CN105900886A - Method for indoors breeding oxyeleotris lineolatus fry by means of organism bait - Google Patents

Method for indoors breeding oxyeleotris lineolatus fry by means of organism bait Download PDF

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Publication number
CN105900886A
CN105900886A CN201610335223.2A CN201610335223A CN105900886A CN 105900886 A CN105900886 A CN 105900886A CN 201610335223 A CN201610335223 A CN 201610335223A CN 105900886 A CN105900886 A CN 105900886A
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fry
feeding
bait
something
density
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CN105900886B (en
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李飞
郭建林
刘士力
顾志敏
张宇飞
李枫
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Zhejiang Institute of Freshwater Fisheries
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Zhejiang Institute of Freshwater Fisheries
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses a method for indoors breeding oxyeleotris lineolatus fry by means of organism bait. The method comprises the following steps of parental selection and strengthening breeding, natural spawning and incubation management and fry breeding. According to the method, breeding of the oxyeleotris lineolatus fry is carried out indoors, the whole breeding process is visible and controllable, and breeding conditions such as the water temperature, the water quality and dissolved oxygen all tend to be stable; according to the requirements of the oxyeleotris lineolatus fry at different development stages on bait, the organism bait is utilized for accurately breeding the fry in a cement pool; on one hand, the requirements of the fry on bait varieties are guaranteed; on the other hand, due to small water body breeding, the fed bait is easy to ingest; therefore, the utilization efficiency of the bait is greatly improved, and not only can the breeding survival rate of the fry be increased, but also the fry breeding cost can be reduced.

Description

A kind of method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry
Technical field
The invention belongs to aquatic animal raising technology field, be specifically related to one and utilize biological feed indoor to breed strain line point The method of sleeper fry.
Background technology
Oxyeleotris lineolatus (Oxyeleotris lineolatus), English name sleepy cod, classification is under the jurisdiction of Perciformes (Perciformes), goby suborder (Gobioidei), Eleotridae (Eleotridae), Oxyeleotris (Oxyeleotris). It is referred to as sleeping cod in Australia;It is referred to as Australia bamboo shell fish in Hong Kong and Macao of China, In Guangdong Province.It is big that Oxyeleotris lineolatus originates in Australia Leah, suitable water temperature range is 15-35 DEG C, and less than 15 DEG C energies substantially weaken, and semilethal low temperature lower bound is 8.5 DEG C, generally It is distributed in pasture and water and flat water, eating small fish, shrimp etc. sluggishly.This meat quality of fish is delicate, and delicious flavour, deeply by state's internal diabetes The person of expense welcomes.China in last century the nineties start to introduce this fish, and carry out in pond raising and train, breed and tentatively obtaining into Merit.But because of this kind, to introduce China's time the longest, and newly hatched larvae individuality is less, only 2.875mm, and prelarva includes mixing battalion Supporting alevin stage, the time is short, only about 5d, belongs to the Fish relatively early setting up exogenous nutrition feeding mechanism, therefore seed rearing skill It is the most immature that art compares other Common Fishs.It has been observed that Oxyeleotris lineolatus from endogenous nutrition to exogenous nutrition mistake Spend interim, although the oily ball in yolk sac can be relied on to provide energy, but the most limited, and hatching second day some individuality can open Mouth is ingested, and hatches the of short duration 3 day mixotrophism phase that just enters for the 3rd day, hatches and transfer pure exogenous nutrition on the 5th day.Natural Water body or man-made pond(pool), all cannot provide the open-mouthed bait that prelarva is fully sufficient, thus occur that prelarva dies young in a large number; Secondly as prelarva individuality is little, keeping away enemy's ability, easily become the object of ingesting of Copepods in water body, cladocera, these are all Considerably increase the seed rearing difficulty of this fish, reduce its cultivating survival rate.
At present, the seed rearing majority of Oxyeleotris lineolatus still takes traditional Tu Chi to cultivate pattern.Owing to being outdoor training Supporting, this seedling raising manners is highly prone to the impact of the environmental factors such as weather, water temperature, adds and cannot provide for the fry in each stage Sufficient agreeable to the taste bait, causes the cultivating survival rate of fry to be only 15-20%.It is contemplated that according to the biology of Oxyeleotris lineolatus Learn characteristic, in its mating period, in greenhouse, in cement pit, carry out artificial propagation and seed rearing, utilize freshwater microalgae and fresh water The biological feeds such as wheel animalcule precisely provide the bait in Oxyeleotris lineolatus fry growth each stage, thus are greatly improved seed rearing and survive Rate.
