CN107041382A - A kind of efficient bactericidal composite containing bacillus amyloliquefaciens - Google Patents
A kind of efficient bactericidal composite containing bacillus amyloliquefaciens Download PDFInfo
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- CN107041382A CN107041382A CN201710255216.6A CN201710255216A CN107041382A CN 107041382 A CN107041382 A CN 107041382A CN 201710255216 A CN201710255216 A CN 201710255216A CN 107041382 A CN107041382 A CN 107041382A
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- bacillus amyloliquefaciens
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Abstract
The invention discloses a kind of efficient bactericidal composite containing bacillus amyloliquefaciens, bactericidal composition containing active component bacillus amyloliquefaciens Yu active component B, and the weight range of bacillus amyloliquefaciens be 2 700 hundred million gemma/gram, active component B weight range is 0.01~50%.The present composition has greater activity to the multiple diseases in various crop, and with obvious synergistic effect, expands fungicidal spectrum.And with dosage is small, resistance of rainwater washing against, the characteristics of synergy is obvious.
Description
Technical field
The invention belongs to technical field of pesticide, it is related to a kind of efficient bactericidal composite containing bacillus amyloliquefaciens in crop
Application in disease.
Technical background
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), the bacillus amyloliquefaciens mechanism of action is main
Including secretion antibacterial material, antagonism, nutrition and the competition in space are produced, induction host produces resistance and promotes plant growth
Deng.
In the real process of agricultural production, controlling disease is easiest to generate the problem that the generation of disease drug resistance.No
Compounded with kind composition, be to prevent and treat resistance disease very common method.Heterogeneity is compounded, and is imitated according to practical application
Really, come judge certain compounding be synergy, plus and or antagonism.In most cases, the compounding effect of agricultural chemicals is all to add
And effect, really have the compounding of synergistic effect seldom, especially synergistic effect clearly, synergy ratio very high compounding just more
It is few.Studied by inventor, discovery mutually compounds bacillus amyloliquefaciens with active component B, has within the specific limits very
Good synergistic effect, and about bacillus amyloliquefaciens compounding related to active component B's, at present at home and abroad there is not yet related
Report.
The content of the invention
The purpose of the present invention is to propose to a kind of with synergistic function, the starch bud containing solution that use cost is low, preventive effect is good
The efficient bactericidal composite of spore bacillus.
The present invention is to be achieved through the following technical solutions：
A kind of efficient bactericidal composite containing bacillus amyloliquefaciens, contains bacillus amyloliquefaciens and active component B, solution
The weight range of bacillus amyloliquefacienses be the gemma of 2-700 hundred million/gram, active component B weight range is 0.01~50%；Solve starch
The weight range of bacillus be the gemma of 5-500 hundred million/gram, active component B weight range is 0.05~30%；More preferably solve
The weight range of bacillus amyloliquefacienses be the gemma of 7-400 hundred million/gram, active component B weight range is 0.1~20%, activity into
It is polyoxin, kasugarnycin, Ningnanmycin, Zhongshengmycin, antibiotic in pyrimidine nucleoside class, antibiotic in pyrimidine nucleoside class to divide B
It is pesticide corrosion 120.
The described bactericidal composition containing bacillus amyloliquefaciens is used for the application for preventing and treating the disease of crop, described crop
Make including cereal crops, legume crop, fibre crops, sugar crop, melon crop, fruits crop, dry fruit crop, hobby
Thing, root crop, oil crops, flower crop, medicinal crop, crop, green manure pasture crop.
The described bactericidal composition containing bacillus amyloliquefaciens is used for the application for preventing and treating the disease of crop, described disease
Including rice blast, anthracnose of peach, Pear black spot, apple brown rot, capsicum black rot, early blight, damping-off, root rot,
Downy mildew, spot defoliation, epidemic disease, gray mold, powdery mildew, virosis.
The amount of application when content of active component depends on being used alone in the present composition, also depends on a kind of chemical combination
Thing and the mixed ratio and synergistic effect degree of another compound, while also relevant with target disease.In usual composition
The weight percentage of active component is the 1%~90% of gross weight, preferably 5%~80%.According to different preparation classes
Type, active component content scope is different.Generally, liquid preparation contains by weight 1%~60% active material, preferably
Ground is 5%~50%；Solid pharmaceutical preparation contains by weight 5%~80% active material, preferably 10%~80%.
