CN107006519A - A kind of efficient bactericidal composite containing bacillus amyloliquefaciens - Google Patents
A kind of efficient bactericidal composite containing bacillus amyloliquefaciens Download PDFInfo
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- CN107006519A CN107006519A CN201710255168.0A CN201710255168A CN107006519A CN 107006519 A CN107006519 A CN 107006519A CN 201710255168 A CN201710255168 A CN 201710255168A CN 107006519 A CN107006519 A CN 107006519A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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Abstract
The invention discloses a kind of efficient bactericidal composite containing bacillus amyloliquefaciens, bactericidal composition containing active component bacillus amyloliquefaciens Yu active component B, and the weight range of bacillus amyloliquefaciens be 2 700 hundred million gemma/gram, active component B weight range is 0.01~50%.The present composition has greater activity to the multiple diseases in various crop, and with obvious synergistic effect, expands fungicidal spectrum.And with dosage is small, resistance of rainwater washing against, the characteristics of synergy is obvious.
Description
Technical field
The invention belongs to technical field of pesticide, it is related to a kind of efficient bactericidal composite containing bacillus amyloliquefaciens in crop
Application in disease.
Technical background
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), the bacillus amyloliquefaciens mechanism of action is main
Including secretion antibacterial material, antagonism, nutrition and the competition in space are produced, induction host produces resistance and promotes plant growth
Deng.
In the real process of agricultural production, controlling disease is easiest to generate the problem that the generation of disease drug resistance.No
Compounded with kind composition, be to prevent and treat resistance disease very common method.Heterogeneity is compounded, and is imitated according to practical application
Really, come judge certain compounding be synergy, plus and or antagonism.In most cases, the compounding effect of agricultural chemicals is all to add
And effect, really have the compounding of synergistic effect seldom, especially synergistic effect clearly, synergy ratio very high compounding just more
It is few.Studied by inventor, discovery mutually compounds bacillus amyloliquefaciens with active component B, has within the specific limits very
Good synergistic effect, and about bacillus amyloliquefaciens compounding related to active component B's, at present at home and abroad there is not yet related
Report.
The content of the invention
The purpose of the present invention is to propose to a kind of with synergistic function, the starch bud containing solution that use cost is low, preventive effect is good
The efficient bactericidal composite of spore bacillus.
The present invention is to be achieved through the following technical solutions：
A kind of efficient bactericidal composite containing bacillus amyloliquefaciens, contains bacillus amyloliquefaciens and active component B, solution
The weight range of bacillus amyloliquefacienses be the gemma of 2-700 hundred million/gram, active component B weight range is 0.01~50%；Solve starch
The weight range of bacillus be the gemma of 5-500 hundred million/gram, active component B weight range is 0.05~30%；More preferably solve
The weight range of bacillus amyloliquefacienses be the gemma of 7-400 hundred million/gram, active component B weight range is 0.1~20%, activity into
It is benziothiazolinone, amino-oligosaccharide, lentinan, tetramycin to divide B.
The described bactericidal composition containing bacillus amyloliquefaciens is used for the application for preventing and treating the disease of crop, described crop
Make including cereal crops, legume crop, fibre crops, sugar crop, melon crop, fruits crop, dry fruit crop, hobby
Thing, root crop, oil crops, flower crop, medicinal crop, crop, green manure pasture crop.
The described bactericidal composition containing bacillus amyloliquefaciens is used for the application for preventing and treating the disease of crop, described disease
It is early blight, damping-off, root rot, late blight, downy mildew, black including rice blast, anthracnose, black spot, brown rot, black rot
Star disease, shot hole, anthrachose of grape, virosis, rot, spot defoliation, powdery mildew, leaf blight, banded sclerotial blight, droop, green grass or young crops are withered
Disease, gray mold.
