CN105767013B - A kind of bactericidal composition of the activator protein containing toVerticilliumdahliaActivitie Activitie S of Relative - Google Patents
A kind of bactericidal composition of the activator protein containing toVerticilliumdahliaActivitie Activitie S of Relative Download PDFInfo
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- CN105767013B CN105767013B CN201610288679.8A CN201610288679A CN105767013B CN 105767013 B CN105767013 B CN 105767013B CN 201610288679 A CN201610288679 A CN 201610288679A CN 105767013 B CN105767013 B CN 105767013B
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- activator protein
- toverticilliumdahliaactivitie activitie
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- 108091006088 activator proteins Proteins 0.000 title claims abstract description 93
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- 241000700605 Viruses Species 0.000 claims description 21
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- 241000221785 Erysiphales Species 0.000 claims description 12
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- 239000004480 active ingredient Substances 0.000 claims 1
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- 229920002521 macromolecule Polymers 0.000 description 4
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- 108010059892 Cellulase Proteins 0.000 description 3
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 3
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- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 241000223602 Alternaria alternata Species 0.000 description 2
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- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000724205 Rice stripe tenuivirus Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
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- -1 antifreeze Substances 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
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- 239000003085 diluting agent Substances 0.000 description 1
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- 238000007667 floating Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Abstract
The invention discloses a kind of bactericidal compositions of activator protein containing toVerticilliumdahliaActivitie Activitie S of Relative, the bactericidal composition containing effective active components A and B, and active components A is toVerticilliumdahliaActivitie Activitie S of Relative activator protein, and active constituent B is selected from any one following compound：Chitosan, Portugal's polyene sugar, lentinan, oligosaccharide element, and active components A and the weight ratio of active constituent B are 1：500~500：1.The present composition has greater activity to the multiple diseases in various crop, and has apparent synergistic effect, expands fungicidal spectrum.And the characteristics of metabolic system with activated plant, promotion plant cell increase, and root activity is reinforced.
Description
The application is divisional application, and original application is that " one kind contains for the patent application of Shanxi Thompson Biological Technology Co., Ltd.
The bactericidal composition of toVerticilliumdahliaActivitie Activitie S of Relative activator protein ", the application Wei ︰ 2012105209955 of original application, original application day are
2012.12.04。
Technical field
The invention belongs to technical field of pesticide, it is related to a kind of bactericidal composition of the activator protein containing toVerticilliumdahliaActivitie Activitie S of Relative in crop
Application in disease.
Technical background
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein (plant activator protein) molecular formula：C963H1564O342N280S3, chemistry
Title：207 peptides proteins.ToVerticilliumdahliaActivitie Activitie S of Relative activator protein makes plant growth accelerate vigor by the metabolic system of activated plant
Enhancing, the increase of proline content and the reinforcement of cellulase promote plant cell to increase, and root activity is reinforced, and volume increase is reached
Purpose.
In the real process of agricultural production, the problem of controlling disease most easily produces is the generation of disease drug resistance.No
It is compounded with kind ingredient, is to prevent the very common method of resistance disease.Heterogeneity is compounded, and is imitated according to practical application
Fruit, to judge that certain compounding is synergy, adduction or antagonism.In most cases, the compounding effect of pesticide is all to add
And effect, really have the compounding of synergistic effect seldom, especially synergistic effect clearly, the very high compounding of synergy ratio just more
It is few.It studies, is found toVerticilliumdahliaActivitie Activitie S of Relative activator protein and chitosan, Portugal's polyene sugar, lentinan, oligomeric by inventor
Sugared element mutually compounding, there is good synergistic effect in a certain range, can endure the anti pathologic immunity power of high crop well, enhance root
The activity of system, to achieve the purpose that volume increase, and in relation to toVerticilliumdahliaActivitie Activitie S of Relative activator protein and chitosan, Portugal's polyene sugar, mushroom
Polysaccharide, the related compounding of oligosaccharide element, at present at home and abroad there is not yet relevant report.
Invention content
The purpose of the present invention is to propose to a kind of with synergistic function, use cost is low, preventive effect is good contains superfine chain lattice
The bactericidal composition of spore activator protein.
The present invention is achieved through the following technical solutions：
A kind of bactericidal composition of the activator protein containing toVerticilliumdahliaActivitie Activitie S of Relative, containing active components A and active constituent B, activity at
Point A is 1: 500~500: 1 with active constituent B weight ratios, and the active components A is toVerticilliumdahliaActivitie Activitie S of Relative activator protein, activity at
B is divided to be selected from one of chitosan, Portugal's polyene sugar, lentinan, oligosaccharide element kind；Preferably active components A and active constituent B
Weight ratio be 1 ︰, 100~100 ︰ 1；More preferably the weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and chitosan is 1: 50~50
: 1, the weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Portugal's polyene sugar is 1: 30~50: 1, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and mushroom
The weight ratio of polysaccharide is 1: 50~50: 1, and the weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and oligosaccharide element is 1: 50~50: 1；Most
Preferably the weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and chitosan is 1: 20~20: 1, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Portugal
The weight ratio of polyene sugar is 1: 1~30: 1, and the weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and lentinan is 1: 20~20: 1, pole
The weight ratio of alternaria tenuis activator protein and oligosaccharide element is 1: 20~20: 1.
