CN105028284B - A kind of whole artificial propagation method of mystus nemurus - Google Patents
A kind of whole artificial propagation method of mystus nemurus Download PDFInfo
- Publication number
- CN105028284B CN105028284B CN201510565417.7A CN201510565417A CN105028284B CN 105028284 B CN105028284 B CN 105028284B CN 201510565417 A CN201510565417 A CN 201510565417A CN 105028284 B CN105028284 B CN 105028284B
- Authority
- CN
- China
- Prior art keywords
- parent population
- artificial
- individual
- fish
- male
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 26
- 241000024416 Hemibagrus nemurus Species 0.000 title claims abstract description 23
- 241000251468 Actinopterygii Species 0.000 claims abstract description 21
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 11
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 11
- 230000004720 fertilization Effects 0.000 claims abstract description 11
- 210000004681 ovum Anatomy 0.000 claims abstract description 11
- 238000009395 breeding Methods 0.000 claims abstract description 10
- 230000001488 breeding effect Effects 0.000 claims abstract description 10
- 210000000582 semen Anatomy 0.000 claims abstract description 8
- 230000012447 hatching Effects 0.000 claims abstract description 7
- 230000000384 rearing effect Effects 0.000 claims abstract description 7
- 238000002347 injection Methods 0.000 claims description 12
- 239000007924 injection Substances 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 241000208125 Nicotiana Species 0.000 claims description 4
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 4
- 238000009399 inbreeding Methods 0.000 abstract description 4
- 230000002068 genetic effect Effects 0.000 abstract description 2
- 230000001850 reproductive effect Effects 0.000 abstract description 2
- 230000027272 reproductive process Effects 0.000 abstract description 2
- 230000009027 insemination Effects 0.000 description 5
- 235000013601 eggs Nutrition 0.000 description 4
- 241000252233 Cyprinus carpio Species 0.000 description 2
- 241000276701 Oreochromis mossambicus Species 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 230000002051 biphasic effect Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000032696 parturition Effects 0.000 description 2
- 241001441703 Bagridae Species 0.000 description 1
- 241001200292 Mystus Species 0.000 description 1
- 241000252496 Siluriformes Species 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002196 ecbolic effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to a kind of whole artificial propagation methods of mystus nemurus, speed of growth individual with the obvious advantage is selected as reserve parent fish from the F1 generation group that wild type breeding obtains, gender is distinguished after reserve parent fish 2+ age, then the advantage individual of selection is cultivated, until being used as artificial parent population after 3+ age；Male and female parent population of the individual combined from different wild types as whole artificial propagation is selected in artificial parent population；Ocyodinic is injected to female parent population, male parent population injects while injecting at second of female parent population.Dry method of artificial fertilization is carried out by the way of cuing open milter and spermary collecting semen, raun is taken manually to squeeze ovum, and routinely carries out hatching and seed rearing.The present invention can not only protect wild type; and the cost for obtaining wild parent population can be saved; significantly reduce manual labor; improve reproductive efficiency; the excellent individual of energy M8003 line growth traits is bred; genetic diversity is kept, avoids the problem that inbreeding in conventional reproductive process, germ plasm resource are degenerated.
Description
Technical field
The present invention relates to a kind of whole artificial propagation methods of mystus nemurus, belong to technical field of aquaculture.
Background technique
Mystus nemurus, scientific name areMystus wyckioides Chaux etFang, the entitled fish that grows a beard in place.In classification
Belong to Silurformes Siluriformes, Chang section Bagridae, Of-digestive-tract categoryMystus, gain the name due to leaf extends into silk on its tail fin.?
The country is the indigenous species in Yunnan, is distributed in the water-based tributary in the Lancang River, rare in the mainstream of the Lancang River；Foreign countries are distributed in Mekong
Water-based downstream.Mystus nemurus body colour is beautiful, and back is cyan, and side is general light blue, gradually turns shallow, until abdomen is white.Dorsal fin,
Abdomeinal fin, anal fin outer rim are slightly red, and tail fin is red.Its fine and tender taste is snow-white, delicious flavour, and thorn, peritonaeum are white between no flesh, and maximum
Body is up to 10 kilograms or more.
