CN116458357B - Method for promoting growth of fruit trees by rhizosphere soil microorganisms - Google Patents
Method for promoting growth of fruit trees by rhizosphere soil microorganisms Download PDFInfo
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- CN116458357B CN116458357B CN202310633632.0A CN202310633632A CN116458357B CN 116458357 B CN116458357 B CN 116458357B CN 202310633632 A CN202310633632 A CN 202310633632A CN 116458357 B CN116458357 B CN 116458357B
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- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 51
- 230000012010 growth Effects 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000002689 soil Substances 0.000 title claims abstract description 12
- 244000005700 microbiome Species 0.000 title claims abstract description 11
- 230000001737 promoting effect Effects 0.000 title claims abstract description 10
- 241000894006 Bacteria Species 0.000 claims abstract description 24
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 16
- 241000220324 Pyrus Species 0.000 claims abstract description 13
- 235000021016 apples Nutrition 0.000 claims abstract description 11
- 235000021017 pears Nutrition 0.000 claims abstract description 11
- 230000001580 bacterial effect Effects 0.000 claims description 58
- 239000007788 liquid Substances 0.000 claims description 54
- 241000976738 Bacillus aryabhattai Species 0.000 claims description 12
- 241000233866 Fungi Species 0.000 claims description 10
- 206010033546 Pallor Diseases 0.000 claims description 3
- 241000857964 Ileodictyon cibarium Species 0.000 claims description 2
- 241001536563 Panus Species 0.000 claims description 2
- 244000141359 Malus pumila Species 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 15
- 241000220225 Malus Species 0.000 abstract description 11
- 235000020234 walnut Nutrition 0.000 abstract description 11
- 235000009496 Juglans regia Nutrition 0.000 abstract description 9
- 241000167854 Bourreria succulenta Species 0.000 abstract description 2
- 235000003095 Vaccinium corymbosum Nutrition 0.000 abstract description 2
- 240000000851 Vaccinium corymbosum Species 0.000 abstract description 2
- 235000017537 Vaccinium myrtillus Nutrition 0.000 abstract description 2
- 235000021014 blueberries Nutrition 0.000 abstract description 2
- 235000019693 cherries Nutrition 0.000 abstract description 2
- 240000007049 Juglans regia Species 0.000 abstract 1
- 241000758789 Juglans Species 0.000 description 8
- 239000008223 sterile water Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000035882 stress Effects 0.000 description 5
- 238000012258 culturing Methods 0.000 description 4
- 239000003337 fertilizer Substances 0.000 description 4
- 208000000260 Warts Diseases 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 201000010153 skin papilloma Diseases 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 241000758791 Juglandaceae Species 0.000 description 2
- 241000228347 Monascus <ascomycete fungus> Species 0.000 description 2
- 235000014443 Pyrus communis Nutrition 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000036579 abiotic stress Effects 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012935 Averaging Methods 0.000 description 1
- 241001518729 Monilinia Species 0.000 description 1
- 235000010724 Wisteria floribunda Nutrition 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000004790 biotic stress Effects 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a method for promoting growth of fruit trees by rhizosphere soil microorganisms, which belongs to the field of promoting growth of fruit trees and comprises the step of applying pallidum, bacillus arvensis and basket-shaped yellow bacteria to the fruit trees. The method provided by the invention can improve the growth of apples, pears, blueberries and cherries, promote the growth of the apples, improve the single plant yield of the apples by 12.7%, and improve the single fruit weight by 9.6%; the single fruit weight of the pears is improved by 11.3 percent, and the quality of the pears is improved; the growth of walnut seedlings is improved by 20.7 percent.
Description
Technical Field
The invention relates to the field of fruit tree growth promotion, in particular to a method for promoting fruit tree growth by rhizosphere soil microorganisms.
Background
With the improvement of life quality, people pay more attention to the safety of foods, and in the production of crops, fertilizers take a quite important role. The chemical fertilizer can provide nutrients required by crops and plants for growth, but the defects of the chemical fertilizer are gradually exposed after years of practice, the inorganic matter content is increased suddenly, the organic matter is weakened, the microelements are unbalanced, the crop diseases and insect pests are serious day by day in the soil with excessive application of the chemical fertilizer, and the crops planted in the soil environment are unfavorable for human health.
In addition, in agricultural production, adverse environmental factors often pose serious threats to crop production, and these factors adverse to plant growth are collectively referred to as stress. Stress generally includes abiotic stress and biotic stress. Biological stress mainly comprises various diseases and insect pests, and abiotic stress comprises drought, high temperature, low temperature, high salt, toxic and harmful gas and the like. Whether the plant is in the biological stress or the non-biological stress, the growth index of the plant can be changed, the growth and development process of the plant can be influenced, the growth of the plant can be blocked, and the yield and quality of crops can be seriously influenced.