Wettable powder, water-dispersible grain is made in the bactericidal composition of the activator protein containing toVerticilliumdahliaActivitie Activitie S of Relative, composition
Agent, suspending agent, suspension emulsion, aqueous emulsion, microemulsion, microcapsule suspending agent, microcapsule suspension-suspendinagent agent or aqua.
The bactericidal composition of the activator protein containing toVerticilliumdahliaActivitie Activitie S of Relative is used to prevent the disease of crop and promotes plant life
Long purposes；The crop includes legume crop, fibre crops, sugar crop, melon crop, fruits crop, dry fruit
Crop, hobby crop, root crop, oil crops, flower crop, medicinal crop, crop, green manure pasture crop；Institute
The disease stated includes virosis, powdery mildew, downy mildew, epidemic disease, banded sclerotial blight, head blight, stripe virus disease.
The amount of application when content of active constituent depends on being used alone in the bactericidal composition of the present invention, also depends on one
The mixed ratio and synergistic effect degree of kind compound and another compound, at the same it is also related with target disease.Usual group
The weight percentage for closing active constituent in object is the 1%~90% of total weight, preferably 5%~80%.According to different
Preparation type, active component content range are different.In general, liquid preparation contains by weight 1%~60% active matter
Matter, preferably 5%~50%；Solid pharmaceutical preparation contains by weight 5%~80% active material, preferably 10%~
80%.
The present invention bactericidal composition at least contain a kind of surfactant, in favor of application when active component in water
Dispersion.Surface-active contents are the 2%~30% of total formulation weight, and surplus is solid or liquid diluent.
The bactericidal composition of the present invention can used before through dilution or directly by user.Its preparation can be by ability
Prepared by the processing method well known to field technique personnel, i.e., after mixing active constituent with liquid flux or solid carrier, add
One or more of surfactant such as dispersant, stabilizer, wetting agent, binder, disintegrant, antifoaming agent etc..According to not
Same dosage form can also contain antifreeze known in those skilled in the art etc. in preparation.
Composition includes following component and content when wettable powder is made：Active components A 0.001%~60%, activity
Ingredient B 0.001%~80%, dispersant 1%~12%, wetting agent 1%~8%, filler surplus.
Composition includes following component and content when water dispersible granules are made：Active components A 0.001%~60%, activity
Ingredient B 0.001%~80%, dispersant 1%~12%, wetting agent 1%~8%, disintegrant 1%~10%, binder 0~
8%, filler surplus.
Composition includes following component and content when suspending agent is made：Active components A 0.001%~50%, active constituent
B0.001%~50%, dispersant 1%~10%, wetting agent 1%~10%, antifoaming agent 0.01%~2%, thickener 0~
2%, antifreeze 0~8%, deionized water add to 100%.
Composition includes following component and content when aqueous emulsion is made：Active components A 0.001%~50%, active constituent
B 0.001%~50%, solvent 1%~20%, emulsifier 1%~12%, antifreeze 0~8%, antifoaming agent 0.01%~2%,
Thickener 0~2%, deionized water add to 100%.
Composition includes following component and content when microemulsion is made：Active components A 0.001%~50%, active constituent B
0.001%~50%, emulsifier 3%~25%, solvent 1%~10%, antifreeze 0~8%, antifoaming agent 0.01%~2%, go
Ionized water adds to 100%.
Composition includes following component and content when suspension emulsion is made：Active components A 0.001%~50%, active constituent
B 0.001%~50%, emulsifier 1%~10%, dispersant 1%~10%, solvent 1%~20%, antifoaming agent 0.01%~
2%, thickener 0~2%, antifreeze 0~8%, deionized water add to 100%.
Composition includes following component and content when microcapsule suspending agent is made：Active components A 0.001%~50%, activity
Ingredient B 0.001%~50%, macromolecule cyst material 1%~10%, dispersant 2%~10%, solvent 1%~10%, breast
Agent 1%~7%, pH adjusting agent 0.01%~5%, antifoaming agent 0.01%~2%, deionized water add to 100%.
Composition includes following component and content when microcapsule suspension-suspendinagent agent is made：Active components A 0.001%~50%,
Active constituent B 0.001%~50%, macromolecule cyst material 1%~12%, dispersant 1%~12%, wetting agent 1%~
8%, solvent 1%~15%, emulsifier 1%~8%, antifoaming agent 0.01%~2%, thickener 0~2%, pH adjusting agent
0.01%~5%, deionized water adds to 100%.