At least contain a kind of surfactant in the bactericidal composition of the present invention, active component is in water during in favor of applying
It is scattered.Surface-active contents are the 2%~30% of total formulation weight, and surplus is solid or liquid diluent.
Surfactant selected by the bactericidal composition of the present invention is known in those skilled in the art：It can be selected from
One or more in dispersant, wetting agent, thickener or defoamer.According to different dosage forms, this area can also be contained in preparation
Stabilizer, antifreeze well known to technical staff etc..
The present invention bactericidal composition can by user using it is preceding through dilute or directly use.Its preparation can be by ability
Prepared by the processing method well known to field technique personnel, i.e., after active component is mixed with liquid flux or solid carrier, add
One or more in surfactant such as dispersant, stabilizer, wetting agent, binding agent, defoamer.
The bactericidal composition of the present invention, acceptable formulation on any agricultural chemicals can be processed on demand.Wherein preferred agent
Type is wettable powder, water dispersible granules, suspending agent, suspension emulsion, microemulsion, aqueous emulsion, aqua.
Composition includes following component and content when wettable powder is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/
Gram, active component B 0.01%~50%, dispersant 1%~12%, wetting agent 1%~8%, filler surplus.
Composition includes following component and content when water dispersible granules are made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/
Gram, active component B 0.01%~50%, dispersant 1%~12%, wetting agent 1%~8%, disintegrant 1%~10%, bond
Agent 0~8%, filler surplus.
Composition includes following component and content when suspending agent is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, it is living
Property composition B 0.01%~50%, dispersant 1%~10%, wetting agent 1%~10%, defoamer 0.01%~2%, thickener
0~2%, antifreeze 0~8%, deionized water add to 100%.
Composition includes following component and content when suspension emulsion is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, it is living
Property composition B 0.01%~50%, emulsifying agent 1%~10%, dispersant 1%~10%, solvent 1%~20%, defoamer
0.01%~2%, thickener 0~2%, antifreeze 0~8%, deionized water add to 100%.
Composition includes following constituent content when aqua is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, activity into
B 0.01%~50%, cosolvent 2%~6%, wetting agent 1%-10%, antifreeze 0~8%, deionized water is divided to add to
100%.
Composition includes following component and content when aqueous emulsion is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, it is living
Property composition B 0.01%~50%, solvent 1%~20%, emulsifying agent 1%~12%, antifreeze 0~8%, defoamer 0.01%
~2%, thickener 0~2%, deionized water add to 100%.
Composition includes following component and content when microemulsion is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, it is living
Property composition B 0.01%~50%, emulsifying agent 3%~25%, solvent 1%~10%, antifreeze 0~8%, defoamer 0.01%
~2%, deionized water adds to 100%.
The wettable powder the key technical indexes of the present invention：
The water dispersible granules the key technical indexes of the present invention：
The suspending agent the key technical indexes of the present invention：
The suspension emulsion the key technical indexes of the present invention：
The aqua the key technical indexes of the present invention：
The aqueous emulsion the key technical indexes of the present invention：
The microemulsion the key technical indexes of the present invention：
The advantage of the invention is that：
(1) present composition has good synergy and lasting effect within the specific limits, and preventive effect is higher than single dose；(2) agriculture
Medicinal dose is reduced, residual quantity of the reduction agricultural chemicals on crop, mitigates environmental pollution；(3) fungicidal spectrum is expanded, to multiple diseases
Such as rice blast, anthracnose of peach, Pear black spot, apple brown rot, capsicum black rot, early blight, damping-off, root rot, frost
Mildew, spot defoliation, epidemic disease, gray mold, powdery mildew, virosis have greater activity.
Embodiment
With reference to embodiment to further instruction of the present invention, the percentage in embodiment is weight percentage, but
The invention is not limited in this.
Application Example one
The wettable powder of embodiment 1~15
Bacillus amyloliquefaciens, active component B, dispersant, wetting agent, filler are mixed, are well mixed in mixed cylinder,
Remixed after being crushed through airslide disintegrating mill uniform, you can wettable powder product of the present invention is made.Specifically it is shown in Table 1.
The each component of 1 embodiment of table 1~15 and content
The water dispersible granules of embodiment 16~25
Bacillus amyloliquefaciens, active component B, dispersant, wetting agent, disintegrant, filler are obtained through air-flow crushing together
To the particle diameter of needs, other auxiliary agents such as binding agent (can add to be not added with) are added, materials of pelletizing are obtained.Item is quantified into feeding stream
Change in bed granulating drier after granulation and drying, water dispersible granules product of the present invention is made.Specifically it is shown in Table 2.
