CN105767801A - Immune regulation type radix pseudostellariae functional beverage and preparation method thereof - Google Patents

Immune regulation type radix pseudostellariae functional beverage and preparation method thereof Download PDF

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Publication number
CN105767801A
CN105767801A CN201610140495.7A CN201610140495A CN105767801A CN 105767801 A CN105767801 A CN 105767801A CN 201610140495 A CN201610140495 A CN 201610140495A CN 105767801 A CN105767801 A CN 105767801A
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radix pseudostellariae
sucrose
juice
water
drinks
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贾强
王芳
李永霞
徐青
武玉祥
韩瑜
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Guizhou Institute of Biology
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Guizhou Institute of Biology
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/56Flavouring or bittering agents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/72Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
    • A23L2/74Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration using membranes, e.g. osmosis, ultrafiltration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Water Supply & Treatment (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

The present invention relates to the technical field of functional beverage manufacturing and particularly relates to an immune regulation type radix pseudostellariae functional beverage and a preparation method thereof. The radix pseudostellariae beverage is prepared from the raw materials of radix pseudostellariae juice, apple juice, acids, sucrose, high fructose corn syrup, salt, traditional Chinese medicinal extract, a suspension stabilizer, ganoderma lucidum polysaccharides, taurine and flavor. The apple juice and the radix pseudostellariae juice interact with each other to reach an efficacy of improving mouthfeel. By combining the functions of the ganoderma lucidum polysaccharides, taurine and radix pseudostellariae, the beverage has efficacies of improving immunity and enhancing health-care function. Especially the traditional Chinese medicinal extract is a mixture of licorice and haws, the radix pseudostellariae juice, apple juice, traditional Chinese medicinal extract, ganoderma lucidum polysaccharides and taurine have a synergic efficacy, so that the nutritional value and health-care effects of the beverage are improved and the beverage has an efficacy of enhancing immunity.

Description

A kind of immunity regulating type Radix Pseudostellariae drinks and preparation method thereof
Technical field
The present invention relates to drinks manufacture technology field, especially a kind of immunity regulating type Radix Pseudostellariae drinks and preparation method thereof.
Background technology
Radix Pseudostellariae (English name RADIXPSEUDOSTELLARIAE) is the medical herbs that " Chinese Pharmacopoeia " includes, the dried root of medicinal source pinkwort Radix Ginseng (Pseudostellariaheterophylla (Miq.) PaxexPaxetHoffm.).Radix Pseudostellariae belongs to herbaceos perennial, the long spindle of tuber, white, slightly band sallow.Stem is upright, Dan Sheng, and blade falls lanceolar, and the blunt point in top, base portion is gradually narrow in long handle shape, and blade width egg shape or Pedicellus et Pericarpium Trapae shape are avette.Radix Pseudostellariae likes the environment being grown in warm and moist, be afraid of high temperature, be distributed in the ground such as East China, Central China, North China, northeast and northwest, medical material main product in Fujian, Guizhou, Jiangsu, Shandong, the ground such as Anhui, determined by Ministry of Public Health and listed " can be used for the Chinese crude drug list of health food " in.Radix Pseudostellariae hardship sweet, micro-, flat.Return spleen, lung meridian.Body lubricant nature and, tonifying QI to produce body fluid.
In prior art, the research that as raw material, Radix Pseudostellariae is applied to field of beverage is also relatively fewer, it is mainly reflected in Radix Pseudostellariae and some Chinese medicine extract, Flos Osmanthi Fragrantis etc. are as preparing some functional beverages after raw material, but owing to different raw materials is in carrying out the process mixed, the proportioning of both differs, mouthfeel of beverage is affected for it and the impact of nutritive value is all different, cause that the market prospect adopting Radix Pseudostellariae to be beverage prepared by raw material is poor, hinder exploitation and the application of Radix Pseudostellariae.
Summary of the invention
In order to solve the above-mentioned technical problem existed in prior art, the present invention provides a kind of immunity regulating type Radix Pseudostellariae drinks and preparation method thereof.
It is achieved particular by techniques below scheme:
A kind of immunity regulating type Radix Pseudostellariae drinks, its material composition with parts by weight be Radix Pseudostellariae juice 1-3 part, Sucus Mali pumilae 5-10 part, acid 0.3-0.5 part, sucrose 2-4 part, high fructose syrup 2-4 part, salt 0.02-0.04 part, Chinese medicine extraction liquid 20-30 part, suspension stabilizer 0.04-0.06 part, ganoderan 0.05-0.07 part, taurine 0.04-0.06 part, essence 0.01-0.02 part.
