sheet is fallen ill, and is just rotund yellowish-brown stigma, and existing white powder point on rear speckle, making scab is blanket, and speckle expands, white lead
It is covered with whole blade, sick leaf elder generation chlorisis browning, rear withered.Cause harm time serious tender stem, sick stem is also covered with white lead.After sick leaf baking
Thin as a piece of paper, color secretly becomes rusty brown, loses economic worth.All there is a generation in Ge Yan district of China, wherein with south China, southwest and Central China Yan Qu
For generally, endanger the most heavier.
Cause of disease is Powdery Mildew in Tobacco bacterium, belongs to Ascomycotina, gang pyrenomycetes, Erysiphales, Erysiphe.Seedling stage and Adult plant
All can occur.
Powdery Mildew in Tobacco is infected caused by the tobacco powder spore bacterium of Deuteromycotina.Its Perfect stage is then included into Ascomycotina
Two spore powdery mildews, but Perfect stage seldom finds.Pathogenic bacteria mycelia tool separates, colourless.The conidiophore of Invisible element hangs down with mycelia
Directly, thread, shorter, without branch, size about 80～120 × 12～14 microns, acroconidium.Conidium is concatenated, by upper
Under and sequentially ripe, colourless, unit cell, cylindrical shape, size is 30～32 × 13～15 microns.Pathogen growth thermophilic 22 DEG C～28
℃.Conidia germination optimum temperature 23 DEG C～25 DEG C, the suitableeest relative humidity 60%～80%.At relative humidity less than 20% still
There is minority spore to sprout, but seldom can sprout in relative humidity 100% or water droplet.Pathogenic bacteria belongs to obligate ectophyte,
All mycelium length is at host surfaces, draws nutrient in stretching into epidermal cells of host with hapteron.It is existing to there is Physiological Differentiation in pathogenic bacteria
As, there is the biological strain of many different pathological forms, they are widely different to the pathogenicity of different hosts.Pathogenic bacteria host range is wide,
Outside smoke abatement grass, moreover it is possible to infect 100 various plants such as Cucurbitaceae, Compositae.
Prior art is disclosed the induction mildew-resistance activity that a kind of Bulbus Eleutherines Plicatae extract produces on tobacco plant, but should
Method induction of resistance active effect is the best.
Producing the plant extract to powder mildew resistance for evoking tobacco it is desirable to provide a kind of, this product is with existing
There is technology to compare, induction mildew-resistance active effect can be effectively improved, and consumption is few, cost-effective.
Summary of the invention
It is an object of the invention to provide a kind of plant extract for evoking tobacco generation to powder mildew resistance, this product
Compared with prior art, induction mildew-resistance active effect can be effectively improved, and consumption is few, cost-effective.
In order to realize above-mentioned purpose, present invention employs following technical scheme:
A kind of preparation method producing the plant extract to powder mildew resistance for evoking tobacco, it is characterised in that step is such as
Under:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of sea coconut extractive, dries sea Cortex cocois radicis, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of Folium Eriobotryae extract, dries ground loquat, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) according to parts by weight, Rhizoma Zingiberis Recens extract 5-10 part, soapberry extract 3-5 part, sea coconut extractive 2-3 part, ground are taken
Folium Eriobotryae extract 3-5 part, pure water 120 parts, it is mixed to get and produces the plant extract to powder mildew resistance for evoking tobacco.
The using method of above-mentioned plant extract is: the plant extract aerosol apparatus of the present invention is sprayed in 13, field
The tobacco plant of leaf, the effect of the " Invest, Then Investigate " induction of resistance of 10 days.
Induction of resistance active effect (%)=(comparison lesion area-process lesion area)/comparison lesion area × 100.
The invention have benefit that:
The present invention provides a kind of and produces the plant extract to powder mildew resistance, this product and prior art phase for evoking tobacco
Ratio, can be effectively improved induction mildew-resistance active effect, and consumption is few, cost-effective.
Detailed description of the invention
The microbial inoculum of the present invention is commercially available acquisition.
Embodiment 1:
A kind of preparation method producing the plant extract to powder mildew resistance for evoking tobacco, it is characterised in that step is such as
Under:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of sea coconut extractive, dries sea Cortex cocois radicis, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of Folium Eriobotryae extract, dries ground loquat, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) according to parts by weight, taking Rhizoma Zingiberis Recens extract 5 parts, soapberry extract 5 parts, sea coconut extractive 2 parts, ground Folium Eriobotryae carries
Take thing 5 parts, pure water 120 parts, be mixed to get and produce the plant extract to powder mildew resistance for evoking tobacco.
The using method of above-mentioned plant extract is: the plant extract aerosol apparatus of the present invention is sprayed in 13, field
The tobacco plant of leaf, the effect of the " Invest, Then Investigate " induction of resistance of 10 days.
Result: induction of resistance active effect is 70.2%.
Embodiment 2:
A kind of preparation method producing the plant extract to powder mildew resistance for evoking tobacco, it is characterised in that step is such as
Under:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of sea coconut extractive, dries sea Cortex cocois radicis, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of Folium Eriobotryae extract, dries ground loquat, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) according to parts by weight, taking Rhizoma Zingiberis Recens extract 10 parts, soapberry extract 3 parts, sea coconut extractive 3 parts, ground Folium Eriobotryae carries
Take thing 3 parts, pure water 120 parts, be mixed to get and produce the plant extract to powder mildew resistance for evoking tobacco.
The using method of above-mentioned plant extract is: the plant extract aerosol apparatus of the present invention is sprayed in 13, field
The tobacco plant of leaf, the effect of the " Invest, Then Investigate " induction of resistance of 10 days.
Result: induction of resistance active effect is 92.3%.