Currently, the artificial fecundation method of mystus nemurus is both needed to the spermary for directly taking wild milter as ovum insemination, Er Qieye
Raw milter obtain when be difficult to determine milter age and growing state, can not directive breeding excellent variety, to protect and developing silk
This Wild fish resources of tail Of-digestive-tract, breeding mystus nemurus breed variety, it is necessary to develop a kind of whole artificial propagation method.
Summary of the invention
Need directly to cut open the problems such as at high cost, manual labor is big caused by killing wild parent population in the prior art to overcome, this
Invention provides a kind of method by whole artificial propagation acquisition mystus nemurus F2 for fry, and this method has protection wild type, can
The advantages of being bred with the individual that M8003 line growth traits is excellent, keeping genetic diversity, avoid inbreeding is fitted simultaneously
In producing seed in enormous quantities for cultivating, good basis has been established for mystus nemurus breed breeding.
The present invention is realized by following technical proposal: a kind of whole artificial propagation method of mystus nemurus, by following each step:
A, speed of growth individual with the obvious advantage is selected from the F1 generation group that wild type breeding obtains as standby parent
Fish numbers reserve parent fish and records, distinguishes gender after reserve parent fish 2+ age, then chooses that the speed of growth is fast, cut tobacco is bright on tail portion
Aobvious individual feeds fish block to the individual of selection and cultivates, until being used as artificial parent population after 3+ age；
B, select the individual combined from different wild types as whole artificial propagation in the artificial parent population of step A
Male and female parent population；
C, water temperature stability injects ocyodinic to the female parent population of step B: injection 3 μ of LRH-A2 for the first time at 25 DEG C or more
After g/kg, interval time 12h, second of injection 7 μ g+DOM 5mg/kg of LRH-A2；To the male parent population of step B in female parent population
Biphasic injection injects 10 μ g+DOM 5mg/kg of LRH-A2 simultaneously；Effect time is 10~11h；
D, to the male parent population of step C and female parent population by the way of cuing open milter and spermary collecting semen, raun is taken manually to squeeze ovum
Dry method of artificial fertilization is carried out, and hatching and seed rearing are routinely carried out using gutter channel.
The fish block that feeds is to feed carp, Tilapia mossambica fish block.
The advantages of present invention has and effect: the present invention is directed to carry out the whole artificial propagation of mystus nemurus, not only wild parent population
Usage quantity it is few, and can in high volume obtain mystus nemurus F2 for seed.Wild parent population is killed without cuing open, wild parent can not only be protected
This, and the cost for obtaining wild parent population can be saved.The fry of identical quantity is bred, required wild parent population quantity is few, cuts open murder
Work parent population quantity is also reduced, hence it is evident that is reduced manual labor, is improved reproductive efficiency.And artificial propagation obtains F1 generation, it can
Age and the growing state for determining F1 generation parent population, thus, it is possible to the excellent individuals of M8003 line growth traits to be bred, and keep losing
Diversity is passed, avoids the problem that inbreeding in conventional reproductive process, germ plasm resource are degenerated, while being suitable for producing seed in enormous quantities
Good basis has been established for cultivating, and for mystus nemurus breed breeding.
Specific embodiment
Below by embodiment, the present invention will be further described.