Described material composition with parts by weight be 2 parts of Radix Pseudostellariae juice, Sucus Mali pumilae 8 parts, acid 0.4 part, sucrose 3 parts, high fructose syrup 3 parts, salt 0.03 part, Chinese medicine extraction liquid 25 parts, suspension stabilizer 0.05 part, ganoderan 0.06 part, taurine 0.05 part, 0.015 part of essence.
Described material composition with parts by weight be 2 parts of Radix Pseudostellariae juice, Sucus Mali pumilae 7.5 parts, acid 0.4 part, sucrose 3 parts, high fructose syrup 3 parts, salt 0.03 part, Chinese medicine extraction liquid 25 parts, suspension stabilizer 0.05 part, ganoderan 0.06 part, taurine 0.05 part, 0.01 part of essence.
Described Chinese medicine extraction liquid its be Fructus Crataegi and the mixed extracting solution of Radix Glycyrrhizae.
Described Fructus Crataegi and the mixing ratio of Radix Glycyrrhizae for etc. mass mixing.
The preparation method also providing for immunity regulating type Radix Pseudostellariae drinks, comprise the following steps:
(1) select fresh Radix Pseudostellariae root, rinsed, drain, stripping and slicing, be placed in squeezer to squeeze into juice, and after being filtered, obtain Radix Pseudostellariae juice;Radix Pseudostellariae juice is mixed with Sucus Mali pumilae, obtains feed liquid;
(2) suspension stabilizer and sucrose are taken, and accounting for the quality of suspension stabilizer 0.1-3% according to sucrose, to carry out dry blending uniform, it is added thereto to the water of 75-85 DEG C again, the consumption of water is 1-3 times of quality of suspension stabilizer, stirring is dissolved to abundant, and without, after insoluble particulate matter, adding it in the feed liquid of step (1), stir, obtain just finished product;
(3) remaining in sucrose, high fructose syrup addition 75-85 DEG C in water, the consumption of water is 1-3 times of quality of sucrose, stirring extremely fully dissolving; join in first finished product; it is subsequently added ganoderan, taurine, Chinese medicine extraction liquid, salt, and stirs, obtain semi-finished product;
(4) water of acid 55-65 DEG C is fully dissolved, and add it to, in semi-finished product, stir;After again essence being added, temperature be 35-45 DEG C, pressure be that under 18-22MPa, homogenizing processes;The membrane filtration being adopted aperture to be 0.2 micron again is degerming, bottling, capping, labeling, is placed under the environment of 2-8 DEG C and preserves, obtains immunity regulating type Radix Pseudostellariae drinks.
Described acid is the mass mixing thing such as grade of citric acid and malic acid;Described salt is Sal.
Described step (3), it mixes in the process being added to the water dissolving at sucrose and high fructose syrup, and it has also been added thereto to sweet sugar TR100 as sweeting agent, and addition is the 1-3% of sucrose.
The preparation method also providing for immunity regulating type Radix Pseudostellariae drinks, comprises the following steps:
(1) select fresh Radix Pseudostellariae root, rinsed, drain, stripping and slicing, be placed in squeezer to squeeze into juice, and after being filtered, obtain Radix Pseudostellariae juice;Radix Pseudostellariae juice is mixed with Sucus Mali pumilae, obtains feed liquid;
(2) suspension stabilizer and sucrose are taken, and accounting for the quality of suspension stabilizer 0.1-3% according to sucrose, to carry out dry blending uniform, it is added thereto to the water of 80 DEG C again, the consumption of water is 1-3 times of quality of suspension stabilizer, stirring is dissolved to abundant, and without, after insoluble particulate matter, adding it in the feed liquid of step (1), stir, obtain just finished product;
(3) remaining in sucrose, high fructose syrup addition 80 DEG C in water, the consumption of water is 1-3 times of quality of sucrose, stirring extremely fully dissolving; join in first finished product; it is subsequently added ganoderan, taurine, Chinese medicine extraction liquid, salt, and stirs, obtain semi-finished product;
(4) acid is fully dissolved with the water of 60 DEG C, and add it to, in semi-finished product, stir;After again essence being added, temperature be 40 DEG C, pressure be that under 20MPa, homogenizing processes;The membrane filtration being adopted aperture to be 0.2 micron again is degerming, bottling, capping, labeling, is placed under the environment of 5 DEG C and preserves, obtains immunity regulating type Radix Pseudostellariae drinks.
