CN105993821A - Propagation method for grape by grafting tender branches on hard branch used as rootstock - Google Patents
Propagation method for grape by grafting tender branches on hard branch used as rootstock Download PDFInfo
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- CN105993821A CN105993821A CN201610340733.9A CN201610340733A CN105993821A CN 105993821 A CN105993821 A CN 105993821A CN 201610340733 A CN201610340733 A CN 201610340733A CN 105993821 A CN105993821 A CN 105993821A
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- branch
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- anvil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/02—Cultivation of hops or vines
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Abstract
The invention discloses a propagation method for grape by grafting tender branches on a hard branch used as a rootstock, belonging to the field of plant propagation. The propagation method comprises the following steps: selection and storage of scions, selection and storage of the rootstock, scion treatment, rootstock treatment, cuttage grafting of scion and rootstock branches, and healing promotion of graft unions, wherein sandy soil used for storage of the rootstock in the step 2 can be sterilized by using a thiophanate methyl solution; the cutting faces under the scions are dipped with indoleacetic acid to promote healing; and the scion and rootstock branches are coated with the indoleacetic acid to promote better rooting effect after cuttage grafting. The propagation method provided by the invention has the advantages of high survival rate, simple operation, low cost, and applicability to general application.
Description
Technical field
The present invention relates to Fructus Vitis viniferae propagation method, be especially a kind of vine hard branch anvil and connect the reproduction technique of twig, belong to field of plant reproduction.
Background technology
Fructus Vitis viniferae grafting cultivation is the vinegrowing mode currently generally used, be improve that Fructus Vitis viniferae is disease-resistant, drought resisting, the effective measures of the ability such as cold-resistant and insect pest, the most again can with improving quality, improve yield, reduce production cost, expand growing area.Grafting between vine hard branch and twig is one of engrafting method of current application, and mainly with twig as stock, with hard branch for scion grafting, owing to stock growing way is weak, very thin, and scion growing way is prosperous, easily causes rugosity between fringe anvil inconsistent, and fringe is coarser than anvil.The present invention attempts to overcome disadvantage mentioned above, attempt with a kind of hard branch be stock, twig be the engrafting method of scion.
Summary of the invention
The technical problem to be solved is to overcome prior art defect, it is provided that a kind of vine hard branch anvil connects the reproduction technique of twig.
The technical scheme is that
The selection of first step scion and storage: selecting a diameter of 0.7～0.9cm thick, robust growth, semi-lignified young sprout without pest and disease damage, every 1～3 buds are cut into one, are placed in clear water standby；
The selection of second step stock and storage: choose the grape variety being harmonious with scion, after Fructus Vitis viniferae leaf-fall, cut in conjunction with the winter, clip robust growth, without pest and disease damage, sprout is full, Maturity good, about 0.7～the thick desire grafting twig of 0.9cm, arrange and be placed on temperature-5～5。The sandy soil of C, humidity 5～7% are preserved, keeps ventilating；
The process of the 3rd step scion: branch selected by shearing procedure one, makes clip away from bud 2cm straight snips, and lower clip is away from bud 0.5cm wedge shape, and is inwardly cut along leaf margin by upper bud-leaf sheet and stay 1/4～1/2, is placed in clear water standby；
The process of the 4th step stock: the branch that the winter hides is cut into 60～70cm length, it is placed in water cleaning clothing and soaks 12～24 hours, it is that emerald green taking-up is dried to clip, branch after drying is cut into 12～18cm long shoot sections and rejects eye, branch section upper and lower side cuts flat, under cross section, branch section upper end central axis is split, deep 2～3cm, standby；
5th step fringe anvil stem cutting connects: choosing the suitable scion of thickness and the pairing of anvil branch, what scion inserted stock splits in seam, make cambium layer align, anvil branch clamping cladus, with plastic sheeting upwards binding grafting mouth at end opening 1～2cm, until cladus mouth will be sealed, outside cladus eye is exposed to；
6th step interface promoting healing: will transfer and be placed in chest by branch, sandy soil cover, are placed in hot house, and temperature of shed is maintained at 18～29。C, waters once in every 4 days, transplants after interface organization healing.
Further, in step 2, the sandy soil of storage scion and stock dry with sterilization after irrigating with 550～750 times of thiophanate methyl solution in advance.
Further, under scion, bevel dips in 120～150ppm heteroauxing promoting healings.
Further, 280～360ppm heteroauxing growth-promoting roots it are coated with after the stem cutting of fringe anvil connects.
Beneficial effects of the present invention: the present invention is greatly improved the survival rate of Fructus Vitis viniferae, it is simple to operate, and reproductive-cost is low, it is simple to popularization and application.
Detailed description of the invention
Below in conjunction with embodiment, the preparation method of the present invention is described further.