The amount of application when content of active component depends on being used alone in the present composition, also depends on a kind of chemical combination
Thing and the mixed ratio and synergistic effect degree of another compound, while also relevant with target disease.In usual composition
The weight percentage of active component is the 1%~90% of gross weight, preferably 5%~80%.According to different preparation classes
Type, active component content scope is different.Generally, liquid preparation contains by weight 1%~60% active material, preferably
Ground is 5%~50%；Solid pharmaceutical preparation contains by weight 5%~80% active material, preferably 10%~80%.
At least contain a kind of surfactant in the bactericidal composition of the present invention, active component is in water during in favor of applying
It is scattered.Surface-active contents are the 2%~30% of total formulation weight, and surplus is solid or liquid diluent.
Surfactant selected by the bactericidal composition of the present invention is known in those skilled in the art：It can be selected from
One or more in dispersant, wetting agent, thickener or defoamer.According to different dosage forms, this area can also be contained in preparation
Stabilizer, antifreeze well known to technical staff etc..
The present invention bactericidal composition can by user using it is preceding through dilute or directly use.Its preparation can be by ability
Prepared by the processing method well known to field technique personnel, i.e., after active component is mixed with liquid flux or solid carrier, add
One or more in surfactant such as dispersant, stabilizer, wetting agent, binding agent, defoamer.
The bactericidal composition of the present invention, acceptable formulation on any agricultural chemicals can be processed on demand.Wherein preferred agent
Type is wettable powder, water dispersible granules, suspending agent, suspension emulsion, microemulsion, aqueous emulsion, aqua.
Composition includes following component and content when wettable powder is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/
Gram, active component B 0.01%~50%, dispersant 1%~12%, wetting agent 1%~8%, filler surplus.
Composition includes following component and content when water dispersible granules are made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/
Gram, active component B 0.01%~50%, dispersant 1%~12%, wetting agent 1%~8%, disintegrant 1%~10%, bond
Agent 0~8%, filler surplus.
Composition includes following component and content when suspending agent is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, it is living
Property composition B 0.01%~50%, dispersant 1%~10%, wetting agent 1%~10%, defoamer 0.01%~2%, thickener
0~2%, antifreeze 0~8%, deionized water add to 100%.
Composition includes following component and content when suspension emulsion is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, it is living
Property composition B 0.01%~50%, emulsifying agent 1%~10%, dispersant 1%~10%, solvent 1%~20%, defoamer
0.01%~2%, thickener 0~2%, antifreeze 0~8%, deionized water add to 100%.
Composition includes following constituent content when aqua is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, activity into
B 0.01%~50%, cosolvent 2%~6%, wetting agent 1%-10%, antifreeze 0~8%, deionized water is divided to add to
100%.
Composition includes following component and content when aqueous emulsion is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, it is living
Property composition B 0.01%~50%, solvent 1%~20%, emulsifying agent 1%~12%, antifreeze 0~8%, defoamer 0.01%
~2%, thickener 0~2%, deionized water add to 100%.
Composition includes following component and content when microemulsion is made：The gemma of bacillus amyloliquefaciens 2-700 hundred million/gram, it is living
Property composition B 0.01%~50%, emulsifying agent 3%~25%, solvent 1%~10%, antifreeze 0~8%, defoamer 0.01%
~2%, deionized water adds to 100%.
The wettable powder the key technical indexes of the present invention：
The water dispersible granules the key technical indexes of the present invention：
The suspending agent the key technical indexes of the present invention：
The suspension emulsion the key technical indexes of the present invention：
The aqua the key technical indexes of the present invention：
The aqueous emulsion the key technical indexes of the present invention：
The microemulsion the key technical indexes of the present invention：
The advantage of the invention is that：
(1) present composition has good synergy and lasting effect within the specific limits, and preventive effect is higher than single dose；(2) agriculture
Medicinal dose is reduced, residual quantity of the reduction agricultural chemicals on crop, mitigates environmental pollution；(3) fungicidal spectrum is expanded, to multiple diseases
Such as rice blast, anthracnose, black spot, brown rot, black rot, early blight, damping-off, root rot, late blight, downy mildew, black star
Disease, shot hole, anthrachose of grape, virosis, rot, spot defoliation, powdery mildew, leaf blight, banded sclerotial blight, droop, bacterial wilt,
Gray mold has greater activity.