Embodiment 3:
A kind of preparation method producing the plant extract to powder mildew resistance for evoking tobacco, it is characterised in that step is such as
Under:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of sea coconut extractive, dries sea Cortex cocois radicis, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of Folium Eriobotryae extract, dries ground loquat, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) according to parts by weight, taking Rhizoma Zingiberis Recens extract 6 parts, soapberry extract 4 parts, sea coconut extractive 2 parts, ground Folium Eriobotryae carries
Take thing 4 parts, pure water 120 parts, be mixed to get and produce the plant extract to powder mildew resistance for evoking tobacco.
The using method of above-mentioned plant extract is: the plant extract aerosol apparatus of the present invention is sprayed in 13, field
The tobacco plant of leaf, the effect of the " Invest, Then Investigate " induction of resistance of 10 days.
Result: induction of resistance active effect is 80.6%.
Embodiment 4:
A kind of preparation method producing the plant extract to powder mildew resistance for evoking tobacco, it is characterised in that step is such as
Under:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of sea coconut extractive, dries sea Cortex cocois radicis, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of Folium Eriobotryae extract, dries ground loquat, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) according to parts by weight, taking Rhizoma Zingiberis Recens extract 9 parts, soapberry extract 3 parts, sea coconut extractive 3 parts, ground Folium Eriobotryae carries
Take thing 5 parts, pure water 120 parts, be mixed to get and produce the plant extract to powder mildew resistance for evoking tobacco.
The using method of above-mentioned plant extract is: the plant extract aerosol apparatus of the present invention is sprayed in 13, field
The tobacco plant of leaf, the effect of the " Invest, Then Investigate " induction of resistance of 10 days.
Result: induction of resistance active effect is 75.9%.
Embodiment 5:
A kind of preparation method producing the plant extract to powder mildew resistance for evoking tobacco, it is characterised in that step is such as
Under:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of sea coconut extractive, dries sea Cortex cocois radicis, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of Folium Eriobotryae extract, dries ground loquat, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) according to parts by weight, taking Rhizoma Zingiberis Recens extract 7 parts, soapberry extract 5 parts, sea coconut extractive 2 parts, ground Folium Eriobotryae carries
Take thing 3 parts, pure water 120 parts, be mixed to get and produce the plant extract to powder mildew resistance for evoking tobacco.
The using method of above-mentioned plant extract is: the plant extract aerosol apparatus of the present invention is sprayed in 13, field
The tobacco plant of leaf, the effect of the " Invest, Then Investigate " induction of resistance of 10 days.
Result: induction of resistance active effect is 90.7%.
Visible, the present invention provides a kind of and produces plant extract to powder mildew resistance for evoking tobacco, this product with
Prior art is compared, and can be effectively improved induction mildew-resistance active effect, and consumption is few, cost-effective.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope that the invention discloses, according to technical scheme and
Inventive concept equivalent or change in addition, all should contain within protection scope of the present invention.
Claims (3)
1. the preparation method producing the plant extract to powder mildew resistance for evoking tobacco, it is characterised in that step is such as
Under:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of sea coconut extractive, dries sea Cortex cocois radicis, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of Folium Eriobotryae extract, dries ground loquat, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) Rhizoma Zingiberis Recens extract is taken, soapberry extract, sea coconut extractive, ground Folium Eriobotryae extract, pure water 120 parts, mix
To producing the plant extract to powder mildew resistance for evoking tobacco.
2. the preparation method producing the plant extract to powder mildew resistance for evoking tobacco described in claim 1, it is special
Levy and be:
Described step (5) is: according to parts by weight, takes Rhizoma Zingiberis Recens extract 5-10 part, soapberry extract 3-5 part, and sea Cortex cocois radicis carries
Take thing 2-3 part, ground Folium Eriobotryae extract 3-5 part, pure water 120 parts, be mixed to get and produce powder mildew resistance for evoking tobacco
Plant extract.
3. the evoking tobacco that the method described in claim 1-2 prepares produces the plant extract to powder mildew resistance.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610544509.1A CN106172554A (en) | 2016-07-12 | 2016-07-12 | A kind of plant extract for evoking tobacco generation to powder mildew resistance |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610544509.1A CN106172554A (en) | 2016-07-12 | 2016-07-12 | A kind of plant extract for evoking tobacco generation to powder mildew resistance |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114617137A (en) * | 2022-04-07 | 2022-06-14 | 华植河北生物科技有限公司 | Pesticide composition and preparation for treating tobacco powdery mildew and black shank and application thereof |
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| CN103651617A (en) * | 2013-12-04 | 2014-03-26 | 云南省农业科学院农业环境资源研究所 | Plant extract for inducing tobacco to generate resistance to powdery mildew and preparation method thereof |
| CN104489001A (en) * | 2014-12-04 | 2015-04-08 | 苏州市新巷农艺科技园 | Preparation method of universal pesticide for flowers |
| CN105145691A (en) * | 2015-09-18 | 2015-12-16 | 张滋柠 | Household preparation and use method of biological insecticidal bacteriostatic agent |
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2016
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Patent Citations (3)
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| CN103651617A (en) * | 2013-12-04 | 2014-03-26 | 云南省农业科学院农业环境资源研究所 | Plant extract for inducing tobacco to generate resistance to powdery mildew and preparation method thereof |
| CN104489001A (en) * | 2014-12-04 | 2015-04-08 | 苏州市新巷农艺科技园 | Preparation method of universal pesticide for flowers |
| CN105145691A (en) * | 2015-09-18 | 2015-12-16 | 张滋柠 | Household preparation and use method of biological insecticidal bacteriostatic agent |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114617137A (en) * | 2022-04-07 | 2022-06-14 | 华植河北生物科技有限公司 | Pesticide composition and preparation for treating tobacco powdery mildew and black shank and application thereof |
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