CN106069929B - Mystus fish interspecific hybridization method - Google Patents
Mystus fish interspecific hybridization method Download PDFInfo
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- CN106069929B CN106069929B CN201610468713.XA CN201610468713A CN106069929B CN 106069929 B CN106069929 B CN 106069929B CN 201610468713 A CN201610468713 A CN 201610468713A CN 106069929 B CN106069929 B CN 106069929B
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- 241001200292 Mystus Species 0.000 title claims abstract description 69
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000009403 interspecific hybridization Methods 0.000 title claims abstract description 13
- 238000002347 injection Methods 0.000 claims abstract description 50
- 239000007924 injection Substances 0.000 claims abstract description 50
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 241000251468 Actinopterygii Species 0.000 claims abstract description 28
- 238000012258 culturing Methods 0.000 claims abstract description 22
- 241000024416 Hemibagrus nemurus Species 0.000 claims abstract description 17
- 230000002196 ecbolic effect Effects 0.000 claims abstract description 17
- 241000024414 Hemibagrus Species 0.000 claims abstract description 13
- 230000009027 insemination Effects 0.000 claims abstract description 10
- 238000011534 incubation Methods 0.000 claims abstract description 9
- 235000013601 eggs Nutrition 0.000 claims abstract description 7
- 210000000582 semen Anatomy 0.000 claims abstract description 6
- 230000012447 hatching Effects 0.000 claims description 2
- 230000000384 rearing effect Effects 0.000 claims description 2
- 238000007796 conventional method Methods 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 238000012214 genetic breeding Methods 0.000 abstract description 2
- 241000399016 Hemibagrus wyckioides Species 0.000 description 6
- 241000595940 Notostraca Species 0.000 description 6
- 241000894007 species Species 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 3
- 235000013332 fish product Nutrition 0.000 description 3
- 238000011031 large-scale manufacturing process Methods 0.000 description 3
- 210000004379 membrane Anatomy 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 241001282157 Polymixia lowei Species 0.000 description 1
- 241000252496 Siluriformes Species 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000013332 literature search Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000004303 peritoneum Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a method for interspecific hybridization of hemibagrus fish, belonging to the technical field of aquatic product genetic breeding. Taking the Mystus wyckioides as male parent and the Mystus wyckioides as female parent, carrying out secondary oxytocic injection on the female Mystus wyckioides, and carrying out primary LRH-A injection2Second injection of LRH-A2+ DOM; into the female fish of Mystus nemurusInjecting LRH-A into Mystus wyckioides while injecting oxytocic for the second time2+ DOM, respectively culturing in different spawning ponds, keeping the water temperature of the two ponds at 25-27 ℃, culturing for 10-11 hours, carrying out artificial egg squeezing on mature female mystus nemurus, dissecting the spermary of the male mystus, collecting semen, and carrying out dry artificial insemination; and (4) sending the fertilized eggs into a flowing water tank, and carrying out artificial incubation and fry cultivation in flowing water with the water temperature of 25-27 ℃ to obtain the hybridized mystus fry. The hybrid obtained by the invention has good body color, high growth speed and strong temperature adaptability, and is beneficial to culture.
Description
Technical Field
The invention relates to a method for interspecific hybridization of hemibagrus fish, belonging to the technical field of aquatic product genetic breeding.
Background
The more mystus has the learning name ofM.PluriradiatusThe local names are white beard male and bent silk. Classified as belonging to the genera Siluriformes, Pseudobageraceae, Pagrudae, MystusMystusAnd is distributed in the red river system.
Mystus nemurus, entitled "Mystus nemurusM.wyckioidesThe local name is long beard fish. Classification is also in the same genus as Yue Wu, and is named because the upper leaf of the caudad fin is elongated into filaments. The Chinese famous species in Yunnan province, the Chinese famous species are distributed in the tributary of the water system in the lancang river, the Chinese famous species in the dry stream in the lancang river are rare, and the Chinese famous species are distributed in the water system of the Mei public river abroad.
Mystus fish is tender and white in meat quality, delicious in taste, white in peritoneum and free of muscle stabs, and is a fish product with good taste.
