CN105028284A - Completely-artificial hemibagrus wyckioides reproduction method - Google Patents
Completely-artificial hemibagrus wyckioides reproduction method Download PDFInfo
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- CN105028284A CN105028284A CN201510565417.7A CN201510565417A CN105028284A CN 105028284 A CN105028284 A CN 105028284A CN 201510565417 A CN201510565417 A CN 201510565417A CN 105028284 A CN105028284 A CN 105028284A
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- parent fish
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 241000399016 Hemibagrus wyckioides Species 0.000 title abstract 2
- 241000251468 Actinopterygii Species 0.000 claims abstract description 26
- 210000000582 semen Anatomy 0.000 claims abstract description 10
- 238000002347 injection Methods 0.000 claims description 25
- 239000007924 injection Substances 0.000 claims description 25
- 241000551708 Lamprolia victoriae Species 0.000 claims description 14
- 102000002322 Egg Proteins Human genes 0.000 claims description 10
- 108010000912 Egg Proteins Proteins 0.000 claims description 10
- 230000004720 fertilization Effects 0.000 claims description 10
- 210000004681 ovum Anatomy 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 230000012447 hatching Effects 0.000 claims description 6
- 230000000384 rearing effect Effects 0.000 claims description 6
- 241000208125 Nicotiana Species 0.000 claims description 4
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 4
- 238000011534 incubation Methods 0.000 abstract description 6
- 230000009027 insemination Effects 0.000 abstract description 6
- 230000002068 genetic effect Effects 0.000 abstract description 3
- 238000009399 inbreeding Methods 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 230000015556 catabolic process Effects 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 210000001550 testis Anatomy 0.000 abstract 1
- 238000009395 breeding Methods 0.000 description 7
- 230000001488 breeding effect Effects 0.000 description 7
- 241000252233 Cyprinus carpio Species 0.000 description 2
- 241000276701 Oreochromis mossambicus Species 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 241001441703 Bagridae Species 0.000 description 1
- 241001200292 Mystus Species 0.000 description 1
- 241000252496 Siluriformes Species 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000002051 biphasic effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002196 ecbolic effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a completely-artificial hemibagrus wyckioides reproduction method. According to the method, individuals with remarkable growth rate advantages are selected from F1 progenies obtained through wild parent reproduction to serve as reserved parent fish, gender distinguishing is conducted after the reserved parent fish is over two years old, and then superior individuals are selected for cultivation and taken as artificial parent fish after being bred to over three years old; individuals from different wild parent combinations are selected from the artificial parent fish to serve as male and female parent fish for completely-artificial reproduction; odinagogue is injected into the female parent fish, and odinagogue is also injected into the male parent fish when odinagogue is injected into the female parent fish for the second time; dry artificial insemination is conducted by dissecting the male fish to take out testes and acquire seminal fluid and conducting egg stripping on the female fish, and then incubation and fry cultivation are conducted by routine. By the adoption of the method, the wild parents can be protected, cost for obtaining the wild parent fish can be reduced, labor force is saved remarkably, reproduction efficiency is improved, individuals with good growth traits can be selected in a targeted mode for reproduction, genetic diversity is maintained, and the problems of inbreeding and germplasm resource degradation during conventional reproduction are solved.
Description
Technical field
The present invention relates to the whole artificial propagation method of a kind of tail Of-digestive-tract, belong to technical field of aquaculture.
Background technology
Silk tail Of-digestive-tract, formal name used at school is
mystuswyckioideschaux
etfang, the local fish that grows a beard by name.Classification belongs to Silurformes Siluriformes, Chang section Bagridae, Of-digestive-tract to belong to
mystus, gain the name because leaf on its tail fin extends into silk.Be the indigenous species in Yunnan at home, be distributed in the tributary of Lancang River water system, rare in master stream, the Lancang River; Be distributed in the downstream of Mekong water system abroad.Silk tail Of-digestive-tract body colour is attractive in appearance, and back is cyan, and side is general light blue, turns shallow gradually, is white to belly.Dorsal fin, abdomeinal fin, anal fin outer rim are slightly red, and tail fin is red.Its fine and tender taste is snow-white, delicious flavour, and be white without thorn, peritonaeum between flesh, maximum individuality can reach more than 10 kilograms.