The each component of 2 embodiment of table 16~25 and content
The suspending agent of embodiment 26~35
By dispersant, wetting agent, thickener (can add to be not added with), defoamer, antifreeze (can add to be not added with) by a high speed
Shear-mixed is uniform, adds bacillus amyloliquefaciens, active component B, supplies surplus with deionized water, ball milling 2 in ball mill
~3 hours, make diameter of particle all below 5 μm, suspending agent product of the present invention is made.Specifically it is shown in Table 3.
The each component of 3 embodiment of table 26~35 and content
The suspension emulsion of embodiment 36~37
By dispersant, defoamer, thickener (can add to be not added with), antifreeze (can add to be not added with), deionized water through too high
Fast shear-mixed is uniform, adds bacillus amyloliquefaciens, ball milling 2~3 hours in ball mill make diameter of particle all at 5 μm
Hereinafter, bacillus amyloliquefaciens suspending agent is made, then by active component B, solvent, emulsifying agent and various auxiliary agent high-speed stirreds
Device is directly emulsified into suspending agent, and suspension emulsion product of the present invention is made.Specifically it is shown in Table 4.
The each component of 4 embodiment of table 36~37 and content
The aqueous emulsion of embodiment 38~47
Bacillus amyloliquefaciens, active component B, solvent, emulsifying agent are added together, make to be dissolved into homogeneous oil phase；It will go
Ionized water, antifreeze (can add to be not added with), thickener (can add to be not added with), defoamer are mixed, into homogeneous aqueous phase.In height
Under speed stirring, aqueous phase is added into oil phase, aqueous emulsion product of the present invention is made.Specifically it is shown in Table 5.
The each component of 5 embodiment of table 38~47 and content
The aqua of embodiment 48~50
By bacillus amyloliquefaciens, active component B, wetting agent, antifreeze (can add to be not added with), solvent, deionized water etc.
It is mixed together, the aqua product of the present invention is made.Specifically it is shown in Table 6.
The each component of 6 embodiment of table 48~50 and content
The microemulsion of embodiment 63~65
Bacillus amyloliquefaciens, active component B are dissolved in the homogenizer equipped with solvent, (can by emulsifying agent, antifreeze
Plus can be not added with), defoamer be added in the homogenizer equipped with above-mentioned solution, supplied and mixed strongly after surplus with deionized water
And homogenize, finally obtain the transparent microemulsion product of the present invention of clear appearance.Specifically it is shown in Table 6.
The each component of 6 embodiment of table 63~65 and content
The embodiment of the present invention is the method being combined using Toxicity Determination and field test.First pass through indoor virulence survey
Calmly, the synergy ratio (SR) after clear and definite two kinds of medicaments are compounded by a certain percentage, SR ＜ 0.5 are antagonism, 0.5≤SR≤1.5
For summation action, SR ＞ 1.5 are synergistic effect, on this basis, then carry out field test.
Test method：After determining the effective inhibition concentration scope of each medicament through prerun, medicament sets 5 respectively by active constituent content
Individual dosage processing, if clear water is compareed.Reference《Farm-chemical indoor determination test rule bactericide》Carry out, using mycelial growth speed
Rate method determines virulence of the medicament to crop germ.Colony diameter is measured with crossing method after 72h, the net growth of each processing is calculated
Amount, mycelial growth inhibition rate.
Net growth (mm)=measurement colony diameter -5
Application Example two：
For examination disease：Cucumber downy mildew；Test medicine：By Shaanxi, Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd carries
For；Experimental design：Bacillus amyloliquefaciens and active component B active compounds and the two different ratio mixture are determined by pilot study
Effective inhibition concentration scope.
Toxicity test result
The bacillus amyloliquefaciens of table 7 compound the toxicity test analysis of results table to cucumber downy mildew with polyoxin
As shown in Table 7, bacillus amyloliquefaciens prevent and treat cucumber downy mildew in bacillus amyloliquefaciens with polyoxin compounding
Weight range for the gemma of 2-700 hundred million/gram, when the weight range of polyoxin is 0.01~50%, synergy ratio SR is all higher than
1.5, illustrate both bacillus amyloliquefaciens weight range for the gemma of 2-700 hundred million/gram, the weight range of polyoxin is
Mixture shows synergistic effect in the range of 0.01~50%.