The mystus nemurus is a large fish, the maximum individual can reach more than 10 kg, the large-scale production technology of the seedlings is mature, but the large-scale production technology has a long culture period and high requirement on water temperature due to large market specification. The mystus wyckioides is a medium-sized and small fish, the body color is yellow, the size of the fish on the market is small, the meat quality is better than that of the mystus wyckioides, but the large-scale production difficulty of the seedlings is high due to the small fish, and the growth is slow. Therefore, there is a need to develop scientific research and experiments on mystus wyckioides and mystus wyckioides to obtain a new variety of fish in mystus, and provide people with a rich fish product of mystus that integrates nutrition and taste. Through the literature search, the same public reports as the invention are not found.
Disclosure of Invention
The invention aims to provide a method for interspecific hybridization of hemibagrus fish, so that hemibagrus fish products which are good in body color, high in growth speed, strong in temperature adaptability and beneficial to culture are obtained by hybridization among species of the same genus, and effective ways and technical supports are provided for large-scale and industrial development of hemibagrus fish culture.
The invention is realized by the following technical scheme: a method for interspecific hybridization of fish of the genus Mystus, characterized in that Mystus wyckioides is used as a male parent and Mystus wyckioides is used as a female parent, and the method comprises the following steps:
A. the following oxytocic injections were performed on female mystus wyckioides: first injection of LRH-A2The injection amount is 3 mug/kg; second injection of LRH-A2+DOM，LRH-A2The injection amount of (2) is 7 mug/kg, and the injection amount of DOM is 5 mg/kg; and after the first injection, the second injection is carried out at an interval of 12 h;
B. injecting LRH-A into the Mystus wyckioides female fish in the step A while injecting the oxytocic for the second time into the Mystus wyckioides female fish in the step A2+DOM，LRH-A2The injection amount of (2) is 10 mu g/kg, and the injection amount of DOM is 5 mg/kg;
C. respectively culturing the female mystus nemurus and the male mystus wyckioides which are artificially injected with the oxytocic in the step A and the step B in different spawning ponds, keeping the water temperatures of the two ponds consistent, and culturing for 10-11 hours at the water temperature of 25-27 ℃ to ensure that the female mystus wyckioides and the male mystus wyckioides are mature;
D. carrying out artificial oozing on the mature female mystus wyckioides, dissecting the spermary of the mystus wyckioides, collecting semen, and carrying out dry artificial insemination;
E. and D, conveying the fertilized eggs in the step D into a flowing water tank, and carrying out artificial incubation and fry cultivation in flowing water with the water temperature of 25-27 ℃ until the hybridized mystus fry is obtained.
And C, culturing in the step C only by controlling the water temperature, and culturing the rest in a conventional manner.
The dry artificial insemination of the step D is conventional artificial insemination.
The launder of the step E is conventional equipment, and the hatching and the fry rearing are conventional technologies.
The female mystus wyckioides and the male mystus wyckioides are wild or cultured in a pond.
And E, culturing the fish fry of the hemibagrus genus obtained in the step E for 30 days in a conventional manner to obtain the fish fry of the hemibagrus genus with the weight of 0.5g, wherein the fish fry has good body color, strong temperature adaptability and high growth speed in a culturing process at a fry culturing stage.
The invention has the following advantages and effects: by adopting the technical scheme, a new variety of hemibagrus fish can be obtained by interspecific hybridization of hemibagrus, the hemibagrus fish with the weight of 0.5g can be obtained after 30 days of conventional culture, and the obtained hybrid offspring has the advantages of good body color, high growth speed and strong temperature adaptability, is very beneficial to culture, and provides an effective way and technical support for large-scale and industrialized development of hemibagrus fish culture.
Detailed Description
The invention is further illustrated by the following examples.
