CN107047618A - A kind of bactericidal composition containing bacillus amyloliquefaciens - Google Patents
A kind of bactericidal composition containing bacillus amyloliquefaciens Download PDFInfo
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- CN107047618A CN107047618A CN201710299521.5A CN201710299521A CN107047618A CN 107047618 A CN107047618 A CN 107047618A CN 201710299521 A CN201710299521 A CN 201710299521A CN 107047618 A CN107047618 A CN 107047618A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention discloses a kind of bactericidal composition containing bacillus amyloliquefaciens, bactericidal composition containing active component bacillus amyloliquefaciens Yu active component B, and the weight range of bacillus amyloliquefaciens be 2 700 hundred million gemma/gram, active component B weight range for 2 700 hundred million gemma/gram.The present composition has greater activity to the multiple diseases in various crop, and with obvious synergistic effect, expands fungicidal spectrum.And with dosage is small, resistance of rainwater washing against, the characteristics of synergy is obvious.
Description
Technical field
The invention belongs to technical field of pesticide, it is related to a kind of bactericidal composition containing bacillus amyloliquefaciens in crop disease
On application.
Technical background
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), the bacillus amyloliquefaciens mechanism of action is main
Including secretion antibacterial material, antagonism, nutrition and the competition in space are produced, induction host produces resistance and promotes plant growth
Deng.
In the real process of agricultural production, controlling disease is easiest to generate the problem that the generation of disease drug resistance.No
Compounded with kind composition, be to prevent and treat resistance disease very common method.Heterogeneity is compounded, and is imitated according to practical application
Really, come judge certain compounding be synergy, plus and or antagonism.In most cases, the compounding effect of agricultural chemicals is all to add
And effect, really have the compounding of synergistic effect seldom, especially synergistic effect clearly, synergy ratio very high compounding just more
It is few.Studied by inventor, discovery mutually compounds bacillus amyloliquefaciens with active component B, has within the specific limits very
Good synergistic effect, and about bacillus amyloliquefaciens compounding related to active component B's, at present at home and abroad there is not yet related
Report.
The content of the invention
The purpose of the present invention is to propose to a kind of with synergistic function, the starch bud containing solution that use cost is low, preventive effect is good
The bactericidal composition of spore bacillus.
The present invention is to be achieved through the following technical solutions：
A kind of bactericidal composition containing bacillus amyloliquefaciens, containing bacillus amyloliquefaciens and active component B, solves starch
The weight range of bacillus be the gemma of 2-700 hundred million/gram, active component B weight range for the gemma of 2-700 hundred million/gram；Solve starch
The weight range of bacillus be the gemma of 5-500 hundred million/gram, active component B weight range for the gemma of 5-500 hundred million/gram；More preferably
For bacillus amyloliquefaciens weight range be the gemma of 7-400 hundred million/gram, active component B weight range for the gemma of 7-400 hundred million/
Gram, active component B is bacillus firmus, waxy Bacillus.
The described bactericidal composition containing bacillus amyloliquefaciens is used for the application for preventing and treating the disease of crop, described crop
Make including cereal crops, legume crop, fibre crops, sugar crop, melon crop, fruits crop, dry fruit crop, hobby
Thing, root crop, oil crops, flower crop, medicinal crop, crop, green manure pasture crop.
The described bactericidal composition containing bacillus amyloliquefaciens is used for the application for preventing and treating the disease of crop, described disease
Including for anthracnose, rice blast, black spot, brown rot, black rot, early blight, damping-off, root rot, late blight, downy mildew,
Scab, shot hole, anthrachose of grape, virosis, rot, spot defoliation, powdery mildew, leaf blight, banded sclerotial blight, droop, green grass or young crops
Rot, root-knot nematode, gray mold.
The amount of application when content of active component depends on being used alone in the present composition, also depends on a kind of chemical combination
Thing and the mixed ratio and synergistic effect degree of another compound, while also relevant with target disease.In usual composition
The weight percentage of active component is the 1%~90% of gross weight, preferably 5%~80%.According to different preparation classes
Type, active component content scope is different.Generally, liquid preparation contains by weight 1%~60% active material, preferably
Ground is 5%~50%；Solid pharmaceutical preparation contains by weight 5%~80% active material, preferably 10%~80%.