[embodiment one]
The selection of first step scion and storage: select that a diameter of 0.7cm is thick, robust growth, semi-lignified young sprout without pest and disease damage, every 1 bud is cut into one, is placed in clear water standby；
The selection of second step stock and storage: choose the grape variety being harmonious with scion, after Fructus Vitis viniferae leaf-fall, cut in conjunction with the winter, clip robust growth, without pest and disease damage, sprout is full, Maturity good, about 0.7cm is thick desire grafting twig, arranges and is placed on temperature-55。C, humidity 5% sandy soil in preserve, sandy soil dry after irrigating with 550 times of thiophanate methyl solution in advance with sterilization, keep ventilation；
The process of the 3rd step scion: branch selected by shearing procedure one, making clip away from bud 2cm straight snips, lower clip is away from bud 0.5cm wedge shape, and is inwardly cut along leaf margin by upper bud-leaf sheet and stay 1/4, being placed in clear water standby, during grafting, under scion, bevel dips in 120ppm heteroauxing promoting healing；
The process of the 4th step stock: the branch that the winter hides is cut into 60cm length, it is placed in water cleaning clothing and soaks 12 hours, it is that emerald green taking-up is dried to clip, branch after drying is cut into 12cm long shoot section and rejects eye, branch section upper and lower side cuts flat, under cross section, branch section upper end central axis is split, and deep 2cm,, standby；
5th step fringe anvil stem cutting connects: choose the suitable scion of thickness and the pairing of anvil branch, what scion inserted stock splits in seam, cambium layer is made to align, anvil branch clamping cladus, with plastic sheeting upwards binding grafting mouth at end opening 1cm, until cladus mouth will be sealed, outside cladus eye is exposed to, fringe anvil stem cutting is coated with 280ppm heteroauxing growth-promoting root at anvil branch end opening after connecing；
6th step interface promoting healing: will transfer and be placed in chest by branch, sandy soil cover, are placed in hot house, and temperature of shed is maintained at 18。C, waters once in every 4 days, transplants after interface organization healing.
[embodiment two]
The selection of first step scion and storage: select that a diameter of 0.9cm is thick, robust growth, semi-lignified young sprout without pest and disease damage, every 3 buds are cut into one, are placed in clear water standby；
The selection of second step stock and storage: choose the grape variety being harmonious with scion, after Fructus Vitis viniferae leaf-fall, cut in conjunction with the winter, clip robust growth, without pest and disease damage, sprout is full, Maturity good, about 0.9cm is thick desire grafting twig, arranges and is placed on temperature 5。C, humidity 7% sandy soil in preserve, sandy soil dry after irrigating with 750 times of thiophanate methyl solution in advance with sterilization, keep ventilation；
The process of the 3rd step scion: branch selected by shearing procedure one, making clip away from bud 2cm straight snips, lower clip is away from bud 0.5cm wedge shape, and is inwardly cut along leaf margin by upper bud-leaf sheet and stay 1/2, being placed in clear water standby, during grafting, under scion, bevel dips in 150ppm heteroauxing promoting healing；
The process of the 4th step stock: the branch that the winter hides is cut into 70cm length, it is placed in water cleaning clothing and soaks 24 hours, it is that emerald green taking-up is dried to clip, branch after drying is cut into 18cm long shoot section and rejects eye, branch section upper and lower side cuts flat, under cross section, branch section upper end central axis is split, deep 3cm, standby；
5th step fringe anvil stem cutting connects: choose the suitable scion of thickness and the pairing of anvil branch, what scion inserted stock splits in seam, cambium layer is made to align, anvil branch clamping cladus, with plastic sheeting upwards binding grafting mouth at end opening 2cm, until cladus mouth will be sealed, outside cladus eye is exposed to, fringe anvil stem cutting is coated with 360ppm heteroauxing growth-promoting root at anvil branch end opening after connecing；
6th step interface promoting healing: will transfer and be placed in chest by branch, sandy soil cover, are placed in hot house, and temperature of shed is maintained at 29。C, waters once in every 4 days, transplants after interface organization healing.