Embodiment
With reference to embodiment to further instruction of the present invention, the percentage in embodiment is weight percentage, but
The invention is not limited in this.
Application Example one
The wettable powder of embodiment 1~12
Bacillus amyloliquefaciens, active component B, dispersant, wetting agent, filler are mixed, are well mixed in mixed cylinder,
Remixed after being crushed through airslide disintegrating mill uniform, you can wettable powder product of the present invention is made.Specifically it is shown in Table 1.
The each component of 1 embodiment of table 1~12 and content
The water dispersible granules of embodiment 13~20
Bacillus amyloliquefaciens, active component B, dispersant, wetting agent, disintegrant, filler are obtained through air-flow crushing together
To the particle diameter of needs, other auxiliary agents such as binding agent (can add to be not added with) are added, materials of pelletizing are obtained.Item is quantified into feeding stream
Change in bed granulating drier after granulation and drying, water dispersible granules product of the present invention is made.Specifically it is shown in Table 2.
The each component of 2 embodiment of table 13~20 and content
The suspending agent of embodiment 21~28
By dispersant, wetting agent, thickener (can add to be not added with), defoamer, antifreeze (can add to be not added with) by a high speed
Shear-mixed is uniform, adds bacillus amyloliquefaciens, active component B, supplies surplus with deionized water, ball milling 2 in ball mill
~3 hours, make diameter of particle all below 5 μm, suspending agent product of the present invention is made.Specifically it is shown in Table 3.
The each component of 3 embodiment of table 21~28 and content
The suspension emulsion of embodiment 29~30
By dispersant, defoamer, thickener (can add to be not added with), antifreeze (can add to be not added with), deionized water through too high
Fast shear-mixed is uniform, adds bacillus amyloliquefaciens, ball milling 2~3 hours in ball mill make diameter of particle all at 5 μm
Hereinafter, bacillus amyloliquefaciens suspending agent is made, then by active component B, solvent, emulsifying agent and various auxiliary agent high-speed stirreds
Device is directly emulsified into suspending agent, and suspension emulsion product of the present invention is made.Specifically it is shown in Table 4.
The each component of 4 embodiment of table 29~30 and content
The aqueous emulsion of embodiment 31~38
Bacillus amyloliquefaciens, active component B, solvent, emulsifying agent are added together, make to be dissolved into homogeneous oil phase；It will go
Ionized water, antifreeze (can add to be not added with), thickener (can add to be not added with), defoamer are mixed, into homogeneous aqueous phase.In height
Under speed stirring, aqueous phase is added into oil phase, aqueous emulsion product of the present invention is made.Specifically it is shown in Table 5.
The each component of 5 embodiment of table 31~38 and content
The aqua of embodiment 39~50
By bacillus amyloliquefaciens, active component B, wetting agent, antifreeze (can add to be not added with), solvent, deionized water etc.
It is mixed together, the aqua product of the present invention is made.Specifically it is shown in Table 6.
The each component of 6 embodiment of table 39~50 and content
The microemulsion of embodiment 51~53
Bacillus amyloliquefaciens, active component B are dissolved in the homogenizer equipped with solvent, (can by emulsifying agent, antifreeze
Plus can be not added with), defoamer be added in the homogenizer equipped with above-mentioned solution, supplied and mixed after surplus and even with deionized water
Change, finally obtain the transparent microemulsion product of the present invention of clear appearance.Specifically it is shown in Table 6.
The each component of 6 embodiment of table 51~53 and content
The embodiment of the present invention is the method being combined using Toxicity Determination and field test.First pass through indoor virulence survey
Calmly, the synergy ratio (SR) after clear and definite two kinds of medicaments are compounded by a certain percentage, SR ＜ 0.5 are antagonism, 0.5≤SR≤1.5
For summation action, SR ＞ 1.5 are synergistic effect, on this basis, then carry out field test.
