CN105918135A - Tissue culture system, tissue culture bottle conveying device thereof and LED (Light Emitting Diode) light incubator - Google Patents

Tissue culture system, tissue culture bottle conveying device thereof and LED (Light Emitting Diode) light incubator Download PDF

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Publication number
CN105918135A
CN105918135A CN201610472573.3A CN201610472573A CN105918135A CN 105918135 A CN105918135 A CN 105918135A CN 201610472573 A CN201610472573 A CN 201610472573A CN 105918135 A CN105918135 A CN 105918135A
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China
Prior art keywords
tissue culture
culture bottle
bottle
connecting shaft
self
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Granted
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CN201610472573.3A
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CN105918135B (en
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陆东利
陆慧丽
陆培玉
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Zhuhai Special Economic Zone Hung Yuan Garden Engineering Co Ltd
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Individual
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

The invention discloses a tissue culture system, a tissue culture bottle conveying device thereof and an LED (Light Emitting Diode) light incubator. The tissue culture bottle conveying device comprises a plurality of chains; all the chains are connected end to end to form a motion cycle in a vertical surface, are mutually parallel and are driven by the same driving device to synchronously move; a plurality of annular brackets are arranged between adjacent chains; each annular bracket comprises a support ring and a connecting shaft; a tissue culture bottle is placed in each support ring and is fixed in the corresponding support ring through a flange support on the circumference of a bottle body or a bottle cap; the connecting shaft of each annular bracket is connected with a link unit of the corresponding chain; each link unit comprises an outer link plate, an inner link plate, a roller, a sleeve and a self-lubricating bearing bush; each connecting shaft is inserted in the corresponding self-lubricating bearing bush; plane bearings and self-locking nuts are respectively arranged at two ends, penetrating out of the corresponding self-lubricating bearing, of each connecting shaft. According to the scheme, the tissue culture bottle conveying device is convenient for bearing and conveying of the tissue culture bottles; in addition, the tissue culture bottles automatically rotate for adjusting in the conveying process so as to facilitate light incubation.

Description

Group training system and tissue culture bottle conveyer device, LED lamplight incubator
The application is
Application No.: 201510299391.6
Filing date: on June 4th, 2015
Apply for entitled: group training system
The divisional application of patent application.
Technical field
The present invention relates to a kind of group for plants such as Herba Dendrobii and train system.
Background technology
Herba Dendrobii, is one of kind the most precious in China's the orchid family Dendrobium Sw.Because it has enhancing Immunity of organisms, antitumor, defying age and effectively alleviate the effect such as hepatic injury, version " middle Chinese in 2010 People republic pharmacopeia " in be individually classified as medical material simply, owing to artificially excessively excavating the destruction with ecological environment, In addition himself breeding potential is low, and dendrobium officinale resource is the most exhausted, is classified as two grades of emphasis by country and protects The rare or endangered species protected.Now, artificial culture plantation Herba Dendrobii has become and has met great market demand Main path.Herba Dendrobii system the orchid family Dendrobium Sw, seed is minimum, without need under endosperm, natural conditions with Some mycosymbiosis could be sprouted, and germination percentage only ten thousand in the case of nature/, and cultivar exists and degenerates Phenomenon.
In recent years, the group of Herba Dendrobii is trained and artificial culture skill by some scientific research institutions and the business unit of China Art has carried out substantial amounts of exploration and test, achieves some achievements, also makes artificial culture iron sheet stone in recent years Dry measure used in former times yield increased.But, the artificial culture of Herba Dendrobii yet suffers from that seeding propagation speed is slow, group training Seedling seedling exercising and the problem that transplanting survival rate is low, artificial growth product active constituent content is low, seriously constrain ferrum The industrialization of skin Herba Dendrobii, large-scale development.Additionally, the general field planting of Herba Dendrobii can be miscellaneous with growth Grass, and it is vulnerable to pest and disease damage.