The beneficial effects of rhizosphere soil microorganisms on plants are mainly represented by improving nutrient absorption of plants, promoting plant growth, improving yield and the like, so that proper rhizosphere soil microorganisms are required to promote fruit tree growth and improve yield.
Disclosure of Invention
In order to solve the problems, the invention provides a method for promoting the growth of fruit trees by rhizosphere soil microorganisms.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a method for promoting growth of fruit trees by rhizosphere soil microorganisms, which comprises the step of applying pallidum, bacillus arvensis and basket-shaped bacteria to the fruit trees.
Preferably, the fruit tree comprises one or more of apples, pears and walnuts.
Preferably, the pallidum, the bacillus arvensis and the basket-shaped bacteria are applied to the fruit tree in the form of bacterial liquid.
Preferably, the bacterial content of the pallidum in the bacterial liquid is 1-2 multiplied by 10 10 CFU/mL；
The bacillus aryabhattai in the bacterial liquid has the bacterial content of 0.5-1.5x10 8 CFU/mL；
The fungus content of the basket-shaped fungus in the fungus liquid is 1.2-1.5x10 10 CFU/mL。
Preferably, the bacterial content of the pallor bacillus in the bacterial liquid is 1.5x10 10 CFU/mL；
The bacillus aryabhattai in the bacterial liquid has the bacterial content of 1 multiplied by 10 8 CFU/mL；
The fungus content of the yellow basket fungus in the fungus liquid is 1.3X10 10 CFU/mL。
Preferably, when the fruit tree is a fruiting tree, the amount of the fungus liquid applied to each fruit tree is 2-3L.
Preferably, when the fruit tree is a fruiting tree, the amount of the bacterial liquid applied per fruit tree is 2.5L.
Preferably, the bacterial liquid is applied to the root of a fruit tree, and is applied 1 time in spring every year.
Preferably, when the fruit trees are seedlings, the amount of the fungus liquid applied to each fruit tree is 5-10 mL.
Preferably, when the fruit tree is a seedling, the amount of the bacterial liquid applied to each fruit tree is 8mL.
The beneficial effects of the invention are as follows:
the method provided by the invention can improve the growth of apples, pears, blueberries and cherries, promote the growth of the apples, improve the single plant yield of the apples by 12.7%, and improve the single fruit weight by 9.6%; the single fruit weight of the pears is improved by 11.3 percent, and the quality of the pears is improved; the growth of walnut seedlings is improved by 20.7 percent.
Detailed Description
The invention provides a method for promoting growth of fruit trees by rhizosphere soil microorganisms, which comprises the step of applying pallidum, bacillus arvensis and basket-shaped bacteria to the fruit trees.
In the present invention, latin of the pallor bacillus isOchrobactrum sp.Latin of the bacillus aryabhattai isBacillus aryabhattaiLatin of the yellow basket bacteria isTalaromyces flavus. The sources of the pallidum, the bacillus arvensis and the panus flavus are not particularly limited, and the conventional commercial strains can be adopted by the person skilled in the art.
In the invention, the fruit tree preferably comprises one or more of apples, pears and walnuts.
In the present invention, the pallidum, the bacillus arvensis and the basket-shaped bacteria are preferably applied to the fruit tree in the form of bacterial liquid. In the invention, the bacterial content of the pallidum in the bacterial liquid is preferably 1-2 multiplied by 10 10 CFU/mL; the bacterial content of the bacillus aryabhattai in the bacterial liquid is preferably 0.5-1.5x10 8 CFU/mL; the preferred bacterial content of the basket-shaped bacteria in the bacterial liquid is 1.2-1.5X10 10 CFU/mL. In the present invention, the content of the pallidum bacteria in the bacterial liquid is more preferably 1.5X10 10 CFU/mL; the bacterial content of the bacillus aryabhattai in the bacterial liquid is more preferably 1 multiplied by 10 8 CFU/mL；
The preferred bacterial content of the basket-like bacteria in the bacterial liquid is 1.3X10 10 CFU/mL. The preparation method of the bacterial liquid is not particularly limited, and bacterial liquid is preferably obtained by adopting a conventional method for culturing the pallidum, the bacillus albopictus and the yellow basket-shaped bacteria, and then the bacterial liquid is obtained by dissolving the bacterial liquid in sterile water and adjusting the average content of the three bacillus.
In the present invention, when the fruit tree is preferably a fruiting tree, the amount of the bacterial liquid applied per fruit tree is preferably 2 to 3L, more preferably 2.5L. In the invention, the bacterial liquid is preferably applied to the root of a fruit tree, and is preferably applied 1 time in spring every year. In the invention, when the fruit tree is preferably a seedling, the amount of the bacterial liquid applied to each fruit tree seedling is preferably 5-10 mL, more preferably 8mL.