At present; the artificial fecundation method of silk tail Of-digestive-tract all needs the spermary directly taking wild milter to be ovum insemination; and be difficult to the Age and growth situation determining milter during wild milter acquisition; cannot directive breeding improved seeds; for protection and this Wild fish resources of exploitation silk tail Of-digestive-tract; seed selection silk tail Of-digestive-tract breed variety, is necessary to develop a kind of whole artificial propagation method.
Summary of the invention
Need directly to cut open for overcoming in prior art the problems such as the cost that wild parent population causes is high, manual labor is large of killing; the invention provides a kind of by the method for whole artificial propagation acquisition silk tail Of-digestive-tract F2 for fry; the method has protection wild type; can breed by the excellent individuality of M8003 line growth traits; keep genetic diversity; avoid inbred advantage, be suitable for producing seed in enormous quantities for cultivation, for silk tail Of-digestive-tract breed breeding has established good basis simultaneously.
The present invention is realized by following technical proposal: the whole artificial propagation method of a kind of tail Of-digestive-tract, through following each step:
A, to breed the F1 generation colony obtained from wild type and select growth rate individuality with the obvious advantage parent population in support, to reserve parent fish number record, sex is distinguished after reserve parent fish 2+ age, choose cut tobacco on fast growth, afterbody more significantly individual, the individuality chosen fish block of throwing something and feeding is cultivated, as artificial parent population to 3+ age;
B, in the artificial parent population of steps A, select the individuality that combines from different wild type as the male and female parent population of whole artificial propagation;
When C, water temperature stability are more than 25 DEG C, the female parent population injection ocyodinic to step B: first time injection LRH-A23 μ g/kg, after interval time 12h, second time injection LRH-A27 μ g+DOM5mg/kg; In the injection of female parent population second time, LRH-A210 μ g+DOM5mg/kg is injected simultaneously to the male parent population of step B; Effect time is 10 ~ 11h;
D, the male parent population of step C and female parent population adopted and cuts open milter and get the mode that spermary collecting semen, raun manually squeeze ovum and carry out dry method of artificial fertilization, and adopt gutter channel to carry out hatching and seed rearing routinely.
Described fish block of throwing something and feeding is throw something and feed carp, Tilapia mossambica fish block.
The advantage that the present invention possesses and effect: the present invention is intended to carry out a whole artificial propagation of tail Of-digestive-tract, and not only the usage quantity of wild parent population is few, and a tail Of-digestive-tract F2 can be obtained in enormous quantities for seed.Killing wild parent population without the need to cuing open, can not only wild type be protected, and the cost obtaining wild parent population can be saved.The fry of breeding equal number, required wild parent population quantity is few, cuts open work parent population quantity of killing a person and also reduces, significantly reduce manual labor, improve reproductive efficiency.And artificial propagation obtains F1 generation, the Age and growth situation of F1 generation parent population can be determined, can breed by the excellent individuality of M8003 line growth traits thus, keep genetic diversity, avoid the problem of inbreeding in conventional reproductive process, germ plasm resource degeneration, be suitable for producing seed in enormous quantities for cultivation simultaneously, and established good basis for silk tail Of-digestive-tract breed breeding.
Embodiment
Below by embodiment, the present invention will be further described.
Embodiment 1
The F1 generation colony that the breeding of A, wild type obtains, by seed rearing to 15 ~ 20cm, therefrom select growth rate individuality with the obvious advantage parent population in support, to reserve parent fish number record, sex is distinguished after reserve parent fish 2+ age, choose cut tobacco on fast growth, afterbody more significantly individual, and male and female to choose quantitative proportion be that 1:2(causes milter to lose owing to need take spermary to milter, therefore more options raun), the individuality chosen fish block (carp, Tilapia mossambica fish block) of throwing something and feeding is cultivated, as artificial parent population to 3+ age;
B, in the artificial parent population of steps A, select the individuality that combines from different wild type as the male and female parent population of whole artificial propagation;
When C, water temperature 25 DEG C, the female parent population injection ocyodinic to step B: first time injection LRH-A23 μ g/kg, after interval time 12h, second time injection LRH-A27 μ g+DOM5mg/kg; In the injection of female parent population second time, LRH-A210 μ g+DOM5mg/kg is injected simultaneously to the male parent population of step B; Effect time is 11h;
D until Effect time time, the male parent population of step C and female parent population are adopted and cuts open milter and get the mode that spermary collecting semen, raun manually squeeze ovum and carry out dry method of artificial fertilization's (seminal fluid of 1 tail milter is 4 tail rauns inseminations), and adopt gutter channel to carry out hatching and seed rearing.
