CN115812719A - Compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application of compound composition in promoting growth of cucumber seedlings - Google Patents
Compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application of compound composition in promoting growth of cucumber seedlings Download PDFInfo
- Publication number
- CN115812719A CN115812719A CN202211572787.XA CN202211572787A CN115812719A CN 115812719 A CN115812719 A CN 115812719A CN 202211572787 A CN202211572787 A CN 202211572787A CN 115812719 A CN115812719 A CN 115812719A
- Authority
- CN
- China
- Prior art keywords
- treatment
- chitosan oligosaccharide
- lentinan
- bacillus amyloliquefaciens
- compound composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical group O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 title claims abstract description 116
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 title claims abstract description 77
- 229920001491 Lentinan Polymers 0.000 title claims abstract description 75
- 229940115286 lentinan Drugs 0.000 title claims abstract description 75
- 241000193744 Bacillus amyloliquefaciens Species 0.000 title claims abstract description 62
- 230000012010 growth Effects 0.000 title claims abstract description 56
- 150000001875 compounds Chemical class 0.000 title claims abstract description 52
- 239000000203 mixture Substances 0.000 title claims abstract description 52
- 230000001737 promoting effect Effects 0.000 title claims abstract description 14
- 244000299906 Cucumis sativus var. sativus Species 0.000 title claims 2
- 239000003085 diluting agent Substances 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 240000008067 Cucumis sativus Species 0.000 abstract description 77
- 230000000694 effects Effects 0.000 abstract description 47
- 238000010672 photosynthesis Methods 0.000 abstract description 7
- 230000029553 photosynthesis Effects 0.000 abstract description 7
- 235000015097 nutrients Nutrition 0.000 abstract description 6
- 230000021749 root development Effects 0.000 abstract description 5
- 239000011159 matrix material Substances 0.000 abstract description 3
- 239000002689 soil Substances 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- 230000006872 improvement Effects 0.000 abstract description 2
- 230000002508 compound effect Effects 0.000 abstract 1
- 238000011282 treatment Methods 0.000 description 182
- 239000000243 solution Substances 0.000 description 64
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 26
- 241000196324 Embryophyta Species 0.000 description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 102000004190 Enzymes Human genes 0.000 description 13
- 108090000790 Enzymes Proteins 0.000 description 13
- 229930002875 chlorophyll Natural products 0.000 description 13
- 235000019804 chlorophyll Nutrition 0.000 description 13
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 13
- 238000003973 irrigation Methods 0.000 description 13
- 230000002262 irrigation Effects 0.000 description 13
- 229910052757 nitrogen Inorganic materials 0.000 description 13
- 238000012360 testing method Methods 0.000 description 11
- 238000013329 compounding Methods 0.000 description 9
- 230000000243 photosynthetic effect Effects 0.000 description 8
- 229920001661 Chitosan Polymers 0.000 description 7
- 239000000758 substrate Substances 0.000 description 7
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 6
- 230000007423 decrease Effects 0.000 description 6
- 229940118019 malondialdehyde Drugs 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000009825 accumulation Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 239000005416 organic matter Substances 0.000 description 5
- 230000005068 transpiration Effects 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 4
- 229920001542 oligosaccharide Polymers 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000011284 combination treatment Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000005728 strengthening Methods 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 235000009849 Cucumis sativus Nutrition 0.000 description 2
- 244000186654 Fockea angustifolia Species 0.000 description 2
- 235000015210 Fockea angustifolia Nutrition 0.000 description 2
- 239000006137 Luria-Bertani broth Substances 0.000 description 2
- 230000003625 amylolytic effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- LHGVFZTZFXWLCP-UHFFFAOYSA-N guaiacol Chemical compound COC1=CC=CC=C1O LHGVFZTZFXWLCP-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000010413 mother solution Substances 0.000 description 2
- JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 2
- 150000002482 oligosaccharides Chemical class 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000002786 root growth Effects 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- RVBUGGBMJDPOST-UHFFFAOYSA-N 2-thiobarbituric acid Chemical compound O=C1CC(=O)NC(=S)N1 RVBUGGBMJDPOST-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 229960001867 guaiacol Drugs 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000010496 root system development Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application thereof in promoting growth of cucumber seedlings belong to the technical field of agricultural planting, the compound composition is in a liquid form, wherein the concentration of the chitosan oligosaccharide is 100-700mg/L, and the viable count of the bacillus amyloliquefaciens is 10 5 ‑10 8 cfu/mL, wherein the lentinan is 400-1000 times of lentinan diluent. The compound composition can effectively promote the growth vigor, root development and photosynthesis of cucumber seedlings, has a promoting effect obviously greater than that of a chitosan oligosaccharide solution or a bacillus amyloliquefaciens culture solution or a lentinan solution which is independently applied, is superior to the compound effect of the chitosan oligosaccharide solution and the bacillus amyloliquefaciens, and shows an obvious synergistic effect.Meanwhile, the compound composition has a more remarkable effect on improvement of matrix soil nutrients, can remarkably relieve the harm of low-temperature stress on the growth vigor of cucumber seedlings, and improves the low-temperature resistance of the cucumber seedlings.
Description
Technical Field
The invention belongs to the technical field of agricultural planting, and particularly relates to a compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application thereof in promoting growth of cucumber seedlings.