Test method：After determining the effective inhibition concentration scope of each medicament through prerun, medicament sets 5 respectively by active constituent content
Individual dosage processing, if clear water is compareed.Reference《Farm-chemical indoor determination test rule bactericide》Carry out, using mycelial growth speed
Rate method determines virulence of the medicament to crop germ.Colony diameter is measured with crossing method after 72h, the net growth of each processing is calculated
Amount, mycelial growth inhibition rate.
Net growth (mm)=measurement colony diameter -5
Application Example two：
For examination disease：Cucumber downy mildew；Test medicine：By Shaanxi, Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd carries
For；Experimental design：Bacillus amyloliquefaciens and active component B active compounds and the two different ratio mixture are determined by pilot study
Effective inhibition concentration scope.
Toxicity test result
The bacillus amyloliquefaciens of table 7 compound the toxicity test analysis of results table to cucumber downy mildew with benziothiazolinone
| Reagent agent | Proportioning | Synergy ratio (SR) |
| Bacillus amyloliquefaciens | — | — |
| Benziothiazolinone | — | — |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 4000000000 gemma/gram+2% | 1.78 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 10000000000 gemma/gram+0.1% | 2.01 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 3000000000 gemma/gram+0.2% | 2.14 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 6000000000 gemma/gram+1.2% | 2.21 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 3000000000 gemma/gram+2% | 2.36 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 3000000000 gemma/gram+1.5% | 2.29 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 20000000000 gemma/gram+0.2% | 2.24 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 4500000000 gemma/gram+1.5% | 2.13 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 40000000000 gemma/gram+0.3% | 2.08 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 2000000000 gemma/gram+0.5% | 2.11 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 30000000000 gemma/gram+2% | 2.02 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 10500000000 gemma/gram+1% | 1.99 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 12000000000 gemma/gram+0.5% | 1.84 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 45000000000 gemma/gram+1% | 1.81 |
| Solve shallow lake powder bacillus ︰ benziothiazolinone | 3000000000 gemma/gram+5% | 1.95 |
As shown in Table 7, bacillus amyloliquefaciens prevent and treat cucumber downy mildew in bacillus amyloliquefaciens with benziothiazolinone compounding
Weight range be the gemma of 2-700 hundred million/gram, when the weight range of benziothiazolinone is 0.01~50%, synergy ratio SR is all higher than 1.5,
Illustrate both bacillus amyloliquefaciens weight range for the gemma of 2-700 hundred million/gram, the weight range of benziothiazolinone for 0.01~
Mixture shows synergistic effect in the range of 50%.
Application Example three：
For examination disease：Rice blast；Test medicine：By Shaanxi, Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd carries
For；Experimental design：Bacillus amyloliquefaciens and amino-oligosaccharide active compound and the two different ratio mixture are determined by pilot study
Effective inhibition concentration scope.
Toxicity test result
The bacillus amyloliquefaciens of table 8 compound the toxicity test analysis of results table to rice blast with amino-oligosaccharide
| Reagent agent | Proportioning | Synergy ratio (SR) |
| Bacillus amyloliquefaciens | — | — |
| Amino-oligosaccharide | — | — |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 8000000000 gemma/gram+0.7% | 1.85 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 3000000000 gemma/gram+2% | 1.91 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 1000000000 gemma/gram+0.5% | 1.98 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 4000000000 gemma/gram+0.5% | 2.03 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 10000000000 gemma/gram+0.4% | 2.09 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 1500000000 gemma/gram+0.2% | 2.11 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 3000000000 gemma/gram+0.6% | 2.17 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 2200000000 gemma/gram+1% | 2.24 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 3000000000 gemma/gram+2% | 2.35 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 3000000000 gemma/gram+2% | 2.21 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 1000000000 gemma/gram+1.5% | 2.19 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 2000000000 gemma/gram+2.5% | 2.12 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 12000000000 gemma/gram+0.5% | 2.05 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 45000000000 gemma/gram+1% | 1.84 |
| Solve shallow lake powder bacillus ︰ amino-oligosaccharides | 3000000000 gemma/gram+5% | 1.71 |
As shown in Table 8, bacillus amyloliquefaciens are solving starch gemma bar with amino-oligosaccharide compounding preventing and treating rice blast
The weight range of bacterium be the gemma of 2-700 hundred million/gram, when the weight range of amino-oligosaccharide is 0.01~50%, synergy ratio SR is equal
More than 1.5, illustrate both bacillus amyloliquefaciens weight range for the gemma of 2-700 hundred million/gram, the weight model of amino-oligosaccharide
Enclose and show synergistic effect for mixture in the range of 0.01~50%.
