CN103918813A - National bouquet golden camellia tea and method for preparing national bouquet golden camellia tea - Google Patents
National bouquet golden camellia tea and method for preparing national bouquet golden camellia tea Download PDFInfo
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Abstract
The invention belongs to the technical field of food and particularly relates to national bouquet golden camellia tea and a method for preparing the national bouquet golden camellia tea. The national bouquet golden camellia tea is composed of, by weight, 1-99.99 parts of tea leaves, 0.01-10 parts of golden flower fungi, and 0.01-10 parts of Maotai aroma microorganisms or sauce flavor generating fungi. The invention further provides a method for preparing the national bouquet golden camellia tea. The national bouquet golden camellia tea prepared with the method has mellow and thick bouquet and contains physiological activators, such as tea polysaccharide, in golden camellia, the health pursuit of modern people can be met, and the bouquet is provided while people drink golden camellia tea.
Description
Technical field
The invention belongs to food technology field, be specifically related to a kind of state aroma Camellia nitidissima and preparation method thereof.
Background technology
Camellia nitidissima is a kind of ancient plant; very rare; distribute extremely narrow; the wild Camellia nitidissima in the whole world 90% is only distributed in blue mountain offshoot one band of China's tourist resources's Shiwan Dashan; one of eight kinds of first-grade state protection plants of China (1984.7 State Council's files); belong to the plant species in < < CITS > > appendix II; be referred to as magical " Dongfang " magic tea abroad, be described as " plant kingdom giant panda ", " queen of tea family ".
Since Camellia nitidissima is found, the mensuration of the authoritative department of process modern medicine and relevant scientific research institution: the nontoxic level of golden flower Camellia, the trace elements such as the germanium being rich in (Ge), selenium (Se), zinc (Zn), cobalt (Co), molybdenum (Mo), vanadium (V) have important health-care effect to human body.Golden camellia tea also contains the beneficiating ingredient of the multiple needed by human body such as several amino acids, Tea Polyphenols, saponin class, flavonoids; In vitro Bactericidal Experiments shows, Camellia nitidissima scope of restraining fungi is wide, and diphtheria bacterium, shigella dysenteriae, Pseudomonas aeruginosa and typhoid bacillus are had to good inhibitory action.In addition, Camellia nitidissima proves in animal experiment, it suppresses the effect of malignant tumour in addition, and in the contrast test of norcholesterol reducing blood lipid, Camellia nitidissima is all better than existing Western medicine " clofibrate " to the reduced rate of serum total cholesterol and serum beta lipoprotein, therefore, the DEVELOPMENT PROSPECT of Camellia nitidissima is very tempting.
Summary of the invention
The object of the invention is to utilize fermentation technique that a kind of state aroma Camellia nitidissima and manufacture method thereof are provided.Under process conditions of the present invention, make the state aroma Camellia nitidissima that not only there is mellow aroma but also retain the physiological activators such as tea polysaccharide in Camellia nitidissima.This kind of Camellia nitidissima meets modern to healthy pursuit, can in drink Camellia nitidissima, also meet the needs to aroma.
The present invention is achieved through the following technical solutions.
A state aroma Camellia nitidissima, is characterized in that by 1-99.99 weight portion tealeaves, 0.01-10 weight portion golden flower bacterium, 0.01-10 weight portion Maotai producing mellow microorganism or produces the fragrant function bacterium of sauce forming.
Described tealeaves is that fresh tea or one-tenth are sampled tea.
Described Maotai producing mellow microorganism is for producing one or more compositions in the Aspergillus obtaining with separation and purification Daqu, white mucor, rhizopus, pears head mould, false yeast, endomycopsis, Hansenula yeast, lactic acid bacteria, acetic acid bacteria, bacillus licheniformis, bacillus subtilis, bacillus alcalophilus, cured shape bacillus, bacillus pumilus, bacillus amyloliquefaciens, bafillus natto from Maotai.
Described golden flower bacterium for separation and purification obtains from Pu'er tea or Fu brick tea coronoid process loose capsule bacterium, E.amstelodami, E.intermedius, thank to one or more in a watt loose capsule bacterium, E.repens, Eurotium costiforme, the loose capsule bacterium of wax leaf, red loose capsule bacterium.
The preparation method of a kind of state aroma Camellia nitidissima:
Step 1: steam tealeaves with high-temperature steam and carry out sterilizing for 1-10 minute and make it softening, controlled humidity is 20-30% simultaneously; The ratio that golden flower bacterium bacterium liquid is added to 0.1-5mL according to every 100g tea sprays on tealeaves equably, and the tealeaves after fully mixing dries 1-90 minute in 40-70 ℃ in confined conditions; And then will use the resuspended bacterium liquid of Maotai producing mellow microorganism of nutrient solution to be evenly sprayed onto on tealeaves; Described nutrient solution is one or more compositions of Fructus Rosae Laevigatae extract, compound amino acid, vitamin, trace element, glucose, wood sugar, sucrose, fructose, aseptic deionized water;
Step 2: by above-mentioned tealeaves at humidity 30-90%, temperature 20-40 ℃ and aseptic condition bottom fermentation;
Step 3: use causes fragrant liquid the fermented tea of gained is fumigated to 0.1-90 hour under aseptic or open condition, after stifling, under 20-220 ℃ of condition, cure 2 seconds-48 hours, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak case or oak barrel that causes fragrant liquid generator, after airtight ageing 1-300 days, obtain state aroma golden flower bacterium fermented tea finished product, can make tea cake through steam pressure; It is described that to cause fragrant liquid be Maotai producing mellow microorganism or produce one or more compositions in fermentation, extraction thing in amino acid and reduced sugar of the fragrant function bacterium metabolite of sauce, Maotai producing mellow microorganism, Maotai, Fructus Rosae Laevigatae extract.