Compared with prior art, the technique effect of the present invention is embodied in:
By making Radix Pseudostellariae beverage with Radix Pseudostellariae juice, Sucus Mali pumilae, acid, sucrose, high fructose syrup, salt, Chinese medicine extraction liquid, suspension stabilizer, ganoderan, taurine, essence for raw material; Sucus Mali pumilae is interacted with Radix Pseudostellariae juice; reach to improve effect of mouthfeel; in the effect in conjunction with ganoderan, taurine and Radix Pseudostellariae so that beverage vessel is improved immunity, strengthens the effect of health-care effect;Especially in conjunction with Chinese medicine extraction liquid adopts Radix Glycyrrhizae and Fructus Crataegi mixing; make to occur between Radix Pseudostellariae juice, Sucus Mali pumilae, Chinese medicine extraction liquid, ganoderan, taurine mutually collaborative effect; improve nutritive value and the health-care effect of beverage so that it has effect of enhancing immunity.
Process especially in combination with preparation technology so that the mouthfeel of the beverage of preparation and nutritive value are all more excellent, and adopt membrane filtration technique, it is therefore prevented that the impact that high temperature sterilize causes to the quality of beverage, it is to avoid nutrition leak, it is therefore prevented that brown stain.
The good stability of this beverage products, the shelf-life is longer, is depositing after more than 6 months, is finding no any layering and denaturalization phenomenon, and especially the content of vitamin will not occur to change largely;And its preparation technology is simple, with short production cycle, workable, it is easy to promote, promoted the fast development of Radix Pseudostellariae related industry.
Detailed description of the invention
Below in conjunction with specific embodiment technical scheme done further restriction, but the scope claimed is not only limited to the description made.
Embodiment 1
A kind of immunity regulating type Radix Pseudostellariae drinks, its material composition is Radix Pseudostellariae juice 1kg, Sucus Mali pumilae 5kg, acid 0.3kg, sucrose 2kg, high fructose syrup 2kg, salt 0.02kg, Chinese medicine extraction liquid 20kg, suspension stabilizer 0.04kg, ganoderan 0.05kg, taurine 0.04kg, essence 0.01kg by weight.
Described Chinese medicine extraction liquid its be Fructus Crataegi and the mixed extracting solution of Radix Glycyrrhizae.
Described Fructus Crataegi and the mixing ratio of Radix Glycyrrhizae for etc. mass mixing.
The preparation method of above-mentioned immunity regulating type Radix Pseudostellariae drinks, comprises the following steps:
(1) select fresh Radix Pseudostellariae root, rinsed, drain, stripping and slicing, be placed in squeezer to squeeze into juice, and after being filtered, obtain Radix Pseudostellariae juice;Radix Pseudostellariae juice is mixed with Sucus Mali pumilae, obtains feed liquid;
(2) suspension stabilizer and sucrose are taken, and accounting for the quality of suspension stabilizer 0.1% according to sucrose, to carry out dry blending uniform, it is added thereto to the water of 75 DEG C again, the consumption of water is 1 times of quality of suspension stabilizer, stirring is dissolved to abundant, and without, after insoluble particulate matter, adding it in the feed liquid of step (1), stir, obtain just finished product;
(3) remaining in sucrose, high fructose syrup addition 75 DEG C in water, the consumption of water is 1-3 times of quality of sucrose, stirring extremely fully dissolving; join in first finished product; it is subsequently added ganoderan, taurine, Chinese medicine extraction liquid, salt, and stirs, obtain semi-finished product;
(4) acid is fully dissolved with the water of 55 DEG C, and add it to, in semi-finished product, stir;After again essence being added, temperature be 35 DEG C, pressure be that under 18MPa, homogenizing processes;The membrane filtration being adopted aperture to be 0.2 micron again is degerming, bottling, capping, labeling, is placed under the environment of 2 DEG C and preserves, obtains immunity regulating type Radix Pseudostellariae drinks.
Described acid is the mass mixing thing such as grade of citric acid and malic acid;Described salt is Sal.
Described step (3), it mixes in the process being added to the water dissolving at sucrose and high fructose syrup, and it has also been added thereto to sweet sugar TR100 as sweeting agent, and addition is the 1% of sucrose.
Embodiment 2
A kind of immunity regulating type Radix Pseudostellariae drinks, its material composition is Radix Pseudostellariae juice 3kg, Sucus Mali pumilae 10kg, acid 0.5kg, sucrose 4kg, high fructose syrup 4kg, salt 0.04kg, Chinese medicine extraction liquid 30kg, suspension stabilizer 0.06kg, ganoderan 0.07kg, taurine 0.06kg, essence 0.02kg by weight.
Described Chinese medicine extraction liquid its be Fructus Crataegi and the mixed extracting solution of Radix Glycyrrhizae.
Described Fructus Crataegi and the mixing ratio of Radix Glycyrrhizae for etc. mass mixing.