Composition includes following constituent content when aqua is made：Active components A 0.001%~40%, active constituent B
0.001%~40%, cosolvent 2%~6%, wetting agent 1%-10%, antifreeze 0~8%, deionized water add to 100%.This
The wettable powder the key technical indexes of invention：
The wettable powder the key technical indexes of the present invention：
The water dispersible granules the key technical indexes of the present invention：
The suspending agent the key technical indexes of the present invention：
The aqueous emulsion the key technical indexes of the present invention：
The microemulsion the key technical indexes of the present invention：
The suspension emulsion the key technical indexes of the present invention：
The microcapsule suspending agent the key technical indexes of the present invention：
The microcapsule suspension-suspendinagent agent the key technical indexes of the present invention：
The aqua the key technical indexes of the present invention：
The advantage of the invention is that：
(1) present composition has good synergy and lasting effect, preventive effect to be higher than single dose in a certain range；(2) agriculture
Medicinal dose is reduced, and is reduced residual quantity of the pesticide on crop, is mitigated environmental pollution；(3) fungicidal spectrum is expanded, to multiple diseases
Such as virosis, powdery mildew, downy mildew, epidemic disease, banded sclerotial blight, head blight, stripe virus disease have greater activity.
Specific implementation mode
With reference to embodiment to further instruction of the present invention, the percentage in embodiment is weight percentage, but
The present invention is not limited thereto.
Application Example one
1~15 wettable powder of embodiment
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein, active constituent B, dispersant, wetting agent, filler are mixed, mixed in mixed cylinder
Uniformly, it remixes after being smashed by air jet mill uniformly, you can wettable powder product of the present invention is made.Specifically it is shown in Table
1。
1 embodiment of table, 1~15 each component and content
16~30 water dispersible granules of embodiment
By toVerticilliumdahliaActivitie Activitie S of Relative activator protein, active constituent B, dispersant, wetting agent, disintegrant, filler etc. together through air-flow
The grain size needed is crushed, other auxiliary agents such as binder (can add can be not added with) are added, obtains granulation materials.Item is quantified
It is sent into fluidized bed granulating dryer after granulation and drying, you can water dispersible granules product of the present invention, tool is made
Body is shown in Table 2.
2 embodiment of table, 16~30 each component and content
Chitosan, Portugal's polyene sugar, lentinan, oligosaccharide element in table 1~2 are exchanged, novel formulation can be made.
31~41 suspending agent of embodiment
By dispersant, wetting agent, antifoaming agent, thickener (can add can be not added with), antifreeze (can add can be not added with), by high speed
Shear-mixed is uniform, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, active constituent B is added, ball milling 2~3 hours, make particle grain in the ball mill
Diameter is all at 5 μm hereinafter, surplus is supplied with deionized water, you can suspending agent product of the present invention is made, is specifically shown in Table 3.
3 embodiment of table, 31~41 each component and content
42~49 aqueous emulsion of embodiment
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein, active constituent B, solvent, emulsifier are added together, make to be dissolved into homogeneous oil phase；
Deionized water, antifreeze (can add can be not added with), thickener (can add can be not added with), antifoaming agent are mixed, at uniform water phase.
Under high velocity agitation, oil phase is added in water phase, aqueous emulsion product of the present invention is made.Specifically it is shown in Table 4.
4 embodiment of table, 42~49 each component and content
50~57 microemulsion of embodiment
By toVerticilliumdahliaActivitie Activitie S of Relative activator protein, active constituent B solutions in the homogenizer equipped with solvent, by emulsifier, antifreeze
(can add can be not added with), antifoaming agent are added in the homogenizer equipped with above-mentioned solution, and surplus gives strongly after being supplied with deionized water
It mixes and homogenizes, finally obtain the transparent microemulsion product of the present invention of clear appearance.Specifically it is shown in Table 5.
5 embodiment of table, 50~57 each component and content
Chitosan, Portugal's polyene sugar, lentinan in table 3~5 are exchanged, novel formulation can be made.
58~61 suspension emulsion of embodiment
Dispersant, antifoaming agent, thickener (can add can be not added with), antifreeze (can add can be not added with) are mixed by high speed shear
Uniformly, be added active constituent B, ball milling 2~3 hours in the ball mill, make diameter of particle all at 5 μm hereinafter, be made it is active at
Divide B suspending agents, then directly emulsifies toVerticilliumdahliaActivitie Activitie S of Relative activator protein, emulsifier, solvent and various auxiliary agents with high speed agitator
Into suspending agent, suspension emulsion product of the present invention is made.Specifically it is shown in Table 6.