[embodiment three]
The selection of first step scion and storage: select that a diameter of 0.8cm is thick, robust growth, semi-lignified young sprout without pest and disease damage, every 2 buds are cut into one, are placed in clear water standby；
The selection of second step stock and storage: choose the grape variety being harmonious with scion, after Fructus Vitis viniferae leaf-fall, cut in conjunction with the winter, clip robust growth, without pest and disease damage, sprout is full, Maturity good, about 0.8cm is thick desire grafting twig, arranges and is placed on temperature 0。C, humidity 6% sandy soil in preserve, sandy soil dry after irrigating with 650 times of thiophanate methyl solution in advance with sterilization, keep ventilation；
The process of the 3rd step scion: branch selected by shearing procedure one, making clip away from bud 2cm straight snips, lower clip is away from bud 0.5cm wedge shape, and is inwardly cut along leaf margin by upper bud-leaf sheet and stay 1/3, being placed in clear water standby, during grafting, under scion, bevel dips in 135ppm heteroauxing promoting healing；
The process of the 4th step stock: the branch that the winter hides is cut into 65cm length, it is placed in water cleaning clothing and soaks 18 hours, it is that emerald green taking-up is dried to clip, branch after drying is cut into 15cm long shoot section and rejects eye, branch section upper and lower side cuts flat, under cross section, branch section upper end central axis is split, deep 2.5cm, standby；
5th step fringe anvil stem cutting connects: choose the suitable scion of thickness and the pairing of anvil branch, what scion inserted stock splits in seam, cambium layer is made to align, anvil branch clamping cladus, with plastic sheeting upwards binding grafting mouth at end opening 1.5cm, until cladus mouth will be sealed, outside cladus eye is exposed to, fringe anvil stem cutting is coated with 320ppm heteroauxing growth-promoting root at anvil branch end opening after connecing；
6th step interface promoting healing: will transfer and be placed in chest by branch, sandy soil cover, are placed in hot house, and temperature of shed is maintained at 25。C, waters once in every 4 days, transplants after interface organization healing.
Above the detailed description of the invention of the present invention is described, but the present invention is not limited to above description.For a person skilled in the art, any equal amendment to the technical program and replacement are all within the scope of the invention.Therefore, the impartial conversion made without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (8)
1. a vine hard branch anvil connects the propagation method of twig, it is characterised in that comprise the following steps:
The selection of first step scion and storage: selecting a diameter of 0.7～0.9cm thick, robust growth, semi-lignified young sprout without pest and disease damage, every 1～3 buds are cut into one, are placed in clear water standby；
The selection of second step stock and storage: choose the grape variety being harmonious with scion, after Fructus Vitis viniferae leaf-fall, cut in conjunction with the winter, clip robust growth, without pest and disease damage, sprout is full, Maturity good, about 0.7～the thick desire grafting twig of 0.9cm, arrange to be placed in the sandy soil of temperature-5～5 DEG C, humidity 5～7% and preserve, keep ventilating；
The process of the 3rd step scion: branch selected by shearing procedure one, makes clip away from bud 2cm straight snips, and lower clip is away from bud 0.5cm wedge shape, and is inwardly cut along leaf margin by upper bud-leaf sheet and stay 1/4～1/2, is placed in clear water standby；
The process of the 4th step stock: the branch that the winter hides is cut into 60～70cm length, it is placed in water cleaning clothing and soaks 12～24 hours, it is that emerald green taking-up is dried to clip, branch after drying is cut into 12～18cm long shoot sections and rejects eye, branch section upper and lower side cuts flat, under cross section, branch section upper end central axis is split, deep 2～3cm is standby；
5th step fringe anvil stem cutting connects: choosing the suitable scion of thickness and the pairing of anvil branch, what scion inserted stock splits in seam, make cambium layer align, anvil branch clamping cladus, with plastic sheeting upwards binding grafting mouth at end opening 1～2cm, until cladus mouth will be sealed, outside cladus eye is exposed to；
6th step interface promoting healing: will transfer and be placed in chest by branch, sandy soil cover, are placed in hot house, and temperature of shed is maintained at 18～29 DEG C, within every 4 days, waters once, transplant after interface organization healing.
Vine hard branch anvil the most according to claim 1 connects the propagation method of twig, it is characterised in that said method comprising the steps of:
The selection of first step scion and storage: select that a diameter of 0.8cm is thick, robust growth, semi-lignified young sprout without pest and disease damage, every 2 buds are cut into one, are placed in clear water standby；
The selection of second step stock and storage: choose the grape variety being harmonious with scion, after Fructus Vitis viniferae leaf-fall, cut in conjunction with the winter, clip robust growth, without pest and disease damage, sprout is full, Maturity good, about 0.8cm is thick desire grafting twig, arrange to be placed in the sandy soil of temperature 0 DEG C, humidity 6% and preserve, keep ventilating；
The process of the 3rd step scion: branch selected by shearing procedure one, makes clip away from bud 2cm straight snips, and lower clip is away from bud 0.5cm wedge shape, and is inwardly cut along leaf margin by upper bud-leaf sheet and stay 1/3, is placed in clear water standby；
The process of the 4th step stock: the branch that the winter hides is cut into 65cm length, it is placed in water cleaning clothing and soaks 18 hours, it is that emerald green taking-up is dried to clip, branch after drying is cut into 15cm long shoot section and rejects eye, branch section upper and lower side cuts flat, under cross section, branch section upper end central axis is split, deep 2.5cm, standby；
5th step fringe anvil stem cutting connects: choosing the suitable scion of thickness and the pairing of anvil branch, what scion inserted stock splits in seam, make cambium layer align, anvil branch clamping cladus, with plastic sheeting upwards binding grafting mouth at end opening 1.5cm, until cladus mouth will be sealed, outside cladus eye is exposed to；
6th step interface promoting healing: will transfer and be placed in chest by branch, sandy soil cover, are placed in hot house, and temperature of shed is maintained at 25 DEG C, within every 4 days, waters once, transplant after interface organization healing.