Embodiment 1
A, the F1 generation group that wild type breeding obtains therefrom selects the speed of growth by seed rearing to 15~20cm
Individual with the obvious advantage is numbered reserve parent fish and is recorded, distinguish gender after reserve parent fish 2+ age, then choose as reserve parent fish
Cut tobacco is significantly individual fastly, on tail portion for the speed of growth, and it is 1:2(due to that need to take spermary to milter that male and female, which choose quantitative proportion,
Milter is caused to lose, therefore more options raun), fish block (carp, Tilapia mossambica fish block) is fed to the individual of selection and is cultivated, until 3+
Artificial parent population is used as after age；
B, select the individual combined from different wild types as whole artificial propagation in the artificial parent population of step A
Male and female parent population；
C, 25 DEG C of water temperature when, ocyodinic is injected to the female parent population of step B: injection 3 μ g/kg of LRH-A2 for the first time, when interval
Between after 12h, inject 7 μ g+DOM 5mg/kg of LRH-A2 second；The male parent population of step B is injected simultaneously at second of female parent population
Inject 10 μ g+DOM 5mg/kg of LRH-A2；Effect time is 11h；
D, until when Effect time, male parent population and female parent population to step C take spermary collecting semen, raun using cuing open milter
The mode of artificial crowded ovum carries out dry method of artificial fertilization (sperm of 1 tail milter is the insemination of 4 tail rauns), and is incubated using gutter channel
Change and seed rearing.
Embodiment 2
A, the F1 generation group that wild type breeding obtains therefrom selects the speed of growth by seed rearing to 15~20cm
Individual with the obvious advantage is numbered reserve parent fish and is recorded, distinguish gender after reserve parent fish 2+ age, then choose as reserve parent fish
Cut tobacco is significantly individual fastly, on tail portion for the speed of growth, and it is 1:2(due to that need to take spermary to milter that male and female, which choose quantitative proportion,
Milter is caused to lose, therefore more options raun), fish block is fed to the individual of selection and is cultivated, until being used as artificial parent population after 3+ age；
B, select the individual combined from different wild types as whole artificial propagation in the artificial parent population of step A
Male and female parent population；
C, 26 DEG C of water temperature when, ocyodinic is injected to the female parent population of step B: injection 3 μ g/kg of LRH-A2 for the first time, when interval
Between after 12h, inject 7 μ g+DOM 5mg/kg of LRH-A2 second；The male parent population of step B is injected simultaneously at second of female parent population
Inject 10 μ g+DOM 5mg/kg of LRH-A2；Effect time is 10h；
D, until when Effect time, male parent population and female parent population to step C take spermary collecting semen, raun using cuing open milter
The mode of artificial crowded ovum carries out dry method of artificial fertilization (sperm of 1 tail milter is the insemination of 6 tail rauns), and is incubated using gutter channel
Change and seed rearing.
Result of implementation shows that this method can obtain mystus nemurus F2 for seed by complete artificial method high-volume.To show
Effect of the invention, following to be verified by comparative test:
One, subjects
1, test group: the whole artificial propagation of mystus nemurus is carried out as described in Example 2；
2, control group: referring to a kind of published patent application " artificial propagation side of mystus nemurus Mystus wyckioides
Method (200910095074.7) ".Ocyodinic, at 26 DEG C of water temperature, double injection are injected using biphasic injection to wild mystus nemurus
Interval time be 12h, Effect time be 10~11h；Dosage of hastening parturition is raun injection 3 μ g+DOM 2mg/ of LRH-A2 for the first time
kg；Second of injection 10 μ g+DOM 4mg+HCG 1000IU/kg of LRH-A2, milter dosage halve；Artificial insemination uses and cuts open hero
The mode that fish takes spermary collecting semen, raun manually to squeeze ovum carries out dry method of artificial fertilization；Artificial incubation is incubated using incubation tank flowing water
Change, the fertilized eggs cloth after insemination carries out shading hatching in brown piece.
Two, test result
1, test group: wild mystus nemurus totally 36 tails (female 18 tail, male 18 tails) are taken, in the F1 generation group of acquisition, to 18
Tail F1 generation raun injects ocyodinic, and spawning rate obtains 1,050,000, ovum up to 100% altogether；Ocyodinic is injected to 18 tail F1 generation milters；It takes
Cuing open 3 tail of F1 generation milter takes spermary collecting semen, raun manually to squeeze ovum dry method of artificial fertilization, and fertilization rate reached 91.2% obtains fertilized eggs
94.5 ten thousand.Statistics obtains 81.5 ten thousand tail of fry, hatching rate 86.2%.
