CN104473247A - Chinese bouquet dendrobe sour soup and preparation method thereof - Google Patents
Chinese bouquet dendrobe sour soup and preparation method thereof Download PDFInfo
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- CN104473247A CN104473247A CN201410658220.3A CN201410658220A CN104473247A CN 104473247 A CN104473247 A CN 104473247A CN 201410658220 A CN201410658220 A CN 201410658220A CN 104473247 A CN104473247 A CN 104473247A
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- JAJWGJBVLPIOOH-IZYKLYLVSA-M sodium taurocholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 JAJWGJBVLPIOOH-IZYKLYLVSA-M 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000011003 system suitability test Methods 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L23/00—Soups; Sauces; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention belongs to the technical field of health foods and particularly relates to a Chinese bouquet dendrobe sour soup and a preparation method of the Chinese bouquet dendrobe sour soup. The Chinese bouquet dendrobe sour soup is prepared from the following substances in part by weight: 1-90 parts of dendrobe, 1-90 parts of fructus rosae laevigatae and/or pyracantha fortuneana and 0.01-90 parts of combined starter culture. The Chinese bouquet dendrobe sour soup has an immunity enhancing function through pharmacology experiments, and the Chinese bouquet dendrobe sour soup has higher content of active ingredient-dendrobium polysaccharides through content detection and analysis.
Description
Technical field
The invention belongs to food technology field, be specifically related to a kind of state aroma stem of noble dendrobium acid soup composition.
Background technology
Immunity is the defense mechanism of human body self, is human bioequivalence and any foreign matter (virus, bacterium etc.) eliminating external intrusion; Process is old and feeble, damage, the ability of mutant cell and virus infected cell in the own cells of dead, sex change and identification and handling body.Immunology Today is thought, immunity is the physiological reaction of human bioequivalence and eliminating " dissident ".What perform this function in human body is immune system.People stand much pressure in daily life.Continuing to be exposed to external pressure is considered to cause human body to reduce the immunity being no matter the disease that microorganism or other reasons cause.The health of hypoimmunity very easily causes the infection such as bacterium, virus, fungi, and therefore hypoimmunity the most directly shows is exactly liable to illness.People continue the problem that " natural " scheme of seeking solves raising immunity for many years.
The stem of noble dendrobium (formal name used at school: Dendrobium nobile Lindl), has another name called lofty or bottomless palpus, bracketplant, Lin Lan, golden hairpin China etc.Medicinal plant, has certain antipyretic effect, can promote gastric secretion, aid digestion; There are the effects such as enhancing is metabolic, anti-ageing, reduction blood fat.
Summary of the invention
For these reasons, the present inventor is on the basis studied for many years, and obtain a kind of state aroma stem of noble dendrobium acid soup composition, pharmacological evaluation shows, the present composition, has good immunity humidification.
The present invention is achieved through the following technical solutions.
A kind of state aroma stem of noble dendrobium acid soup composition, containing the 1-90 weight portion stem of noble dendrobium, the 1-90 weight portion fruit of Cherokee rose and/or fiery sour jujube, 0.01-90 weight portion composite ferment in described composition.
The described stem of noble dendrobium is one or more in the fresh stem of noble dendrobium, dendrobe ultrafine powder, dendrobe tissue culture seedling, stem of noble dendrobium endophyte, stem of noble dendrobium endophyte tunning, fermentation dendrobe powder.
Described composite ferment is Maotai producing mellow microorganism, the fragrant zymotic fluid of sauce, seedling man acid soup, plant composite enzyme, produce in sour bacillus, saccharomycete, lactic acid bacteria, product GABA lactic acid bacteria, the fragrant yeast of sauce, amino acid, carbohydrate, salt, Kefir grain one or more.
Described amino acid be glutamic acid, alanine, proline, tryptophan, methionine, valine, histidine, lysine, serine, aspartic acid, threonine, leucine, isoleucine, phenylalanine,
essenceone or more in propylhomoserin, citrulling, GABA.
Described Maotai producing mellow microorganism be screen from the high-temperature daqu of Maotai production obtain bacillus licheniformis, bacillus subtilis, bacillus amyloliquefaciens one or more.
