CN103190404A - Composition for preventing and treating plant nematode and virus diseases, and application thereof - Google Patents
Composition for preventing and treating plant nematode and virus diseases, and application thereof Download PDFInfo
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Abstract
The invention discloses a composition for preventing and treating plant nematode and virus diseases, and an application of the composition, belonging to the technical field of biopesticide. The composition takes chitosan oligosaccharide and lentinan as active ingredients and can be made into liquid or effervescent tablets. The composition can be used for separately and simultaneously preventing and treating the plant nematode and the virus diseases of plants, and has good using effect on pumpkins, winter squashes, pepper, alfalfa, lily, strawberries and gingers, thus effectively reducing the crop diseases and pest and improving the yield and the quality of crops.
Description
Technical field
The invention belongs to biological pesticide technical field, be specifically related to a kind of composition and application thereof that prevents and treats Plant nematode and virus disease.
Background technology
Root-knot nematode (Meloidogyne spp.) is to parasitize crop root, the class phytopathy original that the serious harm world agriculture is produced.Its host plant is above 3000 kinds.Disease can cause crop failure 10%-20%, can reach more than 75% when serious.Plant hazard is only second to fungal disease, causes about 1,000 hundred million dollars loss every year.Nematode not only directly endangers host plant, can also spread germs and virus.Chemical control occupies critical role preventing and treating of root-knot nematode in the process, but the chemical nematode killing agent that uses belongs to single agent or the complex preparation of organic phosphor or carbamates more at present, and belong to high-toxic pesticide (The Ministry of Agriculture of the People's Republic of China, MOA agriculture chemical examination institution more, 2003), there are problems such as toxicity is big, environmental pollution is serious, nematode easily develops immunity to drugs.Along with enhancing and the drug-fast increase of nematode of public's environmental consciousness, the application of chemical nematocide is restricted day by day.Therefore, need have better effect and the environmentally friendly pesticide species of preventing and treating nematodiasis.
Viroses of plant proportion in plant disease is only second to fungal disease, and the annual agricultural product that caused by virus disease are with a toll of 10% according to statistics, and whole world every year, only harm of tobacco mosaic virus disease just caused 100,000,000 dollars economic loss.Along with the development of industrialized agriculture, the harm of virus disease on economic crops is more and more heavy at present.Plant virus quantity is many, harm is wide, economic loss big and be difficult to control, and the pharmacy control efficacy of preventing and treating virus disease in the production is difficult to effectively control the harm of virus disease generally at 30%-60% at present, needs the new medicament of preventing and treating virus disease.
The main component of mushroom is polysaccharose and fatty acid, also contains ergosterol and the fungisterol of a large amount of changed into vitamin Ds.Mushroom has had strong inhibitory effects to cancer cell.Lentinan (Lentinan) is the effective active composition that extracts from the quality xianggu fruit body, is a kind of host immunity reinforcing agent, have antiviral, regulate the plant immune function and stimulate functions such as interferon formation.By the passivation virus activity, effectively break virus gene and virocyte suppress virus replication.
The activity of Chitosan poly oligosaccharide energy activated plant enzyme, the inducing plant system obtains disease resistance.Changing the main mode of action one of material is the interior chitinase of activated plant body, thereby improves the defence function of plant self, promotes the formation of plant callus, improves plant to the resistance of nematode.The 2nd, induce the generation phytoalexin, as chitinase, chitinase can decompose the body wall that destroys nematode, thereby kills nematode.The 3rd, can enter in the cell of nematode and pathogen, hinder pathogen transcribing from DNA to RNA, thereby hindered growth or the propagation of nematode.The 4th, can make the enzyme system that nematode is killed in the certain micro-organisms secretion in the soil, become the effective means of control nematode infringement plant.When the oligosaccharides degree of polymerization was 6-8, chitosan oligosaccharide can excite the immune system of plant corpus effectively, the generation that produces chitin enzyme, dextranase, is rich in hydroxyproline glycoprotein etc.These enzymes are direct material or prerequisite materials that crop is resisted disease and root nematode.The control molecular weight improves absorption rate to greatest extent between 1500-2000.
Summary of the invention
The object of the present invention is to provide a kind of environmental friendliness, effect is obvious, is mixed and reasonably prevents and treats the composition of Plant nematode and virus disease.
The present invention also aims to provide the application of above-mentioned composition in control Plant nematode and virus disease.
A kind of composition of preventing and treating Plant nematode and virus disease, described composition is active component with Chitosan poly oligosaccharide and lentinan.
The weight ratio of described Chitosan poly oligosaccharide and lentinan is 1: (0.01-100).
