CN105816635A - Pharmaceutical composition for treating cat tetanus and preparing method thereof - Google Patents

Pharmaceutical composition for treating cat tetanus and preparing method thereof Download PDF

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CN105816635A
CN105816635A CN201610384924.5A CN201610384924A CN105816635A CN 105816635 A CN105816635 A CN 105816635A CN 201610384924 A CN201610384924 A CN 201610384924A CN 105816635 A CN105816635 A CN 105816635A
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weight portion
radix
pharmaceutical composition
folium
powder
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傅建功
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Yantai Minwang Electromechanical Technology Co Ltd
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Yantai Minwang Electromechanical Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/11—Pteridophyta or Filicophyta (ferns)
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
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Abstract

The invention discloses a pharmaceutical composition for treating cat tetanus and a preparing method thereof .The pharmaceutical composition comprises common clubmoss herbs, leaves of Japanese glorybower, radix ampelopsis, cuttlefish bone, angelica sinensis, paper mulberry leaves, chestnut endocarp, cissus hexangularis, flower of Japanese rose, tangerine pith, polygonum chinense, diaphragma juglandis, radix clematidis, henry sarmentose fig receptacle, silktree albizzia bark, polygonum suffultum, large-leaved gentian, roots of apricotleaf ladybell, paniculate swallowwort roots, callicarpa bodinieri and liquorice roots .The pharmaceutical composition has the advantages that in the cat tetanus disease treatment aspect, the pharmaceutical composition is high in curing rate and short in treatment course, the immunity of a cat can be further enhanced, the death rate is decreased, and cost price is low.

Description

One is used for treating tetanic pharmaceutical composition of cat and preparation method thereof
Technical field
The present invention relates to technical field of medicine, be used for treating tetanic pharmaceutical composition of cat and preparation method thereof particularly to one.
Background technology
Cat tetanus is to be invaded wound growth and breeding by clostridium tetani and secrete clostridium tetani toxin and the acute infectious disease of Zoonosis that causes, is characterized with central nervous system's elevation of irritability, muscle and ankylosis.
Clostridium tetani is Gram-positive escherichia coli, tool week flagellum, without pod membrane, brood cell can be formed.Clostridium tetani can produce two kinds of extracellular toxins, and one is sphaceltoxin, mainly acts on nervous system, and another kind is hemotoxin.
After the brood cell of clostridium tetani enters wound and the deep wounds of cat, because of the growth and breeding of the anaerobic environment in wound deep, beneficially clostridium tetani, so breeding in a large number, producing extracellular toxin, causing cat to fall ill.In the case of nature, cat, dog all can infect, and other many animals and people also can infect.
Ill cat muscle and joint are spasmodic rigdity, and mannequin's steps state is stiff, tail are lifted, in typical opisthotonus posture.Instant embrane is benumbed and is highlighted, and pupil increases, and chews dysphagia, sialorrhea.To touching, light and sound reflexive strengthen.
The tetanic principle of western medical treatment cat mainly guarantee respiratory tract, blood vessel, urethra unobstructed.Outer wound response is once occurred to clear up the foreign body removed in wound and slough, pus in time, with the hydrogen peroxide irrigation and disinfection of 3%；To ill cat intensive care, reduce and stimulate.Sick cat should be placed in relatively quiet dark room, and note feeding, it is impossible to enter trencherman and then answer fluid infusion；Injection tetanus antitoxin.Tetanus antiserum be treatment primary disease specific medicament, can intravenous injection 100 1000 units/kg body weight, be used in conjunction 2-3 time.
Chinese veterinarian thinks, tetanus refers to first there is broken wound, and the heresy of pathogenic wind toxic is invaded by wound and causes a kind of disease of infantile convulsion.Tetanus name of disease head sees Song dynasty Holy Benevolent Prescriptions, cloud: " orthotonus, locked mouth can not open, and extremity tremble, diaphysis pain, appearance fish sticking its mouth out of the water tiltedly, this all damages in part in ailment said due to cold or exposure, therefore named tetanus." heresy of pathogenic wind toxic is widely present, does not occur only at the broken wound of skin and flesh, sterilize strict in operation, or neonatal umbilical cord is polluted, or produce and miscarry mishandling, or decubital ulcer contamination, it is possible to cause tetanus.Person caused by wound, claims gold wound convulsion；Send out survivor puerperal, claim postpartum convulsion；Neonate breaks person caused by umbilicus, claims infantum tetanus to take the photograph mouth.
The cause of disease is the broken wound of skin and flesh, experiences again the heresy of pathogenic wind toxic.The heresy of pathogenic wind toxic is attacked in people in place of taking advantage of the broken wound of skin and flesh, wind being YANG-pathogen, benefaction and number becomes, exogenous wind priming liver-wind stirring up internally, with the passing of time pathogenic wind toxic people invades, heat-transformation fire-transformation, makes zang-fu disharmony, qi-blood disharmony, deficiency of YIN affecting YANG, even dissociation of YIN from YANG and dead.Pathogenic wind toxic, at the slight dysphagia of table, gnathospasma, all over the body contracture, is twitched relatively light, and spasm period is short, and the intermission is longer.Pathogenic wind toxic enters inner tic of the limbs, opisthotonus, and high heat shiver with cold, whole-body muscle spasm, the intermission is short, rapid breathing, accumulation and obstruction of sputum, constipation, urinary obstruction；It is numb that the deficiency of YIN loses foster extremity, joint stuffiness.
Western modern medicine treatment cat tetanus, effect is undesirable, and the course for the treatment of is long, and PD is rapid, wayward.Chinese medicine not only has the advantage of Western medicine, and treats cat tetanus cure rate height, by determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs, can strengthen cat immunity, reduce mortality rate.Therefore, Chinese medicine, in terms for the treatment of cat tetanus disease, has Western medicine and does not has the advantage that, the most increasingly favored by people.
Summary of the invention
The technical problem to be solved is: providing one to be used for treating tetanic pharmaceutical composition of cat and preparation method thereof, treatment cat tetanus cure rate is high, by determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs, can strengthen cat immunity, and cost price is cheap.Medicine, in terms for the treatment of cat tetanus disease, has Western medicine and does not has the advantage that.
In order to realize foregoing invention purpose, the invention provides a kind of for treating the tetanic pharmaceutical composition of cat, described medicine includes following raw medicinal material: Herba Lycopodii, Folium paulowniae, Radix Ampelopsis, Endoconcha Sepiae, Radix Angelicae Sinensis, Folium Broussonetiae, chestnut, Radix Cissi hexangularis, white residual flower, Vascular aurantii, Herb Polygoni Chinensis, Partition in Fructus Juglandis, Radix Clematidis, Receptaculum fici Henryi, Cortex Albiziae, RHIZOMA POLYGONI SUFFULTI, Radix Gentianae Macrophyllae, shepherd's purse, Radix Cynanchi Paniculati, Folium Callicarpae Formosanae and Radix Glycyrrhizae.
nullDescribed pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 12-24 weight portion、Folium paulowniae 15-35 weight portion、Radix Ampelopsis 14-32 weight portion、Endoconcha Sepiae 13-27 weight portion、Radix Angelicae Sinensis 15-40 weight portion、Folium Broussonetiae 16-28 weight portion、Chestnut 12-32 weight portion、Radix Cissi hexangularis 15-25 weight portion、White residual flower 14-42 weight portion、Vascular aurantii 12-24 weight portion、Herb Polygoni Chinensis 13-31 weight portion、Partition in Fructus Juglandis 16-33 weight portion、Radix Clematidis 12-26 weight portion、Receptaculum fici Henryi 10-20 weight portion、Cortex Albiziae 14-32 weight portion、RHIZOMA POLYGONI SUFFULTI 11-21 weight portion、Radix Gentianae Macrophyllae 14-36 weight portion、Shepherd's purse 15-35 weight portion、Radix Cynanchi Paniculati 16-32 weight portion、Folium Callicarpae Formosanae 12-24 weight portion and Radix Glycyrrhizae 15-35 weight portion.
nullDescribed pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 14-23 weight portion、Folium paulowniae 15-35 weight portion、Radix Ampelopsis 15-30 weight portion、Endoconcha Sepiae 15-25 weight portion、Radix Angelicae Sinensis 15-40 weight portion、Folium Broussonetiae 18-26 weight portion、Chestnut 12-30 weight portion、Radix Cissi hexangularis 15-25 weight portion、White residual flower 15-40 weight portion、Vascular aurantii 12-24 weight portion、Herb Polygoni Chinensis 13-30 weight portion、Partition in Fructus Juglandis 16-32 weight portion、Radix Clematidis 12-25 weight portion、Receptaculum fici Henryi 10-20 weight portion、Cortex Albiziae 15-30 weight portion、RHIZOMA POLYGONI SUFFULTI 12-20 weight portion、Radix Gentianae Macrophyllae 15-35 weight portion、Shepherd's purse 15-34 weight portion、Radix Cynanchi Paniculati 15-32 weight portion、Folium Callicarpae Formosanae 14-22 weight portion and Radix Glycyrrhizae 15-35 weight portion.