Described seedling man acid soup be Miao ethnic group the acid of hair rice, tomato is sour, shrimp is sour, fish are sour, meat is sour, the acid of dish class, the acid of pickles sour, white rosy clouds, poor peppery acid, one in hydrochloric acid.
Prepare a method for described composition, comprise the following steps:
Step 1: by the stem of noble dendrobium, the fruit of Cherokee rose and/or the mixing of fiery sour jujube, add its weight 1 ~ 3 times of water and carry out fragmentation, add the enzyme preparation of gross weight 0.001 ~ 5% after adding water, the ascorbic acid of 0.01 ~ 5% and/or phytic acid again, under 30 ~ 65 DEG C of conditions, process 1 ~ 5 hour, obtained enzymolysis feed liquid; Described enzyme preparation is one or more in cellulase, pectase, amylase, Lysozyme, hemicellulase, carbohydrase, protease;
Step 2: in above-mentioned enzymolysis feed liquid, adds its weight 0.01 ~ 10% composite ferment, after mixing, in 30 ~ 65 DEG C of condition bottom fermentations 1 ~ 180 hour, obtains state's aroma zymotic fluid;
Step 3: in above-mentioned state aroma zymotic fluid, add the product GABA lactic acid bacteria of its weight 0.01 ~ 10% or produce sour bacillus, mixing, in 30 ~ 45 DEG C of condition bottom fermentations 5 ~ 80 hours, take out fermentation liquid, filter, Ji get state aroma stem of noble dendrobium acid soup composition.
Embodiment
Below by way of the description of detailed description of the invention, the invention will be further described, but this is not limitation of the present invention, those skilled in the art are according to basic thought of the present invention, various amendment or improvement can be made, but only otherwise depart from basic thought of the present invention, all within the scope of the present invention.
The stem of noble dendrobium, the fruit of Cherokee rose, fiery sour jujube are all purchased from Mao Wei bio tech ltd, Guizhou.
The test of pesticide effectiveness 1: improve immunity test
The immunology effect of the activation evaluation group compound of macrophage is used in vitro in cell culture test
Sample:
The stem of noble dendrobium: it is for subsequent use that the commercially available fresh stem of noble dendrobium makes dendrobe ultrafine powder after drying;
The fruit of Cherokee rose: it is for subsequent use that the commercially available fresh fruit of Cherokee rose makes fruit of Cherokee rose Ultramicro-powder after drying.
Fire sour jujube: it is for subsequent use that commercially available fresh fiery sour jujube makes fiery sour jujube Ultramicro-powder after drying.
Method:
A. cell chulture
Raw264.7 clone is maintained continuously in the DMEM with 10%FCS (hyclone).Their every 2-3 days go down to posterity once, cultivate in the atmosphere of 5% carbon dioxide at 37 DEG C.
B. Macrophage inflammatory protein-1α
Apply Raw264.7 cell on the cover slip with the density of 105 cells/cover glass, and make it adhere to.Cell is cultivated 2 hours with the combination of different material.After cultivation, with 106 latex bead/105 cells (cell: pearl=1: 10) cultivate cell 2 hours.Cell on air-dry cover glass, and use methyl alcohol fixed cell.Cell dyes 10 minutes through Giemsa, and uses microscopic counting.
The result of Macrophage inflammatory protein-1α is expressed as phagocytosis index, that is, the intracellular sum of every 100 cells.
The material of concentration shown in use table 1 carries out testing (often testing 4 samples).The data of average phagocytosis index are also summarized in table 1 together with standard deviation.The data of Using statistics methods analyst show that in t inspection, confidence level is greater than 95%.
Table 1 phagocytosis results contrast
Described fermenting agent 1 is Maotai producing mellow microorganism; Described fermenting agent 2 is the fragrant yeast of sauce.
Note: be used alone the stem of noble dendrobium and compare, * P < 0.05, * * P < 0.01.