At least contain a kind of surfactant in the bactericidal composition of the present invention, active component is in water during in favor of applying
It is scattered.Surface-active contents are the 2%~30% of total formulation weight, and surplus is solid or liquid diluent.
Surfactant selected by the bactericidal composition of the present invention is known in those skilled in the art：It can be selected from
One or more in dispersant, wetting agent, thickener or defoamer.According to different dosage forms, this area can also be contained in preparation
Stabilizer, antifreeze well known to technical staff etc..
The present invention bactericidal composition can by user using it is preceding through dilute or directly use.Its preparation can be by ability
Prepared by the processing method well known to field technique personnel, i.e., after active component is mixed with liquid flux or solid carrier, add
One or more in surfactant such as dispersant, stabilizer, wetting agent, binding agent, defoamer.
The bactericidal composition of the present invention, acceptable formulation on any agricultural chemicals can be processed on demand.Wherein preferred agent
Type is wettable powder, water dispersible granules, suspending agent, suspension emulsion, microemulsion, aqueous emulsion, aqua.
Composition includes following component and content when wettable powder is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/
Gram, the gemma of active component B 2-700 hundred million/gram, dispersant 1%~12%, wetting agent 1%~8%, filler surplus.
Composition includes following component and content when water dispersible granules are made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/
Gram, the gemma of active component B2-700 hundred million/gram, it is dispersant 1%~12%, wetting agent 1%~8%, disintegrant 1%~10%, viscous
Tie agent 0~8%, filler surplus.
Composition includes following component and content when suspending agent is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, it is living
Property composition B 2-700 hundred million gemma/gram, dispersant 1%~10%, wetting agent 1%~10%, defoamer 0.01%~2%, thickening
Agent 0~2%, antifreeze 0~8%, deionized water add to 100%.
Composition includes following component and content when suspension emulsion is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, it is living
The gemma of property composition B2-700 hundred million/gram, emulsifying agent 1%~10%, dispersant 1%~10%, solvent 1%~20%, defoamer
0.01%~2%, thickener 0~2%, antifreeze 0~8%, deionized water add to 100%.
Composition includes following constituent content when aqua is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, activity into
Point gemma of B 2-700 hundred million/gram, cosolvent 2%~6%, wetting agent 1%-10%, antifreeze 0~8%, deionized water add to
100%.
Composition includes following component and content when aqueous emulsion is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, it is living
The gemma of property composition B 2-700 hundred million/gram, solvent 1%~20%, emulsifying agent 1%~12%, antifreeze 0~8%, defoamer
0.01%~2%, thickener 0~2%, deionized water add to 100%.
Composition includes following component and content when microemulsion is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, it is living
The gemma of property composition B 2-700 hundred million/gram, emulsifying agent 3%~25%, solvent 1%~10%, antifreeze 0~8%, defoamer
0.01%~2%, deionized water adds to 100%.
The wettable powder the key technical indexes of the present invention：
The water dispersible granules the key technical indexes of the present invention：
The suspending agent the key technical indexes of the present invention：
The suspension emulsion the key technical indexes of the present invention：
The aqua the key technical indexes of the present invention：
The aqueous emulsion the key technical indexes of the present invention：
The microemulsion the key technical indexes of the present invention：
The advantage of the invention is that：
(1) present composition has good synergy and lasting effect within the specific limits, and preventive effect is higher than single dose；(2) agriculture
Medicinal dose is reduced, residual quantity of the reduction agricultural chemicals on crop, mitigates environmental pollution；(3) fungicidal spectrum is expanded, to multiple diseases
Such as anthracnose, rice blast, black spot, brown rot, black rot, early blight, damping-off, root rot, late blight, downy mildew, black star
Disease, shot hole, anthrachose of grape, virosis, rot, spot defoliation, powdery mildew, leaf blight, banded sclerotial blight, droop, bacterial wilt,
Root-knot nematode, gray mold have greater activity.
Embodiment
With reference to embodiment to further instruction of the present invention, the percentage in embodiment is weight percentage, but
The invention is not limited in this.
Application Example one
The wettable powder of embodiment 1~6
Bacillus amyloliquefaciens, active component B, dispersant, wetting agent, filler are mixed, are well mixed in mixed cylinder,
Remixed after being crushed through airslide disintegrating mill uniform, you can wettable powder product of the present invention is made.Specifically it is shown in Table 1.
