CN106038802A - Preparation method of traditional Chinese medicinal composition for treating premature ovarian failure of women and traditional Chinese medicinal composition - Google Patents

Abstract

The invention discloses a preparation method of a traditional Chinese medicinal composition for treating premature ovarian failure of women. The preparation method comprises the following steps: in a step c, combining a component 1 with a component 2, and then putting a composition into a decompression concentration tank; recovering ethanol under reduced pressure, and concentrating until the concentration of liquid medicine is 0.6g of crude drug/ml; performing suction filtration until the relative density of filtrate is 1.06 at 20DEG C; adsorbing the filtrate by using a macroporous resin absorption column with the volume of 10L, and then using deionized water or distilled water which is 10 times of resin column volume for eluting; subsequently using 95 percent ethanol which is 5 times of resin column volume for eluting; collecting ethanol eluant, and removing a solvent to obtain medicinal powder, drying and charging into capsules. The traditional Chinese medicine for invigorating kidney for regulating menstruation has the functions of delaying hypogonadism and promoting recovery of ovarian functions; meanwhile, the traditional Chinese medicine for invigorating kidney has a certain adjusting effect for functions of female gonad axis.

Description

The Chinese medicine composition preparation method for the treatment of women's ovary senilism and combinations thereof thing

Technical field

The present invention relates to, containing the medicinal preparation deriving from plant composition, treat women's liver-fire band particularly to one Under Chinese medicine composition and preparation method thereof.

Background technology

Premature ovarian failure (POF) is the phenomenon of amenorrhea before referring to 40 years old that ovarian function failure is caused.Feature is primary Or secondary amenorrhea raises with blood Gonadotropin Level and estrogen level reduces, and a series of low female with in various degree Hormone symptom is such as: hectic fever hyperhidrosis, flush, hyposexuality etc..The average natural menopause age of women is 50～52 years old, menopause Age also exists the difference of race and Area distribution, but its absolute value is more or less the same.Coulam etc. sum up oneself of 1858 example women So amenorrhea situation, the POF incidence rate less than 40 years old is 1%, and the POF incidence rate less than 30 years old is 1 ‰.In primary amenorrhea, POF accounts for 10%～28%, in secondary amenorrhea, POF accounts for 4%～18%.Xu Ling etc. find that Beijing area women's POF incidence rate is 1.8%.By This is visible, and POF is the most much.

Premature ovarian failure (POF) is with change in menstrual cycle characteristics, estrogen deficiency, the rising of FSH level, decrease in estrogen for clinical special Point.Western medicine diagnose standard is to occur amenorrhea in more than at least 4 months before 40 years old, and has 2 times or above serum FSH ＞ 40U/L (2 Secondary inspection intervals more than 1 month), estradiol level ＜ 73.2pmol/L.Tcm diagnosis standard is cracked ends before 40 years old, and menstruation is dilute Few, gradually to amenorrhea or menolipsis suddenly, warm sweating, hectic fever is flushing, dysphoria with feverish sensation in the chest palms and soles, dizziness and tinnitus, soreness of the waist and knees or metapedes with pain, Urinate red dry stool, emaciated physique, vaginal dryness.B ultrasonic shows that uterus is less than normal or both sides ovary is less than normal, does not finds follicle or only monitors continuously Seeing the most developable little follicle of only a few, blood serum E2 low SI, FSH is significantly raised, red tongue or have crackle, tongue few, thready pulse Number or band string.

Premature ovarian failure accounts for the 1%～3.8% of women's total number of persons, is the commonly encountered diseases in gynecological endocrine field.Owing to this type of is suffered from Person shows as early sending out or primary amenorrhea, estrogen are lowly caused perimenopausal symptom and infertile, therefore belong to traditional Chinese medical science amenorrhea, early send out absolutely Warp, menopausal syndrome, the category such as infertile.Owing to suffering from a deficiency of the kidney, function senilism is appointed in punching, and the dyspoiesis of menses causes uterus vessels of the uterus (blood Sea) hollow, depletion of blood can descend and menopause praecox."Fu Qingzhu works on obstetric" cloud: " the most disconnected through water, it appears that kidney water declines and dries up ".Because women is with blood It is main, and " YIN often being in shortage, YANG often being in excess ", therefore treatment should focus on tonifying kidney and nourishing yin, promoting blood flow to regulate menstruation.In tonifying kidney and nourishing yin Fang Fang, Radix Rehmanniae Preparata is grown Kidney yin, benefit marrow is monarch drug.Premature ovarian failure has obvious heterogeneity to clinical manifestation from the cause of disease to the course of disease, carry out correct Etiological diagnosis, dialectical combines with differential diagnosis of diseases, takes corresponding treatment strategies, to improve healthy or to delay gonad old and feeble.

Modern study is thought, Bushen Tiaochong Chinese medicine has health invigorating, and regulation gonad axis and immunologic function, even delay gonad Old and feeble effect.Clinic shows, the clinical practice of tonifying kidney and nourishing yin side safely, effectively, treats premature ovarian failure curative effect certainly, untoward reaction Few, have certain effect in terms of protection, recovery ovarian function, its curative effect is equal to simple western medicine treatment, and toxic and side effects Less, low price.

The most best therapeutic modality is the method using traditional Chinese medical science conditioning and treating.Chinese traditional treatment can effectively solve disease Sick surface symptoms, can solve again the basic factor caused a disease thoroughly.Patient can also be the most suitable selection Physiotherapy.But, patient it is noted that must not at will medication, to carry out under the guidance of expert.For physiotherapy, also Limited, it is impossible to excessively, to be unfavorable for the state of an illness the most on the contrary, damage the health of patient.

Summary of the invention

The technical problem to be solved is, estrogen and progestogen replacement therapy is for young POF patient Very important, i.e. can alleviate low estrogen symptom and Urogenital atrophy (preparing for giving ovum embryo transfer), again may be used With prevention long term complication (osteoporosis, senile dementia etc.), the risk reduction 37% of colon cancer.But long-term HRT also has Certain risk, such as carcinoma of endometrium and the generation of breast carcinoma.The traditional Chinese medical science is particular about and is suited the remedy to the case, and individual variation is obvious, be expert Guidance under carry out.For physiotherapy, the most limited, it is impossible to excessively, to be unfavorable for the state of an illness the most on the contrary, damage the strong of patient Health.The present invention combines clinical experience for many years on the basis of traditional medicine, uses modern processing method meticulously to develop.This Bright each effective ingredient scientific compatibility to raw material medicine, focus of going directly, and instant effect, side effect be little, can efficient solution except patient Misery, reach the purpose of dispelling disease.

For solving above-mentioned technical barrier, the present invention provides a kind of Chinese medicine composition treating women's ovary senilism, by taking orally Medicine and external used medicine composition, described endo-medicine can include the crude drug of following weight portion: Radix Paeoniae Rubra 10～20 parts, Caulis Spatholobi 10～20 parts, Fructus Corni 10～20 parts, Herba Leonuri 10～20 parts, Radix Bupleuri 10～20 parts, Radix Arnebiae (Radix Lithospermi) 10～20 parts, Radix Rehmanniae Preparata 10～20 Part, Fructus Ligustri Lucidi 10～20 parts, Herba Ecliptae 10～20 parts, Fructus Schisandrae Chinensis 10～20 parts, Fructus Rubi 10～20 parts, Semen Cuscutae 10～20 Part, Herba Epimedii 10～20 parts, Cortex Moutan 10～20 parts, Radix Puerariae 10～20 parts；

Described external used medicine can include the crude drug of following parts by weight: Radix Arnebiae (Radix Lithospermi) 10～20 parts, Radix Sophorae Flavescentis 10～20 parts, Serpentis Machine tool 10～20 parts, Rhizoma Smilacis Glabrae 10～20 parts, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～20 parts, Folium Artemisiae Argyi 10～20 parts, Indigo Naturalis 10～20 parts, Spica Prunellae 10～ 20 parts, Borneolum Syntheticum 10～20 parts, Herba Euphrasiae Regelii 10～20 parts.

The Chinese medicine composition of described treatment women's ovary senilism, its described endo-medicine can also include following weight portion Crude drug: Radix Paeoniae Rubra 10～15 parts, Caulis Spatholobi 10～15 parts, Fructus Corni 10～15 parts, Herba Leonuri 10～15 parts, Radix Bupleuri 10～15 Part, Radix Arnebiae (Radix Lithospermi) 10～15 parts, Radix Rehmanniae Preparata 10～15 parts, Fructus Ligustri Lucidi 10～15 parts, Herba Ecliptae 10～15 parts, Fructus Schisandrae Chinensis 10～20 parts, cover Basin 10～20 parts, Semen Cuscutae 10～20 parts, Herba Epimedii 10～20 parts, Cortex Moutan 10～20 parts, Radix Puerariae 10～20 parts；

Described external used medicine can also include the crude drug of following parts by weight: Radix Arnebiae (Radix Lithospermi) 10～15 parts, Radix Sophorae Flavescentis 10～15 parts, Fructus Cnidii 10～15 parts, Rhizoma Smilacis Glabrae 10～15 parts, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～15 parts, Folium Artemisiae Argyi 10～20 parts, Indigo Naturalis 10～20 parts, Spica Prunellae 10 ～20 parts, Borneolum Syntheticum 10～20 parts, Herba Euphrasiae Regelii 10～20 parts.