Summary of the invention
For problems of the prior art, present invention aim at providing one to utilize biological feed indoor to breed line The method of stricture of vagina Oxyeleotris marmoratus fry.
The present invention is realized by the following technical programs:
A kind of described method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry, it is characterised in that by following steps group Become:
1) Juvenile stage and reinforced cultivating
Northern Zhejiang area in mid-June select that vigor is good, squamation complete, the female milter of whose body weight about 400-500g is as parent This, be that 1:1 puts in a suitable place to breed in cement pit according to sex ration, and breeding density is 1 tail/m2, the depth of water is 60cm, and in cement pit, water is warp The clarification pond water of 120 mesh silk cover filterings, water temperature is 25-28 DEG C, and Roots blower and gas exhausting stone are uninterruptedly inflated;Parent The fish nurturing period, the throw something and feed fresh chilled flesh of fish and Macrobrachium nipponensis(de Haan) are as bait, and the mode of throwing something and feeding of the chilled flesh of fish is for be cut into meat by the flesh of fish Then fourth throws something and feeds, and the mode of throwing something and feeding of Macrobrachium nipponensis(de Haan) is thrown something and fed to parent's reinforced cultivating pond for live body after light salt brine is sterilized, and total feeding volume is about For the 5-8% of parent's body weight, often cement pit being carried out water injection once in 2-3 days, displacement is 1/3, to stimulate parent fish gonad simultaneously Grow;
2) natural spawning and hatching management
After entering mating period, female milter carries out nature pairing in cement pit, mutually chases, lays eggs, and the ovum produced adheres to On cement pit pool wall, laying eggs after end, milter is kept alone by germ cell, frequently swings pectoral fin and tail fin, promotes by germ cell Current produce fine motion, thus ensure germ cell periphery have abundance dissolved oxygen;When cement pit has 1-2 sheet germ cell, can be Let alone in the case of protecting ovum milter as far as possible, other female milters are pulled out put to other cement pits proceed cultivate, make its from So lay eggs, due to raun can many batches lay eggs, therefore raun in puerperal is pulled out in the lump, thus improves the utilization rate of parent;
During incubating oosperm, keep uninterrupted inflation, protect ovum milter to every day every tail simultaneously and throw something and feed 4-5 ice cube fresh fish meat cubelets, To ensure its nutrition and muscle power;Under conditions of water temperature 25-28 DEG C, through 2.5-3d, prelarva progressively hatches, and treats that germ cell is all incubated After dissolving film, go to new parent culture pond by protecting ovum milter;Utilize this kind of hatching pattern, owing to there being milter to protect ovum, can be significantly Improving the incubation rate of germ cell, incubation rate is up to more than 95%;
3) fry rearing
After incubation of membrane 2d, the yolk sac of prelarva is absorbed complete substantially, and beginning level is travelling and looks for food, and can carry out fry training Educate;Cultivate and carry out in former spawning pond, the damage that fry is produced by manual operation can be reduced, from fry opening to total length length to 2- 3cm, uses microalgae and freshwater rotifer, fairy shrimp, cladocera and Copepods, big artemia and fish meat emulsion or flesh of fish fourth as bait successively Cultivating, concrete cultivation and management method are as follows:
First stage: 3d-10d, owing to the opening bore of prelarva only has 200-300 μm, selects microalgae and freshwater rotifer as fish The open-mouthed bait of Seedling, this is also the bait that this fry rearing is the most key, and microalgae density of throwing something and feeding is 0.2-0.5*106Ind/ml, Freshwater rotifer density of throwing something and feeding is 8-10ind/ml, in this stage, throws something and feeds 2-3 time every day, remains microalgae and freshwater rotifer Throw something and feed density;The open-mouthed bait of Oxyeleotris lineolatus fry, the cultivating survival rate in this stage can be solved well by this kind of method Can reach more than 95%;
Second stage: 11d-20d, the open-mouthed bait problem of this stage major part fry solves, can gradually decrease freshwater rotifer Throw something and feed, start simultaneously at fairy shrimp of throwing something and feeding;After this stage terminates, fry can grow to about 1.0cm;
Phase III: 20d-30d, this stage starts to throw something and feed the cladocera after 80 mesh samples are sieved through filter fished for from pond and oar foot Class, bait feeding density is 3-5ind/ml, throws something and feeds 2-3 time every day, makes bait remain at this scope;This stage terminates After, fry can grow to about 2.0cm;
Fourth stage: 30d-50d, fry carries out point supporting, an original pond divides and support to two ponds by this stage, after point supporting Density is about 80 tails/m2;Bait feeding aspect, reduces cladocera and the feeding volume of Copepods, and starts manual chopping of throwing something and feeding Chilled flesh of fish meat paste, < 1.0mm with finger flicks the water surface before throwing something and feeding every time, tames, then gradually thrown by meat paste its particle diameter Fry before being concentrated to body;Along with gradually growing up of fry, the particle diameter of fish meat emulsion becomes larger to 2-3mm, and can start Throw something and feed frost big artemia as bait, feeding volume is had enough gradually to scatter with fry and is as the criterion, and throws something and feeds every day 2 times.
A kind of described method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry, it is characterised in that in step 1) A length of 6m of described cement pit, a width of 2m;The specification of Macrobrachium nipponensis(de Haan) be 1800-2000 tail/kilogram, the specification of meat cubelets is that length, width and height are each For 0.5cm, the salinity of described light salt brine is 2-3%, and the chilled flesh of fish thrown something and fed and the weight ratio of Macrobrachium nipponensis(de Haan) are 3:1.
A kind of described method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry, it is characterised in that in step 3) Microalgae be one or more in chlorella, scenedesmus or chlamydomonas.
A kind of described method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry, it is characterised in that in step 3) Freshwater rotifer is one or more in Brachionus calyciflorus, brachionus plicatilis and Branchionus urceus.
A kind of described method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry, it is characterised in that step 3) The feeding volume of two-stage freshwater rotifer, for being 5-8ind/ml in 11d density of throwing something and feeding, then gradually decreases, and 12-14d throws something and feeds Density be 3-6ind/ml, 15-19d density of throwing something and feeding be 1-3ind/ml, stop throwing something and feeding freshwater rotifer to 20d;Fairy shrimp exists Density of throwing something and feeding during 11d is 1ind/ml, is then gradually increased, and 12-14d density of throwing something and feeding every day is 2-3ind/ml, 15- 19d density of throwing something and feeding every day is 3-4ind/ml, is 5ind/ml to the density of throwing something and feeding of fairy shrimp during 20d.
A kind of described method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry, it is characterised in that step 3) The density of throwing something and feeding of four stage cladoceras and Copepods is 0.5ind/ml.
A kind of described method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry, it is characterised in that step 3) Four stages, at an indoor water mud sump 100w electric filament lamp installed above, were used for attracting midge to laying eggs in cement pit and hatching generation Chironomus larvas is as the bait of fry.Through the cultivation of fourth stage, fry, up to about 3cm, i.e. may be sold or puts in a suitable place to breed Starting large size fingerling to cultivate and adult fish culture to pond, fry rearing survival rate is up to more than 85%.
The present invention, by carrying out breeding of Oxyeleotris lineolatus fry in indoor, makes to breed whole process more visible controlled, water temperature, Water quality, dissolved oxygen etc. are bred condition and are all tended towards stability;According to the bait demand of Oxyeleotris lineolatus fry different developmental phases, utilize Fry is precisely cultivated in cement pit by biological feed, on the one hand ensure that the fry demand to food species, the opposing party Face, because being that small size pond is cultivated so that the bait thrown something and fed is prone to ingest, thus substantially increases the utilization ratio of bait, finally Not only can improve the cultivating survival rate of fry, also can save seedling cost.