The data of table 1 show, the phagocytosis index of the independent application stem of noble dendrobium, fiery sour jujube, fruit of Cherokee rose generation certain numerical value, but, the combination of the stem of noble dendrobium and fiery sour jujube and/or the fruit of Cherokee rose provide than the obtainable obviously higher phagocytosis index (P < 0.05 or P < 0.01) of above-mentioned substance; And after adding fermenting agent, there is better immunoenhancement result, and after using different fermenting agents, there is close effect.
Therefore, the invention provides composition by mutually acting synergistically with the immunity benefit providing enhancing.
The test of pesticide effectiveness 2: reducing blood lipid is tested
1, healthy adult male rat, at the end of the laundering period, body weight 200 ± 20g, first-selected SD rat, only often organizes 8-12.
2, model feed
20.0% sucrose, 15% lard, 1.2% cholesterol, 0.2% sodium taurocholate is added, appropriate casein, calcium monohydrogen phosphate, stone flour etc. in basal feed.Except crude fat, the moisture of model feed, crude protein, crude fat, crude fibre, coarse ash, calcium, phosphorus, calcium: phosphorus all will reach the national standard maintaining feed.
3, dosage grouping and given the test agent give the time
It is 30 days that given the test agent gives the time.
Table 1 experimental animal group designs
4, experimental procedure
4.1 laundering period: rat feeding maintains feed and observes 5-7 days under barrier system.
4.2 modeling phases
Be divided into 2 groups at random by body weight, 10 rats give to maintain feed as blank group, and 40 give model feed as model control group.Weigh in weekly 1 time.
After model control group gives model feed 1-2 week, blank group and model control group rat non-fasting blood sampling (the intraocular corner of the eyes or afterbody), separation of serum as early as possible after blood sampling, measures serum TC, TG, LDL-C, HDL-C level.According to TC level, model control group is divided into 4 groups at random, blank group and the model control group afterwards of dividing into groups compares TC, TG, LDL-C, HDL-C difference, and there are no significant.
3.3 given the test agent give
After grouping, three trial drug group (group 3,4,5) warp-mouths every day give given the test agent, blank group and model control group give the coordinative solvent of same volume simultaneously, blank group continues to give to maintain feed, model control group and three trial drug groups continue to give model feed, and regularly weigh in, non-fasting blood sampling at the end of experiment, separation of serum as early as possible after blood sampling, measures serum TC, TG, LDL-C, HDL-C level.
4, observation index: TC, TG, LDL-C, HDL-C.
5, data processing and result judge
Table 2 this product is on the impact (X+SD) of high fat diet lipid of mice level
Note:
Δrepresent significant difference compared with Normal group,
Δ Δrepresent that difference is extremely remarkable;
*represent significant difference compared with high fat control group, * * represents that difference is extremely remarkable.
Result of the test shows, product of the present invention can reduce Induced by High Fat Diet in Rats serum TG, TC and LDL-c level effectively, simultaneously significantly raises HDL-C level, thus effectively suppresses the rising of rat fat, and comparatively TC is obvious to the reducing effect of TG level.
Test 3:
In order to control product quality of the present invention better, guaranteeing safe and effective for medication, adopting high performance liquid chromatography to carry out assay, for the quality control of product of the present invention to the active ingredient dendrobium polysaccharide of the main ingredient stem of noble dendrobium in product of the present invention.
1, instrument and reagent
1.1 instrument Waters alliance high performance liquid chromatographs, 2996 PDADs and Empower work station (Wa ters company of the U.S.), CP225D 100,000/electronic balance (German sartorius company), Milli-Q ultrapure water machine (MILLI-PORE company of the U.S.), KQ-500E type ultrasonic cleaner (Kunshan Ultrasonic Instruments Co., Ltd.).
1.2 reagent dendrobium polysaccharide reference substances (purchased from Nat'l Pharmaceutical & Biological Products Control Institute), state's aroma stem of noble dendrobium acid soup composition is (by method preparation described in embodiment 3, lot number is respectively 140306,140309,140312,140315,140319), methyl alcohol is chromatographically pure, and it is pure that all the other reagent are analysis.