The patent documentation of Publication No. CN102057867A discloses the tissue culture of a kind of seedlings of Dendrobium officinale Light regulation and control method, including obtaining powder seed by Dendrobium officinale fruit after being sterilized, powder seed is by planting Sub-germination medium is cultivated, and sprouts the process forming protocorm, it is characterised in that the tissue of seedlings of Dendrobium officinale In incubation, using LED (semiconductor light-emitting-diode) plant growth lighting system as light source, according to iron sheet The different phase of Herba Dendrobii tissue culture, implements different photo-irradiation treatment.By using different growth phases to implement Different photo-irradiation treatment, make seedlings of Dendrobium officinale can maximally utilise luminous energy and supply its photosynthesis, thus obtain Obtain maximum growth amount.
In the group training system of prior art, it is impossible to adjust red blue the light quality ratio of mixed light, light conveniently and efficiently According to intensity and periodicity of illumination.
Summary of the invention
In order to solve above-mentioned technical problem, it is an object of the invention to provide a kind of group training system, inside set LED Light incubator, use planar emission apparatus can conveniently adjust red blue mixed light light quality ratio, Intensity of illumination and periodicity of illumination.
To achieve the above object, present invention employs following technical scheme:
A kind of group training system, including totally enclosed group of training transfer room and artificial seedlings cultivating room, described group training inoculation Be disposed adjacent about room and artificial seedlings cultivating room, group training transfer room and artificial seedlings cultivating room entrance all with Airshower chamber Outlet connects, and arranges multiple inoculation platform and a conveyer belt, the outfan of conveyer belt in described group of training transfer room Stretching into artificial seedlings cultivating indoor, described artificial seedlings cultivating indoor are provided with room in seedlings cultivating, room in artificial seedlings cultivating room and seedlings cultivating Between only by several ventilation window ventilating airs, entered in seedlings cultivating by a sliding door from artificial seedlings cultivating room Room, in seedlings cultivating, indoor are provided with constant-temperature constant-humidity air-conditioner and multiple LED lamplight incubator;Described artificial seedlings cultivating room Being provided with several ventilation fans with ft connection on wall, the outside of artificial seedlings cultivating room is provided with steel framework, Sunshade net is laid at steel framework top, and sunshade net is furled by stay cord and launches, and steel framework is provided with confession The pulley of stay cord traction;Described LED lamplight incubator includes casing, is respectively provided with tissue culture bottle defeated in casing Send device and multiple planar emission apparatus;Tissue culture bottle conveyer device includes that multiple chain, each chain pass through chain Take turns in being arranged on casing and connect the motion cycle being formed in vertical plane, the motion cycle of multiple chains from beginning to end Parallel to each other and driven by same driving means and be synchronized with the movement, arrange between adjacent chain and multiple can place group The loop bracket of training bottle, so, multiple chains are formed in vertical plane the conveying knot of circulation conveying tissue culture bottle Structure, loop bracket includes filler ring and the connecting shaft of fixing connection, puts into tissue culture bottle in filler ring, and tissue culture bottle passes through Flange bearing on bottle or bottle cap circumference is fixed in filler ring, the connecting shaft of loop bracket and the chain link of chain Unit connects, and this link units includes outer plate, inner plate, roller, sleeve and self lubrication bearing, two Inner plate is fixedly connected to form inner link by sleeve, and bush outer sleeve sets roller, plugs self-lubricating axle in sleeve Set, self lubrication bearing passes sheathed two outer plates in two ends of sleeve, plugs connecting shaft in self lubrication bearing, Connecting shaft passes the two ends of self lubrication bearing and is all arranged with plane bearing and self-locking nut, self-locking nut be connected Male thread connects and is pressed in outside outer plate by plane bearing;Planar emission apparatus includes light guide plate, LED aluminum base Plate and radiating fin plate, LED aluminum base plate front is provided with multiple red LED lamp and multiple in the way of array distribution Blue LED lamp, the incidence surface of light guide plate presses close to the front of aluminium base, and radiating fin plate is close to the back of the body of aluminium base Face;In the conveying circulation that multiple chains that one planar emission apparatus is arranged on tissue culture bottle conveyer device are formed Portion, another planar emission apparatus is arranged on the conveying circulation that multiple chains of tissue culture bottle conveyer device are formed Outside, the conveying that the two planar emission apparatus is formed from multiple chains of tissue culture bottle conveyer device respectively follows The vertical plane inside and outside ring circulated this conveying carries out illumination.