Application Example four：
For examination disease：Wheat powdery mildew；Test medicine：By Shaanxi, Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd carries
For；Experimental design：Having for bacillus amyloliquefaciens and tetramycin active compound and the two different ratio mixture is determined by pilot study
Imitate inhibition concentration scope.
Toxicity test result
The bacillus amyloliquefaciens of table 9 compound the toxicity test analysis of results table to wheat powdery mildew with tetramycin
| Reagent agent | Proportioning | Synergy ratio (SR) |
| Bacillus amyloliquefaciens | — | — |
| Tetramycin | — | — |
| Solve shallow lake powder bacillus ︰ tetramycins | 10500000000 gemma/gram+0.2% | 1.84 |
| Solve shallow lake powder bacillus ︰ tetramycins | 6000000000 gemma/gram+1% | 1.91 |
| Solve shallow lake powder bacillus ︰ tetramycins | 7000000000 gemma/gram+2.5% | 1.99 |
| Solve shallow lake powder bacillus ︰ tetramycins | 2000000000 gemma/gram+1.5% | 2.02 |
| Solve shallow lake powder bacillus ︰ tetramycins | 30000000000 gemma/gram+2% | 2.08 |
| Solve shallow lake powder bacillus ︰ tetramycins | 4500000000 gemma/gram+0.3% | 2.14 |
| Solve shallow lake powder bacillus ︰ tetramycins | 10000000000 gemma/gram+0.5% | 2.17 |
| Solve shallow lake powder bacillus ︰ tetramycins | 10500000000 gemma/gram+0.8% | 2.22 |
| Solve shallow lake powder bacillus ︰ tetramycins | 2000000000 gemma/gram+0.6% | 2.39 |
| Solve shallow lake powder bacillus ︰ tetramycins | 5000000000 gemma/gram+2% | 2.41 |
| Solve shallow lake powder bacillus ︰ tetramycins | 4000000000 gemma/gram+0.4% | 2.35 |
| Solve shallow lake powder bacillus ︰ tetramycins | 2000000000 gemma/gram+1.5% | 2.28 |
| Solve shallow lake powder bacillus ︰ tetramycins | 12000000000 gemma/gram+0.5% | 2.21 |
| Solve shallow lake powder bacillus ︰ tetramycins | 45000000000 gemma/gram+1% | 2.15 |
| Solve shallow lake powder bacillus ︰ tetramycins | 3000000000 gemma/gram+5% | 2.06 |
As shown in Table 9, bacillus amyloliquefaciens prevent and treat wheat powdery mildew in bacillus amyloliquefaciens with tetramycin compounding
Weight range be the gemma of 2-700 hundred million/gram, when the weight range of tetramycin is 0.01~50%, synergy ratio SR is all higher than 1.5,
Illustrate both bacillus amyloliquefaciens weight range for the gemma of 2-700 hundred million/gram, the weight range of tetramycin for 0.01~
Mixture shows synergistic effect in the range of 50%.