Background
The biological source substance acts on plants, shows obvious stress resistance and growth promotion effects, and is harmless to the environment and agricultural products. The research shows that biological source substances such as biological stimulants, plant immunity inducers, biological bactericides and the like have particularly outstanding capability of improving the stress resistance and the quality of crops.
The cucumber is one of the main vegetables planted in China, the planting area of the cucumber is greatly increased in recent years, in order to increase the yield of the cucumber, a large amount of chemical fertilizer and pesticide is applied, the environment and the quality of the cucumber are polluted and damaged, the quality of the cucumber is obviously reduced, meanwhile, the growth of the cucumber is in a sub-health state, and the sustainable development of the cucumber industry is not facilitated. The existing single biological source substance has limited promotion effect on the growth of the cucumber, so that research and development of a composite biological source preparation capable of obviously improving the quality of the cucumber have important significance on sustainable development of the cucumber facility cultivation industry.
Disclosure of Invention
In view of the above, the invention aims to provide a compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan capable of effectively promoting the growth of cucumber seedlings, and provide a new theoretical basis and technical means for improving the comprehensive production capacity of cucumbers.
The technical scheme adopted by the invention is as follows:
the invention provides a compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan, which is in a liquid form, wherein the concentration of the chitosan oligosaccharide is 100-700mg/L, and the viable count of the bacillus amyloliquefaciens is 10 5 -10 8 cfu/mL, wherein the lentinan is 400-1000 times of lentinan diluent.
Preferably, the concentration of the chitosan oligosaccharide is 500mg/L, and the viable count of the bacillus amyloliquefaciens is 10 7 cfu/mL, wherein the lentinan is 700 times of lentinan diluent.
The invention also provides application of the compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan in promoting growth of cucumber seedlings.
Has the advantages that: the compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan provided by the invention can effectively promote the growth vigor, root development and photosynthesis of cucumber seedlings. The field test shows that the promoting effect of the compound composition is obviously greater than the effect of singly applying the chitosan oligosaccharide solution or the amylolytic bacillus culture solution or the lentinan solution, and simultaneously the compound composition is superior to the compound of the chitosan oligosaccharide solution and the amylolytic bacillus culture solution, and the compound composition shows obvious synergistic effect. The analysis of the combination test results shows that the reason why the compound composition has the beneficial growth promoting effect is as follows: the chitosan oligosaccharide in the compound composition can enhance the activity index of plant cells, promote the growth of root systems and stems and leaves of crops, increase the content of chlorophyll and simultaneously improve the photosynthesis efficiency; lentinan can promote the plant height, stem thickness and chlorophyll content of cucumber seedlings to be increased; the bacillus amyloliquefaciens and the chitosan oligosaccharide have interaction, the bacillus amyloliquefaciens can secrete chitosan enzyme to form chitosan oligosaccharide with higher biological activity so as to enhance the biological activity of the chitosan oligosaccharide, and the chitosan oligosaccharide can promote the proliferation of the bacillus amyloliquefaciens, is beneficial to the colonization of the bacillus amyloliquefaciens on root systems of cucumber seedlings and promotes the activation of substrate nutrients and the root system development of the cucumbers.
In addition, the compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan has a more remarkable effect on improving the nutrients of the matrix soil; after root irrigation treatment is carried out on the compound composition, the damage of low-temperature stress to the growth vigor of cucumber seedlings is remarkably relieved, the low-temperature resistance of the cucumber seedlings is improved, and the compound composition has important significance for cultivating strong cucumber seedlings and ensuring safe overwintering of the cucumber seedlings.
Drawings
FIG. 1 shows the effect of different concentrations of chitooligosaccharide treatment on the growth vigor of cucumber seedlings.
FIG. 2 shows the effect of treatment with different concentrations of chito-oligosaccharide on biomass accumulation of cucumber seedlings.
FIG. 3 shows the effect of treatment of the compounded composition on the growth vigor of cucumber seedlings under low temperature stress.
FIG. 4 shows the effect of treatment of a formulated composition on biomass accumulation in cucumber seedlings under low temperature stress.
FIG. 5 shows the effect of treatment of the compounded composition on cucumber seedling antioxidant enzyme activity under low temperature stress.
FIG. 6 shows the effect of treatment of the compounded composition on the malondialdehyde content of cucumber seedlings under low temperature stress.
FIG. 7 is a graph showing the growth of Bacillus amyloliquefaciens XY-13 under chitosan oligosaccharide.
Detailed Description
The technical solutions and effects of the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings of the present invention.
The test methods used in the following examples are all conventional methods unless otherwise specified; the sources of the materials for this test are as follows:
lentinan is purchased from Beijing Sanpu Baicao Green plant preparation, inc., and is an aqueous solution with an effective component content of 1%.
The chitosan oligosaccharide to be tested is provided by the university of eight agricultural cultivations in Heilongjiang.
The Bacillus amyloliquefaciens is Bacillus amyloliquefaciens XY-13 (CCTCC M2021270) provided by microbiological laboratory of Ningxia university.
The invention provides a compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan, which is in a liquid form, wherein the concentration of the chitosan oligosaccharide is 100-700mg/L, and the viable count of the bacillus amyloliquefaciens is 10 5 -10 8 cfu/mL, wherein the lentinan is 400-1000 times of lentinan diluent.