Described pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 22 weight portion, Folium paulowniae 32 weight portion, Radix Ampelopsis 25 weight portion, Endoconcha Sepiae 18 weight portion, Radix Angelicae Sinensis 40 weight portion, Folium Broussonetiae 25 weight portion, chestnut 16 weight portion, Radix Cissi hexangularis 24 weight portion, white residual flower 40 weight portion, Vascular aurantii 24 weight portion, Herb Polygoni Chinensis 30 weight portion, Partition in Fructus Juglandis 32 weight portion, Radix Clematidis 22 weight portion, Receptaculum fici Henryi 12 weight portion, Cortex Albiziae 30 weight portion, RHIZOMA POLYGONI SUFFULTI 15 weight portion, Radix Gentianae Macrophyllae 33 weight portion, shepherd's purse 33 weight portion, Radix Cynanchi Paniculati 30 weight portion, Folium Callicarpae Formosanae 18 weight portion and Radix Glycyrrhizae 32 weight portion.
Described pharmaceutical composition also includes: Bulbus Tulipae, Rhizoma Ligustici, Folium Isatidis and Herba Senecionis Dianthui.
nullDescribed pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 14-23 weight portion、Folium paulowniae 15-35 weight portion、Radix Ampelopsis 15-30 weight portion、Endoconcha Sepiae 15-25 weight portion、Radix Angelicae Sinensis 15-40 weight portion、Folium Broussonetiae 18-26 weight portion、Chestnut 12-30 weight portion、Radix Cissi hexangularis 15-25 weight portion、White residual flower 15-40 weight portion、Vascular aurantii 12-24 weight portion、Herb Polygoni Chinensis 13-30 weight portion、Partition in Fructus Juglandis 16-32 weight portion、Radix Clematidis 12-25 weight portion、Receptaculum fici Henryi 10-20 weight portion、Cortex Albiziae 15-30 weight portion、RHIZOMA POLYGONI SUFFULTI 12-20 weight portion、Radix Gentianae Macrophyllae 15-35 weight portion、Shepherd's purse 15-34 weight portion、Radix Cynanchi Paniculati 15-32 weight portion、Folium Callicarpae Formosanae 14-22 weight portion、Radix Glycyrrhizae 15-35 weight portion、Bulbus Tulipae 3-5 weight portion、Rhizoma Ligustici 12-24 weight portion、Folium Isatidis 15-35 weight portion and Herba Senecionis Dianthui 10-20 weight portion.
Described pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 22 weight portion, Folium paulowniae 32 weight portion, Radix Ampelopsis 25 weight portion, Endoconcha Sepiae 18 weight portion, Radix Angelicae Sinensis 40 weight portion, Folium Broussonetiae 25 weight portion, chestnut 16 weight portion, Radix Cissi hexangularis 24 weight portion, white residual flower 40 weight portion, Vascular aurantii 24 weight portion, Herb Polygoni Chinensis 30 weight portion, Partition in Fructus Juglandis 32 weight portion, Radix Clematidis 22 weight portion, Receptaculum fici Henryi 12 weight portion, Cortex Albiziae 30 weight portion, RHIZOMA POLYGONI SUFFULTI 15 weight portion, Radix Gentianae Macrophyllae 33 weight portion, shepherd's purse 33 weight portion, Radix Cynanchi Paniculati 30 weight portion, Folium Callicarpae Formosanae 18 weight portion, Radix Glycyrrhizae 32 weight portion, Bulbus Tulipae 3 weight portion, Rhizoma Ligustici 24 weight portion, Folium Isatidis 30 weight portion and Herba Senecionis Dianthui 15 weight portion.
The dosage form of described pharmaceutical composition is injectable powder, and its preparation method comprises the following steps:
The first step, described raw medicinal material is mixed in proportion, put into the mixed powder being ground into the micron particles of 0.1～10 μm volume average particle size and particle diameter in super micron mill less than the nano-scale particle composition of 0.1 μm volume average particle size, described mixed powder powder rate is at least 95%；
Second step, the ethanol that determining alcohol is 85%～95% of the quality 3 relative to described mixed powder～5 times is added in the mixed powder that the first step obtains, stirring and dissolving obtains ethanol solution, ethanol solution is stood 24～36 hours under conditions of 5 DEG C～10 DEG C, use percolation with per minute 1～2ml speed slowly percolation, collect percolate, it is concentrated and dried, and pulverizing 40～60 minutes in super micron mill are put at this, the micron particles and the particle diameter that obtain 0.1～10 μm volume average particle size are less than the mixed powder that the nano-scale particle of 0.1 μm volume average particle size forms, the powder rate that obtains of described mixed powder is at least 95%；
3rd step, takes mixed powder 10g～20g that second step obtains, and adds propylene glycol 30g～50g, adds 200ml water for injection, and stirring makes it dissolve, injects water to 1000ml further, add 1g～1.5g needle-use activated carbon, be sufficiently stirred for 30～50 minutes；Decarbonization filtering；With 0.22～0.24 μm filtering with microporous membrane；Lyophilization obtains sterilized powder, subpackage embedding.
The dosage form of pharmaceutical composition described in the dosage form of described pharmaceutical composition is powder, and its preparation method comprises the following steps:
The first step, Radix Clematidis, Receptaculum fici Henryi, Cortex Albiziae, RHIZOMA POLYGONI SUFFULTI, Radix Gentianae Macrophyllae, shepherd's purse, Radix Cynanchi Paniculati, Folium Callicarpae Formosanae and Radix Glycyrrhizae are mixed in described ratio, dissolve with the ethanol that determining alcohol is 85%～95% of 3～5 times of the mixture quality relative to acquisition, extract after being heated to reflux 2～4 hours, filter, obtain the first filtrate；Filtering residue is dissolved with the ethanol of the determining alcohol 85%～95% of 1～2 times of the mixture quality relative to acquisition again, extracts after being heated to reflux 1～2 hour, filter, obtain the second filtrate；Merge the first filtrate and the second filtrate, decompression recycling ethanol to be concentrated into liquor strength be 0.7～0.9g crude drug/mL, through the macroporous adsorptive resins eluting that volume is 5L, first with deionized water or the distilled water eluting of 5 times of resin column volumes, it is 90%～95% ethanol elution with the determining alcohol of 3 times of resin column volumes again, collecting ethanol elution, concentrating under reduced pressure removes ethanol, is dried and is ground into powder；
Second step, Herba Lycopodii, Folium paulowniae, Radix Ampelopsis and Endoconcha Sepiae are mixed in described ratio, add the ethanol of determining alcohol 85%～95% relative to 2～4 times of mixture quality obtained, heating and refluxing extraction 1～2 times, extracting solution merges, being centrifuged remove impurity with tube centrifuge, when the extracting solution after remove impurity is evaporated under conditions of 60 DEG C～70 DEG C 60 DEG C, relative density is the mastic of 1.20～1.23, and the mastic spray dryer after concentrating is dried to powder；
3rd step, is mixed surplus stock medical material in described ratio, adds the water dissolution relative to 2～4 times of mixture quality obtained, and heating decocts 2～4 hours, filters；By filtering residue again with the water dissolution of 1～2 times of the mixture quality relative to acquisition, again heating decoction 1～2 hour, filtration；Merging the filtrate of twice, concentrating under reduced pressure removes moisture, is dried and is ground into powder；
4th step, the powder mixing that the first step, second step and the 3rd step are obtained, it is screened into the fine powder of 80～120 mesh subsequently, the fine powder obtained is put in mixing and blending machine and stirs, i.e. obtain powder.
The invention has the beneficial effects as follows: the medicine of the present invention has, at treatment cat tetanus tool, the advantage that cure rate is high, curative effect is short, by determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs, can strengthen cat immunity, reduce mortality rate, cost price is cheap.Medicine, in terms for the treatment of cat tetanus disease, has Western medicine and does not has the advantage that.
Detailed description of the invention
The invention provides a kind of preparation method for treating the tetanic pharmaceutical composition of cat, including following raw medicinal material: Herba Lycopodii, Folium paulowniae, Radix Ampelopsis, Endoconcha Sepiae, Rhizoma Ligustici, Radix Angelicae Sinensis, Folium Isatidis, Folium Broussonetiae, chestnut, Radix Cissi hexangularis, Bulbus Tulipae, white residual flower, Vascular aurantii, Herb Polygoni Chinensis, Partition in Fructus Juglandis, Radix Clematidis, Receptaculum fici Henryi, Herba Senecionis Dianthui, Cortex Albiziae, RHIZOMA POLYGONI SUFFULTI, Radix Gentianae Macrophyllae, shepherd's purse, Radix Cynanchi Paniculati, Folium Callicarpae Formosanae and Radix Glycyrrhizae.