Preferably, the weight ratio of described Chitosan poly oligosaccharide and lentinan is 1: (0.1-50).
Preferred, the weight ratio of described Chitosan poly oligosaccharide and lentinan is 1: (1-10).
The formulation of the composition of described control Plant nematode and virus disease is aqua or effervescent tablet.
Aqua component and the percentage composition of described control Plant nematode and virus disease are: active component 1-60wt%, and antifreezing agent 1-10wt%, stabilizing agent 5-10wt%, surplus is water;
It is described that to freeze agent be in ethylene glycol, propane diols, the glycerine one or more; Described stabilizing agent is urea or ethanol; Described water is deionized water.
Component and the percentage composition of the effervescent tablet of described control Plant nematode and virus disease are: active component 1-60wt%, effervescent agent 1-20%, binding agent 3-10wt%, disintegrant 0.5-30wt%, wetting agent 0.5-20wt%, dispersant 0.5-30wt%, filler 0.5-20wt%;
Described effervescent agent is one or more in sodium carbonate, sodium bicarbonate, potash, the citric acid; Binding agent is one or more in industrial starch, methylcellulose, carboxymethyl cellulose, the polyvinylpyrrolidone; Disintegrant is one or more in microcrystalline cellulose, Ac-Di-Sol, sodium carboxymethylcellulose, crospolyvinylpyrrolidone, the hydroxypropyl cellulose; Wetting agent is one or more in bay alcohol radical sodium sulphate, neopelex, the lauryl sodium sulfate; Dispersant is how sodium sulfonate of sodium lignin sulfonate or dibutyl; Filler is one or more in lactose, calcium sulphate, sucrose, talcum powder, silica gel, dolomol, the precipitated calcium carbonate.
The application of the composition of described control Plant nematode and virus disease in control Plant nematode and plant virus.
Described Plant nematode is one or more in pepper root-knot nematode, strawberry root-knot nematode, race tie lines worm, clover root-knot nematode, the pumpkin root-knot nematode.
The described viroses of plant are pumpkin virus disease [pumpkin mosaic virus Squash mosaic virus; SqMV]; winter squash mosaic disease [pumpkin mosaic virus Squash mosaic virus is called for short SqMV]; pepper virus disease [cucumber mosaic virus (cumber mosaic virus; CMV)]; clover virus disease [alfalfa mosaic virus (Alfalfamosaicvirus; AlMV)]; lily disease viral disease [lily mosaic virus (Lily mosaic virus)]; downright bad pinta [lily cryptovirus (Lily symtomless virus) and cucumber mosaic virus (cumber mosaic virus, CMV)]; ring spot [lily ring spot virus (Lily ring spot virus)]; in the rosette [(Lily rosettle virus)] one or more.
The composition of described control Plant nematode and virus disease mainly uses pumpkin, winter squash, pepper, clover, lily, strawberry and ginger, mainly uses behind the dilute with water before virus takes place, and can make these plants not infect virus in breeding time.
Beneficial effect of the present invention: composition of the present invention can be respectively with prevent and treat Plant nematode and the viroses of plant simultaneously, result of use is good on pumpkin, winter squash, pepper, clover, lily, strawberry and ginger, effectively reduce the generation of diseases and pests of agronomic crop, increased output and the quality of crops.
Embodiment
The present invention will be further described below in conjunction with specific embodiment.
Embodiment 1
The composition of present embodiment comprises following component: Chitosan poly oligosaccharide 7 grams, lentinan 7 grams, ethylene glycol 5 grams, glycerine 5 grams, urea 5 grams, water 71 grams.
Its preparation process is as follows:
With Chitosan poly oligosaccharide, lentinan, ethylene glycol, glycerine, urea adds in the configuration still successively and water mixes, and stirs, and namely makes 14% aqua.
Embodiment 2
The composition of present embodiment comprises following component: Chitosan poly oligosaccharide 0.01 gram, lentinan 1 gram, propane diols 6 grams, urea 6.99 grams, water 86 grams.
Its preparation process is as follows:
With Chitosan poly oligosaccharide, lentinan, propane diols, urea adds in the configuration still successively and water mixes, and stirs, and namely makes 1.01% aqua.
Embodiment 3
The composition of present embodiment comprises following component: Chitosan poly oligosaccharide 30 grams, lentinan 1 gram, propane diols 1 gram, glycerine 2 grams, ethanol 10 grams, water 56 grams.
Its preparation process is as follows:
With Chitosan poly oligosaccharide, lentinan, propane diols, glycerine, ethanol adds in the configuration still successively and water mixes, and stirs, and namely makes 31% aqua.