6 embodiment of table, 58~61 each component and content
62~65 microcapsule suspending agent of embodiment
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein, active constituent B, macromolecule cyst material, solvent are mixed, make to be dissolved into uniform oil
Phase, in a shear condition, by oil phase be added to containing emulsifier, pH adjusting agent, dispersant, antifoaming agent aqueous phase solution in, it is remaining
Amount is supplied with deionized water, and two kinds of materials react in oil-water interfaces, forms high score ascus wall, and the present composition point is made
Dissipate good microcapsule suspending agent product.Specifically it is shown in Table 7.
7 embodiment of table, 62~65 each component and content
66~69 microcapsule suspension-suspendinagent agent of embodiment
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein, macromolecule cyst material, solvent are mixed, make to be dissolved into homogeneous oil phase, by oil phase
Be added in a shear condition containing emulsifier, pH adjusting agent aqueous phase solution in, well dispersed microcapsule suspending agent is made.It will
Dispersant, wetting agent, antifoaming agent, thickener (can add can be not added with) are uniformly mixed by high speed shear, and active constituent B is added,
Ball milling 2~3 hours in ball mill make diameter of particle all at 5 μm hereinafter, suspending agent is made, are then added to suspending agent micro-
In the aqueous phase solution of capsule suspension, deionized water supplies surplus, and it is outstanding that the well dispersed microcapsule suspension-of the present composition is made
Floating agent product.Specifically it is shown in Table 8.
8 embodiment of table, 66~69 each component and content
70~85 aqua of example
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein, active constituent B, wetting agent, antifreeze, solvent, deionized water etc. are mixed together,
The aqua product of the present composition is made, is specifically shown in Table 9.
9 example of table, 67~85 component and content
Chitosan, Portugal's polyene sugar, lentinan, oligosaccharide element in table 9 are exchanged, novel formulation can be made.
The embodiment of the present invention is the method being combined using Toxicity Determination and field trial.First pass through indoor virulence survey
It is fixed, the synergy ratio (SR) after two kinds of medicaments compound by a certain percentage is defined, SR ＜ 0.5 are antagonism, 0.5≤SR≤1.5
For summation action, SR ＞ 1.5 are synergistic effect, on this basis, then carry out field trial.
Test method：After prerun determines the effective inhibition concentration range of each medicament, medicament sets 5 respectively by active constituent content
A dosage processing, if clear water compares.Reference《Farm-chemical indoor determination test rule fungicide》It carries out, speed is grown using mycelia
Rate method measures virulence of the medicament to crop germ.Colony diameter is measured with crossing method after 72h, calculates the net growth of each processing
Amount, mycelial growth inhibition rate.
Net growth (mm)=measurement colony diameter -5
Mycelial growth inhibition rate is converted into probit value (y), liquor strength (μ g/mL) is converted into logarithm (x), with minimum
Square law acquires virulence regression equation (y=a+bx), and thus calculates the EC of each medicament50Value.Simultaneously according to Wadley methods
Two medicament different ratio synergy ratios (SR) are calculated, SR ＜ 0.5 are antagonism, and 0.5≤SR≤1.5 are summation action,
SR ＞ 1.5 are synergistic effect.Calculation formula is as follows：
Wherein：A, b is respectively active components A and active constituent B ratio shared in combination；
A is toVerticilliumdahliaActivitie Activitie S of Relative activator protein；
B is selected from one of chitosan, Portugal's polyene sugar, lentinan, oligosaccharide element kind.
Application Example two：
For trying disease：Tomato blight
Test medicine is provided by Shanxi Thompson Biological Technology Co., Ltd..
Experimental design：Both toVerticilliumdahliaActivitie Activitie S of Relative activator protein and chitosan different ratio mixture are determined by pilot study
Effective inhibition concentration range.
Toxicity test result
10 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table compounds the toxicity test analysis of results table to tomato blight with chitosan
As shown in Table 10, toVerticilliumdahliaActivitie Activitie S of Relative activator protein prevents the proportioning of tomato blight in 1 ︰ 500 with chitosan compounding
When~500 ︰ 1, synergy ratio SR is all higher than 1.5, illustrates that the two mixture in 1 ︰, 500~500 ︰, 1 ranges shows synergy work
With the proportioning of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and chitosan is 1: 50~50: 1, and synergistic effect is more prominent, and synergy ratio is equal
2.20 or more, wherein toVerticilliumdahliaActivitie Activitie S of Relative activator protein and chitosan weight ratio are 2：Synergy ratio is maximum when 3, synergistic effect
It is the most apparent.Tested through applicant find the proportioning of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and chitosan be 1 ︰ 50,1 ︰ 40,1 ︰ 30,
1 ︰ 25,1 ︰ 20,1 ︰ 15,1 ︰ 10,1 ︰ 9,1 ︰ 8,1 ︰ 7,1 ︰ 6,1 ︰ 5,1 ︰ 4,1 ︰ 3,1 ︰ 2,1 ︰ 1,3 ︰ 2,2 ︰ 1,3 ︰ 1,4 ︰ 1,5 ︰ 1,
When 6 ︰ 1,7 ︰ 1,8 ︰ 1,9 ︰ 1,10 ︰ 1,15 ︰ 1,20 ︰ 1,25 ︰ 1,30 ︰ 1,40 ︰ 1,50 ︰ 1, to virosis, powdery mildew, downy mildew,
The prevention of epidemic disease, banded sclerotial blight, head blight, stripe virus disease has apparent synergistic effect, and synergy ratio is 1.50 or more.