Preferably, the concentration of the chitosan oligosaccharide is 500mg/L, and the viable count of the bacillus amyloliquefaciens is 10 7 cfu/mL, wherein the lentinan is 700 times of lentinan diluent.
The invention also provides application of the compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan in promoting cucumber seedling growth.
The compound composition is liquid, and the preparation method takes the bacillus amyloliquefaciens culture solution with the preset thallus concentration as mother solution, and chitosan oligosaccharide and lentinan with preset quantity are respectively added into the mother solution, so that the concentration of the chitosan oligosaccharide and the lentinan in the compound system reaches the preset value. Taking 1L of compound composition liquid as an example, 0.5g of chitosan oligosaccharide and 1.42ml of lentinan are dissolved in 1L of 10 7 Preparing 500mg/L chitosan oligosaccharide +10 mg/L in cfu/mL bacillus amyloliquefaciens XY-13 bacterial liquid 7 cfu/mL bacillus amyloliquefaciens XY-13+700 times lentinan mixed solution; when preparing single chitosan oligosaccharide or lentinan diluent, dissolving with 1L clear water; when two kinds of complex combinations are prepared, 1L of bacterial solution is used for dissolving if the bacterial agent exists.
The growth promoting effect of the compound composition on cucumber seedlings is illustrated by specific experiments.
Example 1 growth promotion test of Chitosan oligosaccharide on cucumber seedlings
In the test, one-leaf one-heart Dell LD-1 cucumber seedlings are used as test materials, no additional fertilizer is applied during the seedling raising period, water is uniformly irrigated according to the treatment of the growth requirements of crops during the seedling raising period, and 1 liter of water is irrigated in one hole tray (72 holes) at a time. The concentration of the chitosan oligosaccharide is set as 100mg/L, 200mg/L, 300mg/L, 400mg/L, 500mg/L, 600mg/L and 700mg/L, clear water irrigation is used as a control, 3 times of root irrigation treatment are respectively carried out on the 1 st day, the 10 th day and the 20 th day of the treatment beginning, 8 treatments are carried out in total, each treatment is repeated for 3 times, 40 plants are treated, each plant is irrigated for 15mL, the water management is unified for all the treatments, and the seedling growth indexes are counted.
Treatment 1: blank control, irrigation with clear water only
And (3) treatment 2: pouring chitosan oligosaccharide solution with the concentration of 100mg/L
And (3) treatment: pouring chitosan oligosaccharide solution with the concentration of 200mg/L
And (4) treatment: pouring chitosan oligosaccharide solution with the concentration of 300mg/L
And (4) treatment 5: pouring chitosan oligosaccharide solution with the concentration of 400mg/L
And (6) treatment: pouring chitosan oligosaccharide solution with the concentration of 500mg/L
And (7) treatment: pouring chitosan oligosaccharide solution with the concentration of 600mg/L
And (4) treatment 8: pouring chitosan oligosaccharide solution with the concentration of 700mg/L
As shown in figure 1, the relative growth rate of plant height, the relative growth rate of stem thickness, the chlorophyll content and the seedling strengthening index of the chitosan oligosaccharide treatment are all obviously higher than those of the blank Control (CK) treatment, and the plant height, the stem thickness, the chlorophyll content and the seedling strengthening index of the chitosan oligosaccharide treatment show a trend of increasing first and then decreasing along with the increase of the concentration of the chitosan oligosaccharide, wherein the treatment performance is optimal when the concentration of the chitosan oligosaccharide solution is 500mg/L (K500). Compared with CK treatment, when the concentration of the chitosan oligosaccharide solution is 500mg/L, the relative growth rate of plant height, the relative growth rate of stem thickness, the chlorophyll content and the strong seedling index of the treated plants are obviously improved by 39.5%, 87.9%, 17.8% and 70.8%.
As shown in FIG. 2, the fresh weight of the chitosan oligosaccharide-treated overground part, the dry weight and the fresh weight of the underground part are both significantly higher than those of the blank control treatment, and the dry weight of the chitosan oligosaccharide-treated overground part is significantly higher than that of the blank Control (CK) treatment, wherein the concentrations of the chitosan oligosaccharide are 400mg/L, 500mg/L and 600mg/L (K400, K500 and K600). Wherein the dry and fresh weight of the underground part treated by K500 is optimal, and is increased by 43.2%, 70.8%, 77.1% and 105.9% relative to CK. By integrating various growth indexes, the 500mg/L chitosan oligosaccharide solution can obviously improve the growth vigor of cucumber seedlings and promote the accumulation of biomass.
TABLE 1 Effect of treatment with Chitosan oligosaccharides at different concentrations on the chemistry of the substrate
As can be seen from Table 1, the nutrient contents of the substrate tend to increase and decrease with the increase of the concentration of the chitosan oligosaccharide. The content of quick-acting nitrogen, quick-acting potassium and total nitrogen in the chitosan oligosaccharide solution treated by 500mg/L is the highest compared with that in other treatments, and the content of quick-acting nitrogen and total nitrogen is obviously higher than that in blank control treatment by 156.9 percent and 20 percent. There was no significant difference between treatments for other indicators. In conclusion, the nutrient improvement effect is optimal when the root is irrigated with 500mg/L chitosan oligosaccharide solution.