The preparation method of a kind of state aroma Camellia nitidissima:
Step 1: the pass-through type that the fresh leaves of tea plant of harvesting is carried out 3-7 hour withers, presents after wilted condition in the 60-250 ℃ of 1-30 minute that completes fresh tea, carries out 10-50 minute knead after cooling to obtain kneading leaf;
Step 2: will knead leaf under air-proof condition under 121 ℃ of high steams after sterilizing 1-30 minute, by the amount inoculation 0.1-5ml golden flower bacterium bacterium liquid of every 100g sterilizing tealeaves, cultivation and fermentation 1-10 days under temperature 20-50 ℃, humidity 50-70%, obtains golden flower bacterium tea;
Step 3: after the wet thallus of Maotai producing mellow microorganism, pulvis or freeze-dried powder is resuspended with nutrient solution, by resuspended bacterium liquid: the envelope-bulk to weight ratio 1 of golden flower bacterium tea: (1～1000) is evenly sprayed onto on Camellia nitidissima, tealeaves after processing is placed in to 10-120 ℃, the aseptic or open condition bottom fermentation 1-90 days of relative humidity 20-90%; Described nutrient solution is one or more compositions of Fructus Rosae Laevigatae extract, compound amino acid, vitamin, trace element, glucose, wood sugar, fructose, sucrose, aseptic deionized water;
Step 4: use causes fragrant liquid the tealeaves of step 3 gained is fumigated to 0.1-90 hour under aseptic or open condition, after stifling, under 20-220 ℃ of condition, cure 2 seconds-48 hours, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak case or oak barrel that causes fragrant liquid generator, airtight placement obtains state aroma Camellia nitidissima after 1-300 days; It is described that to cause fragrant liquid be one or more compositions in fermentation, extraction thing in amino acid and reduced sugar of fermentation, extraction thing in wheat bran diffusion juice and/or beans enzymolysis liquid of Maotai producing mellow microorganism metabolite, Maotai producing mellow microorganism, Maotai producing mellow microorganism, Maotai, Fructus Rosae Laevigatae extract.
A kind of preparation method of golden flower bacterium bacterium liquid:
Get Fu brick tea or Pu'er tea and pulverize, by dull and stereotyped gradient dilution method, on isolation medium, in 20-40 ℃, cultivate until purifying; The golden flower bacterium colony of picking purifying, is seeded in fluid nutrient medium in 20-40 ℃ and cultivates 2-10 days, makes spore suspension concentration be controlled at 107-108/mL, obtains golden flower bacterium bacterium liquid;
Described slant medium is: tealeaves 1-100g/L, glossy ganoderma 1-100g/L, sucrose 10-100g/L, yeast extract 5-30g/L, sodium chloride 1-10g/L, agar 1-50g/L, remove agar and obtain fluid nutrient medium.
A kind of preparation method of Maotai producing mellow microorganism:
Getting the bent powder of 5-10 weight portion Maotai high-temperature daqu adds in the 90 weight portion sterilized waters or SPSS that bead is housed, on 50-300rpm shaking table in 20-60 ℃ of shaken cultivation 10-12 minute, after gradient dilution spread plate, in 20-60 ℃, cultivate 1-50 hour again, by colony inoculation, in fluid nutrient medium, the fragrant dominant bacteria of product sauce obtaining is bacterium; Slant medium is: glucose 1-100g/L, peptone 10-50g/L, yeast extract 1-50g/L, tealeaves 1-200g/L, glossy ganoderma 1-100g/L, sodium chloride 0.5-10g/L, agar 1-50g/L, removes agar and be fluid nutrient medium.
Embodiment
Embodiment 1
State aroma Camellia nitidissima by 1g become to sample tea, the loose capsule bacterium of 0.01g coronoid process, 0.01g Aspergillus form.
The preparation method of the loose capsule bacterium of coronoid process:
Get Fu brick tea and pulverize, by dull and stereotyped gradient dilution method, on isolation medium, in 20 ℃, cultivate until purifying; The loose capsule bacterium of the coronoid process of picking purifying, is seeded in fluid nutrient medium in 20 ℃ and cultivates 2 days, makes spore suspension concentration be controlled at 107/mL, obtains the loose capsule bacterium bacterium liquid of coronoid process;
Described slant medium is: tealeaves 10g/L, glossy ganoderma 30g/L, sucrose 10g/L, yeast extract 5g/L, sodium chloride 1g/L, agar 10g/L, remove agar and obtain fluid nutrient medium.
The preparation method of Aspergillus:
Getting the bent powder of 5g Maotai high-temperature daqu adds in the 90g sterilized water that bead is housed, on 50-300rpm shaking table in 20 ℃ of shaken cultivation 12 minutes, after gradient dilution spread plate, in 20 ℃, cultivate 50 hours again, from flat board, select the better Aspergillus colony inoculation of growth in fluid nutrient medium, the fragrant dominant bacteria of product sauce obtaining is bacterium; Slant medium is: glucose 1g/L, peptone 10g/L, yeast extract 10g/L, tealeaves 200g/L, glossy ganoderma 100g/L, sodium chloride 1g/L, agar 1g/L, remove agar and be fluid nutrient medium.
The preparation method of state aroma Camellia nitidissima:
Step 1: steam tealeaves with high-temperature steam and carry out sterilizing for 1 minute and make it softening, controlled humidity is 20% simultaneously; The ratio that the loose capsule bacterium bacterium liquid of coronoid process is added to 0.1mL according to every 100g tea sprays on tealeaves equably, and the tealeaves after fully mixing dries 1 minute in 40 ℃ in confined conditions; And then will use the resuspended bacterium liquid of the Aspergillus of nutrient solution to be evenly sprayed onto on tealeaves; Described nutrient solution is Fructus Rosae Laevigatae extract, compound amino acid, vitamin, trace element, glucose, wood sugar, sucrose, fructose, aseptic deionized water composition;
Step 2: by above-mentioned tealeaves at humidity 30%, 20 ℃ of temperature and aseptic condition bottom fermentation;
Step 3: use to cause fragrant liquid the fermented tea of gained is fumigated 0.1 hour under aseptic condition, after stifling, under 220 ℃ of conditions, cure 2 seconds hours, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak case that causes fragrant liquid generator, airtight ageing obtains state aroma golden flower bacterium fermented tea finished product after 1 day, can make tea cake through steam pressure; It is described that to cause fragrant liquid be that fermentation, extraction thing in amino acid and reduced sugar of Maotai producing mellow microorganism metabolite, Maotai producing mellow microorganism, Maotai, Fructus Rosae Laevigatae extract form.
Embodiment 2
A state aroma Camellia nitidissima, is characterized in that being comprised of 99.99g tealeaves, 10g golden flower bacterium, 10g Maotai producing mellow microorganism.
The preparation method of golden flower bacterium bacterium liquid::
Get Pu'er tea and pulverize, by dull and stereotyped gradient dilution method, on isolation medium, in 40 ℃, cultivate until purifying; The E.intermedius bacterium colony of picking purifying, thank a watt loose capsule bacterium bacterium colony, E.repens bacterium colony, be seeded in fluid nutrient medium in 40 ℃ and cultivate 10 days, make spore suspension concentration be controlled at 108/mL, obtain golden flower bacterium bacterium liquid;
Described slant medium is: tealeaves 100g/L, glossy ganoderma 1g/L, sucrose 100g/L, yeast extract 10g/L, sodium chloride 10g/L, agar 50g/L, remove agar and obtain fluid nutrient medium.