The preparation method of above-mentioned immunity regulating type Radix Pseudostellariae drinks, comprises the following steps:
(1) select fresh Radix Pseudostellariae root, rinsed, drain, stripping and slicing, be placed in squeezer to squeeze into juice, and after being filtered, obtain Radix Pseudostellariae juice;Radix Pseudostellariae juice is mixed with Sucus Mali pumilae, obtains feed liquid;
(2) suspension stabilizer and sucrose are taken, and accounting for the quality of suspension stabilizer 3% according to sucrose, to carry out dry blending uniform, it is added thereto to the water of 85 DEG C again, the consumption of water is 3 times of quality of suspension stabilizer, stirring is dissolved to abundant, and without, after insoluble particulate matter, adding it in the feed liquid of step (1), stir, obtain just finished product;
(3) remaining in sucrose, high fructose syrup addition 85 DEG C in water, the consumption of water is 3 times of quality of sucrose, stirring extremely fully dissolving; join in first finished product; it is subsequently added ganoderan, taurine, Chinese medicine extraction liquid, salt, and stirs, obtain semi-finished product;
(4) acid is fully dissolved with the water of 65 DEG C, and add it to, in semi-finished product, stir;After again essence being added, temperature be 45 DEG C, pressure be that under 22MPa, homogenizing processes;The membrane filtration being adopted aperture to be 0.2 micron again is degerming, bottling, capping, labeling, is placed under the environment of 8 DEG C and preserves, obtains immunity regulating type Radix Pseudostellariae drinks.
Described acid is the mass mixing thing such as grade of citric acid and malic acid;Described salt is Sal.
Described step (3), it mixes in the process being added to the water dissolving at sucrose and high fructose syrup, and it has also been added thereto to sweet sugar TR100 as sweeting agent, and addition is the 3% of sucrose.
Embodiment 3
On the basis of embodiment 1; other are all with embodiment 1; a kind of immunity regulating type Radix Pseudostellariae drinks, its material composition is Radix Pseudostellariae juice 2kg, Sucus Mali pumilae 8kg, acid 0.4kg, sucrose 3kg, high fructose syrup 3kg, salt 0.03kg, Chinese medicine extraction liquid 25kg, suspension stabilizer 0.05kg, ganoderan 0.06kg, taurine 0.05kg, essence 0.015kg by weight.
Described acid is the mass mixing thing such as grade of citric acid and malic acid;Described salt is Sal.
The preparation method of above-mentioned immunity regulating type Radix Pseudostellariae drinks, comprises the following steps:
(1) select fresh Radix Pseudostellariae root, rinsed, drain, stripping and slicing, be placed in squeezer to squeeze into juice, and after being filtered, obtain Radix Pseudostellariae juice;Radix Pseudostellariae juice is mixed with Sucus Mali pumilae, obtains feed liquid;
(2) suspension stabilizer and sucrose are taken, and accounting for the quality of suspension stabilizer 2% according to sucrose, to carry out dry blending uniform, it is added thereto to the water of 80 DEG C again, the consumption of water is 2 times of quality of suspension stabilizer, stirring is dissolved to abundant, and without, after insoluble particulate matter, adding it in the feed liquid of step (1), stir, obtain just finished product;
(3) remaining in sucrose, high fructose syrup addition 80 DEG C in water, the consumption of water is 2 times of quality of sucrose, stirring extremely fully dissolving; join in first finished product; it is subsequently added ganoderan, taurine, Chinese medicine extraction liquid, salt, and stirs, obtain semi-finished product;
(4) acid is fully dissolved with the water of 60 DEG C, and add it to, in semi-finished product, stir;After again essence being added, temperature be 40 DEG C, pressure be that under 20MPa, homogenizing processes;The membrane filtration being adopted aperture to be 0.2 micron again is degerming, bottling, capping, labeling, is placed under the environment of 5 DEG C and preserves, obtains immunity regulating type Radix Pseudostellariae drinks.
Embodiment 4
On the basis of embodiment 2; other are all with embodiment 2; a kind of immunity regulating type Radix Pseudostellariae drinks, its material composition is Radix Pseudostellariae juice 2kg, Sucus Mali pumilae 7.5kg, acid 0.4kg, sucrose 3kg, high fructose syrup 3kg, salt 0.03kg, Chinese medicine extraction liquid 25kg, suspension stabilizer 0.05kg, ganoderan 0.06kg, taurine 0.05kg, essence 0.01kg by weight.
Described Chinese medicine extraction liquid its be Fructus Crataegi and the mixed extracting solution of Radix Glycyrrhizae.
Described Fructus Crataegi and the mixing ratio of Radix Glycyrrhizae for etc. mass mixing.