TABLE 2 Effect of treatment with Chitosan oligosaccharide of different concentrations on root growth characteristics of cucumber seedlings
As can be seen from table 2, the length, surface area and volume of the roots of the chitosan oligosaccharide treatment are significantly higher than those of the blank control treatment, and the chitosan oligosaccharide concentration increases and then decreases. Wherein, the 500mg/L chitosan oligosaccharide solution treatment has the optimal performance, is obviously higher than other treatments, and the root diameters of the treatments have no obvious difference. Compared with blank control treatment, the length, surface area and volume of roots treated by 500mg/L chitosan oligosaccharide solution are obviously improved by 59.1%, 106.1% and 134.3%.
TABLE 3 Effect of treatment with Chitosan oligosaccharide at different concentrations on the photosynthetic Properties of cucumber seedlings
As can be seen from Table 3, the transpiration rate between the treatments was zeroThe difference is significant. The net photosynthetic rate and stomatal conductance of the chitosan oligosaccharide treatment are obviously higher than those of the blank control treatment, and intercellular CO 2 All concentrations were higher than the blank. Wherein the net photosynthetic rate, intercellular CO, of the 500mg/L treatment of the Chitosan oligosaccharide solution 2 The concentration and the porosity conductivity are optimal, and are obviously improved by 121.0%, 15.6% and 21.1% compared with blank control treatment.
Example 2 growth promotion test of compounding bacillus amyloliquefaciens and chitosan oligosaccharide on cucumber seedlings
The experiments were performed with 500mg/L chitosan oligosaccharide in combination with Bacillus amyloliquefaciens. The concentration of the bacillus amyloliquefaciens is set to be 10 5 cfu/mL、10 6 cfu/ml、10 7 cfu/ml、10 8 cfu/ml, selecting the bacillus amyloliquefaciens XY-13 which is screened by the laboratory and effectively promotes the growth of cucumber seedlings. Using pure water root irrigation as a control, performing 3 root irrigation treatments on the 1 st day, the 10 th day and the 20 th day of the beginning of the treatment respectively, setting 11 treatments, repeating 3 times for each treatment, filling 40 plants for each treatment, filling 15ml of liquid for each plant, and measuring the growth vigor of seedlings on the 30 th day.
Treatment 1: blank control, irrigation with clear water only
And (3) treatment 2:500mg/L chitosan oligosaccharide solution
And (3) treatment: 10 7 cfu/ml XY-13 culture solution
And (4) treatment: 500mg/L Chitosan oligosaccharide solution +10 5 cfu/ml XY-13 culture solution
And (4) treatment 5:500mg/L Chitosan oligosaccharide solution +10 6 cfu/ml XY-13 culture solution
And (6) treatment: 500mg/L Chitosan oligosaccharide solution +10 7 cfu/ml XY-13 culture solution
And (7) treatment: 500mg/L Chitosan oligosaccharide solution +10 8 cfu/ml XY-13 culture solution
Table 4 influence of the combination of Bacillus amyloliquefaciens and chitosan oligosaccharide on growth vigor of cucumber seedlings
As can be seen from Table 4, each growth index of the compound treatment of chitosan oligosaccharide and Bacillus amyloliquefaciens is obviously higher than that of the blank control treatment, and meanwhile, the relative growth rate of stem thickness, the chlorophyll content and the seedling strengthening index are obviously higher than those of the single chitosan oligosaccharide treatment; the relative growth rate of stem thickness and the strong seedling index are obviously higher than those of single bacillus amyloliquefaciens treatment. Treatment 6 (500 mg/L Chitosan oligosaccharide + 10) in the compounding treatment 7 cfu/ml XY-13) has the highest plant height relative growth rate, stem thickness relative growth rate and chlorophyll content, which are obviously higher than that of blank control treatment by 50%, 100% and 53.8%.
TABLE 5 influence of the combination of Bacillus amyloliquefaciens and chitooligosaccharide on the root development of cucumber seedlings
As can be seen from table 5, the root development index of each combination treatment was significantly improved compared to the blank control treatment. The root volume and root diameter of each compound treatment are obviously higher than those of the single chitosan oligosaccharide treatment and the single bacillus amyloliquefaciens treatment. Treatment 6 (500 mg/L Chitosan oligosaccharide + 10) in the combination treatment as compared with treatment 1 7 cfu/ml XY-13) root volume, root diameter, 261.4%, 53.3% higher.
TABLE 6 Effect of compounding Bacillus amyloliquefaciens with Chitosan oligosaccharide on photosynthesis of cucumber seedlings
As can be seen from Table 6, the transpiration rate, net photosynthetic rate, and stomatal conductance of each formulation treatment were significantly higher than those of treatment 1. Treatment 6 (500 mg/L chito-oligosaccharide + 10) in the compounding treatment 7 cfu/ml XY-13) showed the best transpiration rate and net photosynthetic rate, which were 227.3% and 134.0% higher than those of treatment 1. The porosity conductance of treatment 7 was the highest, 300.0% higher than that of treatment 1.
