CN106062179A - 无血清培养基 - Google Patents
无血清培养基 Download PDFInfo
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- CN106062179A CN106062179A CN201580008299.8A CN201580008299A CN106062179A CN 106062179 A CN106062179 A CN 106062179A CN 201580008299 A CN201580008299 A CN 201580008299A CN 106062179 A CN106062179 A CN 106062179A
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0031—Serum-free culture media
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/36—Lipids
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Rheumatology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14155263.8 | 2014-02-14 | ||
| EP14155263 | 2014-02-14 | ||
| PCT/EP2015/053223 WO2015121471A1 (en) | 2014-02-14 | 2015-02-16 | Serum-free medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106062179A true CN106062179A (zh) | 2016-10-26 |
Family
ID=50101793
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580008299.8A Pending CN106062179A (zh) | 2014-02-14 | 2015-02-16 | 无血清培养基 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US10472605B2 (https=) |
| EP (1) | EP3074505B1 (https=) |
| JP (1) | JP2017506511A (https=) |
| CN (1) | CN106062179A (https=) |
| WO (1) | WO2015121471A1 (https=) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112342181A (zh) * | 2020-03-26 | 2021-02-09 | 中国人民解放军总医院第四医学中心 | 基于数控激光辐射的非入侵式的细胞成图案方法及系统 |
| CN113621567A (zh) * | 2021-07-09 | 2021-11-09 | 生物岛实验室 | 组合物及其应用、干细胞培养基及干细胞的培养方法 |
| CN114286860A (zh) * | 2019-08-29 | 2022-04-05 | 味之素株式会社 | 由含有间充质干细胞的生物来源细胞试样制造间充质干细胞的方法 |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102676761B1 (ko) * | 2015-12-03 | 2024-06-18 | 다카라 바이오 가부시키가이샤 | 중간엽 줄기세포의 제조방법 |
| CN111334463B (zh) | 2016-05-18 | 2024-02-20 | 学校法人庆应义塾 | 类器官培养用细胞培养基、培养方法及类器官 |
| CN112322580B (zh) * | 2017-04-28 | 2022-03-22 | 北京赛斯达生物技术有限公司 | 一种间充质干细胞无血清培养基的应用 |
| KR102144593B1 (ko) * | 2017-06-07 | 2020-08-13 | 주식회사 엑소스템텍 | 인간줄기세포 유래 엑소좀을 포함하는 세포 배양용 무혈청 배지 조성물 |
| KR102185697B1 (ko) * | 2017-10-20 | 2020-12-03 | 본 테라퓨틱스 소시에테아노님 | 중간엽 줄기 세포의 분화 방법 |
| US20210123025A1 (en) * | 2018-07-03 | 2021-04-29 | Universidad Del País Vasco - Euskal Herriko Unibertsitatea | Cellular aggregates for use in vascularisation therapy |
| KR102337954B1 (ko) * | 2018-08-16 | 2021-12-10 | 차의과학대학교 산학협력단 | 뇌졸중 치료용 조성물 및 이를 스크리닝하는 방법 |
| AU2020310855A1 (en) * | 2019-07-08 | 2022-03-03 | Life Technologies Corporation | Compositions and methods for enhancing cell culture |
| JP2021040551A (ja) * | 2019-09-11 | 2021-03-18 | 富士フイルム株式会社 | 培地組成物、キット、間葉系幹細胞組成物の製造方法、間葉系幹細胞組成物、間葉系幹細胞、細胞培養上清および細胞外小胞 |
| JP7521738B2 (ja) * | 2020-05-18 | 2024-07-24 | キヤノン株式会社 | 目的細胞の生産方法、目的細胞による生産物の生産方法、および無血清培地 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5908782A (en) * | 1995-06-05 | 1999-06-01 | Osiris Therapeutics, Inc. | Chemically defined medium for human mesenchymal stem cells |
| US20100279412A1 (en) * | 2006-01-13 | 2010-11-04 | Japan Science And Technology Agency | Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof |
| WO2013054112A1 (en) * | 2011-10-10 | 2013-04-18 | Cell Guidance Systems Limited | Culture media for pluripotent stem cells |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61277623A (ja) * | 1985-05-10 | 1986-12-08 | マイルス・ラボラトリ−ス・インコ−ポレ−テツド | 哺乳動物の血漿または血清あるいはその分画からコレステロールに富んだ分画を単離しかつ精製する方法 |
| WO1998004681A2 (en) | 1996-07-25 | 1998-02-05 | Genzyme Corporation | Chondrocyte media formulations and culture procedures |
| TWI239352B (en) * | 1997-07-23 | 2005-09-11 | Takara Bio Inc | Gene transfer method with the use of serum-free medium |
| WO2001016294A2 (en) | 1999-08-27 | 2001-03-08 | Invitrogen Corporation | Metal binding compounds and their use in cell culture medium compositions |
| EP1458854B1 (en) | 2001-12-21 | 2010-04-14 | Mount Sinai Hospital | Cellular compositions and methods of making and using them |
| US20050265980A1 (en) | 2004-05-14 | 2005-12-01 | Becton, Dickinson And Company | Cell culture environments for the serum-free expansion of mesenchymal stem cells |
| CA2792802C (en) | 2010-03-10 | 2017-10-31 | Two Cells Co., Ltd. | Cell preparation containing mesenchymal stem cells, and method for producing same |
-
2015
- 2015-02-16 US US15/118,569 patent/US10472605B2/en active Active
- 2015-02-16 EP EP15707303.2A patent/EP3074505B1/en active Active
- 2015-02-16 JP JP2016551725A patent/JP2017506511A/ja not_active Ceased
- 2015-02-16 WO PCT/EP2015/053223 patent/WO2015121471A1/en not_active Ceased
- 2015-02-16 CN CN201580008299.8A patent/CN106062179A/zh active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5908782A (en) * | 1995-06-05 | 1999-06-01 | Osiris Therapeutics, Inc. | Chemically defined medium for human mesenchymal stem cells |
| US20100279412A1 (en) * | 2006-01-13 | 2010-11-04 | Japan Science And Technology Agency | Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof |
| WO2013054112A1 (en) * | 2011-10-10 | 2013-04-18 | Cell Guidance Systems Limited | Culture media for pluripotent stem cells |
Non-Patent Citations (2)
| Title |
|---|
| FENXI ZHANG等: "Lectin-like oxidized LDL receptor-1 expresses in mouse bone marrow-derived mesenchymal stem cells and stimulates their proliferation", 《EXPERIMENTAL CELL RESEARCH》 * |
| 郑铁生等: "《临床生物化学检验》", 31 January 2010, 中国医药科技出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114286860A (zh) * | 2019-08-29 | 2022-04-05 | 味之素株式会社 | 由含有间充质干细胞的生物来源细胞试样制造间充质干细胞的方法 |
| CN112342181A (zh) * | 2020-03-26 | 2021-02-09 | 中国人民解放军总医院第四医学中心 | 基于数控激光辐射的非入侵式的细胞成图案方法及系统 |
| CN112342181B (zh) * | 2020-03-26 | 2021-12-24 | 中国人民解放军总医院第四医学中心 | 基于数控激光辐射的非入侵式的细胞成图案方法及系统 |
| CN113621567A (zh) * | 2021-07-09 | 2021-11-09 | 生物岛实验室 | 组合物及其应用、干细胞培养基及干细胞的培养方法 |
| CN113621567B (zh) * | 2021-07-09 | 2024-01-30 | 生物岛实验室 | 组合物及其应用、干细胞培养基及干细胞的培养方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3074505B1 (en) | 2018-06-06 |
| US20170044489A1 (en) | 2017-02-16 |
| WO2015121471A1 (en) | 2015-08-20 |
| EP3074505A1 (en) | 2016-10-05 |
| JP2017506511A (ja) | 2017-03-09 |
| US10472605B2 (en) | 2019-11-12 |
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Application publication date: 20161026 |