Application Example three：
For examination disease：Rice blast；Test medicine：By Shaanxi, Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd carries
For；Experimental design：Bacillus amyloliquefaciens and kasugarnycin active compound and the two different ratio mixture are determined by pilot study
Effective inhibition concentration scope.
Toxicity test result
The bacillus amyloliquefaciens of table 8 compound the toxicity test analysis of results table to rice blast with kasugarnycin
As shown in Table 8, bacillus amyloliquefaciens prevent and treat rice blast in bacillus amyloliquefaciens with kasugarnycin compounding
Weight range for the gemma of 2-700 hundred million/gram, when the weight range of kasugarnycin is 0.01~50%, synergy ratio SR is all higher than
1.5, illustrate both bacillus amyloliquefaciens weight range for the gemma of 2-700 hundred million/gram, the weight range of kasugarnycin is
Mixture shows synergistic effect in the range of 0.01~50%.
Application Example four：
For examination disease：Wheat powdery mildew；Test medicine：By Shaanxi, Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd carries
For；Experimental design：Bacillus amyloliquefaciens and zhongshengmycin bulk drug and the two different ratio mixture are determined by pilot study
Effective inhibition concentration scope.
Toxicity test result
The bacillus amyloliquefaciens of table 9 compound the toxicity test analysis of results table to wheat powdery mildew with Zhongshengmycin
As shown in Table 9, bacillus amyloliquefaciens prevent and treat wheat powdery mildew in bacillus amyloliquefaciens with Zhongshengmycin compounding
Weight range for the gemma of 2-700 hundred million/gram, when the weight range of Zhongshengmycin is 0.01~50%, synergy ratio SR is all higher than
1.5, illustrate both bacillus amyloliquefaciens weight range for the gemma of 2-700 hundred million/gram, the weight range of Zhongshengmycin is
Mixture shows synergistic effect in the range of 0.01~50%.
Application Example five：
For examination disease：Tomato virus disease；Test medicine：By Shaanxi, Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd carries
For；Experimental design：Bacillus amyloliquefaciens and Ningnanmycin active compound and the two different ratio mixture are determined by pilot study
Effective inhibition concentration scope.
Toxicity test result
The bacillus amyloliquefaciens of table 10 compound the toxicity test analysis of results table to tomato virus disease with Ningnanmycin
As shown in Table 10, bacillus amyloliquefaciens prevent and treat tomato virus disease in bacillus amyloliquefaciens with Ningnanmycin compounding
Weight range for the gemma of 2-700 hundred million/gram, when the weight range of Ningnanmycin is 0.01~50%, synergy ratio SR is all higher than
1.5, illustrate both bacillus amyloliquefaciens weight range for the gemma of 2-700 hundred million/gram, the weight range of Ningnanmycin is
Mixture shows synergistic effect in the range of 0.01~50%.
Application Example six：
For examination disease：Potato blight；Test medicine：By Shaanxi, Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd carries
For；Experimental design：Having for bacillus amyloliquefaciens and pesticide corrosion 120 active compound and the two different ratio mixture is determined by pilot study
Imitate inhibition concentration scope.
Toxicity test result
The bacillus amyloliquefaciens of table 11 compound the toxicity test analysis of results table to potato blight with pesticide corrosion 120
As shown in Table 11, bacillus amyloliquefaciens prevent and treat potato blight in bacillus amyloliquefaciens with pesticide corrosion 120 compounding
Weight range for the gemma of 2-700 hundred million/gram, when active component B weight range is 0.01~50%, synergy ratio SR is all higher than
1.5, illustrate both bacillus amyloliquefaciens weight range for the gemma of 2-700 hundred million/gram, active component B weight range is
Mixture shows synergistic effect in the range of 0.01~50%.
The bacillus amyloliquefaciens of Application Example seven and active component B and its compounding preventing and treating rice blast test of pesticide effectiveness
This test arrangement is in Anhui Province Fuyang City Yingshang County, and it is limited that test medicine founds rich biotechnology medicine company by Shaanxi Kang He
Company's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 3% Zhongshengmycin can
WP (purchased in market), 2% kasugarnycin aqua (purchased in market), 10% Wettable polyoxin powder (purchased in market), 8% Ningnanmycin
Aqua (purchased in market), 2% pesticide corrosion 120 aqua (purchased in market).