Vine hard branch anvil the most according to claim 1 and 2 connects the propagation method of twig, it is characterised in that: step one, the sandy soil preserving scion and stock in two sterilize with antibacterial in advance, dry standby after irrigating.
Fructus Vitis viniferae the most according to claim 3 in vitro stiff wood grafting propagation method, it is characterised in that: described antibacterial is 550～750 times of thiophanate methyl solution.
Vine hard branch anvil the most according to claim 1 and 2 connects the propagation method of twig, it is characterised in that: under described scion, bevel dips in consolidant promoting healing.
Vine hard branch anvil the most according to claim 5 connects the propagation method of twig, it is characterised in that: described consolidant is 120～150ppm heteroauxings.
Vine hard branch anvil the most according to claim 1 and 2 connects the propagation method of twig, it is characterised in that: described fringe anvil stem cutting is coated with root-growing agent growth-promoting root after connecing.
Vine hard branch anvil the most according to claim 7 connects the propagation method of twig, it is characterised in that: described root-growing agent is 280～360ppm heteroauxings.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610340733.9A CN105993821A (en) | 2016-05-23 | 2016-05-23 | Propagation method for grape by grafting tender branches on hard branch used as rootstock |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610340733.9A CN105993821A (en) | 2016-05-23 | 2016-05-23 | Propagation method for grape by grafting tender branches on hard branch used as rootstock |
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| CN105993821A true CN105993821A (en) | 2016-10-12 |
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| CN201610340733.9A Pending CN105993821A (en) | 2016-05-23 | 2016-05-23 | Propagation method for grape by grafting tender branches on hard branch used as rootstock |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107667764A (en) * | 2017-08-23 | 2018-02-09 | 成都圻坊生物科技有限公司 | A kind of grape engrafting method for being advantageous to volume increase |
| CN108391550A (en) * | 2018-02-11 | 2018-08-14 | 荔浦县万家兴果蔬专业合作社 | A kind of method for culturing seedlings of shatian pomelo |
| CN108605548A (en) * | 2016-12-23 | 2018-10-02 | 句容市绿润苗木有限公司 | Vine hard branch anvil connects the propagation method of spray |
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| CN1439250A (en) * | 2002-12-16 | 2003-09-03 | 穆德春 | Method for grafting hard grape scion onto green stock |
| CN101953274A (en) * | 2010-10-27 | 2011-01-26 | 河北省农林科学院昌黎果树研究所 | Method for performing high position grafting on grapes by using multiresistant rootstocks |
| CN104041331A (en) * | 2013-03-15 | 2014-09-17 | 天津明珠葡萄种植专业合作社 | Grafting method for carrying out stock grafting on grape greenwood and grape stiff wood |
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2016
- 2016-05-23 CN CN201610340733.9A patent/CN105993821A/en active Pending
Patent Citations (3)
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| CN1439250A (en) * | 2002-12-16 | 2003-09-03 | 穆德春 | Method for grafting hard grape scion onto green stock |
| CN101953274A (en) * | 2010-10-27 | 2011-01-26 | 河北省农林科学院昌黎果树研究所 | Method for performing high position grafting on grapes by using multiresistant rootstocks |
| CN104041331A (en) * | 2013-03-15 | 2014-09-17 | 天津明珠葡萄种植专业合作社 | Grafting method for carrying out stock grafting on grape greenwood and grape stiff wood |
Non-Patent Citations (3)
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| 孙海生等: "葡萄硬枝离体嫁接试验", 《果农之友》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108605548A (en) * | 2016-12-23 | 2018-10-02 | 句容市绿润苗木有限公司 | Vine hard branch anvil connects the propagation method of spray |
| CN107667764A (en) * | 2017-08-23 | 2018-02-09 | 成都圻坊生物科技有限公司 | A kind of grape engrafting method for being advantageous to volume increase |
| CN108391550A (en) * | 2018-02-11 | 2018-08-14 | 荔浦县万家兴果蔬专业合作社 | A kind of method for culturing seedlings of shatian pomelo |
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