Accompanying drawing explanation
Fig. 1 is different days Oxyeleotris lineolatus fish total length change curve in cultivating process in embodiment 1;
Fig. 2 is that male parent protects ovum figure;
Fig. 3 is the fry figure of firm membrane;
Fig. 4 is successfully the fry figure of opening;
Fig. 5 is to cultivate the Oxyeleotris lineolatus fry figure to 2cm.
Detailed description of the invention
By specific examples below, the invention will be further described.
Embodiment 1
With one of them 12m in 20122As a example by cement pit, laid eggs in this cement pit July 2 a piece of after, ovum amount is less, utilize hero Fish carries out protecting egg hatching, and by other parent transfer to other cement pits.Under conditions of water temperature 29 DEG C, July 5, germ cell was opened Beginning incubation of membrane, whole membrane, also removed this pond proceed by fry rearing with protecting ovum milter July 6.According to this patent In breeding method, successively with microalgae, freshwater rotifer, fairy shrimp, cladocera, Copepods, fish meat emulsion (fourth) and freeze big artemia Cultivating for bait, to August 16, successful incubation went out Oxyeleotris lineolatus fry 9100 tail of about 3cm, and cultivating survival rate is about It is 70%.
Wherein, in cultivating process, different days Oxyeleotris lineolatus fish total length change curve is as shown in Figure 1;Male parent protects Ovum is as shown in Figure 2;Just the fry of membrane is as shown in Figure 3;The fry of success opening is as shown in Figure 4;Cultivate the strain line point pool to 2cm Channa argus Seedling is as shown in Figure 5.
Embodiment 2
With one of them 12m in 20132As a example by cement pit, use same method to carry out breeding of Oxyeleotris lineolatus, cultivate 1cm Oxyeleotris lineolatus fry about 20,000 tail, cultivating survival rate about 95%, cultivate to Oxyeleotris lineolatus fry during 3cm about 1.8 ten thousand tail, Cultivating survival rate about 85%.

Claims (10)

1. one kind utilizes the method that Oxyeleotris lineolatus fry is bred in biological feed indoor, it is characterised in that comprise the steps of:
1) Juvenile stage and reinforced cultivating
Northern Zhejiang area in mid-June select that vigor is good, squamation complete, the female milter of whose body weight about 400-500g is as parent This, be that 1:1 puts in a suitable place to breed in cement pit according to sex ration, and breeding density is 1 tail/m2, the depth of water is 60cm, and in cement pit, water is warp The clarification pond water of 120 mesh silk cover filterings, water temperature is 25-28 DEG C, and Roots blower and gas exhausting stone are uninterruptedly inflated;Parent The fish nurturing period, the throw something and feed fresh chilled flesh of fish and Macrobrachium nipponensis(de Haan) are as bait, and the mode of throwing something and feeding of the chilled flesh of fish is for be cut into meat by the flesh of fish Then fourth throws something and feeds, and the mode of throwing something and feeding of Macrobrachium nipponensis(de Haan) is thrown something and fed to parent's reinforced cultivating pond for live body after light salt brine is sterilized, and total feeding volume is about For the 5-8% of parent's body weight, often cement pit being carried out water injection once in 2-3 days, displacement is 1/3, to stimulate parent fish gonad simultaneously Grow;
2) natural spawning and hatching management
After entering mating period, female milter carries out nature pairing in cement pit, mutually chases, lays eggs, and the ovum produced adheres to On cement pit pool wall, laying eggs after end, milter is kept alone by germ cell, frequently swings pectoral fin and tail fin, promotes by germ cell Current produce fine motion, thus ensure germ cell periphery have abundance dissolved oxygen.
2., when cement pit has 1-2 sheet germ cell, in the case of letting alone and protecting ovum milter, other female milters are pulled out and puts to it His cement pit proceeds cultivate and make its natural spawning;
During incubating oosperm, keep uninterrupted inflation, protect ovum milter to every day every tail simultaneously and throw something and feed 4-5 ice cube fresh fish meat cubelets, To ensure its nutrition and muscle power;Under conditions of water temperature 25-28 DEG C, through 2.5-3d, prelarva progressively hatches, and treats that germ cell is all incubated After dissolving film, go to new parent culture pond by protecting ovum milter;
3) fry rearing
After incubation of membrane 2d, the yolk sac of prelarva is absorbed complete substantially, and beginning level is travelling and looks for food, and can carry out fry training Educate.
3. cultivate and carry out in former spawning pond, from fry opening to total length length to 2-3cm, successively with microalgae and freshwater rotifer, rich Year worm, cladocera and Copepods, big artemia and fish meat emulsion or flesh of fish fourth are cultivated as bait, concrete cultivate and management method As follows:
First stage: 3d-10d, owing to the opening bore of prelarva only has 200-300 μm, selects microalgae and freshwater rotifer as fish The open-mouthed bait of Seedling, microalgae density of throwing something and feeding is 0.2-0.5*106Ind/ml, freshwater rotifer density of throwing something and feeding is 8-10ind/ml, often It is thrown something and fed 2-3 time, remains the density of throwing something and feeding of microalgae and freshwater rotifer;
Second stage: 11d-20d, the open-mouthed bait problem of this stage major part fry solves, can gradually decrease freshwater rotifer Throw something and feed, start simultaneously at fairy shrimp of throwing something and feeding;
Phase III: 20d-30d, this stage starts to throw something and feed the cladocera after 80 mesh samples are sieved through filter fished for from pond and oar foot Class, bait feeding density is 3-5ind/ml, throws something and feeds 2-3 time every day, makes bait remain at this scope;
Fourth stage: 30d-50d, fry carries out point supporting, an original pond divides and support to two ponds by this stage, after point supporting Density is about 80 tails/m2;Bait feeding aspect, reduces cladocera and the feeding volume of Copepods, and starts manual chopping of throwing something and feeding Chilled flesh of fish meat paste, < 1.0mm with finger flicks the water surface before throwing something and feeding every time, tames, then gradually thrown by meat paste its particle diameter Fry before being concentrated to body.
4. gradually growing up along with fry, the particle diameter of fish meat emulsion becomes larger to 2-3mm, and can start the big artemia freezed of throwing something and feeding As bait, feeding volume is had enough gradually to scatter with fry and is as the criterion, and throws something and feeds every day 2 times.
A kind of method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry the most according to claim 1, its feature It is a length of 6m of the cement pit described in step 1), a width of 2m;The specification of Macrobrachium nipponensis(de Haan) be 1800-2000 tail/kilogram, the rule of meat cubelets Lattice be length, width and height respectively for 0.5cm, the salinity of described light salt brine is 2-3%, and the chilled flesh of fish thrown something and fed and the weight ratio of Macrobrachium nipponensis(de Haan) are 3:1.
A kind of method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry the most according to claim 1, its feature The microalgae being in step 3) is one or more in chlorella, scenedesmus or chlamydomonas.
A kind of method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry the most according to claim 1, its feature Be the one during freshwater rotifer is Brachionus calyciflorus, brachionus plicatilis and Branchionus urceus in step 3) or one with On.
A kind of method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry the most according to claim 1, its feature It is that the feeding volume of step 3) second stage freshwater rotifer, for being 5-8ind/ml in 11d density of throwing something and feeding, then gradually decreases, 12-14d density of throwing something and feeding is 3-6ind/ml, and 15-19d density of throwing something and feeding is 1-3ind/ml, stops, to 20d, fresh water wheel of throwing something and feeding Worm;Fairy shrimp density of throwing something and feeding when 11d is 1ind/ml, is then gradually increased, and 12-14d density of throwing something and feeding every day is 2- 3ind/ml, 15-19d density of throwing something and feeding every day is 3-4ind/ml, is 5ind/ml to the density of throwing something and feeding of fairy shrimp during 20d.
A kind of method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry the most according to claim 1, its feature The density of throwing something and feeding being step 3) fourth stage cladocera and Copepods is 0.5ind/ml.
A kind of method utilizing biological feed indoor to breed Oxyeleotris lineolatus fry the most according to claim 1, its feature It is that step 3) fourth stage, at an indoor water mud sump 100w electric filament lamp installed above, is used for attracting midge to produce to cement pit Ovum and hatching produce the chironomus larvas bait as fry.
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Publication number Priority date Publication date Assignee Title
CN112772470A (en) * 2020-12-31 2021-05-11 中国水产科学研究院黑龙江水产研究所 Artificial cultivation method of juvenile glyptosternum maculatum

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