2 methods and result
The preparation precision of 2.1 reference substance solution takes through P
2o
5the dendrobium polysaccharide reference substance of drying under reduced pressure 24h is appropriate, and add methyl alcohol and dissolve, making concentration is 0.160mgmL
-1dendrobium polysaccharide solution is product storing solution in contrast.
The preparation precision of 2.2 need testing solutions measures state aroma stem of noble dendrobium acid soup composition 2.0mL in 25mL volumetric flask, and with 50% methanol dilution to scale, shake up, with 0.22 μm of filtering with microporous membrane, filtrate is as need testing solution.
The preparation of 2.3 negative control solutions is got not containing other medicinal material of the stem of noble dendrobium, according to legal system below 2.1.2 item for negative control solution by prescription.
2.4 chromatographic conditions and system suitability test chromatographic column are Akasil C18 post (4.6 × 250mm, 5 μm), mobile phase is methyl alcohol-1% glacial acetic acid aqueous solution (1: 9), flow velocity institute 1.0mL/min, column temperature: 30 DEG C, determined wavelength: 281nm.Get reference substance solution respectively, need testing solution and each 10.0 μ L injection liquid chromatographies of negative control solution, theoretical cam curve calculates should be not less than 2000 by dendrobium polysaccharide.
2.5 calibration curves preparations respectively accurate draw reference substance storing solution 0.5,1.0,2.0,4.0,5.0mL, put in 5mL measuring bottle, with methanol dilution to scale, shake up, in contrast product solution.The each 10.0 μ L of the above-mentioned reference substance solution of accurate absorption respectively, injection liquid chromatography, measures peak area.Take peak area as ordinate, with reference substance concentration for abscissa does linear regression, obtaining regression equation is Y=123764X+74.235, r=0.9998.Result shows, the linear relationship of dendrobium polysaccharide within the scope of 0.144 ~ 1.44 μ g is good.
2.5.1 precision test precision draws reference substance solution 10.0 μ L, and continuous sample introduction 6 times, the RSD of its peak area average is 1.68%.
2.5.2 same need testing solution is got in stability test, respectively 0,2,4,8,12,24h sample introduction 10. μ L, the RSD of its peak area average is 0.59%.
2.5.3 replica test gets same batch sample, and according to 2.2 parallel preparations of lower method, 6 parts of need testing solutions, sample introduction 10 μ L respectively, the RSD of its peak area average is 1.40%.
2.5.4 average recovery test is got with a collection of national wine fragrant stem of noble dendrobium acid soup composition (lot number 140312) 1.0mL in 25mL measuring bottle, totally 6 parts, adds dendrobium polysaccharide reference substance solution (0.144mgmL respectively
-1) 4.0mL,
Be prepared into need testing solution according to method below 2.1.2 item, sample introduction 10.0 μ L, calculate the rate of recovery, to obtain average recovery rate be 100.3%, RSD is 0.83%, the results are shown in Table 3.
Table 1 dendrobium polysaccharide assay recovery test result (n=6)
2.5.5 state's aroma stem of noble dendrobium acid soup composition totally 5 batches and the dendrobe ultrafine powder prepared described in assay Example 4-8, need testing solution is prepared into according to method below 2.1.2 item, accurate absorption reference substance solution and need testing solution 10.0 μ L respectively, injection liquid chromatography, measure peak area, calculate content with external standard method, the results are shown in Table 4.
Table 4 sample size measurement result
| Lot number | Dendrobium polysaccharide content/(mg/mL) | RSD/％(n＝3) |
| Embodiment 4 | 0.5594 | 1.47 |
| Embodiment 5 | 0.5451 | 1.38 |
| Embodiment 6 | 0.5523 | 1.56 |
| Embodiment 7 | 0.5343 | 1.69 |
| Embodiment 8 | 0.5552 | 1.51 |
| Dendrobe ultrafine powder | 0.3456 | 1.48 |
Result of the test shows, product of the present invention can improve the level of dendrobium polysaccharide in composition effectively.