The rice blast state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 12 and active component B and its compounding preventing and treating rice blast test of pesticide effectiveness
As can be seen from Table 12, bacillus amyloliquefaciens are prevented with can effectively prevent and treat rice blast after active component B compoundings
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is long, and paddy rice leaf blight, damping-off can be prevented, effect is prevented and treated after 14 days
Fruit up to more than 97%.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example eight prevent and treat the apple brown rot test of pesticide effectiveness with active component B and its compounding
This test arrangement is in Xianyang, Shanxi province city Liquan County, and it is limited that test medicine founds rich biotechnology medicine company by Shaanxi Kang He
Company's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 3% Zhongshengmycin can
WP (purchased in market), 2% kasugarnycin aqua (purchased in market), 10% Wettable polyoxin powder (purchased in market), 8% Ningnanmycin
Aqua (purchased in market), 2% pesticide corrosion 120 aqua (purchased in market).
The apple brown rot state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 13 prevent and treat the apple brown rot test of pesticide effectiveness with active component B and its compounding
As can be seen from Table 13, bacillus amyloliquefaciens are prevented with can effectively prevent and treat apple brown rot after active component B compoundings
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is long, and alternaria leaf spot of apple, powdery mildew, 14 days rear defences can be prevented
Control effect and be up to more than 97%.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example nine and active component B and its compounding preventing and treating tomato virus disease test of pesticide effectiveness
This test arrangement is in Shaanxi Province Weinan City Dali County, and it is limited that test medicine founds rich biotechnology medicine company by Shaanxi Kang He
Company's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 3% Zhongshengmycin can
WP (purchased in market), 2% kasugarnycin aqua (purchased in market), 10% Wettable polyoxin powder (purchased in market), 8% Ningnanmycin
Aqua (purchased in market), 2% pesticide corrosion 120 aqua (purchased in market).
The tomato virus disease state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 14 and active component B and its compounding preventing and treating tomato virus disease test of pesticide effectiveness
As can be seen from Table 14, bacillus amyloliquefaciens are prevented with can effectively prevent and treat tomato virus disease after active component B compoundings
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is long, and graw mold of tomato can be prevented, prevention effect is up to after 14 days
More than 97%.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example ten prevent and treat the anthracnose of peach test of pesticide effectiveness with active component B and its compounding
This test arrangement founds rich biotechnology medicine in Xianyang, Shanxi province city besieged city Qiu Beidu towns, test medicine by Shaanxi Kang He
It is raw in the research and development of industry Co., Ltd, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 3%
It is rhzomorph wettable powder (purchased in market), 2% kasugarnycin aqua (purchased in market), 10% Wettable polyoxin powder (purchased in market), 8% peaceful
Southern mycin aqua (purchased in market), 2% pesticide corrosion 120 aqua (purchased in market).
The anthracnose of peach state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.End
7 days after secondary dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 15 prevent and treat the anthracnose of peach test of pesticide effectiveness with active component B and its compounding
As can be seen from Table 15, bacillus amyloliquefaciens are prevented and treated with can effectively prevent and treat anthracnose of peach after active component B compoundings
Effect is superior to the preventive effect of single dose, and preventive effect phase is long, and prevention effect is up to more than 97% after 14 days.It is right in experiment scope of medication
Target crop has no adverse effects.
The bacillus amyloliquefaciens of Application Example 11 are tried with active component B and its compounding preventing and treating cucumber downy mildew drug effect
Test
This test arrangement founds the rich limited public affairs of biotechnology medicine company in Xian District, Shanxi Province suburban area, test medicine by Shaanxi Kang He
Department's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 3% Zhongshengmycin are wettable
Property pulvis (purchased in market), 2% kasugarnycin aqua (purchased in market), 10% Wettable polyoxin powder (purchased in market), 8% Ningnanmycin water
Agent (purchased in market), 2% pesticide corrosion 120 aqua (purchased in market).
The cucumber downy mildew state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 16 and active component B and its compounding preventing and treating cucumber downy mildew test of pesticide effectiveness
As can be seen from Table 16, bacillus amyloliquefaciens are prevented with can effectively prevent and treat cucumber downy mildew after active component B compoundings
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is long.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example 12 and active component B and its sick drug effect of compounding preventing and treating potato blight
Experiment
This test arrangement is in Xi'an City, Shanxi Province Changan District, and it is limited that test medicine founds rich biotechnology medicine company by Shaanxi Kang He
Company's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 3% Zhongshengmycin can
WP (purchased in market), 2% kasugarnycin aqua (purchased in market), 10% Wettable polyoxin powder (purchased in market), 8% Ningnanmycin
Aqua (purchased in market), 2% pesticide corrosion 120 aqua (purchased in market).