Application Example five：
For examination disease：Tomato virus disease；Test medicine：By Shaanxi, Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd carries
For；Experimental design：Bacillus amyloliquefaciens and lentinan active compound and the two different ratio mixture are determined by pilot study
Effective inhibition concentration scope.
Toxicity test result
The bacillus amyloliquefaciens of table 10 compound the toxicity test analysis of results table to tomato virus disease with lentinan
As shown in Table 10, bacillus amyloliquefaciens prevent and treat tomato virus disease in bacillus amyloliquefaciens with lentinan compounding
Weight range for the gemma of 2-700 hundred million/gram, when the weight range of lentinan is 0.01~50%, synergy ratio SR is all higher than
1.5, illustrate both bacillus amyloliquefaciens weight range for the gemma of 2-700 hundred million/gram, the weight range of lentinan is
Mixture shows synergistic effect in the range of 0.01~50%.
The bacillus amyloliquefaciens of Application Example six and active component B and its compounding preventing and treating rice blast test of pesticide effectiveness
This test arrangement founds the rich limited public affairs of biotechnology medicine company in ChangSha, Hunan Province suburban area, test medicine by Shaanxi Kang He
Department's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 0.3% tetramycin aqua
(purchased in market), 2% amino-oligosaccharide aqua (purchased in market), 5% benziothiazolinone suspending agent (purchased in market), 2% lentinan aqua (purchased in market).
The rice blast state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 11 and active component B and its compounding preventing and treating rice blast test of pesticide effectiveness
As can be seen from Table 11, bacillus amyloliquefaciens are prevented with can effectively prevent and treat rice blast after active component B compoundings
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is long.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example seven and active component B and its compounding preventing and treating apple peels and cores medicine
Effect experiment
This test arrangement is ground in Xianyang, Shanxi province city, test medicine by Shaanxi Kang He Li Feng biotechnologies pharmaceutcal corporation, Ltd
Hair, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 0.3% tetramycin aqua (city
Purchase), 2% amino-oligosaccharide aqua (purchased in market), 5% benziothiazolinone suspending agent (purchased in market), 2% lentinan aqua (purchased in market).
The apple peels and cores state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is applied altogether
Medicine 2 times.7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 12 and active component B and its compounding preventing and treating apple peels and cores test of pesticide effectiveness
As can be seen from Table 13, bacillus amyloliquefaciens fall with energy effectively preventing and treating apple-tree spot after active component B compoundings
Leaf disease, prevention effect is superior to the preventive effect of single dose, and preventive effect phase is long.To target crop without bad shadow in experiment scope of medication
Ring.
The bacillus amyloliquefaciens of Application Example nine and active component B and its compounding preventing and treating tomato virus disease test of pesticide effectiveness
This test arrangement is in Shaanxi Province Weinan City Dali County, and it is limited that test medicine founds rich biotechnology medicine company by Shaanxi Kang He
Company's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 0.3% tetramycin water
Agent (purchased in market), 2% amino-oligosaccharide aqua (purchased in market), 5% benziothiazolinone suspending agent (purchased in market), 2% lentinan aqua (purchased in market).
The tomato virus disease state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 14 and active component B and its compounding preventing and treating tomato virus disease test of pesticide effectiveness
As can be seen from Table 14, bacillus amyloliquefaciens are prevented with can effectively prevent and treat tomato virus disease after active component B compoundings
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is long.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example ten and active component B and its compounding preventing and treating uncinula necator test of pesticide effectiveness
This test arrangement founds the rich limited public affairs of biotechnology medicine company in Shaanxi Province Weinan suburban area, test medicine by Shaanxi Kang He
Department's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 0.3% tetramycin aqua
(purchased in market), 2% amino-oligosaccharide aqua (purchased in market), 5% benziothiazolinone suspending agent (purchased in market), 2% lentinan aqua (purchased in market).