Embodiment 4
The composition pesticide of present embodiment comprises following component: Chitosan poly oligosaccharide 32 grams, lentinan 28 grams, ethylene glycol 1 gram, urea 3 grams, water 36 grams.
Its preparation process is as follows:
With Chitosan poly oligosaccharide, lentinan, ethylene glycol, urea adds in the configuration still successively and water mixes, and stirs, and namely makes 60% aqua.
Embodiment 5
The composition of present embodiment comprises following component: Chitosan poly oligosaccharide 3 grams, lentinan 2 grams, propane diols 10 grams, ethanol 5 grams, water 80 grams.
Its preparation process is as follows:
With Chitosan poly oligosaccharide, lentinan, propane diols, ethanol adds in the configuration still successively and water mixes, and stirs, and namely makes 5% aqua.
Embodiment 6
The composition of present embodiment comprises following component: Chitosan poly oligosaccharide 15 grams, lentinan 1 gram, ethylene glycol 2 grams, urea 2 grams, water 80 grams.
Its preparation process is as follows:
With Chitosan poly oligosaccharide, lentinan, ethylene glycol, urea adds in the configuration still successively and water mixes, and stirs, and namely makes 16% aqua.
Embodiment 7
The composition of present embodiment comprises following component: Chitosan poly oligosaccharide 3 grams, lentinan 3 grams, sodium bicarbonate 20 grams, industrial starch 3 grams, microcrystalline cellulose 13 grams, sodium carboxymethylcellulose 15 grams, lauryl sodium sulfate 5 grams, sodium lignin sulfonate 30 grams, talcum powder 7 grams, precipitated calcium carbonate 1 gram.
The following institute of its preparation process is not:
Each drying materials is levigate, fully mix, make it to decompose evenly, direct tablet compressing namely makes 6% effervescent tablet.
Embodiment 8
The composition pesticide of present embodiment comprises following component: Chitosan poly oligosaccharide 0.01 gram, lentinan 1 gram, sodium bicarbonate 8.99 grams, citric acid 10 grams, industrial starch 10 grams, sodium carboxymethylcellulose 30 grams, lauryl sodium sulfate 20 grams, sodium lignin sulfonate 0.5 gram, lactose 4.5 grams, precipitated calcium carbonate 15 grams;
Its preparation process is as follows:
Each drying materials is levigate, fully mix, make it to decompose evenly, direct tablet compressing namely makes 1.01% effervescent tablet.
Embodiment 9
The composition pesticide of present embodiment comprises following component: Chitosan poly oligosaccharide 30 grams, lentinan 0.6 gram, sodium bicarbonate 10 grams, citric acid 8.4 grams, methylcellulose 10 grams, hydroxypropyl cellulose 0.5 gram, neopelex 10 grams, how sodium sulfonate 30 restrains dibutyl, silica gel 0.5 gram;
Its preparation process is as follows:
Each drying materials is levigate, fully mix, make it to decompose evenly, direct tablet compressing namely makes 30.6% effervescent tablet.
Embodiment 10
The composition pesticide of present embodiment comprises following component: Chitosan poly oligosaccharide 32 grams, lentinan 28 grams, citric acid 4 grams, industrial starch 3 grams, microcrystalline cellulose 30 grams, lauryl sodium sulfate 0.3 gram, neopelex 0.2 gram, sodium lignin sulfonate 0.4 gram, how sodium sulfonate 0.1 restrains dibutyl, lactose 2 grams.
Its preparation process is as follows:
Each drying materials is levigate, fully mix, make it to decompose evenly, direct tablet compressing namely makes 60% effervescent tablet.
Embodiment 11
The composition pesticide of present embodiment comprises following component: Chitosan poly oligosaccharide 15 grams, lentinan 1 gram, sodium carbonate 6 grams, citric acid 6 grams, polyvinylpyrrolidone 10 grams, microcrystalline cellulose 22 grams, neopelex 18 grams, how sodium sulfonate 2 restrains dibutyl, dolomol 1 gram, silica gel 0.5 gram, precipitated calcium carbonate 17 grams.
Its preparation process is as follows:
Each drying materials is levigate, fully mix, make it to decompose evenly, direct tablet compressing namely makes 16% effervescent tablet.
Embodiment 12
The composition pesticide of present embodiment comprises following component: Chitosan poly oligosaccharide 3 grams, lentinan 2 grams, citric acid 20 grams, industrial starch 4 grams, polyvinylpyrrolidone 6 grams, microcrystalline cellulose 21 grams, neopelex 15 grams, how sodium sulfonate 25 restrains dibutyl, silica gel 4 grams.