The preparation method of Maotai producing mellow microorganism:
Getting the bent powder of 10g Maotai high-temperature daqu adds in the 90g SPSS that bead is housed, on 300rpm shaking table in 60 ℃ of shaken cultivation 12 minutes, after gradient dilution spread plate, in 60 ℃, cultivate 50 hours again, bacillus subtilis, bacillus alcalophilus, cured shape bacillus bacterium colony are inoculated in respectively to fluid nutrient medium, and the fragrant dominant bacteria of product sauce obtaining is bacterium; Slant medium is: glucose 100g/L, peptone 50g/L, yeast extract 10g/L, tealeaves 200g/L, glossy ganoderma 1g/L, sodium chloride 10g/L, agar 10g/L, removes agar and be fluid nutrient medium.
The preparation method of a kind of state aroma Camellia nitidissima:
Step 1: the pass-through type that the fresh leaves of tea plant of harvesting is carried out 7 hours withers, presents after wilted condition in 250 ℃ fresh tea and completes 1 minute, carries out 50 minutes knead after cooling to obtain kneading leaf;
Step 2: sterilizing is after 1 minute under 121 ℃ of high steams under air-proof condition will to knead leaf, and the amount inoculation 0.1ml golden flower bacterium bacterium liquid by every 100g sterilizing tealeaves, in 20 ℃ of temperature, 50% time cultivation and fermentation of humidity 1 day, obtains golden flower bacterium tea;
Step 3: after the wet thallus of Maotai producing mellow microorganism is resuspended with nutrient solution, by resuspended bacterium liquid: the envelope-bulk to weight ratio of golden flower bacterium tea is evenly sprayed onto on Camellia nitidissima at 1: 1, the tealeaves after processing is placed in to 10 ℃, the aseptic condition bottom fermentation of relative humidity 20% 1 day; Described nutrient solution is Fructus Rosae Laevigatae extract, compound amino acid, vitamin, trace element, glucose, wood sugar, fructose, sucrose, aseptic deionized water composition;
Step 4: use to cause fragrant liquid the tealeaves of step 3 gained is fumigated 0.1 hour under aseptic condition, after stifling, under 220 ℃ of conditions, cure 2 seconds, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak barrel that causes fragrant liquid generator, airtight placement obtains state aroma Camellia nitidissima after 1 day; It is described that to cause fragrant liquid be that fermentation, extraction thing in wheat bran diffusion juice, beans enzymolysis liquid of Maotai producing mellow microorganism metabolite, Maotai producing mellow microorganism, Maotai producing mellow microorganism fermentation, extraction thing, Maotai, the Fructus Rosae Laevigatae extract in amino acid and reduced sugar forms.
Embodiment 3
A state aroma Camellia nitidissima, is characterized in that being comprised of 10g tealeaves, 0.1g golden flower bacterium, 3g Maotai producing mellow microorganism.
A kind of preparation method of golden flower bacterium bacterium liquid:
Get Fu brick tea and pulverize, by dull and stereotyped gradient dilution method, on isolation medium, in 25 ℃, cultivate until purifying; The coronoid process of picking purifying loose capsule bacterium, E.amstelodami, E.intermedius, thank a watt loose capsule bacterium, E.repens, Eurotium costiforme, the loose capsule bacterium of wax leaf, red loose capsule bacterium bacterium colony, be seeded in fluid nutrient medium and cultivate 4 days in 25 ℃, make spore suspension concentration be controlled at 108/mL, obtain golden flower bacterium bacterium liquid;
Described slant medium is: tealeaves 10g/L, glossy ganoderma 10g/L, sucrose 10g/L, yeast extract 5g/L, sodium chloride 1g/L, agar 10g/L, remove agar and obtain fluid nutrient medium.
A kind of preparation method of Maotai producing mellow microorganism:
Getting the bent powder of 6g Maotai high-temperature daqu adds in the 90g SPSS that bead is housed, on 100rpm shaking table in 25 ℃ of shaken cultivation 1 minute, after gradient dilution spread plate, in 30 ℃, cultivate 10 hours again, from Aspergillus, white mucor, rhizopus, pears head mould, false yeast, endomycopsis, Hansenula yeast, lactic acid bacteria, acetic acid bacteria, bacillus licheniformis, bacillus subtilis, bacillus alcalophilus, cured shape bacillus, bacillus pumilus, bacillus amyloliquefaciens, bafillus natto bacterium colony is inoculated in respectively fluid nutrient medium, the fragrant dominant bacteria of product sauce obtaining is bacterium, slant medium is: glucose 10g/L, peptone 10g/L, yeast extract 15g/L, tealeaves 100g/L, glossy ganoderma 50g/L, sodium chloride 1g/L, agar 10g/L, removes agar and be fluid nutrient medium.
The preparation method of a kind of state aroma Camellia nitidissima:
Step 1: steam tealeaves with high-temperature steam and carry out sterilizing for 3 minutes and make it softening, controlled humidity is 25% simultaneously; The ratio that golden flower bacterium bacterium liquid is added to 1mL according to every 100g tea sprays on tealeaves equably, and the tealeaves after fully mixing dries 10 minutes in 45 ℃ in confined conditions; And then will use the resuspended bacterium liquid of Maotai producing mellow microorganism of nutrient solution to be evenly sprayed onto on tealeaves; Described nutrient solution is Fructus Rosae Laevigatae extract, glucose, wood sugar, sucrose, fructose, aseptic deionized water composition;
Step 2: by above-mentioned tealeaves at humidity 40%, 25 ℃ of temperature and aseptic condition bottom fermentation;
Step 3: use to cause fragrant liquid the fermented tea of gained is fumigated 10 hours under open condition, after stifling, under 60 ℃ of conditions, cure 1 hour, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak barrel that causes fragrant liquid generator, airtight ageing obtains state aroma golden flower bacterium fermented tea finished product after 10 days, can make tea cake through steam pressure; It is described that to cause fragrant liquid be that fermentation, extraction thing in amino acid and reduced sugar of Maotai producing mellow microorganism, Maotai producing mellow microorganism, Fructus Rosae Laevigatae extract form.
Embodiment 4
A state aroma Camellia nitidissima, is characterized in that being comprised of 30g tealeaves, 8g golden flower bacterium, 1g Maotai producing mellow microorganism.
A kind of preparation method of golden flower bacterium bacterium liquid::
Get Pu'er tea and pulverize, by dull and stereotyped gradient dilution method, on isolation medium, in 30 ℃, cultivate until purifying; The loose capsule bacterium bacterium colony of the E.intermedius of picking purifying, wax leaf, is seeded in fluid nutrient medium in 30 ℃ and cultivates 8 days, makes spore suspension concentration be controlled at 108/mL, obtains golden flower bacterium bacterium liquid;
Described slant medium is: tealeaves 30g/L, glossy ganoderma 5g/L, sucrose 10g/L, yeast extract 3g/L, sodium chloride 6g/L, agar 45g/L, remove agar and obtain fluid nutrient medium.
A kind of preparation method of Maotai producing mellow microorganism:
Getting the bent powder of 9g Maotai high-temperature daqu adds in the 90g sterilized water that bead is housed, on 150rpm shaking table in 50 ℃ of shaken cultivation 11 minutes, after gradient dilution spread plate, in 30 ℃, cultivate 30 hours again, pears head mould, false yeast, endomycopsis, Hansenula yeast, lactic acid bacteria bacterium colony are inoculated in respectively to fluid nutrient medium, and the fragrant dominant bacteria of product sauce obtaining is bacterium; Slant medium is: glucose 30g/L, peptone 25g/L, yeast extract 10g/L, tealeaves 130g/L, glossy ganoderma 60g/L, sodium chloride 0.5g/L, agar 20g/L, removes agar and be fluid nutrient medium.
The preparation method of a kind of state aroma Camellia nitidissima:
Step 1: the pass-through type that the fresh leaves of tea plant of harvesting is carried out 4.5 hours withers, presents after wilted condition in 160 ℃ fresh tea and completes 16 minutes, carries out 18 minutes knead after cooling to obtain kneading leaf;
Step 2: sterilizing is after 15 minutes under 121 ℃ of high steams under air-proof condition will to knead leaf, and the amount inoculation 2ml golden flower bacterium bacterium liquid by every 100g sterilizing tealeaves, in 48 ℃ of temperature, 57% time cultivation and fermentation of humidity 4.5 days, obtains golden flower bacterium tea;
Step 3: after the wet thallus of Maotai producing mellow microorganism is resuspended with nutrient solution, by resuspended bacterium liquid: the envelope-bulk to weight ratio of golden flower bacterium tea is evenly sprayed onto Camellia nitidissima at 1: 1000, tealeaves after processing is placed in to 20 ℃, the open condition bottom fermentation of relative humidity 70% 10 days; Described nutrient solution is Fructus Rosae Laevigatae extract, compound amino acid, vitamin, fructose, sucrose, aseptic deionized water composition;
Step 4: use to cause fragrant liquid the tealeaves of step 3 gained is fumigated 30 hours under open condition, after stifling, under 120 ℃ of conditions, cure 2 hours, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak barrel that causes fragrant liquid generator, airtight placement obtains state aroma Camellia nitidissima after 160 days; It is described that to cause fragrant liquid be that the fermentation, extraction thing in amino acid and reduced sugar forms at wheat bran diffusion juice, Maotai producing mellow microorganism for Maotai producing mellow microorganism, Maotai producing mellow microorganism.
Embodiment 5
A state aroma Camellia nitidissima, is characterized in that being comprised of 90g tealeaves, 0.5g golden flower bacterium, 1g Maotai producing mellow microorganism.
A kind of preparation method of golden flower bacterium bacterium liquid:
Get Pu'er tea and pulverize, by dull and stereotyped gradient dilution method, on isolation medium, in 28 ℃, cultivate until purifying; The E.intermedius of picking purifying, thank a watt loose capsule bacterium, E.repens bacterium colony, be seeded in fluid nutrient medium in 40 ℃ and cultivate 3 days, make spore suspension concentration be controlled at 107/mL, obtain golden flower bacterium bacterium liquid;
Described slant medium is: tealeaves 30g/L, glossy ganoderma 90g/L, sucrose 10g/L, yeast extract 5g/L, sodium chloride 1g/L, agar 40g/L, remove agar and obtain fluid nutrient medium.
A kind of preparation method of Maotai producing mellow microorganism:
Getting the bent powder of 9.5g Maotai high-temperature daqu adds in the 90g sterilized water that bead is housed, on 280rpm shaking table in 50 ℃ of shaken cultivation 12 minutes, after gradient dilution spread plate, in 50 ℃, cultivate 40 hours again, white mucor, pears head mould, endomycopsis, Hansenula yeast, lactic acid bacteria, acetic acid bacteria, bacillus subtilis, bacillus alcalophilus bacterium colony are inoculated in respectively to fluid nutrient medium, and the fragrant dominant bacteria of product sauce obtaining is bacterium; Slant medium is: glucose 80g/L, peptone 15g/L, yeast extract 25g/L, tealeaves 90g/L, glossy ganoderma 30g/L, sodium chloride 1g/L, agar 50g/L, removes agar and be fluid nutrient medium.
The preparation method of a kind of state aroma Camellia nitidissima:
Step 1: steam tealeaves with high-temperature steam and carry out sterilizing for 9.5 minutes and make it softening, controlled humidity is 28% simultaneously; The ratio that golden flower bacterium bacterium liquid is added to 0.5mL according to every 100g tea sprays on tealeaves equably, and the tealeaves after fully mixing dries 19 minutes in 48 ℃ in confined conditions; And then will use the resuspended bacterium liquid of Maotai producing mellow microorganism of nutrient solution to be evenly sprayed onto on tealeaves; Described nutrient solution is Fructus Rosae Laevigatae extract, vitamin, trace element, glucose, sucrose, aseptic deionized water composition;
Step 2: by above-mentioned tealeaves at humidity 40%, 35 ℃ of temperature and aseptic condition bottom fermentation;
Step 3: use to cause fragrant liquid the fermented tea of gained is fumigated 10 hours under open condition, after stifling, under 150 ℃ of conditions, cure 24 hours, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak barrel that causes fragrant liquid generator, airtight ageing obtains state aroma golden flower bacterium fermented tea finished product after 150 days, can make tea cake through steam pressure; It is described that to cause fragrant liquid be that Maotai producing mellow microorganism, Maotai, Fructus Rosae Laevigatae extract form.
Embodiment 6
A state aroma Camellia nitidissima, is characterized in that being comprised of 55g tealeaves, 3g golden flower bacterium, 7g Maotai producing mellow microorganism.
A kind of preparation method of golden flower bacterium bacterium liquid::
Get Pu'er tea and pulverize, by dull and stereotyped gradient dilution method, on isolation medium, in 20-40 ℃, cultivate until purifying; The E.amstelodami of picking purifying, E.repens, Eurotium costiforme bacterium colony, be seeded in fluid nutrient medium in 38 ℃ and cultivate 7 days, makes spore suspension concentration be controlled at 107/mL, obtains golden flower bacterium bacterium liquid;
Described slant medium is: tealeaves 60g/L, glossy ganoderma 30g/L, sucrose 6g/L, yeast extract 20g/L, sodium chloride 2g/L, agar 45g/L, remove agar and obtain fluid nutrient medium.
