CN106146111A - A kind of microorganism phosphate fertilizer and application thereof - Google Patents
A kind of microorganism phosphate fertilizer and application thereof Download PDFInfo
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- CN106146111A CN106146111A CN201510126501.9A CN201510126501A CN106146111A CN 106146111 A CN106146111 A CN 106146111A CN 201510126501 A CN201510126501 A CN 201510126501A CN 106146111 A CN106146111 A CN 106146111A
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Abstract
The present invention relates to agricultural fertilizer, a kind of microorganism phosphate fertilizer and application thereof.Microorganism phosphate fertilizer by weight percentage, the carrier of 50% 60% absorption bacterial strains, 30% 40% MAP and 10% calcium superphosphate;The bacterial strain of described carrier adsorption is that in bacillus subtilis, nitrogen-fixing bacteria, root nodule bacteria and silicate bacteria, any three kinds of bacterial strains are adsorbed in carrier respectively, then presses the mass fraction mixing of 1:1:1;Wherein, every gram of microorganism phosphate fertilizer is containing 1.0 1.2 × 108Individual bacterium cell.Use microorganism phosphate fertilizer of the present invention can make crop root prosperity healthy growth, volume increase, be green ecological, the indispensable fertilizer of green organic food simultaneously.
Description
Technical field
The present invention relates to agricultural fertilizer, a kind of microorganism phosphate fertilizer and application thereof.
Background technology
Plantation family is in order to ensure increases in grain production demand, and plantation family excess applies phosphate fertilizer and inorganic nutrients, makes
Unavailable fertilizer residual in soil is become too much, to cause heavy metal severe overweight.Phosphate anion in phosphate fertilizer
The cation such as calcium, magnesium is combined formation acid phosphate hard to tolerate in soil, and a large amount of harmful substances, pesticide are residual
Stay and control the growth nutrient of root system of plant, the absorption of moisture, undergrowth, cause crop failure,
Quality degradation, not only wastes phosphate fertilizer but also destroy soil aggregate, causes soil compaction, with much money
Belong to exceeds standard, and causes human body osteoporosis, has a strong impact on human body healthy.Agriculturally planting
Plant family and be continuously increased phosphorus and inorganic nutrients usage amount, can not produce high-quality crop, make greenhouse crops from
-1000 jin of yield in 500 jin of annual every mu of ground of yield in 2 years not etc. do not drop to booth salination, total crop failure.
Along with environment, safety food are increasingly paid attention to by China, the requirement of the tip of the tongue engineering is proposed, and then
Need to provide one that crop can be made to increase production further, stable yields, anti-continuous cropping, resistant to lodging, drought resisting, waterlogging-resistant,
Disease resistance, and deeper into solving the microorganism phosphate fertilizer of soil compaction.
Summary of the invention
It is an object of the invention to provide a kind of microorganism phosphate fertilizer and application thereof.
For achieving the above object, the technical solution used in the present invention is:
A kind of microorganism phosphate fertilizer, by weight percentage, 50%-60% adsorbs the load of bacterial strain to microorganism phosphate fertilizer
Body, 30%-40% MAP and 10% calcium superphosphate;The bacterial strain of described carrier adsorption is bacillus subtilis
In bacterium, nitrogen-fixing bacteria, root nodule bacteria and silicate bacteria, any three kinds of bacterial strains are adsorbed in carrier, then respectively
It is the ratio mixing of 1:1:1 in mass ratio;Wherein, every gram of microorganism phosphate fertilizer is containing 1.0-1.2 × 108Individual
Bacterium cell.
On described carrier, the adsorbance of various bacterial strains is identical;Root nodule bacteria are Semen sojae atricolor or peanut rhizobium.
It is 1 that the described bacterial strain rear bearing body of absorption respectively is blended in dissolved oxygen amount by the parts by weight of 1:1:1:
The ventilation of 0.5, at 30-40 DEG C concussion cultivate, until culture is tasteless.
Described carrier is straw, humic acids or farm manure.
Further, described straw is after saccharomyces cerevisiae is fermented, stand-by after pulverizing and sieving;
Humic acids was for pulverizing 80 mesh sieves;Farm manure is to ferment through saccharomyces cerevisiae, pulverizes and sieves after fermentation,
Decompose through Rhodopseudomonas spheroides again, stand-by.