Its preparation process is as follows:
Each drying materials is levigate, fully mix, make it to decompose evenly, direct tablet compressing namely makes 5% effervescent tablet.
Experimental example 13 aquas and effervescent tablet preparation control pumpkin root-knot nematode and pumpkin mosaic virus (SqMV) field control effectiveness test effect
Table 1
Experimental example 14 aquas and effervescent tablet preparation control clover root-knot nematode and alfalfa mosaic virus (A1MV) field control effectiveness test effect
Table 2
By table 1 and table 2 as can be seen, the aqua of embodiment 1-12 and effervescent tablet preparation be effectively preventing pumpkin root-knot nematode and pumpkin mosaic virus simultaneously, control clover root-knot nematode and alfalfa mosaic virus.
Claims (10)
1. a composition of preventing and treating Plant nematode and virus disease is characterized in that, described composition is active component with Chitosan poly oligosaccharide and lentinan.
2. according to the described a kind of composition of preventing and treating Plant nematode and virus disease of claim 1, it is characterized in that the weight ratio of described Chitosan poly oligosaccharide and lentinan is 1: (0.01-100).
3. according to the described a kind of composition of preventing and treating Plant nematode and virus disease of claim 2, it is characterized in that the weight ratio of described Chitosan poly oligosaccharide and lentinan is 1: (0.1-50).
4. according to the described a kind of composition of preventing and treating Plant nematode and virus disease of claim 3, it is characterized in that the weight ratio of described Chitosan poly oligosaccharide and lentinan is 1: (1-10).
5. according to the composition of each described control Plant nematode of claim 1-4 and virus disease, it is characterized in that the formulation of the composition of described control Plant nematode and virus disease is aqua or effervescent tablet.
6. according to the composition of the described control Plant nematode of claim 5 and virus disease, it is characterized in that aqua component and the percentage composition of described control Plant nematode and virus disease are: active component 1-60wt%, antifreezing agent 1-10wt%, stabilizing agent 5-10wt%, surplus is water;
It is described that to freeze agent be in ethylene glycol, propane diols, the glycerine one or more; Described stabilizing agent is urea or ethanol; Described water is deionized water.
7. according to the composition of the described control Plant nematode of claim 5 and virus disease, it is characterized in that, component and the percentage composition of the effervescent tablet of described control Plant nematode and virus disease are: active component 1-60wt%, effervescent agent 1-20%, binding agent 3-10wt%, disintegrant 0.5-30wt%, wetting agent 0.5-20wt%, dispersant 0.5-30wt%, filler 0.5-20wt%;
Described effervescent agent is one or more in sodium carbonate, sodium bicarbonate, potash, the citric acid; Binding agent is one or more in industrial starch, methylcellulose, carboxymethyl cellulose, the polyvinylpyrrolidone; Disintegrant is one or more in microcrystalline cellulose, Ac-Di-Sol, sodium carboxymethylcellulose, crospolyvinylpyrrolidone, the hydroxypropyl cellulose; Wetting agent is one or more in bay alcohol radical sodium sulphate, neopelex, the lauryl sodium sulfate; Dispersant is how sodium sulfonate of sodium lignin sulfonate or dibutyl; Filler is one or more in lactose, calcium sulphate, sucrose, talcum powder, silica gel, dolomol, the precipitated calcium carbonate.
8. the application of the composition of each described control Plant nematode of claim 1-7 and virus disease in control Plant nematode and plant virus.
9. the application of the composition of described control Plant nematode and virus disease in control Plant nematode and the viroses of plant according to Claim 8, it is characterized in that described Plant nematode is one or more in pepper root-knot nematode, strawberry root-knot nematode, race tie lines worm, clover root-knot nematode, the pumpkin root-knot nematode.
10. the application of the composition of described control Plant nematode and virus disease in control Plant nematode and the viroses of plant according to Claim 8, it is characterized in that the described viroses of plant are one or more in pumpkin virus disease, winter squash mosaic disease, pepper virus disease, clover virus disease, lily disease viral disease, downright bad pinta, cucumber mosaic virus, ring spot, the rosette.