The potato blight state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 17 and active component B and its compounding preventing and treating potato blight test of pesticide effectiveness
As can be seen from Table 17, bacillus amyloliquefaciens are prevented with can effectively prevent and treat potato blight after active component B compoundings
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is long.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example 13 are tried with active component B and its compounding preventing and treating capsicum black rot drug effect
Test
This test arrangement is in Shaanxi Province Weinan city Linwei district, and it is limited that test medicine founds rich biotechnology medicine company by Shaanxi Kang He
Company's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 3% Zhongshengmycin can
WP (purchased in market), 2% kasugarnycin aqua (purchased in market), 10% Wettable polyoxin powder (purchased in market), 8% Ningnanmycin
Aqua (purchased in market), 2% pesticide corrosion 120 aqua (purchased in market).
The capsicum black rot state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 18 and active component B and its compounding preventing and treating capsicum black rot test of pesticide effectiveness
As can be seen from Table 18, bacillus amyloliquefaciens are prevented with can effectively prevent and treat capsicum black rot after active component B compoundings
Control the preventive effect that effect is superior to single dose, and preventive effect phase is long, at the same to capsicum epidemic disease, powdery mildew preventive effect more than 97%.
Target crop is had no adverse effects in experiment scope of medication.
The experiment by different places throughout the country is drawn afterwards, and bacillus amyloliquefaciens are right after being compounded with active component B
Rice blast in various crop, anthracnose of peach, Pear black spot, apple brown rot, capsicum black rot, early blight, damping-off,
The preventive effect of the Common Diseases such as root rot, downy mildew, spot defoliation, epidemic disease, gray mold, powdery mildew, virosis 95% with
On, better than single dose preventive effect, synergistic effect is obvious.
Claims (6)
1. a kind of efficient bactericidal composite containing bacillus amyloliquefaciens, it is characterised in that effective active composition is solution starch gemma
Bacillus and active component B, the weight ranges of bacillus amyloliquefaciens for the gemma of 2-700 hundred million/gram, active component B weight range
For 0.01~50%, active component B is polyoxin, kasugarnycin, Ningnanmycin, Zhongshengmycin, antibiotic in pyrimidine nucleoside class.
2. the efficient bactericidal composite according to claim 1 containing bacillus amyloliquefaciens, it is characterised in that：Solve starch bud
The weight range of spore bacillus be the gemma of 5-500 hundred million/gram, active component B weight range is 0.05~30%.
3. the efficient bactericidal composite according to claim 2 containing bacillus amyloliquefaciens, it is characterised in that：Solve starch bud
The weight range of spore bacillus be the gemma of 7-400 hundred million/gram, active component B weight range is 0.1~20%.
4. the efficient bactericidal composite containing bacillus amyloliquefaciens according to any one in claims 1 to 3, its feature
It is：Wettable powder, water dispersible granules, suspending agent, suspension emulsion, aqua, aqueous emulsion, microemulsion is made in composition.
5. the efficient bactericidal composite according to claim 4 containing bacillus amyloliquefaciens is used to prevent and treat answering for crop disease
With.
6. application according to claim 5, it is characterised in that：Described disease is rice blast, and anthracnose of peach, pears are black
Pinta, apple brown rot, capsicum black rot, early blight, damping-off, root rot, downy mildew, spot defoliation, epidemic disease, grey mold
Disease, powdery mildew, virosis.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109717206A (en) * | 2018-08-30 | 2019-05-07 | 新疆农垦科学院 | A kind of composite bactericide suitable for the prevention and treatment of jujube black spot |
| CN110622997A (en) * | 2019-11-08 | 2019-12-31 | 四川龙蟒福生科技有限责任公司 | Bactericidal composition, application thereof and bactericide |
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| CN105707126A (en) * | 2016-03-29 | 2016-06-29 | 陕西先农生物科技有限公司 | Bactericidal composition, preparation and application of bactericidal composition |
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| CN105707126A (en) * | 2016-03-29 | 2016-06-29 | 陕西先农生物科技有限公司 | Bactericidal composition, preparation and application of bactericidal composition |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109717206A (en) * | 2018-08-30 | 2019-05-07 | 新疆农垦科学院 | A kind of composite bactericide suitable for the prevention and treatment of jujube black spot |
| CN110622997A (en) * | 2019-11-08 | 2019-12-31 | 四川龙蟒福生科技有限责任公司 | Bactericidal composition, application thereof and bactericide |
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Application publication date: 20170815 |