Wherein, the pharmacology of above-mentioned raw materials medical material is as follows:
Radix Glycyrrhizae: source: this product is glycyrrhizic legume GlycyrrhizauralensisFisch., Glycyrrhiza inflata Bat. GlycyrrhizainflataBat. or the dry root of Glycyrrhiza glabra L. GlycyrrhizaglabraL..Spring, season in autumn two excavate, and remove fibrous root, dry.Nature and flavor: sweet are flat.Gui Jing: GUIXIN, lung, spleen, stomach warp.Cure mainly: invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.For weakness of the spleen and stomach, fatigue and weakness, shortness of breath and palpitation, cough with copious phlegm, gastral cavity abdomen, extremity contraction urgency pain, carbuncle sore tumefacting virus, cushion toxicity, strong.
Radix Cynanchi Paniculati: another name: Radix Cynanchi Paniculati, carefree bamboo, distant bamboo are free and unfettered, precious jade Garcinia mangostana, Radix Cynanchi Paniculati, to joint lotus, Folium Bambusae Herba Asari, bronze gong grass, Herba Veronicae, Herba sopubiae Trifidae.Source: this product is trailing plants section Cynanchum plant Radix Cynanchi Paniculati Cynanchumpaniculatum(Bge.) the dry root and rhizome of Kitag..Autumn excavates, and removes impurity, dries in the shade.Nature and flavor: pungent, temperature.Gui Jing: return liver, stomach warp.Cure mainly: removing dampness of dispeling the wind, antalgesic-antipruritic.For rheumatic arthralgia, distension of having a stomachache, toothache, lumbago, injury from falling down, urticaria, eczema.
Vascular aurantii: source: this product is the grain in the peel of rutaceae orange CitrusreticulataBlanco.Torn by peel, dry.Nature and flavor: bitter are flat.Cure mainly: dredging collateral, reduce phlegm.For cough with copious phlegm, the breast side of body is had a pain.
Radix Gentianae Macrophyllae: another name: gentiana straminea, Gentiana dahurica, Radix Gentianae Marcrophyllae, western Radix Gentianae Marcrophyllae, left torsion, left twist, RADIX GENTIANAE MACROPHYLLAE, RADIX GENTIANAE MACROPHYLLAE, Radix Raphani Macrophylla, plait Macrophylla.Source: this product is gentianaceae plant Radix Gentianae Macrophyllae GentianamacrophyllaPall., gentiana straminea maxim GentianastramineaMaxim., gentiana crassicaulis Duthie GentianacrassicaulisDuthieexBurk. or the dry root of Gentiana dahurica GentianadahuricaFisch..First three practises title " Radix Gentianae Macrophyllae " and " gentiana straminea " respectively by character difference, and latter is practised and claimed " Gentiana dahurica ".Spring, season in autumn two excavate, and remove silt；Radix Gentianae Macrophyllae and gentiana straminea shine soft, when " diaphoresis " to surface of banking up is reddish yellow or lark, spreads out and dry, or directly dry without " diaphoresis "；Rub with the hands when Gentiana dahurica is taken advantage of fresh and remove casting skin, dry.Nature and flavor: pungent, bitter are flat.Gui Jing: return stomach, liver, gallbladder meridian.Cure mainly: wind-damp dispelling, clearing away damp-heat, stopping numbness pain.For rheumatic arthralgia, muscle arteries and veins contracture, joint aches, and afternoon fever, infantile malnutrition generates heat.
Herba Senecionis Dianthui: source: Compositae Senecio plant Senecio dianthus SeneeiodianthusFranch., with all herbal medicine.Gathering herb autumn, cutting is dried.Nature and flavor: bitter, cold.Cure mainly: heat-clearing and toxic substances removing, wind-damp dispelling, antipruritic.For acute conjunctivitis, furuncle, dermatitis, traumatic injury.
White residual flower: another name: Flos rosae multiflorae.Source: Flos Rosae Multiflorae actions and spoken parts in classical Chinese drama residual flower RosamultifloraThunb.var.cathayensisRehd.etWils., is used as medicine with flower, root.Nature and flavor: bitter, puckery, cold.Cure mainly: flower: clearing away summer-heat heat, removing dampness is turbid, pleasant stomach function regulating.Cure mainly summer-heat uncomfortable in chest, thirsty, vomiting, anorexia, aphtha erosion of the oral mucosa.Consumption 1～3 money.Root: promoting blood circulation to remove obstruction in the collateral.Cure mainly arthritis, facial paralysis.Consumption 0.5～1 liang, be decocted in water for oral dose；External is ground into powder and can be controlled scald.
Radix Clematidis: another name: radix smilacis sieboldi, Radix Stemonae, Tacca chantrieri Andre, Herba Lespedezae Cuneatae.Source: this product is ranunculaceae plant Radix Clematidis ClematischinensisOsbeck, Clematis hexapetala (mountain knotweed) ClematishexapetalaPall. or the dry root and rhizome of northeast clematis (black common vetch) ClematismanshuricaRupr..Autumn excavates, and removes silt, dries.Nature and flavor: pungent, salty, temperature.Gui Jing: return bladder warp.Cure mainly: expelling wind and removing dampness, removing obstruction in the collateral to relieve pain.For rheumatic arthralgia, numb limbs and tense tendons, muscle arteries and veins contracture, joint stuffiness, bone is choked with sobs larynx.
Herb Polygoni Chinensis: source: " Bencao Tujing ".Another name: Linesless charcoal hair (" the SHENGCAO property of medicine is standby to be wanted "), crow charcoal (Lengthy Compilation of Plants with Illustrations), fortune medicine (" classifying herbal "), Polygonum aubertii grass (" Fujian traditional herbal medicine "), Ramulus et folium berchemiae floribundae, dizzy medicine (" Traditional Chinese Medicine in Sichuan will "), crow plain rice grass (" Quanzhou book on Chinese herbal medicine "), Linesless charcoal star, magpie sugar prunus mume (sieb.) sieb.et zucc. (" south of the Five Ridges herbal "), Herba Polygoni cymosi, crow meal rattan, water sand mandarin orange, Gu Ci meal, water move back acute diseases such as cholera and sunstroke (" Fujian Chinese herbal medicine "), Fructus Mume cotyledon, plain rice grass, great Ye sandy beach (" Guangdong Chinese herbal medicine "), mouse sugarcane (" Guangxi Chinese herbal medicine ").Source: for the herb of polygonaceae plant Herb Polygoni Chinensis.Summer, autumn gather, and dry.Nature and flavor: acid is sweet, cool.1. " Bencao Tujing ": " sour in the mouth, flat, nontoxic." 2. " product remittance essence is wanted ": " sour in the mouth is sweet, and property is put down, nontoxic." 3. " detailed outline ": " acid, flat, poisonous." 4. " the SHENGCAO property of medicine is standby to be wanted ": " sour in the mouth, cold in nature." 5. " Guangxi Chinese herbal medicine ": " micro-acid of taste is micro-puckery, slightly cool in nature." cure mainly: clearing away heat-damp and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body.Control and have loose bowels, dysentery, jaundice, wind heat pharynx pain, weak dizzy, summer non-acclimation in children, shy and jerk, women leucorrhea, carbuncle eczema, traumatic injury.
Cortex Albiziae: nature and flavor: sweet, flat.Gui Jing: GUIXIN, liver, lung meridian.Cure mainly: resolving stagnation for tranquilization, promoting blood circulation and detumescence.For irritability, melancholy insomnia, lung abscess skin ulcer swells, falls and flutter the pain of injury.
Bulbus Tulipae: source: " Henan Chinese medicine handbook ".Source: for the bulb of liliaceous plant Tulipa edulis.Spring, autumn, season in winter three all can gather.Take bulb, clean, remove fibrous root and crust, dry.Place the place of being dried to preserve.Nature and flavor: sweet, cold, poisonous.1. " Zhiwu Mingshi Tukao ": " sweet in the mouth, warm in nature." 2. " south of the Five Ridges gather medicinal herbs record ": " slightly sweet flavor, property is put down." 3. " Hunan medicine will ": " is sweet, cold, slightly poisonous." cure mainly: eliminating stagnation, blood stasis dispelling.Controlling laryngopharynx swelling and pain, scrofula, carbuncle, skin ulcer swells, and puerperal, the stasis of blood was stagnant.
Chestnut: source: " detailed outline ".Source: for the endocarp of Fagaceae chestnut.Nature and flavor: " detailed outline ": " sweet, flat, puckery, nontoxic." cure mainly: control scrofula, a fishbone or other bone caught in the throat.
Radix Angelicae Sinensis: source: this product is umbelliferae angelica Angelicasinensis(Oliv.) dry root of Diels.Autumn end excavates, and removes fibrous root and silt, after moisture slightly evaporates, is bundled into wisp, and upper canopy, with pyrotechnics slowly smoke-dried beancurd.Nature and flavor: sweet, acrid, warm.Gui Jing: return liver, the heart, spleen channel.Cure mainly: replenishing and activating blood, menstruction regulating and pain relieving, loosening bowel to relieve constipation.For blood deficiency and yellow complexion, dizziness cardiopalmus, menoxenia, amenorrhea dysmenorrhea, asthenia cold abdominalgia, dryness of the intestine constipation, rheumatic arthralgia, injury from falling down, ulcer sores.Radix Angelicae Sinensis (processed with wine) promoting blood circulation to restore menstrual flow.For amenorrhea dysmenorrhea, rheumatic arthralgia, injury from falling down.
