CN105920359A

CN105920359A - Traditional Chinese medicine suppository for treating premature ovarian failure of women and preparation method thereof

Info

Publication number: CN105920359A
Application number: CN201610539252.0A
Authority: CN
Inventors: 马佳
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-07-10
Filing date: 2016-07-10
Publication date: 2016-09-07

Abstract

The invention provides a traditional Chinese medicine suppository for treating premature ovarian failure of women. The suppository is prepared from the following raw materials in parts by weight: 10-20 parts of radix paeoniae alba, 10-20 parts of caulis spatholobi, 10-20 parts of herba leonuri, 10-20 parts of rhizoma cyperi, 10-20 parts of radices lithospermi, 10-20 parts of herba patriniae, 10-20 parts of glossy privet fruits, 10-20 parts of eclipta alba, 10-20 parts of mulberry, 10-20 parts of semen cuscutae, 10-20 parts of herba epimedii, 10-20 parts of cortex moutan, 10-20 parts of radix puerariae, 10-20 parts of radix sophorae flavescentis, 10-20 parts of fructus cnidii, 10-20 parts of rhizoma smilacis glabrae, 10-20 parts of bletilla striata, 10-20 parts of radix angelicae, 10-20 parts of fructus kochiae, 10-20 parts of dandelion, 10-20 parts of peppermint, 10-20 parts of eyebright and 10-20 parts of philippine flemingia roots. The medicinal materials are combined to achieve the effects of tonifying kidneys, nourishing yin, promoting blood circulation and regulating Chong-Ren. The traditional Chinese medicinal material for tonifying kidneys and regulating menstruation has the effects of delaying hypogonadotropic hypogonadism and promoting restoration of ovarian functions.

Description

A kind of Chinese medicine suppository treating women's ovary senilism and preparation method thereof

Technical field

The present invention relates to, containing the medicinal preparation deriving from plant composition, treat particularly to one Chinese medicine suppository of women's ovary senilism and preparation method thereof.

Background technology

Premature ovarian failure (POF) is the phenomenon of amenorrhea before referring to 40 years old that ovarian function failure is caused. Feature is primary or secondary amenorrhea raises with blood Gonadotropin Level and estrogen level reduces, and accompanies There are a series of low estrogen symptoms in various degree such as: hectic fever hyperhidrosis, flush, hyposexuality etc.. The average natural menopause age of women is 50～52 years old, and age of meuopause also exists race and Area distribution Difference, but its absolute value is more or less the same.Coulam etc. sum up the natural amenorrhea situation of 1858 example women, POF incidence rate less than 40 years old is 1%, and the POF incidence rate less than 30 years old is 1 ‰.Primary amenorrhea Middle POF accounts for 10%～28%, and in secondary amenorrhea, POF accounts for 4%～18%.Xu Ling etc. find Beijing area Women's POF incidence rate is 1.8%.As can be seen here, POF is the most much.

Premature ovarian failure (POF) is with change in menstrual cycle characteristics, estrogen deficiency, the rising of FSH level, estrogen Level drops to clinical characters.Western medicine diagnose standard is to occur before 40 years old closing for more than at least 4 months Warp, and have 2 times or above serum FSH ＞ 40U/L (2 inspection intervals more than 1 month), female two Alcohol horizontal ＜ 73.2pmol/L.Tcm diagnosis standard is cracked ends before 40 years old, infrequent menstruation, the most extremely Amenorrhea or suddenly menolipsis, warm sweating, and hectic fever is flushing, dysphoria with feverish sensation in the chest palms and soles, dizziness and tinnitus, soreness of the waist and knees Or metapedes is with pain, red dry stool of urinating, emaciated physique, vaginal dryness.B ultrasonic shows that uterus is less than normal or both sides ovum Nest is less than normal, does not finds follicle or monitors the most developable little follicle of rarely seen only a few, serum E continuously 2 low SI, FSH is significantly raised, red tongue or have crackle, tongue few, thready and rapid pulse or band string.

Premature ovarian failure accounts for the 1%～3.8% of women's total number of persons, is the commonly encountered diseases in gynecological endocrine field. Show as early sending out due to this type of patient or perimenopausal symptom that primary amenorrhea, estrogen are lowly caused and Infertile, therefore belong to traditional Chinese medical science amenorrhea, menopause praecox, menopausal syndrome, the category such as infertile.Owing to suffering from a deficiency of the kidney Function senilism is appointed in punching, and it is hollow that the dyspoiesis of menses causes uterus vessels of the uterus (sea of blood), depletion of blood can descend and Menopause praecox."Fu Qingzhu works on obstetric" cloud: " the most disconnected through water, it appears that kidney water declines and dries up ".Cause Women is based on blood, and " YIN often being in shortage, YANG often being in excess ", therefore treatment should focus on tonifying kidney and nourishing yin, Promoting blood flow to regulate menstruation.Radix Rehmanniae Preparata nourishing kidney-yin in tonifying kidney and nourishing yin Fang Fang, benefit marrow is monarch drug.Premature ovarian failure from the cause of disease to The course of disease has obvious heterogeneity to clinical manifestation, will carry out correct etiological diagnosis, dialectical and differential diagnosis of diseases Combine, take corresponding treatment strategies, to improve healthy or to delay gonad old and feeble.

Modern study is thought, Bushen Tiaochong Chinese medicine has health invigorating, regulates gonad axis and immunologic function, Even delay gonad aging effect.Clinic shows, the clinical practice of tonifying kidney and nourishing yin side safely, effectively, is controlled Treating premature ovarian failure curative effect certainly, untoward reaction is few, has certain in terms of protection, recovery ovarian function Effect, its curative effect is equal to simple western medicine treatment, and toxic and side effects is less, low price.

The most best therapeutic modality is the method using traditional Chinese medical science conditioning and treating.Chinese traditional treatment can be effective Solve the surface symptoms of disease, the basic factor caused a disease can be solved again thoroughly.Patient can also root According to the selection physiotherapy that concrete condition is suitable.But, patient it is noted that must not at will medication, Carry out under the guidance of expert.For physiotherapy, the most limited, it is impossible to excessively, the most unfavorable In the state of an illness, damage the health of patient.

Summary of the invention

The technical problem to be solved is, estrogen and progestogen replacement therapy is suffered from for young POF It is very important for person, i.e. can alleviate low estrogen symptom and Urogenital atrophy (for giving ovum Embryo transfer is prepared), can prevent again long term complication (osteoporosis, senile dementia etc.), The risk reduction 37% of colon cancer.But long-term HRT also has certain risk, such as carcinoma of endometrium and mammary gland The generation of cancer.The traditional Chinese medical science is particular about and is suited the remedy to the case, and individual variation is obvious, will carry out under the guidance of expert.Right In physiotherapy, the most limited, it is impossible to excessively, to be unfavorable for the state of an illness the most on the contrary, damage the health of patient. The present invention combines clinical experience for many years on the basis of traditional medicine, uses modern processing method meticulously to develop Form.The present invention each effective ingredient scientific compatibility to raw material medicine, focus of going directly, and instant effect, Side effect is little, can efficient solution except the misery of patient, reach the purpose of dispelling disease.

For solving above-mentioned technical barrier, the present invention provides a kind of Chinese medicine suppository treating women's ovary senilism, Its described Chinese medicine suppository can include the crude drug of following weight portion: the Radix Paeoniae Alba 10～20 parts, Caulis Spatholobi 10～ 20 parts, Herba Leonuri 10～20 parts, Rhizoma Cyperi 10～20 parts, Radix Arnebiae (Radix Lithospermi) 10～20 parts, Herba Patriniae 10～20 parts, Fructus Ligustri Lucidi 10～20 parts, Herba Ecliptae 10～20 parts, Fructus Mori 10～20 parts, Semen Cuscutae 10～20 parts, excessive sheep The leaves of pulse plants 10～20 parts, Cortex Moutan 10～20 parts, Radix Puerariae 10～20 parts, Radix Sophorae Flavescentis 10～20 parts, Fructus Cnidii 10～20 Part, Rhizoma Smilacis Glabrae 10～20 parts, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～20 parts, the Radix Angelicae Dahuricae 10～20 parts, the Fructus Kochiae 10～20 parts, Pu Herba Taraxaci 10～20 parts, Herba Menthae 10～20 parts, Herba Euphrasiae Regelii 10～20 parts, Radix Flemingiae Philippinensis 10～20 parts.

The Chinese medicine suppository of described treatment women's ovary senilism, its described Chinese medicine suppository can also include following heavy Amount part crude drug: the Radix Paeoniae Alba 10～15 parts, Caulis Spatholobi 10～15 parts, Herba Leonuri 10～15 parts, Rhizoma Cyperi 10～ 15 parts, Radix Arnebiae (Radix Lithospermi) 10～15 parts, Herba Patriniae 10～15 parts, Fructus Ligustri Lucidi 10～15 parts, Herba Ecliptae 10～15 parts, Fructus Mori 10～15 parts, Semen Cuscutae 10～15 parts, Herba Epimedii 10～15 parts, Cortex Moutan 10～15 parts, Radix Puerariae 10～ 20 parts, Radix Sophorae Flavescentis 10～20 parts, Fructus Cnidii 10～20 parts, Rhizoma Smilacis Glabrae 10～20 parts, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～20 parts, The Radix Angelicae Dahuricae 10～20 parts, the Fructus Kochiae 10～20 parts, Herba Taraxaci 10～20 parts, Herba Menthae 10～20 parts, Herba Euphrasiae Regelii 10～20 parts, Radix Flemingiae Philippinensis 10～20 parts.

The Chinese medicine suppository of described treatment women's ovary senilism, its described Chinese medicine suppository can also include following heavy Amount part crude drug: the Radix Paeoniae Alba 10～20 parts, Caulis Spatholobi 10～20 parts, Herba Leonuri 10～20 parts, Rhizoma Cyperi 10～ 20 parts, Radix Arnebiae (Radix Lithospermi) 10～20 parts, Herba Patriniae 10～20 parts, Fructus Ligustri Lucidi 10～20 parts, Herba Ecliptae 10～20 parts, Fructus Mori 10～20 parts, Semen Cuscutae 10～20 parts, Herba Epimedii 10～20 parts, Cortex Moutan 10～20 parts, Radix Puerariae 10～ 15 parts, Radix Sophorae Flavescentis 10～15 parts, Fructus Cnidii 10～15 parts, Rhizoma Smilacis Glabrae 10～15 parts, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～15 parts, The Radix Angelicae Dahuricae 10～15 parts, the Fructus Kochiae 10～15 parts, Herba Taraxaci 10～15 parts, Herba Menthae 10～15 parts, Herba Euphrasiae Regelii 10～15 parts, Radix Flemingiae Philippinensis 10～15 parts.

For solving above-mentioned technical problem, the present invention also provides for a kind of Chinese medicine suppository treating women's ovary senilism Preparation method, the preparation process of its described suppository includes:

A. take the Pseudobulbus Bletillae (Rhizoma Bletillae), the Radix Angelicae Dahuricae, the Herba Menthae way of distillation are vaporized into crystallization, pulverize dusting cover stand-by, as group Divide 1；

B. remaining raw material ethanol is extracted, concentrate, as component 2；

C. adding substrate after component 1, component 2 being mixed to be in harmonious proportion, suppository, sterilization pack are made in sizing.

In described step a, can first the crude drug Pseudobulbus Bletillae (Rhizoma Bletillae), the Radix Angelicae Dahuricae, Herba Menthae steam method be distilled, To crystal, then pulverize, cross 100-120 mesh sieve, standby as component 1.

In described step b, then remaining crude drug is shredded, is soaked and be placed in lid stainless steel cask, Adding 70% ethanol, quality is coarse grained 0.8-1 times, stirs, moistening airtight placement more than 1 hour, Make fully to expand；Filter plate gauze bag bottom percolator is wrapped up after paving again by the medicine after moistening expansion Sample is mixed pine and is done to dissipate, and then contains powder with rustless steel spoon, loads percolator uniformly, fills 10-12 cm thick, Even by T-bar pressure, then press aforesaid operations, loading in layer, suitably pressurize, medicated powder must not load Exceed 2/3 eminence of percolator；Medicated powder face upper cover rustless steel orifice plate fastens, and opens the blowing below percolator Valve, and put a container, then it is slowly added into 70% ethanol；Air between medicated powder powder to be discharged, And have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open Material valve carries out percolation, controls percolation speed and is generally 1000g medical material outflow per minute 2～3ml, and filtrate is put Enter in liquid container, and pour ethanol during emptying into liquid container；Percolate is merged and stands, as group Divide 2.

In described step c, component 1 component 2 is mixed into ointment；Choose 50 DEG C～60 DEG C heating of substrate to melt Changing, add ointment cooling and shaping obtained above and make class warhead shape suppository, ultraviolet is killed virus.

The preparation method of described treatment women's ovary senilism Chinese medicine suppository, its substrate used by described suppository can To be selected from: the one or many in semi-synthetic fatty acid glyceride, poloxamer, Polyethylene Glycol, lanoline Kind；Described substrate is heating and melting under the conditions of temperature 50 C～60 DEG C, adds what described preparation method prepared Medicine mastic, stirring in water bath is uniform, is mixed into the liquid of thickness, is poured in plastic shape-forming pipe, inserts Tampon, cooling and shaping makes bullet-shaped suppository；Described tampon is the afterbody tampon with cotton thread.

For solving above-mentioned technical problem, the present invention reoffers a kind of Chinese medicine suppository treating women's ovary senilism Preparation method, the preparation process of described suppository also includes:

B. by remaining raw material ethanol extraction, concentrate, obtain medicated powder as component 2；

In described step b, crude drug can be put into heating and refluxing extraction 2 times in ethanol, each 1～2 is little Time, 2 extracting solution are merged and stands；Being merged by above two extracting solution, concentrating under reduced pressure relative density is The filtrate of 1.38 when 70 DEG C, reclaims ethanol, by gained filtrate adjustment proportion to 1.60, is passed through spray drying Machine is spray-dried, and the compressed air pressure crossing spray dryer shower nozzle is 4kg/m², hothouse steam Stream temperature is 100 DEG C, obtains raw material medicated powder, as component 2.

The present invention uses stype and tampon integrated technique, compensate for the deficiency of ordinary suppository, and ordinary suppository is not Can effectively be delivered to the depths of vagina.Due to the self purification of vagina, in the presence of having foreign body, can increase Liquid secretion, gets rid of external at short notice by medicine, has both reduced curative effect, extends again the course for the treatment of, increases Add expense and pollute again underwear.And the present invention overcomes this technical barrier, the suppository made can give full play to Curative effect of medication, has safety good, the advantage that feasibility is good, focus of going directly, and reaches the purpose that gets rid of illness.

Tonifying kidney and nourishing yin of the present invention, promoting blood flow to regulate menstruation.Radix Rehmanniae Preparata nourishing kidney-yin in tonifying kidney and nourishing yin side, benefit marrow is monarch Medicine.Fructus Corni, Semen Cuscutae liver and kidney tonifying, support essence and blood.Radix Arnebiae (Radix Lithospermi), Radix Paeoniae Alba activating blood circulation to dissipate blood stasis, promoting the circulation of blood nourishing the liver Blood, adjusts punching to appoint.Herba Epimedii liver and kidney tonifying.Fructus Ligustri Lucidi, Herba Ecliptae are ERZHI WAN, effect tonifying kidney and nourishing liver Benefit essence.Cortex Moutan nourishing YIN and clearing away heat pathogenic fire purging.Radix Puerariae clearing away heat and promoting production of body fluid, has estrogen according to modern pharmacological research Radix Puerariae Sample effect, has regulation effect for women's reproductive endocrine.All medicines share to be played tonifying kidney and nourishing yin altogether and invigorates blood circulation, Take good care of punching and appoint effect.Invigorating kidney for regulating menstruation Chinese medicine has delay adenasthenia effect, has again promotion ovary merit Energy restitution, kidney-nourishing tcm drug has certain regulation effect to female gonad s function simultaneously, by dynamic Thing experimental studies have found that kidney-reinforcing drug has promotion ovary LH/HCG receptor to increase phenomenon.Chinese medicine there may also be Promote that the effect that fsh receptor produces, nourishing the liver and kidney, benefit intensive culture blood Chinese medicine have the work promoting follicular development With.

Detailed description of the invention

1. amenorrhea is divided into primary amenorrhea and secondary amenorrhea, and secondary amenorrhea occurred before 40 years old.By to greatly The investigation of the POF patient of sample does not has the tendency of distinctive irregular menstruation before finding amenorrhea.Have People is unexpected amenorrhea after the menstruation of rule, has plenty of and stops amenorrhea after contraceptive or childbirth, and have then exists The disorder in menstrual cycle and menstrual period is shown as before amenorrhea.

The most infertile some patients finds premature ovarian failure because of infertile medical.Infertile is that chain reaction in patients with premature ovarian is gone to a doctor With worried main cause.There are fertility and infertility, so suggestion has premature ovarian failure family history person Pregnancy should be planned as early as possible.

3. low estrogen symptom primary amenorrhea person low estrogen symptom (hectic fever and/or dyspareunia etc.) is rare (22.2%), also mostly using estrogen replacement therapy relevant with the past if had, secondary amenorrhea person is low Estrogen symptom common (85.6%).This is to be withdrawn the theory caused by estrogen with low estrogen symptom Consistent.These low estrogen symptoms also include atrophic vaginitis and the atrophic urethra such as frequent micturition, dysurea Scorching.

4. the performance of the autoimmune disease occurred together such as Addison disease, thyroid disease, diabetes, Lupus erythematosus, rheumatoid arthritis, vitiligo and crohn etc..Additionally also has adrenal insufficiency Concealment symptom, such as the alleviating of recent body weight, loss of appetite, indefinite abdominal pain, weak, skin Skin pigmentation increases the weight of and addicted to salt.

Caulis Spatholobi is bitter, sweet, temperature.Return liver, kidney channel.Cure mainly: relaxing muscles and tendons to promote blood circulation；Nourishing blood for regulating menstruation.Main brothers Numb；Quadriplegia；Rheumatic arthralgia；Women has through uncomfortable；Dysmenorrhea；Amenorrhea.Function: enrich blood, lives Blood, dredging collateral.For menoxenia, blood deficiency and yellow complexion, paralysis and numbness, rheumatic arthralgia." decoction pieces is newly joined ": Remove blood stasis, tissue regeneration promoting blood, fluent passages through which vital energy circulates.Control summer filthy infection, wind arthralgia due to stagnation of blood disease." modern Practical Chinese medicinal ": for by force The hematonic of strong property, it is adaptable to anemicus neural paralysis disease, such as limbs and soreness of waist and knee joint, apathy Deng.Again for menoxenia, menstruation apolipsis etc., there is the effect of invigorating blood circulation analgesia.

Fructus Corni, cures mainly pathogen cold and heat under the heart, warming middle-JIAO, by cold temperature numbness, goes three worms, and clothes have been made light of one's life by commiting suicide strong for a long time Cloudy benefit essence, settling five organs, the merit of logical nine orifices, only dribbling urination；Take improving eyesight, strength for a long time all the year round.Fructus Corni, Have another name called Fructus Corni, medicine Fructus Jujubae, real Fructus Jujubae, Fructus Corni, meat Fructus Jujubae etc., commonly use rare Chinese medicine for China, application With a long history.It is gentle with its benefit power, tonifying YANG and do not help fire, YIN nourishing and oiliness diaphragm, convergence and do not stay heresy Use is liked by successive dynasties medical science Deng special efficacy.Zhang Zhongjing has formulated " JINGUI SHENQI WAN " with Fructus Corni for monarch. According to chemical analysis, Fructus Corni contains the stronger cornin of physiologically active, tartaric acid, gallic acid, Herba Marsileae Quadrifoliae The effective ingredient such as fruit acid, resin, tannin and multivitamin, have enhancing immunity, antiinflammatory, antibacterial etc. Pharmacological action, is Herba indigoferae Pseudotinctoriae conventional in tcm clinical practice.

Fructus Ligustri Lucidi, sweet-bitter flavor, cool in nature, return Liver and kidney warp, there is nourishing the liver and kidney, merit that tonifying YIN nourishes blood.Existing Proving for medical research, Fructus Ligustri Lucidi includes ursolic acid, mannitol, glucose, fatty oil etc., have heart tonifying, Diuresis and hepatoprotective effect, can make the leukopenia quantity caused because of chemotherapy raise, and to golden yellow Fructus Vitis viniferae Coccus, escherichia coli have inhibitory action.Containing the acid of oleanolic acid Fructus Ligustri Lucidi, nuezhenoside, Bears in Fructus Ligustri Lucidi Fruit acid, stearic acid, oleic acid, mannitol, betulin, phospholipid and saccharide such as Fructus Ligustri Lucidi polysaccharide, Ah Draw uncle sugar, glucose etc., possibly together with 15 kinds of aminoacid, 11 kinds of trace element and volatile oil etc..Research Think that Fructus Ligustri Lucidi has blood sugar lowering and blood fat reducing and has study of anti-atherogenic effect, the most antibacterial, antiviral Effect, Fructus Ligustri Lucidi decoct is to staphylococcus aureus, shigella flexneri, Bacillus typhi, bacillus pyocyaneus Inhibitory action is all had with escherichia coli.Isocratic inhibitory action during Type B adenovirus is had.Leukocyte increasing: Fructus Ligustri Lucidi and component preparation thereof all have certain impact to hemocyte, and leukopenia caused by chemotherapy is had by it Rising effect.Compound recipe containing Fructus Ligustri Lucidi also has effect in increasing leukocyte.Anti thrombotic action: oleanolic acid can add The mobility of fast platelet cell, reduces the collision between platelet, is allowed to be difficult to adhesion and gathering, It is less susceptible to deposit in tunica intima, slows down or prevent thrombosis, the deposition of lipid inner membrance can be reduced again, Thrombotic disease for preventing and treating old people provides part Experiment foundation.Defying age raising immunity: Fructus Ligustri Lucidi Sub-decoct can make mouse immune organ thymus, spleen weight increase；Significantly improve mice serum hemolysin to resist Body activity, the impact that mouse spleen lymphocyte produces interleukin-2 has dual regulation.Fructus Ligustri Lucidi There is significant immunological enhancement, remove free radical thus defying age.Application Fructus Ligustri Lucidi preventing and treating: painstaking effort Pipe disease, coronary heart disease, hyperlipemia, diabetes, thrombocytopenia, leukopenia, chronic Nephritis, chronic bronchitis, intractable insomnia, Chronic Benzene Poisoning, tumor, malnutrition, immunity merit Can low disease, seborrheic dermatitis

Cortex Moutan, Chinese medicine name.It is dried root bark for cohosh Paeonia suffruticosa.Originate in the ground such as Anhui, Shandong.Autumn Excavate root season, remove radicula, strip root bark, dry.Life is used or is fried and uses.Compendium of Material Medica: " grow Cloudy pathogenic fire reducing, solves speckle poison, throat, diuresis stasis.Descendant is specially to control fire mutually with yellow tiller, does not knows Cortex Moutan Merit more win also.Red colored person profit, the person of spending in vain mends, and people the most seldom realizes, and preferably distinguishes it." modern study, institute Antiinflammatory action is all had containing paeonol and glucosides constituents in addition thereof；The methanolic extract of Cortex Moutan has suppression Platelet；Paeonol has central inhibitory actions such as calmness, cooling, antipyretic, analgesia, spasmolytic and anti- Atherosclerosis, diuresis, antiulcer etc. act on.

Radix Salviae Miltiorrhizae is bitter, is slightly cold.GUIXIN, Liver Channel.Stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, clear away heart-fire relieving restlessness.For the moon Through uncomfortable, amenorrhea dysmenorrhea, lump in the abdomen, chest and abdomen twinge, pyretic arthralgia pain, skin infection swells and ache, vexed not Sleep.For costa sternales hypochondriac pain, rheumatic arthralgia, lump in the abdomen lumps, and skin infection swells and ache, and tumbling down is grieved, menoxenia, Amenorrhea dysmenorrhea, puerperal the stasis of blood pain etc..Treatment costa sternales pain, lump in the abdomen caking, and menoxenia, through amenorrhea Pain has good effect." detailed outline " carries: Radix Salviae Miltiorrhizae, and by " the sensible opinion of married woman " cloud, SIWU TANG controls gynecopathy, Paying no attention to antenatal puerperal, how many through water, the most generally applicable, only taste Radix Salviae Miltiorrhizae dissipates, and cures mainly same.Gai Dan Ginseng can break stagnated blood, mends fresh blood, and peaceful tire, fall stillborn fetus, only metrorrhagia dysentery, regulating menstruation arteries and veins.

Fructus Rubi plant can be used as medicine, and has multi-medicament to be worth, and its fruit has the effect of kidney invigorating and YANG supporting.Cover basin Seed oil belongs to unsaturated fatty acid, can promote that the severe that of prostatic secretions covers.Effect: the liver and the kidney tonifying, controlling nocturnal emission with astringent drugs Reducing urination, improving eyesight.Cutting into slices with rat, the vaginal smear of rabbit and inner membrance and make treatment index, Fructus Rubi seemingly has female Hormone-like effect.Bacteriostasis 100% decoct flat board burrows method, and staphylococcus is had inhibitory action, Vibrio cholera is also had inhibitory action.Estrogen-like effects rat, the vaginal smear of rabbit and inner membrance section Showing etc. index, Fructus Rubi seemingly has estrogen-like effects.

Herba Leonuri acrid in the mouth is bitter, cool.Invigorate blood circulation, silt of dispelling, regulating menstruation, disappear water.Treatment menoxenia, tire Leakage difficult labour, retention of placenta, puerperal blood faints, abdominal pain due to blood stasis, metrorrhagia and metrostaxis, hematuria, rushes down blood, carbuncle skin ulcer Infections.Herba Leonuri alkalescence subcutaneous injection has central inhibitory action, and MLD is 0.4-0.6g/kg.Intravenous rabbit is injected Leonurine 1mg/kg, it is seen that urine volume dramatically increases.Leonurine can make Sanguis Leporis seu oryctolagi suspension when higher concentration There is haemolysis.Leonurine infusion tube dilution method 1: 13-1: 10, to achorion schoenleinii, Pilus Caprae seu Ovis sample sporidiole bacteria, epidermophyton rubrum, starlike nocadia all have inhibitory action.Herba Leonuri decoct Use flat board paper disk method, escherichia coli, dysentery bacterium are had inhibitory action.Herba Leonuri can go life of becoming silted up Newly, promoting blood flow to regulate menstruation, containing the various trace elements such as selenium, manganese, can antioxidation, anti-aging, have quite Good beneficial face is improved looks, anti-aging effect.

Radix Puerariae, Chinese medicine name.For the dry root of legume pueraria lobata, practise and claim Herba Gelsemii Elegantis.Autumn, season in winter two excavate, Take advantage of fresh-cut and become sheet or fritter；It is dried.Sweet, pungent, cool.There is an expelling pathogenic factors from muscles for reducing heat, rash, promoting the production of body fluid to quench thirst, The merit of yang invigorating antidiarrheal.Being usually used in exterior syndrome heating, stiff nape and back, measles without adequate eruption, calentura is thirsty, and the deficiency of YIN disappears Yearningly, diarrhea of heat type hematodiarrhoea, diarrhea due to hypofunction of the spleen.

Semen Cuscutae: Cuscuta chinensis be dicotyledon scammonia section plant Semen Cuscutae, Cuscuta australis, The seed of cuscuta japonica Choisy etc., can the kidney invigorating and essence nourishing, nourishing the liver to improve visual acuity, effect of solid tire stopping leak.Nourishing the liver and kidney, solid Final minification is urinated, antiabortive, improving eyesight, antidiarrheal.For impotence and seminal emission, dripping urination, enuresis frequent micturition, the acid of waist knee joint Soft, blurred vision tinnitus, vaginal bleeding due to deficiency of the kidney during pregnancy, frequent fetal movement, spleen kidney vaginal bleeding during pregnancy, frequent fetal movement, spleen are suffered from a deficiency of the kidney and are rushed down；Outward Curing vitiligo.Former medicine sieve is removed spare, picks decontamination, dry.

Herba Ecliptae grass is the conventional liver-nourishing kidney-boosting of the traditional Chinese medical science, cooling blood for hemostasis medicine.To the head caused by the hepatic and renal YIN deficiency Dusk dizzy, all diseases of odontoseisis, aching pain in waist and back, flaccidity and weakness of the lower limbs and heat in blood caused by multiple go out blood disorder have Good efficacy.Herba Ecliptae contains the chemistry such as volatile oil, tannin, Saponin, Herba Ecliptae element and vitamin A and becomes Point.Animal experiment has haemostatic effect, in vitro tests that staphylococcus aureus is had inhibitory action.

Herba Epimedii is Berberidaceae plant Herba Epimedii, Epimedium sagittatum, Epimedium wushanense, Herba Epimedii etc. The stem and leaf of multiple Herba Epimedii.Taste is returned through acrid, sweet, warm.Liver, kidney channel is returned to cure mainly function: kidney-replenishing, Bone and muscle strengthening, wind-damp dispelling.Herba Epimedii has slow down aging, blood pressure lowering, blood sugar lowering, blood fat reducing.Improve brain Hypoxic-ischemic, reduction blood viscosity, suppression ex vivo thrombosis, the stimulation synthesis of bone marrow DNA, increase Peripheral blood leucocyte and the quantity of marrow hemopoietic stem cells, the immunity that improves, promotion bone growth, prevention Osteoporosis, antiinflammatory, antiviral, antitumor, the side effect of antagonism cancer therapy drug.

Rhizoma Smilacis Glabrae is sweet light, flat.Gui Jing: Liver Channel；Stomach warp；Spleen channel.Function: removing toxic substances, dehumidifying, Li Guan Joint.Cure mainly: control syphilis, stranguria with turbid discharge, muscles and bones spasm and pain, tinea pedis, treat furuncle, carbuncle, scrofula, syphilis And the limbs contracture caused by mercurialism, bones and muscles pain." book on Chinese herbal medicine justice ": Rhizoma Smilacis Glabrae, dampness removing reduces phlegm and internal heat, Network can be entered, search to pick and damp and hot accumulate poison.It solves d hydrargyrum, Calomelas poison person, and it is up that receive poison with ascending, and This leads under profit as business to ooze, therefore specially controls Fructus Myricae rubrae skin infection, gos deep into hundred networks, and arthralgia is the most rotten, again Poison fire is up, and throat pain is burst, and all dislike disease.

Fructus Cnidii is pungent, bitter, temperature；Slightly poisonous.Return kidney channel.Cure mainly warming the kidney to invigorate YANG, dampness, dispel the wind, Parasite killing.For sexual impotence, cold womb, cold-damp leukorrhagia, arthralgia chiefly caused by damp pathogen lumbago；External treatment vulval eczema, married woman's pudendal pruritus； Trichomonal vaginitis, is gynecological external use good medicine." Bencao Jingshu " carries: Fructus Cnidii, and bitter in the mouth is put down；" not Record " pungent sweet nontoxic；Modern its abnormal smells from the patient in detail, when warm-dryness syndrome of must holding concurrently, sun is also.Therefore housewife people's the moon swells and ache, man Impotence pruritus due to damp pathogen, eliminating impediment gas, profit joint, malignant boil." not Lu " warming the middle warmer and descending qi, makes married woman's clothes heat, man Son is cloudy strong, makes us there is son.Lid can dehumidify with tongue, temperature energy cold expelling, the pungent kidney that can moisten, sweet energy strengthening the spleen, therefore energy Except the married woman man wet disease of viscera connecting with this channel of all deficiency and coldness.Cold-damp was both removed, then disease is gone, performance Yiyang, thus can disease, And have tonification also.

Radix Sophorae Flavescentis is bitter, cold.GUIXIN, liver, stomach, large intestine, bladder are through heat clearing and damp drying, parasite killing, diuresis. For hematodiarrhoea, have blood in stool, jaundice urine retention, leucorrhea with red and white discharge, swelling of the vulva pudendal pruritus, eczema, eczema, skin pruritus, Scabies leprosy；External treatment colpitis mycotica, is often used in combination with Cortex Phellodendri, is to treat colpitic external woman Section's good medicine.

Pseudobulbus Bletillae (Rhizoma Bletillae) property is bitter, sweet, puckery, is slightly cold.Return lung, liver, stomach warp.Function cures mainly astringing to arrest bleeding, detumescence Granulation promoting.Spit blood for hemoptysis, traumatic hemorrhage, sore swollen toxin, chapped skin；Pulmonary tuberculosis hemoptysis, ulcer Sick hemorrhage.Shennong's Herbal: " main carbuncle, malignant boil, lose cellulitis, the dead flesh of impairment of YIN, pathogen in stomach "； Compendium of Material Medica: " abnormal smells from the patient (root) is bitter, flat, nontoxic.

Radix Arnebiae (Radix Lithospermi) nature and flavor: bitter, cold, Gui Jing: enter percardium, Liver Channel.Function cures mainly: removing heat from blood, invigorates blood circulation, Heat clearing away, removing toxic substances.Controlling warm macule, jaundice due to damp-heat, purpura, tell, be defeated in battle, hematuria, stranguria with turbid discharge, accumulation of heat is just Secret, burn, eczema, erysipelas, carbuncle and ulcer." herbal classic ": " main trusted subordinate's pathogen, five types of jaundice, invigorating the spleen and replenishing QI, Profit nine orifices, waterway.″

Herba Euphrasiae Regelii, formal name used at school: Euphrasia pectinata Ten, for annual herb plant, herb tool medicine By value.Herba Euphrasiae Regelii extract is the aerial parts of the herb of Herba Euphrasiae Regelii, have eye pouch, disappear fatigue Effect.Bitter in the mouth；Cold nature, Gui Jing, return bladder warp, function cures mainly heat-clearing and toxic substances removing；Diuresis.Main calentura Thirsty；Headache；Cough due to lung-heat；Laryngopharynx swelling and pain；Pyretic stranguria；Dysuria；Aphtha；Carbuncle.

Herba Taraxaci, dandelion herb, rich in vitamin A, vitamin C and potassium, also contains ferrum, calcium, vitamin B2, vitamin B1, magnesium, vitamin B6, folic acid and copper.Concrete constituent content is mainly moisture, Every 60 grams of dandelion herb leaf moisture contents 86%, 1.6 grams of protein, carbohydrate 5.3 grams, heat there are about 108.8 kilojoule.Containing taraxol, taraxacin, choline, organic acid, inulin in Herba Taraxaci plant Etc. multiple health-nutrition composition.Nature and flavor are sweet, micro-hardship, cold.Return liver, stomach warp.There are diuresis, cathartic, move back The effect such as jaundice, function of gallbladder promoting.Control pyretic toxicity, carbuncle, skin infection, abscess of internal organ, conjunctival congestion and swelling pain, damp and hot, jaundice, The puckery pain of dribbling urination, furuncle swelling toxin, acute mastitis, scrofula, toothache, conjunctival congestion, pharyngalgia, lung abscess, acute appendicitis, Jaundice due to damp-heat, the puckery pain of pyretic stranguria.Control acute mastitis, lymphadenitis, scrofula, furuncle carbuncle toxin, acute knot Film is scorching, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urine Road feel dye etc..Herba Taraxaci is raw-eaten, stir-fry and eat, cook soup, is the plant of dietotherapeutic.

The Radix Angelicae Dahuricae, Latin literary fame: Angelica dahurica (Fisch.ex Hoffm.) Benth.et Hook.f. Ex Franch.et Sav, is used as medicine with root, the function such as the dehumidifying that gets rid of illness, evacuation of pus granulation promoting, promoting blood circulation and stopping pain.Main Control anemofrigid cold, headache, rhinitis, toothache.The diseases such as leucorrhea with red and white discharge, bitterly furuncle and phyma poison, also can make spice. " detailed outline ": the Radix Angelicae Dahuricae, the white acrid in the mouth of color, row hands sun is bright；Gas warm in nature is thick, and row foot sun is bright；Reach on fragrance, Enter lung meridian.Such as head, mesh, eyebrow, all diseases of tooth, the wind heat of three warps is also；As leakage, band, carbuncle all Disease, three through damp and hot also；Wind heat person is pungent to dissipate it, and damp and hot person's temperature is with except it.For positive bright principal agent, therefore again Can control disorders of blood, fetal diseases, and evacuation of pus granulation promoting pain relieving.Control nasal sinusitis, epistaxis, toothache, supraorbital bone pain, large intestine Constipation due to pathogenic wind, bleeding urine, married woman's blood dizziness due to wind pathogen fortune, regurgitation of food from the stomach tells food；Solution arsenic poison, snakebite, cutter arrow incised wound.

Herba Menthae is one of China's conventional Chinese medicine.It is pungent cool property sweating antipyretic medicine, controls influenza, head Pain, conjunctival congestion, fever of the body, throat, dental bed such as swell and ache the disease.External can training the mind dysmenorrhoea, skin pruritus, erythra With eczema etc..Usually with Herba Menthae for tea, clear away heart-fire improving eyesight.Herba Menthae is China's conventional Chinese medicine, and children's tender stem point can Making vegetable edible, herb can be used as medicine again, and cure cold heating laryngalgia, headache, conjunctival congestion with ophthalmalgia, myalgia, skin The diseases such as rubella is scratched where it itches, measles without adequate eruption, the most effective to carbuncle, cellulitis, scabies, tinea, dermatitis rhus in addition.Herba Menthae contains Menthol, this material can fresh breath there is the multiple property of medicine, stomachache, gallbladder problem such as spasm can be alleviated, Also have antisepsis and sterilization, diuresis, reduce phlegm, stomach invigorating and the effect such as aid digestion.A large amount of edible Herba Menthaes may result in Insomnia, but low dose of eating conduces to sleep.[

The Fructus Kochiae, Chinese medicine name.For being dried to of chenopod Fructus Kochiae Kochia scoparia (L.) Schrad. Ripe fruit.Gather during fruit maturation in autumn plant, dry, lay fruit, remove impurity.Acrid in the mouth, hardship, Cold in nature.Return kidney, bladder warp.Clearing away heat-damp and promoting diuresis, dispelling wind for relieving itching.Cure mainly for difficulty and pain in micturition, pudendal pruritus band Under, rubella, eczema, skin pruritus.Compendium of Material Medica: cystocele abdominal mass.The critical person of hernia, fries and grinds wine Clothes；Inguinal hernia the moon ovum disease, the same to Rhizoma Atractylodis Macrocephalae, Cortex Cinnamomi end clothes." herbal classic ": bladder heat, diuresis, invigorating middle warmer benefit Vital essence.Take normal hearing and eyesight for a long time, make light of one's life by commiting suicide resistance to always.

Herba Patriniae nature and flavor hardship is put down, and has heat-clearing and toxic substances removing, effect of evacuation of pus removing blood stasis with potent drugs.Can cure acute appendicitis, dysentery, Leucorrhea with red and white discharge, the stagnant stomachache of the stasis of blood in puerperal, conjunctival congestion and swelling pain.Enter stomach, large intestine, Liver Channel.Cure mainly heat-clearing and toxic substances removing, Eliminating carbuncle evacuation of pus, blood circulation promoting competent silt.For acute appendicitis, lung abscess and sore and toxic, the stagnant caused chest and abdomen pain of the excess-heat stasis of blood Bitterly, the disease such as stagnant stomachache of the stasis of blood in puerperal.

The Radix Paeoniae Alba enters liver, spleen channel, nourishing blood to suppress the hyperactive liver, and slow middle pain relieving, yin fluid astringing receives antiperspirant.Control the chest and abdomen pain over the hypochondriac region, Dysentery is suffered from abdominal pain, spontaneous sweating, fever due to yin deficiency, menoxenia, metrorrhagia, leukorrhagia, and married woman's blood closes obstructed, Repercussive blood, can lose pus, benefit woman's blood." Japan hanako materia medica ": control wind, mend labor, all diseases of main woman, And antenatal after all diseases, Tong Yueshui, bring down a fever, relieving restlessness, QI invigorating, the hot disease of sky row, pestilence miasma, mania and irritation, married woman Blood is transported, and hemorrhoidal hamorrhage, rushes down blood, hemorrhoid complicated by anal fistula.Carbuncle on the back, skin ulcer scabies, headache, improving eyesight, conjunctival congestion exerts meat.Redness of the skin or complexion person Many QI invigorating, white person controls blood.

The pungent micro-hardship of Rhizoma Cyperi is sweet, and flat function cures mainly: resolving depression of regulating the flow of vital energy, menstruction regulating and pain relieving.For stagnation of QI due to depression of the liver, breast, The side of body, abdominal distention, dyspepsia, menoxenia, amenorrhea dysmenorrhea, colic of cold type stomachache, distending pain of the breast." guiding principle Mesh " carry it: scattered seasonal epidemic pathogens is trembled with fear epidemic disease, and profit three is burnt, solves six kinds of stagnation-syndromes, and the diet that disappears gathers, phlegm retention feeling of fullness, edema of dorsum of the foot, Abdominal distention, tinea pedis, only trusted subordinate, limbs, head, mesh, tooth, all pains of ear, ulcer sores, spit blood, hematochezia, Hematuria, married woman's bleeding not during menses, wait uncomfortable by the moon, all kinds of diseases and ailments in puerperal before tire.

Fructus Mori, have and improve skin (including scalp) blood supply, nutrition skin, make skin delicate and black Send out the effect of grade, and can slow down aging.Fructus Mori are that middle-aged and elderly people is healthy and strong U.S. face, the good fruit of defying age and good medicine. Often food Fructus Mori can be with improving eyesight, the symptom that relieving eye strain is dry and astringent.Fructus Mori have immunologic enhancement.Mulberry Shen has effect of gain to spleen, and hemolytic reaction is had potentiation, can prevent human body artery hardening, bone Bone arthrosclerosis, enhances metabolism.It can promote the growth of erythrocyte, prevents leukopenia, And the diseases such as treatment diabetes, anemia, hypertension, hyperlipidemia, coronary heart disease, neurasthenia are had auxiliary Assist is imitated.Fructus Mori has the effects such as promoting the production of body fluid to quench thirst, facilitating digestion, help defecation, and proper amount of edible can promote Gastric secretion, stimulates enterokinesia and releases scorching.Motherland's medical science is thought, Fructus Mori are sweet in flavor and cold in property, has benefit The effects such as liver kidney tonifying, intestine moistening of promoting the production of body fluid, black hair improving eyesight.

Radix Flemingiae Philippinensis, formal name used at school: Flemingia philippinensis Merr.et Rolfe.Flos Rosae Multiflorae suborder bean Papillionoideae race of section Phaseoleae Moghania Radix Flemingiae Philippinensis.Root hyoscine, has expelling wind and removing dampness, relaxing muscles and tendons to live Network, bone and muscle strengthening, anti-inflammatory analgetic etc. act on.Miao Ethnomedicine: radical cure rheumatic ostalgia, lumbar muscle strain, hemiplegia, And chronic tracheitis, mastitis, poliomyelitis sequela, venom " Hunan indigo plant is examined ".BAIYAO: Radical cure rheumatic arthritis, lumbar muscle strain, metrorrhagia, leucorrhea, traumatic injury " record of Yunnan medicine ".Dong's medicine: Radical cure rheumatic ostalgia, pelvic bone pain, soft tired dizzy, myasthenia of limbs, dyspepsia " osmanthus medicine volume ".

The embodiment 1 of suppository of the present invention

Pseudobulbus Bletillae (Rhizoma Bletillae) 1000g, Radix Angelicae Dahuricae 1000g, the Herba Menthae 1000g way of distillation are vaporized into crystallization, then pulverize, Cross 100-120 mesh sieve, standby as component 1；Again by remaining crude drug Radix Paeoniae Alba 1000g, Caulis Spatholobi 1000g, Herba Leonuri 1000g, Rhizoma Cyperi 1000g, Radix Arnebiae (Radix Lithospermi) 1000g, Herba Patriniae 1000g, Fructus Ligustri Lucidi 1000g, Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) Grass 1000g, Fructus Mori 1000g, Semen Cuscutae 1000g, Herba Epimedii 1000g, Cortex Moutan 1000g, Radix Puerariae 1000g, Radix Sophorae Flavescentis 1000g, Fructus Cnidii 1000g, Rhizoma Smilacis Glabrae 1000g, Fructus Kochiae 1000g, Herba Taraxaci 1000g are little Rice grass 1000g, Radix Flemingiae Philippinensis 1000g chopping, is soaked and has been placed in lid stainless steel cask, added 70% ethanol Liquid, quality is coarse grained 0.8-1 times, stirs, moistening airtight placement more than 1 hour, makes fully Expand；Again the drug sample after moistening expansion is mixed after bottom percolator, filter plate gauze bag parcel is paved Pine does to dissipate, and then contains powder with rustless steel spoon, loads percolator uniformly, fills 10-12 cm thick, with T-shaped Rod pressure is even, then presses aforesaid operations, and loading in layer is suitably pressurizeed, and medicated powder loads must not exceed and oozes Filter 2/3 eminence of cylinder；Medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and Put a container, be then slowly added into 70% ethanol；Air between medicated powder powder to be discharged, and have second Alcohol flows out about about 20L, closes baiting valve, covers cylinder of filtering, dipping 24 hours, then opens baiting valve and enter Row percolation, controls percolation speed and is generally 1000g medical material outflow per minute 2～3ml, and filtrate puts into reservoir In cylinder, and pour ethanol during emptying into liquid container；Percolate is merged and stands, as component 2； Component 1 component 2 is mixed into ointment；Choose 50 DEG C～60 DEG C heating and melting of substrate, add medicine obtained above Cream cooling and shaping makes class warhead shape suppository, and ultraviolet is killed virus.Substrate used by described suppository is selected from: half One or more in synthctic fat acid glyceride, poloxamer, Polyethylene Glycol, lanoline；Described base Matter is heating and melting under the conditions of temperature 50 C～60 DEG C, adds the medicine mastic that described preparation method prepares, Stirring in water bath is uniform, is mixed into the liquid of thickness, is poured in plastic shape-forming pipe, inserts tampon, cooling Bullet-shaped suppository is made in sizing；Described tampon is the afterbody tampon with cotton thread.

The detailed directions of described suppository is as follows: drug suppository of the present invention is removed cabinet, takes out stype, Pull-out afterbody cotton thread, includes in stype bottom vagina, and the most once, cotton thread is stayed external, and morning next day rises Pull-out tampon, continuous use 7 days is a course for the treatment of.

The embodiment 2 of suppository of the present invention

Pseudobulbus Bletillae (Rhizoma Bletillae) 1100g, Radix Angelicae Dahuricae 1200g, the Herba Menthae 1200g way of distillation are vaporized into crystallization, then powder Broken, cross 100-120 mesh sieve, standby as component 1；Again by remaining crude drug Radix Paeoniae Alba 1200g, Sanguis Gallus domesticus Rattan 1200g, Herba Leonuri 1200g, Rhizoma Cyperi 1200g, Radix Arnebiae (Radix Lithospermi) 1200g, Herba Patriniae 1200g, Fructus Ligustri Lucidi 1200g, Herba Ecliptae 1200g, Fructus Mori 1200g, Semen Cuscutae 1200g, Herba Epimedii 1200g, Cortex Moutan 1200g, Radix Puerariae 1200g, Radix Sophorae Flavescentis 1200g, Fructus Cnidii 1200g, Rhizoma Smilacis Glabrae 1200g, Fructus Kochiae 1200g, Pu Herba Taraxaci 1200g, Herba Euphrasiae Regelii 1200g, Radix Flemingiae Philippinensis 1200g shred, and put into heating and refluxing extraction 2 in ethanol Secondary, each 1～2 hour, 2 extracting solution are merged and stands；Above two extracting solution is merged, subtracts Pressure concentrates the filtrate of 1.38 when relative density is 70 DEG C, reclaims ethanol, is arrived by gained filtrate adjustment proportion 1.60, it is passed through spray dryer and is spray-dried, the compressed air pressure crossing spray dryer shower nozzle is 4kg/m², hothouse hot stream temperature is 100 DEG C, obtains raw material medicated powder, as component 2.Component 1 group 2 are divided to be mixed into ointment；Choose 50 DEG C～60 DEG C heating and melting of substrate, add ointment obtained above cooling Class warhead shape suppository is made in sizing, and ultraviolet is killed virus.Substrate used by described suppository is selected from: semi-synthetic fat One or more in fatty acid glyceride, poloxamer, Polyethylene Glycol, lanoline；Described substrate is in temperature Heating and melting under the conditions of spending 50 DEG C～60 DEG C, adds the medicine mastic that described preparation method prepares, and water-bath is stirred Mix uniformly, be mixed into the liquid of thickness, be poured in plastic shape-forming pipe, insert tampon, cooling and shaping system Become bullet-shaped suppository；Described tampon is the afterbody tampon with cotton thread.

Toxicology test:

Test example 1: acute skin irritation test of the present invention is reported

1, experiment purpose: detect suppository of the present invention to the stimulation/corrosiveness of laboratory animal skin and intensity

2, material and method:

2.1. tested material: title: suppository of the present invention (embodiment 1 method prepares)

2.2. laboratory animal: one grade of big ear white race rabbit, 10, female, body weight 2.1-2.3kg；By green grass or young crops Island University Medical College biology department Experimental Animal Center provides；

2.3. test method:

Select the healthy rabbits 10 that skin is intact, in testing first 24 hours by its spinal column both sides, back quilt Hair is cut, and removes the left and right each about 4cm × 4cm of gross area.Take tested material 0.2ml during test and be applied to 2.5cm On two layers of gauze of × 2.5cm, being applied to skin of unhairing surface, side, oilpaper covers, immobilization with adhesive tape. Opposite side skin is as blank.After closing 4 hours, dismantle, and go residual with warm water cleaning Staying tested material, position dermoreaction is smeared in the treatments in 1,24 and 48 hours after removing tested material, and Scoring.

3, experimental result: any erythema does not occurs in laboratory animal skin, red and swollen, inflammation phenomenon.

4, brief summary: judge according to skin wound repair standards of grading in " disinfection technology standard " (1999), Ointment of the present invention belongs to nonirritant to animal skin stimulus intensity.

Embodiment 2: vaginal mucomembranous irritant test result

1. test method:

After laboratory animal is quarantined 3 days, select 18 above-mentioned healthy rabbits, be randomly divided into three groups, often group Each 6, it is respectively as follows: blank, excipient control, suppository group of the present invention (by application 5 times of concentration of liquid). It is placed in excipient control group with sterile saline and tested material respectively with appropriately sized sterilizing cotton sliver leaching With tested material treated animal intravaginal, contact with vaginal mucosa 6 hours, after 24 hours, put to death animal.Take out Topical vaginal tissue, treats pathological changes situation.

2, result of the test: vaginal mucomembranous irritant test result

Embodiment 3: skin anaphylactic test result

Use suppository of the present invention, carry out inducing and exciting contact test, treat behind 24 hours and 48 hours Test mice, all loseing skin has the phenomenon such as erythema and edema, with negative control group zero difference.Result table Bright, this suppository is bordering on zero to dermoreaction intensity, i.e. without skin allergic reaction.

Embodiment 4: long term toxicity test data

1, experimental technique

Random for Cavia porcellus part is become 4 groups, often group 15.In guinea pig back spinal column both sides, depilatory is uniform Coat so that it is depilation scope about 40 square centimeters.Clean depilatory, after treating 24 hours, often organize Cavia porcellus It is coated with suppository solution 0.2,0.4 and 0.8ml of the present invention respectively, respectively containing crude drug 92,184 and 368mg, Another group is coated with the every twice-daily of solvent 0.8ml, and continuous 30 days, the ordinary circumstance for the treatment of Cavia porcellus, experiment terminated Rear execution animal carries out hematology, blood biochemical and pathological examination.

2, result

Above-mentioned three groups of medication Cavia porcellus trunks go to hair-fields, have no that local skin has edema, hyperemia, erythema, goes out Blood point and ulcer.Medication group and control animals chroma of hair, ingest, the matched group such as extremity are movable is without bright Significant difference is different, blood biochemistry checking, and medication group and matched group are all in normal range.Histopathologic examination, Test each group of heart, liver, kidney and local skin and be showed no obvious pathological changes.Prompting, Chinese medicine suppository of the present invention is long Phase medication is showed no obvious toxic action to local skin and whole body important organ.

Result of the test shows: Chinese medicine suppository experiment safety of the present invention.This medicine is to group B streptococcus, golden yellow Staphylococcus, colibacillary minimal inhibitory concentration are 1: 150, and gonococcus is 1: 150, and white is read Pearl bacterium is 1: 300, and the time of killing during to 1: 20 dilution factor is 5 minutes, is 10 points when 1: 50 Clock, is 20 minutes when 1: 100, is 40 minutes when 1: 200, is 8 hours when 1: 400.

Clinical trial:

From in January ,-2016 in January, 2015, we are from intending tonifying kidney and nourishing yin side's treatment premature ovarian failure 50 Example, curative effect is preferable, is reported as follows.

1, clinical data

Totally 100 example, secondary amenorrhea, FSH rising person before being 40 years old.Age 17～39 Year, average (33.3 ± 6.8) year；Amenorrhea 1～11 years, average (3.6 ± 3.7) year.Amenorrhea it Before have hypomenorrhea, or irregular bleeding, or take place frequently or Hypomenorrhea, or amenorrhea suddenly.Wherein 3 The i.e. amenorrhea in example menophania 1～3 years, has 2 examples to take traditional Chinese medicine Radix Tripterygii Wilfordii history, and 1 example suffers from vitiligo clothes west Medicine (the most not quite clear)；Amenorrhea after 2～5 years, has 6 examples to have chronic pelvic inflammatory disease history.Its mother of 3 examples is had to exist Amenorrhea before 40 years old.There are 3 example secondary infertilities, 1 example primary infertility.All have hectic fever, perspiration, Anxiety, depression, insomnia or hypomnesis, or vaginal dryness, the disease such as sexual anhedonia or urine urgency-frequency Shape.100 examples are randomly divided into Chinese drug-treated group and Western medicine group.

Western medicine diagnose standard is to occur amenorrhea in more than at least 4 months before 40 years old, and have 2 times or with Upper serum FSH ＞ 40U/L (2 inspection intervals more than 1 month), estradiol level ＜ 73. 2pmol/L。

Tcm diagnosis standard is cracked ends before 40 years old, infrequent menstruation, gradually to amenorrhea or menolipsis suddenly, Warming sweating, hectic fever is flushing, dysphoria with feverish sensation in the chest palms and soles, dizziness and tinnitus, and soreness of the waist and knees or metapedes, with pain, are urinated red Dry stool, emaciated physique, vaginal dryness.B ultrasonic shows that uterus is less than normal or both sides ovary is less than normal, does not finds ovum The most developable little follicle of bubble or the continuously rarely seen only a few of monitoring, blood serum E2 low SI, FSH Significantly raised, red tongue or have crackle, tongue few, thready and rapid pulse or band string.

2 Therapeutic Method

Chinese drug-treated group tonifying kidney and nourishing yin side.Medicinal Radix Paeoniae Alba 12g, Caulis Spatholobi 13g, Herba Leonuri 12g, Rhizoma Cyperi 11g, Radix Arnebiae (Radix Lithospermi) 12g, Herba Patriniae 12g, Fructus Ligustri Lucidi 13g, Herba Ecliptae 12g, Fructus Mori 12g, Semen Cuscutae 12g, excessive sheep Leaves of pulse plants 12g, Cortex Moutan 13g, Radix Puerariae 12g, Radix Sophorae Flavescentis 11g, Fructus Cnidii 11g, Rhizoma Smilacis Glabrae 10g, Pseudobulbus Bletillae (Rhizoma Bletillae) 12g, Radix Angelicae Dahuricae 12g, Fructus Kochiae 13g, Herba Taraxaci 12g, Herba Menthae 13g, Herba Euphrasiae Regelii 12g, Radix Flemingiae Philippinensis 12g.Menstruation After clean, every day uses.Drug suppository of the present invention being removed cabinet, takes out stype, pull-out afterbody is cotton Line, is positioned over posterior fornix of vagina position, monthly 1, stops 24h every time, be used in conjunction 3 times.

Matched group gives synestrin tablets and is administered orally, and every day, 1mg, serve on 22d, rear 5d adapted Progesterone, every day 10 Mg intramuscular injection, is used in conjunction 3 menstrual cycle .2 group patients and uses mono blind method to carry out clinical trial, sees after 3 months Examine its month varied and serum sex hormone level .2 group patient equal acceptable learning, nutritious food medication while Adjust the comprehensive non-drug such as body constitution to process.Be used in conjunction 3 months is a course for the treatment of.

All treat statistical result after 1 course for the treatment of for two groups.

3 observational techniques

Clinical symptoms and menstrual cycle before and after treatment, through the change of amount, the change of Serum Sex Hormones, super Sound shows utero ovarian volume, and endometrium thickness changes.Blood FSH, E2 are by the court's epi chamber Inspection, uses luciferase linked immunosorbent assay, and reagent is produced by France bioMerieux, and color ultrasound is day This Nani17.Statistical counting data x²Inspection, measurement data t is checked.

4 criterions of therapeutical effect

Effective: clinical symptoms and sign disappear, Endocrinological inspection serum hormone is normal, menstrual onset and Continuous more than 2 times menorrheas, ovulation function recovers or gestation.Take a turn for the better: cardinal symptom and sign are bright Showing and alleviate, menstruation recovery is risen, but the cycle is abnormal, and Endocrinological inspection serum hormone takes a turn for the better or close Normally.Invalid: symptom, sign and Endocrinological inspection are not improved.

5 therapeutic outcomes

Two groups of comparitive study: two groups of comparitive study there was no significant difference (P ＞ 0.05).It is shown in Table 1.

1 liang of table group comparitive study example (%)

Group	n	Effective	Effectively	Invalid	Total effective rate
						Chinese drug-treated group	50	37	3	10	80%
Western medicine group	50	31	7	12	76%

Two groups of Serum Sex Hormones changes: serum E₂Significantly raised, FSH substantially reduces, two groups of treatments Front and back compare and have significant difference (P ＜ 0.05)；

Compare after two groups of treatments and there was no significant difference.

2 liang of table group Serum Sex Hormones Indexes Comparison (-x ± s)

After two groups of medications, untoward reaction is compared: the untoward reaction of Chinese drug-treated group is less than Western medicine group, and two groups are compared There is significant difference, (P ＜ 0.05), it is shown in Table 3.

Untoward reaction comparative example after the group medication of 3 liang of table

Group	Feel sick	Headache	Mammary swelling pain	Edema	Abdominal distention	Leucorrhea is many
							Chinese drug-treated group	2	1	0	0	1	2
Western medicine group	7	6	2	3	2	4

Two groups of symptoms improvement are compared: two groups of transference cure rates compare significant difference (P ＜ 0.05), It is shown in Table 4.

4 liang of table group symptom improves comparative example (%)

Premature ovarian failure accounts for the 1%～3.8% of women's total number of persons, is the normal of gynecological endocrine field See disease.Show as early sending out due to this type of patient or primary amenorrhea, estrogen are lowly caused encloses menopause Phase symptom and infertile, therefore belong to traditional Chinese medical science amenorrhea, menopause praecox, menopausal syndrome, infertile etc. Category.Owing to suffering from a deficiency of the kidney, function senilism is appointed in punching, and the dyspoiesis of menses causes uterus vessels of the uterus (sea of blood) Inanition, depletion of blood can descend and menopause praecox."Fu Qingzhu works on obstetric" cloud: " it is the most disconnected through water, Seem kidney water decline dry up ".Because women is based on blood, and " YIN often being in shortage, YANG often being in excess ", Therefore treatment should focus on tonifying kidney and nourishing yin, promoting blood flow to regulate menstruation.Radix Rehmanniae Preparata nourishing kidney-yin in tonifying kidney and nourishing yin Fang Fang, benefit Marrow is monarch drug.Fructus Corni, Radix Polygoni Multiflori, Semen Cuscutae liver and kidney tonifying, support essence and blood.Radix Arnebiae (Radix Lithospermi), life Pollen Typhae activating blood circulation to dissipate blood stasis, promoting the circulation of blood nourishing the liver blood, adjust punching to appoint.Herba Epimedii liver and kidney tonifying.Fructus Ligustri Lucidi, drought Lotus grass is ERZHI WAN, effect tonifying kidney and nourishing liver benefit essence.Radix Stellariae, Rhizoma Anemarrhenae nourishing YIN and clearing away heat pathogenic fire purging.Pueraria lobota Root clearing away heat and promoting production of body fluid, has estrogen-like effects according to modern pharmacological research Radix Puerariae, for women's reproduction Endocrine has regulation effect.All medicines share to be played tonifying kidney and nourishing yin altogether and invigorates blood circulation, and takes good care of punching and appoints effect.Mend Kidney regulating menstruation Chinese medicine has delay adenasthenia effect, has again promotion ovarian function recovery effect, Kidney-nourishing tcm drug has certain regulation effect to female gonad s function simultaneously, is ground by zoopery Studying carefully discovery kidney-reinforcing drug has promotion ovary LH/HCG receptor to increase phenomenon.Chinese medicine there may also be promotion FSH The effect that receptor produces, nourishing the liver and kidney, benefit intensive culture blood Chinese medicine have the effect promoting follicular development.With Kidney-reinforcing drug has estrogen-like to react with castration mouse, therefore thinks Chinese medicine scalable mankind's follicle Grow and ovulation.

Premature ovarian failure has obvious heterogeneity to clinical manifestation from the cause of disease to the course of disease, just carry out True etiological diagnosis, dialectical combines with differential diagnosis of diseases, takes corresponding treatment strategies, strong to improve Health or delay gonad old and feeble.Modern study is thought, Bushen Tiaochong Chinese medicine has health invigorating, regulation Gonad axis and immunologic function, even delay gonad aging effect.Clinic shows, tonifying kidney and nourishing yin side Safely, effectively, certainly, untoward reaction is few for treatment premature ovarian failure curative effect, is protecting in clinical practice Protecting, recover ovarian function aspect and have certain effect, its curative effect is equal to simple western medicine treatment, And toxic and side effects is less, low price.

Claims

1. the Chinese medicine suppository treating women's ovary senilism, it is characterised in that described Chinese medicine suppository bag Include the crude drug of following weight portion: the Radix Paeoniae Alba 10～20 parts, Caulis Spatholobi 10～20 parts, Herba Leonuri 10～20 parts, Rhizoma Cyperi 10～20 parts, Radix Arnebiae (Radix Lithospermi) 10～20 parts, Herba Patriniae 10～20 parts, Fructus Ligustri Lucidi 10～20 parts, Herba Ecliptae 10～20 parts, Fructus Mori 10～20 parts, Semen Cuscutae 10～20 parts, Herba Epimedii 10～20 parts, Cortex Moutan 10～20 Part, Radix Puerariae 10～20 parts, Radix Sophorae Flavescentis 10～20 parts, Fructus Cnidii 10～20 parts, Rhizoma Smilacis Glabrae 10～20 parts, in vain Splendid achnatherum 10～20 parts, the Radix Angelicae Dahuricae 10～20 parts, the Fructus Kochiae 10～20 parts, Herba Taraxaci 10～20 parts, Herba Menthae 10～ 20 parts, Herba Euphrasiae Regelii 10～20 parts, Radix Flemingiae Philippinensis 10～20 parts.

Treat the Chinese medicine suppository of women's ovary senilism the most according to claim 1, it is characterised in that institute State Chinese medicine suppository and also include the crude drug of following weight portion: the Radix Paeoniae Alba 10～15 parts, Caulis Spatholobi 10～15 parts, Herba Leonuri 10～15 parts, Rhizoma Cyperi 10～15 parts, Radix Arnebiae (Radix Lithospermi) 10～15 parts, Herba Patriniae 10～15 parts, Fructus Ligustri Lucidi 10～15 parts, Herba Ecliptae 10～15 parts, Fructus Mori 10～15 parts, Semen Cuscutae 10～15 parts, Herba Epimedii 10～ 15 parts, Cortex Moutan 10～15 parts, Radix Puerariae 10～20 parts, Radix Sophorae Flavescentis 10～20 parts, Fructus Cnidii 10～20 parts, soil Poria 10～20 parts, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～20 parts, the Radix Angelicae Dahuricae 10～20 parts, the Fructus Kochiae 10～20 parts, Herba Taraxaci 10～ 20 parts, Herba Menthae 10～20 parts, Herba Euphrasiae Regelii 10～20 parts, Radix Flemingiae Philippinensis 10～20 parts.

Treat the Chinese medicine suppository of women's ovary senilism the most according to claim 1, it is characterised in that institute State Chinese medicine suppository and also include the crude drug of following weight portion: the Radix Paeoniae Alba 10～20 parts, Caulis Spatholobi 10～20 parts, Herba Leonuri 10～20 parts, Rhizoma Cyperi 10～20 parts, Radix Arnebiae (Radix Lithospermi) 10～20 parts, Herba Patriniae 10～20 parts, Fructus Ligustri Lucidi 10～20 parts, Herba Ecliptae 10～20 parts, Fructus Mori 10～20 parts, Semen Cuscutae 10～20 parts, Herba Epimedii 10～ 20 parts, Cortex Moutan 10～20 parts, Radix Puerariae 10～15 parts, Radix Sophorae Flavescentis 10～15 parts, Fructus Cnidii 10～15 parts, soil Poria 10～15 parts, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～15 parts, the Radix Angelicae Dahuricae 10～15 parts, the Fructus Kochiae 10～15 parts, Herba Taraxaci 10～ 15 parts, Herba Menthae 10～15 parts, Herba Euphrasiae Regelii 10～15 parts, Radix Flemingiae Philippinensis 10～15 parts.

4. the Chinese medicine suppository for the treatment of women's ovary senilism as according to any one of claims 1 to 3 Preparation method, it is characterised in that the preparation process of described suppository includes:

B. remaining raw material ethanol is extracted, concentrate, as component 2；

The most according to claim 4, the preparation method of the Chinese medicine suppository for the treatment of women's ovary senilism, it is special Levy and be, in described step a, first the crude drug Pseudobulbus Bletillae (Rhizoma Bletillae), the Radix Angelicae Dahuricae, Herba Menthae steam method are distilled, To crystal, then pulverize, cross 100-120 mesh sieve, standby as component 1.

The most according to claim 4, the preparation method of the Chinese medicine suppository for the treatment of women's ovary senilism, it is special Levy and be, in described step b, then remaining crude drug is shredded, soaked and be placed in lid stainless steel cask In, adding 70% ethanol, quality is coarse grained 0.8-1 times, stirs, moistening airtight placement 1 hour Above, make fully to expand；Filter plate gauze bag bottom percolator is wrapped up after paving again by after moistening expansion Drug sample mix pine and do to dissipate, then contain powder with rustless steel spoon, load percolator uniformly, fill 10-12 Cm thick, even by T-bar pressure, then press aforesaid operations, and loading in layer, suitably pressurize, medicated powder Load 2/3 eminence that must not exceed percolator；Medicated powder face upper cover rustless steel orifice plate fastens, and opens under percolator The baiting valve in face, and put a container, then it is slowly added into 70% ethanol；Between medicated powder powder to be discharged Air, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, Then open baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material outflow per minute 2～3ml, Filtrate puts into liquid container, and pours ethanol during emptying into liquid container；Percolate is merged and stands, As component 2.

The most according to claim 4, the preparation method of the Chinese medicine suppository for the treatment of women's ovary senilism, it is special Levy and be, in described step c, component 1 component 2 is mixed into ointment；Choose substrate 50 DEG C～60 DEG C to add Heat is melted, and adds ointment cooling and shaping obtained above and makes class warhead shape suppository, and ultraviolet is killed virus.

8., according to the preparation method for the treatment of women's ovary senilism Chinese medicine suppository described in claim 4, it is special Levying and be, the substrate used by described suppository is selected from: semi-synthetic fatty acid glyceride, poloxamer, poly-second One or more in glycol, lanoline；Described substrate is heating and melting under the conditions of temperature 50 C～60 DEG C, Adding the medicine mastic that described preparation method prepares, stirring in water bath is uniform, is mixed into the liquid of thickness, fills Note, in plastic shape-forming pipe, inserts tampon, and cooling and shaping makes bullet-shaped suppository；Described tampon is tail Portion is with the tampon of cotton thread.

9. the Chinese medicine suppository for the treatment of women's ovary senilism as according to any one of claims 1 to 3 Preparation method, it is characterised in that the preparation process of described suppository also includes:

Treat the external used medicine of the Chinese medicine composition of women's ovary senilism the most according to claim 8 The preparation method of suppository, it is characterised in that in described step b, can put into crude drug in ethanol and add 2 extracting solution each 1～2 hour, are merged and stand by circumfluence distillation 2 times；By above two extracting solution Merge, the filtrate of 1.38 when concentrating under reduced pressure relative density is 70 DEG C, reclaim ethanol, gained filtrate is adjusted Proportion, to 1.60, is passed through spray dryer and is spray-dried, and crosses the compressed air of spray dryer shower nozzle Pressure is 4kg/m², hothouse hot stream temperature is 100 DEG C, obtains raw material medicated powder, as component 2.