In order to prove the technique effect of the present invention, the present embodiment also provides for following experimental example.
One, the test medicine impact on immunologic hypofunction mice serum hemolysin
(1) material
1, laboratory animal: Kunming mouse, male and female half and half, body weight 18~22g, SPF level, Zhongshan University's Experimental Animal Center provide, the animal quality certification number: Guangdong probatio inspectionem pecuoarem word SCXK (Guangdong)-2014-0011.
2, medicine packet and process:
Normal group: distilled water.
Model group: cyclophosphamide lumbar injection modeling.
Test medicine low dose group: take the formula being equivalent to Radix Pseudostellariae beverage embodiment 1 before experiment, the dosage of 500ml ratio adds distilled water, and to be made into 250ml stand-by, each gavage 0.25ml of low dose group.
Dosage group in test medicine: take the formula being equivalent to Radix Pseudostellariae beverage embodiment 1 before experiment, the dosage of 500ml ratio adds distilled water, and to be made into 250ml stand-by, each gavage 0.5ml of low dose group.
Test medicine high dose group: take the formula being equivalent to Radix Pseudostellariae beverage embodiment 1 before experiment, the dosage of 500ml ratio adds distilled water, and to be made into 250ml stand-by, each gavage 1.0ml of low dose group.
Positive control medicine (paecilomyces hepiall chen) group: paecilomyces hepiall chen is produced by Jiangxi Jinshuibao Pharmaceutical Co., Ltd., lot number: 140907.Take paecilomyces hepiall chen content before experiment, be made into, with distilled water, the solution that concentration is 39mg/ml.
(2) reagent and instrument
Cyclophosphamide: Hualian Pharmaceutical Co., Ltd., Shanghai produces, lot number: 20140714.Physiology salt is used before experiment
Water is made into the solution that concentration is 5mg/ml.
Glucose: analytical pure, Guangzhou Chemical Reagent Factory produces, lot number: 20110601-3.
Sodium citrate: analytical pure, Guangzhou Chemical Reagent Factory produces, lot number: 20130904-1.
Citric acid: analytical pure, Guangzhou Chemical Reagent Factory produces, lot number: 20140404-1.
Sodium chloride: analytical pure, Shantou City's brilliance laboratory, lot number: 20131208.
Chicken erythrocyte suspension: venous blood collection under the chicken wing under aseptic condition, puts into Alsever ' the s liquid being equivalent to Sanguis Gallus domesticus volume 5 times, 4 DEG C of preservations.Face the used time and use normal saline cyclic washing, centrifugal 3 times, with constant hematocrit number normal saline be made into 5% chicken red blood cell suspension (Alsever ' preparation of s liquid: glucose 2.05g, sodium citrate 0.80g, citric acid 0.05g, sodium chloride 0.42g, double distilled water adds to 1.0L, autoclaving, 4 DEG C of preservations).
Guinea pig serum: take guinea pig whole blood by the method for Culling heart blood, is then centrifuged for obtaining serum.
Dual-beam ultraviolet-uisible spectrophotometer: Beijing Puxi General Instrument Co., Ltd, model: TU-1901.
Gastric perfusion needle: occupational health inspection center, Guangdong Province Experimental Animal Center customizes.
1ml syringe, small test tube, test tube rack.
(2) experimental situation
Illumination: common;Ventilate: common;Cleanliness factor: regular grade;Temperature: 20 DEG C ± 1 DEG C;Humidity: 50% ± 5%.
Laboratory animal environmental facility: occupational health inspection center, Guangdong Province Laboratory Animal Facility, the quality certification number: Guangdong probatio inspectionem pecuoarem word 2011C031.
(3) experimental technique
(1) Kunming mouse 72 is taken, body weight 18~22g, be randomly divided in Normal group, modeling group, test medicine low dose group, test medicine dosage group, test medicine high dose group, positive control medicine (paecilomyces hepiall chen) group totally 6 groups, often group 12.
(2) before experiment, water 10h is can't help in mice fasting, and then each group (except dosage group) mice presses 0.1ml/10g body weight gastric infusion, 1 time/d, altogether 7d, and wherein Normal group and cyclophosphamide modeling group are to equal-volume distilled water.
(3) except Normal group, all the other 10 groups, all in 3d to the 5d intraperitoneal injection of cyclophosphamide 50mg/kg of administration, are caused immunologic hypofunction model.
(4) at the test every Mus lumbar injection 5% chicken erythrocyte suspension 0.2ml of 1d, stimulating animal immunity.
(5) 1h after last administration, wins animal eyeball and takes blood, and 3000r/min is centrifuged 10min, separates serum, with normal saline by serum-dilution 100 times.