The uncinula necator state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 15 and active component B and its sick test of pesticide effectiveness of compounding preventing and treating uncinula necator
As can be seen from Table 15, bacillus amyloliquefaciens are prevented with can effectively prevent and treat uncinula necator after active component B compoundings
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is long, and grape grey mould can be treated, prevention effect is up to after 14 days
More than 97%.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example 11 and active component B and its compounding preventing and treating brassicaceous vegetable downy mildew
The sick test of pesticide effectiveness
This test arrangement founds the rich limited public affairs of biotechnology medicine company in Xian District, Shanxi Province suburban area, test medicine by Shaanxi Kang He
Department's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 0.3% tetramycin aqua
(purchased in market), 2% amino-oligosaccharide aqua (purchased in market), 5% benziothiazolinone suspending agent (purchased in market), 2% lentinan aqua (purchased in market).
The downy mildew of crucifers state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days is once, common
Dispenser 2 times.7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 16 prevent and treat the downy mildew of crucifers test of pesticide effectiveness with active component B and its compounding
As can be seen from Table 16, bacillus amyloliquefaciens after active component B compoundings with can effectively prevent and treat brassicaceous vegetable
Downy mildew, prevention effect is superior to the preventive effect of single dose, and preventive effect phase is long.To target crop without bad shadow in experiment scope of medication
Ring.
The bacillus amyloliquefaciens of Application Example 12 are tried with active component B and its sick drug effect of compounding preventing and treating capsicum epidemic disease
Test
This test arrangement is in Xi'an City, Shanxi Province Changan District, and it is limited that test medicine founds rich biotechnology medicine company by Shaanxi Kang He
Company's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 0.3% tetramycin water
Agent (purchased in market), 2% amino-oligosaccharide aqua (purchased in market), 5% benziothiazolinone suspending agent (purchased in market), 2% lentinan aqua (purchased in market).
The capsicum epidemic disease state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.End
7 days after secondary dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 17 and active component B and its compounding preventing and treating capsicum epidemic disease test of pesticide effectiveness
As can be seen from Table 17, bacillus amyloliquefaciens are prevented and treated with can effectively prevent and treat capsicum epidemic disease after active component B compoundings
Effect is superior to the preventive effect of single dose, and preventive effect phase is long.Target crop is had no adverse effects in experiment scope of medication.
The bacillus amyloliquefaciens of Application Example 13 are tried with active component B and its compounding preventing and treating rice blast drug effect
Test
This test arrangement founds the rich limited public affairs of biotechnology medicine company in Shaanxi Province Hanzhong suburban area, test medicine by Shaanxi Kang He
Department's research and development, offer, the gemma of comparison medicament 1,000,000,000/gram bacillus amyloliquefaciens wettable powder (purchased in market), 0.3% tetramycin aqua
(purchased in market), 2% amino-oligosaccharide aqua (purchased in market), 5% benziothiazolinone suspending agent (purchased in market), 2% lentinan aqua (purchased in market).
The rice blast state of an illness is investigated before medicine, in state of an illness first time at initial stage dispenser, every dispenser in 7 days once, is total to dispenser 2 times.
7 days after last dispenser, disease index is investigated respectively and preventive effect is calculated within 14 days, 30 days.Experimental result is as follows：
The bacillus amyloliquefaciens of table 18 and active component B and its compounding preventing and treating rice blast test of pesticide effectiveness
The experiment by different places throughout the country is drawn afterwards, and bacillus amyloliquefaciens are right after being compounded with active component B
Rice blast, anthracnose in various crop, black spot, brown rot, black rot, early blight, damping-off, root rot, late blight, frost
It is mildew, scab, shot hole, anthrachose of grape, virosis, rot, spot defoliation, powdery mildew, leaf blight, banded sclerotial blight, withered
The preventive effect of the Common Diseases such as disease, bacterial wilt, gray mold is more than 97%, and better than single dose preventive effect, synergistic effect is obvious.