TABLE 7 Effect of different treatments on the substrate chemistry
As can be seen from Table 7, the combination of chitosan oligosaccharide and Bacillus amyloliquefaciens increased the chemical property content of the substrate except for the fast-acting phosphorus, relative to treatment 1. The content of organic matter and quick-acting nitrogen of the compounded combination is obviously higher than that of the blank control treatment. Wherein, 6 (500 mg/L chitosan oligosaccharide + 10) is treated in the compound treatment 7 cfu/ml XY-13) is obviously higher than that of other treatments in EC, organic matter, quick-acting nitrogen, quick-acting potassium and total nitrogen content, and compared with treatment 1, the contents of the organic matter, the quick-acting nitrogen and the total nitrogen are obviously higher by 79.1%, 380.2% and 30.15%.
Example 3 growth promotion test of compounding bacillus amyloliquefaciens, chitosan oligosaccharide and lentinan on cucumber seedlings
The method comprises the steps of disinfecting cucumber seeds, soaking the seeds, then culturing the seedlings, and carrying out root irrigation treatment on a chitosan oligosaccharide solution, a bacillus amyloliquefaciens XY-13 (CCTCC M2021270) culture solution, a lentinan solution and a compound composition in a one-leaf one-core period of the cucumber seedlings, wherein 15ml of liquid is poured into each hole by taking a 72-hole tray as an example. Irrigating roots once every 10 days for three times, and counting the seedling growth indexes. The concentration of lentinan is 400 times, 700 times and 1000 times. Using pure water root irrigation as a control, 3 root irrigation treatments were performed on the 1 st day, the 10 th day and the 20 th day of the initiation of the treatment, 11 treatments were set, 3 times of each treatment were repeated, 40 plants were treated, and the growth vigor of the seedlings was measured on the 30 th day.
Treatment 1: blank control, irrigation with clear water only
And (3) treatment 2:400 times of lentinan diluent
And (3) treatment: 700 times of lentinan diluent
And (4) treatment: 1000 times lentinan diluent
And (4) treatment 5:500mg/L Chitosan oligosaccharide solution +10 7 cfu/ml XY-13 culture solution
And (6) treatment: 500mg/L Chitosan oligosaccharide solution +10 7 cfu/ml XY-13+400 times lentinan diluent
And (7) treatment: 500mgL Chitosan oligosaccharide solution +10 7 Dilution of lentinan of cfu/ml XY-13+700 times
And (4) treatment 8:500mg/L Chitosan oligosaccharide solution +10 7 Dilution of lentinan 1000 times cfu/ml XY-13+
TABLE 8 influence of Bacillus amyloliquefaciens, chitosan oligosaccharide and lentinan compounding on growth vigor of cucumber seedlings
As can be seen from Table 8, the lentinan treatment at different concentrations, 500mg/L of the chitosan oligosaccharide solution +10 7 cfu/ml XY-13, and 500mg/L Chitosan oligosaccharide solution +10 7 The plant height relative growth rate, stem thickness relative growth rate and chlorophyll content of cfu/ml XY-13 and lentinan with different concentrations are all obviously higher than those of the blank control treatment. The relative growth rate of plant height between other treatments was not significantly different except for the blank control treatment. The growth indicators of lentinan with different concentrations in the treatment process (treatment 2-4) and the growth indicators of the lentinan with different concentrations in the treatment process 5 have no obvious difference. Treatment 7 (500 mg/L Chitosan oligosaccharide solution + 10) relative to treatment 1 7 cfu/ml XY-13+700 times lentinan diluent) has the highest relative growth rate and strong seedling index, which are remarkably higher than 61.4% and 20.8%; treatment 8 (500 mg/L Chitosan oligosaccharide solution + 10) 7 cfu/ml XY-13+1000 times lentinan diluent), the relative growth rate and chlorophyll content of the treated plant are highest, the relative treatment 1 is higher by 38.2 percent and 69.1 percent, and the treatment effect 7 is better than the single lentinan treatment, chitosan oligosaccharide solution and bacillus amyloliquefaciens compound treatment.
TABLE 9 influence of the combination of Bacillus amyloliquefaciens, chitosan oligosaccharide and lentinan on the root development of cucumber seedlings
As can be seen from Table 9, the root growth indexes of treatments 5 to 8 were all significantly higher than those of treatment 1. The length, surface area and volume of the roots of the lentinan with different concentrations are obviously higher than those of the roots treated by blank control. Among them, treatment 7 (500 mg/L Chitosan oligosaccharide solution + 10) 7 cfu/ml XY-13+700 times lentinan diluent) has the best effects on root length, root surface area, root diameter and root volume, and is 47.7%, 140.2%, 32.5% and 171.3% higher than that of treatment 1.
TABLE 10 influence of Bacillus amyloliquefaciens, chitosanoligosaccharide and lentinan compounding on photosynthesis of cucumber seedlings
As can be seen from Table 10, the transpiration rate and the net photosynthetic rate of the treatment 5 and the combination treatment (treatments 6 to 8) with lentinan were significantly higher than those of the treatment 1. The net photosynthetic rate of lentinan treatment at different concentrations is significantly higher than that of CK treatment. Among them, treatment 7 (500 mg/L Chitosan oligosaccharide solution + 10) 7 cfu/ml XY-13+700 times lentinan diluent) has the highest transpiration rate, net photosynthetic rate and stomatal conductance, which are higher than those of the treatment 1 by 60.0%, 68.5% and 28.6%. And the effect of the treatment 7 is better than that of the single lentinan treatment, the chitosan oligosaccharide solution and the bacillus amyloliquefaciens compound treatment.