As preferably, in described group of training transfer room, constant-temperature constant-humidity air-conditioner is installed.
As preferably, described inoculation platform includes cabinet, and before and after cabinet, both sides are transparent plastic sheet, cabinet Quartz burner is installed on the inwall of the left and right sides, cabinet inside be provided with temperature sensor, humidity sensor and Welding rifle, cabinet inwall top is provided with gas hole array, is provided with passage array bottom cabinet inwall.
As preferably, in described group of training transfer room, artificial seedlings cultivating room and seedlings cultivating room be all by aluminum alloy frame and Float glass process white glass builds.
As preferably, described tissue culture bottle includes that threaded bottle and bottle cap, bottle cap central authorities are provided with central canal, When there being culture medium in bottle, fill in aseptic Cotton Gossypii in central canal and by closing of sealing with wax.
As preferably, right inside and outside the conveying circulation that multiple chains of tissue culture bottle conveyer device are formed One vertical plane of this conveying circulation carries out two planar emission apparatus of illumination and is separately fixed at two mobile ends On seat, the two is moved base and is slidably connected by track level with casing, and box bottom sets up a screw mandrel, Screw mandrel is connected by plus thread and left-hand thread respectively two feed screw nuts, two feed screw nuts respectively with two Mobile base is fixing to be connected, and a motor or runner drive screw mandrel to rotate, thus drive two planar emission apparatus Synchronize close or remote respectively inside and outside the conveying circulation that multiple chains of tissue culture bottle conveyer device are formed From this conveying circulate by the vertical plane of illumination.
As preferably, red LED lamp and blue LED lamp in described LED aluminum base plate distribute alternately, and control Device controls each red LED lamp and blue LED lamp is individually lighted or extinguishes.As preferably, described red-light LED The power of lamp and blue LED lamp is 1W, and the red spectral band of red LED lamp is 450 ± 20nm, blue-ray LED The blue wave band of lamp is 660 ± 20nm.As preferably, described mobile base is at the radiating fin of planar emission apparatus Plate side frames is provided with and can promote the fan of circulation of air flow circulation in casing, described casing is provided with can enter The ventilation fan of air-flow exchange inside and outside row casing.
Due to the fact that and have employed above technical scheme, inside set LED lamplight incubator, use flat luminous Device can conveniently adjust light quality ratio, intensity of illumination and the periodicity of illumination of red blue mixed light.Logical Cross Airshower chamber entrance group training transfer room and artificial seedlings cultivating room ensure that group trains the cleanliness factor of room, artificial by arranging In seedlings cultivating room and seedlings cultivating, room ensure that the temperature and humidity that group training is indoor, arranges sunshade net and LED lamplight is cultivated Case, ensure that by natural light and artificial lighting the illumination of candidum tissue culturing seedling germination, growth and seedling exercising needs Want, so, the survival rate of Herba Dendrobii can not only be greatly improved, and saved the time cultivated, fall Low cultivation cost, improves economic benefit.
Accompanying drawing explanation
Fig. 1 is the structural representation of of the present invention group of training system;
Fig. 2 is the structural representation of LED lamplight incubator;
Fig. 3 is the partial structurtes schematic diagram of many chain conveying structures;
Fig. 4 is the cooperation schematic diagram of tissue culture bottle, loop bracket and link unit;
Fig. 5 is the moving structure schematic diagram of planar emission apparatus;
Fig. 6 is the aeration structure schematic diagram of planar emission apparatus.
Detailed description of the invention
Embodiments of the invention are described below in detail, and the example of described embodiment is shown in the drawings, wherein certainly Begin to same or similar label eventually represent same or similar element or there is the unit of same or like function Part.The embodiment described below with reference to accompanying drawing is exemplary, it is intended to be used for explaining the present invention, and not It is understood that as limitation of the present invention.
In describing the invention, it is to be understood that term " " center ", " longitudinally ", " laterally ", " length Degree ", " width ", " thickness ", " on ", D score, "front", "rear", "left", "right", " vertically ", " water Flat ", the orientation that indicates of " top ", " end " " interior ", " clockwise ", " counterclockwise " etc. or position relationship be based on Orientation shown in the drawings or position relationship, be for only for ease of the description present invention and simplify description rather than refer to Show or imply the device of indication or element must have specific orientation, with specific azimuth configuration and operation, Therefore it is not considered as limiting the invention.
Group training system as shown in Figure 1, including totally enclosed group of training transfer room 2 and artificial seedlings cultivating room 3, described Group training transfer room 2 and artificial seedlings cultivating room about 3 be disposed adjacent, group training transfer room 2 and artificial seedlings cultivating room 3 Entrance all outlets with Airshower chamber 1 be connected, in described group of training transfer room 2, multiple inoculation platform 21 and is set Bar conveyer belt 22, the outfan of conveyer belt 22 stretches in artificial seedlings cultivating room 3, sets in described artificial seedlings cultivating room 3 There is room 4 in seedlings cultivating, only by several ventilation window ventilating airs between room 4 in artificial seedlings cultivating room 3 and seedlings cultivating, Entered room 4 in seedlings cultivating by a sliding door from artificial seedlings cultivating room 3, in seedlings cultivating, in room 4, be provided with constant-temperature constant-humidity air-conditioner With multiple LED lamplight incubators 41;If being provided with and ft connection on the wall of described artificial seedlings cultivating room 3 Dry ventilation fan 31.The outside of artificial seedlings cultivating room 3 is provided with steel framework, and sunshade is laid at steel framework top Net, sunshade net is furled by stay cord and launches, and steel framework is provided with the pulley for stay cord traction.Institute In stating group training transfer room 2, constant-temperature constant-humidity air-conditioner is installed.Described inoculation platform 21 includes cabinet, before and after cabinet Both sides are transparent plastic sheet, and the left and right sides inwall of cabinet is provided with quartz burner, and cabinet inside is provided with Temperature sensor, humidity sensor and welding rifle, cabinet inwall top is provided with gas hole array, cabinet inwall Bottom is provided with passage array.In described group of training transfer room 2, artificial seedlings cultivating room 3 and seedlings cultivating, room 4 is all by aluminum Alloy framework and float glass process white glass build.Protected by Airshower chamber entrance group training transfer room and artificial seedlings cultivating room Demonstrate,prove the cleanliness factor of group training room, ensure that, by room in arranging artificial seedlings cultivating room and seedlings cultivating, the temperature that group training is indoor And humidity, sunshade net and LED lamplight incubator are set, ensure that iron sheet stone by natural light and artificial lighting Dry measure used in former times tissue cultured seedling germinates, grows and the illumination needs of seedling exercising, so, can not only be greatly improved Herba Dendrobii Survival rate, and saved the time cultivated, reduce cultivation cost, improve economic benefit.
As in figure 2 it is shown, described LED lamplight incubator includes casing 411, in casing 411, it is respectively provided with group training Bottle conveyer device and multiple planar emission apparatus 412;Tissue culture bottle conveyer device includes multiple chain 414, each In chain 414 is arranged on casing 411 by sprocket wheel and connect the motion cycle being formed in vertical plane from beginning to end, The motion cycle of multiple chains 414 is parallel to each other and is synchronized with the movement by the driving of same driving means, adjacent chain Arranging multiple loop bracket 415 that can place tissue culture bottle between 414, so, multiple chains 414 are formed for The structure for conveying of circulation conveying tissue culture bottle in vertical plane, as shown in Figure 3, Figure 4, loop bracket 415 includes The fixing filler ring 4151 connected and connecting shaft 4152, put into tissue culture bottle in filler ring 4151, tissue culture bottle passes through bottle Flange 622 supporting and fixing on body 61 or bottle cap 62 circumference in filler ring 4151, the company of loop bracket 415 Spindle 4152 is connected with the link units of chain 414, and this link units includes outer plate 4141, inner plate 4142, roller 4143, sleeve 4145 and self lubrication bearing 4147, two inner plates 4142 are by sleeve 4145 It is fixedly connected to form inner link, the outer sheathed roller 4143 of sleeve 4145, in sleeve 4145, plugs self-lubricating axle Set 4147, self lubrication bearing 4147 passes sheathed two outer plates 4141 in two ends of sleeve 4145, self-lubricating Plugging connecting shaft 4152 in axle sleeve 4147, the two ends that connecting shaft 4152 passes self lubrication bearing 4147 are the most sheathed Having plane bearing 4144 and self-locking nut 4146, self-locking nut 4146 is threadeded with connecting shaft 4152 and incites somebody to action Plane bearing 4144 is pressed in outside outer plate 4141;As it is shown in figure 5, planar emission apparatus 412 includes leading Tabula rasa 4121, LED aluminum base plate 4122 and radiating fin plate 4123, LED aluminum base plate 4122 front is divided with array The mode of cloth is provided with multiple red LED lamp and multiple blue LED lamp, and the incidence surface of light guide plate 4121 is pressed close to The front of aluminium base 4122, radiating fin plate 4123 is close to the back side of aluminium base 4122, uses light guide plate energy Enough light LED point light source bottom it sent penetrates with the form of area source after carrying out Mixed adjustment, illumination Uniform and reddish blue light mixes, low cost;As in figure 2 it is shown, a planar emission apparatus 412 is arranged The conveying circulation formed at multiple chains of tissue culture bottle conveyer device is internal, another planar emission apparatus 412 It is arranged on outside the conveying circulation that multiple chains of tissue culture bottle conveyer device are formed, the flat luminous dress of the two Put 412 defeated to this inside and outside the conveying circulation that multiple chains of tissue culture bottle conveyer device are formed respectively The vertical plane sending circulation carries out illumination.
In the present embodiment, as shown in Figure 4, described tissue culture bottle includes threaded bottle 61 and bottle cap 62, Bottle cap 62 central authorities are provided with central canal 621, when having culture medium in bottle 61, fill in aseptic in central canal 621 Cotton Gossypii 64 by 63 closings of sealing with wax.As it is shown in figure 5, formed from multiple chains of tissue culture bottle conveyer device Conveying circulation inside and outside a vertical plane that this conveying is circulated carry out two flat luminous dresses of illumination Putting 412 to be separately fixed on two mobile bases 413, the two moves base 413 and passes through with casing 411 Track level is slidably connected, and sets up a screw mandrel 416 bottom casing 411, screw mandrel 416 respectively by plus thread and Left-hand thread connects two feed screw nuts 417, two feed screw nuts 417 respectively with two mobile bases 413 Fixing connection, a motor 418 or runner drive screw mandrel 416 to rotate, thus drive two planar emission apparatus 412 respectively from multiple chains of tissue culture bottle conveyer device formed conveying circulation inside and outside synchronize close Or away from this conveying circulate by the vertical plane of illumination.So by adjusting the spacing of two planar emission apparatus, Just can adjust the planar emission apparatus intensity of illumination to group training face, the equipment suitability is higher, low cost.As Employing motor controls, and arranges corresponding light sensor in casing and just can realize the automatic of intensity of illumination Control.The power of described red LED lamp and blue LED lamp is 1W, and the red spectral band of red LED lamp is 450 ± 20nm, the blue wave band of blue LED lamp is 660 ± 20nm.Red in described LED aluminum base plate 4122 Light LED and blue LED lamp distribute alternately with spacing 2cm × 2cm, and controller controls each red LED lamp Individually light with blue LED lamp or extinguish, so by controlling lighting of red LED lamp and blue LED lamp Quantity and distribution just can adjust the light quality ratio of planar emission apparatus easily.Described mobile base 413 exists Radiating fin plate 4123 side frames of planar emission apparatus 412 is provided with and can promote circulation of air flow stream in casing 411 The fan 419 turned, described casing 411 is provided with and can carry out the ventilation fan of air-flow exchange inside and outside casing 411 420.So, LED can not only be helped to dispel the heat, and these heats can be utilized to maintain LED lamplight Temperature in incubator, when LED lamplight cultivate the temperature inside the box higher time, it is also possible to heat radiation to room in seedlings cultivating etc..