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| CN103548828A (en) * | 2013-11-08 | 2014-02-05 | 成都新朝阳作物科学有限公司 | Melon and fruit immunizing agent and application thereof |
| CN103636637A (en) * | 2013-12-04 | 2014-03-19 | 郭红甫 | Pesticide composition for preventing and treating crop root nematodosis by foliage spray |
| CN103719088A (en) * | 2013-12-25 | 2014-04-16 | 广西南宁碧湾园林工程有限公司 | Biological source pesticide composition for preventing and treating plant virus and application thereof |
| CN103782888A (en) * | 2014-03-05 | 2014-05-14 | 北京农业生物技术研究中心 | Seedling growth matrix capable of reducing plant virus harm and preparation method and application thereof |
| CN104336071A (en) * | 2013-07-31 | 2015-02-11 | 陕西美邦农药有限公司 | An antibacterial yield-increasing composition containing lentinan |
| CN104621169A (en) * | 2015-03-04 | 2015-05-20 | 山东科大创业生物有限公司 | Compound preparation for killing nematodes |
| CN106719855A (en) * | 2016-12-22 | 2017-05-31 | 钟祥品红植物免疫科技有限公司 | A kind of viroses of plant immunizing agent and its preparation method and application |
| CN107318844A (en) * | 2017-08-01 | 2017-11-07 | 山东圣鹏科技股份有限公司 | A kind of anti-phytoplasma composition containing lentinan |
| CN109463398A (en) * | 2018-12-27 | 2019-03-15 | 成都新朝阳作物科学有限公司 | Chitin derivativ composition and preparation method thereof for preventing soil-borne disease |
| CN115812719A (en) * | 2022-12-08 | 2023-03-21 | 宁夏大学 | Compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application of compound composition in promoting growth of cucumber seedlings |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104336071A (en) * | 2013-07-31 | 2015-02-11 | 陕西美邦农药有限公司 | An antibacterial yield-increasing composition containing lentinan |
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| CN103636637A (en) * | 2013-12-04 | 2014-03-19 | 郭红甫 | Pesticide composition for preventing and treating crop root nematodosis by foliage spray |
| CN103636637B (en) * | 2013-12-04 | 2015-10-28 | 郭红甫 | A kind of composition pesticide of foliage-spray control crop root nematodosis |
| CN103719088B (en) * | 2013-12-25 | 2015-11-18 | 广西南宁碧湾园林工程有限公司 | A kind of biogenic pesticide composition and application thereof preventing and treating the viroses of plant |
| CN103719088A (en) * | 2013-12-25 | 2014-04-16 | 广西南宁碧湾园林工程有限公司 | Biological source pesticide composition for preventing and treating plant virus and application thereof |
| CN103782888A (en) * | 2014-03-05 | 2014-05-14 | 北京农业生物技术研究中心 | Seedling growth matrix capable of reducing plant virus harm and preparation method and application thereof |
| CN104621169A (en) * | 2015-03-04 | 2015-05-20 | 山东科大创业生物有限公司 | Compound preparation for killing nematodes |
| CN104621169B (en) * | 2015-03-04 | 2017-01-04 | 山东科大创业生物有限公司 | A kind of nematicide combination preparation |
| CN106719855A (en) * | 2016-12-22 | 2017-05-31 | 钟祥品红植物免疫科技有限公司 | A kind of viroses of plant immunizing agent and its preparation method and application |
| CN107318844A (en) * | 2017-08-01 | 2017-11-07 | 山东圣鹏科技股份有限公司 | A kind of anti-phytoplasma composition containing lentinan |
| CN109463398A (en) * | 2018-12-27 | 2019-03-15 | 成都新朝阳作物科学有限公司 | Chitin derivativ composition and preparation method thereof for preventing soil-borne disease |
| CN115812719A (en) * | 2022-12-08 | 2023-03-21 | 宁夏大学 | Compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application of compound composition in promoting growth of cucumber seedlings |
| CN115812719B (en) * | 2022-12-08 | 2024-03-01 | 宁夏大学 | Compound composition of chitosan oligosaccharide, bacillus amyloliquefaciens and lentinan and application of compound composition in promoting cucumber seedling growth |
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Inventor after: Mei Xiangdong Inventor after: Ning Jun Inventor after: Zhang Tao Inventor after: Xie Ruliang Inventor after: Guo Zheng Inventor before: Mei Xiangdong Inventor before: Ning Jun Inventor before: Zhang Tao Inventor before: Xie Ruliang |
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Effective date of registration: 20151117 Address after: 541004, E building, block 4, innovation building, information industry park, national hi tech Zone, Guilin, the Guangxi Zhuang Autonomous Region Patentee after: Guilin Jiqi Biochemical Co., Ltd. Address before: 100193 Beijing Old Summer Palace West Road, Haidian District, No. 2 Patentee before: Institute of Plant Protection, Chinese Academy of Agricultural Sciences |
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Addressee: Mei Xiangdong Document name: Notification of Passing Examination on Formalities |