Endoconcha Sepiae: another name: Os Sepiae, cuttlebone.Source: this product is the dry inner shell of Sepiidae animal sepiella maindroni de Rochebrune SepiellamaindronideRochebrune or golden cuttlefish SepiaesculentaHoyle.Collect the bone-shaped inner shell of sepiellae seu sepiae fish, cleaning, drying.Nature and flavor: salty, puckery, temperature.Gui Jing: return spleen, kidney channel.Cure mainly: astringing to arrest bleeding, relieving leukorrhea by astringents, antacid, sore.For acid regurgitation of having a stomachache, hematemesis and epistaxis, metrorrhagia has blood in stool, emission and spermatorrhea, leucorrhea with red and white discharge；Peptic Ulcers.External treatment bleeding due to trauma, the many pus of skin ulcer.
Folium Isatidis: source: this product is the dried leaves of cruciferae isatis IsatisindigoticaFort..In the summer, divide in season in autumn two 2～3 times and gather, remove impurity, dry.Nature and flavor: bitter, cold.Gui Jing: GUIXIN, stomach warp.Cure mainly: heat-clearing and toxic substances removing, blood cooling and ecchymoses removing.Join the army for pathogenic warmth, unconsciousness due to high fever, send out speckle dermexanthesis, jaundice, hematodiarrhoea, mumps, sore throat, erysipelas, carbuncle.
Folium Callicarpae Formosanae: another name: Folium Callicarpae Formosanae, Folium Callicarpae Formosanae.Source: Verenaceae Callicarpa plant callicarpa pedunculata CallicarpapedunculataR.Brown and Callicarpa dichotoma C.dichotoma(Lour.) K.Koch., is used as medicine with stem, leaf and root.Spring, summer, autumn picking leaves and tender stem, using fresh herb or dry is ground into powder；The root four seasons can adopt, and section is dried.Nature and flavor: bitter, puckery are flat.Cure mainly: hemostasis, dissipating blood stasis, antiinflammatory.For epistaxis, spitting of blood, gastrointestinal hemorrhage, metrorrhagia, upper respiratory tract infection, tonsillitis, pneumonia, bronchitis；Traumatic hemorrhage is controlled in external, burn.
Folium paulowniae: source: " Fujian traditional herbal medicine ".Another name: red Aeschna melanictera leaf (" illustrated handbook planted by middle traditional Chinese medicines ").Source: for the leaf of Verbenaceae paulownia.Nature and flavor: " Fujian traditional herbal medicine ": " sweet acid is micro-puckery, flat." cure mainly: detumescence dissipating blood stasis.Control carbuncle furuncle, traumatic injury.1. " Fujian traditional herbal medicine ": " promoting pus discharge and removing toxic substances, granulation promoting holds back mouth." 2. " Guangxi conventional medical herbs among the people ": " controls traumatic injury, innominate toxic swelling."
Partition in Fructus Juglandis: another name: Semen Juglandis kind every.Source: this product is the wooden barrier film of juglandaceae plant Semen Juglandis JuglansregiaL..Nature and flavor: bitter, puckery are flat.Cure mainly: the kidney invigorating arresting seminal emission.For emission due to deficiency of the kidney, spermatorrhea, the enuresis.
Rhizoma Ligustici: another name: Rhizoma Ligustici.Source: this product is Umbelliferae Ligustrum plant Rhizoma Ligustici LigusticumsinenseOliv. or the dry rhizome of Rhizoma Ligustici LigusticumjeholenseNakaietKitag. and root.The stem and leaf withered or secondary spring in autumn excavates when emerging, and removes silt, dries or dry.Nature and flavor: pungent, temperature.Gui Jing: return bladder warp.Cure mainly: dispel the wind, cold expelling, dehumidifying, pain relieving.For anemofrigid cold, top, mountain peak pain, rheumatism podomere arthralgia pain.
Radix Cissi hexangularis: another name: square-stem Herba Lycopodii, wing stem Cissus repens.Source: Vitaceae Cissus plant Radix Cissi hexangularis CissushexangularisThorelexPlanch., is used as medicine with rattan.The whole year can adopt, and dries or using fresh herb.Nature and flavor: micro-hardship are cool.Cure mainly: dispelling, collateral-activating, removing blood stasis and activating blood flow.For rheumatic arthritis, lumbar muscle strain, traumatic injury.
Herba Lycopodii: another name: Lycopodium clavatum, lion tail, lion hair careless, green stretch muscle, little stretch muscle, Lycopodium casuarinoides Spring.Source: this product is the dry herb of lycopsid Lycopodium clavatum LycopodiumjaponicumThunb..Summer, season in autumn two stem and leaf luxuriant time gather, remove impurity, dry.Nature and flavor: micro-hardship, pungent, temperature.Gui Jing: return liver,spleen,kidney warp.Cure mainly: expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral.
Shepherd's purse: source: " not Lu ".Another name:, (" elegant "), Fructus Pruni ginseng (" Bencao Tujing "), Folium Pruni Radix Adenophorae (Radix Glehniae), flour root (herbal for Relief of Famines), sweet Radix Platycodonis (" detailed outline "), soil Radix Platycodonis (" book on Chinese herbal medicine is original "), RADIX ADENOPHORAE (" Bencao Congxin "), Mei Can, the Radix Adenophorae (Radix Glehniae) that comes into leaves (" Zhejiang conventional medical herbs among the people ").Source: for the root of Campanulaceae shepherd's purse.Nature and flavor: sweet, cold.1. " not Lu ": " sweet, cold." 2. " a thousand pieces of gold wing book on Chinese herbal medicine ": " is nontoxic." 3. " Bencao Congxin ": " is sweet light, is slightly cold." return through: 1. " must join book on Chinese herbal medicine ": " enters meridian of Hand-TAI YIN." 2. " book on Chinese herbal medicine is looked for the truth ": " enters lung, spleen." cure mainly: heat clearing away, removing toxic substances, reduce phlegm.Control cough caused by dryness, laryngalgia, quench one's thirst, furuncle swelling toxin.
Receptaculum fici Henryi: source: come from 1. " Jiangxi medical herbs ".Another name: cliffstone pomegranate, Shi Pengpeng, little Fructus Fici Pumilae, Fructus Fici Pumilae, Ba Lizi, precipice pear, milk Pu, len-til, precipice Fructus Litchi, rock Folium Eriobotryae, joint hinge.Source: medical material Ji Yuan: for the fruit of moraceae plants Radix Thalictri Trichopi.Latin plant animal mineral name: FicussamentosaBuch.-Ham.exJ.E.Smithvar.Henryi(KingexOliv .) and Corner [F.henryiKingexOliv. " gather and store: autumn picking fruit, dries.Nature and flavor: sweet puckery；Flat；Nontoxic.Gui Jing: lung meridian.Cure mainly: reducing swelling and alleviating pain；Hemostasis.The main testis swelling and hanging down of either of the testes；Traumatic injury；Internal pile hematochezia.
RHIZOMA POLYGONI SUFFULTI: another name: Radix Euphorbiae [Hubei, Sichuan], screw Radix Notoginseng, Streptopus simplex D. Don, Rhizoma Polygoni sinensis, Herba Polygoni Runcinati [Guizhou], knotweed seven, Rhizoma Polygoni suffulti, Herba hyperici attenuati [Shaanxi].Source: for Polygonaceae arsesmart RHIZOMA POLYGONI SUFFULTI PolygonumsuffultumMaxim., be used as medicine with root stock.Autumn excavates, and cleans, and section is dried standby.Nature and flavor: bitter, puckery are cool.Cure mainly: astringing to arrest bleeding, pain relieving granulation promoting.For traumatic injury, traumatic hemorrhage, have blood in stool, metrorrhagia, dysentery, proctoptosis.
Radix Ampelopsis: another name: mountain region melon, wild Radix Ipomoeae, Vitis Amurensis seedling, Bletilla striata (Thunb.ex A.Murray)Rchb.f., Herba Rubi blinii.Source: this product is vitaceae Radix Ampelopsis Ampelopsisjaponica(Thunb.) dried root of Makino.Spring, season in autumn two excavate, and remove silt and radicula, are cut into vertical lobe or inclined tab, dry.Nature and flavor: bitter, are slightly cold.Gui Jing: GUIXIN, stomach warp.Cure mainly: heat-clearing and toxic substances removing, eliminating carbuncle eliminating stagnation.For carbuncle carbuncle on the back, furuncle, scrofula, burn due to hot liquid or fire.