(6) dilute serum 1ml is taken, mix with 5% chicken erythrocyte suspension 0.5ml, 10% guinea pig serum (complement) 0.5ml, at 37 DEG C of water-bath incubation 30min, then stopped reaction in DEG C refrigerator is set to 0, by centrifugal for aforesaid liquid 3000r/min 10min, take supernatant, measure OD value with dual-beam ultraviolet-uisible spectrophotometer in 540nm place, separately set the blank pipe zeroing being not added with mice serum.Serum hemolysin is represented with the OD value of sample.
(4) experimental result
Experimental result is as shown in table 1:
Table 1
From table 1, test medicine high dose group serum hemolysin compares with cyclophosphamide modeling group, has significant difference (P < 0.05) statistically.(2) positive control medicine (paecilomyces hepiall chen) is organized serum hemolysin and is compared with cyclophosphamide modeling group, has significant differences (P < 0.01) but not statistically significant.
Two, the test medicine impact on the carbon clearance of immunologic hypofunction mice
(1) material
1, laboratory animal: Kunming mouse, male and female half and half, body weight 18~22g, SPF level, Zhongshan University's Experimental Animal Center provide, the animal quality certification number: Guangdong probatio inspectionem pecuoarem word SCXK (Guangdong)-2014-0011.
2, medicine packet and process:
Normal group: distilled water.
Model group: with cyclophosphamide lumbar injection modeling.
Test medicine low dose group: take the dosage being equivalent to Radix Pseudostellariae drink formula 1,500ml ratio before experiment and add distilled water to be made into 250ml stand-by, each gavage 0.25ml of low dose group.
Dosage group in test medicine: take the dosage being equivalent to Radix Pseudostellariae drink formula 1,500ml ratio before experiment and add distilled water to be made into 250ml stand-by, each gavage 0.5ml of low dose group.
Test medicine high dose group: take the dosage being equivalent to Radix Pseudostellariae drink formula 1,500ml ratio before experiment and add distilled water to be made into 250ml stand-by, each gavage 1.0ml of low dose group.
Positive control medicine (paecilomyces hepiall chen) group: paecilomyces hepiall chen is produced by Jiangxi Jinshuibao Pharmaceutical Co., Ltd., lot number: 140907.Take paecilomyces hepiall chen content before experiment, be made into, with distilled water, the solution that concentration is 39mg/ml.
3, reagent and instrument:
Cyclophosphamide: Hualian Pharmaceutical Co., Ltd., Shanghai produces, lot number: 20140714.Physiology salt is used before experiment
Water is made into the solution that concentration is 5mg/ml.
Sodium carbonate: analytical pure, Guangzhou Chemical Reagent Factory produces, lot number: 20110403-2.Test front distillation
Water is made into the sodium carbonate liquor that concentration is 0.1%.
Dual-beam ultraviolet-uisible spectrophotometer: Beijing Puxi General Instrument Co., Ltd, model: TU-1901.
Gastric perfusion needle: occupational health inspection center, Guangdong Province Experimental Animal Center customizes.
1ml syringe, small test tube, test tube rack.
(2) experimental situation
Illumination: common;Ventilate: common;Cleanliness factor: regular grade;Temperature: 20 DEG C ± 1 DEG C;Humidity: 50% ± 5%.
Laboratory animal environmental facility: occupational health inspection center, Guangdong Province Laboratory Animal Facility, the quality certification number: Guangdong probatio inspectionem pecuoarem word 2011C031.
(3) test method
Take Kunming mouse 72, body weight 18~22g, be randomly divided in Normal group, cyclophosphamide modeling group, test medicine low dose group, test medicine dosage group, test medicine high dose group, positive control medicine (paecilomyces hepiall chen) group totally 6 groups, often group 12, before experiment, water 10h is can't help in mice fasting, then mice (except dosage group) is respectively organized by 0.1ml/10g body weight gastric infusion, 1 time/d, 7d altogether, wherein Normal group and cyclophosphamide modeling group are to equal-volume distilled water.Except Normal group, all the other 10 groups, all in 3d to the 5d intraperitoneal injection of cyclophosphamide 50mg/kg of administration, are caused immunologic hypofunction model.1h after last administration, each mouse tail vein injection india ink 0.1ml/10g body weight, 2min (T after injection1)、12min(T2) take blood 20 μ l with the suction pipe of heparin sodium moistening from orbital vein respectively, it is dissolved in 0.1% sodium carbonate liquor of 2ml, shake up, with dual-beam ultraviolet-uisible spectrophotometer in 680nm wavelength place survey OD value, finally by mice sacrificed by exsanguination, take liver spleen and claim its quality, calculate and clean up index, correct and clean up index.