The each component of 1 embodiment of table 1~6 and content
The water dispersible granules of embodiment 7~12
Bacillus amyloliquefaciens, active component B, dispersant, wetting agent, disintegrant, filler are obtained through air-flow crushing together
To the particle diameter of needs, other auxiliary agents such as binding agent (can add to be not added with) are added, materials of pelletizing are obtained.Item is quantified into feeding stream
Change in bed granulating drier after granulation and drying, water dispersible granules product of the present invention is made.Specifically it is shown in Table 2.
The each component of 2 embodiment of table 7~12 and content
The suspending agent of embodiment 13~18
By dispersant, wetting agent, thickener (can add to be not added with), defoamer, antifreeze (can add to be not added with) by a high speed
Shear-mixed is uniform, adds bacillus amyloliquefaciens, active component B, supplies surplus with deionized water, ball milling 2 in ball mill
~3 hours, make diameter of particle all below 5 μm, suspending agent product of the present invention is made.Specifically it is shown in Table 3.
The each component of 3 embodiment of table 13~18 and content
The suspension emulsion of embodiment 19~20
By dispersant, defoamer, thickener (can add to be not added with), antifreeze (can add to be not added with), deionized water through too high
Fast shear-mixed is uniform, adds bacillus amyloliquefaciens, ball milling 2~3 hours in ball mill make diameter of particle all at 5 μm
Hereinafter, bacillus amyloliquefaciens suspending agent is made, then by active component B, solvent, emulsifying agent and various auxiliary agent high-speed stirreds
Device is directly emulsified into suspending agent, and suspension emulsion product of the present invention is made.Specifically it is shown in Table 4.
The each component of 4 embodiment of table 19~20 and content
The aqueous emulsion of embodiment 21~24
Bacillus amyloliquefaciens, active component B, solvent, emulsifying agent are added together, make to be dissolved into homogeneous oil phase；It will go
Ionized water, antifreeze (can add to be not added with), thickener (can add to be not added with), defoamer are mixed, into homogeneous aqueous phase.In height
Under speed stirring, aqueous phase is added into oil phase, aqueous emulsion product of the present invention is made.Specifically it is shown in Table 5.
The each component of 5 embodiment of table 21~24 and content
The aqua of embodiment 25~30
By bacillus amyloliquefaciens, active component B, wetting agent, antifreeze (can add to be not added with), solvent, deionized water etc.
It is mixed together, the aqua product of the present invention is made.Specifically it is shown in Table 6.
The each component of 6 embodiment of table 25~30 and content
The microemulsion of embodiment 31~33
Bacillus amyloliquefaciens, active component B are dissolved in the homogenizer equipped with solvent, (can by emulsifying agent, antifreeze
Plus can be not added with), defoamer be added in the homogenizer equipped with above-mentioned solution, supplied and mixed after surplus and even with deionized water
Change, finally obtain the transparent microemulsion product of the present invention of clear appearance.Specifically it is shown in Table 6.
The each component of 6 embodiment of table 31~33 and content
The embodiment of the present invention is the method being combined using Toxicity Determination and field test.First pass through indoor virulence survey
Calmly, the synergy ratio (SR) after clear and definite two kinds of medicaments are compounded by a certain percentage, SR ＜ 0.5 are antagonism, 0.5≤SR≤1.5
For summation action, SR ＞ 1.5 are synergistic effect, on this basis, then carry out field test.
Test method：After determining the effective inhibition concentration scope of each medicament through prerun, medicament sets 5 respectively by active constituent content
Individual dosage processing, if clear water is compareed.Reference《Farm-chemical indoor determination test rule bactericide》Carry out, using mycelial growth speed
Rate method determines virulence of the medicament to crop germ.Colony diameter is measured with crossing method after 72h, the net growth of each processing is calculated
Amount, mycelial growth inhibition rate.
Net growth (mm)=measurement colony diameter -5
Application Example two：
For examination disease：Cucumber downy mildew；Test medicine：By Shaanxi, Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd carries
For；Experimental design：Bacillus amyloliquefaciens and active component B active compounds and the two different ratio mixture are determined by pilot study
Effective inhibition concentration scope.