The described saccharomyces cerevisiae inoculum concentration being inoculated on straw is 0.5%;The described farm manure of being inoculated in
Saccharomyces cerevisiae inoculum concentration is 0.5%, presses the inoculum concentration inoculation Rhodopseudomonas spheroides of 5% after fermentation again.
The application of a kind of microorganism phosphate fertilizer, applies plant growth other nutritious fertilizer required, then by described
Microorganism phosphate fertilizer is manured into soil substrate, then long-term cropping;Wherein, every mu of soil matrix applies
5-10kg microorganism phosphate fertilizer.
Or, described microorganism phosphate fertilizer is mixed with various crop seeds, in the substrate that is then manured into soil also
It is equipped with plant growth other nutritious fertilizer required, every mu of soil matrix applies 5-10kg microorganism phosphate fertilizer.
And then minimizing Phosphorus fertilizer usage, save phosphate rock resource 50-65% for country.
The crop of described plantation is the industrial crops such as field crop, vegetable, flowers, plants and trees, medical material, fruit tree.
Described crop other nutritious fertilizer required that applies is micro organism composite fertilizer;Wherein, microorganism is combined
Fertile preparation sees the record of Application No. ZL200910219977.1, prepares.
Wherein, bacterial strain is respectively as follows:
Bacillus subtilis (Bacillus subtilis): starch 7g, potassium dihydrogen phosphate 1g, sucrose
1g, yeast extract 1g, peptone 0.2g, soybean cake powder 4g, ammonium sulfate 1.5g, sodium chloride 1.5g, jade
Rice flour 0.2g, PH7.0-7.2.
Nitrogen-fixing bacteria (Azotobacter chrooccum Beijerinck): potassium dihydrogen phosphate 1g, CaCO32g、
Ferric chloride 0.1g, magnesium sulfate 0.2g, Nac10.2g, sodium molybdate 0.005g, sucrose 10g are dissolved in
Water, PH7.0.
Semen sojae atricolor or peanut rhizobium: sucrose 6g, starch 8g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.2g,
Calcium carbonate 1g, sodium chloride 0.2g, yeast extract 0.4g, PH6.8-7.0.
Silicate bacteria (Silicate bacteria): sucrose 10g, Nacl 10g, dipotassium hydrogen phosphate
2g, magnesium sulfate 0.5g, ammonium sulfate 1g, CaCO33g is soluble in water, PH=7.2.
Saccharomyces cerevisiae (Saccharomyces cerevisiae): 100g Semen Glycines Germinatus is added 1000ml
Water boils 30 minutes, take 100ml bean sprout juice and add 4g yeast extract, 15g sucrose 2gNacl, 1g
Peptone, PH=6.5-6.7.
Rhodopseudomonas spheroides (Rps.globiformis): 1g ammonium sulfate, 2gNacl, 0.5g phosphoric acid
Hydrogen dipotassium, 0.2g magnesium sulfate, 0.05g ferric chloride, 0.05mg copper sulfate, 0.05mg magnesium sulfate,
1mg zinc sulfate, 2g peptone, 0.1g yeast extract, PH=7.5, illumination 30-35 DEG C.
Advantage for present invention:
1. improve yield, improving quality, microorganism phosphate fertilizer of the present invention is applied to big Tanaka and coordinates crop
Required compound fertilizer uses, it is possible to make crop yield 5-20%, can increase fruit-setting rate simultaneously, and minimizing falls
Really, making completely filled fruit, ripe 3-10 days in advance, effect of increasing production was obvious, improving quality outward appearance, mouthfeel,
Color and luster, quality improves 1-2 grade, is the phosphate fertilizer of green ecological organic food first-selection simultaneously, extends
Product freshness date 7-15 days.
2. improve phosphorus utilization, reduce Phosphorus fertilizer usage, for country save phosphate rock resource 50-65% this
After microorganism in bright phosphate fertilizer enters soil, the amount reproduction of active microorganism bacterial strain can be fixed in air
Nitrogen, nitrogen in soil;Decompose the Organic residues in soil and the unavailable phosphorus of crop and ore deposit
Material, converts the available nutrient of crop, is greatly improved the utilization rate of fertilizer, makes cost reduce.