Application Example three：
For trying disease：Tomato virus disease
Test medicine is provided by Shanxi Thompson Biological Technology Co., Ltd..
Experimental design：Both toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Portugal's polyene sugar different ratio mixture are determined by pilot study
Effective inhibition concentration range.
Toxicity test result
11 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table is with Portugal's polyene sugar compounding to the toxicity test analysis of results table of tomato virus disease
As shown in Table 11, toVerticilliumdahliaActivitie Activitie S of Relative activator protein is matched with Portugal's polyene sugar compounding prevention tomato virus disease in 1 ︰ 500
When~500 ︰ 1, synergy ratio SR is all higher than 1.5, illustrates that the two mixture in 1 ︰, 50~50 ︰, 1 ranges shows synergistic effect,
The proportioning of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Portugal's polyene sugar is 1: 30~50: 1, and synergistic effect is more prominent, and synergy ratio exists
2.20 or more, wherein toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Portugal's polyene sugar weight ratio are 14：Synergy ratio is maximum when 1, synergistic effect
It is the most apparent.Tested through applicant find the proportioning of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Portugal's polyene sugar be 1 ︰ 30,1 ︰ 25,1 ︰ 20,
1 ︰ 15,1 ︰ 10,1 ︰ 5,1 ︰ 1,4 ︰ 1,5 ︰ 1,6 ︰ 1,7 ︰ 1,8 ︰ 1,9 ︰ 1,10 ︰ 1,11 ︰ 1,12 ︰ 1,13 ︰ 1,14 ︰ 1,15 ︰ 1,16 ︰ 1,
When 17 ︰ 1,18 ︰ 1,19 ︰ 1,20 ︰ 1,21 ︰ 1,22 ︰ 1,23 ︰ 1,24 ︰ 1,25 ︰ 1,29 ︰ 1,35 ︰ 1,40 ︰ 1,45 ︰ 1,50 ︰ 1, to disease
The prevention of viral disease, powdery mildew, downy mildew, epidemic disease, banded sclerotial blight, head blight, stripe virus disease has apparent synergistic effect, synergy
Ratio is 1.50 or more.
Application Example four：
For trying disease：Tomato virus disease
Test medicine is provided by Shanxi Thompson Biological Technology Co., Ltd..
Experimental design：Both toVerticilliumdahliaActivitie Activitie S of Relative activator protein and lentinan different ratio mixture are determined by pilot study
Effective inhibition concentration range.
Toxicity test result
12 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table compounds the toxicity test analysis of results table to tomato virus disease with lentinan
As shown in Table 12, toVerticilliumdahliaActivitie Activitie S of Relative activator protein prevents the proportioning of tomato virus disease in 1 ︰ with lentinan compounding
When 500~500 ︰ 1, synergy ratio SR is all higher than 1.5, illustrates that the two mixture in 1 ︰, 500~500 ︰, 1 ranges shows synergy
The proportioning of effect, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and lentinan is 1: 50~50: 1, and synergistic effect is more prominent, synergy ratio
2.20 or more, wherein toVerticilliumdahliaActivitie Activitie S of Relative activator protein and lentinan weight ratio are 1：Synergy ratio is maximum when 1, and synergy is made
With the most obviously.It is tested through applicant and finds that the proportioning of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and lentinan is 1 ︰ 50,1 ︰ 40,1 ︰
30,1 ︰ 25,1 ︰ 20,1 ︰ 15,1 ︰ 10,1 ︰ 9,1 ︰ 8,1 ︰ 7,1 ︰ 6,1 ︰ 5,1 ︰ 4,1 ︰ 3,1 ︰ 2,1 ︰ 1,3 ︰ 2,2 ︰ 1,3 ︰ 1,4 ︰ 1,5 ︰
1, when 6 ︰ 1,7 ︰ 1,8 ︰ 1,9 ︰ 1,10 ︰ 1,15 ︰ 1,20 ︰ 1,25 ︰ 1,30 ︰ 1,40 ︰ 1,50 ︰ 1, to virosis, powdery mildew, downy mildew
The prevention of disease, epidemic disease, banded sclerotial blight, head blight, stripe virus disease has an apparent synergistic effect, synergy ratio 1.50 with
On.
