CN105950640A - 以海洋细菌为来源获取的κ-卡拉胶酶基因及重组酶制备方法 - Google Patents
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- CN105950640A CN105950640A CN201610503827.3A CN201610503827A CN105950640A CN 105950640 A CN105950640 A CN 105950640A CN 201610503827 A CN201610503827 A CN 201610503827A CN 105950640 A CN105950640 A CN 105950640A
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- C—CHEMISTRY; METALLURGY
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C12Y—ENZYMES
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- C12Y302/01083—Kappa-carrageenase (3.2.1.83)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2800/00—Nucleic acids vectors
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Abstract
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CN201610503827.3A CN105950640B (zh) | 2016-06-30 | 2016-06-30 | 以海洋细菌为来源获取的κ-卡拉胶酶基因及重组酶制备方法 |
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CN201610503827.3A CN105950640B (zh) | 2016-06-30 | 2016-06-30 | 以海洋细菌为来源获取的κ-卡拉胶酶基因及重组酶制备方法 |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106566837A (zh) * | 2016-11-22 | 2017-04-19 | 大连大学 | 一种海洋细菌来源ι‑卡拉胶酶基因及其应用 |
CN106755190A (zh) * | 2016-11-22 | 2017-05-31 | 大连大学 | 基于重组κ‑卡拉胶酶制备卡拉胶寡糖的方法 |
CN109762799A (zh) * | 2018-12-26 | 2019-05-17 | 青岛大学 | 一种κ-卡拉胶酶及其应用 |
CN109929859A (zh) * | 2017-12-15 | 2019-06-25 | 中国科学院大连化学物理研究所 | 一种κ-卡拉胶酶编码基因及其制备与应用 |
CN110551716A (zh) * | 2019-09-10 | 2019-12-10 | 集美大学 | 基因Glu525的制备方法和用途 |
CN111303310A (zh) * | 2020-03-12 | 2020-06-19 | 大连大学 | 一种海洋硫酸半乳六糖的制备方法和其在抗阿尔兹海默病药物制备中的应用 |
CN115725548A (zh) * | 2022-10-26 | 2023-03-03 | 集美大学 | 一种κ-卡拉胶酶Pcar16突变体及其制备方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105018544A (zh) * | 2015-08-07 | 2015-11-04 | 大连大学 | 具有促进海参生长和抗逆作用的卡拉胶寡糖及其制法和应用 |
-
2016
- 2016-06-30 CN CN201610503827.3A patent/CN105950640B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105018544A (zh) * | 2015-08-07 | 2015-11-04 | 大连大学 | 具有促进海参生长和抗逆作用的卡拉胶寡糖及其制法和应用 |
Non-Patent Citations (2)
Title |
---|
ASAHINA A.Y.等: "Cellulophaga lytica strain HI1,complete genome", 《NCBI-GENBANK》 * |
ZHEMIN LIU等: "Molecular cloning,characterization,and heterologous expression of a new κ-carrageenase gene from marine bacterium Zobellia sp. ZM-2", 《APPLIED MICROBIOLOGY& BIOTECHNOLOGY》 * |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106566837A (zh) * | 2016-11-22 | 2017-04-19 | 大连大学 | 一种海洋细菌来源ι‑卡拉胶酶基因及其应用 |
CN106755190A (zh) * | 2016-11-22 | 2017-05-31 | 大连大学 | 基于重组κ‑卡拉胶酶制备卡拉胶寡糖的方法 |
CN109929859A (zh) * | 2017-12-15 | 2019-06-25 | 中国科学院大连化学物理研究所 | 一种κ-卡拉胶酶编码基因及其制备与应用 |
CN109929859B (zh) * | 2017-12-15 | 2023-09-12 | 中科绿帅生物科技(广州)有限公司 | 一种κ-卡拉胶酶编码基因及其制备与应用 |
CN109762799A (zh) * | 2018-12-26 | 2019-05-17 | 青岛大学 | 一种κ-卡拉胶酶及其应用 |
CN110551716A (zh) * | 2019-09-10 | 2019-12-10 | 集美大学 | 基因Glu525的制备方法和用途 |
CN110551716B (zh) * | 2019-09-10 | 2021-05-07 | 集美大学 | 基因Glu525的制备方法和用途 |
CN111303310A (zh) * | 2020-03-12 | 2020-06-19 | 大连大学 | 一种海洋硫酸半乳六糖的制备方法和其在抗阿尔兹海默病药物制备中的应用 |
CN115725548A (zh) * | 2022-10-26 | 2023-03-03 | 集美大学 | 一种κ-卡拉胶酶Pcar16突变体及其制备方法 |
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