Although above it has been shown and described that embodiments of the invention, it is to be understood that above-described embodiment Being exemplary, it is impossible to be interpreted as limitation of the present invention, those of ordinary skill in the art is without departing from this Above-described embodiment can be changed within the scope of the invention in the case of the principle of invention and objective, repair Change, replace and modification.

Claims (3)

1. tissue culture bottle conveyer device, it is characterised in that include multiple chain (414), each chain (414) It is arranged in casing (411) by sprocket wheel and connects the motion cycle being formed in vertical plane from beginning to end, The motion cycle of multiple chains (414) is parallel to each other and is synchronized with the movement by the driving of same driving means, Multiple loop bracket (415) that can place tissue culture bottle is set between adjacent chain (414), so, Multiple chains (414) are formed in vertical plane the structure for conveying of circulation conveying tissue culture bottle, annular torr Frame (415) includes filler ring (4151) and connecting shaft (4152), the filler ring (4151) of fixing connection Inside putting into tissue culture bottle, tissue culture bottle is by the flange (622) on bottle (61) or bottle cap (62) circumference Supporting and fixing in filler ring (4151), the connecting shaft (4152) of loop bracket (415) and chain (414) link units connects, this link units include outer plate (4141), inner plate (4142), Roller (4143), sleeve (4145) and self lubrication bearing (4147), two inner plates (4142) It is fixedly connected to form inner link by sleeve (4145), sleeve (4145) the most sheathed roller (4143), Plugging self lubrication bearing (4147) in sleeve (4145), self lubrication bearing (4147) passes sleeve (4145) sheathed two outer plates (4141) in two ends, the company of plugging in self lubrication bearing (4147) Spindle (4152), connecting shaft (4152) passes the two ends of self lubrication bearing (4147) and is all arranged with Plane bearing (4144) and self-locking nut (4146), self-locking nut (4146) and connecting shaft (4152) Threaded and plane bearing (4144) is pressed in outer plate (4141) outside;One flat luminous Device (412) is arranged on the conveying circulation inside that multiple chains of tissue culture bottle conveyer device are formed, Another planar emission apparatus (412) is arranged on what multiple chains of tissue culture bottle conveyer device were formed Outside conveying circulation, the two planar emission apparatus (412) is many from tissue culture bottle conveyer device respectively The vertical plane inside and outside the conveying circulation that individual chain is formed circulated this conveying carries out light According to;Described tissue culture bottle includes threaded bottle (61) and bottle cap (62), in bottle cap (62) Centre is provided with central canal (621), when having culture medium in bottle (61), and plug in central canal (621) Enter aseptic Cotton Gossypii (64) and closed by seal with wax (63).
2. a LED lamplight incubator, it is characterised in that include that tissue culture bottle as claimed in claim 1 carries Device.
3. a group training system, it is characterised in that include that a kind of LED lamplight as claimed in claim 2 is cultivated Case.
CN201610472573.3A 2015-06-04 2015-06-04 Tissue culture system and its tissue culture bottle conveying device, LED light light incubator Expired - Fee Related CN105918135B (en)

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CN201610472573.3A CN105918135B (en) 2015-06-04 2015-06-04 Tissue culture system and its tissue culture bottle conveying device, LED light light incubator
CN201510299391.6A CN104855291B (en) 2015-06-04 2015-06-04 Tissue culture system

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CN201610472548.5A Expired - Fee Related CN105993957B (en) 2015-06-04 2015-06-04 Tissue culture system and its led light incubator
CN201510299391.6A Expired - Fee Related CN104855291B (en) 2015-06-04 2015-06-04 Tissue culture system
CN201610442519.4A Active CN106106146B (en) 2015-06-04 2015-06-04 Tissue culture system and its planar emission apparatus, LED light light incubator
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CN201510299391.6A Expired - Fee Related CN104855291B (en) 2015-06-04 2015-06-04 Tissue culture system
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