Application Example five：
For trying disease：Rice sheath blight disease
Test medicine is provided by Shanxi Thompson Biological Technology Co., Ltd..
Experimental design：Both toVerticilliumdahliaActivitie Activitie S of Relative activator protein and oligosaccharide element different ratio mixture are determined by pilot study
Effective inhibition concentration range.
Toxicity test result
13 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table compounds the toxicity test analysis of results table to rice sheath blight disease with oligosaccharide element
As shown in Table 13, toVerticilliumdahliaActivitie Activitie S of Relative activator protein prevents the proportioning of rice sheath blight disease in 1 ︰ with oligosaccharide element compounding
When 500~500 ︰ 1, synergy ratio SR is all higher than 1.5, illustrates that the two mixture in 1 ︰, 500~500 ︰, 1 ranges shows synergy
The proportioning of effect, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and oligosaccharide element is 1: 50~50: 1, and synergistic effect is more prominent, synergy ratio
2.15 or more, wherein toVerticilliumdahliaActivitie Activitie S of Relative activator protein and oligosaccharide element weight ratio are 3：Synergy ratio is maximum when 2, and synergy is made
With the most obviously.It is tested through applicant and finds that the proportioning of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and oligosaccharide element is 1 ︰ 50,1 ︰ 40,1 ︰
30,1 ︰ 25,1 ︰ 20,1 ︰ 15,1 ︰ 10,1 ︰ 9,1 ︰ 8,1 ︰ 7,1 ︰ 6,1 ︰ 5,1 ︰ 4,1 ︰ 3,1 ︰ 2,1 ︰ 1,3 ︰ 2,2 ︰ 1,3 ︰ 1,4 ︰ 1,5 ︰
1, when 6 ︰ 1,7 ︰ 1,8 ︰ 1,9 ︰ 1,10 ︰ 1,15 ︰ 1,20 ︰ 1,25 ︰ 1,30 ︰ 1,40 ︰ 1,50 ︰ 1, to virosis, powdery mildew, downy mildew
The prevention of disease, epidemic disease, banded sclerotial blight, head blight, stripe virus disease has an apparent synergistic effect, synergy ratio 1.50 with
On.
Application Example six：
For studying object：Tomato
Test medicine is provided by Shanxi Thompson Biological Technology Co., Ltd..
Experimental design：Determine that toVerticilliumdahliaActivitie Activitie S of Relative activator protein is mixed with active constituent B both different ratio by pilot study
The concentration range of agent coordinate plant growth.
14 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table grows the adjusting of tomato with the mixed liquid of active constituent B and its different proportion
Effect
As shown in Table 14, after toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active constituent B are used in mixed way, can activated plant metabolism system
It unites and plant growth is made to accelerate vigor enhancing, the increase of proline content and the reinforcement of cellulase, plant cell is promoted to increase,
Root activity is reinforced, and volume increase purpose is reached, and toVerticilliumdahliaActivitie Activitie S of Relative activator protein, in 1 500~500 ︰ 1 of ︰, has with active constituent B
Stronger adjustment effect.Especially when toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active constituent B proportionings are in 1 ︰ 50,1 ︰ 40,1 ︰ 30,1 ︰
25,1 ︰ 20,1 ︰ 15,1 ︰ 10,1 ︰ 9,1 ︰ 8,1 ︰ 7,1 ︰ 6,1 ︰ 5,1 ︰ 4,1 ︰ 3,1 ︰ 2,1 ︰ 1,3 ︰ 2,2 ︰ 1,3 ︰ 1,4 ︰ 1,5 ︰ 1,6 ︰
1, when 7 ︰ 1,8 ︰ 1,9 ︰ 1,10 ︰ 1,15 ︰ 1,20 ︰ 1,25 ︰ 1,30 ︰ 1,40 ︰ 1,50 ︰ 1, adjustment effect becomes apparent protrusion.
Effect experiment part：Test medicine is researched and developed by Shanxi Thompson Biological Technology Co., Ltd., is provided, comparison medicament
3% toVerticilliumdahliaActivitie Activitie S of Relative activator protein wettable powder (purchased in market), 2% chitosan aqua (purchased in market), 0.5% Portugal's polyene sugar are wettable
Property pulvis (purchased in market), 1% lentinan aqua (purchased in market), 6% oligosaccharide element aqua (purchased in market).