A kind of preparation method of Maotai producing mellow microorganism:
Getting the bent powder of 9g Maotai high-temperature daqu adds in the 90g sterilized water that bead is housed, on 280rpm shaking table in 55 ℃ of shaken cultivation 10 minutes, after gradient dilution spread plate, in 35 ℃, cultivate 20 hours again, bacillus alcalophilus, cured shape bacillus, bacillus pumilus, bacillus amyloliquefaciens, white mucor, rhizopus bacterium colony are inoculated in respectively to fluid nutrient medium, and the fragrant dominant bacteria of product sauce obtaining is bacterium; Slant medium is: glucose 70g/L, peptone 45g/L, yeast extract 20g/L, tealeaves 80g/L, glossy ganoderma 60g/L, sodium chloride 3g/L, agar 35g/L, removes agar and be fluid nutrient medium.
The preparation method of a kind of state aroma Camellia nitidissima:
Step 1: the pass-through type that the fresh leaves of tea plant of harvesting is carried out 6.5 hours withers, presents after wilted condition in 200 ℃ fresh tea and completes 10 minutes, carries out 30 minutes knead after cooling to obtain kneading leaf;
Step 2: will knead leaf sterilizing after 25 minutes under 121 ℃ of high steams under air-proof condition, and by the amount inoculation 0.5ml golden flower bacterium bacterium liquid of every 100g sterilizing tealeaves, in temperature 45 C, 57% time cultivation and fermentation of humidity 3 days, obtain golden flower bacterium tea;
Step 3: after Maotai producing mellow microorganism pulvis is resuspended with nutrient solution, by resuspended bacterium liquid: the envelope-bulk to weight ratio of golden flower bacterium tea is evenly sprayed onto on Camellia nitidissima at 1: 100, the tealeaves after processing is placed in to 110 ℃, the aseptic condition bottom fermentation of relative humidity 70% 70 days; Described nutrient solution is vitamin, trace element, glucose, wood sugar, aseptic deionized water composition;
Step 4: use to cause fragrant liquid the tealeaves of step 3 gained is fumigated 30 hours under open condition, after stifling, under 60 ℃ of conditions, cure 8 hours, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak case that causes fragrant liquid generator, airtight placement obtains state aroma Camellia nitidissima after 250 days; It is described that to cause fragrant liquid be that the fermentation, extraction thing in amino acid and reduced sugar, Fructus Rosae Laevigatae extract form at wheat bran diffusion juice, Maotai producing mellow microorganism for Maotai producing mellow microorganism, Maotai producing mellow microorganism.
Embodiment 7
A state aroma Camellia nitidissima, is characterized in that being comprised of 50g tealeaves, 0.8g golden flower bacterium, 3g Maotai producing mellow microorganism.
A kind of preparation method of golden flower bacterium bacterium liquid:
Get Fu brick tea and pulverize, by dull and stereotyped gradient dilution method, on isolation medium, in 30 ℃, cultivate until purifying; The loose capsule bacterium of the Eurotium costiforme of picking purifying, wax leaf, red loose capsule bacterium, the loose capsule bacterium bacterium colony of coronoid process, be seeded in fluid nutrient medium in 38 ℃ and cultivate 8 days, makes spore suspension concentration be controlled at 108/mL, obtains golden flower bacterium bacterium liquid;
Described slant medium is: tealeaves 50g/L, glossy ganoderma 80g/L, sucrose 20g/L, yeast extract 18g/L, sodium chloride 1g/L, agar 25g/L, remove agar and obtain fluid nutrient medium.
A kind of preparation method of Maotai producing mellow microorganism:
Getting the bent powder of 6g Maotai high-temperature daqu adds in the 90g SPSS that bead is housed, on 100rpm shaking table in 30 ℃ of shaken cultivation 10 minutes, after gradient dilution spread plate, in 40 ℃, cultivate 35 hours again, Aspergillus, white mucor, bacillus subtilis, false yeast, bacillus pumilus bacterium colony are inoculated in respectively to fluid nutrient medium, and the fragrant dominant bacteria of product sauce obtaining is bacterium; Slant medium is: glucose 70g/L, peptone 18g/L, yeast extract 30g/L, tealeaves 170g/L, glossy ganoderma 30g/L, sodium chloride 6g/L, agar 20g/L, removes agar and be fluid nutrient medium.
The preparation method of a kind of state aroma Camellia nitidissima:
Step 1: steam tealeaves with high-temperature steam and carry out sterilizing for 3 minutes and make it softening, controlled humidity is 25% simultaneously; The ratio that golden flower bacterium bacterium liquid is added to 2mL according to every 100g tea sprays on tealeaves equably, and the tealeaves after fully mixing dries 80 minutes in 60 ℃ in confined conditions; And then will use the resuspended bacterium liquid of Maotai producing mellow microorganism of nutrient solution to be evenly sprayed onto on tealeaves; Described nutrient solution is Fructus Rosae Laevigatae extract, trace element, glucose, wood sugar, sucrose, aseptic deionized water composition;
Step 2: by above-mentioned tealeaves at humidity 80%, 35 ℃ of temperature and aseptic condition bottom fermentation;
Step 3: use to cause fragrant liquid the fermented tea of gained is fumigated 40 hours under open condition, after stifling, under 200 ℃ of conditions, cure 36 hours, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak barrel that causes fragrant liquid generator, airtight ageing obtains state aroma golden flower bacterium fermented tea finished product after 60 days, can make tea cake through steam pressure; It is described that to cause fragrant liquid be that the fermentation, extraction thing of Maotai producing mellow microorganism in amino acid and reduced sugar, Maotai, Fructus Rosae Laevigatae extract form.
Embodiment 8
A state aroma Camellia nitidissima, is characterized in that being comprised of 45g tealeaves, 3g golden flower bacterium, 6g Maotai producing mellow microorganism.
A kind of preparation method of golden flower bacterium bacterium liquid:
Get Pu'er tea and pulverize, by dull and stereotyped gradient dilution method, on isolation medium, in 28 ℃, cultivate until purifying; Picking purifying thank a watt loose capsule bacterium, E.repens, Eurotium costiforme bacterium colony, be seeded in fluid nutrient medium in 35 ℃ and cultivate 5.5 days, make spore suspension concentration be controlled at 108/mL, obtain golden flower bacterium bacterium liquid;
Described slant medium is: tealeaves 75g/L, glossy ganoderma 35g/L, sucrose 9g/L, yeast extract 0.8g/L, sodium chloride 2g/L, agar 40g/L, remove agar and obtain fluid nutrient medium.
A kind of preparation method of Maotai producing mellow microorganism:
Getting the bent powder of 7g Maotai high-temperature daqu adds in the 90g sterilized water that bead is housed, on 230rpm shaking table in 55 ℃ of shaken cultivation 11 minutes, after gradient dilution spread plate, in 54 ℃, cultivate 32 hours again, white mucor, endomycopsis, Hansenula yeast, acetic acid bacteria, bacillus licheniformis, bacillus subtilis, bacillus alcalophilus, cured shape bacillus, bacillus amyloliquefaciens bacterium colony are inoculated in respectively to fluid nutrient medium, and the fragrant dominant bacteria of product sauce obtaining is bacterium; Slant medium is: glucose 90g/L, peptone 7g/L, yeast extract 5.8g/L, tealeaves 130g/L, glossy ganoderma 30g/L, sodium chloride 5g/L, agar 20g/L, removes agar and be fluid nutrient medium.
The preparation method of a kind of state aroma Camellia nitidissima:
Step 1: the pass-through type that the fresh leaves of tea plant of harvesting is carried out 6.6 hours withers, presents after wilted condition in 150 ℃ fresh tea and completes 20 minutes, carries out 35 minutes knead after cooling to obtain kneading leaf;
Step 2: sterilizing is after 20 minutes under 121 ℃ of high steams under air-proof condition will to knead leaf, and the amount inoculation 3ml golden flower bacterium bacterium liquid by every 100g sterilizing tealeaves, in 28 ℃ of temperature, 60% time cultivation and fermentation of humidity 7 days, obtains golden flower bacterium tea;
Step 3: after Maotai producing mellow microorganism wet thallus is resuspended with nutrient solution, by resuspended bacterium liquid: the envelope-bulk to weight ratio of golden flower bacterium tea is evenly sprayed onto on Camellia nitidissima at 1: 10, the tealeaves after processing is placed in to 60 ℃, the aseptic condition bottom fermentation of relative humidity 70% 40 days; Described nutrient solution is Fructus Rosae Laevigatae extract, vitamin, trace element, glucose, sucrose, aseptic deionized water composition;
Step 4: use to cause fragrant liquid the tealeaves of step 3 gained is fumigated 30 hours under open condition, after stifling, under 90 ℃ of conditions, cure 16 hours, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak case that causes fragrant liquid generator, airtight placement obtains state aroma Camellia nitidissima after 60 days; It is described that to cause fragrant liquid be that fermentation, extraction thing in wheat bran diffusion juice, beans enzymolysis liquid of Maotai producing mellow microorganism, Maotai producing mellow microorganism, Maotai, Fructus Rosae Laevigatae extract form.
Embodiment 9
A state aroma Camellia nitidissima, is characterized in that being comprised of 45g tealeaves, 0.7g golden flower bacterium, 3.8g Maotai producing mellow microorganism.
A kind of preparation method of golden flower bacterium bacterium liquid:
Get Fu brick tea and pulverize, by dull and stereotyped gradient dilution method, on isolation medium, in 38 ℃, cultivate until purifying; The loose capsule bacterium of the coronoid process of picking purifying, thank a watt loose capsule bacterium, E.repens, Eurotium costiforme, red loose capsule bacterium bacterium colony, be seeded in fluid nutrient medium in 28 ℃ and cultivate 8.5 days, make spore suspension concentration be controlled at 108/mL, obtain golden flower bacterium bacterium liquid;
Described slant medium is: tealeaves 30g/L, glossy ganoderma 50g/L, sucrose 8g/L, yeast extract 4.5g/L, sodium chloride 2g/L, agar 3.5g/L, remove agar and obtain fluid nutrient medium.
A kind of preparation method of Maotai producing mellow microorganism:
Getting the bent powder of 9g Maotai high-temperature daqu adds in the 90g sterilized water that bead is housed, on 280rpm shaking table in 50 ℃ of shaken cultivation 10 minutes, after gradient dilution spread plate, in 35 ℃, cultivate 40 hours again, rhizopus, false yeast, endomycopsis, acetic acid bacteria, bacillus licheniformis, bacillus subtilis, bacillus alcalophilus, bacillus pumilus, bafillus natto bacterium colony are inoculated in respectively to fluid nutrient medium, and the fragrant dominant bacteria of product sauce obtaining is bacterium; Slant medium is: glucose 70g/L, peptone 35g/L, yeast extract 8g/L, tealeaves 60g/L, glossy ganoderma 70g/L, sodium chloride 8.5g/L, agar 25g/L, removes agar and be fluid nutrient medium.
The preparation method of a kind of state aroma Camellia nitidissima:
Step 1: steam tealeaves with high-temperature steam and carry out sterilizing for 7 minutes and make it softening, controlled humidity is 28% simultaneously; The ratio that golden flower bacterium bacterium liquid is added to 2.5mL according to every 100g tea sprays on tealeaves equably, and the tealeaves after fully mixing dries 60 minutes in 60 ℃ in confined conditions; And then will use the resuspended bacterium liquid of Maotai producing mellow microorganism of nutrient solution to be evenly sprayed onto on tealeaves; Described nutrient solution is Fructus Rosae Laevigatae extract, compound amino acid, glucose, wood sugar, sucrose, fructose, aseptic deionized water composition;
Step 2: by above-mentioned tealeaves at humidity 70%, 35 ℃ of temperature and aseptic condition bottom fermentation;
Step 3: use to cause fragrant liquid the fermented tea of gained is fumigated 55 hours under open condition, after stifling, under 180 ℃ of conditions, cure 40 hours, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak case that causes fragrant liquid generator, airtight ageing obtains state aroma golden flower bacterium fermented tea finished product after 180 days, can make tea cake through steam pressure; It is described that to cause fragrant liquid be that fermentation, extraction thing in amino acid and reduced sugar of Maotai producing mellow microorganism, Maotai producing mellow microorganism, Maotai form.
Embodiment 10
A state aroma Camellia nitidissima, is characterized in that being comprised of 56g tealeaves, 3.6g golden flower bacterium, 7.3g Maotai producing mellow microorganism.
A kind of preparation method of golden flower bacterium bacterium liquid:
Get Pu'er tea and pulverize, by dull and stereotyped gradient dilution method, on isolation medium, in 36 ℃, cultivate until purifying; Picking purifying thank a watt loose capsule bacterium, E.repens, Eurotium costiforme bacterium colony, be seeded in fluid nutrient medium in 36 ℃ and cultivate 9 days, make spore suspension concentration be controlled at 107/mL, obtain golden flower bacterium bacterium liquid;
Described slant medium is: tealeaves 60g/L, glossy ganoderma 34g/L, sucrose 10g/L, yeast extract 3g/L, sodium chloride 5g/L, agar 50g/L, remove agar and obtain fluid nutrient medium.