Clean up index K=(A1-㏒A2)/(T2-T1), A in formula1、A2The respectively OD value of 2min, 12min.
(4) experimental result
Through statistics, experimental result is in Table 2.
Table 2
Note: compare with cyclophosphamide modeling group:*P < 0.05;**P < 0.01.
From the experimental data of table 2, the middle and high dosage group correction of test medicine is cleaned up index and is compared with cyclophosphamide modeling group, has significant differences (P < 0.01) statistically.Positive control medicine paecilomyces hepiall chen group compares with cyclophosphamide modeling group, has significant differences (P < 0.01) statistically.
This immunity regulating type Radix Pseudostellariae drinks; make Radix Pseudostellariae, ganoderan and taurine nutrition and health care effectively be combined, improve Radix Pseudostellariae drink health-care effect, drink the clear order in back and feel well; often drink can enhancing immunity, be a kind of health promoting beverage sutable for men, women, and children.
Be necessary at this remind be; above example is only limitted to technical scheme is made further explanation; so that the understanding that those skilled in the art are to technical scheme; it it is not the restriction to technical scheme; those skilled in the art are on this basis; the essential characteristics of the non-protruding made and the improvement of non-significant progress, belong to the protection category of the present invention.

Claims (9)

1. an immunity regulating type Radix Pseudostellariae drinks; it is characterized in that, its material composition with parts by weight be Radix Pseudostellariae juice 1-3 part, Sucus Mali pumilae 5-10 part, acid 0.3-0.5 part, sucrose 2-4 part, high fructose syrup 2-4 part, salt 0.02-0.04 part, Chinese medicine extraction liquid 20-30 part, suspension stabilizer 0.04-0.06 part, ganoderan 0.05-0.07 part, taurine 0.04-0.06 part, essence 0.01-0.02 part.
2. immunity regulating type Radix Pseudostellariae drinks as claimed in claim 1; it is characterized in that, described material composition with parts by weight be 2 parts of Radix Pseudostellariae juice, Sucus Mali pumilae 8 parts, acid 0.4 part, sucrose 3 parts, high fructose syrup 3 parts, salt 0.03 part, Chinese medicine extraction liquid 25 parts, suspension stabilizer 0.05 part, ganoderan 0.06 part, taurine 0.05 part, 0.015 part of essence.
3. immunity regulating type Radix Pseudostellariae drinks as claimed in claim 1; it is characterized in that, described material composition with parts by weight be 2 parts of Radix Pseudostellariae juice, Sucus Mali pumilae 7.5 parts, acid 0.4 part, sucrose 3 parts, high fructose syrup 3 parts, salt 0.03 part, Chinese medicine extraction liquid 25 parts, suspension stabilizer 0.05 part, ganoderan 0.06 part, taurine 0.05 part, 0.01 part of essence.
4. the immunity regulating type Radix Pseudostellariae drinks as described in claim 1 or 2 or 3, it is characterised in that described Chinese medicine extraction liquid its be Fructus Crataegi and the mixed extracting solution of Radix Glycyrrhizae.
5. immunity regulating type Radix Pseudostellariae drinks as claimed in claim 4, it is characterised in that described Fructus Crataegi and the mixing ratio of Radix Glycyrrhizae for etc. mass mixing.
6. the preparation method of the immunity regulating type Radix Pseudostellariae drinks as described in any one of claim 1-5, it is characterised in that comprise the following steps:
(1) select fresh Radix Pseudostellariae root, rinsed, drain, stripping and slicing, be placed in squeezer to squeeze into juice, and after being filtered, obtain Radix Pseudostellariae juice;Radix Pseudostellariae juice is mixed with Sucus Mali pumilae, obtains feed liquid;
(2) suspension stabilizer and sucrose are taken, and accounting for the quality of suspension stabilizer 0.1-3% according to sucrose, to carry out dry blending uniform, it is added thereto to the water of 75-85 DEG C again, the consumption of water is 1-3 times of quality of suspension stabilizer, stirring is dissolved to abundant, and without, after insoluble particulate matter, adding it in the feed liquid of step (1), stir, obtain just finished product;
(3) remaining in sucrose, high fructose syrup addition 75-85 DEG C in water, the consumption of water is 1-3 times of quality of sucrose, stirring extremely fully dissolving; join in first finished product; it is subsequently added ganoderan, taurine, Chinese medicine extraction liquid, salt, and stirs, obtain semi-finished product;
(4) water of acid 55-65 DEG C is fully dissolved, and add it to, in semi-finished product, stir;After again essence being added, temperature be 35-45 DEG C, pressure be that under 18-22MPa, homogenizing processes;The membrane filtration being adopted aperture to be 0.2 micron again is degerming, bottling, capping, labeling, is placed under the environment of 2-8 DEG C and preserves, obtains immunity regulating type Radix Pseudostellariae drinks.