The present invention will be described in detail with reference to examples for further illustration of the invention, but they should not be construed as limiting the scope of the invention.
Example 1
Culturing commercial (three strains are all purchased from China center for type culture Collection of microorganisms) pallidum by conventional culture methodOchrobactrum sp.) ACCC 11802 and Bacillus aryabhattaiBacillus aryabhattai) ACCC 06384 and Monilinia flavescensTalaromyces flavus) Obtaining bacteria by ACCC 30404 method, dissolving the obtained bacteria in sterile water, and regulating bacteria content to obtain bacterial liquid, wherein the bacteria content of the pallidum in the bacterial liquid is 1×10 10 CFU/mL, bacillus aryabhattai bacteria content of 1.5X10 8 CFU/mL, the strain content of the wart basket is 1.5X10 10 CFU/mL。
The method adopts conventional planting conditions, takes 8-year-old 'Cuiguan' pears as a research object, applies the prepared bacterial liquid for 1 time in spring, irrigates the bacterial liquid to the root, applies 2.5L of each tree, takes equal amount of sterile water as a control group 1, and contains 2.5 multiplied by 10 of pallidum in the bacterial liquid 10 CFU/mL and Bacillus aryabhattai 2.5X10 8 CFU/mL is used as control group 2, and the bacterial liquid contains equivalent amount of 2.5X10 of pallidum 10 CFU/mL and 2.5X10 of basket 10 CFU/mL as control group 3, eachTreatment of 5 plants, 3 replicates, averaging, and measurement of single fruit weight, soluble solids, and titratable acid content after mature picking of the pears, results are shown in table 1.
TABLE 1 Effect of bacterial liquid treatment on pear fruit
As can be seen from Table 1, compared with the treatment of the control group 1-3, the single fruit weight, the soluble solids and the titratable acid all have significant differences (p < 0.05), and compared with the control group 1, the single fruit weight is improved by 11.3%, the soluble solids are improved by 19.6%, and the titratable acid is reduced by 17.2% by adopting the treatment method of the bacterial liquid, so that the pear quality is remarkably improved.
Example 2
Obtaining bacteria by conventional method for culturing pallidum, bacillus arvensis and Monascus flavus, dissolving the obtained bacteria in sterile water, and regulating bacteria content to obtain bacterial liquid, wherein the bacteria content of pallidum in the bacterial liquid is 2×10 10 CFU/mL, bacillus aryabhattai bacteria content of 0.5X10 8 CFU/mL, the strain content of the wart basket is 1.2X10 10 CFU/mL。
The method comprises the steps of adopting conventional planting conditions, taking Fuji apples of 'Changfu No. 2' as a research object, applying the prepared bacterial liquid for 1 time in spring, irrigating the root, applying 3L of each tree, taking equal amount of sterile water as a control group 1, and taking bacterial liquid containing 2.5X10 of pallidum 10 CFU/mL and Bacillus aryabhattai 2.5X10 8 CFU/mL is used as control group 2, and the bacterial liquid contains equivalent amount of 2.5X10 of pallidum 10 CFU/mL and 2.5X10 of basket 10 CFU/mL was used as control group 3, 10 plants were treated each, 3 replicates were averaged, and the inflorescence fruit setting rate, spring tip length, autumn tip length, individual yield and individual fruit weight were counted, and the results are shown in table 2.
TABLE 2 Effect of bacterial liquid treatment on apples
As can be seen from Table 2, compared with the control group 1-3 treatment, the single plant inflorescence fruit setting rate, spring tip length, autumn tip length, single plant yield and single fruit weight all have significant differences (p is less than 0.05), and compared with the control group 1, the bacterial liquid treatment fruit setting rate of the invention is improved by 16.9%, the spring tip length is increased by 16.8%, the autumn tip length is increased by 15.2%, the single plant yield is improved by 12.7%, and the single fruit weight is improved by 9.6%, so that the bacterial liquid treatment apple of the invention not only increases the yield but also promotes the growth.