Embodiment
Embodiment 1
Step 1: by fresh for the 1g stem of noble dendrobium, the 1g fruit of Cherokee rose is mixed pulverize after mix, add its 2g water and carry out fragmentation, then add add water after the enzyme preparation of gross weight 0.001%, the ascorbic acid of 0.01%, under 30 DEG C of conditions, process 1 hour, obtained enzymolysis feed liquid; Described enzyme preparation consists of: weight ratio 30% cellulase, 35% pectase, 15% amylase, 20% Lysozyme;
Step 2: in above-mentioned enzymolysis feed liquid, adds the acid of its weight 0.01% tomato, after mixing, in 30 DEG C of condition bottom fermentations 1 hour, obtains state's aroma zymotic fluid;
Step 3: in above-mentioned state aroma zymotic fluid, adds the product GABA lactic acid bacteria of its weight 0.01%, mixes, in 30 DEG C of condition bottom fermentations 5 hours, take out fermentation liquid, filter, the filtrate of gained concentrated through cryogenic vacuum, granulation postlyophilization makes state's aroma stem of noble dendrobium acid powder.
Embodiment 2
Step 1: by 10g dendrobe ultrafine powder, the mixing of 20g fire sour jujube, add its weight 1.5 times of water and carry out fragmentation, then add add water after the enzyme preparation of gross weight 0.05%, the phytic acid of 0.1%, under 35 DEG C of conditions, process 1.5 hours, obtained enzymolysis feed liquid; Described enzyme preparation consists of: weight ratio 30% cellulase, 10% amylase, 10% Lysozyme, 45% carbohydrase, 5% protease;
Step 2: in above-mentioned enzymolysis feed liquid, add its weight 6.5% amino acid (comprising 1% alanine, 0.5% proline, 0.4% tryptophan, 1% methionine, 0.8% valine, 1.2% histidine, 0.6% lysine, 0.7% serine, 0.3% aspartic acid), after mixing, in 45 DEG C of condition bottom fermentations 18 hours, obtain state's aroma zymotic fluid;
Step 3: in above-mentioned state aroma zymotic fluid, adds the product GABA lactic acid bacteria of its weight 0.5%, mixes, in 35 DEG C of condition bottom fermentations 20 hours, take out fermentation liquid, filter, the filtrate of gained concentrated through cryogenic vacuum, granulation postlyophilization makes state's aroma stem of noble dendrobium acid powder.
Embodiment 3
Step 1: by 90g stem of noble dendrobium endophyte, the 10g fruit of Cherokee rose and the mixing of 25g fire sour jujube, add its weight 2 times of water and carry out fragmentation, then add add water after the enzyme preparation of gross weight 0.5%, the ascorbic acid of 2%, under 55 DEG C of conditions, process 5 hours, obtained enzymolysis feed liquid; Described enzyme preparation consists of: weight ratio is 35% cellulase, 5% pectase, 25% amylase, 10% Lysozyme, 15% hemicellulase, 5% carbohydrase, 5% protease;
Step 2: in above-mentioned enzymolysis feed liquid, adds its weight 2% seedling man acid soup, after mixing, in 40 DEG C of condition bottom fermentations 60 hours, obtains state's aroma zymotic fluid;
Step 3: in above-mentioned state aroma zymotic fluid, adds the product GABA lactic acid bacteria of its weight 5%, mixes, in 30 DEG C of condition bottom fermentations 35 hours, take out fermentation liquid, filter, the filtrate of gained concentrated through cryogenic vacuum, after granulation, spraying dry makes stem of noble dendrobium acid powder.
Embodiment 4
Step 1: by 30g stem of noble dendrobium endophyte tunning, the mixing of the 25g fruit of Cherokee rose, add its weight 3 times of water and carry out fragmentation, add the enzyme preparation of gross weight 5% after adding water, the ascorbic acid of 0.1% and 3% phytic acid again, under 30 DEG C of conditions, process 1 hour, obtained enzymolysis feed liquid; Described enzyme preparation consists of: weight ratio 55% pectase, 15% Lysozyme, 18% hemicellulase, 12% carbohydrase;
Step 2: in above-mentioned enzymolysis feed liquid, adds its weight 8% and produces sour bacillus, 2% saccharomycete, after mixing, in 65 DEG C of condition bottom fermentations 180 hours, obtains state's aroma zymotic fluid;
Step 3: in above-mentioned state aroma zymotic fluid, adds the product acid bacillus of its weight 7%, mixes, in 30 DEG C of condition bottom fermentations 50 hours, take out fermentation liquid, filter, 100ml filtrate and 50mg xylitol, 10ml malic acid are made stem of noble dendrobium sour-soup drink after blending sterilizing.
Embodiment 5
Step 1: dendrobe powder of being fermented by 40g, the 5g fruit of Cherokee rose and the mixing of 8g fire sour jujube, add its weight 2 times of water and carry out fragmentation, then add add water after the enzyme preparation of gross weight 4.5%, the ascorbic acid of 5%, under 55 DEG C of conditions, process 3 hours, obtained enzymolysis feed liquid; Described enzyme preparation consists of: weight ratio 16% pectase, 28% amylase, 31% Lysozyme, 17% hemicellulase, 4% carbohydrase, 4% protease;
Step 2: in above-mentioned enzymolysis feed liquid, adds the fragrant zymotic fluid of its weight 9% sauce, after mixing, in 65 DEG C of condition bottom fermentations 90 hours, obtains state's aroma zymotic fluid;
Step 3: in above-mentioned state aroma zymotic fluid, adds the product GABA lactic acid bacteria of its weight 6%, mixes, in 40 DEG C of condition bottom fermentations 50 hours, take out fermentation liquid, filter, 100ml filtrate and 65mg maltose, 5ml acetic acid are made state's aroma stem of noble dendrobium sour-soup drink after blending sterilizing.
Embodiment 6
Step 1: by 5g dendrobe tissue culture seedling, the 10g fruit of Cherokee rose, the mixing of 10g fire sour jujube, add its weight 1 times of water and carry out fragmentation, add the enzyme preparation of gross weight 0.5% after adding water, the ascorbic acid of 0.1% and 1.5% phytic acid again, under 45 DEG C of conditions, process 3 hours, obtained enzymolysis feed liquid; Described enzyme preparation consists of: weight ratio is 22% cellulase, 5% amylase, 17% Lysozyme, 38% hemicellulase, 18% protease;
Step 2: in above-mentioned enzymolysis feed liquid, adds its weight 5% Kefir grain, after mixing, in 45 DEG C of condition bottom fermentations 160 hours, obtains state's aroma zymotic fluid;
Step 3: in above-mentioned state aroma zymotic fluid, adds the product GABA lactic acid bacteria of its weight 3%, mixes, in 38 DEG C of condition bottom fermentations 10 hours, take out fermentation liquid, filter, the filtrate of gained concentrated through cryogenic vacuum, granulation postlyophilization makes state's aroma stem of noble dendrobium acid powder.
Embodiment 7
Step 1: by 40g dendrobe ultrafine powder, the mixing of the 30g fruit of Cherokee rose, add its weight 2.6 times of water and carry out fragmentation, then add add water after the enzyme preparation of gross weight 3%, the phytic acid of 1.8%, under 35 DEG C of conditions, process 4.8 hours, obtained enzymolysis feed liquid; Described enzyme preparation consists of: weight ratio 11% cellulase, 6% pectase, 27% Lysozyme, 11% hemicellulase, 35% carbohydrase, 10% protease;
Step 2: in above-mentioned enzymolysis feed liquid, adds its weight 3.7% Maotai producing mellow microorganism, 3.7% and produces the salt of sour bacillus 2%, after mixing, in 35 DEG C of condition bottom fermentations 60 hours, obtains state's aroma zymotic fluid;
Step 3: in above-mentioned state aroma zymotic fluid, adds the product GABA lactic acid bacteria of its weight 7%, mixes, in 40 DEG C of condition bottom fermentations 20 hours, take out fermentation liquid, filter, the filtrate of gained concentrated through cryogenic vacuum, after granulation, spraying dry makes state's aroma stem of noble dendrobium acid powder.