3. improvement soil, the amount reproduction of the microorganism fungus kind in microorganism phosphate fertilizer of the present invention, greatly
Improve the microecosystem of soil, promote the formation of soil aggregate, strengthen the breathability of soil,
Improve the water conservation fertilizer-preserving ability of profound soil, applying inorganic fertilizer in a large number causes soil plate throughout the year in releasing
Knot, improves soil activation.
Detailed description of the invention
Embodiment 1
A kind of microorganism phosphate fertilizer, microorganism phosphate fertilizer by weight percentage, the carrier of 50% absorption bacterial strain,
40% MAP and the mixing of 10% calcium superphosphate, pelletize in the usual way after mixing, comminutor import temperature
Spending 60 DEG C, outlet temperature 40 DEG C~50 DEG C, oven drying at low temperature, to moisture content 5~8%, sieves 80 mesh, both
Obtain microorganism phosphate fertilizer.The bacterial strain of described carrier adsorption is bacillus subtilis, nitrogen-fixing bacteria and peanut nodule
Bacterium, carrier is straw;Every gram of microorganism phosphate fertilizer is containing 1.0 × 108Individual bacterium cell.Wherein, MAP
Middle nitrogen containing 11%, phosphorus is containing 40%;Calcium superphosphate, containing effective P2O514%.
The carrier of absorption bacterial strain is that bacillus subtilis, nitrogen-fixing bacteria and peanut rhizobium are adsorbed in straw respectively
On stalk, on carrier, the adsorbance of various bacterial strains is identical, is the ratio mixing of 1:1:1 the most in mass ratio,
Dissolved oxygen amount be the ventilation of 1:0.5, at 30-40 DEG C concussion cultivate, until culture is tasteless.
Described carrier straw is that by the inoculum concentration of 0.5%, saccharomyces cerevisiae is inoculated in straw, lower of room temperature
Ferment, pulverized 80 mesh sieves after fermentation, stand-by.
Above-mentioned bacterial strains rejuvenation process respectively is:
The cultivation of bacillus subtilis (Bacillus subtilis): the preparation of culture medium: starch 7g,
Potassium dihydrogen phosphate 1g, sucrose 1g, yeast extract 1g, peptone 0.2g, soybean cake powder 4g, ammonium sulfate 1.5g,
Sodium chloride 1.5g, Semen Maydis powder 0.2g, PH7.0-7.2, in shaking table 30 DEG C/200 turns after sterilizing, cooling
Cultivate 20 hours, stand-by.
The cultivation of blown-ball Azotobacter (Azotobacter chrooccum Beijerinck): culture medium
Preparation: potassium dihydrogen phosphate 1g, CaCO32g, ferric chloride 0.1g, magnesium sulfate 0.2g, Nac10.2g,
Sodium molybdate 0.005g, sucrose 10g are dissolved in water, PH7.0, are inoculated in shaking table 30 DEG C after sterilizing, cooling
Cultivate 48 hours for/200 turns, stand-by.
Semen sojae atricolor or the cultivation of peanut rhizobium: the preparation of culture medium: sucrose 6g, starch 8g, phosphoric acid hydrogen
Dipotassium 0.5g, magnesium sulfate 0.2g, calcium carbonate 1g, sodium chloride 0.2g, yeast extract 0.4g, PH6.8-7.0,
Sterilizing, cultivates 20 hours in shaking table 30 DEG C/200 turns after cooling, stand-by.
The cultivation of silicate bacteria (Silicate bacteria): the preparation of culture medium: sucrose 10g,
Nacl 10g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.5g, ammonium sulfate 1g, CaCO33g is soluble in water,
PH=7.2 enters triangular flask 121 DEG C, sterilizing in 30 minutes, inoculates, 28 DEG C/200 turns trainings of upper shaking table after cooling
Support 48 hours, stand-by.