TABLE 11 influence of Bacillus amyloliquefaciens, chitosanoligosaccharide, and lentinan compounding on the chemistry of the substrate
As can be seen from Table 11, the organic matter, the quick-acting nitrogen, the quick-acting phosphorus and the total nitrogen of the lentinan with different concentrations are all obviously higher than those of the blank control treatment, wherein the 700 times of lentinan diluent treatment is the best. Substrate chemistry for treatment 5 in addition to rapid onset potassiumThe properties were significantly higher than treatment 1. The chemical property indexes of the matrixes in the compounding treatment of the treatment 5 and the lentinan are obviously higher than those in the treatment 1, wherein the treatment 7 (500 mg/L chitosan oligosaccharide solution + 10) 7 cfu/ml XY-13+700 times lentinan diluent) has the highest content of organic matter, quick-acting nitrogen, quick-acting potassium and quick-acting phosphorus, which is 32.2%, 294.1%, 67.9% and 38.0% higher than that of 1. Treatment 6 (500 mg/L Chitosan oligosaccharide solution + 10) 7 cfu/ml XY-13+400 times lentinan diluent) has the highest total nitrogen content, which is 13.2 percent higher than that of 1.
As can be seen from tables 1 to 11, the chitosan oligosaccharide treatment can enhance the activity index of plant cells, promote the growth of roots and stems and leaves, increase the chlorophyll content and improve the photosynthesis of crops; compared with the combination of single chitosan oligosaccharide, lentinan, chitosan oligosaccharide and bacillus amyloliquefaciens, the compound composition has the advantages that the effect of promoting the growth of cucumber seedlings is remarkable, the cucumber seedlings can absorb nutrients and water and grow robustly, and the compound composition shows an obvious synergistic effect. Meanwhile, the compound composition has a more remarkable effect of improving the nutrients of the matrix soil.
Example 4 influence of the Chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan compounded composition on the low-temperature resistance of cucumber seedlings
The test uses two-leaf and one-heart Dell LD-1 cucumber seedlings as test materials, the water is uniformly irrigated according to the growth requirements of crops in the seedling stage, and 1 liter of water is irrigated once by one hole tray (72 holes). Culturing cucumber seedling in light culture box in normal temperature and light environment, irrigating root with clear water as control, and adding chitosan oligosaccharide at a concentration of 500mg/L +10 7 The cucumber seedlings are pretreated by the mixed solution of bacillus amyloliquefaciens and 700 times of lentinan for three days. And performing low-temperature stress treatment on the fourth day, and sampling to determine indexes such as growth vigor, antioxidant enzyme activity and the like until the tenth day. 3 different temperature combinations were set up in the experiment: the day and night temperature of the low-temperature treatment is controlled to be 20 ℃/12 ℃, 16 ℃/8 ℃ and the illumination time and intensity are unified by taking the day and night temperature of 28 ℃/18 ℃ as a reference. 0.5g of fresh leaves of different treated cucumber seedlings are weighed, and the content of MDA is determined by using a thiobarbituric acid method. Measurement of fresh sample size using the Nitro Blue Tetrazolium (NBT) methodSOD activity of Chinese cabbage leaves, POD activity is determined by guaiacol method, and CAT activity is determined by ultraviolet spectrophotometry.
Treatment 1: blank control, water only, day and night temperature: 28 ℃/18 DEG C
And (3) treatment 2:500mg/L Chitosan oligosaccharide solution +10 7 Bacillus amyloliquefaciens culture solution and 700 times lentinan diluent
And (3) treatment: blank control, water only, day and night temperature: 20 ℃/12 DEG C
And (4) treatment: 500mg/L Chitosan oligosaccharide solution +10 7 Bacillus amyloliquefaciens culture solution and 700 times lentinan diluent
And (4) treatment 5: blank control, water only, day and night temperature: 16 ℃/8 DEG C
And (6) treatment: 500mg/L Chitosan oligosaccharide solution +10 7 Bacillus amyloliquefaciens culture solution and 700 times lentinan diluent
As can be seen from FIG. 4, the plant height of cucumber seedlings decreased significantly with decreasing temperature. Under the same temperature environment, the plant height and the strong seedling index of the blank control treatment and the compound composition treatment have no obvious difference, but when the day and night temperature is 28 ℃/18 ℃, the stem thickness of the compound composition treatment is obviously higher than 4.4% of that of the blank control treatment, and when the day and night temperature is 20 ℃/12 ℃, the chlorophyll content of the compound composition treatment is obviously higher than 24.7% of that of the blank control treatment.
As can be seen from fig. 5, the dry fresh weight of the overground part and the underground part of the cucumber seedling tends to decrease with the decrease of the temperature. Under the same temperature environment, the dry fresh weight of the underground part and the dry fresh weight of the underground part between the blank control treatment and the compound composition treatment have no obvious difference, and when the day and night temperature is 20 ℃/12 ℃, the dry weight of the underground part treated by the compound composition is obviously higher than that of the blank control treatment by 27.2 percent. Overall, temperature changes have the greatest effect on the fresh weight of the underground.