Folium Broussonetiae: source: " not Lu ".Another name: structure leaf (" the secret record of primary and secondary "), Broussonetia papyrifera leaf (" easy folk prescription "), beans Huang leaf (" the SHENGCAO property of medicine is standby to be wanted ").Source: for the leaf of moraceae plants Broussonetia papyrifera.Nature and flavor: sweet are cool.1. " not Lu ": " sweet in the mouth is nontoxic." 2. " Japan hanako materia medica ": " is cool, nontoxic." 3. " the SHENGCAO property of medicine is standby to be wanted ": " taste is robbed, warm in nature." cure mainly: removing heat from blood, diuretic.Control haematemesis, epistaxis, metrorrhagia, traumatic hemorrhage, edema, hernia, dysentery, tinea skin ulcer.1. " not Lu ": " main children's's fever of the body eats not granulation promoting, exercises bath soup, the most main malignant boil raw meat." 2. " property of medicine opinion ": " is fried end and is searched face work food, main water dysentery." 3. " Japan hanako materia medica ": " controls thorn wind itching over the body." 4. " book on Chinese herbal medicine warp ": " main serious epistaxis of leaf.Tender shoots to eat when dish, main extremity migratory arthralgia, red white dysentery." 5. " detailed outline ": " diuresis, curing rheumatism swelling, nebulousurine, hernia, tinea skin ulcer." 6. " book on Chinese herbal medicine converges and says ": " removing heat from blood, dispels the wind, diuretic." 7. " book on Chinese herbal medicine asks former ": " leaf and root bark, He Yingwei, harness the river wet, under only collapsing." 8. " south of the Five Ridges gather medicinal herbs record ": " goes out abnormal pigmentary deposit on the skin acute diseases such as cholera and sunstroke." 9. " modern Practical Chinese medicinal ": " is beaten juice and is coated with insect poison and dermatosis."
nullDescribed pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 12-24 weight portion、Folium paulowniae 15-35 weight portion、Radix Ampelopsis 14-32 weight portion、Endoconcha Sepiae 13-27 weight portion、Radix Angelicae Sinensis 15-40 weight portion、Folium Broussonetiae 16-28 weight portion、Chestnut 12-32 weight portion、Radix Cissi hexangularis 15-25 weight portion、White residual flower 14-42 weight portion、Vascular aurantii 12-24 weight portion、Herb Polygoni Chinensis 13-31 weight portion、Partition in Fructus Juglandis 16-33 weight portion、Radix Clematidis 12-26 weight portion、Receptaculum fici Henryi 10-20 weight portion、Cortex Albiziae 14-32 weight portion、RHIZOMA POLYGONI SUFFULTI 11-21 weight portion、Radix Gentianae Macrophyllae 14-36 weight portion、Shepherd's purse 15-35 weight portion、Radix Cynanchi Paniculati 16-32 weight portion、Folium Callicarpae Formosanae 12-24 weight portion and Radix Glycyrrhizae 15-35 weight portion.
nullDescribed pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 14-23 weight portion、Folium paulowniae 15-35 weight portion、Radix Ampelopsis 15-30 weight portion、Endoconcha Sepiae 15-25 weight portion、Radix Angelicae Sinensis 15-40 weight portion、Folium Broussonetiae 18-26 weight portion、Chestnut 12-30 weight portion、Radix Cissi hexangularis 15-25 weight portion、White residual flower 15-40 weight portion、Vascular aurantii 12-24 weight portion、Herb Polygoni Chinensis 13-30 weight portion、Partition in Fructus Juglandis 16-32 weight portion、Radix Clematidis 12-25 weight portion、Receptaculum fici Henryi 10-20 weight portion、Cortex Albiziae 15-30 weight portion、RHIZOMA POLYGONI SUFFULTI 12-20 weight portion、Radix Gentianae Macrophyllae 15-35 weight portion、Shepherd's purse 15-34 weight portion、Radix Cynanchi Paniculati 15-32 weight portion、Folium Callicarpae Formosanae 14-22 weight portion and Radix Glycyrrhizae 15-35 weight portion.
Described pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 22 weight portion, Folium paulowniae 32 weight portion, Radix Ampelopsis 25 weight portion, Endoconcha Sepiae 18 weight portion, Radix Angelicae Sinensis 40 weight portion, Folium Broussonetiae 25 weight portion, chestnut 16 weight portion, Radix Cissi hexangularis 24 weight portion, white residual flower 40 weight portion, Vascular aurantii 24 weight portion, Herb Polygoni Chinensis 30 weight portion, Partition in Fructus Juglandis 32 weight portion, Radix Clematidis 22 weight portion, Receptaculum fici Henryi 12 weight portion, Cortex Albiziae 30 weight portion, RHIZOMA POLYGONI SUFFULTI 15 weight portion, Radix Gentianae Macrophyllae 33 weight portion, shepherd's purse 33 weight portion, Radix Cynanchi Paniculati 30 weight portion, Folium Callicarpae Formosanae 18 weight portion and Radix Glycyrrhizae 32 weight portion.
nullDescribed pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 14-23 weight portion、Folium paulowniae 15-35 weight portion、Radix Ampelopsis 15-30 weight portion、Endoconcha Sepiae 15-25 weight portion、Radix Angelicae Sinensis 15-40 weight portion、Folium Broussonetiae 18-26 weight portion、Chestnut 12-30 weight portion、Radix Cissi hexangularis 15-25 weight portion、White residual flower 15-40 weight portion、Vascular aurantii 12-24 weight portion、Herb Polygoni Chinensis 13-30 weight portion、Partition in Fructus Juglandis 16-32 weight portion、Radix Clematidis 12-25 weight portion、Receptaculum fici Henryi 10-20 weight portion、Cortex Albiziae 15-30 weight portion、RHIZOMA POLYGONI SUFFULTI 12-20 weight portion、Radix Gentianae Macrophyllae 15-35 weight portion、Shepherd's purse 15-34 weight portion、Radix Cynanchi Paniculati 15-32 weight portion、Folium Callicarpae Formosanae 14-22 weight portion、Radix Glycyrrhizae 15-35 weight portion、Bulbus Tulipae 3-5 weight portion、Rhizoma Ligustici 12-24 weight portion、Folium Isatidis 15-35 weight portion and Herba Senecionis Dianthui 10-20 weight portion.
Described pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 22 weight portion, Folium paulowniae 32 weight portion, Radix Ampelopsis 25 weight portion, Endoconcha Sepiae 18 weight portion, Radix Angelicae Sinensis 40 weight portion, Folium Broussonetiae 25 weight portion, chestnut 16 weight portion, Radix Cissi hexangularis 24 weight portion, white residual flower 40 weight portion, Vascular aurantii 24 weight portion, Herb Polygoni Chinensis 30 weight portion, Partition in Fructus Juglandis 32 weight portion, Radix Clematidis 22 weight portion, Receptaculum fici Henryi 12 weight portion, Cortex Albiziae 30 weight portion, RHIZOMA POLYGONI SUFFULTI 15 weight portion, Radix Gentianae Macrophyllae 33 weight portion, shepherd's purse 33 weight portion, Radix Cynanchi Paniculati 30 weight portion, Folium Callicarpae Formosanae 18 weight portion, Radix Glycyrrhizae 32 weight portion, Bulbus Tulipae 3 weight portion, Rhizoma Ligustici 24 weight portion, Folium Isatidis 30 weight portion and Herba Senecionis Dianthui 15 weight portion.
The dosage form of described pharmaceutical composition is injectable powder, and its preparation method comprises the following steps:
The first step, described raw medicinal material is mixed in proportion, put into the mixed powder being ground into the micron particles of 0.1～10 μm volume average particle size and particle diameter in super micron mill less than the nano-scale particle composition of 0.1 μm volume average particle size, described mixed powder powder rate is at least 95%；
Second step, the ethanol that determining alcohol is 85%～95% of the quality 3 relative to described mixed powder～5 times is added in the mixed powder that the first step obtains, stirring and dissolving obtains ethanol solution, ethanol solution is stood 24～36 hours under conditions of 5 DEG C～10 DEG C, use percolation with per minute 1～2ml speed slowly percolation, collect percolate, it is concentrated and dried, and pulverizing 40～60 minutes in super micron mill are put at this, the micron particles and the particle diameter that obtain 0.1～10 μm volume average particle size are less than the mixed powder that the nano-scale particle of 0.1 μm volume average particle size forms, the powder rate that obtains of described mixed powder is at least 95%；
3rd step, takes mixed powder 10g～20g that second step obtains, and adds propylene glycol 30g～50g, adds 200ml water for injection, and stirring makes it dissolve, injects water to 1000ml further, add 1g～1.5g needle-use activated carbon, be sufficiently stirred for 30～50 minutes；Decarbonization filtering；With 0.22～0.24 μm filtering with microporous membrane；Lyophilization obtains sterilized powder, subpackage embedding.