2, control group: wild mystus nemurus totally 56 tails (female 28 tail, male 28 tails) are taken, are hastened parturition to the injection of 28 tails wild raun
Agent, spawning rate obtain 1,100,000, ovum up to 100% altogether；Ocyodinic is injected to the wild milter of 28 tails；Essence is taken using wild 12 tail of milter is cutd open
Nest collecting semen, raun manually squeeze ovum dry method of artificial fertilization, and fertilization rate reached 70.9% obtains fertilized eggs 780,000.Statistics obtains fry
68.8 ten thousand tails, hatching rate 88.2%.
Three, conclusion
Test group obtains fertilized eggs and is more than control group, and it is more to obtain fry.The method that control group uses, because needs are adopted
The method cut open the belly and take spermary is taken, a plurality of male mystus nemurus wild type is lost.Since mystus nemurus wild type acquisition difficulty is big, at
This is high, and seed is caused to hold at high price, and is unfavorable for promoting cultivation, and cuts open to kill wild parent population quantity and cut open greater than test group and kill F1
For the quantity of milter.Test group uses method provided by the invention, the results showed that and have and wild type is avoided to lose, directive breeding
Growth vigor is obvious, avoids the advantages of inbreeding；And cut open kill F1 generation milter quantity it is few, reduce manual labor；Test
Group improves the type and dosage of oxytocic drug, has the characteristics that rate of fertilization is high, hatching rate is high, low in cost, emergence is more.The present invention
The method of offer has substantive distinguishing features outstanding and significant progress.
Claims (1)
1. a kind of whole artificial propagation method of mystus nemurus, it is characterised in that pass through following each step:
A, speed of growth individual with the obvious advantage is selected from the F1 generation group that obtains of wild type breeding as reserve parent fish, it is right
Reserve parent fish number record, distinguishes gender after reserve parent fish 2+ age, then chooses that the speed of growth is fast, cut tobacco is significantly a on tail portion
Body feeds fish block to the individual of selection and cultivates, until being used as artificial parent population after 3+ age；
B, male and female of the individual combined from different wild types as whole artificial propagation are selected in the artificial parent population of step A
Parent population；
C, water temperature stability injects ocyodinic to the female parent population of step B at 25 DEG C or more: injection 3 μ g/kg of LRH-A2 for the first time,
After interval time 12h, second of injection 7 μ g+DOM 5mg/kg of LRH-A2；The male parent population of step B is infused at second of female parent population
It penetrates while injecting 10 μ g+DOM 5mg/kg of LRH-A2；Effect time is 10~11h；
D, the male parent population of step C and female parent population are carried out by the way of cuing open milter and spermary collecting semen, raun is taken manually to squeeze ovum
Dry method of artificial fertilization, and routinely carry out hatching and seed rearing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510565417.7A CN105028284B (en) | 2015-09-08 | 2015-09-08 | A kind of whole artificial propagation method of mystus nemurus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510565417.7A CN105028284B (en) | 2015-09-08 | 2015-09-08 | A kind of whole artificial propagation method of mystus nemurus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105028284A CN105028284A (en) | 2015-11-11 |
| CN105028284B true CN105028284B (en) | 2019-10-01 |
Family
ID=54435774