The preparation of saccharomyces cerevisiae (Saccharomyces cerevisiae) culture medium: 100g is yellow
Bean sprout adds in 1000ml water boils 30 minutes, takes 100ml bean sprout juice and adds 4g yeast extract, 15g
Sucrose 2gNacl, 1g peptone, PH=6.5-6.7, sterilizing 121 DEG C, inoculate after cooling in 30 minutes,
And cultivate 16-20 hour shaking table 28 DEG C/200 turns, stand-by.
The preparation of Rhodopseudomonas spheroides (Rps.globiformis) culture medium: 1g ammonium sulfate, 2gNacl,
0.5g dipotassium hydrogen phosphate, 0.2g magnesium sulfate, 0.05g ferric chloride, 0.05mg copper sulfate, 0.05mg
Magnesium sulfate, 1mg zinc sulfate, 2g peptone, 0.1g yeast extract, PH=7.5 soluble in water, sterilizing 115 DEG C,
Within 15 minutes, being cooled to 30 DEG C of inoculations, illumination 30-35 DEG C, 72-120 hour to peony bacterium solution.Above
Individual bacteria culture fluid all culture fluid with 1000ml are stand-by.
Embodiment 2
A kind of microorganism phosphate fertilizer, microorganism phosphate fertilizer by weight percentage, the carrier of 60% absorption bacterial strain,
30% MAP and the mixing of 10% calcium superphosphate, pelletize in the usual way after mixing, comminutor import temperature
Spending 60 DEG C, outlet temperature 40 DEG C~50 DEG C, oven drying at low temperature, to moisture content 5~8%, sieves 80 mesh, both
Obtain microorganism phosphate fertilizer.The bacterial strain of described carrier adsorption is silicate bacteria, nitrogen-fixing bacteria and rihizobium japonicum,
Carrier is humic acids;Every gram of microorganism phosphate fertilizer is containing 1.2 × 108Individual bacterium cell.Wherein, in MAP
Nitrogen containing 11%, phosphorus is containing 40%;Calcium superphosphate, containing effective P2O514%.
The carrier of absorption bacterial strain is that silicate bacteria, nitrogen-fixing bacteria and rihizobium japonicum are adsorbed in straw respectively
On, on carrier, the adsorbance of various bacterial strains is identical, is the ratio mixing of 1:1:1 the most in mass ratio,
Dissolved oxygen amount be the ventilation of 1:0.5, at 30-40 DEG C concussion cultivate, until culture is tasteless.
Described carrier humic acids is to pulverize 80 mesh sieves, stand-by.
Embodiment 3
A kind of microorganism phosphate fertilizer, microorganism phosphate fertilizer by weight percentage, the carrier of 55% absorption bacterial strain,
35% MAP and the mixing of 10% calcium superphosphate, pelletize in the usual way after mixing, comminutor import temperature
Spending 60 DEG C, outlet temperature 40 DEG C~50 DEG C, oven drying at low temperature, to moisture content 5~8%, sieves 80 mesh, both
Obtain microorganism phosphate fertilizer.The bacterial strain of described carrier adsorption is that bacillus subtilis, nitrogen-fixing bacteria and silicate are thin
Bacterium, carrier is farm manure;Every gram of microorganism phosphate fertilizer is containing 1.1 × 108Individual bacterium cell.Wherein, phosphoric acid one
In ammonium nitrogen containing 11%, phosphorus is containing 40%;Calcium superphosphate, containing effective P2O514%.
The carrier of absorption bacterial strain is that bacillus subtilis, nitrogen-fixing bacteria and silicate bacteria are adsorbed in straw respectively
On stalk, on carrier, the adsorbance of various bacterial strains is identical, is the ratio mixing of 1:1:1 the most in mass ratio,
Dissolved oxygen amount be the ventilation of 1:0.5, at 30-40 DEG C concussion cultivate, until culture is tasteless.
Described farm manure is that by the inoculum concentration of 0.5%, saccharomyces cerevisiae is inoculated in farm manure, lower of room temperature
Ferment, carries out decomposing through fermentation by saccharomyces cerevisiae at the inoculum concentration inoculation Rhodopseudomonas spheroides by 5% after fermentation
Farm manure, then pulverized 80 mesh sieves, stand-by.
Application examples
Executing kind of crop is field crop and vegetable industrial crops;Field crop is Semen Maydis, and vegetable economy is made
Thing is Fructus Fragariae Ananssae;Following mu is (667) in terms of little mu.
Matched group is
CK1For conventional disposable compound fertilizer, wherein: N18:P18:K18, total nutrient 54%, 50kg/
Mu;
CK2For conventional compound fertilizer, wherein: N15:P15:K15, total nutrient 45%, 50kg/ mu;
CK3For conventional Chemical Mixed Fertilizer, wherein: N15:P15:K15, total nutrient 45%, 50kg/ mu;
Above-mentioned matched group CK1、CK2And CK3When using, every mu of soil need the routine chasing after 15~20kg to use
The phosphoric acid diamidogen that mouth is fertile;CK simultaneously2And CK3Every mu of soil chases after the carbamide of 10~25kg again;
Experiment contrast group is:
Experiment contrast group is at above-mentioned matched group CK1、CK2And CK3When using, every mu of soil need mouth fertile
Embodiment of the present invention microorganism phosphate fertilizer (the i.e. SCK of 5-10kg1、SCK2And SCK3);SCK simultaneously2With
SCK3Every mu of soil chases after the carbamide of 15~20kg again;
Experimental group is:
SK is disposable micro organism composite fertilizer, wherein: N18:P6:K8 total nutrient 32%, 50kg/ mu;
The preparation of disposable micro organism composite fertilizer sees the record of Application No. ZL200910219977.1, preparation
Obtain.
Every mu of soil needs to chase after when above-mentioned disposable micro organism composite fertilizer is used the embodiment of the present invention of 5kg
Microorganism phosphate fertilizer.
The fertilizer of above-mentioned each matched group, experiment contrast group and experimental group is applied 666.7m2Field crop
Or in the soil matrix of vegetable industrial crops, then long-term cropping, then in process of crop growth, chase after phosphorus
Fertilizer and carbamide.Execute in seed bottom during fertilising, sidepiece have the isolation soil layer of 5-8cm with crop seed,
Or use the seeding machinery separated with seed by fertilizer to carry out ferti-seeding, the yield of crop and P
Utilization (seeing table 1 and 2).
Table 1
Corn yield | The utilization of P | |
CK1 | 1200-1400 | 33 |
CK2 | 1200-1400 | 35 |
CK3 | 1200-1400 | 35 |
SCK1 | 1400-1550 | 20 |
SCK2 | 1400-1500 | 19 |
SCK3 | 1400-1500 | 19 |
SK | 1450-1800 | 8 |
Table 2
Yield of Strawberry (ton) | Crop quality (sugar part) | The utilization of P | |
CK1 | 2-3.5 | 9 | 38 |
CK2 | 2-3.5 | 9 | 35-40 |
CK3 | 2-3.5 | 9 | 35-40 |
SCK1 | 3.5-4 | 11 | 19.8 |
SCK2 | 3.5-4 | 11.5 | 18.6 |
SCK3 | 3.5-4 | 11.5 | 18.6 |
SK | 4-5 | 13 | 9.6 |
By the above-mentioned visible SCK using microorganism phosphate fertilizer of the present invention of table 1 and 21、SCK2、SCK3And SK
Group, Seedling root is flourishing, and volume increase is substantially, according to there being drought resisting, resistant to lodging, solves field crop continuous cropping
Problem.Simultaneously according to the mode of above-mentioned experimental group, to field crop such as Semen Maydis continuous administration 3 years (or
Continuous three batches), industrial crops continuous administration 3~5 batches (including booth) such as vegetable, then detect soil
Substrate recovers ecosystem soil, will thoroughly solve field crop and industrial crops heavy metals exceeding standard, medicine
Residual, solution soil compaction, salination make crop thoroughly reach green ecological safety food standard.
And improve phosphorus utilization, reduce Phosphorus fertilizer usage, save phosphate rock resource 50-65% originally for country
After microorganism in invention phosphate fertilizer enters soil, the amount reproduction of active microorganism bacterial strain can fix air
In nitrogen, nitrogen in soil;Decompose the Organic residues in soil and the unavailable phosphorus of crop and
Mineral, converts the available nutrient of crop, is greatly improved the utilization rate of fertilizer, makes cost reduce.
It addition, use the special microorganism foliage fertilizer of experimental group of the present invention and long-term cropping, (foliage fertilizer is purchased
From in Sheng De source, Liaoning microorganism fertilizer industry Co., Ltd) it is used in combination, chronic administration can control soil
Passing disease and leaf, stem pathogen, the microbial bacteria contained by microorganism phosphate fertilizer combines organic and inorganic balance
Nutrient, create good growing environment to crop, make the full growth course of crop obtains reasonable micro-life
The protection of thing bacterium, makes crop reach the benefit of environmental protection, increasing both production and income, solves safety food difficult,
The problem that cost is high.
Claims (7)
1. a microorganism phosphate fertilizer, it is characterised in that: microorganism phosphate fertilizer by weight percentage, 50%-60%
The absorption carrier of bacterial strain, 30%-40% MAP and 10% calcium superphosphate;The bacterial strain of described carrier adsorption
Adsorb respectively for three kinds of bacterial strains any in bacillus subtilis, nitrogen-fixing bacteria, root nodule bacteria and silicate bacteria
In carrier, then press the mass fraction mixing of 1:1:1;Wherein, every gram of microorganism phosphate fertilizer is containing 1.0-1.2
×108Individual bacterium cell.
2. by claim 1 microorganism phosphate fertilizer, it is characterised in that: the suction of various bacterial strains on described carrier
Attached amount is identical;Root nodule bacteria are Semen sojae atricolor or peanut rhizobium.
3. by claim 1 microorganism phosphate fertilizer, it is characterised in that: described carrier is straw, humic acids
Or farm manure.
4. by claim 3 microorganism phosphate fertilizer, it is characterised in that: straw is to ferment through saccharomyces cerevisiae
After, stand-by after pulverizing and sieving;Humic acids was for pulverizing 80 mesh sieves;Farm manure is to send out through saccharomyces cerevisiae
Ferment, pulverizes and sieves after fermentation, then decomposes through Rhodopseudomonas spheroides, stand-by.
5. by claim 3 microorganism phosphate fertilizer, it is characterised in that be inoculated in the wine brewing on straw described in:
Yeast inoculum concentration is 0.5%;The described saccharomyces cerevisiae inoculum concentration being inoculated in farm manure is 0.5%, sends out
The inoculum concentration inoculation Rhodopseudomonas spheroides of 5% is pressed again after ferment.
6. the application of the microorganism phosphate fertilizer described in a claim 1, it is characterised in that: by described micro-
Biologic phosphorus fertilizer is manured into soil substrate, then long-term cropping;Wherein, every mu of soil matrix applies 5-10kg
Microorganism phosphate fertilizer.
7. the application of the microorganism phosphate fertilizer as described in claim 1 is characterized in that: by described microbe-P
Fertile and various crop seeds mix, and are then manured into soil in substrate, apply 5-10kg in every mu of soil matrix
Microorganism phosphate fertilizer.
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CN201510126501.9A CN106146111A (en) | 2015-03-23 | 2015-03-23 | A kind of microorganism phosphate fertilizer and application thereof |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1618772A (en) * | 2003-11-20 | 2005-05-25 | 郭伟光 | High efficiency biological organic fertilizer and its production technology |
CN101525254A (en) * | 2009-04-07 | 2009-09-09 | 华南农业大学 | Multifunctional biological compound fertilizer as well as preparation method and application thereof |
CN102531776A (en) * | 2011-12-23 | 2012-07-04 | 于永波 | Compound microbial fertilizer and method for producing same |
-
2015
- 2015-03-23 CN CN201510126501.9A patent/CN106146111A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1618772A (en) * | 2003-11-20 | 2005-05-25 | 郭伟光 | High efficiency biological organic fertilizer and its production technology |
CN101525254A (en) * | 2009-04-07 | 2009-09-09 | 华南农业大学 | Multifunctional biological compound fertilizer as well as preparation method and application thereof |
CN102531776A (en) * | 2011-12-23 | 2012-07-04 | 于永波 | Compound microbial fertilizer and method for producing same |
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