7. the preparation method of immunity regulating type Radix Pseudostellariae drinks as claimed in claim 6, it is characterised in that described acid is the mass mixing thing such as grade of citric acid and malic acid;Described salt is Sal.
8. the preparation method of immunity regulating type Radix Pseudostellariae drinks as claimed in claim 6, it is characterized in that, described step (3), it mixes in the process being added to the water dissolving at sucrose and high fructose syrup, it has also been added thereto to sweet sugar TR100 as sweeting agent, and addition is the 1-3% of sucrose.
9. the preparation method of immunity regulating type Radix Pseudostellariae drinks as claimed in claim 6, it is characterised in that comprise the following steps:
(1) select fresh Radix Pseudostellariae root, rinsed, drain, stripping and slicing, be placed in squeezer to squeeze into juice, and after being filtered, obtain Radix Pseudostellariae juice;Radix Pseudostellariae juice is mixed with Sucus Mali pumilae, obtains feed liquid;
(2) suspension stabilizer and sucrose are taken, and accounting for the quality of suspension stabilizer 0.1-3% according to sucrose, to carry out dry blending uniform, it is added thereto to the water of 80 DEG C again, the consumption of water is 1-3 times of quality of suspension stabilizer, stirring is dissolved to abundant, and without, after insoluble particulate matter, adding it in the feed liquid of step (1), stir, obtain just finished product;
(3) remaining in sucrose, high fructose syrup addition 80 DEG C in water, the consumption of water is 1-3 times of quality of sucrose, stirring extremely fully dissolving; join in first finished product; it is subsequently added ganoderan, taurine, Chinese medicine extraction liquid, salt, and stirs, obtain semi-finished product;
(4) acid is fully dissolved with the water of 60 DEG C, and add it to, in semi-finished product, stir;After again essence being added, temperature be 40 DEG C, pressure be that under 20MPa, homogenizing processes;The membrane filtration being adopted aperture to be 0.2 micron again is degerming, bottling, capping, labeling, is placed under the environment of 5 DEG C and preserves, obtains immunity regulating type Radix Pseudostellariae drinks.
CN201610140495.7A 2016-03-12 2016-03-12 Immune regulation type radix pseudostellariae functional beverage and preparation method thereof Pending CN105767801A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108740617A (en) * 2018-06-09 2018-11-06 河南九术医药科技有限公司 A kind of health beverages improving immunity

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1088793A (en) * 1992-12-28 1994-07-06 闽东制药厂 Health Pseudostellaria medicine
CN102488262A (en) * 2011-11-22 2012-06-13 杨军 Manufacturing method of beverage for strengthening immunities of children
CN103976436A (en) * 2014-04-25 2014-08-13 贵州三泓药业股份有限公司 Preparing process of radix pseudostellariae health care beverage
CN104273605A (en) * 2013-07-12 2015-01-14 陈洪涛 Apple juice capable of stimulating appetite and digestion
CN104522808A (en) * 2014-12-29 2015-04-22 通化百泉保健食品有限公司 Ginseng-radix pseudostellariae composite health drink and preparation method thereof
CN104664532A (en) * 2015-03-20 2015-06-03 杨素华 Radix pseudostellariae beverage and preparation method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1088793A (en) * 1992-12-28 1994-07-06 闽东制药厂 Health Pseudostellaria medicine
CN102488262A (en) * 2011-11-22 2012-06-13 杨军 Manufacturing method of beverage for strengthening immunities of children
CN104273605A (en) * 2013-07-12 2015-01-14 陈洪涛 Apple juice capable of stimulating appetite and digestion
CN103976436A (en) * 2014-04-25 2014-08-13 贵州三泓药业股份有限公司 Preparing process of radix pseudostellariae health care beverage
CN104522808A (en) * 2014-12-29 2015-04-22 通化百泉保健食品有限公司 Ginseng-radix pseudostellariae composite health drink and preparation method thereof
CN104664532A (en) * 2015-03-20 2015-06-03 杨素华 Radix pseudostellariae beverage and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
朱珠等: "《食品工艺学概论》", 31 March 2014, 郑州大学出版社 *
陈耀荣: "《黛眉山光明中医录》", 31 July 2015, 郑州大学出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108740617A (en) * 2018-06-09 2018-11-06 河南九术医药科技有限公司 A kind of health beverages improving immunity

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