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510565417.7A Active CN105028284B (en) | 2015-09-08 | 2015-09-08 | A kind of whole artificial propagation method of mystus nemurus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105028284B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069928A (en) * | 2016-06-25 | 2016-11-09 | 华能澜沧江水电股份有限公司 | A kind of artificial fecundation method of fork-tail catfish |
| CN106069929B (en) * | 2016-06-25 | 2020-02-14 | 云南省渔业科学研究院 | Mystus fish interspecific hybridization method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695288A (en) * | 2009-10-22 | 2010-04-21 | 云南省渔业科学研究院 | Artificial propagation method of Mystus wyckioides |
| CN102613125A (en) * | 2012-04-26 | 2012-08-01 | 云南省渔业科学研究院 | Artificial insemination method of mystus nemurus |
| CN104782548A (en) * | 2015-04-24 | 2015-07-22 | 西双版纳飞龙渔业有限责任公司 | Hemibagrus nemurus pond high-yield breeding method |
-
2015
- 2015-09-08 CN CN201510565417.7A patent/CN105028284B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695288A (en) * | 2009-10-22 | 2010-04-21 | 云南省渔业科学研究院 | Artificial propagation method of Mystus wyckioides |
| CN102613125A (en) * | 2012-04-26 | 2012-08-01 | 云南省渔业科学研究院 | Artificial insemination method of mystus nemurus |
| CN104782548A (en) * | 2015-04-24 | 2015-07-22 | 西双版纳飞龙渔业有限责任公司 | Hemibagrus nemurus pond high-yield breeding method |
Non-Patent Citations (2)
| Title |
|---|
| 丝尾鳠人工繁殖与苗种培育试验;王丹 等;《水产科学》;20050831;第24卷(第8期);第35-36页 * |
| 丝尾鳠的驯养及人工繁殖初报;薛晨江 等;《水生态学杂志》;20100731;第3卷(第4期);第142-144页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105028284A (en) | 2015-11-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100464633C (en) | Erythtoculer ilishaefornis and megajobrama amblycephala crossbreeding method | |
| CN103109769A (en) | Loach fish fry artificial propagation method | |
| KR20130131776A (en) | Woorimatdag no. 2 and no. 3 produced by using korean native pure line chicken | |
| Brämick et al. | Testing of triploid tilapia (Oreochromis niloticus) under tropical pond conditions | |
| CN105684962B (en) | One kind sticks up mouth Culter and triangular bream hybrid preparation method | |
| CN101647417B (en) | High-intensity selecting and remaining method of good carp parents | |
| CN101703016A (en) | Technology for culturing unisexual hybrid scallop by utilizing purple scallop and bay scallop | |
| CN108377936B (en) | Large-scale production method of all-female mandarin fish | |
| CN107950438A (en) | A kind of quick feed acclimation method of Micropterus salmonoides | |
| CN104719199A (en) | Cross breeding method for pelteobagrus fulvidraco and pseudobagrus vachelli | |
| CN103329833A (en) | Cross breeding method for Japanese crucian carp and cyprinus carpio red | |
| CN104161000A (en) | Hybrid carp and pengze crucian carp hybrid breeding method | |
| CN103478023A (en) | Artificial propagation technology for Rhodeus sinensis | |
| CN105360031A (en) | Method for quickly establishing cross-bred XY holandric pelteobagrus fulvidraco | |
| CN110521637A (en) | A kind of egg-shaped pompano family full-sibs construction method | |
| CN106922610B (en) | Ternary hybridization optimization combination breeding method for local black pigs | |
| CN105028284B (en) | A kind of whole artificial propagation method of mystus nemurus | |
| Yeh et al. | Induced sex change, spawning and larviculture of potato grouper, Epinephelus tukula | |
| CN103875567A (en) | Artificial spawning method for osteoglossum bicirrhosum | |
| KR101169013B1 (en) | Method for mass producing artificial seed of Misgurnus sp. | |
| CN101584305A (en) | Method for breeding and culturing hybridized snakehead fish | |
| CN108925472A (en) | A kind of Bambusa and megalobrama amblycephala cross breeding method | |
| KR20140040336A (en) | Aquaculture method for a cynoglossus semilaevis | |
| CN101695288A (en) | Artificial propagation method of Mystus wyckioides | |
| KR101885049B1 (en) | Hybrid production mathod of fishing carp and goldfish |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |