CN106034708A - Cuttage propagation method for Acer saccharinum 'Laciniatum' - Google Patents
Cuttage propagation method for Acer saccharinum 'Laciniatum' Download PDFInfo
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- CN106034708A CN106034708A CN201610501975.1A CN201610501975A CN106034708A CN 106034708 A CN106034708 A CN 106034708A CN 201610501975 A CN201610501975 A CN 201610501975A CN 106034708 A CN106034708 A CN 106034708A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention relates to a cuttage propagation method for Acer saccharinum 'Laciniatum' and belongs to the technical field of plant propagation. The method comprises the following steps: (1) selecting an appropriate propagation place, and building a cutting orchard of maternal plants of the Acer saccharinum 'Laciniatum'; (2) loading a substrate to 72-hole trays; (3) carrying out cuttage once per year at appropriate cuttage time, wherein the appropriate cuttage time is the first ten days of September; (4) steeping cutting twigs by adopting a rooting promoter; (5) carrying out cuttage by adopting 72-hole trays in accordance with that one cutting is planted into each hole in a cuttage manner; and (6) starting to carry out seedling hardening 50 days after cuttage until about 75% of cuttings are rooted. The cuttage propagation method for the Acer saccharinum 'Laciniatum' can adapt to climate and soil environments of Chinese, the rooting condition is good, the rate of finished products reaches up to 90%, the operation is simple, and the propagation speed is high; and the method is high in adaptability, can be used for carrying out commercial large-scale production, is good in repeatability, is domestically reported for the first time and makes up vacancies in domestic related technical fields.
Description
Technical field
The invention belongs to technical field of plant propagation, specially spire Acer saccharum Marsh. cuttage breeding method.
Background technology
Spire Acer saccharum Marsh. Acer saccharinum ' Laciniatum ', Aceraceae, Acer L, broad leaved and deciduous broad leaved arbor, treelet
Bark is smooth, taupe, can become coarse after growing up, and like the skin of elephant, height of tree 12-24m, hat width is up to 9-15m, tree-like for ovum
Circle, tree vigo(u)r is grand, elegant.Dan Ye, to life, long 8-20cm, palmate 3-5 drastic crack, sliver is elongated, leaf green, and petiole is elongated,
Long 6-8cm, Autumn leaf colours can become yellow to golden yellow so that Chinese red, the most attractive in appearance.Corymb, light green, the florescence 4
Month, open before leaf launches, little brightly yellowish green.Band wing nut, green, it is transformed into brown after maturation October.Happiness nice and cool, moistening and
Slightly acidic environment fertile, well-drained, suitable pH value 5.5-7.3, the too low meeting of pH value causes fallen leaves.Happiness light, is resistant to certain
Sheltering from heat or light, tolerance to cold is strong, can tolerate-15 DEG C of low temperature.Spire Acer saccharum Marsh. originates in North America, and with Canada for many, 2004 by Zhejiang
Sen Hezhong industry limited company introduces from Holland, through safety, regionality, compatibility test, and uses different plant to urge
Root agent, variable concentrations, different substrates formula and the test of cottage method, it is thus achieved that success.Subject range: Shaanxi, Henan, Hebei,
Shandong, Anhui, Jiangsu, Zhejiang, Jiangxi, Hunan, Hubei, Sichuan, Guizhou, Yunnan.Spire Acer saccharum Marsh. blade in autumn is beautiful in colour, tree
It is preced with dense, is suitable for doing ornamental tree species in the bigger flower garden of area or open region, isolated planting or sheet can plant, as landscape tree, plant
Plant by garden, park, river, beach, there is higher ornamental value, be excellent large-scale garden landscape and beach Radix Saposhnikoviae tree
Kind.Consult existing publication and document, there is no the report that spire Acer saccharum Marsh. is relevant.
Summary of the invention
For the above-mentioned problems in the prior art, it is an object of the invention to design provides a kind of spire Acer saccharum Marsh. cuttage
The technical scheme of propagation method, comprises and sets up cutting orchard, and it is simple to operate, reproduction speed is fast, and yield rate is up to 90%.
Described spire Acer saccharum Marsh. cuttage breeding method, it is characterised in that comprise the following steps:
1) choosing suitable breeding spot and set up spire Acer saccharum Marsh. female parent cutting orchard, it is 40cm × 40cm that cutting orchard transplants seeding row spacing, close
Degree is for 2500-2800 plant/acre;
2) install substrate with 72 hole dishes, keep substrate elasticity consistent, first 24 hours of cuttage with 700-900 times of potassium permanganate
Solution is to substrate disinfection, and described substrate contains the following material of volume ratio: peat 20-30%, reed jasmine 15-25%, gold zone
Soil 10-15%, Maifanitum 5-15%, straw 8-15%, plant ash 15-25%, and by every cubic metre of cutting medium adds Bulbus Lycoridis Radiatae squama
Stem ground product 100-200g, ginkgo leaf powder minces 200-300g;
3) suitable cutting time is early September, cuttage in 1 year 1 time, and clip is selected in the spring tip and the intersection of autumn growth last year then, cuts
Become the cuttage branch of a length of 4-5cm, retain 2 lateral buds, 2 opposite leaf sheets, base portion flat nick;
4) cuttage branch uses and urges root agent 900-1100ppm dipping fringe bar base portion 2-3 second, described in urge root agent be 2-by weight ratio
The double gill GGR root-inducing powder of No. 6 of 4:3-5:1-3:0.5-1.5: naphthalene acetic acid: ABT1 root-inducing powder: Folium Ginkgo total flavones extracting solution
Mixing is constituted;
5) using 72 hole dishes to carry out cuttage, 1 fringe bar of every cave cuttage, the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately,
Spraying the carbendazim of 800-900 times of liquid after the airing of blade face, move-in-mud robot, in canopy, air humidity reaches more than 90%, at big ceiling
If sunshade net, above seedbed, set up two road sunshade nets, when air themperature is more than 35 DEG C in plastic sheeting, in two road sunshades
Side off the net carried out mist cooling, watered 1 water every 10-12 days;Such as fine day high temperature, from point at 11 in the morning to afternoon 3, Mei Geban
Hour spraying 1 time;
6) after cuttage 10 days, starting spray foliage fertilizer for the first time, later every 9-10, water and spray 1 time afterwards, foliage fertilizer can be with green
Fragrant 700-800 times of liquid of Wei sprays, simultaneously with 1000-1100 times of liquid Mancozeb spraying diseases prevention;After cuttage 50 days, about 75%
Fringe bar root of hair can start seedling exercising.
Described spire Acer saccharum Marsh. cuttage breeding method, it is characterised in that in step 1):
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed,
Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower;Pavement width 40cm, deeply
25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded;
Carrying out container seedling transplanting March in early November to next year, transplanting seeding row spacing is 40cm × 40cm, and density is 2600 plants/acre, fixed
Point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant, be compacted in root with fine earth heap during transplanting,
Irrigating in time and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth;
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later;In case of even
The continuous rainy day, timely draining;Full exposure is cultivated, and promotes fringe bar lignifying;Within after transplanting 15 days, starting fertilising, the 3-8 month is every half
The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time
Fertilizer, every mu of 1500kg, buriing of ditching.When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury
Wood stem, branch and blade are as the criterion;After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.
Described spire Acer saccharum Marsh. cuttage breeding method, it is characterised in that step 2) in:
The dry density 0.2-0.35 of described substrate, total porosity 85-90%, macroporsity 25-33%, aperture porosity 57-59%,
Maximum specific retention 5.0-5.4, pH value 5.7-6.0, EC value 0.5-0.6, full nitrogen 1.2-1.4%, full phosphorus 490-500mg/kg, full potassium
6300-6400mg/kg, calcium 2600-2700mg/kg, magnesium 2400-2500mg/kg, copper 5-6mg/kg, zinc 50-55mg/kg, boron
5.0-5.5mg/kg, ferrum 4600-4700mg/kg, manganese 110-120mg/kg, molybdenum 1.6-2.0mg/kg, aluminum 2600-2700mg/kg,
Lead 7-8mg/kg, sodium 3100-3200mg/kg, nickel 15-16mg/kg, sulfur 2400-2500mg/kg.
Described spire Acer saccharum Marsh. cuttage breeding method, it is characterised in that step 2) in: peat 25-28%, reed jasmine 18-20%,
Yellow soil 12-14%, Maifanitum 8-10%, straw 9-11%, plant ash 20-22%, and by every cubic metre of cutting medium adds stone
Bulbus Allii bulb ground product 150g, ginkgo leaf powder minces 250g.
Described spire Acer saccharum Marsh. cuttage breeding method, it is characterised in that step 2) described in cutting medium use with lower section
Prepared by method:
1) pretreatment: Bulbus Lycoridis Radiatae bulb, Folium Ginkgo are separately dried, are pulverized, prepared Bulbus Lycoridis Radiatae bulb ground product, ginkgo leaf powder mince;
2) the following material of percent by volume is mixed: peat 20-30%, reed jasmine 15-25%, yellow soil 10-15%, Maifanitum 5-
15%, straw 8-15%, plant ash 15-25%, and by every cubic metre of cutting medium adds Bulbus Lycoridis Radiatae bulb ground product 100-200g,
Ginkgo leaf powder minces 200-300g.
Described spire Acer saccharum Marsh. cuttage breeding method, it is characterised in that the Folium Ginkgo total flavones extracting solution described in step 4)
Prepared by employing following methods:
1) Folium Ginkgo pulverizing is broken into coarse powder, cross 20 mesh sieves;
2) seepage pressure effects: Folium Ginkgo coarse powder is loaded in percolator, after soaking 8 hours with 70% aquiferous ethanol, by 75% aqueous second
Alcohol carries out seepage pressure effects, if raw material weight is 1kg, the consumption of aquiferous ethanol is 8 liters, and flow velocity is 5mL/min, collects percolate;
3) flash concentration: by percolate bath temperature 70 DEG C, vacuum is 0.09 Mpa, and feed liquor flow velocity is 180mL min-1
Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 1.0g raw material;
4) macroporous resin adsorption purification: by the concentrated solution that obtains by macroporous adsorbent resin separation and concentration, successively with water, 10% contain
Water-ethanol, 25% aquiferous ethanol, 35% aquiferous ethanol, 45% aquiferous ethanol, 55% aquiferous ethanol, 70% aquiferous ethanol carry out gradient and wash
De-, finally with 70% acetone eluting, collect each position eluent of 3 times of column volumes respectively, collection merging, 25% aquiferous ethanol, 35%
Aquiferous ethanol, 45% aquiferous ethanol and the eluent at each position of 55% aquiferous ethanol, the eluent that will merge, obtain ginkgo biloba leaf total yellow
Ketone extracting solution.
Described spire Acer saccharum Marsh. cuttage breeding method, it is characterised in that in step 4): No. 6 double gill GGR root-inducing powders: naphthalene second
Acid: ABT1 root-inducing powder: the weight ratio of Folium Ginkgo total flavones extracting solution is 3:4:2:1.
Bulbus Lycoridis Radiatae (Lycoris radiata) belongs to Monocotyledonae Bulbus Lycoridis Radiatae (Amaryllidaceae), lycoris plants
Be used as medicinal plants very early to cure the disease and cure the wound, record according to Compendium of Material Medica etc.: Bulbus Lycoridis Radiatae not only have removing toxic substances, eliminate the phlegm, profit
Urine, the multiple efficacies such as emetic, it may also be used for laryngopharynx swelling and pain, carbuncle sore tumefacting virus, scrofula, edema etc..Lycorine is a kind of iloquinoline derivative
Alkaloid, for the derivant of pyridine in pyrroles's a pair of horses going side by side coffee.Research in recent years proves, lycorine have antiinflammatory, antiviral, malaria,
The protection multiple effect such as cardiovascular and inducing apoptosis of tumour cell.About the pharmacological action of medicinal ingredient in lycoris plants with
And the extraction and purification research of galantamine there are reports, but have no about the report for cutting medium.
Bulbus Lycoridis Radiatae bulb ground product in above-mentioned substrate, defending insect and killing is effective, and the raw material source of employing, in nature, belongs to and plants
Physical property insecticide, nontoxic to people, animal, and do not develop immunity to drugs, use safety.
Straw in above-mentioned substrate is pulverized 2-4cm respectively by Semen arachidis hypogaeae bar, bean bar, and adjustment water content 55-70%, C/N compare 30-
32, it is piled into ridge, with covered rearing with plastic film, makes the abundant fermentation maturity of straw, respectively obtain become thoroughly decomposed Semen arachidis hypogaeae bar and the bean bar that becomes thoroughly decomposed, will
Becoming thoroughly decomposed Semen arachidis hypogaeae bar and the ratio of the bean bar 2:1 that becomes thoroughly decomposed is mixed to prepare, have establishing in large scale in China, once, resource is rich for annual results
Rich.
Plant ash in above-mentioned substrate, be plant (draft and xylophyta) burning after ashes, so being all plants
Contained mineral element, nearly all contains in plant ash, and what wherein content was most is potassium element, general containing potassium 6 12%, wherein
90% is above water solublity, exists with carbonate form;Next to that phosphorus, general containing 1.5 3%;Possibly together with calcium, magnesium, silicon, sulfur and
The micronutrient elements such as ferrum, manganese, copper, zinc, boron, molybdenum, it is a kind of wide material sources, with low cost, nutrient is complete, fertilizer efficiency is obvious
Inorganic farm manure.
Maifanitum in above-mentioned substrate, belongs to volcanic rocks, and its main mine material is got angry mountain rock.Maifanitum is considered as
The lava that 5000-7550 ejects at 10000 years front volcanoes is embedded in underground, the high humidity through volcano, hot produced acidic materials with
And pressure produced by diastrophism and formed, Maifanitum originates in scabland.Maifanitum is a kind of natural medicine Ore, contains
There are 18 kinds of trace such as potassium necessary to human body, sodium, calcium, magnesium, phosphorus macroelement and zinc, ferrum, selenium, copper, strontium, iodine, fluorine, metasilicic acid
Element.
Containing substantial amounts of flavone compound in Folium Ginkgo, natural flavone compounds, without any chemosynthesis class thing
Matter, it can promote microorganism, plant cellular material operating and accumulation and cell enzyme activity, and then improve the generation of beneficial microbe bacterium
Thank to ability, increase the disease resistance of plant, promote plant growing.Ginkgo leaf powder in above-mentioned substrate minces, and can increase the anti-of plant
Characteristic of disease, promotes plant growing.Urge addition Folium Ginkgo total flavones extracting solution in root agent above-mentioned, the life of cuttage branch can be remarkably promoted
Root speed, and improve its disease resistance.
The cutting medium reasonable raw material proportioning that the present invention uses, has significant facilitation to cuttage shoot growth amount, also
Having the effect of defending insect and killing, can guarantee that again micronutrient element is complete, its simple and convenient processing method, production cost is low.
In the present invention, cutting orchard is set up and management, cutting time, cuttings length, cutting medium form and quality, urge root agent
Concentration and formula is different, yield rate is all had a significant impact by management after cuttage.
Described peat, reed jasmine, yellow soil, Maifanitum, straw, plant ash, Bulbus Lycoridis Radiatae bulb ground product, Folium Ginkgo are pulverized
Thing, No. 6 double gill GGR root-inducing powders, naphthalene acetic acid, ABT1 root-inducing powder, Folium Ginkgo total flavones extracting solution, green fragrant Wei, Mancozebs
All can the most directly buy.
Above-mentioned spire Acer saccharum Marsh. cuttage breeding method, adapts to the weather soil environment of China, takes root all right, yield rate
Up to 90%, simple to operate, reproduction speed is fast;The method strong adaptability, can be used for commercial scale metaplasia and produces, favorable repeatability,
For domestic reported first, fill up the blank of domestic correlative technology field.
The percentage composition related in present specification unless otherwise indicated, refers both to weight percentage.
Detailed description of the invention
In conjunction with embodiments of the invention, the invention will be further described.
1 cutting orchard is set up and management
1.1 selection of land
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed,
Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower;Pavement width 40cm, deeply
25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded.
1.2 transplant
March in early November to next year transplants.Graphic by seeding row spacing design configurations, transplanting seeding row spacing is 40cm × 40cm, close
Degree is 2600 plants/acre.Fixed point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant during transplanting, with thin
Mound is compacted in root, irrigates in time and determines root water, and bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth.
1.3 management
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later;In case of even
The continuous rainy day, timely draining;Full exposure is cultivated, and promotes fringe bar lignifying;Within after transplanting 15 days, starting fertilising, the 3-8 month is every half
The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time
Fertilizer, every mu of 1500kg, buriing of ditching.When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury
Wood stem, branch and blade are as the criterion;After adopting fringe, execute immediately 1 time quick-acting fat, every mu of 20kg.Weeding to accomplish " except early, except little, remove
", substantially without weeds on seedbed;After rain, fine day weeds with a hoe in time, keeps soil from packing together.
2 breed
2.1 cut fringe
Early September carries out cutting fringe, 1 year 1 time, and clip is selected in the spring tip and the intersection of autumn growth last year then, and cuttings length is
4.5cm, retains 2 lateral buds, 2 opposite leaf sheets, base portion flat nick.Retain female parent and cut fringe height 35cm.
2.2 material
Plastic greenhouse, overhead seedbed is used to carry out cuttage.Described substrate contains the following material of volume ratio: peat 20-30%, reed jasmine
15-25%, yellow soil 10-15%, Maifanitum 5-15%, straw 8-15%, plant ash 15-25%, and by every cubic metre of cutting medium
Adding Bulbus Lycoridis Radiatae bulb ground product 100-200g, ginkgo leaf powder minces 200-300g.
2.3 cuttages and management
Using booth overhead seedbed cuttage, seedbed width 168cm, bed surface floor file cloth, at ground cloth area mat substrate, stromal thickness
9cm, with 800 times of liquid disinfectants of potassium permanganate.
The double gill GGR root-inducing powder of use weight ratio to be 2-4:3-5:1-3:0.5-1.5 No. 6: naphthalene acetic acid: take root for No. ABT1
Powder: Folium Ginkgo total flavones extracting solution 500ppm, quickly dipped fringe bar base portion 2 seconds, cuttage seeding row spacing is 8cm × 12cm, the cuttage degree of depth
3cm;;Water permeable after cuttage immediately, after blade face is dried, spray the carbendazim of 1000 times of liquid, then cover plastic sheeting, use bamboo
Sheet is inserted into Small plastic shed, sealing film surrounding, and in making canopy, air humidity reaches more than 95%.Sunshade net is set, on seedbed at big ceiling
Side (at high 2m) sets up two road sunshade nets, when in plastic sheeting, air themperature is more than 35 DEG C, enters below two road sunshade nets
Row mist cooling.Such as fine day high temperature, from point at 10 in the morning to afternoon 4, spray 1 time every half an hour.Check that substrate is wet every day
Degree, can not overly moist, also can not overdrying, when the thin film reverse side full little water droplet of knot, in Small plastic shed, relative air humidity is more than 95%, no
With keeping the skin wet;When little water droplet is little, water supply in media is not enough, between the lights or cloudy day, open thin film, water permeable, then stand
Will seal in film cover, typically water 1 water every 7-10 days.
2.4 seedling exercising
After cuttage about 50 days, the fringe bar root of hair of about 75% can start seedling exercising.First open thin film two to ventilate 2 days, then exist
Dusk or cloudy day all open;Can manage with full exposure after 2 days at Small plastic shed upper cover last layer sunshade net after opening;
After cuttage 10 days, start spray foliage fertilizer for the first time, later every 7-10 days, water and spray 1 time afterwards.Foliage fertilizer can be with green fragrant Wei
1000 times of liquid, or 0.2% carbamide sprays or waters and execute.In conjunction with diseases prevention, with 1000 times of liquid Mancozeb sprayings.
4 commercial seedlings make
4.1 seedbed Seedlings
By in the substrate in overhead for tloig-cutlage to warmhouse booth seedbed, through 8 months take pains to foster, form seedbed Seedling.Seedling is advised
Lattice: the long 5cm of young sprout, well developed root system, robust plant, without pest and disease damage, yield rate 85%.
4.2 ground plant Seedling
Seedbed Seedling is moved into big field, took pains to foster through 18 months, form ground and plant Seedling.Seedling specification: height of seedling 120cm, is preced with width
60cm, robust plant, without pest and disease damage, yield rate 90%.
Claims (7)
1. spire Acer saccharum Marsh. cuttage breeding method, it is characterised in that comprise the following steps:
1) choosing suitable breeding spot and set up spire Acer saccharum Marsh. female parent cutting orchard, it is 40cm × 40cm that cutting orchard transplants seeding row spacing, close
Degree is for 2500-2800 plant/acre;
2) install substrate with 72 hole dishes, keep substrate elasticity consistent, first 24 hours of cuttage with 700-900 times of potassium permanganate
Solution is to substrate disinfection, and described substrate contains the following material of volume ratio: peat 20-30%, reed jasmine 15-25%, gold zone
Soil 10-15%, Maifanitum 5-15%, straw 8-15%, plant ash 15-25%, and by every cubic metre of cutting medium adds Bulbus Lycoridis Radiatae squama
Stem ground product 100-200g, ginkgo leaf powder minces 200-300g;
3) suitable cutting time is early September, cuttage in 1 year 1 time, and clip is selected in the spring tip and the intersection of autumn growth last year then, cuts
Become the cuttage branch of a length of 4-5cm, retain 2 lateral buds, 2 opposite leaf sheets, base portion flat nick;
4) cuttage branch uses and urges root agent 900-1100ppm dipping fringe bar base portion 2-3 second, described in urge root agent be 2-by weight ratio
The double gill GGR root-inducing powder of No. 6 of 4:3-5:1-3:0.5-1.5: naphthalene acetic acid: ABT1 root-inducing powder: Folium Ginkgo total flavones extracting solution
Mixing is constituted;
5) using 72 hole dishes to carry out cuttage, 1 fringe bar of every cave cuttage, the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately,
Spraying the carbendazim of 800-900 times of liquid after the airing of blade face, move-in-mud robot, in canopy, air humidity reaches more than 90%, at big ceiling
If sunshade net, above seedbed, set up two road sunshade nets, when air themperature is more than 35 DEG C in plastic sheeting, in two road sunshades
Side off the net carried out mist cooling, watered 1 water every 10-12 days;Such as fine day high temperature, from point at 11 in the morning to afternoon 3, Mei Geban
Hour spraying 1 time;
6) after cuttage 10 days, starting spray foliage fertilizer for the first time, later every 9-10, water and spray 1 time afterwards, foliage fertilizer can be with green
Fragrant 700-800 times of liquid of Wei sprays, simultaneously with 1000-1100 times of liquid Mancozeb spraying diseases prevention;After cuttage 50 days, about 75%
Fringe bar root of hair can start seedling exercising.
2. spire Acer saccharum Marsh. cuttage breeding method as claimed in claim 1, it is characterised in that in step 1):
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed,
Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower;Pavement width 40cm, deeply
25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded;
Carrying out container seedling transplanting March in early November to next year, transplanting seeding row spacing is 40cm × 40cm, and density is 2600 plants/acre, fixed
Point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant, be compacted in root with fine earth heap during transplanting,
Irrigating in time and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth;
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later;In case of even
The continuous rainy day, timely draining;Full exposure is cultivated, and promotes fringe bar lignifying;Within after transplanting 15 days, starting fertilising, the 3-8 month is every half
The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time
Fertilizer, every mu of 1500kg, buriing of ditching;When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury
Wood stem, branch and blade are as the criterion;After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.
3. spire Acer saccharum Marsh. cuttage breeding method as claimed in claim 1, it is characterised in that step 2) in:
The dry density 0.2-0.35 of described substrate, total porosity 85-90%, macroporsity 25-33%, aperture porosity 57-59%,
Maximum specific retention 5.0-5.4, pH value 5.7-6.0, EC value 0.5-0.6, full nitrogen 1.2-1.4%, full phosphorus 490-500mg/kg, full potassium
6300-6400mg/kg, calcium 2600-2700mg/kg, magnesium 2400-2500mg/kg, copper 5-6mg/kg, zinc 50-55mg/kg, boron
5.0-5.5mg/kg, ferrum 4600-4700mg/kg, manganese 110-120mg/kg, molybdenum 1.6-2.0mg/kg, aluminum 2600-2700mg/kg,
Lead 7-8mg/kg, sodium 3100-3200mg/kg, nickel 15-16mg/kg, sulfur 2400-2500mg/kg.
4. spire Acer saccharum Marsh. cuttage breeding method as claimed in claim 1, it is characterised in that step 2) in: peat 25-28%, reed
Jasmine 18-20%, yellow soil 12-14%, Maifanitum 8-10%, straw 9-11%, plant ash 20-22%, and by every cubic metre of cutting medium
Middle interpolation Bulbus Lycoridis Radiatae bulb ground product 150g, ginkgo leaf powder minces 250g.
5. spire Acer saccharum Marsh. cuttage breeding method as claimed in claim 1, it is characterised in that step 2) described in cutting medium
Prepared by employing following methods:
1) pretreatment: Bulbus Lycoridis Radiatae bulb, Folium Ginkgo are separately dried, are pulverized, prepared Bulbus Lycoridis Radiatae bulb ground product, ginkgo leaf powder mince;
2) the following material of percent by volume is mixed: peat 20-30%, reed jasmine 15-25%, yellow soil 10-15%, Maifanitum 5-
15%, straw 8-15%, plant ash 15-25%, and by every cubic metre of cutting medium adds Bulbus Lycoridis Radiatae bulb ground product 100-200g,
Ginkgo leaf powder minces 200-300g.
6. spire Acer saccharum Marsh. cuttage breeding method as claimed in claim 1, it is characterised in that the ginkgo biloba leaf total described in step 4)
Flavone extractive uses following methods to prepare:
1) Folium Ginkgo pulverizing is broken into coarse powder, cross 20 mesh sieves;
2) seepage pressure effects: Folium Ginkgo coarse powder is loaded in percolator, after soaking 8 hours with 70% aquiferous ethanol, by 75% aqueous second
Alcohol carries out seepage pressure effects, if raw material weight is 1kg, the consumption of aquiferous ethanol is 8 liters, and flow velocity is 5mL/min, collects percolate;
3) flash concentration: by percolate bath temperature 70 DEG C, vacuum is 0.09 Mpa, and feed liquor flow velocity is 180mL min-1
Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 1.0g raw material;
4) macroporous resin adsorption purification: by the concentrated solution that obtains by macroporous adsorbent resin separation and concentration, successively with water, 10% contain
Water-ethanol, 25% aquiferous ethanol, 35% aquiferous ethanol, 45% aquiferous ethanol, 55% aquiferous ethanol, 70% aquiferous ethanol carry out gradient and wash
De-, finally with 70% acetone eluting, collect each position eluent of 3 times of column volumes respectively, collection merging, 25% aquiferous ethanol, 35%
Aquiferous ethanol, 45% aquiferous ethanol and the eluent at each position of 55% aquiferous ethanol, the eluent that will merge, obtain ginkgo biloba leaf total yellow
Ketone extracting solution.
7. spire Acer saccharum Marsh. cuttage breeding method as claimed in claim 1, it is characterised in that in step 4): No. 6 double gill GGR are raw
Root powder: naphthalene acetic acid: ABT1 root-inducing powder: the weight ratio of Folium Ginkgo total flavones extracting solution is 3:4:2:1.
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