The dosage form of pharmaceutical composition described in the dosage form of described pharmaceutical composition is powder, and its preparation method comprises the following steps:
The first step, Radix Clematidis, Receptaculum fici Henryi, Cortex Albiziae, RHIZOMA POLYGONI SUFFULTI, Radix Gentianae Macrophyllae, shepherd's purse, Radix Cynanchi Paniculati, Folium Callicarpae Formosanae and Radix Glycyrrhizae are mixed in described ratio, dissolve with the ethanol that determining alcohol is 85%～95% of 3～5 times of the mixture quality relative to acquisition, extract after being heated to reflux 2～4 hours, filter, obtain the first filtrate；Filtering residue is dissolved with the ethanol of the determining alcohol 85%～95% of 1～2 times of the mixture quality relative to acquisition again, extracts after being heated to reflux 1～2 hour, filter, obtain the second filtrate；Merge the first filtrate and the second filtrate, decompression recycling ethanol to be concentrated into liquor strength be 0.7～0.9g crude drug/mL, through the macroporous adsorptive resins eluting that volume is 5L, first with deionized water or the distilled water eluting of 5 times of resin column volumes, it is 90%～95% ethanol elution with the determining alcohol of 3 times of resin column volumes again, collecting ethanol elution, concentrating under reduced pressure removes ethanol, is dried and is ground into powder；
Second step, Herba Lycopodii, Folium paulowniae, Radix Ampelopsis and Endoconcha Sepiae are mixed in described ratio, add the ethanol of determining alcohol 85%～95% relative to 2～4 times of mixture quality obtained, heating and refluxing extraction 1～2 times, extracting solution merges, being centrifuged remove impurity with tube centrifuge, when the extracting solution after remove impurity is evaporated under conditions of 60 DEG C～70 DEG C 60 DEG C, relative density is the mastic of 1.20～1.23, and the mastic spray dryer after concentrating is dried to powder；
3rd step, is mixed surplus stock medical material in described ratio, adds the water dissolution relative to 2～4 times of mixture quality obtained, and heating decocts 2～4 hours, filters；By filtering residue again with the water dissolution of 1～2 times of the mixture quality relative to acquisition, again heating decoction 1～2 hour, filtration；Merging the filtrate of twice, concentrating under reduced pressure removes moisture, is dried and is ground into powder；
4th step, the powder mixing that the first step, second step and the 3rd step are obtained, it is screened into the fine powder of 80～120 mesh subsequently, the fine powder obtained is put in mixing and blending machine and stirs, i.e. obtain powder.
Hereinafter using embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the process that realizes reaching technique effect can fully understand and implement according to this.
Embodiment 1 pharmaceutical composition 1
Embodiments provide a kind of for treating the tetanic pharmaceutical composition of cat, it is made up of the raw medicinal material of following parts by weight: take Herba Lycopodii 22 weight portion, Folium paulowniae 32 weight portion, Radix Ampelopsis 25 weight portion, Endoconcha Sepiae 18 weight portion, Radix Angelicae Sinensis 40 weight portion, Folium Broussonetiae 25 weight portion, chestnut 16 weight portion, Radix Cissi hexangularis 24 weight portion, white residual flower 40 weight portion, Vascular aurantii 24 weight portion, Herb Polygoni Chinensis 30 weight portion, Partition in Fructus Juglandis 32 weight portion, Radix Clematidis 22 weight portion, Receptaculum fici Henryi 12 weight portion, Cortex Albiziae 30 weight portion, RHIZOMA POLYGONI SUFFULTI 15 weight portion, Radix Gentianae Macrophyllae 33 weight portion, shepherd's purse 33 weight portion, Radix Cynanchi Paniculati 30 weight portion, Folium Callicarpae Formosanae 18 weight portion, Radix Glycyrrhizae 32 weight portion, Bulbus Tulipae 3 weight portion, Rhizoma Ligustici 24 weight portion, Folium Isatidis 30 weight portion and Herba Senecionis Dianthui 15 weight portion.
Its preparation method is as follows:
The first step, Radix Clematidis, Receptaculum fici Henryi, Cortex Albiziae, RHIZOMA POLYGONI SUFFULTI, Radix Gentianae Macrophyllae, shepherd's purse, Radix Cynanchi Paniculati, Folium Callicarpae Formosanae and Radix Glycyrrhizae are mixed in described ratio, dissolve with the ethanol that determining alcohol is 85%～95% of 3～5 times of the mixture quality relative to acquisition, extract after being heated to reflux 2～4 hours, filter, obtain the first filtrate；Filtering residue is dissolved with the ethanol of the determining alcohol 85%～95% of 1～2 times of the mixture quality relative to acquisition again, extracts after being heated to reflux 1～2 hour, filter, obtain the second filtrate；Merge the first filtrate and the second filtrate, decompression recycling ethanol to be concentrated into liquor strength be 0.7～0.9g crude drug/mL, through the macroporous adsorptive resins eluting that volume is 5L, first with deionized water or the distilled water eluting of 5 times of resin column volumes, it is 90%～95% ethanol elution with the determining alcohol of 3 times of resin column volumes again, collecting ethanol elution, concentrating under reduced pressure removes ethanol, is dried and is ground into powder；
Second step, Herba Lycopodii, Folium paulowniae, Radix Ampelopsis and Endoconcha Sepiae are mixed in described ratio, add the ethanol of determining alcohol 85%～95% relative to 2～4 times of mixture quality obtained, heating and refluxing extraction 1～2 times, extracting solution merges, being centrifuged remove impurity with tube centrifuge, when the extracting solution after remove impurity is evaporated under conditions of 60 DEG C～70 DEG C 60 DEG C, relative density is the mastic of 1.20～1.23, and the mastic spray dryer after concentrating is dried to powder；
3rd step, is mixed surplus stock medical material in described ratio, adds the water dissolution relative to 2～4 times of mixture quality obtained, and heating decocts 2～4 hours, filters；By filtering residue again with the water dissolution of 1～2 times of the mixture quality relative to acquisition, again heating decoction 1～2 hour, filtration；Merging the filtrate of twice, concentrating under reduced pressure removes moisture, is dried and is ground into powder；
4th step, the powder mixing that the first step, second step and the 3rd step are obtained, it is screened into the fine powder of 80～120 mesh subsequently, the fine powder obtained is put in mixing and blending machine and stirs, i.e. obtain powder.
Embodiment 2 pharmaceutical composition 2
Embodiments provide a kind of for treating the tetanic pharmaceutical composition of cat, it is made up of the raw medicinal material of following parts by weight: take Herba Lycopodii 22 weight portion, Folium paulowniae 32 weight portion, Radix Ampelopsis 25 weight portion, Endoconcha Sepiae 18 weight portion, Radix Angelicae Sinensis 40 weight portion, Folium Broussonetiae 25 weight portion, chestnut 16 weight portion, Radix Cissi hexangularis 24 weight portion, white residual flower 40 weight portion, Vascular aurantii 24 weight portion, Herb Polygoni Chinensis 30 weight portion, Partition in Fructus Juglandis 32 weight portion, Radix Clematidis 22 weight portion, Receptaculum fici Henryi 12 weight portion, Cortex Albiziae 30 weight portion, RHIZOMA POLYGONI SUFFULTI 15 weight portion, Radix Gentianae Macrophyllae 33 weight portion, shepherd's purse 33 weight portion, Radix Cynanchi Paniculati 30 weight portion, Folium Callicarpae Formosanae 18 weight portion and Radix Glycyrrhizae 32 weight portion.
Its preparation method comprises the following steps:
The first step, described raw medicinal material is mixed in proportion, put into the mixed powder being ground into the micron particles of 0.1～10 μm volume average particle size and particle diameter in super micron mill less than the nano-scale particle composition of 0.1 μm volume average particle size, described mixed powder powder rate is at least 95%；
Second step, the ethanol that determining alcohol is 85%～95% of the quality 3 relative to described mixed powder～5 times is added in the mixed powder that the first step obtains, stirring and dissolving obtains ethanol solution, ethanol solution is stood 24～36 hours under conditions of 5 DEG C～10 DEG C, use percolation with per minute 1～2ml speed slowly percolation, collect percolate, it is concentrated and dried, and pulverizing 40～60 minutes in super micron mill are put at this, the micron particles and the particle diameter that obtain 0.1～10 μm volume average particle size are less than the mixed powder that the nano-scale particle of 0.1 μm volume average particle size forms, the powder rate that obtains of described mixed powder is at least 95%；
3rd step, takes mixed powder 10g～20g that second step obtains, and adds propylene glycol 30g～50g, adds 200ml water for injection, and stirring makes it dissolve, injects water to 1000ml further, add 1g～1.5g needle-use activated carbon, be sufficiently stirred for 30～50 minutes；Decarbonization filtering；With 0.22～0.24 μm filtering with microporous membrane；Lyophilization obtains sterilized powder, subpackage embedding.
Toxicity test
1, cumulative toxicity test report
Select 30 healthy mices, female, male each 10, with the pharmaceutical composition of the embodiment of the present invention 1 as object, dose design is a phase in every 7 days, each issue dosage is respectively 0.10LD50,0.20LD50,0.25LD50,0.30LD50,0.40LD50, the LD50 female, male mice of this sample be 4000m part/k part BW (five dosage is 500,750,1100,1800,2400m part/k part BW).Within every 7 days, weigh once, adjust dosage, by 0.05ml/10 part BW per os gavage.After 28 days, animal occurs without death condition, off-test, by ascending-dose to tested material 28 days after, female, buck is all without death condition generation.With the pharmaceutical composition of embodiment 2 as object, carrying out toxicity test according to the method described above, result such as embodiment 1 is identical.
2, acute toxicity test
Application NIH mice 40, SPF level, male and female half and half, body weight 15～20g, carry out acute toxicity test.Mice is randomly divided into two groups, often group 20, i.e. matched group and administration group, fasting 12 hours before experiment；Pharmaceutical composition prepared by embodiments of the invention 1 is dissolved in water, (concentration is 6.75g crude drug/ml, maximum concentration) gavage, gavage volume be 7ml/kg(i.e. unit dosage form be 28.7 crude drugs/kg), matched group gives normal saline, within one day, is administered 3 times, delivery time 8 hours, Continuous Observation 14 days after administration, and record toxic reaction and the death toll of mice.Test result indicate that: compare with matched group, after administration, mice has no notable difference, and experiment Continuous Observation 21 days, mouse systemic situation, diet, drinking-water, body weight increase the most normal.Therefore the acute toxicity of the medicine of the embodiment of the present invention 1 is low, clinical drug safety.Respectively with the pharmaceutical composition of embodiment 2 as object, carrying out toxicity test according to the method described above, result such as embodiment 1 is identical.
Clinical trial
Animal experiment uses the design of single factor test contrast test, takes 75 50 week old, body weight about 2.2, healthy domestic cat, male and female half and half, and test manages routinely in cat field and prevents, and carries out compiling overbit, anthelmintic and protective inoculation in preliminary trial period.
The just phase of trying is randomly divided into test group (test group is respectively embodiment 1 group, embodiment 2 groups) and matched group, often group 25.Test period is 60 days, and period free choice feeding is freely drunk water, and carries out immunity and anthelmintic routinely.
Feed as follows:
Matched group: basal diet；
Test group:
Embodiment 1 group: basal diet+1.5% medicine；Said medicine is the pharmaceutical composition 1 that the embodiment of the present invention 1 prepares；
Embodiment 2 groups: basal diet+1.0% medicine；Said medicine is the pharmaceutical composition 2 that the embodiment of the present invention 2 prepares；
Test period is 60 days, prepares feeding capacity and clout amount during record test, calculates every cat average daily ingestion amount weekly and full phase average daily ingestion amount；On-test and at the end of morning 7:30 weigh on an empty stomach, calculate the average daily gain of every cat；Carry out the health status record of routine work record and cat.All data use SPSS13.0 software kit to carry out variance analysis and Duncan ' S multiple comparisons.Result represents with mean+SD.
To embodiment 1 group, embodiment 2 groups and the cat of matched group, gather 10mL sky venous blood, take 2mL heparin anti-coagulating for t lymphocyte subset group (CD3, CD4, CD8) quantity, the mensuration of lymphocyte positive rate.The serum separated is used for measuring interleukin (IL-2), gamma interferon content.Lymphocyte positive rate uses acid-α-naphthalene acetate esterase enzyme Determination Staining.IL-2, gamma interferon content are detected by ELISA method, and IL-2, gamma interferon test kit are provided by content MARKET company of the U.S..Use the quantity of U.S.'s Coulter company EPICS-XL flow cytomery T lymphocyte subsets.
The impact on cat tetanus immunological function of table 1 medicine
Project Matched group Embodiment 1 group Embodiment 2 groups
P(IL-2)(pg/ml) 4.20±1.32 5.12±1.77 4.92±1.31
P (gamma interferon) (pg/ml) 35.72±3.65 49.4±4.02 48.3±1.70
Lymphocyte positive rate (%) 54.47±3.21 60.7±4.05 61.1±1.64
CD3(%) 57.43±5.12 34.2±3.25 34.1±1.58
CD4(%) 31.16±3.17 36.2±1.44 36.3±1.13
CD8(%) 21.57±1.97 21.2±0.35 21.3±1.42
CD4/CD8(%) 1.44±0.004 1.7±0.007 1.7±0.004
As it can be seen from table 1 the cat of drug treating group is compared with matched group, the IL-2 in serum, gamma interferon, lymphocyte positive rate, CD4, CD4/CD8 value all increase.As can be seen here, the medicine of the present invention improves CD4(T in cat immunocyte and assists cell) and CD4(T assist cell) suppress cell with CD8(T) and ratio, increase the compositions such as the complement of serum, IL-2, gamma interferon, improve innate immune activity in cat body to a certain extent, strengthen the immunity of cat body.
Treatment contrast test:
In October ,-2014 in October, 2013,300 domestic cats are had to suffer from tetanus, 300 cats are randomly divided into 4 groups, it is respectively embodiment 1 group, embodiment 2 groups and western medicine group, the pharmaceutical composition 2 using pharmaceutical composition 1 that embodiment 1 prepares, embodiment 2 to prepare respectively carries out treating and conventional medicament is treated, wherein, the dose that embodiment is 1 group-2 be every every day 0.3g, be 10-20 days during treatment；The conventional medicament of western medicine group selects tetanus antitoxin 10mg/mL, tetanus immune globulin 15mg/mL to carry out neck injection, every day 1 time, treats 10-20 days.Treatment is observed three months after terminating, and records cure rate and the mortality rate of each group of cat, and result is as shown in table 1.
Table 1 each experimental group therapeutic effect synopsis
Group Ill cat number (only) Death toll Mortality rate Cure number Cure rate
Embodiment 1 group 100 0% 0% 100 100%
Embodiment 2 groups 100 2% 2% 98 98%
Western medicine group 100 24% 24% 76 76%
Knowable to the data of table 1: pharmaceutical composition of the present invention therapeutic effect tetanic to cat is substantially better than western medicine group, reduce mortality rate, improve cure rate.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.

Claims (9)

1. one kind is used for treating the tetanic pharmaceutical composition of cat, it is characterized in that, described pharmaceutical composition is made up of following raw medicinal material: Herba Lycopodii, Folium paulowniae, Radix Ampelopsis, Endoconcha Sepiae, Radix Angelicae Sinensis, Folium Broussonetiae, chestnut, Radix Cissi hexangularis, white residual flower, Vascular aurantii, Herb Polygoni Chinensis, Partition in Fructus Juglandis, Radix Clematidis, Receptaculum fici Henryi, Cortex Albiziae, RHIZOMA POLYGONI SUFFULTI, Radix Gentianae Macrophyllae, shepherd's purse, Radix Cynanchi Paniculati, Folium Callicarpae Formosanae and Radix Glycyrrhizae.
nullThe treatment tetanic pharmaceutical composition of cat the most according to claim 1，It is characterized in that，Described pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 12-24 weight portion、Folium paulowniae 15-35 weight portion、Radix Ampelopsis 14-32 weight portion、Endoconcha Sepiae 13-27 weight portion、Radix Angelicae Sinensis 15-40 weight portion、Folium Broussonetiae 16-28 weight portion、Chestnut 12-32 weight portion、Radix Cissi hexangularis 15-25 weight portion、White residual flower 14-42 weight portion、Vascular aurantii 12-24 weight portion、Herb Polygoni Chinensis 13-31 weight portion、Partition in Fructus Juglandis 16-33 weight portion、Radix Clematidis 12-26 weight portion、Receptaculum fici Henryi 10-20 weight portion、Cortex Albiziae 14-32 weight portion、RHIZOMA POLYGONI SUFFULTI 11-21 weight portion、Radix Gentianae Macrophyllae 14-36 weight portion、Shepherd's purse 15-35 weight portion、Radix Cynanchi Paniculati 16-32 weight portion、Folium Callicarpae Formosanae 12-24 weight portion and Radix Glycyrrhizae 15-35 weight portion.
nullThe most according to claim 1 and 2 for treating the tetanic pharmaceutical composition of cat，It is characterized in that，Described pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 14-23 weight portion、Folium paulowniae 15-35 weight portion、Radix Ampelopsis 15-30 weight portion、Endoconcha Sepiae 15-25 weight portion、Radix Angelicae Sinensis 15-40 weight portion、Folium Broussonetiae 18-26 weight portion、Chestnut 12-30 weight portion、Radix Cissi hexangularis 15-25 weight portion、White residual flower 15-40 weight portion、Vascular aurantii 12-24 weight portion、Herb Polygoni Chinensis 13-30 weight portion、Partition in Fructus Juglandis 16-32 weight portion、Radix Clematidis 12-25 weight portion、Receptaculum fici Henryi 10-20 weight portion、Cortex Albiziae 15-30 weight portion、RHIZOMA POLYGONI SUFFULTI 12-20 weight portion、Radix Gentianae Macrophyllae 15-35 weight portion、Shepherd's purse 15-34 weight portion、Radix Cynanchi Paniculati 15-32 weight portion、Folium Callicarpae Formosanae 14-22 weight portion and Radix Glycyrrhizae 15-35 weight portion.