Toxicity test result
The bacillus amyloliquefaciens of table 7 compound the toxicity test analysis of results table to cucumber downy mildew with bacillus firmus
As shown in Table 7, bacillus amyloliquefaciens are solving starch gemma with bacillus firmus compounding preventing and treating cucumber downy mildew
The weight range of bacillus be the gemma of 2-700 hundred million/gram, the weight range of bacillus firmus for the gemma of 2-700 hundred million/gram when, synergy
Ratio SR is all higher than 1.5, illustrate both bacillus amyloliquefaciens weight range for the gemma of 2-700 hundred million/gram, strong gemma bar
The weight range of bacterium be the gemma of 2-700 hundred million/gram in the range of mixture show synergistic effect.
Application Example three：
For examination disease：Rice blast；Test medicine：By Shaanxi, Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd carries
For；Experimental design：Determine that bacillus amyloliquefaciens and waxy Bacillus active compound and the two different ratio are mixed by pilot study
Effective inhibition concentration scope of agent.
Toxicity test result
The bacillus amyloliquefaciens of table 8 compound the toxicity test analysis of results table to rice blast with waxy Bacillus
As shown in Table 8, bacillus amyloliquefaciens are solving starch gemma with waxy Bacillus compounding preventing and treating rice blast
The weight range of bacillus be the gemma of 2-700 hundred million/gram, the weight range of waxy Bacillus for the gemma of 2-700 hundred million/gram when, synergy
Ratio SR is all higher than 1.5, illustrate both bacillus amyloliquefaciens weight range for the gemma of 2-700 hundred million/gram, wax gemma bar
The weight range of bacterium be the gemma of 2-700 hundred million/gram in the range of mixture show synergistic effect.
The bacillus amyloliquefaciens of Application Example four are tried with active component B and its sick drug effect of compounding preventing and treating tomato root-knot eelworm
Test
This test arrangement is in Shaanxi Province Weinan City Dali County, and it is limited that test medicine founds rich biotechnology medicine company by Shaanxi Kang He
Company's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 2,000,000,000 gemma/gram wax
Matter bacillus wettable powder (purchased in market), 2,500,000,000 gemma/gram bacillus firmus wettable powder (purchased in market).
The sick state of an illness of tomato root-knot eelworm is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2
It is secondary.7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 9 and active component B and its sick test of pesticide effectiveness of compounding preventing and treating tomato root-knot eelworm
As can be seen from Table 9, bacillus amyloliquefaciens after active component B compoundings with can effectively prevent and treat tomato root-knot eelworm
Disease, prevention effect is superior to the preventive effect of single dose, and preventive effect phase is long.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example five and active component B and its compounding preventing and treating walnut anthracnose test of pesticide effectiveness
This test arrangement is ground in Xianyang, Shanxi province city, test medicine by Shaanxi Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd
Hair, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 2,000,000,000 gemma/gram wax gemma
Bacillus wettable powder (purchased in market), 2,500,000,000 gemma/gram bacillus firmus wettable powder (purchased in market).
The walnut anthracnose state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 10 and active component B and its compounding preventing and treating walnut anthracnose test of pesticide effectiveness
As can be seen from Table 10, bacillus amyloliquefaciens are prevented with can effectively prevent and treat walnut anthracnose after active component B compoundings
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is long.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example six and active component B and its compounding preventing and treating tomato virus disease test of pesticide effectiveness
This test arrangement is in Shaanxi Province Weinan City Dali County, and it is limited that test medicine founds rich biotechnology medicine company by Shaanxi Kang He
Company's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 2,000,000,000 gemma/gram wax
Matter bacillus wettable powder (purchased in market), 2,500,000,000 gemma/gram bacillus firmus wettable powder (purchased in market).
The tomato virus disease state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 11 and active component B and its compounding preventing and treating tomato virus disease test of pesticide effectiveness
As can be seen from Table 11, bacillus amyloliquefaciens are prevented with can effectively prevent and treat tomato virus disease after active component B compoundings
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is long.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example seven and active component B and its compounding preventing and treating rice blast test of pesticide effectiveness
This test arrangement is in Anhui Province Fuyang City Yingshang County, and it is limited that test medicine founds rich biotechnology medicine company by Shaanxi Kang He
Company's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 2,000,000,000 gemma/gram wax
Matter bacillus wettable powder (purchased in market), 2,500,000,000 gemma/gram bacillus firmus wettable powder (purchased in market).