Embodiment 8
Step 1: by fresh for the 70g stem of noble dendrobium, the mixing of the 20g fruit of Cherokee rose, add its weight 1.3 times of water and carry out fragmentation, then add add water after the enzyme preparation of gross weight 2.8%, the ascorbic acid of 2.5%, under 55 DEG C of conditions, process 4 hours, obtained enzymolysis feed liquid; Described enzyme preparation consists of: weight ratio 6% pectase, 28% amylase, 4% Lysozyme, 26% hemicellulase, 36% carbohydrase;
Step 2: in above-mentioned enzymolysis feed liquid, add its weight 2% seedling man acid soup, 1.5% plant composite enzyme, after mixing, in 60 DEG C of condition bottom fermentations 80 hours, obtain state's aroma zymotic fluid;
Step 3: in above-mentioned state aroma zymotic fluid, adds the product acid bacillus of its weight 5%, mixes, in 30 DEG C of condition bottom fermentations 30 hours, take out fermentation liquid, filter, the filtrate of gained concentrated through cryogenic vacuum, granulation postlyophilization makes state's aroma stem of noble dendrobium acid powder.
Embodiment 9
Step 1: by fresh for the 20g stem of noble dendrobium, the 30g fruit of Cherokee rose and the mixing of 18g fire sour jujube, add its weight 2 times of water and carry out fragmentation, add again the enzyme preparation of gross weight 1% after adding water, the ascorbic acid of 0.5% and 3.5% phytic acid, under 55 DEG C of conditions, process 4.5 hours, obtained enzymolysis feed liquid; Described enzyme preparation consists of: weight ratio 35% cellulase, 25% Lysozyme, 10% hemicellulase, 15% carbohydrase, 15% protease;
Step 2: in above-mentioned enzymolysis feed liquid, adds the fragrant yeast of its weight 10% sauce, after mixing, in 40 DEG C of condition bottom fermentations 150 hours, obtains state's aroma zymotic fluid;
Step 3: in above-mentioned state aroma zymotic fluid, adds the product acid bacillus of its weight 1.8%, mixes, in 40 DEG C of condition bottom fermentations 60 hours, take out fermentation liquid, filter, the filtrate of gained concentrated through cryogenic vacuum, granulation postlyophilization makes state's aroma stem of noble dendrobium acid powder.
Embodiment 10
Step 1: by 60g dendrobe ultrafine powder, the mixing of the 30g fruit of Cherokee rose, add its weight 1.8 times of water and carry out fragmentation, then add add water after the enzyme preparation of gross weight 1.5%, the phytic acid of 2.5%, under 60 DEG C of conditions, process 3.5 hours, obtained enzymolysis feed liquid; Described enzyme preparation consists of: weight ratio 30% cellulase, 20% pectase, 15% amylase, 25% Lysozyme, 10% carbohydrase;
Step 2: in above-mentioned enzymolysis feed liquid, adds its weight 5% Maotai producing mellow microorganism, after mixing, in 50 DEG C of condition bottom fermentations 100 hours, obtains state's aroma zymotic fluid;
Step 3: in above-mentioned state aroma zymotic fluid, add the product acid bacillus of its weight 7%, mix, in 35 DEG C of condition bottom fermentations 48 hours, take out fermentation liquid, filter, 100ml filtrate and 30mg xylitol, 8ml citric acid, 5ml acetic acid are made state's aroma stem of noble dendrobium sour-soup drink after blending sterilizing.
Claims (7)
1. state's aroma stem of noble dendrobium acid soup composition, is characterized in that, containing the 1-90 weight portion stem of noble dendrobium, the 1-90 weight portion fruit of Cherokee rose and/or fiery sour jujube, 0.01-90 weight portion composite ferment in described composition.
2. composition according to claim 1, is characterized in that, the described stem of noble dendrobium is one or more in the fresh stem of noble dendrobium, dendrobe ultrafine powder, dendrobe tissue culture seedling, stem of noble dendrobium endophyte, stem of noble dendrobium endophyte tunning, fermentation dendrobe powder.