Embodiment 2
The F1 generation colony that the breeding of A, wild type obtains, by seed rearing to 15 ~ 20cm, therefrom select growth rate individuality with the obvious advantage parent population in support, to reserve parent fish number record, sex is distinguished after reserve parent fish 2+ age, choose cut tobacco on fast growth, afterbody more significantly individual, and male and female to choose quantitative proportion be that 1:2(causes milter to lose owing to need take spermary to milter, therefore more options raun), the individuality chosen fish block of throwing something and feeding is cultivated, as artificial parent population to 3+ age;
B, in the artificial parent population of steps A, select the individuality that combines from different wild type as the male and female parent population of whole artificial propagation;
When C, water temperature 26 DEG C, the female parent population injection ocyodinic to step B: first time injection LRH-A23 μ g/kg, after interval time 12h, second time injection LRH-A27 μ g+DOM5mg/kg; In the injection of female parent population second time, LRH-A210 μ g+DOM5mg/kg is injected simultaneously to the male parent population of step B; Effect time is 10h;
D until Effect time time, the male parent population of step C and female parent population are adopted and cuts open milter and get the mode that spermary collecting semen, raun manually squeeze ovum and carry out dry method of artificial fertilization's (seminal fluid of 1 tail milter is 6 tail rauns inseminations), and adopt gutter channel to carry out hatching and seed rearing.
Result of implementation shows, this method obtains a tail Of-digestive-tract F2 for seed in enormous quantities by the method for total man's work.For showing effect of the present invention, followingly to be verified by comparative trial:
One, subjects
1, experimental group: carry out a whole artificial propagation of tail Of-digestive-tract by the method for embodiment 2;
2, control group: see published patent application " artificial fecundation method (200910095074.7) of a kind of tail Of-digestive-tract Mystuswyckioides ".Adopt biphasic injection injection ocyodinic to wild silk tail Of-digestive-tract, during water temperature 26 DEG C, the interval time of double injection is 12h, and Effect time is 10 ~ 11h; Dosage of hastening parturition is raun first time injection LRH-A23 μ g+DOM2mg/kg; Second time injection LRH-A210 μ g+DOM4mg+HCG1000IU/kg, milter dosage reduces by half; Artificial insemination adopts to be cutd open milter and gets spermary collecting semen, raun and manually squeeze the mode of ovum and carry out dry method of artificial fertilization; Artificial incubation adopts incubation tank Lotic hatching, and the fertilized egg cloth after insemination carries out shading hatching on brown sheet.
Two, result of the test
1, experimental group: take wild silk tail Of-digestive-tract totally 36 tails (female 18 tails, male 18 tails), in the F1 generation colony obtained, to 18 tail F1 generation raun injection ocyodinics, spawning rate reaches 100%, obtains 1,050,000, ovum altogether; To 18 tail F1 generation milter injection ocyodinics; Take to cut open F1 generation milter 3 tail and get spermary collecting semen, raun manually crowded ovum dry method of artificial fertilization, fertilization rate reached 91.2%, obtains 94.5 ten thousand, fertilized egg.Statistics obtains fry 81.5 ten thousand tail, and incubation rate is 86.2%.
2, control group: take wild silk tail Of-digestive-tract totally 56 tails (female 28 tails, male 28 tails), to 28 tail wild raun injection ocyodinic, spawning rate reaches 100%, obtains 1,100,000, ovum altogether; To 28 tail wild milter injection ocyodinic; Employing is cutd open wild milter 12 tail and is got spermary collecting semen, raun manually crowded ovum dry method of artificial fertilization, and fertilization rate reached 70.9%, obtains 780,000, fertilized egg.Statistics obtains fry 68.8 ten thousand tail, and incubation rate is 88.2%.
Three, conclusion
Experimental group obtains fertilized egg more than control group, and it is more to obtain fry.The method that control group adopts, getting the method for spermary because needing to take to cut open the belly, losing many male silk tail Of-digestive-tract wild types.Due to silk tail Of-digestive-tract wild type, to gather difficulty large, with high costs, causes seed to hold at high price, be unfavorable for promoting cultivation, and cut open and kill wild parent population quantity and be greater than experimental group and cut open the quantity of killing F1 generation milter.Experimental group adopts method provided by the invention, and result shows to have avoids wild type to lose, directive breeding growth vigor obviously, avoid inbred advantage; And it is few to cut open the quantity of killing F1 generation milter, reduces manual labor; Experimental group improves the kind of oxytocic drug and dosage, has that fertilization rate is high, incubation rate is high, with low cost a, feature such as many of emerging.Method provided by the invention has outstanding substantive distinguishing features and significant progress.
Claims (1)
1. a whole artificial propagation method of silk tail Of-digestive-tract, is characterized in that through following each step:
A, to breed the F1 generation colony obtained from wild type and select growth rate individuality with the obvious advantage parent population in support, to reserve parent fish number record, sex is distinguished after reserve parent fish 2+ age, choose cut tobacco on fast growth, afterbody more significantly individual, the individuality chosen fish block of throwing something and feeding is cultivated, as artificial parent population to 3+ age;
B, in the artificial parent population of steps A, select the individuality that combines from different wild type as the male and female parent population of whole artificial propagation;
When C, water temperature stability are more than 25 DEG C, the female parent population injection ocyodinic to step B: first time injection LRH-A23 μ g/kg, after interval time 12h, second time injection LRH-A27 μ g+DOM5mg/kg; In the injection of female parent population second time, LRH-A210 μ g+DOM5mg/kg is injected simultaneously to the male parent population of step B; Effect time is 10 ~ 11h;
D, the male parent population of step C and female parent population adopted and cuts open milter and get the mode that spermary collecting semen, raun manually squeeze ovum and carry out dry method of artificial fertilization, and carry out routinely hatching and seed rearing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510565417.7A CN105028284B (en) | 2015-09-08 | 2015-09-08 | A kind of whole artificial propagation method of mystus nemurus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510565417.7A CN105028284B (en) | 2015-09-08 | 2015-09-08 | A kind of whole artificial propagation method of mystus nemurus |
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| CN105028284A true CN105028284A (en) | 2015-11-11 |
| CN105028284B CN105028284B (en) | 2019-10-01 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069928A (en) * | 2016-06-25 | 2016-11-09 | 华能澜沧江水电股份有限公司 | A kind of artificial fecundation method of fork-tail catfish |
| CN106069929A (en) * | 2016-06-25 | 2016-11-09 | 云南省渔业科学研究院 | The method of one kind Fish intervarietal hybridization |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695288A (en) * | 2009-10-22 | 2010-04-21 | 云南省渔业科学研究院 | Artificial propagation method of Mystus wyckioides |
| CN102613125A (en) * | 2012-04-26 | 2012-08-01 | 云南省渔业科学研究院 | Artificial insemination method of mystus nemurus |
| CN104782548A (en) * | 2015-04-24 | 2015-07-22 | 西双版纳飞龙渔业有限责任公司 | Hemibagrus nemurus pond high-yield breeding method |
-
2015
- 2015-09-08 CN CN201510565417.7A patent/CN105028284B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695288A (en) * | 2009-10-22 | 2010-04-21 | 云南省渔业科学研究院 | Artificial propagation method of Mystus wyckioides |
| CN102613125A (en) * | 2012-04-26 | 2012-08-01 | 云南省渔业科学研究院 | Artificial insemination method of mystus nemurus |
| CN104782548A (en) * | 2015-04-24 | 2015-07-22 | 西双版纳飞龙渔业有限责任公司 | Hemibagrus nemurus pond high-yield breeding method |
Non-Patent Citations (2)
| Title |
|---|
| 王丹 等: "丝尾鳠人工繁殖与苗种培育试验", 《水产科学》 * |
| 薛晨江 等: "丝尾鳠的驯养及人工繁殖初报", 《水生态学杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069928A (en) * | 2016-06-25 | 2016-11-09 | 华能澜沧江水电股份有限公司 | A kind of artificial fecundation method of fork-tail catfish |
| CN106069929A (en) * | 2016-06-25 | 2016-11-09 | 云南省渔业科学研究院 | The method of one kind Fish intervarietal hybridization |
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| CN105028284B (en) | 2019-10-01 |
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