The rice blast state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 12 and active component B and its compounding preventing and treating rice blast test of pesticide effectiveness
As can be seen from Table 12, bacillus amyloliquefaciens are prevented with can effectively prevent and treat rice blast after active component B compoundings
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is long, and rice green smut, banded sclerotial blight, bacterial wilt can be treated, after 14 days
Prevention effect is up to more than 97%.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example eight and active component B and its compounding preventing and treating gray mold of cucumber test of pesticide effectiveness
This test arrangement founds the rich limited public affairs of biotechnology medicine company in Xian District, Shanxi Province suburban area, test medicine by Shaanxi Kang He
Department's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 2,000,000,000 gemma/gram wax
Bacillus wettable powder (purchased in market), 2,500,000,000 gemma/gram bacillus firmus wettable powder (purchased in market).
The gray mold of cucumber state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 13 and active component B and its compounding preventing and treating gray mold of cucumber test of pesticide effectiveness
As can be seen from Table 13, bacillus amyloliquefaciens are prevented with can effectively prevent and treat gray mold of cucumber after active component B compoundings
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is long.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example nine and active component B and its sick test of pesticide effectiveness of compounding preventing and treating capsicum epidemic disease
This test arrangement is in Xi'an City, Shanxi Province Changan District, and it is limited that test medicine founds rich biotechnology medicine company by Shaanxi Kang He
Company's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 2,000,000,000 gemma/gram wax
Matter bacillus wettable powder (purchased in market), 2,500,000,000 gemma/gram bacillus firmus wettable powder (purchased in market).
The capsicum epidemic disease state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.End
7 days after secondary dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 14 and active component B and its compounding preventing and treating capsicum epidemic disease test of pesticide effectiveness
As can be seen from Table 14, bacillus amyloliquefaciens are prevented and treated with can effectively prevent and treat capsicum epidemic disease after active component B compoundings
Effect is superior to the preventive effect of single dose, and preventive effect phase is long.Target crop is had no adverse effects in experiment scope of medication.
The experiment by different places throughout the country is drawn afterwards, and bacillus amyloliquefaciens are right after being compounded with active component B
Anthracnose, rice blast, black spot, brown rot, black rot in various crop, early blight, damping-off, root rot, late blight, frost
It is mildew, scab, shot hole, anthrachose of grape, virosis, rot, spot defoliation, powdery mildew, leaf blight, banded sclerotial blight, withered
The preventive effect of the Common Diseases such as disease, bacterial wilt, root-knot nematode, gray mold is more than 97%, and better than single dose preventive effect, synergistic effect is bright
It is aobvious.
Claims (6)
1. a kind of bactericidal composition containing bacillus amyloliquefaciens, it is characterised in that effective active composition is bacillus amyloliquefaciens
With active component B, the weight ranges of bacillus amyloliquefaciens for the gemma of 2-700 hundred million/gram, active component B weight range is 2-
70000000000 gemma/gram, active component B is bacillus firmus, waxy Bacillus.
2. the bactericidal composition according to claim 1 containing bacillus amyloliquefaciens, it is characterised in that：Solve starch gemma bar
The weight range of bacterium be the gemma of 5-500 hundred million/gram, active component B weight range for the gemma of 5-500 hundred million/gram.
3. the bactericidal composition according to claim 2 containing bacillus amyloliquefaciens, it is characterised in that：Solve starch gemma bar
The weight range of bacterium be the gemma of 7-400 hundred million/gram, active component B weight range for the gemma of 7-400 hundred million/gram.
4. the bactericidal composition containing bacillus amyloliquefaciens according to any one in claims 1 to 3, its feature exists
In：Wettable powder, water dispersible granules, suspending agent, suspension emulsion, aqua, aqueous emulsion, microemulsion is made in composition.
5. the bactericidal composition according to claim 4 containing bacillus amyloliquefaciens is used for the application for preventing and treating crop disease.
6. application according to claim 5, it is characterised in that：Described disease is anthracnose, rice blast, black spot, brown
Maize ear rot, black rot, early blight, damping-off, root rot, late blight, downy mildew, scab, shot hole, anthrachose of grape, virosis, corruption
Rotten disease, spot defoliation, powdery mildew, leaf blight, banded sclerotial blight, droop, bacterial wilt, root-knot nematode, gray mold.
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| CN113061559A (en) * | 2021-05-10 | 2021-07-02 | 南京农业大学 | Composite biological agent and application thereof in preventing and treating rice blast and promoting rice growth |
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