3. composition according to claim 1, it is characterized in that, described composite ferment is Maotai producing mellow microorganism, the fragrant zymotic fluid of sauce, seedling man acid soup, plant composite enzyme, produce in sour bacillus, saccharomycete, lactic acid bacteria, product GABA lactic acid bacteria, the fragrant yeast of sauce, amino acid, carbohydrate, salt, Kefir grain one or more.
4. composition according to claim 3, it is characterized in that, described amino acid is one or more in glutamic acid, alanine, proline, tryptophan, methionine, valine, histidine, lysine, serine, aspartic acid, threonine, leucine, isoleucine, phenylalanine, arginine, citrulling, GABA.
5. composite ferment according to claim 3, is characterized in that, described Maotai producing mellow microorganism be screen from the high-temperature daqu of Maotai production obtain bacillus licheniformis, bacillus subtilis, bacillus amyloliquefaciens one or more.
6. composite ferment according to claim 3, is characterized in that, described seedling man acid soup be Miao ethnic group the acid of hair rice, tomato is sour, shrimp is sour, fish are sour, meat is sour, the acid of dish class, the acid of pickles sour, white rosy clouds, poor peppery acid, one in hydrochloric acid.
7. prepare a method for composition according to claim 1, it is characterized in that, comprise the following steps:
Step 1: by the stem of noble dendrobium, the fruit of Cherokee rose and/or the mixing of fiery sour jujube, add its weight 1 ~ 3 times of water and carry out fragmentation, add the enzyme preparation of gross weight 0.001 ~ 5% after adding water, the ascorbic acid of 0.01 ~ 5% and/or phytic acid again, under 30 ~ 65 DEG C of conditions, process 1 ~ 5 hour, obtained enzymolysis feed liquid; Described enzyme preparation is one or more in cellulase, pectase, amylase, Lysozyme, hemicellulase, carbohydrase, protease;
Step 2: in above-mentioned enzymolysis feed liquid, adds its weight 0.01 ~ 10% composite ferment, after mixing, in 30 ~ 65 DEG C of condition bottom fermentations 1 ~ 180 hour, obtains state's aroma zymotic fluid;
Step 3: in above-mentioned state aroma zymotic fluid, add the product GABA lactic acid bacteria of its weight 0.01 ~ 10% or produce sour bacillus, mixing, in 30 ~ 45 DEG C of condition bottom fermentations 5 ~ 80 hours, take out fermentation liquid, filter, Ji get state aroma stem of noble dendrobium acid soup composition.
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| CN107495039A (en) * | 2017-09-04 | 2017-12-22 | 安徽汉泰生物科技投资有限公司 | A kind of preparation method of the stem of noble dendrobium drink rich in r aminobutyric acids |
| CN112574841A (en) * | 2020-12-28 | 2021-03-30 | 黄大川 | Dendrobium officinale black wine and preparation method thereof |
| CN113893310A (en) * | 2020-07-06 | 2022-01-07 | 安琪纽特股份有限公司 | Mountain plant fermentation composition and preparation method and application thereof |
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| CN103704387A (en) * | 2013-12-26 | 2014-04-09 | 郭景龙 | Bouquet tea |
| CN103783226A (en) * | 2014-02-19 | 2014-05-14 | 郭景龙 | Soy sauce flavor type tea |
| CN103918813A (en) * | 2014-05-08 | 2014-07-16 | 郭景龙 | National bouquet golden camellia tea and method for preparing national bouquet golden camellia tea |
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| CN103704387A (en) * | 2013-12-26 | 2014-04-09 | 郭景龙 | Bouquet tea |
| CN103783226A (en) * | 2014-02-19 | 2014-05-14 | 郭景龙 | Soy sauce flavor type tea |
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| CN107495039A (en) * | 2017-09-04 | 2017-12-22 | 安徽汉泰生物科技投资有限公司 | A kind of preparation method of the stem of noble dendrobium drink rich in r aminobutyric acids |
| CN113893310A (en) * | 2020-07-06 | 2022-01-07 | 安琪纽特股份有限公司 | Mountain plant fermentation composition and preparation method and application thereof |
| CN112574841A (en) * | 2020-12-28 | 2021-03-30 | 黄大川 | Dendrobium officinale black wine and preparation method thereof |
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