Example 1
A method for interspecific hybridization of Mystus fishes, which takes 200g of wild Mystus wyckioides as a male parent and 5+ Mystus wyckioides cultivated in a pond as a female parent, comprises the following steps:
A. the following oxytocic injections were performed on female mystus wyckioides: LRH-A2 was injected for the first time, at an injection rate of 3 μ g/kg; injecting LRH-A2+ DOM for the second time, wherein the injection amount of LRH-A2 is 7 mu g/kg, and the injection amount of DOM is 5 mg/kg; and after the first injection, the second injection is carried out at an interval of 12 h;
B. while injecting the second oxytocic into the female hemibagrus wyckioides in the step A, injecting LRH-A2+ DOM into the male hemibagrus wyckioides, wherein the injection amount of LRH-A2 is 10 mu g/kg, and the injection amount of DOM is 5 mg/kg;
C. c, culturing the female mystus nemurus and the male mystus wyckioides in different spawning ponds respectively after the artificial injection of the oxytocic is finished in the step A and the step B, keeping the water temperatures of the two ponds at 25 ℃, and culturing for 11 hours to ensure that the mystus nemurus female fish is mature;
D. carrying out conventional artificial oozing on the mature female mystus nemurus, dissecting the spermary of the male mystus nemurus, collecting semen, and carrying out dry artificial insemination;
E. d, feeding the fertilized eggs in the step D into a conventional flowing water tank, carrying out conventional artificial incubation in flowing water with the water temperature of 25 ℃, and finishing the artificial incubation after the membrane is taken out after 43 hours; feeding fairy shrimp nauplii after two days, continuously feeding the fairy shrimp nauplii, and culturing for 15 days to obtain 2.0cm of fish hybrid fry of the genus Mystus;
F. the fish fry is cultured regularly and cultured for 15 days to obtain the Mystus hybridus fry with weight of 0.5g and body length of 5cm, and the fish fry has good body color, strong temperature adaptability and fast growth speed in the culture process.
Example 2
A method for interspecific hybridization of Mystus fishes, which takes Mystus wyckioides cultured in a pond with the weight of 200g as a male parent and 5+ Mystus wyckioides cultured in a pond as a female parent, comprises the following steps:
A. the following oxytocic injections were performed on female mystus wyckioides: LRH-A2 was injected for the first time, at an injection rate of 3 μ g/kg; injecting LRH-A2+ DOM for the second time, wherein the injection amount of LRH-A2 is 7 mu g/kg, and the injection amount of DOM is 5 mg/kg; and after the first injection, the second injection is carried out at an interval of 12 h;
B. while injecting the second oxytocic into the female hemibagrus wyckioides in the step A, injecting LRH-A2+ DOM into the male hemibagrus wyckioides, wherein the injection amount of LRH-A2 is 10 mu g/kg, and the injection amount of DOM is 5 mg/kg;
C. c, culturing the female mystus nemurus and the male mystus wyckioides in different spawning ponds respectively after the artificial injection of the oxytocic is finished in the step A and the step B, keeping the water temperatures of the two ponds at 27 ℃, and culturing for 10 hours to ensure that the mystus nemurus female fish is mature;
D. carrying out conventional artificial oozing on the mature female mystus nemurus, dissecting the spermary of the male mystus nemurus, collecting semen, and carrying out dry artificial insemination;
E. d, feeding the fertilized eggs in the step D into a conventional flowing water tank, carrying out conventional artificial incubation in flowing water with the water temperature of 27 ℃, and starting membrane production after 43 hours to finish artificial incubation; feeding fairy shrimp nauplii after two days, continuously feeding the fairy shrimp nauplii, and culturing for 15 days to obtain 2.0cm of fish hybrid fry of the genus Mystus;
F. the fish fry is cultured regularly and cultured for 15 days to obtain the Mystus hybridus fry with weight of 0.5g and body length of 5cm, and the fish fry has good body color, strong temperature adaptability and fast growth speed in the culture process.