Seven toVerticilliumdahliaActivitie Activitie S of Relative activator protein of Application Example and active constituent B and its compounding prevention tomato blight drug effect and
Growth is adjusted to be tested
This experiment is arranged in Xi'an City, Shanxi Province, investigates the tomato blight state of an illness before medicine, in state of an illness first time at initial stage dispenser, often
Dispenser in 7 days is primary, altogether dispenser 2 times.3 days after second of dispenser, disease index is investigated respectively and calculates preventive effect within 7 days, 15 days.Experiment
As a result as follows：
15 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table is made with active constituent B and its compounding prevention tomato blight drug effect and adjusting growth
Experiment
As can be seen from Table 15, tomato can effectively be prevented after toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active constituent B and its compounding
Epidemic disease, control effect are superior to the preventive effect of single dose, and preventive effect phase is long, and can activated plant metabolic system and so that plant growth is accelerated
Vigor enhances, and enhances the growth of root system.It has no adverse effects simultaneously to target crop in experiment scope of medication.
Application Example octupole alternaria tenuis activator protein and active constituent B and its compounding prevention cucumber downy mildew drug effect
And it adjusts growth and is tested
This test arrangement investigates the cucumber downy mildew state of an illness before Shaanxi Province Jingyang County, medicine, in state of an illness first time at initial stage dispenser,
Every dispenser in 7 days is primary, altogether dispenser 2 times.3 days after second of dispenser, disease index is investigated respectively and calculates preventive effect within 7 days, 15 days.It is real
It is as follows to test result：
16 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table is grown with active constituent B and its compounding prevention cucumber downy mildew drug effect and adjusting
It is tested
As can be seen from Table 16, cucumber can effectively be prevented after toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active constituent B and its compounding
Downy mildew, control effect are superior to the preventive effect of single dose, and preventive effect phase is long, and can activated plant metabolic system and so that plant growth is added
Fast vigor enhancing, enhances the growth of root system.It has no adverse effects simultaneously to target crop in experiment scope of medication.
Nine toVerticilliumdahliaActivitie Activitie S of Relative activator protein of Application Example and active constituent B and its compounding prevention tomato virus disease drug effect
And it adjusts growth and is tested
This experiment is arranged in Xi'an City, Shanxi Province, and the tomato virus disease state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser,
Every dispenser in 7 days is primary, altogether dispenser 2 times.7 days after second of dispenser, disease index is investigated respectively and calculates preventive effect within 14 days, 30 days.
Experimental result is as follows：
17 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table is grown with active constituent B and its compounding prevention tomato virus disease drug effect and adjusting
Experiment on Function
As can be seen from Table 17, tomato can effectively be prevented after toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active constituent B and its compounding
Virosis, control effect are superior to the preventive effect of single dose, and preventive effect phase is long, and can activated plant metabolic system and so that plant growth is added
Fast vigor enhancing, enhances the growth of root system.It has no adverse effects simultaneously to target crop in experiment scope of medication.
Ten toVerticilliumdahliaActivitie Activitie S of Relative activator protein of Application Example and active constituent B and its compounding prevention rice sheath blight disease drug effect
And it adjusts growth and is tested
This experiment is arranged in Shaanxi Province's Hanzhong City, and the rice sheath blight disease state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser,
Every dispenser in 7 days is primary, altogether dispenser 2 times.3 days after second of dispenser, disease index is investigated respectively and calculates preventive effect within 7 days, 15 days.It is real
It is as follows to test result：
18 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table is grown with active constituent B and its compounding prevention rice sheath blight disease drug effect and adjusting
Experiment on Function
As can be seen from Table 18, rice can effectively be prevented after toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active constituent B and its compounding
Banded sclerotial blight, control effect are superior to the preventive effect of single dose, and preventive effect phase is long, and can activated plant metabolic system and so that plant growth is added
Fast vigor enhancing, enhances the growth of root system.It has no adverse effects simultaneously to target crop in experiment scope of medication.
11 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of Application Example and active constituent B and its compounding prevention rice stripe leaf are withered
Sick drug effect and adjusting growth are tested
This experiment is arranged in Shaanxi Province's Hanzhong City, and the stripe disease state of an illness is investigated before medicine, in first time at state of an illness initial stage
Dispenser, every dispenser in 7 days is primary, altogether dispenser 2 times.7 days after second of dispenser, disease index is investigated respectively and is calculated within 14 days, 30 days
Preventive effect.Experimental result is as follows：
19 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table and active constituent B and its compounding prevention stripe disease drug effect and adjusting
Growth is tested
As can be seen from Table 19, rice can effectively be prevented after toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active constituent B and its compounding
Stripe virus disease, control effect are superior to the preventive effect of single dose, and preventive effect phase is long, and can activated plant metabolic system and so that plant is given birth to
Long quickening vigor enhancing, enhances the growth of root system.It has no adverse effects simultaneously to target crop in experiment scope of medication.
12 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of Application Example and active constituent B and its compounding prevention powdery mildew of cucumber medicine
Effect and adjusting growth are tested
This experiment is arranged in Shaanxi Province Jingyang County, and the powdery mildew of cucumber state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser,
Every dispenser in 7 days is primary, altogether dispenser 2 times.3 days after second of dispenser, disease index is investigated respectively and calculates preventive effect within 7 days, 15 days.It is real
It is as follows to test result：
20 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table is grown with active constituent B and its compounding prevention powdery mildew of cucumber drug effect and adjusting
Experiment on Function
As can be seen from Table 20, cucumber can effectively be prevented after toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active constituent B and its compounding
Powdery mildew, control effect are superior to the preventive effect of single dose, and preventive effect phase is long, and can activated plant metabolic system and so that plant growth is added
Fast vigor enhancing, enhances the growth of root system.It has no adverse effects simultaneously to target crop in experiment scope of medication.
13 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of Application Example and active constituent B and its compounding prevention tomato virus disease medicine
Effect and adjusting growth are tested
This experiment is arranged in Xi'an City, Shanxi Province, and the tomato virus disease state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser,
Every dispenser in 7 days is primary, altogether dispenser 2 times.3 days after second of dispenser, disease index is investigated respectively and calculates preventive effect within 7 days, 15 days.It is real
It is as follows to test result：
21 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table is grown with active constituent B and its compounding prevention tomato virus disease drug effect and adjusting
Experiment on Function
As can be seen from Table 21, tomato can effectively be prevented after toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active constituent B and its compounding
Virosis, control effect are superior to the preventive effect of single dose, and preventive effect phase is long, and can activated plant metabolic system and so that plant growth is added
Fast vigor enhancing, enhances the growth of root system.It has no adverse effects simultaneously to target crop in experiment scope of medication.
The experiment by different places throughout the country obtains afterwards, and toVerticilliumdahliaActivitie Activitie S of Relative activator protein is compounded with active constituent B
Afterwards to Common Diseases such as virosis, powdery mildew, downy mildew, epidemic disease, banded sclerotial blight, head blight, stripe virus diseases in various crop
Preventive effect 95% on excellent, in single dose preventive effect, synergistic effect is apparent.Simultaneously can activated plant metabolic system and make plant
The enhancing of quickening vigor, the increase of proline content and the reinforcement of cellulase are grown, plant cell is promoted to increase, root activity adds
By force, reach volume increase purpose.
Claims (6)
1. a kind of bactericidal composition of activator protein containing toVerticilliumdahliaActivitie Activitie S of Relative, it is characterised in that：Effective active composition is effective active
Ingredient A and B, active components A are 1: 500~500: 1 with active constituent B weight ratios, and the active components A is toVerticilliumdahliaActivitie Activitie S of Relative
Activator protein, active constituent B are chitosan.
2. the bactericidal composition of the activator protein according to claim 1 containing toVerticilliumdahliaActivitie Activitie S of Relative, it is characterised in that：Active constituent
The weight ratio of A and active constituent B is 1: 100~100: 1.
3. the bactericidal composition of the activator protein according to claim 2 containing toVerticilliumdahliaActivitie Activitie S of Relative, it is characterised in that：
The weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and chitosan is 1: 50~50: 1.
4. the bactericidal composition of the activator protein described in any one of claim 1 to 3 containing toVerticilliumdahliaActivitie Activitie S of Relative, feature exist
In：Wettable powder, water dispersible granules, suspending agent, suspension emulsion, aqueous emulsion, microemulsion, microcapsule suspending agent, micro- is made in composition
Capsule suspension-suspending agent or aqua.
5. the bactericidal composition of the activator protein according to claim 4 containing toVerticilliumdahliaActivitie Activitie S of Relative is for preventing crop disease and rush
Into the purposes of plant growth.
6. purposes according to claim 5, it is characterised in that：The disease is virosis, powdery mildew, downy mildew, epidemic disease
Disease, banded sclerotial blight, head blight or stripe virus disease.
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| CN107549199A (en) * | 2017-10-06 | 2018-01-09 | 湖南泽丰农化有限公司 | Efficient bactericidal composite containing lentinan and toVerticilliumdahliaActivitie Activitie S of Relative activator protein |
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| CN101385470A (en) * | 2008-10-24 | 2009-03-18 | 吉林省农业科学院 | Novel environmental protection compound pesticide for preventing and treating rice blast |
| CN102687730A (en) * | 2011-03-25 | 2012-09-26 | 中国农业科学院植物保护研究所 | Biological compound preparation for controlling plant virus disease and application thereof |
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| CN101341885A (en) * | 2008-08-29 | 2009-01-14 | 中国农业科学院植物保护研究所 | Preparation and uses of plant activation protein controllable sustained-release nano-microsphere |
| CN101385470A (en) * | 2008-10-24 | 2009-03-18 | 吉林省农业科学院 | Novel environmental protection compound pesticide for preventing and treating rice blast |
| CN102687730A (en) * | 2011-03-25 | 2012-09-26 | 中国农业科学院植物保护研究所 | Biological compound preparation for controlling plant virus disease and application thereof |
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