4. treat the tetanic pharmaceutical composition of cat according to being used for described in any one of claim 1-3, it is characterized in that, described pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 22 weight portion, Folium paulowniae 32 weight portion, Radix Ampelopsis 25 weight portion, Endoconcha Sepiae 18 weight portion, Radix Angelicae Sinensis 40 weight portion, Folium Broussonetiae 25 weight portion, chestnut 16 weight portion, Radix Cissi hexangularis 24 weight portion, white residual flower 40 weight portion, Vascular aurantii 24 weight portion, Herb Polygoni Chinensis 30 weight portion, Partition in Fructus Juglandis 32 weight portion, Radix Clematidis 22 weight portion, Receptaculum fici Henryi 12 weight portion, Cortex Albiziae 30 weight portion, RHIZOMA POLYGONI SUFFULTI 15 weight portion, Radix Gentianae Macrophyllae 33 weight portion, shepherd's purse 33 weight portion, Radix Cynanchi Paniculati 30 weight portion, Folium Callicarpae Formosanae 18 weight portion and Radix Glycyrrhizae 32 weight portion.
5. treat the tetanic pharmaceutical composition of cat according to arbitrary described being used for of claim 1-4, it is characterised in that described pharmaceutical composition also includes: Bulbus Tulipae, Rhizoma Ligustici, Folium Isatidis and Herba Senecionis Dianthui.
null6. treat the tetanic pharmaceutical composition of cat according to arbitrary described being used for of claim 1-5，It is characterized in that，Described pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 14-23 weight portion、Folium paulowniae 15-35 weight portion、Radix Ampelopsis 15-30 weight portion、Endoconcha Sepiae 15-25 weight portion、Radix Angelicae Sinensis 15-40 weight portion、Folium Broussonetiae 18-26 weight portion、Chestnut 12-30 weight portion、Radix Cissi hexangularis 15-25 weight portion、White residual flower 15-40 weight portion、Vascular aurantii 12-24 weight portion、Herb Polygoni Chinensis 13-30 weight portion、Partition in Fructus Juglandis 16-32 weight portion、Radix Clematidis 12-25 weight portion、Receptaculum fici Henryi 10-20 weight portion、Cortex Albiziae 15-30 weight portion、RHIZOMA POLYGONI SUFFULTI 12-20 weight portion、Radix Gentianae Macrophyllae 15-35 weight portion、Shepherd's purse 15-34 weight portion、Radix Cynanchi Paniculati 15-32 weight portion、Folium Callicarpae Formosanae 14-22 weight portion、Radix Glycyrrhizae 15-35 weight portion、Bulbus Tulipae 3-5 weight portion、Rhizoma Ligustici 12-24 weight portion、Folium Isatidis 15-35 weight portion and Herba Senecionis Dianthui 10-20 weight portion.
null7. treat the tetanic pharmaceutical composition of cat according to arbitrary described being used for of claim 1-6，It is characterized in that，Described pharmaceutical composition is made up of the raw medicinal material of following parts by weight: Herba Lycopodii 22 weight portion、Folium paulowniae 32 weight portion、Radix Ampelopsis 25 weight portion、Endoconcha Sepiae 18 weight portion、Radix Angelicae Sinensis 40 weight portion、Folium Broussonetiae 25 weight portion、Chestnut 16 weight portion、Radix Cissi hexangularis 24 weight portion、White residual flower 40 weight portion、Vascular aurantii 24 weight portion、Herb Polygoni Chinensis 30 weight portion、Partition in Fructus Juglandis 32 weight portion、Radix Clematidis 22 weight portion、Receptaculum fici Henryi 12 weight portion、Cortex Albiziae 30 weight portion、RHIZOMA POLYGONI SUFFULTI 15 weight portion、Radix Gentianae Macrophyllae 33 weight portion、Shepherd's purse 33 weight portion、Radix Cynanchi Paniculati 30 weight portion、Folium Callicarpae Formosanae 18 weight portion、Radix Glycyrrhizae 32 weight portion、Bulbus Tulipae 3 weight portion、Rhizoma Ligustici 24 weight portion、Folium Isatidis 30 weight portion and Herba Senecionis Dianthui 15 weight portion.
8. according to the preparation method for treating the tetanic pharmaceutical composition of cat described in claim 1-7, it is characterised in that the dosage form of described pharmaceutical composition is injectable powder, and its preparation method comprises the following steps:
The first step, described raw medicinal material is mixed in proportion, put into the mixed powder being ground into the micron particles of 0.1～10 μm volume average particle size and particle diameter in super micron mill less than the nano-scale particle composition of 0.1 μm volume average particle size, described mixed powder powder rate is at least 95%；
Second step, the ethanol that determining alcohol is 85%～95% of the quality 3 relative to described mixed powder～5 times is added in the mixed powder that the first step obtains, stirring and dissolving obtains ethanol solution, ethanol solution is stood 24～36 hours under conditions of 5 DEG C～10 DEG C, use percolation with per minute 1～2ml speed slowly percolation, collect percolate, it is concentrated and dried, and pulverizing 40～60 minutes in super micron mill are put at this, the micron particles and the particle diameter that obtain 0.1～10 μm volume average particle size are less than the mixed powder that the nano-scale particle of 0.1 μm volume average particle size forms, the powder rate that obtains of described mixed powder is at least 95%；
3rd step, takes mixed powder 10g～20g that second step obtains, and adds propylene glycol 30g～50g, adds 200ml water for injection, and stirring makes it dissolve, injects water to 1000ml further, add 1g～1.5g needle-use activated carbon, be sufficiently stirred for 30～50 minutes；Decarbonization filtering；With 0.22～0.24 μm filtering with microporous membrane；Lyophilization obtains sterilized powder, subpackage embedding.
9. according to the preparation method for treating the tetanic pharmaceutical composition of cat described in claim 1-7, it is characterised in that the dosage form of pharmaceutical composition described in the dosage form of described pharmaceutical composition is powder, and its preparation method comprises the following steps:
The first step, Radix Clematidis, Receptaculum fici Henryi, Cortex Albiziae, RHIZOMA POLYGONI SUFFULTI, Radix Gentianae Macrophyllae, shepherd's purse, Radix Cynanchi Paniculati, Folium Callicarpae Formosanae and Radix Glycyrrhizae are mixed in described ratio, dissolve with the ethanol that determining alcohol is 85%～95% of 3～5 times of the mixture quality relative to acquisition, extract after being heated to reflux 2～4 hours, filter, obtain the first filtrate；Filtering residue is dissolved with the ethanol of the determining alcohol 85%～95% of 1～2 times of the mixture quality relative to acquisition again, extracts after being heated to reflux 1～2 hour, filter, obtain the second filtrate；Merge the first filtrate and the second filtrate, decompression recycling ethanol to be concentrated into liquor strength be 0.7～0.9g crude drug/mL, through the macroporous adsorptive resins eluting that volume is 5L, first with deionized water or the distilled water eluting of 5 times of resin column volumes, it is 90%～95% ethanol elution with the determining alcohol of 3 times of resin column volumes again, collecting ethanol elution, concentrating under reduced pressure removes ethanol, is dried and is ground into powder；
Second step, Herba Lycopodii, Folium paulowniae, Radix Ampelopsis and Endoconcha Sepiae are mixed in described ratio, add the ethanol of determining alcohol 85%～95% relative to 2～4 times of mixture quality obtained, heating and refluxing extraction 1～2 times, extracting solution merges, being centrifuged remove impurity with tube centrifuge, when the extracting solution after remove impurity is evaporated under conditions of 60 DEG C～70 DEG C 60 DEG C, relative density is the mastic of 1.20～1.23, and the mastic spray dryer after concentrating is dried to powder；
3rd step, is mixed surplus stock medical material in described ratio, adds the water dissolution relative to 2～4 times of mixture quality obtained, and heating decocts 2～4 hours, filters；By filtering residue again with the water dissolution of 1～2 times of the mixture quality relative to acquisition, again heating decoction 1～2 hour, filtration；Merging the filtrate of twice, concentrating under reduced pressure removes moisture, is dried and is ground into powder；
4th step, the powder mixing that the first step, second step and the 3rd step are obtained, it is screened into the fine powder of 80～120 mesh subsequently, the fine powder obtained is put in mixing and blending machine and stirs, i.e. obtain powder.
CN201610384924.5A 2016-06-02 2016-06-02 Pharmaceutical composition for treating cat tetanus and preparing method thereof Withdrawn CN105816635A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103845628A (en) * 2014-04-01 2014-06-11 山东新希望六和集团有限公司 Medicine for treating cat tetanus and preparation method of medicine
CN104873947A (en) * 2015-06-17 2015-09-02 张建国 Medicinal composition for treating dog acute upper gastrointestinal hemorrhage and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103845628A (en) * 2014-04-01 2014-06-11 山东新希望六和集团有限公司 Medicine for treating cat tetanus and preparation method of medicine
CN104873947A (en) * 2015-06-17 2015-09-02 张建国 Medicinal composition for treating dog acute upper gastrointestinal hemorrhage and preparation method thereof

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Application publication date: 20160803