A kind of preparation method of Maotai producing mellow microorganism:
Getting the bent powder of 8g Maotai high-temperature daqu adds in the 90g sterilized water that bead is housed, on 250rpm shaking table in 45 ℃ of shaken cultivation 10-12 minute, after gradient dilution spread plate, in 45 ℃, cultivate 36 hours again, false yeast, endomycopsis, Hansenula yeast, bacillus licheniformis, bacillus subtilis, cured shape bacillus bacterium colony are inoculated in respectively to fluid nutrient medium, and the fragrant dominant bacteria of product sauce obtaining is bacterium; Slant medium is: glucose 78g/L, peptone 50g/L, yeast extract 30g/L, tealeaves 160g/L, glossy ganoderma 5g/L, sodium chloride 0.8g/L, agar 9g/L, removes agar and be fluid nutrient medium.
The preparation method of a kind of state aroma Camellia nitidissima:
Step 1: the pass-through type that the fresh leaves of tea plant of harvesting is carried out 6.5 hours withers, presents after wilted condition in 90 ℃ fresh tea and completes 5 minutes, carries out 20 minutes knead after cooling to obtain kneading leaf;
Step 2: sterilizing is after 20 minutes under 121 ℃ of high steams under air-proof condition will to knead leaf, and the amount inoculation 1ml golden flower bacterium bacterium liquid by every 100g sterilizing tealeaves, in 40 ℃ of temperature, 58% time cultivation and fermentation of humidity 7 days, obtains golden flower bacterium tea;
Step 3: after Maotai producing mellow microorganism freeze-dried powder is resuspended with nutrient solution, by resuspended bacterium liquid: the envelope-bulk to weight ratio of golden flower bacterium tea is evenly sprayed onto Camellia nitidissima at 1: 500, tealeaves after processing is placed in to 30 ℃, the aseptic condition bottom fermentation of relative humidity 75% 60 days; Described nutrient solution is Fructus Rosae Laevigatae extract, vitamin, trace element, fructose, sucrose, aseptic deionized water composition;
Step 4: use to cause fragrant liquid the tealeaves of step 3 gained is fumigated 70 hours under open condition, after stifling, under 80 ℃ of conditions, cure 16 hours, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak barrel that causes fragrant liquid generator, airtight placement obtains state aroma Camellia nitidissima after 20 days; Described cause fragrant liquid be Maotai producing mellow microorganism, Maotai producing mellow microorganism at wheat bran diffusion juice, Maotai producing mellow microorganism fermentation, extraction thing, Maotai, the composition in amino acid and reduced sugar.
Test example
Test example
Tea Polyphenols detection method:
1. reagent preparation: ferrous tartrate solution: take 1.0g ferrous sulfate (FeSO
47H
2o) and 5.0g sodium potassium tartrate tetrahydrate (C
14h
40
6kNa4H
20), water dissolves and is settled to 1L (low temperature preserve the term of validity 10 days).PH7.5 phosphate buffer: 1/15mol/L sodium hydrogen phosphate: take 23.9g disodium hydrogen phosphate (Na
2hPO
412H20), be settled to 1L after being dissolved in water; 1/15mol/L potassium dihydrogen phosphate: take the potassium dihydrogen phosphate (KH of drying 2 hours through 110 ℃
2pO
4) 9.08g, after being dissolved in water, be settled to 1L; Get the disodium phosphate soln 85mL of 1/15mol/L and the potassium dihydrogen phosphate 15mL of 1/15mol/L mixes.
2. concrete operations:
(ordinary tea leaves is soaked 3 times with boiling water accurately to draw test solution (being converted into identical tea amount), merge tea, during detection, get the tea of identical tea amount), the volumetric flask of injection 25mL, add water 4mL and ferrous tartrate solution 5mL, fully mix, then add pH7.5 phosphate buffer to scale, use 10mm cuvette, at wavelength 540nm place, measure absorbance (A), with blank reagent solution, make reference, measure absorbance A 2.Measure sample liquid that 25ml fully mixes in 50ml volumetric flask, add 95% ethanol 15ml, fully shake up, place 15min, water constant volume, Filter paper filtering.
Formula: Tea Polyphenols (mg/l)=(A-A2) * 1.975 * 2 * K * 1000/V;
K: extension rate;
III, total acid and ester detection method be with reference to GB/T10345-2007, result be total acid with acetic acid reach 3.0g/1, total ester reaches 2.60g/l (getting the liquid of identical tea amount) with ethyl acetate.
Tea polysaccharide detection method:
Extraction process: tealeaves → pulverizing → deionized water lixiviate → filtration → adjusting pH to 6.5 → 3 times 95% ethanol precipitation → centrifugal → essence is got precipitation → acetone, absolute ether, absolute ethyl alcohol and repeatedly alternately washed 3 times → vacuum drying → tea polysaccharide medicinal extract.
Assay:
The preparation of calibration curve: get concentration 0,0.025,0.05,0.1,0.15,0.2mg/ml glucose solution 1ml in tool plug test tube, ice bath, respectively add 0.05% By Anthrone Sulphuric acid reagent 4ml, mix, boiling water bath 7min, is chilled to rapidly room temperature with mobile water, place after 10min, in 620nm place, measure absorbance, the absorbance (A) of take is ordinate, and concentration of glucose (C) is made calibration curve and regression equation for abscissa.
The preparation of tea polysaccharide solution: by weight accurately taking the medicinal extract that is equivalent to 1g tealeaves, with deionized water dissolving, be settled in 100ml volumetric flask, shake up, standby.
Tea polysaccharide assay: get above-mentioned tea polysaccharide solution 1ml in tool plug test tube, measure absorbance (A), concentration of glucose (C) in regression equation calculation tea polysaccharide extract by assay method.According to the content of tea polysaccharide in following formula calculation sample.
Tea polysaccharide content (%)=(C * D)/(W * 100)
In formula, C is concentration of glucose in tea polysaccharide extract (mg/ml), the extension rate that D is tea polysaccharide, and W is the quality (g) of tea polysaccharide medicinal extract.
The Tea Polyphenols and the total acid and ester content results that detect as stated above embodiment 1-9 are as follows:
As seen from the above table, the state aroma Camellia nitidissima of gained of the present invention has not only preserved Tea Polyphenols, the tea polysaccharide in former tea, has preserved tea fragrant, also contain the fragrant acid and the ester that possess of requiring of Maotai, so it has possessed tea perfume (or spice) and aroma simultaneously.
Claims (8)
1.Yi Zhong state aroma Camellia nitidissima, is characterized in that by 1-99.99 weight portion tealeaves, 0.01-10 weight portion golden flower bacterium, 0.01-10 weight portion Maotai producing mellow microorganism or produces the fragrant function bacterium of sauce forming.