Claims (6)
1. a kind of efficient bactericidal composite containing bacillus amyloliquefaciens, it is characterised in that effective active composition is solution starch gemma
Bacillus and active component B, the weight ranges of bacillus amyloliquefaciens for the gemma of 2-700 hundred million/gram, active component B weight range
For 0.01~50%, active component B is benziothiazolinone, amino-oligosaccharide, lentinan, tetramycin.
2. the efficient bactericidal composite according to claim 1 containing bacillus amyloliquefaciens, it is characterised in that：Solve starch bud
The weight range of spore bacillus be the gemma of 5-500 hundred million/gram, active component B weight range is 0.05~30%.
3. the efficient bactericidal composite according to claim 2 containing bacillus amyloliquefaciens, it is characterised in that：Solve starch bud
The weight range of spore bacillus be the gemma of 7-400 hundred million/gram, active component B weight range is 0.1~20%.
4. the efficient bactericidal composite containing bacillus amyloliquefaciens according to any one in claims 1 to 3, its feature
It is：Wettable powder, water dispersible granules, suspending agent, suspension emulsion, aqua, aqueous emulsion, microemulsion is made in composition.
5. the efficient bactericidal composite according to claim 4 containing bacillus amyloliquefaciens is used to prevent and treat answering for crop disease
With.
6. application according to claim 5, it is characterised in that：Described disease is rice blast, anthracnose, black spot, brown
Maize ear rot, black rot, early blight, damping-off, root rot, late blight, downy mildew, scab, shot hole, anthrachose of grape, virosis, corruption
Rotten disease, spot defoliation, powdery mildew, leaf blight, banded sclerotial blight, droop, bacterial wilt, gray mold.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107518006A (en) * | 2017-08-25 | 2017-12-29 | 河南中麦生物科技有限公司 | A kind of bacterium solution extremely brought back to life for vegetables and fruit leaf and its application |
| CN110063190A (en) * | 2019-03-18 | 2019-07-30 | 田阳县鲜达康果蔬专业合作社 | A kind of method for integrated control of mango disease and insect |
| CN110810090A (en) * | 2019-12-05 | 2020-02-21 | 广西壮族自治区农业科学院 | Method for preventing and controlling powdery mildew of 'Guipu No. 6' winter grape cultivated in two-harvest mode |
| CN115710144A (en) * | 2022-12-01 | 2023-02-24 | 贵州省烟草公司贵阳市公司 | Pesticide-fertilizer mixed combination for preventing and treating tobacco target spot disease and application thereof |
| CN115812719A (en) * | 2022-12-08 | 2023-03-21 | 宁夏大学 | Compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application of compound composition in promoting growth of cucumber seedlings |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107518006A (en) * | 2017-08-25 | 2017-12-29 | 河南中麦生物科技有限公司 | A kind of bacterium solution extremely brought back to life for vegetables and fruit leaf and its application |
| CN110063190A (en) * | 2019-03-18 | 2019-07-30 | 田阳县鲜达康果蔬专业合作社 | A kind of method for integrated control of mango disease and insect |
| CN110810090A (en) * | 2019-12-05 | 2020-02-21 | 广西壮族自治区农业科学院 | Method for preventing and controlling powdery mildew of 'Guipu No. 6' winter grape cultivated in two-harvest mode |
| CN115710144A (en) * | 2022-12-01 | 2023-02-24 | 贵州省烟草公司贵阳市公司 | Pesticide-fertilizer mixed combination for preventing and treating tobacco target spot disease and application thereof |
| CN115710144B (en) * | 2022-12-01 | 2024-04-30 | 贵州省烟草公司贵阳市公司 | Pesticide-fertilizer mixed combination for preventing and treating tobacco target spot disease and application thereof |
| CN115812719A (en) * | 2022-12-08 | 2023-03-21 | 宁夏大学 | Compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application of compound composition in promoting growth of cucumber seedlings |
| CN115812719B (en) * | 2022-12-08 | 2024-03-01 | 宁夏大学 | Compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application of compound composition in promoting cucumber seedling growth |
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Application publication date: 20170804 |