The Chinese medicine composition of described treatment women's ovary senilism, its described endo-medicine can also include following weight portion Crude drug: Radix Paeoniae Rubra 10～20 parts, Caulis Spatholobi 10～20 parts, Fructus Corni 10～20 parts, Herba Leonuri 10～20 parts, Radix Bupleuri 10～20 Part, Radix Arnebiae (Radix Lithospermi) 10～20 parts, Radix Rehmanniae Preparata 10～20 parts, Fructus Ligustri Lucidi 10～20 parts, Herba Ecliptae 10～20 parts, Fructus Schisandrae Chinensis 10～15 parts, cover Basin 10～15 parts, Semen Cuscutae 10～15 parts, Herba Epimedii 10～15 parts, Cortex Moutan 10～15 parts, Radix Puerariae 10～15 parts；

Described external used medicine can also include the crude drug of following parts by weight: Radix Arnebiae (Radix Lithospermi) 10～20 parts, Radix Sophorae Flavescentis 10～20 parts, Fructus Cnidii 10～20 parts, Rhizoma Smilacis Glabrae 10～20 parts, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～20 parts, Folium Artemisiae Argyi 10～15 parts, Indigo Naturalis 10～15 parts, Spica Prunellae 10 ～15 parts, Borneolum Syntheticum 10～15 parts, Herba Euphrasiae Regelii 10～15 parts.

For solving above-mentioned technical problem, the present invention also provides for the preparation of a kind of Chinese medicine composition treating women's ovary senilism Method, its described endo-medicine is that the preparation process of capsule includes:

A, take crude drug put in ethanol soak, heat extract 2 times, for component 1；

B, medicinal residues extracting in water 2 times, thickening filtration, for component 2；

C, by above two component merge, concentrate, be dried to powder load capsule.

In described step a, can take in the ethanol of crude drug addition 5-10 times amount and soak 1-2 hour, heating extraction 2 times, 1-2 hour every time, united extraction liquid, remove supernatant after standing, then 100-120 mesh filters, then through the molecular weight that dams be 5000- The ultrafiltration post ultrafiltration of 10000, when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C, the extractum of 1.36, stand for standby use, become component 1。

In described step b, the medicinal residues that can take the crude drug that above-mentioned ethanol extraction is crossed add 10 times amount water, heating and refluxing extraction 2 times, each 1～2 hour, 2 extracting solution are merged and stands, become component 2.

In described step c, component 1 and the merging of component 2 can be then placed in concentrating under reduced pressure tank, recovered under reduced pressure second Alcohol, being concentrated into liquor strength is 0.6g crude drug/mL, when the relative density of sucking filtration to filtrate is 20 DEG C 1.06；Above-mentioned filtrate is through body Amass after the macroporous adsorptive resins for 10L adsorbs, with deionized water or the distilled water eluting of 10 times of resin column volumes, then with 5 times 95% ethanol elution of resin column volume, collects ethanol elution, removes solvent, obtains medicated powder and load capsule after drying.

For solving above-mentioned technical problem, the present invention also provides for the preparation of a kind of Chinese medicine composition treating women's ovary senilism Method, described external used medicine is that the preparation process of suppository includes:

A. take the Pseudobulbus Bletillae (Rhizoma Bletillae), Indigo Naturalis, the Borneolum Syntheticum way of distillation are vaporized into crystallization, pulverize dusting cover stand-by, as component 1；

B. remaining raw material ethanol is extracted, concentrate, as component 2；

C. adding substrate after component 1, component 2 being mixed to be in harmonious proportion, suppository, sterilization pack are made in sizing.

In described step a, crystal can be obtained, so first by the crude drug Pseudobulbus Bletillae (Rhizoma Bletillae), Indigo Naturalis, the distillation of Borneolum Syntheticum steam method Rear pulverizing, crosses 100-120 mesh sieve, standby as component 1；In described step b, again remaining crude drug can be shredded, be soaked Bubble has been placed in lid stainless steel cask, adds 70% ethanol, and quality is coarse grained 0.8-1 times, stirs, moistening airtight placement More than 1 hour, make fully to expand；Filter plate gauze bag bottom percolator is wrapped up after paving again by the medicine sample after moistening expansion Product are mixed pine and are done to dissipate, and then contain powder with rustless steel spoon, load percolator uniformly, fill 10-12 cm thick, even by T-bar pressure, then press Aforesaid operations, loading in layer, suitably pressurize, medicated powder loads 2/3 eminence that must not exceed percolator；Medicated powder face upper cover is not Rust steel orifice plate fastens, and opens the baiting valve below percolator, and puts a container, is then slowly added into 70% ethanol；To be discharged Air between medicated powder powder, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then Opening baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material outflow per minute 2～3ml, filtrate puts into liquid container In, and pour ethanol during emptying into liquid container；Percolate is merged and stands, as component 2；Component 1 component 2 mixes patent medicine Cream；Choose 50 DEG C～60 DEG C heating and melting of substrate, add ointment cooling and shaping obtained above and make class warhead shape suppository, ultraviolet Line is killed virus.

The preparation method of described treatment women's ovary senilism external suppository, its substrate used by described suppository can be selected from: One or more in semi-synthetic fatty acid glyceride, poloxamer, Polyethylene Glycol, lanoline；Described substrate is at temperature 50 C ～heating and melting under the conditions of 60 DEG C, adding the medicine mastic that described preparation method prepares, stirring in water bath is uniform, is mixed into thickness Liquid, is poured in plastic shape-forming pipe, inserts tampon, and cooling and shaping makes bullet-shaped suppository；Described tampon be afterbody with The tampon of cotton thread..

The present invention uses stype and tampon integrated technique, compensate for the deficiency of ordinary suppository, and ordinary suppository can not effectively send Reach the depths of vagina.Due to the self purification of vagina, in the presence of having foreign body, liquid secretion can be increased, at short notice will Medicine is got rid of external, had both reduced curative effect, and had extended again the course for the treatment of, and increase expense pollutes again underwear.And the present invention overcomes this Technical barrier, the suppository made can give full play to curative effect of medication, has safety good, the advantage that feasibility is good, focus of going directly, Reach the purpose that gets rid of illness.

Tonifying kidney and nourishing yin of the present invention, promoting blood flow to regulate menstruation.Radix Rehmanniae Preparata nourishing kidney-yin in tonifying kidney and nourishing yin side, benefit marrow is monarch drug.Fructus Corni, what The Radix Polygoni Multiflori, Semen Cuscutae liver and kidney tonifying, support essence and blood.Radix Arnebiae (Radix Lithospermi), Radix Paeoniae Rubra activating blood circulation to dissipate blood stasis, promoting the circulation of blood nourishing the liver blood, adjust punching to appoint.Herba Epimedii tonification liver Kidney.Fructus Ligustri Lucidi, Herba Ecliptae are ERZHI WAN, effect tonifying kidney and nourishing liver benefit essence.Radix Bupleuri nourishing YIN and clearing away heat pathogenic fire purging.Radix Puerariae clearing away heat and promoting production of body fluid, according to existing For pharmacological research Radix Puerariae, there is estrogen-like effects, for women's reproductive endocrine, there is regulation effect.All medicines share plays benefit altogether Kidney nourishing yin and activating blood, takes good care of punching and appoints effect.Invigorating kidney for regulating menstruation Chinese medicine has delay adenasthenia effect, has again promotion ovarian function Restitution, kidney-nourishing tcm drug has certain regulation effect to female gonad s function simultaneously, finds to mend by animal experiment study Renal drug has promotion ovary LH/HCG receptor to increase phenomenon.Chinese medicine there may also be promote fsh receptor produce effect, nourishing the liver and kidney, Benefit intensive culture blood Chinese medicine has the effect promoting follicular development.

Detailed description of the invention

Premature ovarian failure (POF) is with change in menstrual cycle characteristics, estrogen deficiency, the rising of FSH level, decrease in estrogen for clinical special Point.Western medicine diagnose standard is to occur amenorrhea in more than at least 4 months before 40 years old, and has 2 times or above serum FSH ＞ 40U/L (2 Secondary inspection intervals more than 1 month), estradiol level ＜ 73.2pmol/L.Tcm diagnosis standard is cracked ends before 40 years old, and menstruation is dilute Few, gradually to amenorrhea or menolipsis suddenly, warm sweating, hectic fever is flushing, dysphoria with feverish sensation in the chest palms and soles, dizziness and tinnitus, soreness of the waist and knees or metapedes with pain, Urinate red dry stool, emaciated physique, vaginal dryness.B ultrasonic shows that uterus is less than normal or both sides ovary is less than normal, does not finds follicle or only monitors continuously Seeing the most developable little follicle of only a few, blood serum E2 low SI, FSH is significantly raised, red tongue or have crackle, tongue few, thready pulse Number or band string.

Premature ovarian failure accounts for the 1%～3.8% of women's total number of persons, is the commonly encountered diseases in gynecological endocrine field.Owing to this type of is suffered from Person shows as early sending out or primary amenorrhea, estrogen are lowly caused perimenopausal symptom and infertile, therefore belong to traditional Chinese medical science amenorrhea, early send out absolutely Warp, menopausal syndrome, the category such as infertile.Owing to suffering from a deficiency of the kidney, function senilism is appointed in punching, and the dyspoiesis of menses causes uterus vessels of the uterus (blood Sea) hollow, depletion of blood can descend and menopause praecox."Fu Qingzhu works on obstetric" cloud: " the most disconnected through water, it appears that kidney water declines and dries up ".Because women is with blood It is main, and " YIN often being in shortage, YANG often being in excess ", therefore treatment should focus on tonifying kidney and nourishing yin, promoting blood flow to regulate menstruation.In tonifying kidney and nourishing yin Fang Fang, Radix Rehmanniae Preparata is grown Kidney yin, benefit marrow is monarch drug.Premature ovarian failure has obvious heterogeneity to clinical manifestation from the cause of disease to the course of disease, carry out correct Etiological diagnosis, dialectical combines with differential diagnosis of diseases, takes corresponding treatment strategies, to improve healthy or to delay gonad old and feeble.

1. amenorrhea is divided into primary amenorrhea and secondary amenorrhea, and secondary amenorrhea occurred before 40 years old.By the POF to large sample The investigation of patient does not has the tendency of distinctive irregular menstruation before finding amenorrhea.Somebody is unexpected after the menstruation of rule Amenorrhea, has plenty of and stops amenorrhea after contraceptive or childbirth, the disorder then showing as menstrual cycle and menstrual period before amenorrhea having.

The most infertile some patients finds premature ovarian failure because of infertile medical.Infertile is that chain reaction in patients with premature ovarian is medical and worried Main cause.There are fertility and infertility, so suggestion has premature ovarian failure family history person should plan pregnancy as early as possible.

3. low estrogen symptom primary amenorrhea person low estrogen symptom (hectic fever and/or dyspareunia etc.) is rare (22.2%), also mostly using estrogen replacement therapy relevant with the past if had, secondary amenorrhea person's low estrogen symptom is common (85.6%).This is that withdrawn the theory caused by estrogen consistent with low estrogen symptom.These low estrogen symptoms are also wrapped Include atrophic vaginitis and the atrophic urethritis such as frequent micturition, dysurea.

4. the performance of the autoimmune disease occurred together such as Addison disease, thyroid disease, diabetes, lupus erythematosus, class Rheumatic arthritis, vitiligo and crohn etc..Additionally also has the concealment symptom of adrenal insufficiency, such as subtracting of recent body weight Gently, loss of appetite, indefinite abdominal pain, weak, cutaneous pigmentation increase the weight of and addicted to salt.

Tonifying kidney and nourishing yin of the present invention, promoting blood flow to regulate menstruation.Radix Rehmanniae Preparata nourishing kidney-yin in tonifying kidney and nourishing yin side, benefit marrow is monarch drug.Fructus Corni, what The Radix Polygoni Multiflori, Semen Cuscutae liver and kidney tonifying, support essence and blood.Radix Arnebiae (Radix Lithospermi), Radix Paeoniae Rubra activating blood circulation to dissipate blood stasis, promoting the circulation of blood nourishing the liver blood, adjust punching to appoint.Herba Epimedii tonification liver Kidney.Fructus Ligustri Lucidi, Herba Ecliptae are ERZHI WAN, effect tonifying kidney and nourishing liver benefit essence.Radix Bupleuri nourishing YIN and clearing away heat pathogenic fire purging.Radix Puerariae clearing away heat and promoting production of body fluid, according to existing For pharmacological research Radix Puerariae, there is estrogen-like effects, for women's reproductive endocrine, there is regulation effect.All medicines share plays benefit altogether Kidney nourishing yin and activating blood, takes good care of punching and appoints effect.Invigorating kidney for regulating menstruation Chinese medicine has delay adenasthenia effect, has again promotion ovarian function Restitution, kidney-nourishing tcm drug has certain regulation effect to female gonad s function simultaneously, finds to mend by animal experiment study Renal drug has promotion ovary LH/HCG receptor to increase phenomenon.Chinese medicine there may also be promote fsh receptor produce effect, nourishing the liver and kidney, Benefit intensive culture blood Chinese medicine has the effect promoting follicular development.

Caulis Spatholobi is bitter, sweet, temperature.Return liver, kidney channel.Cure mainly: relaxing muscles and tendons to promote blood circulation；Nourishing blood for regulating menstruation.Main numb hand and foot；Quadriplegia； Rheumatic arthralgia；Women has through uncomfortable；Dysmenorrhea；Amenorrhea.Function: enrich blood, invigorates blood circulation, dredging collateral.For menoxenia, blood deficiency and yellow complexion, fiber crops Wood paralysis, rheumatic arthralgia." decoction pieces is newly joined ": remove blood stasis, tissue regeneration promoting blood, fluent passages through which vital energy circulates.Control summer filthy infection, wind arthralgia due to stagnation of blood disease." in modern practicality Medicine ": for the hematonic of robustness, it is adaptable to anemicus neural paralysis disease, such as limbs and soreness of waist and knee joint, apathy etc..Again For menoxenia, menstruation apolipsis etc., there is the effect of invigorating blood circulation analgesia.

Fructus Corni, cures mainly pathogen cold and heat under the heart, warming middle-JIAO, by cold temperature numbness, goes three worms, and clothes have been made light of one's life by commiting suicide for a long time, and reinforcing YIN-essence benefit is smart, pacify five The merit of nine orifices, only dribbling urination dirty, logical；Take improving eyesight, strength for a long time all the year round.Fructus Corni, has another name called Fructus Corni, medicine Fructus Jujubae, real Fructus Jujubae, Fructus Jujubae Skin, meat Fructus Jujubae etc., commonly use rare Chinese medicine for China, and applicating history is long.It is gentle with its benefit power, tonifying YANG and do not help fire, YIN nourishing And oiliness diaphragm, convergence and do not stay the special efficacies such as heresy to be liked use by successive dynasties medical science.Zhang Zhongjing has been formulated " golden deficient with Fructus Corni for monarch Shenqi pill ".According to chemical analysis, Fructus Corni contains the stronger cornin of physiologically active, tartaric acid, gallic acid, malic acid, tree The effective ingredient such as ester, tannin and multivitamin, have enhancing immunity, antiinflammatory, the pharmacological action such as antibacterial, are normal in tcm clinical practice Herba indigoferae Pseudotinctoriae.

Radix Bupleuri meridian distribution of property and flavor: cold nature, bitter in the mouth, pungent, return Liver Channel, gallbladder meridian, tool depressed liver-energy dispersing and function of gallbladder promoting, dredge gas resolving depression, dissipate the merit of fire Effect.Function: expelling pathogenic factors from the exterior expels the heat-evil, dispersing the stagnated live-QI to relieve the stagnation of QI, rise and lift yang-energy.Cure mainly: the heating that cures cold, cold and heat deficient fever.Children's's varioliform exanthema waste heat, Emaciation and sallow complexion abdominal part expands, [3] malaria, stagnation of QI due to depression of the liver, sternal rib pain, and proctoptosis, uterus comes off, menoxenia." book on Chinese herbal medicine is just ": " Radix Bupleuri, cool scattered with it with this person, the heat of suppressing the hyperactive liver.It is cool in nature, therefore solves alternate attack of chill and fever, flesh table hectic fever, and liver and gall fire is scorching, and chest and hypochondrium is tied, Double skin infection of controlling, blood room is heated；Its property dissipates, therefore main typhoid fever pathogenic heat do not solve, epidemic febrile disease intenseness of heat, SHAO YANG headache, Liver Channel melancholia.

Fructus Ligustri Lucidi, sweet-bitter flavor, cool in nature, return Liver and kidney warp, there is nourishing the liver and kidney, merit that tonifying YIN nourishes blood.Modern medicine study is demonstrate,proved Bright, Fructus Ligustri Lucidi includes ursolic acid, mannitol, glucose, fatty oil etc., has heart tonifying, diuresis and hepatoprotective effect, can make because chemotherapy is drawn The leukopenia quantity risen raises, and staphylococcus aureus, escherichia coli are had inhibitory action.Containing neat pier in Fructus Ligustri Lucidi The acid of fruit acid Fructus Ligustri Lucidi, nuezhenoside, ursolic acid, stearic acid, oleic acid, mannitol, betulin, phospholipid and saccharide such as Fructus Ligustri Lucidi are many Sugar, arabinose, glucose etc., possibly together with 15 kinds of aminoacid, 11 kinds of trace element and volatile oil etc..Research thinks that Fructus Ligustri Lucidi has Blood sugar lowering and blood fat reducing also have study of anti-atherogenic effect, the most antibacterial, antivirus action, and Fructus Ligustri Lucidi decoct is to golden yellow Portugal Grape coccus, shigella flexneri, Bacillus typhi, bacillus pyocyaneus and escherichia coli all have inhibitory action.Type B adenovirus is had medium Degree inhibitory action.Leukocyte increasing: Fructus Ligustri Lucidi and component preparation thereof all have certain impact to hemocyte, it is to white caused by chemotherapy Cell declines rising effect.Compound recipe containing Fructus Ligustri Lucidi also has effect in increasing leukocyte.Anti thrombotic action: oleanolic acid can accelerate blood The mobility of platelet cell, reduces the collision between platelet, is allowed to be difficult to adhesion and gathering, is less susceptible to sink in tunica intima Long-pending, slow down or prevent thrombosis, the deposition of lipid inner membrance can be reduced again, the thrombotic disease for preventing and treating old people provides portion Divide experimental basis.Defying age improves immunity: Fructus Ligustri Lucidi decoct can make mouse immune organ thymus, spleen weight increase；Substantially Improving mice serum hemolytic antibody activity, the impact that mouse spleen lymphocyte produces interleukin-2 has two-ways regulation work With.Fructus Ligustri Lucidi has significant immunological enhancement, removes free radical thus defying age.Application Fructus Ligustri Lucidi preventing and treating: cardiovascular disease Disease, coronary heart disease, hyperlipemia, diabetes, thrombocytopenia, leukopenia, chronic nephritis, chronic bronchitis, stupid Solidity insomnia, Chronic Benzene Poisoning, tumor, malnutrition, immunologic hypofunction disease, seborrheic dermatitis

Cortex Moutan, Chinese medicine name.It is dried root bark for ranunculaceae peony.Originate in the ground such as Anhui, Shandong.Autumn excavates root, Remove radicula, strip root bark, dry.Life is used or is fried and uses.Compendium of Material Medica: " nourishing YIN to lower pathogenic fire solves speckle poison, throat, diuresis blood Stagnant.Descendant is specially to control fire mutually with yellow tiller, does not knows that the merit of Cortex Moutan more wins also.Red colored person profit, the person of spending in vain mends, and people the most seldom realizes, and preferably distinguishes It." modern study, contained paeonol and glucosides constituents in addition thereof all have antiinflammatory action；The methanolic extract of Cortex Moutan has Suppression Platelet；Paeonol has the central inhibitory actions such as calmness, cooling, antipyretic, analgesia, spasmolytic and anti-atherogenic hard Change, diuresis, antiulcer etc. act on.

Radix Salviae Miltiorrhizae is bitter, is slightly cold.GUIXIN, Liver Channel.Stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, clear away heart-fire relieving restlessness.For menoxenia, amenorrhea pain Warp, lump in the abdomen, chest and abdomen twinge, pyretic arthralgia pain, skin infection swells and ache, dysphoria and insomnia.For costa sternales hypochondriac pain, rheumatic arthralgia, lump in the abdomen is tied Block, skin infection swells and ache, and tumbling down is grieved, menoxenia, amenorrhea dysmenorrhea, puerperal stasis of blood pain etc..Treatment costa sternales pain, lump in the abdomen caking, and Menoxenia, amenorrhea dysmenorrhoea have good effect." detailed outline " carries: Radix Salviae Miltiorrhizae, and by " the sensible opinion of married woman " cloud, SIWU TANG is controlled gynecopathy, paid no attention to Antenatal puerperal, how many through water, the most generally applicable, only taste Radix Salviae Miltiorrhizae dissipates, and cures mainly same.Lid Radix Salviae Miltiorrhizae can break stagnated blood, mends fresh blood, peace Green tire, fall stillborn fetus, only metrorrhagia dysentery, regulating menstruation arteries and veins.

Fructus Rubi plant can be used as medicine, and has multi-medicament to be worth, and its fruit has the effect of kidney invigorating and YANG supporting.Raspberry seed oil belongs to not Satisfied fatty acid, can promote that the severe that of prostatic secretions covers.Effect: the liver and the kidney tonifying, controlling nocturnal emission with astringent drugs reducing urination, improving eyesight.With rat, the vagina of rabbit Treatment index is made in smear and inner membrance section, and Fructus Rubi seemingly has estrogen-like effects.Bacteriostasis 100% decoct flat board burrows Method, has inhibitory action to staphylococcus, also has inhibitory action to vibrio cholera.Estrogen-like effects rat, the vaginal smear of rabbit And the index such as inner membrance section shows, Fructus Rubi seemingly has estrogen-like effects.

Herba Leonuri acrid in the mouth is bitter, cool.Invigorate blood circulation, silt of dispelling, regulating menstruation, disappear water.Treatment menoxenia, vaginal bleeding during pregnancy difficult labour, afterbirth is not Under, puerperal blood faints, abdominal pain due to blood stasis, metrorrhagia and metrostaxis, hematuria, rushes down blood, carbuncle skin infection.Herba Leonuri alkalescence subcutaneous injection has CNS inhibition Effect, MLD is 0.4-0.6g/kg.Intravenous rabbit injection leonurine 1mg/kg, it is seen that urine volume dramatically increases.Leonurine is relatively Sanguis Leporis seu oryctolagi suspension generation haemolysis can be made during high concentration.Leonurine infusion tube dilution method 1: 13-1: 10, to Lan Shi perhaps Trichobacteria, Pilus Caprae seu Ovis sample sporidiole bacteria, epidermophyton rubrum, starlike nocadia all have inhibitory action.Herba Leonuri decoct sheet paper Sheet method, has inhibitory action to escherichia coli, dysentery bacterium.Herba Leonuri can remove silt tissue regeneration promoting, and promoting blood flow to regulate menstruation, containing selenium, manganese Etc. various trace elements, can antioxidation, anti-aging, there is pretty good beneficial face beauty treatment, anti-aging effect.

Radix Puerariae, Chinese medicine name.For the dry root of legume pueraria lobata, practise and claim Herba Gelsemii Elegantis.Autumn, season in winter two excavate, and take advantage of fresh-cut and become thickness Sheet or fritter；It is dried.Sweet, pungent, cool.There are expelling pathogenic factors from muscles for reducing heat, rash, promoting the production of body fluid to quench thirst, the merit of yang invigorating antidiarrheal.It is usually used in exterior syndrome heating, Stiff nape and back, measles without adequate eruption, calentura is thirsty, and the deficiency of YIN is quenched one's thirst, diarrhea of heat type hematodiarrhoea, diarrhea due to hypofunction of the spleen.

Semen Cuscutae: Cuscuta chinensis is dicotyledon scammonia section plant Semen Cuscutae, Cuscuta australis, cuscuta japonica Choisy etc. Seed, can the kidney invigorating and essence nourishing, nourishing the liver to improve visual acuity, effect of solid tire stopping leak.Nourishing the liver and kidney, controlling nocturnal emission with astringent drugs reducing urination, antiabortive, improving eyesight, antidiarrheal.For Impotence and seminal emission, dripping urination, enuresis frequent micturition, soreness of the waist and knees, blurred vision tinnitus, vaginal bleeding due to deficiency of the kidney during pregnancy, frequent fetal movement, spleen kidney vaginal bleeding during pregnancy, fetal movement Uneasy, spleen is suffered from a deficiency of the kidney and is rushed down；Externally treating leucoderma.Former medicine sieve is removed spare, picks decontamination, dry.

Radix Rehmanniae Preparata is the tuber of scrophulariaceae rehmannia glutinosa plant (formal name used at school: Rehmannia glutinosa), has another name called Radix Rehmanniae Preparata or volt Ground, the processed process of preparing Chinese medicine forms.Generally with wine, Fructus Amomi, Pericarpium Citri Reticulatae for adjuvant through repeatedly steaming solarization, glossy to inside and outside darkish complexion, quality is soft Sticky.Section use, or parch to black use.Radix Rehmanniae Preparata has another name called Radix Rehmanniae Preparata or throws oneself on the ground, and belongs to goatweed, is a kind of first-class Chinese crude drug, has YIN nourishing of enriching blood effect, can be used for blood deficiency and yellow complexion, dizziness, palpitation and insomnia, menoxenia, the disease such as metrorrhagia, also can be used for deficiency of kidney yin Hectic fever hectic fever due to YIN-deficiency, night sweat, pass out semen, the disease such as quench one's thirst, be one of deficiency syndrome class nonprescription drugs medicine LIUWEI DIHUANG WAN main component.

Fructus Schisandrae Chinensis is magnoliaceae schisandra Schisandra chinensis (Turcz.) Baill. or Central China five tastes The dry mature fruit of sub-Schisandra sphenanthera Rehd.et Wils..The former practises title " Radix Schisandrae Bicoloris ", the latter Practise and claim " Fructus Schisandrae Sphenantherae ".Pluck during fruit maturation in autumn, dry after drying or steaming, remove carpopodium and impurity." the Xin Xiuben such as Tang Grass " carry " the sweet acid of five tastes skin and flesh, arduous in core, have saline taste ", therefore have the name of Fructus Schisandrae Chinensis.Fructus Schisandrae Chinensis is divided into two kinds of south, north.Ancient doctor Book claim it, profound and, meeting and, be listed in the top grade Chinese medicine in Sheng Nong's herbal classic the earliest, can the power of strengthening by means of tonics, medical value is high, There is the effect of building body, share treatment insomnia with fine jade treasure Ganoderma.

Herba Ecliptae grass is the conventional liver-nourishing kidney-boosting of the traditional Chinese medical science, cooling blood for hemostasis medicine.To the Light-headedness caused by the hepatic and renal YIN deficiency, tooth Loose compact, aching pain in waist and back, all diseases of flaccidity and weakness of the lower limbs and caused by heat in blood multiple go out blood disorder have good efficacy.Herba Ecliptae contains volatilization The chemical compositions such as oil, tannin, Saponin, Herba Ecliptae element and vitamin A.Animal experiment has haemostatic effect, and in vitro tests is to golden yellow Staphylococcus has inhibitory action.

Herba Epimedii is the multiple Herba Epimedii such as Berberidaceae plant Herba Epimedii, Epimedium sagittatum, Epimedium wushanense, Herba Epimedii Stem and leaf.Taste is returned through acrid, sweet, warm.Liver, kidney channel is returned to cure mainly function: kidney-replenishing, bone and muscle strengthening, wind-damp dispelling.Herba Epimedii has and prolongs Slow old and feeble, blood pressure lowering, blood sugar lowering, blood fat reducing.Improve cerebral hypoxia ischemia, reduce blood viscosity, suppression ex vivo thrombosis, stimulation The synthesis of bone marrow DNA, increase peripheral blood leucocyte and the quantity of marrow hemopoietic stem cells, improve immunity, promote bone growth, Pre-preventing bone rarefaction, antiinflammatory, antiviral, antitumor, the side effect of antagonism cancer therapy drug.

Chinese medicine Radix Polygoni Multiflori have Radix Polygoni Multiflori and Radix Polygoni Multiflori Preparata point, direct slicing is used as medicine for Radix Polygoni Multiflori, after mixing steaming with Semen sojae atricolor liquor Dry and be used as medicine for Radix Polygoni Multiflori Preparata.The function of the two is different: the removing toxic substances of Radix Polygoni Multiflori function, eliminating carbuncle, loosening bowel to relieve constipation, is usually used in treating scrofula Scrofula carbuncle sore, rubella pruritus, dryness of the intestine constipation；Radix Polygoni Multiflori Preparata function invigorating the liver and kidney, benefiting essence-blood, crow beard and hair, bone and muscle strengthening, for blood deficiency and yellow complexion, Vertigo and tinnitus, early whitening of beard and hair, soreness of the waist and knees, numb limbs and tense tendons, bleeding not during menses, chronic malaria body are empty.Radix Polygoni Multiflori Preparata of the present invention It it is the good merchantable brand of invigrating kidney.

Radix Paeoniae Rubra, Chinese medicine name.For ranunculaceae plant Radix Paeoniae Rubra or the dry root of river Radix Paeoniae Rubra.Spring, season in autumn two excavate, removing rhizome, Fibrous root and silt, dry.Hardship, is slightly cold.Return Liver Channel.There is clearing away heat and cooling blood, effect of blood circulation promoting and blood stasis dispelling.Radix Paeoniae Rubra is in famous wild tunnel Medical material,

External:

Rhizoma Smilacis Glabrae is sweet light, flat.Gui Jing: Liver Channel；Stomach warp；Spleen channel.Function: removing toxic substances, dehumidifying, profit joint.Cure mainly: control syphilis, Stranguria with turbid discharge, muscles and bones spasm and pain, tinea pedis, treat furuncle, carbuncle, scrofula, the limbs contracture caused by syphilis and mercurialism, bones and muscles pain." book on Chinese herbal medicine Justice ": Rhizoma Smilacis Glabrae, dampness removing reduces phlegm and internal heat, and can enter network, searches to pick and damp and hot accumulates poison.It solves d hydrargyrum, Calomelas poison person, and that are received on poison with ascending OK, and this leads as business to ooze under profit, therefore specially controls Fructus Myricae rubrae skin infection, gos deep into hundred networks, and arthralgia is the most rotten, and poison fire is up again, pharynx Laryngalgia is burst, and all dislike disease.

Fructus Cnidii is pungent, bitter, temperature；Slightly poisonous.Return kidney channel.Cure mainly warming the kidney to invigorate YANG, dampness, dispel the wind, parasite killing.For sexual impotence, Cold womb, cold-damp leukorrhagia, arthralgia chiefly caused by damp pathogen lumbago；External treatment vulval eczema, married woman's pudendal pruritus；Trichomonal vaginitis, is gynecological external use good medicine." this Grass is through dredging " carry: Fructus Cnidii, bitter in the mouth is put down；" not Lu " be pungent sweet nontoxic；Modern its abnormal smells from the patient in detail, when warm-dryness syndrome of must holding concurrently, sun is also.Therefore housewife people is cloudy In swell and ache, man's impotence pruritus due to damp pathogen, eliminating impediment gas, profit joint, malignant boil." not Lu " warming the middle warmer and descending qi, makes married woman's clothes heat, and man is cloudy strong, Make us there is son.Lid can dehumidify with tongue, temperature energy cold expelling, the pungent kidney that can moisten, sweet energy strengthening the spleen, therefore can be given birth to except all deficiency and coldness of married woman man are wet Sick.Cold-damp was both removed, then disease is gone, performance Yiyang, thus can disease, and have tonification also.

Radix Sophorae Flavescentis is bitter, cold.GUIXIN, liver, stomach, large intestine, bladder are through heat clearing and damp drying, parasite killing, diuresis.For hematodiarrhoea, have blood in stool, yellow Subcutaneous ulcer urine retention, leucorrhea with red and white discharge, swelling of the vulva pudendal pruritus, eczema, eczema, skin pruritus, scabies leprosy；External treatment colpitis mycotica, Chang Yuhuang Cypress is used in combination, and is to treat colpitic external use gynaecological good medicine.

Folium Artemisiae Argyi is pungent, bitter, temperature, nontoxic；Return liver,spleen,kidney warp.Dispersing cold for relieving pain, warming the meridian for stopping bleeding.For few abdomen cold type of pain, through trembling with fear not Adjust, cold womb is infertile, spit blood, epistaxis, metrorrhagia through many, gestation hemorrhage；External treatment skin pruritus, decortication.Regulate the flow of vital energy blood, by cold-damp；Warming the meridian, Hemostasis, antiabortive.Control trusted subordinate's cold type of pain, convulsion of having loose bowels, chronic dysentery, tell nosebleed, hematochezia, menoxenia, metrorrhagia, leukorrhagia, frequent fetal movement, carbuncle Infections, scabies." not Lu ": main moxibustion all kinds of diseases and ailments.Can decoct, only dysentery, spit blood, hideing skin ulcer in bottom, married woman leaks blood.Li Yin gas, granulation promoting meat, ward off Wind and cold, makes people have son.TAO Hong-Jing: smash leaf with moxibustion all kinds of diseases and ailments, the most only blood trouble.

Indigo Naturalis nature and flavor: salty, cold.Gui Jing: enter Liver Channel, lung meridian, stomach warp.Function: heat clearing away, blood cooling and ecchymoses removing, removing toxic substances, liver heat removing is rushed down Fire, arresting convulsion.Curing mainly: control epidemic febrile disease intenseness of heat, macule, spit blood, spit blood, pediatric epilepsy scared, skin ulcer swells, erysipelas, snake bite and insect sting." book on Chinese herbal medicine warp Dredge ": " Indigo Naturalis, detoxify heat extraction, Gu its chief, more ancient prescription has and be used for all blood disorder persons, makes non-blood system excess-heat, and diease occurrence is in the deficiency of YIN Interior-heat, YANG unable to attach to somewhere, fire of moxibustion, because of scorching in void, send out and coughs up saliva etc. and demonstrate,prove for telling nosebleed, with non-preferably.Blood must be trembled with fear, and coagulates, and solidifying then cold and heat are handed over and made, Chest and diaphragm or pain, more increase its disease." " herbal classic is met former ": " Indigo Naturalis, eliminating pathogen in the liver gallbladder dissipate stagnated fire, control maculae caused by violent heat pathogen and puerperal weighs under hematodiarrhoea, " a thousand pieces of gold " blue blue or green ball with it, the headache of sky row cold and heat, water grinds and takes it.Similar with indigo plant, and the merit of parasite killing of drawing out pus by applying a plaster to the affected part of stopping blooding, seemingly it is better than indigo plant. Control again dysphagic disease, take its power changing worm also.With white, Borneolum Syntheticum of drowning, blowing sheet, mouthpiece infantile malnutrition is imitated most.”

Borneolum Syntheticum acrid in the mouth, hardship, be slightly cold；GUIXIN, liver, lung meridian；Delicate fragrance a surname dissipate, and has refreshment of having one's ideas straightened out, and heat clearing away dissipates poison, improving acuity of vision and removing nebula Effect, cure mainly calentura unconsciousness due to high fever, apoplexy syncope due to accumulation of phlegm infantile convulsion, heat-damp in summer blinds clear key, and sore throat is deaf, and aphtha tooth swells, carbuncle sore infantile malnutrition hemorrhoid, Conjunctival congestion and swelling pain, pterygium.Logical all keys, dissipate stagnated fire, removing nebula improving eyesight, reducing swelling and alleviating pain, and heat clearing away dissipates poison, dissipates fire removing toxic substances.Control mammary gland knot Block, apoplexy locked mouth, calentura coma, infantile convulsion expectorant fan, deafness with qi stagnation, sore throat, aphtha, otitis media, carbuncle, hemorrhoid, mesh also nebula film, pinworm Parasitosis." not Lu ": " married woman has difficult labour, and takes Borneolum Syntheticum and grinds into powder a little, is taken after mixing with liquid newly to draw water." Tang Materia Medica: " main trusted subordinate pathogen, rheumatism Accumulation, deaf.Improving eyesight, goes conjunctival congestion skin nebula.External oculopathy eye in " " Haiyao Bencao, Oversea Materia Medica ": " is main, three worms, control five hemorrhoid, improving eyesight, relieving palpitation, secret Essence.″

Pseudobulbus Bletillae (Rhizoma Bletillae) property is bitter, sweet, puckery, is slightly cold.Return lung, liver, stomach warp.Function cures mainly astringing to arrest bleeding, detumescence and promoting granulation.Tell for hemoptysis Blood, traumatic hemorrhage, sore swollen toxin, chapped skin；Pulmonary tuberculosis hemoptysis, ulcer haemorrhage.Shennong's Herbal: " main carbuncle, evil Skin ulcer, lose cellulitis, the dead flesh of impairment of YIN, pathogen in stomach "；Compendium of Material Medica: " abnormal smells from the patient (root) is bitter, flat, nontoxic.

Radix Arnebiae (Radix Lithospermi) nature and flavor: bitter, cold, Gui Jing: enter percardium, Liver Channel.Function cures mainly: removing heat from blood, invigorates blood circulation, heat clearing away, removing toxic substances.Control warm Macule, jaundice due to damp-heat, purpura, tell, be defeated in battle, hematuria, stranguria with turbid discharge, constipation with heat retention, burn, eczema, erysipelas, carbuncle and ulcer." herbal classic ": " the main heart Abdomen pathogen, five types of jaundice, invigorating the spleen and replenishing QI, profit nine orifices, waterway.″

Herba Euphrasiae Regelii, formal name used at school: Euphrasia pectinata Ten, for annual herb plant, herb tool medical value. Herba Euphrasiae Regelii extract is the aerial parts of the herb of Herba Euphrasiae Regelii, has eye pouch, the effect of the fatigue that disappears.Bitter in the mouth；Cold nature, returns Warp, returns bladder warp, and function cures mainly heat-clearing and toxic substances removing；Diuresis.Main calentura is thirsty；Headache；Cough due to lung-heat；Laryngopharynx swelling and pain；Pyretic stranguria；Urine Unfavorable；Aphtha；Carbuncle.

Spica Prunellae is pungent, bitter, cold.Return liver, gallbladder meridian.Effect: clearing away heat-fire, improving eyesight, mass dissipating and swelling eliminating.Conjunctival congestion and swelling pain, have a headache dizzy Dizzy, mesh pearl nyctalgia.This product bitter cold master enters Liver Channel, and kind removing fire from the liver is with improving eyesight.With controlling flaming up of liver-fire, conjunctival congestion and swelling pain, Folium Mori, chrysanthemum can be joined Flower, Semen Cassiae etc. are medicinal.Among this product liver heat removing and eyesight improving, nourishing the liver of slightly holding concurrently, to join Radix Angelicae Sinensis, Fructus Lycii, can be used for deficiecny of liver-YIN, mesh pearl aches Bitterly, to night more particularly；Also Rhizoma Cyperi, Radix Glycyrrhizae use can be joined, as Spica Prunellae dissipates (" Zhang Shi doctor is logical ").Scrofula, goiter.This product acrid in the mouth can dissipate Knot, bitter cold can expel the heat-evil, and it is medicinal to control pathogenic fire derived from stagnation of liver-QI often to join Bulbus Fritillariae Uninbracteatae, Rhizoma Cyperi etc., and the scrofula of phlegm-fire cohesion, such as Spica Prunellae soup (" surgery Authentic ")；With controlling goiter, the most often join the use such as Thallus Laminariae (Thallus Eckloniae), Radix Scrophulariae, such as Spica Prunellae cream (Golden Mirror of Medicine).

The preparation process of oral pills of the present invention is: take Radix Paeoniae Rubra 1500g, Caulis Spatholobi 1500g, Fructus Corni 1500g, Herba Leonuri 1500g, Radix Bupleuri 1500g, Radix Arnebiae (Radix Lithospermi) 1500g, Radix Rehmanniae Preparata 1500g, Fructus Ligustri Lucidi 1500g, Herba Ecliptae 1500g, Fructus Schisandrae Chinensis 1500g, cover basin Sub-1500g, Semen Cuscutae 1500g, Herba Epimedii 1500g, Cortex Moutan 1500g, Radix Puerariae 1500g, soak 30 minutes to 1 hour, add Heat is boiled 30～40 minutes, filters, and filtrate is standby；Filtering residue adds water, second time heating, boils 30～40 minutes, filters, and filtrate is standby With；Filtering residue adds water, for the third time heated and boiled 30～40 minutes, filters；Three filtrates being combined, heating is concentrated into cream Shape, after oven drying, becomes fine powder, mixes Mel and be twisted into pill.

The manufacturing process of oral capsule of the present invention is: take Radix Paeoniae Rubra 1200g, Caulis Spatholobi 1100g, Fructus Corni 1100g, Herba Leonuri Grass 1200g, Radix Bupleuri 1100g, Radix Arnebiae (Radix Lithospermi) 1100g, Radix Rehmanniae Preparata 1200g, Fructus Ligustri Lucidi 1200g, Herba Ecliptae 1200g, Fructus Schisandrae Chinensis 1200g, cover Basin 1200g, Semen Cuscutae 1200g, Herba Epimedii 1200g, Cortex Moutan 1100g, Radix Puerariae 1200g, by raw material dry in the sun, be cut into coarse grain, so After be soaked in ethanol 2 hours.Then heating extraction 2 times, each 1-2 hour, united extraction liquid, removes supernatant, so after standing Rear 100-120 mesh filters, then the ultrafiltration post ultrafiltration that molecular weight is 5000-10000 through damming, ultrafiltrate concentrating under reduced pressure relative density The extractum of 1.36, stand for standby use when being 80 DEG C, become component 1.The medicinal residues of the crude drug crossed by above-mentioned ethanol extraction again add 10 times Amount water, heating and refluxing extraction 2 times, each 1～2 hour, 2 extracting solution are merged and stands, become component 2.By component 1 and component The merging of 2, is then placed in concentrating under reduced pressure tank, decompression recycling ethanol, and being concentrated into liquor strength is 0.6g crude drug/mL, and sucking filtration is extremely When the relative density of filtrate is 20 DEG C 1.06；Above-mentioned filtrate is after the macroporous adsorptive resins absorption that volume is 10L, with 10 times of trees The deionized water of fat column volume or distilled water eluting, then with 95% ethanol elution of 5 times of resin column volumes, collect ethanol elution Liquid, removes solvent, obtains medicated powder and load capsule after drying.

The preparation method of suppository of the present invention: Pseudobulbus Bletillae (Rhizoma Bletillae) 1000g, Indigo Naturalis 1000g, the Borneolum Syntheticum 1000g way of distillation are vaporized into knot Crystalline substance, then pulverizes, and crosses 100-120 mesh sieve, standby as component 1；Again by remaining crude drug Radix Arnebiae (Radix Lithospermi) 1000g, Radix Sophorae Flavescentis 1000g, Serpentis Machine tool 1000g, Rhizoma Smilacis Glabrae 1000g, Folium Artemisiae Argyi 1000g, Spica Prunellae 1000g, Herba Euphrasiae Regelii 1000g shred, and are soaked and have been placed in lid In stainless steel cask, adding 70% ethanol, quality is coarse grained 0.8-1 times, stirs, moistening airtight placement more than 1 hour, Make fully to expand；After filter plate gauze bag parcel is paved bottom percolator, the drug sample after moistening expansion is mixed pine lane again Dissipate, then contain powder with rustless steel spoon, load percolator uniformly, fill 10-12 cm thick, even by T-bar pressure, then by above-mentioned behaviour Making, loading in layer, suitably pressurize, medicated powder loads 2/3 eminence that must not exceed percolator；Upper cover rustless steel hole, medicated powder face Plate fastens, and opens the baiting valve below percolator, and puts a container, is then slowly added into 70% ethanol；Medicated powder powder to be discharged Air between Li, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open material Valve carries out percolation, controls percolation speed and is generally 1000g medical material outflow per minute 2～3ml, and filtrate puts into liquid container, and will Ethanol during emptying pours liquid container into；Percolate is merged and stands, as component 2；Component 1 component 2 is mixed into ointment；Choose 50 DEG C～60 DEG C heating and melting of substrate, add ointment cooling and shaping obtained above and make class warhead shape suppository, and ultraviolet is killed virus. Substrate used by described suppository is selected from: one in semi-synthetic fatty acid glyceride, poloxamer, Polyethylene Glycol, lanoline or Multiple；Described substrate is heating and melting under the conditions of temperature 50 C～60 DEG C, adds the medicine mastic that described preparation method prepares, water Bath stirs, and is mixed into the liquid of thickness, is poured in plastic shape-forming pipe, inserts tampon, and cooling and shaping makes bullet-shaped Suppository；Described tampon is the afterbody tampon with cotton thread.

The detailed directions of described suppository is as follows: drug suppository of the present invention is removed cabinet, takes out stype, pulls out afterbody Cotton thread, includes in stype bottom vagina, and the most once, cotton thread is stayed external, plays pull-out tampon morning next day, and continuous use 7 days is One course for the treatment of.

Toxicology test:

Test example 1: acute toxicity test of the present invention

One, test material:

Animal: Kunming mouse, body weight 21-24g, male and female half and half, Shandong University's biology laboratory breeding.Medicine: this Bright oral capsule.

Two, method:

1, LD50 calculates: using improvement karber's method, mice is randomly divided into 5 groups, often group 10, male and female half and half, by suppository Dissolving, be made into Cmax, be administered by the maximum allowable capacity of mice, institute is followed successively by 18 to dosage by crude drug amount, and 14.4,11.5, 9.2,7.4 (g.kg-1), after animal fasting (can't help water) 18 hours, are administered in one day (interval half an hour), every time at twice 0.5ml, treats animal dead situation.

2, maximum tolerated dose measures (MTD value): take mice 20, each 10 of male and female.Suppository adds distilled water dissolve, join Become maximum concentration, by the maximum tolerated dose of animal, can twitch with injection filling hello device and be as the criterion.Animal fasting (can't help water) 18 hours After, be administered (interval half an hour) at twice in one day, each 0.5ml (every ml 0.36g Han crude drug), total dose be 18g crude drug/ Kg.d, 300 times of quite clinical adult's 50Kg body weight consumption.Treat 7 days continuously after administration.

Three, result of the test:

In LD50 calculates when giving mice with maximum acceptable concentration and maximum allowable capacity (18g/Kg.d), have no little Mus is dead, does not i.e. measure LD50, seeks maximum tolerated dose only, interim treatment in 7 days, its appetite of animal, activity, hair color, essence God's states etc. are all normal, grow normal, there are no death.I.e. select and be equivalent to 300 times of doses of clinical dosage, there is no bad instead Should occur, show that acute toxicity is minimum, MTD ＞ 18g/Kg.d.

Test example 2: acute skin irritation test of the present invention is reported

1, experiment purpose: detect suppository of the present invention to the stimulation/corrosiveness of laboratory animal skin and intensity

2, material and method:

2.1. tested material: title: suppository of the present invention (embodiment 3 method prepares)

2.2. laboratory animal: one grade of big ear white race rabbit, 10, female, body weight 2.1-2.3kg；By University Of Qingdao's medical science Biology department of institute Experimental Animal Center provides；

2.3. test method:

Select the healthy rabbits 10 that skin is intact, its spinal column both sides, back were cut by hair in first 24 hours in test, go The left and right each about 4cm × 4cm of gross area.Take tested material 0.2ml during test to be applied on two layers of gauze of 2.5cm × 2.5cm, apply ointment or plaster In skin of unhairing surface, side, oilpaper covers, immobilization with adhesive tape.Opposite side skin is as blank.After closing 4 hours, remove Covering, and go to remain tested material with warm water cleaning, area skin is smeared in the treatments in 1,24 and 48 hours after removing tested material Reaction, and mark.

3, experimental result: any erythema does not occurs in laboratory animal skin, red and swollen, inflammation phenomenon.

4, brief summary: judge according to skin wound repair standards of grading in " disinfection technology standard " (1999), ointment of the present invention Agent belongs to nonirritant to animal skin stimulus intensity.

Embodiment 3: vaginal mucomembranous irritant test result

1. test method:

After laboratory animal is quarantined 3 days, select 18 above-mentioned healthy rabbits, be randomly divided into three groups, often organize each 6, be respectively as follows: Blank, excipient control, suppository group of the present invention (by application 5 times of concentration of liquid).Respectively with appropriately sized sterilizing cotton sliver leaching It is placed in excipient control group and tested material treated animal intravaginal with sterile saline and tested material, contacts 6 with vaginal mucosa little Time, put to death animal after 24 hours.Taking-up topical vaginal tissue, treats pathological changes situation.

2, result of the test: vaginal mucomembranous irritant test result

Embodiment 4: skin anaphylactic test result

Use suppository of the present invention, carry out inducing and exciting contact test, therapeutic test mice behind 24 hours and 48 hours, all Lose skin and have the phenomenon such as erythema and edema, with negative control group zero difference.Result shows, this suppository is near to dermoreaction intensity In zero, i.e. without skin allergic reaction.

Embodiment 5: long term toxicity test data

1, experimental technique

Random for Cavia porcellus part is become 4 groups, often group 15.In guinea pig back spinal column both sides, depilatory is uniformly coated with so that it is de- Hair scope about 40 square centimeters.Clean depilatory, often organize Cavia porcellus after treating 24 hours and be coated with suppository solution 0.2,0.4 of the present invention respectively And 0.8ml, respectively containing crude drug 92,184 and 368mg, another group is coated with the every twice-daily of solvent 0.8ml, continuous 30 days, treats Cavia porcellus Ordinary circumstance, experiment is put to death animal after terminating and is carried out hematology, blood biochemical and pathological examination.

2, result

Above-mentioned three groups of medication Cavia porcellus trunks go to hair-fields, have no that local skin has edema, hyperemia, erythema, petechia and ulcer. Medication group and control animals chroma of hair, ingest, matched group no significant difference, blood biochemistry checking, the medication such as extremity are movable Group and matched group are all in normal range.Histopathologic examination, tests each group of heart, liver, kidney and local skin and is showed no obvious disease Become.Prompting, Chinese medicine suppository long-term prescription of the present invention is showed no obvious toxic action to local skin and whole body important organ.

Result of the test shows: Chinese medicine suppository experiment safety of the present invention.This medicine to group B streptococcus, staphylococcus aureus, Colibacillary minimal inhibitory concentration is 1: 150, and gonococcus is 1: 150, and Candida albicans is 1: 300, during to 1: 20 dilution factor Time of killing be 5 minutes, be 10 minutes when 1: 50, be 20 minutes when 1: 100, be 40 minutes when 1: 200, be 8 little when 1: 400 Time.

Clinical trial:

From in November ,-2015 in January, 2014, we are from intending tonifying kidney and nourishing yin side's treatment premature ovarian failure 40 example, and curative effect is preferable, report Accuse as follows.

1 clinical data

Totally 80 example, secondary amenorrhea, FSH rising person before being 40 years old.Age 17～39 years old, average (33.3 ± 6.8) year； Amenorrhea 1～11 years, average (3.6 ± 3.7) year.There are hypomenorrhea, or irregular bleeding before amenorrhea, or take place frequently or menstrual blood volume Few, or amenorrhea suddenly.The wherein i.e. amenorrhea in 3 example menophania 1～3 years, has 2 examples to take traditional Chinese medicine Radix Tripterygii Wilfordii history, and 1 example is suffered from vitiligo and taken Western medicine (the most not quite clear)；Amenorrhea after 2～5 years, has 6 examples to have chronic pelvic inflammatory disease history.There is its mother amenorrhea before 40 years old of 3 examples.There are 3 example secondary Property is infertile, 1 example primary infertility.All there are hectic fever, perspiration, anxiety, depression, insomnia or hypomnesis, or vaginal dryness, sexual intercourse The symptoms such as pain or urine urgency-frequency.80 examples are randomly divided into Chinese drug-treated group and Western medicine group.

Western medicine diagnose standard is to occur amenorrhea in more than at least 4 months before 40 years old, and has 2 times or above serum FSH ＞ 40U/L (2 inspection intervals more than 1 month), estradiol level ＜ 73.2pmol/L.

Tcm diagnosis standard is cracked ends before 40 years old, infrequent menstruation, gradually to amenorrhea or menolipsis suddenly, warms sweating, hectic fever Flushing, dysphoria with feverish sensation in the chest palms and soles, dizziness and tinnitus, soreness of the waist and knees or metapedes are with pain, red dry stool of urinating, emaciated physique, vaginal dryness.B ultrasonic shows son Palace is less than normal or both sides ovary is less than normal, does not finds follicle or monitors the most developable little follicle of rarely seen only a few, serum E continuously₂ Low SI, FSH is significantly raised, red tongue or have crackle, tongue few, thready and rapid pulse or band string.

2 Therapeutic Method

Chinese drug-treated group tonifying kidney and nourishing yin side.Medicinal Radix Rehmanniae Preparata 12g, Radix Paeoniae Rubra 13g, Radix Arnebiae (Radix Lithospermi) 12g, Fructus Corni 11g, Fructus Schisandrae Chinensis 11g, bavin 12g, Fructus Ligustri Lucidi 13g, Herba Ecliptae 12g, Radix Polygoni Multiflori Preparata 12g, Rhizoma Curculiginis 13g, Herba Epimedii 12g, Semen Cuscutae 12g, Radix Puerariae 15g, Cortex Moutan recklessly 12g.Every day 1 dose, take day 2 times, within 3 months, be a course for the treatment of.

Western medicine group premarin, each 0.625mg, every day 1 time, it serve on 22 days；Within 18th day, add medroxyprogestetone acetate, every day 1 time, each 10mg, menstrual onset the 5th day or menstruation do not rise, and drug withdrawal is further continued for taking medicine on the 7th day.Serveing on 3 months is a treatment Journey.

All treat statistical result after 1 course for the treatment of for two groups.

3 observational techniques

Clinical symptoms and menstrual cycle before and after treatment, through the change of amount, the change of Serum Sex Hormones, ultrasonic show ovary and uterus Volume, endometrium thickness change.Blood FSH, E₂Being checked by the court's epi chamber, use luciferase linked immunosorbent assay, reagent is by method Biomerieux SA of state produces, and color ultrasound is Japan Nani17.Statistical counting data χ²Inspection, measurement data t is checked.

4 criterions of therapeutical effect

Effective: clinical symptoms and sign disappear, and Endocrinological inspection serum hormone is normal, menstrual onset and continuous more than 2 times Menorrhea, ovulation function recovers or gestation.Take a turn for the better: cardinal symptom and sign substantially alleviate, and menstruation recovery is risen, but the cycle is not Normally, Endocrinological inspection serum hormone takes a turn for the better or close to normal.Invalid: symptom, sign and Endocrinological inspection are not improved.

5 therapeutic outcomes

Two groups of comparitive study: two groups of comparitive study there was no significant difference (P ＞ 0.05).It is shown in Table 1.

1 liang of table group comparitive study example (%)

Group	n	Effective	Effectively	Invalid	Total effective rate
						Chinese drug-treated group	40	26	3	11	72.5%
Western medicine group	40	22	5	13	67.5%

Two groups of Serum Sex Hormones changes: serum E₂Significantly raised, FSH substantially reduces, and comparing before and after two groups of treatments has significantly Sex differernce (P ＜ 0.05)；

Compare after two groups of treatments and there was no significant difference.

2 liang of table group Serum Sex Hormones Indexes Comparison (-x ± s)

After two groups of medications, untoward reaction is compared: the untoward reaction of Chinese drug-treated group is less than Western medicine group, and two groups are compared and have significance poor Different, (P ＜ 0.05), it is shown in Table 3.

Untoward reaction comparative example after the group medication of 3 liang of table

Group	Feel sick	Headache	Mammary swelling pain	Edema	Abdominal distention	Leucorrhea is many
							Chinese drug-treated group	2	1	0	0	1	2
Western medicine group	7	6	2	3	2	4

Two groups of symptoms improvement are compared: two groups of transference cure rates compare significant difference (P ＜ 0.05), is shown in Table 4.

4 liang of table group symptom improves comparative example (%)

C discusses

Premature ovarian failure accounts for the 1%～3.8% of women's total number of persons, is the commonly encountered diseases in gynecological endocrine field.Owing to this type of is suffered from Person shows as early sending out or primary amenorrhea, estrogen are lowly caused perimenopausal symptom and infertile, therefore belong to traditional Chinese medical science amenorrhea, early send out absolutely Warp, menopausal syndrome, the category such as infertile.Owing to suffering from a deficiency of the kidney, function senilism is appointed in punching, and the dyspoiesis of menses causes uterus vessels of the uterus (blood Sea) hollow, depletion of blood can descend and menopause praecox."Fu Qingzhu works on obstetric" cloud: " the most disconnected through water, it appears that kidney water declines and dries up ".Because women is with blood It is main, and " YIN often being in shortage, YANG often being in excess ", therefore treatment should focus on tonifying kidney and nourishing yin, promoting blood flow to regulate menstruation.In tonifying kidney and nourishing yin Fang Fang, Radix Rehmanniae Preparata is grown Kidney yin, benefit marrow is monarch drug.Fructus Corni, Radix Polygoni Multiflori, Semen Cuscutae liver and kidney tonifying, support essence and blood.Radix Arnebiae (Radix Lithospermi), Pollen Tyjphae activating blood circulation to dissipate blood stasis, OK Blood nourishing the liver blood, adjusts punching to appoint.Herba Epimedii liver and kidney tonifying.Fructus Ligustri Lucidi, Herba Ecliptae are ERZHI WAN, effect tonifying kidney and nourishing liver benefit essence.Radix Stellariae Nourishing YIN and clearing away heat pathogenic fire purging.Radix Puerariae clearing away heat and promoting production of body fluid, has estrogen-like effects according to modern pharmacological research Radix Puerariae, in women's reproduction point Secrete and there is regulation effect.All medicines share to be played tonifying kidney and nourishing yin altogether and invigorates blood circulation, and takes good care of punching and appoints effect.Invigorating kidney for regulating menstruation Chinese medicine has and delays gonad Hypofunction effect, has again promotion ovarian function recovery effect, and kidney-nourishing tcm drug has certain tune to female gonad s function simultaneously By animal experiment study, joint effect, finds that kidney-reinforcing drug has promotion ovary LH/HCG receptor to increase phenomenon.Chinese medicine there may also be rush Entering the effect that fsh receptor produces, nourishing the liver and kidney, benefit intensive culture blood Chinese medicine have the effect promoting follicular development.With kidney-reinforcing drug in castration There is estrogen-like to react with mice, therefore think Chinese medicine scalable mankind's follicular development and ovulation.

Premature ovarian failure has obvious heterogeneity to clinical manifestation from the cause of disease to the course of disease, will carry out correct etiological diagnosis, Dialectical combine with differential diagnosis of diseases, take corresponding treatment strategies, to improve healthy or to delay gonad old and feeble.Modern study is thought, mends Kidney tune rushes Chinese medicine health invigorating, and regulation gonad axis and immunologic function, even delay gonad aging effect.Clinic shows, the kidney invigorating Safely, effectively, certainly, untoward reaction is few for treatment premature ovarian failure curative effect, in protection, recovers ovarian function side for the clinical practice of yin nourishing side Face has certain effect, and its curative effect is equal to simple western medicine treatment, and toxic and side effects is less, low price.

Claims (10)

1. the preparation method of the Chinese medicine composition treating women's ovary senilism, it is characterised in that described Chinese medicine composition bag Include endo-medicine and external used medicine；

Described endo-medicine is capsule, and its preparation process includes:

A, take crude drug put in ethanol soak, heat extract 2 times, for component 1；

B, medicinal residues extracting in water 2 times, thickening filtration, for component 2；

C, by above two component merge, concentrate, be dried to powder load capsule.

The preparation method of the Chinese medicine composition for the treatment of women's ovary senilism the most according to claim 1, it is characterised in that described In step a, take in the ethanol of crude drug addition 5-10 times amount and soak 1-2 hour, heating extraction 2 times, each 1-2 hour, merge Extracting solution, removes supernatant after standing, then 100-120 mesh filters, then the ultrafiltration post that molecular weight is 5000-10000 through damming surpasses Filter, when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C, the extractum of 1.36, stand for standby use, become component 1.

The preparation method of the Chinese medicine composition for the treatment of women's ovary senilism the most according to claim 1, it is characterised in that described In step b, the medicinal residues of the crude drug that above-mentioned ethanol extraction is crossed add weight 10 times amount water, heating and refluxing extraction 2 times, and each 1～2 is little Time, 2 extracting solution are merged and stands, become component 2.

The preparation method of the Chinese medicine composition for the treatment of women's ovary senilism the most according to claim 1, it is characterised in that described In step c, by component 1 and the merging of component 2, it is then placed in concentrating under reduced pressure tank, decompression recycling ethanol, is concentrated into liquor strength For 0.6g crude drug/mL, when the relative density of sucking filtration to filtrate is 20 DEG C 1.06；Above-mentioned filtrate is the macroporous absorption of 10L through volume After resin column absorption, with deionized water or the distilled water eluting of 10 times of resin column volumes, then by 95% second of 5 times of resin column volumes Alcohol eluting, collects ethanol elution, removes solvent, obtains medicated powder and load capsule after drying.

5. the preparation method of the Chinese medicine composition treating women's ovary senilism, it is characterised in that described Chinese medicine composition bag Include endo-medicine and external used medicine；

Described external used medicine is suppository, and its preparation method includes:

A. take the Pseudobulbus Bletillae (Rhizoma Bletillae), Indigo Naturalis, the Borneolum Syntheticum way of distillation are vaporized into crystallization, pulverize dusting cover stand-by, as component 1；

B. remaining raw material ethanol is extracted, concentrate, as component 2；

C. adding substrate after component 1, component 2 being mixed to be in harmonious proportion, suppository, sterilization pack are made in sizing.

The preparation method of the Chinese medicine composition for the treatment of women's ovary senilism the most according to claim 5, it is characterised in that described In step a, first the crude drug Pseudobulbus Bletillae (Rhizoma Bletillae), Indigo Naturalis, Borneolum Syntheticum steam method are distilled, obtain crystal, then pulverize, cross 100-120 Mesh sieve, standby as component 1；In described step b, then remaining crude drug is shredded, is soaked and be placed in lid stainless steel cask, Adding 70% ethanol, quality is coarse grained 0.8-1 times, stirs, moistening airtight placement more than 1 hour, makes fully to expand； By bottom percolator filter plate gauze bag parcel pave after again the drug sample after moistening expansion is mixed pine do dissipate, then with stainless Powder contained by steel spoon, loads percolator uniformly, fills 10-12 cm thick, even by T-bar pressure, then presses aforesaid operations, dress in layer Entering, suitably pressurize, medicated powder loads 2/3 eminence that must not exceed percolator；Medicated powder face upper cover rustless steel orifice plate fastens, and opens percolation Cylinder baiting valve below, and put a container, then it is slowly added into 70% ethanol；Air between medicated powder powder to be discharged, and There is ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open baiting valve and carry out percolation, control Percolation speed is generally 1000g medical material outflow per minute 2～3ml, and filtrate puts into liquid container, and is fallen by ethanol during emptying Enter liquid container；Percolate is merged and stands, as component 2；Component 1 component 2 is mixed into ointment；Choose substrate 50 DEG C～60 DEG C to add Heat is melted, and adds ointment cooling and shaping obtained above and makes class warhead shape suppository, and ultraviolet is killed virus.

The preparation method of the Chinese medicine composition for the treatment of women's ovary senilism the most according to claim 5, it is characterised in that described Substrate used by suppository is selected from: one or more in semi-synthetic fatty acid glyceride, poloxamer, Polyethylene Glycol, lanoline； Described substrate is heating and melting under the conditions of temperature 50 C～60 DEG C, adds the medicine mastic that described preparation method prepares, and water-bath is stirred Mixing uniformly, be mixed into the liquid of thickness, be poured in plastic shape-forming pipe, insert tampon, cooling and shaping makes bullet-shaped suppository； Described tampon is the afterbody tampon with cotton thread.

8. the Chinese medicine composition treating women's ovary senilism, it is characterised in that be made up of endo-medicine and external used medicine,

Described endo-medicine includes the crude drug of following weight portion: Radix Paeoniae Rubra 10～20 parts, Caulis Spatholobi 10～20 parts, Fructus Corni 10～ 20 parts, Herba Leonuri 10～20 parts, Radix Bupleuri 10～20 parts, Radix Arnebiae (Radix Lithospermi) 10～20 parts, Radix Rehmanniae Preparata 10～20 parts, Fructus Ligustri Lucidi 10～20 parts, drought Lotus grass 10～20 parts, Fructus Schisandrae Chinensis 10～20 parts, Fructus Rubi 10～20 parts, Semen Cuscutae 10～20 parts, Herba Epimedii 10～20 parts, Cortex Moutan 10～20 parts, Radix Puerariae 10～20 parts；

Described external used medicine includes the crude drug of following parts by weight: Radix Arnebiae (Radix Lithospermi) 10～20 parts, Radix Sophorae Flavescentis 10～20 parts, Fructus Cnidii 10～ 20 parts, Rhizoma Smilacis Glabrae 10～20 parts, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～20 parts, Folium Artemisiae Argyi 10～20 parts, Indigo Naturalis 10～20 parts, Spica Prunellae 10～20 parts, ice Sheet 10～20 parts, Herba Euphrasiae Regelii 10～20 parts.

Treat the Chinese medicine composition of women's ovary senilism the most according to claim 8, it is characterised in that described endo-medicine is also Crude drug including following weight portion: Radix Paeoniae Rubra 10～15 parts, Caulis Spatholobi 10～15 parts, Fructus Corni 10～15 parts, Herba Leonuri 10～ 15 parts, Radix Bupleuri 10～15 parts, Radix Arnebiae (Radix Lithospermi) 10～15 parts, Radix Rehmanniae Preparata 10～15 parts, Fructus Ligustri Lucidi 10～15 parts, Herba Ecliptae 10～15 parts, five Taste 10～20 parts, Fructus Rubi 10～20 parts, Semen Cuscutae 10～20 parts, Herba Epimedii 10～20 parts, Cortex Moutan 10～20 parts, Radix Puerariae 10 ～20 parts；

Described external used medicine also includes the crude drug of following parts by weight: Radix Arnebiae (Radix Lithospermi) 10～15 parts, Radix Sophorae Flavescentis 10～15 parts, Fructus Cnidii 10 ～15 parts, Rhizoma Smilacis Glabrae 10～15 parts, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～15 parts, Folium Artemisiae Argyi 10～20 parts, Indigo Naturalis 10～20 parts, Spica Prunellae 10～20 parts, Borneolum Syntheticum 10～20 parts, Herba Euphrasiae Regelii 10～20 parts.

Treat the Chinese medicine composition of women's ovary senilism the most according to claim 8, it is characterised in that described endo-medicine Also the crude drug of following weight portion is included: Radix Paeoniae Rubra 10～20 parts, Caulis Spatholobi 10～20 parts, Fructus Corni 10～20 parts, Herba Leonuri 10 ～20 parts, Radix Bupleuri 10～20 parts, Radix Arnebiae (Radix Lithospermi) 10～20 parts, Radix Rehmanniae Preparata 10～20 parts, Fructus Ligustri Lucidi 10～20 parts, Herba Ecliptae 10～20 parts, Fructus Schisandrae Chinensis 10～15 parts, Fructus Rubi 10～15 parts, Semen Cuscutae 10～15 parts, Herba Epimedii 10～15 parts, Cortex Moutan 10～15 parts, Radix Puerariae 10～15 parts；

Described external used medicine also includes the crude drug of following parts by weight: Radix Arnebiae (Radix Lithospermi) 10～20 parts, Radix Sophorae Flavescentis 10～20 parts, Fructus Cnidii 10 ～20 parts, Rhizoma Smilacis Glabrae 10～20 parts, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～20 parts, Folium Artemisiae Argyi 10～15 parts, Indigo Naturalis 10～15 parts, Spica Prunellae 10～15 parts, Borneolum Syntheticum 10～15 parts, Herba Euphrasiae Regelii 10～15 parts.