Example 3
Obtaining bacteria by conventional method for culturing pallidum, bacillus arvensis and Monascus flavus, dissolving the obtained bacteria in sterile water, and regulating bacteria content to obtain bacterial liquid, wherein the bacteria content of pallidum in the bacterial liquid is 2×10 10 CFU/mL, bacillus aryabhattai bacteria content of 1.0X10 8 CFU/mL, the strain content of the wart basket is 1.2X10 10 CFU/mL。
The test is carried out 3 months later, the conventional management is carried out during the growth period of walnut seedlings, after the walnut seedlings are transplanted to survive, the bacterial liquid 10mL is injected around the rhizosphere of the walnut by using a sterile injector, the test is repeated 10 times, the culture medium (without bacteria) 10mL after each pot injection sterilization is used as a control group 1, and the bacterial liquid contains the pallidum 2.5x10 10 CFU/mL and Bacillus aryabhattai 2.5X10 8 CFU/mL is used as control group 2, and the bacterial liquid contains equivalent amount of 2.5X10 of pallidum 10 CFU/mL and 2.5X10 of basket 10 CFU/mL was used as control group 3. After the walnut seedlings stopped growing in 10 months of the year, the heights of the walnut seedlings were measured, and the results are shown in Table 3.
Table 3 results of walnut seedling height
As can be seen from Table 3, the bacterial liquid provided by the invention significantly improves the growth of walnut seedlings, has significant difference (p < 0.05) with the control group 1-3, and improves the plant height by 20.7% compared with the control group 1.
Although the foregoing embodiments have been described in some, but not all, embodiments of the invention, it should be understood that other embodiments may be devised in accordance with the present embodiments without departing from the spirit and scope of the invention.
Claims (1)
1. A method for promoting growth of fruit trees by rhizosphere soil microorganisms, which is characterized in that pallidum, bacillus arvensis and basket-shaped bacteria are applied to the roots of the fruit trees; the fruit trees are apples or pears; the pallidum, the bacillus albopictus and the panus flavus are applied to fruit trees in the form of bacterial liquid; the content of the pallor bacillus in the bacterial liquid is 1.5X10 10 CFU/mL；
The bacillus aryabhattai in the bacterial liquid has the bacterial content of 1 multiplied by 10 8 CFU/mL；
The fungus content of the yellow basket fungus in the fungus liquid is 1.3X10 10 CFU/mL；
The amount of the fungus liquid applied to each fruit tree is 2-3L;
the bacterial liquid is applied to the root of the fruit tree, and is applied for 1 time in spring every year.
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| CN202310633632.0A CN116458357B (en) | 2023-05-31 | 2023-05-31 | Method for promoting growth of fruit trees by rhizosphere soil microorganisms |
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| CN116458357B true CN116458357B (en) | 2024-02-06 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286383A (en) * | 2011-09-06 | 2011-12-21 | 青岛农业大学 | Talaromyces flavus and application of same in prevention of plant pathogens |
| CN104286032A (en) * | 2014-09-04 | 2015-01-21 | 青岛农业大学 | Preparation method of Talaromyces flavus spore powder, Talaromyces flavus wettable pulvis and preparation method of wettable pulvis |
| CN105420136A (en) * | 2014-09-17 | 2016-03-23 | 中国农业科学院植物保护研究所 | Ochrobactrum oryzae strain for prevention and control of plant root cancer and application thereof |
| CN105670955A (en) * | 2015-10-14 | 2016-06-15 | 四川农业大学 | Ochrobacterum sp. MGJ11, and method of immobilizing heavy metal cadmium in soil therewith |
| CN112322540A (en) * | 2019-11-14 | 2021-02-05 | 中国农业科学院农业资源与农业区划研究所 | Ochrobactrum and application thereof, and microbial inoculum and application thereof in degrading antibiotics |
| CN112410244A (en) * | 2020-09-30 | 2021-02-26 | 山东农业大学 | Bacillus aryabhattai and application thereof |
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- 2023-05-31 CN CN202310633632.0A patent/CN116458357B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286383A (en) * | 2011-09-06 | 2011-12-21 | 青岛农业大学 | Talaromyces flavus and application of same in prevention of plant pathogens |
| CN104286032A (en) * | 2014-09-04 | 2015-01-21 | 青岛农业大学 | Preparation method of Talaromyces flavus spore powder, Talaromyces flavus wettable pulvis and preparation method of wettable pulvis |
| CN105420136A (en) * | 2014-09-17 | 2016-03-23 | 中国农业科学院植物保护研究所 | Ochrobactrum oryzae strain for prevention and control of plant root cancer and application thereof |
| CN105670955A (en) * | 2015-10-14 | 2016-06-15 | 四川农业大学 | Ochrobacterum sp. MGJ11, and method of immobilizing heavy metal cadmium in soil therewith |
| CN112322540A (en) * | 2019-11-14 | 2021-02-05 | 中国农业科学院农业资源与农业区划研究所 | Ochrobactrum and application thereof, and microbial inoculum and application thereof in degrading antibiotics |
| CN112410244A (en) * | 2020-09-30 | 2021-02-26 | 山东农业大学 | Bacillus aryabhattai and application thereof |
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