Example 3
A method for interspecific hybridization of Mystus fishes, which takes Mystus wyckioides cultured in a pond with the weight of 200g as a male parent and 5+ Mystus wyckioides cultured in a pond as a female parent, comprises the following steps:
A. the following oxytocic injections were performed on female mystus wyckioides: LRH-A2 was injected for the first time, at an injection rate of 3 μ g/kg; injecting LRH-A2+ DOM for the second time, wherein the injection amount of LRH-A2 is 7 mu g/kg, and the injection amount of DOM is 5 mg/kg; and after the first injection, the second injection is carried out at an interval of 12 h;
B. while injecting the second oxytocic into the female hemibagrus wyckioides in the step A, injecting LRH-A2+ DOM into the male hemibagrus wyckioides, wherein the injection amount of LRH-A2 is 10 mu g/kg, and the injection amount of DOM is 5 mg/kg;
C. c, culturing the female mystus nemurus and the male mystus wyckioides in different spawning ponds respectively after the artificial injection of the oxytocic is finished in the step A and the step B, keeping the water temperatures of the two ponds at 26 ℃, and culturing for 10 hours to ensure that the mystus nemurus female fish is mature;
D. carrying out conventional artificial oozing on the mature female mystus nemurus, dissecting the spermary of the male mystus nemurus, collecting semen, and carrying out dry artificial insemination;
E. d, feeding the fertilized eggs in the step D into a conventional flowing water tank, carrying out conventional artificial incubation in flowing water with the water temperature of 26 ℃, and starting membrane production after 43 hours to finish artificial incubation; feeding fairy shrimp nauplii after two days, continuously feeding the fairy shrimp nauplii, and culturing for 15 days to obtain 2.0cm of fish hybrid fry of the genus Mystus;
F. the fish fry is cultured regularly and cultured for 15 days to obtain the Mystus hybridus fry with weight of 0.5g and body length of 5cm, and the fish fry has good body color, strong temperature adaptability and fast growth speed in the culture process.
Claims (5)
1. A method for interspecific hybridization of fish of the genus Mystus, characterized in that Mystus wyckioides is used as a male parent and Mystus wyckioides is used as a female parent, and the method comprises the following steps:
A. the following oxytocic injections were performed on female mystus wyckioides: first injection of LRH-A2The injection amount is 3 mug/kg; second injection of LRH-A2+DOM，LRH-A2The injection amount of (2) is 7 mug/kg, and the injection amount of DOM is 5 mg/kg; and after the first injection, the second injection is carried out at an interval of 12 h;
B. injecting LRH-A into the Mystus wyckioides female fish in the step A while injecting the oxytocic for the second time into the Mystus wyckioides female fish in the step A2+DOM，LRH-A2The injection amount of (2) is 10 mu g/kg, and the injection amount of DOM is 5 mg/kg;
C. respectively culturing the female mystus nemurus and the male mystus wyckioides which are artificially injected with the oxytocic in the step A and the step B in different spawning ponds, keeping the water temperatures of the two ponds consistent, and culturing for 10-11 hours at the water temperature of 25-27 ℃ to ensure that the female mystus wyckioides and the male mystus wyckioides are mature;
D. carrying out artificial oozing on the mature female mystus wyckioides, dissecting the spermary of the mystus wyckioides, collecting semen, and carrying out dry artificial insemination;
E. and D, conveying the fertilized eggs in the step D into a flowing water tank, and carrying out artificial incubation and fry cultivation in flowing water with the water temperature of 25-27 ℃ until the hybridized mystus fry is obtained.
2. The method of interspecies cross of fish of the genus mystus according to claim 1, wherein the culturing in step C requires only water temperature control, and the rest is conventional culturing.
3. The method for interspecific hybridization of hemibagrus fish according to claim 1, wherein said dry artificial insemination in step D is conventional artificial insemination.
4. The method of interspecific hybridization of hemibagrus fish according to claim 1, wherein said flow tank of step E is a conventional facility, and hatching and fry rearing are conventional techniques.
5. The method of interspecific crossing of Mystus fish of claim 1, wherein the female Mystus wyckioides and the male Mystus wyckioides are wild or are pond-cultured.
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| CN101574068A (en) * | 2009-06-12 | 2009-11-11 | 云南省渔业科学研究院 | Artificial taming method of pond with wild bagarius yarrelli |
| CN101695288A (en) * | 2009-10-22 | 2010-04-21 | 云南省渔业科学研究院 | Artificial propagation method of Mystus wyckioides |
| CN103404469B (en) * | 2013-08-26 | 2015-08-05 | 华能澜沧江水电有限公司 | The artificial fecundation method of a kind of Ju Po |
| CN105104263B (en) * | 2015-08-25 | 2019-01-11 | 华能澜沧江水电股份有限公司 | A kind of ash schizothoracin artificial fecundation method |
| CN105028284B (en) * | 2015-09-08 | 2019-10-01 | 云南省渔业科学研究院 | A kind of whole artificial propagation method of mystus nemurus |
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