As can be seen from FIG. 6, in different temperature environments, the activities of SOD, POD and CAT in the treatment of the compound composition were equal to or greater than that in the blank control treatment at each time, and the content of MDA was opposite to that in the blank control treatment. Under normal temperature and low temperature treatment, the enzyme activity and MDA content change trend of the compound composition treatment in each period is similar to that of the blank control treatment, the enzyme activity index of the compound composition treatment is higher than that of the blank control treatment, and the malondialdehyde content is lower than that of the blank control treatment. Under sub-low temperature treatment, namely day and night temperature of 20 ℃/12 ℃, POD and SOD activity treated by the compound composition are reduced within 2h and then greatly increased, and reach peak value within 8h, and POD and SOD activity treated by blank control firstly reduces and then slightly increases and then reduces after low temperature stress treatment. POD and SOD activities of T2 treatment at 8h after the treatment are obviously higher than those of blank control treatment of 38.3 percent and 77.2 percent. (ii) a CAT activity decreased at 2h and subsequently increased for both treatments 3 and 4, but treatment 4 increased faster and peaked higher than treatment 3; treatment 4 reached a peak MDA level at 2h, followed by a decrease and then a small increase, but treatment 3 reached a peak at 4h, followed by a decrease, with treatment 4 showing significantly lower MDA accumulation than treatment 3.
Low temperature stress is a major factor limiting the production of vegetables in a facility. The cucumber is warm in nature and not cold-resistant, the growth of the cucumber can be obviously inhibited by low-temperature stress, particularly the plant height, stem thickness and biomass accumulation of the cucumber are limited, and the chlorophyll content and photosynthesis of the cucumber are influenced. The application discovers that normal growth of cucumber seedlings is influenced under a low-temperature environment through a low-temperature stress treatment test on the cucumber seedlings, and the influence is larger when the temperature is lower. And the root irrigation of the compound composition can remarkably relieve the indexes of chlorophyll, underground dry weight and the like of the cucumber seedling. Secondly, low-temperature treatment affects both the activity of antioxidant enzymes and the content of malondialdehyde in leaves of the cucumber seedlings, and after the cucumber seedlings are stressed at low temperature, the enzyme activity of the cucumber seedlings is reduced, then the enzyme activity is increased, and then the enzyme activity is slowly reduced; the change trend of malondialdehyde is opposite to that of malondialdehyde. The activity of the cucumber seedling enzyme treated by adding the compound composition is obviously higher than that of the cucumber seedling enzyme treated by the blank control treatment, and the content of malondialdehyde of the cucumber seedling enzyme is obviously lower than that of the cucumber seedling enzyme treated by the blank control treatment. The compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan disclosed by the invention can improve the low-temperature resistance of cucumber seedlings by root irrigation, and has important significance for cultivating strong cucumber seedlings and safely overwintering the cucumber seedlings.
Example 5 Effect of Chitosan oligosaccharides on the Activity of Bacillus amyloliquefaciens
Inoculating a single colony of the bacillus amyloliquefaciens into a 50mL triangular flask containing 10mL LB broth, and culturing at 30 ℃ for 24 hours at 180r/min to obtain a seed solution; 1mL of the seed solution was taken in a 250mL Erlenmeyer flask containing 50mL of LB broth, and 500mg/L of a chitosan oligosaccharide solution was added in an amount of 1%, 2%, 3% and 4% of the inoculum size (50 mL), incubated at 180r/min at 30 ℃ and OD600 was measured at 0, 4, 8, 12, 16, 20, 24, 48 and 72 hours, respectively.
Treatment 1: inoculation of Strain treatment alone
And (3) treatment 2: strain +0.5ml 500mg/L chitooligosaccharide solution
And (3) treatment: strain +1ml 500mg/L chitosan oligosaccharide solution
And (4) treatment: strain +1.5ml 500mg/L chitooligosaccharide solution
And (4) treatment 5: strain +2ml 500mg/L chitosan oligosaccharide solution
Referring to FIG. 7, the results of the chitosan oligosaccharide addition on the activity of Bacillus amyloliquefaciens show that the chitosan oligosaccharide has an effect of promoting the proliferation of bacteria with the increase of time after 8 hours.
Claims (3)
1. A compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan is characterized in that: the compound composition is in a liquid form, wherein the concentration of the chitosan oligosaccharide is 100-700mg/L, and the viable count of the bacillus amyloliquefaciens is 10 5 -10 8 cfu/mL, wherein the lentinan is 400-1000 times of lentinan diluent.
2. The compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan as claimed in claim 1, wherein: the concentration of the chitosan oligosaccharide is 500mg/L, and the viable count of the bacillus amyloliquefaciens is 10 7 cfu/mL, wherein the lentinan is 700 times of lentinan diluent.