2. a kind of state according to claim 1 aroma Camellia nitidissima, is characterized in that described tealeaves is that fresh tea or one-tenth are sampled tea.
3. a kind of state according to claim 1 aroma Camellia nitidissima, is characterized in that described Maotai producing mellow microorganism is for producing one or more compositions in the Aspergillus obtaining with separation and purification Daqu, white mucor, rhizopus, pears head mould, false yeast, endomycopsis, Hansenula yeast, lactic acid bacteria, acetic acid bacteria, bacillus licheniformis, bacillus subtilis, bacillus alcalophilus, cured shape bacillus, bacillus pumilus, bacillus amyloliquefaciens, bafillus natto from Maotai.
4. a kind of state according to claim 1 aroma Camellia nitidissima, it is characterized in that described golden flower bacterium for coronoid process loose capsule bacterium, E.amstelodami, E.intermedius that from Pu'er tea or Fu brick tea, separation and purification obtains, thank to one or more in a watt loose capsule bacterium, E.repens, Eurotium costiforme, the loose capsule bacterium of wax leaf, red loose capsule bacterium.
5. a kind of state according to claim 1 aroma Camellia nitidissima, is characterized in that being prepared by following methods:
Step 1: steam tealeaves with high-temperature steam and carry out sterilizing for 1-10 minute and make it softening, controlled humidity is 20-30% simultaneously; The ratio of under aseptic condition, golden flower bacterium bacterium liquid being added to 0.1-5mL according to every 100g tea sprays on tealeaves equably, and the tealeaves after fully mixing dries 1-90 minute in 40-70 ℃ in confined conditions; And then will use the resuspended bacterium liquid of the Maotai producing mellow microorganism of nutrient solution to be evenly sprayed onto on tealeaves; Described nutrient solution is one or more compositions of Fructus Rosae Laevigatae extract, compound amino acid, vitamin, trace element, glucose, wood sugar, sucrose, fructose, aseptic deionized water;
Step 2: by above-mentioned tealeaves at humidity 30-90%, temperature 20-40 ℃ and aseptic condition bottom fermentation;
Step 3: use causes fragrant liquid the fermented tea of gained is fumigated to 0.1-90 hour under aseptic or open condition, after stifling, under 20-220 ℃ of condition, cure 2 seconds-48 hours, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak case or oak barrel that causes fragrant liquid generator, after airtight ageing 1-300 days, obtain state aroma golden flower bacterium fermented tea finished product, can make tea cake through steam pressure; It is described that to cause fragrant liquid be Maotai producing mellow microorganism or produce one or more compositions in fermentation, extraction thing in amino acid and reduced sugar of the fragrant function bacterium metabolite of sauce, Maotai producing mellow microorganism, Maotai, Fructus Rosae Laevigatae extract.
6. a kind of state according to claim 1 aroma Camellia nitidissima, is characterized in that being prepared by following methods:
Step 1: the pass-through type that the fresh leaves of tea plant of harvesting is carried out 3-7 hour withers, presents after wilted condition in the 60-250 ℃ of 1-30 minute that completes fresh tea, carries out 10-50 minute knead after cooling to obtain kneading leaf;
Step 2: will knead leaf under air-proof condition under 121 ℃ of high steams after sterilizing 1-30 minute, under aseptic condition, by the amount of every 100g sterilizing tealeaves, inoculate 0.1-5ml golden flower bacterium bacterium liquid, cultivation and fermentation 1-10 days under temperature 20-50 ℃, humidity 50-70%, obtains golden flower bacterium tea;
Step 3: after wet thallus, pulvis or the freeze-dried powder of Maotai producing mellow microorganism or the fragrant function bacterium of product sauce is resuspended with nutrient solution, by resuspended bacterium liquid: the envelope-bulk to weight ratio 1 of golden flower bacterium tea: (1～1000) is evenly sprayed onto on Camellia nitidissima, tealeaves after processing is placed in to 10-120 ℃, the aseptic or open condition bottom fermentation 1-90 days of relative humidity 20-90%; Described nutrient solution is one or more compositions of Fructus Rosae Laevigatae extract, compound amino acid, vitamin, trace element, glucose, wood sugar, fructose, sucrose, aseptic deionized water;
Step 4: use causes fragrant liquid the tealeaves of step 3 gained is fumigated to 0.1-90 hour under aseptic or open condition, after stifling, under 20-220 ℃ of condition, cure 2 seconds-48 hours, in the middle of after curing, tealeaves being packed into while hot, be provided with in the oak case or oak barrel that causes fragrant liquid generator, airtight placement obtains state aroma Camellia nitidissima after 1-300 days; It is described that to cause fragrant liquid be Maotai producing mellow microorganism or produce one or more compositions in fermentation, extraction thing in amino acid and reduced sugar of fermentation, extraction thing in wheat bran diffusion juice and/or beans enzymolysis liquid of the fragrant function bacterium metabolite of sauce, Maotai producing mellow microorganism, Maotai producing mellow microorganism, Maotai, Fructus Rosae Laevigatae extract.
7. a kind of state according to claim 1 aroma Camellia nitidissima, is characterized in that golden flower bacterium bacterium liquid prepared by following methods:
Get Fu brick tea or Pu'er tea and pulverize, by dull and stereotyped gradient dilution method, on isolation medium, in 20-40 ℃, cultivate until purifying; The golden flower bacterium colony of picking purifying, is seeded in fluid nutrient medium in 20-40 ℃ and cultivates 2-10 days, makes spore suspension concentration be controlled at 107-108/mL, obtains golden flower bacterium bacterium liquid;
Described slant medium is: tealeaves 1-100g/L, glossy ganoderma 1-100g/L, sucrose 10-100g/L, yeast extract 5-30g/L, sodium chloride 1-10g/L, agar 1-50g/L, remove agar and obtain fluid nutrient medium.
8. a kind of state according to claim 1 aroma Camellia nitidissima, is characterized in that Maotai producing mellow microorganism prepared by following methods:
Getting the bent powder of 5-10 weight portion Maotai high-temperature daqu adds in the 90 weight portion sterilized waters or SPSS that bead is housed, on 50-300rpm shaking table in 20-60 ℃ of shaken cultivation 10-12 minute, after gradient dilution spread plate, in 20-60 ℃, cultivate 1-50 hour again, different bacterium colonies are inoculated in respectively to fluid nutrient medium, and the fragrant dominant bacteria of product sauce obtaining is bacterium; Slant medium is: glucose 1-100g/L, peptone 10-50g/L, yeast extract 1-50g/L, tealeaves 1-200g/L, glossy ganoderma 1-100g/L, sodium chloride 0.1-10g/L, agar 1-50g/L, removes agar and be fluid nutrient medium.
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