3. Use of a complex composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan as claimed in any one of claims 1 to 2 for promoting the growth of cucumber seedlings.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211572787.XA CN115812719B (en) | 2022-12-08 | 2022-12-08 | Compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application of compound composition in promoting cucumber seedling growth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211572787.XA CN115812719B (en) | 2022-12-08 | 2022-12-08 | Compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application of compound composition in promoting cucumber seedling growth |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115812719A true CN115812719A (en) | 2023-03-21 |
| CN115812719B CN115812719B (en) | 2024-03-01 |
Family
ID=85545473
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211572787.XA Active CN115812719B (en) | 2022-12-08 | 2022-12-08 | Compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application of compound composition in promoting cucumber seedling growth |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115812719B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103190404A (en) * | 2013-04-02 | 2013-07-10 | 中国农业科学院植物保护研究所 | Composition for preventing and treating plant nematode and virus diseases, and application thereof |
| CN104531559A (en) * | 2014-12-08 | 2015-04-22 | 山西省农业科学院生物技术研究中心 | Bacillusamyloliquefaciens subsp Lh-1 and application thereof |
| CN107006519A (en) * | 2017-04-19 | 2017-08-04 | 陕西康禾立丰生物科技药业有限公司 | A kind of efficient bactericidal composite containing bacillus amyloliquefaciens |
| CN113303340A (en) * | 2021-05-21 | 2021-08-27 | 宁夏大学 | Composition of biocontrol bacteria and fish protein and application of composition in cucumber cultivation |
| CN114908021A (en) * | 2022-06-13 | 2022-08-16 | 中国农业科学院蔬菜花卉研究所 | Bacillus amyloliquefaciens and application thereof in preventing and treating cucumber corynespora leaf spot |
| CN115261282A (en) * | 2022-08-26 | 2022-11-01 | 甘肃省科学院生物研究所 | Bacillus amyloliquefaciens and application thereof in cucumber disease prevention and control |
-
2022
- 2022-12-08 CN CN202211572787.XA patent/CN115812719B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103190404A (en) * | 2013-04-02 | 2013-07-10 | 中国农业科学院植物保护研究所 | Composition for preventing and treating plant nematode and virus diseases, and application thereof |
| CN104531559A (en) * | 2014-12-08 | 2015-04-22 | 山西省农业科学院生物技术研究中心 | Bacillusamyloliquefaciens subsp Lh-1 and application thereof |
| CN107006519A (en) * | 2017-04-19 | 2017-08-04 | 陕西康禾立丰生物科技药业有限公司 | A kind of efficient bactericidal composite containing bacillus amyloliquefaciens |
| CN113303340A (en) * | 2021-05-21 | 2021-08-27 | 宁夏大学 | Composition of biocontrol bacteria and fish protein and application of composition in cucumber cultivation |
| CN114908021A (en) * | 2022-06-13 | 2022-08-16 | 中国农业科学院蔬菜花卉研究所 | Bacillus amyloliquefaciens and application thereof in preventing and treating cucumber corynespora leaf spot |
| CN115261282A (en) * | 2022-08-26 | 2022-11-01 | 甘肃省科学院生物研究所 | Bacillus amyloliquefaciens and application thereof in cucumber disease prevention and control |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115812719B (en) | 2024-03-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20220322678A1 (en) | Chlorella-based composition, and methods of its preparation and application to plants | |
| CN105331567B (en) | A kind of bacillus amyloliquefaciens and promoting growth of plants preparation and application | |
| CN112538431B (en) | Chlorella pyrenoidosa, preparation method of biological environment restoration liquid and application thereof | |
| CN111670769B (en) | Method for improving stress resistance of rice | |
| CN112746024A (en) | Trichoderma composite microbial agent and bio-organic fertilizer prepared from same | |
| CN113999837B (en) | Agricultural microbial agent and preparation process thereof | |
| CN115536446A (en) | Fertilizer for promoting plant growth and application thereof | |
| CN112715290A (en) | Production method for obtaining high sulforaphane broccoli | |
| CN116814496A (en) | Bacteria capable of dissolving phosphorus efficiently and promoting plant growth and application thereof | |
| CN115812719B (en) | Compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application of compound composition in promoting cucumber seedling growth | |
| CN106929426B (en) | Compound microbial agent for potatoes and preparation method thereof | |
| CN111763631B (en) | Pheretima aspergillum and its application | |
| CN114793828A (en) | Tobacco seedling culture medium and preparation and use methods thereof | |
| CN112624840A (en) | Biological pesticide fertilizer containing bacillus methylotrophicus | |
| CN111670782A (en) | Composite microbial cultivation substrate and application thereof | |
| CN106922739B (en) | Drought-resistant biological source stimulant, crop fertilizer and crop irrigation method | |
| CN115011488B (en) | Penicillium purpureum C1-GP and application thereof in cultivation of blueberry mycorrhizal seedlings | |
| CN115843806A (en) | Compound composition of seaweed oligosaccharide, bacillus subtilis and lentinan and application of compound composition in promoting growth of cucumber seedlings | |
| CN116458357B (en) | Method for promoting growth of fruit trees by rhizosphere soil microorganisms | |
| CN113678700B (en) | Nanmu rice weed control method | |
| CN112501082B (en) | Compound microbial liquid and application thereof in hydroponic cucumber | |
| CN115428798B (en) | Seed treatment agent, preparation method and application thereof | |
| CN116083261B (en) | Preparation and application of special carbon-based microbial fertilizer for apple cultivation | |
| CN117821286B (en) | Microbial composite microbial agent for saline-alkali soil improvement and preparation method thereof | |
| CN113207348B (en) | Method for improving low-temperature emergence rate and antioxidase activity of corn seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |